Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70958.t1 | XP_003614456.2 | 93.2 | 752 | 36 | 4 | 1 | 737 | 1 | 752 | 0.00E+00 | 1387.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70958.t1 | B6SFA4 | 57.6 | 766 | 287 | 11 | 1 | 737 | 1 | 757 | 2.0e-244 | 846.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70958.t1 | G7JY95 | 93.2 | 752 | 36 | 4 | 1 | 737 | 1 | 752 | 0.0e+00 | 1387.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052414 | MS.gene70958 | 0.801052 | 1.08E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70958.t1 | MTR_5g053930 | 93.218 | 752 | 36 | 4 | 1 | 737 | 1 | 752 | 0.0 | 1440 |
| MS.gene70958.t1 | MTR_1g052700 | 96.198 | 263 | 10 | 0 | 475 | 737 | 44 | 306 | 0.0 | 535 |
| MS.gene70958.t1 | MTR_8g020390 | 43.934 | 305 | 169 | 2 | 434 | 737 | 459 | 762 | 8.45e-74 | 257 |
| MS.gene70958.t1 | MTR_8g020390 | 43.934 | 305 | 169 | 2 | 434 | 737 | 747 | 1050 | 1.62e-72 | 256 |
| MS.gene70958.t1 | MTR_8g020390 | 43.934 | 305 | 169 | 2 | 434 | 737 | 964 | 1267 | 5.32e-72 | 256 |
| MS.gene70958.t1 | MTR_2g105760 | 39.024 | 369 | 191 | 6 | 403 | 738 | 1707 | 2074 | 3.18e-71 | 254 |
| MS.gene70958.t1 | MTR_2g105760 | 27.500 | 400 | 255 | 12 | 17 | 393 | 1231 | 1618 | 1.14e-30 | 130 |
| MS.gene70958.t1 | MTR_5g076300 | 37.954 | 303 | 181 | 7 | 435 | 733 | 552 | 851 | 5.14e-49 | 186 |
| MS.gene70958.t1 | MTR_5g076330 | 37.086 | 302 | 185 | 5 | 435 | 733 | 527 | 826 | 5.72e-49 | 186 |
| MS.gene70958.t1 | MTR_3g064210 | 38.188 | 309 | 176 | 8 | 434 | 733 | 608 | 910 | 1.17e-46 | 179 |
| MS.gene70958.t1 | MTR_5g076440 | 35.526 | 304 | 188 | 7 | 435 | 733 | 541 | 841 | 1.25e-46 | 179 |
| MS.gene70958.t1 | MTR_5g076310 | 35.235 | 298 | 188 | 5 | 440 | 733 | 512 | 808 | 2.70e-46 | 177 |
| MS.gene70958.t1 | MTR_5g013820 | 29.577 | 426 | 255 | 12 | 334 | 733 | 500 | 906 | 5.73e-45 | 174 |
| MS.gene70958.t1 | MTR_5g013820 | 29.577 | 426 | 255 | 12 | 334 | 733 | 500 | 906 | 6.04e-45 | 174 |
| MS.gene70958.t1 | MTR_0189s0030 | 34.076 | 314 | 195 | 6 | 431 | 737 | 517 | 825 | 1.99e-44 | 173 |
| MS.gene70958.t1 | MTR_3g064190 | 34.323 | 303 | 193 | 6 | 435 | 733 | 531 | 831 | 2.92e-44 | 172 |
| MS.gene70958.t1 | MTR_5g076380 | 35.117 | 299 | 186 | 7 | 440 | 733 | 535 | 830 | 4.28e-44 | 171 |
| MS.gene70958.t1 | MTR_3g436400 | 31.511 | 311 | 209 | 4 | 430 | 737 | 537 | 846 | 6.48e-41 | 162 |
| MS.gene70958.t1 | MTR_5g076320 | 34.868 | 304 | 177 | 7 | 440 | 736 | 559 | 848 | 1.52e-40 | 160 |
| MS.gene70958.t1 | MTR_5g076500 | 32.886 | 298 | 177 | 7 | 440 | 733 | 532 | 810 | 1.13e-37 | 151 |
| MS.gene70958.t1 | MTR_1g040155 | 29.295 | 454 | 254 | 12 | 316 | 724 | 450 | 881 | 2.91e-36 | 147 |
| MS.gene70958.t1 | MTR_7g092200 | 31.026 | 419 | 212 | 14 | 354 | 725 | 240 | 628 | 1.68e-31 | 131 |
| MS.gene70958.t1 | MTR_2g105760 | 27.228 | 404 | 259 | 12 | 13 | 393 | 1121 | 1512 | 9.61e-31 | 130 |
| MS.gene70958.t1 | MTR_2g105760 | 37.179 | 156 | 76 | 3 | 403 | 536 | 1601 | 1756 | 1.62e-20 | 97.8 |
| MS.gene70958.t1 | MTR_2g105760 | 27.500 | 400 | 255 | 12 | 17 | 393 | 1231 | 1618 | 1.38e-30 | 130 |
| MS.gene70958.t1 | MTR_2g105760 | 37.179 | 156 | 76 | 3 | 403 | 536 | 1707 | 1862 | 1.61e-20 | 97.8 |
| MS.gene70958.t1 | MTR_2g049990 | 29.703 | 404 | 212 | 17 | 353 | 724 | 185 | 548 | 2.44e-29 | 124 |
| MS.gene70958.t1 | MTR_5g006890 | 29.280 | 403 | 215 | 16 | 353 | 724 | 185 | 548 | 5.05e-29 | 124 |
| MS.gene70958.t1 | MTR_2g049990 | 29.703 | 404 | 212 | 17 | 353 | 724 | 444 | 807 | 5.93e-29 | 124 |
| MS.gene70958.t1 | MTR_5g006890 | 29.529 | 403 | 214 | 17 | 353 | 724 | 444 | 807 | 9.93e-29 | 123 |
| MS.gene70958.t1 | MTR_1g038815 | 23.263 | 331 | 217 | 12 | 430 | 738 | 1090 | 1405 | 7.77e-13 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70958.t1 | AT4G15570 | 57.702 | 766 | 286 | 11 | 1 | 737 | 1 | 757 | 0.0 | 879 |
| MS.gene70958.t1 | AT4G15570 | 57.702 | 766 | 286 | 11 | 1 | 737 | 1 | 757 | 0.0 | 879 |
| MS.gene70958.t1 | AT4G15570 | 59.731 | 668 | 241 | 7 | 91 | 737 | 8 | 668 | 0.0 | 814 |
| MS.gene70958.t1 | AT4G15570 | 59.731 | 668 | 241 | 7 | 91 | 737 | 8 | 668 | 0.0 | 814 |
| MS.gene70958.t1 | AT2G19120 | 44.300 | 307 | 169 | 2 | 434 | 739 | 744 | 1049 | 9.18e-74 | 259 |
| MS.gene70958.t1 | AT4G30100 | 44.300 | 307 | 169 | 2 | 434 | 739 | 907 | 1212 | 1.48e-71 | 255 |
| MS.gene70958.t1 | AT4G30100 | 44.300 | 307 | 169 | 2 | 434 | 739 | 907 | 1212 | 1.48e-71 | 255 |
| MS.gene70958.t1 | AT1G16800 | 41.104 | 326 | 167 | 4 | 435 | 736 | 1560 | 1884 | 1.66e-70 | 252 |
| MS.gene70958.t1 | AT1G16800 | 27.612 | 402 | 247 | 14 | 17 | 393 | 1044 | 1426 | 6.62e-32 | 134 |
| MS.gene70958.t1 | AT1G16800 | 38.187 | 364 | 195 | 5 | 402 | 736 | 1564 | 1926 | 1.74e-70 | 252 |
| MS.gene70958.t1 | AT1G16800 | 27.612 | 402 | 247 | 14 | 17 | 393 | 1086 | 1468 | 6.96e-32 | 134 |
| MS.gene70958.t1 | AT1G16800 | 38.187 | 364 | 195 | 5 | 402 | 736 | 1634 | 1996 | 1.76e-70 | 252 |
| MS.gene70958.t1 | AT1G16800 | 27.612 | 402 | 247 | 14 | 17 | 393 | 1156 | 1538 | 6.39e-32 | 134 |
| MS.gene70958.t1 | AT1G65810 | 36.093 | 302 | 186 | 5 | 435 | 733 | 526 | 823 | 1.76e-48 | 185 |
| MS.gene70958.t1 | AT1G65810 | 36.093 | 302 | 186 | 5 | 435 | 733 | 526 | 823 | 2.04e-48 | 185 |
| MS.gene70958.t1 | AT1G65810 | 36.093 | 302 | 186 | 5 | 435 | 733 | 526 | 823 | 2.17e-48 | 185 |
| MS.gene70958.t1 | AT5G37030 | 31.234 | 397 | 234 | 11 | 352 | 734 | 250 | 621 | 3.27e-46 | 175 |
| MS.gene70958.t1 | AT5G47010 | 29.673 | 428 | 256 | 12 | 334 | 735 | 512 | 920 | 6.40e-45 | 174 |
| MS.gene70958.t1 | AT5G37165 | 33.974 | 312 | 196 | 8 | 430 | 734 | 79 | 387 | 1.38e-43 | 163 |
| MS.gene70958.t1 | AT4G05540 | 34.859 | 284 | 179 | 5 | 435 | 714 | 402 | 683 | 4.86e-41 | 160 |
| MS.gene70958.t1 | AT5G37150 | 31.818 | 308 | 203 | 7 | 432 | 734 | 495 | 800 | 1.86e-40 | 160 |
| MS.gene70958.t1 | AT5G52090 | 31.270 | 307 | 204 | 7 | 432 | 733 | 332 | 636 | 2.07e-39 | 155 |
| MS.gene70958.t1 | AT5G35970 | 30.176 | 454 | 250 | 14 | 316 | 724 | 498 | 929 | 5.79e-39 | 155 |
| MS.gene70958.t1 | AT5G37140 | 32.468 | 308 | 197 | 9 | 431 | 734 | 353 | 653 | 3.76e-38 | 152 |
| MS.gene70958.t1 | AT1G65780 | 34.921 | 315 | 190 | 8 | 428 | 733 | 530 | 838 | 3.85e-36 | 147 |
| MS.gene70958.t1 | AT2G03270 | 31.059 | 425 | 226 | 16 | 343 | 725 | 224 | 623 | 1.84e-30 | 128 |
| MS.gene70958.t1 | AT1G05460 | 27.791 | 421 | 240 | 15 | 334 | 724 | 423 | 809 | 2.04e-29 | 125 |
| MS.gene70958.t1 | AT1G05460 | 27.791 | 421 | 240 | 15 | 334 | 724 | 423 | 809 | 4.57e-29 | 124 |
Find 164 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTATCCGAGCTGCAATTT+TGG | 0.127352 | 3.3:+44534876 | MS.gene70958:CDS |
| TGTATATCAAAGAAGGAAAA+TGG | 0.214241 | 3.3:-44538687 | None:intergenic |
| AATGCTGTTGGAGAGGATTT+TGG | 0.237829 | 3.3:+44527147 | MS.gene70958:CDS |
| AGTCTAACTCGAAGAACTTT+TGG | 0.280001 | 3.3:-44523865 | None:intergenic |
| CGAGTTGAAGTTACAGCTTC+AGG | 0.281533 | 3.3:-44531128 | None:intergenic |
| GTTATGGCCTCACGTTGATA+TGG | 0.307471 | 3.3:-44527234 | None:intergenic |
| CTTAGCAATGTCCGAAATTA+CGG | 0.317425 | 3.3:-44537223 | None:intergenic |
| ACTAATTCATTTCCAGTAGT+TGG | 0.317580 | 3.3:-44531029 | None:intergenic |
| CCTTGATGAGATTGTGTTGC+GGG | 0.327991 | 3.3:+44531196 | MS.gene70958:CDS |
| ATTCTTGACGTTGACGAGTT+TGG | 0.329524 | 3.3:-44521853 | None:intergenic |
| GCAAGTAAAGAAGGAGGTAT+AGG | 0.337274 | 3.3:+44540231 | MS.gene70958:CDS |
| AAAACTTACTTCTCCTCAAT+TGG | 0.338467 | 3.3:-44529112 | None:intergenic |
| TTCTCCACCCTCAGCTTTAG+TGG | 0.341391 | 3.3:+44535001 | MS.gene70958:CDS |
| CATATTCATCCAAAGGTAAT+GGG | 0.345623 | 3.3:-44527194 | None:intergenic |
| GCCACGAATGATTTGTGATT+TGG | 0.347715 | 3.3:-44521934 | None:intergenic |
| ACTTCCAGCAACCGTAATTT+CGG | 0.348308 | 3.3:+44537212 | MS.gene70958:CDS |
| CTACAAGATTATTCTTGCTT+GGG | 0.362323 | 3.3:+44521675 | MS.gene70958:CDS |
| TTTAATATTAAATCCTTGAA+TGG | 0.362438 | 3.3:-44527126 | None:intergenic |
| CTAATTCATTTCCAGTAGTT+GGG | 0.387171 | 3.3:-44531028 | None:intergenic |
| GCCAAGGGAACAAGAGTTGC+TGG | 0.388466 | 3.3:-44536748 | None:intergenic |
| CAAGTAAAGAAGGAGGTATA+GGG | 0.388484 | 3.3:+44540232 | MS.gene70958:CDS |
| GCAGGGGCTGAATGGAAGTT+AGG | 0.388571 | 3.3:+44523550 | MS.gene70958:intron |
| ATGGAAAATCCCATTACCTT+TGG | 0.389403 | 3.3:+44527185 | MS.gene70958:CDS |
| TACTTTGACTGTACTCTCAT+TGG | 0.390762 | 3.3:-44528814 | None:intergenic |
| TCTACAAGATTATTCTTGCT+TGG | 0.394427 | 3.3:+44521674 | MS.gene70958:CDS |
| TCGGACATTGCTAAGAATCA+TGG | 0.397017 | 3.3:+44537231 | MS.gene70958:CDS |
| TACGCATGCATTTGCTTTGG+TGG | 0.401294 | 3.3:+44523819 | MS.gene70958:CDS |
| TGGCATAGGTACCGCTGCTT+TGG | 0.403022 | 3.3:+44538663 | MS.gene70958:CDS |
| GGAAAAGAAGCTAAGCCATC+TGG | 0.403749 | 3.3:+44538714 | MS.gene70958:CDS |
| CGTTGTAATGGCATTGGATA+AGG | 0.411334 | 3.3:+44521618 | None:intergenic |
| ACATATTCATCCAAAGGTAA+TGG | 0.411540 | 3.3:-44527195 | None:intergenic |
| TACTACGCATGCATTTGCTT+TGG | 0.415462 | 3.3:+44523816 | MS.gene70958:CDS |
| ACCAGCAACTCTTGTTCCCT+TGG | 0.421417 | 3.3:+44536747 | MS.gene70958:CDS |
| AGAAGGAGGTATAGGGTTTG+TGG | 0.421995 | 3.3:+44540239 | MS.gene70958:CDS |
| GGGAAGACTCAGACCATACT+TGG | 0.427450 | 3.3:+44528763 | MS.gene70958:CDS |
| ATGTCCGAAATTACGGTTGC+TGG | 0.427872 | 3.3:-44537216 | None:intergenic |
| TTGTGTTTCTGTAGGTGGTT+GGG | 0.428362 | 3.3:+44540385 | MS.gene70958:intron |
| TATGACAATTCCTTGGAAGA+TGG | 0.433987 | 3.3:+44538609 | MS.gene70958:CDS |
| CTTATTTCAAAGACTGATGA+AGG | 0.435815 | 3.3:+44537368 | MS.gene70958:CDS |
| TGCCCGAAAATTGTGAGGAT+TGG | 0.442101 | 3.3:+44532107 | MS.gene70958:CDS |
| AGTACTCAGAATCCCAAGTA+TGG | 0.447921 | 3.3:-44528776 | None:intergenic |
| TCTAGAAAATGAAAGTCATC+TGG | 0.447956 | 3.3:-44523601 | None:intergenic |
| ATGACAATTCCTTGGAAGAT+GGG | 0.453665 | 3.3:+44538610 | MS.gene70958:CDS |
| TTAATATTAAATCCTTGAAT+GGG | 0.453919 | 3.3:-44527125 | None:intergenic |
| GATTTAATATTAAATGCTGT+TGG | 0.457174 | 3.3:+44527135 | MS.gene70958:CDS |
| GGGAAACAGCCTTGATAGAA+TGG | 0.465367 | 3.3:-44532142 | None:intergenic |
| GTTGTGTTTCTGTAGGTGGT+TGG | 0.468114 | 3.3:+44540384 | MS.gene70958:intron |
| AGCCATCTGGGAGTGGTTCG+TGG | 0.468957 | 3.3:+44538727 | MS.gene70958:CDS |
| AAATACAGACAGAACGTGAT+TGG | 0.471495 | 3.3:+44538643 | MS.gene70958:CDS |
| CTTCAAAACGTTGTTTGAAG+AGG | 0.472157 | 3.3:-44538869 | None:intergenic |
| TTTAGGGGCCTCCGGGTACT+GGG | 0.472421 | 3.3:+44528743 | MS.gene70958:intron |
| TTGTGGAAAGTGCTGAGAAA+AGG | 0.473365 | 3.3:+44540448 | MS.gene70958:CDS |
| TGGTGATTGCAGGGGCTGAA+TGG | 0.481202 | 3.3:+44523542 | MS.gene70958:intron |
| GTGGATATATGTACCGTTGA+TGG | 0.485702 | 3.3:+44538921 | MS.gene70958:CDS |
| TTTGACATCAGACTATCCCT+TGG | 0.488933 | 3.3:-44530984 | None:intergenic |
| ACGGTTGCTGGAAGTTGAGC+TGG | 0.489120 | 3.3:-44537204 | None:intergenic |
| TCTCCCTCTTCCATATCAAA+AGG | 0.490422 | 3.3:-44523628 | None:intergenic |
| TTTCAAAGACTGATGAAGGC+TGG | 0.492580 | 3.3:+44537372 | MS.gene70958:CDS |
| TCCCTCGTGTATATCAAAGA+AGG | 0.493198 | 3.3:-44538694 | None:intergenic |
| TGTGGAAAGTGCTGAGAAAA+GGG | 0.495177 | 3.3:+44540449 | MS.gene70958:CDS |
| CAGGGGCTGAATGGAAGTTA+GGG | 0.498189 | 3.3:+44523551 | MS.gene70958:intron |
| GGTGCCACATTTACCAATTG+AGG | 0.502235 | 3.3:+44529099 | MS.gene70958:CDS |
| ACATTCGACGAATGAACGTT+GGG | 0.502885 | 3.3:+44540265 | MS.gene70958:CDS |
| CTTGATTTCACATTATCTCC+AGG | 0.503491 | 3.3:-44523895 | None:intergenic |
| GTATATCAAAGAAGGAAAAT+GGG | 0.504159 | 3.3:-44538686 | None:intergenic |
| TTGGTTTCCTGACTTGAGTG+TGG | 0.505908 | 3.3:-44538811 | None:intergenic |
| CGTTATCCCACACTCAAGTC+AGG | 0.506855 | 3.3:+44538804 | MS.gene70958:CDS |
| TTCTCCTCAATTGGTAAATG+TGG | 0.506985 | 3.3:-44529103 | None:intergenic |
| GTCTGAGTCTTCCCAGTACC+CGG | 0.509954 | 3.3:-44528754 | None:intergenic |
| TTCAAGAATGAGAGGCTCAT+AGG | 0.510740 | 3.3:-44521907 | None:intergenic |
| GATAAGAAGCTTTCCCTCGA+TGG | 0.510778 | 3.3:+44538581 | MS.gene70958:intron |
| ATGTACCGTTGATGGTTGCC+AGG | 0.512311 | 3.3:+44538929 | MS.gene70958:CDS |
| CTGGAGATAATGTGAAATCA+AGG | 0.514308 | 3.3:+44523896 | MS.gene70958:CDS |
| TTGACGAGTTTGGAAGTAGT+AGG | 0.517300 | 3.3:-44521843 | None:intergenic |
| TCATCAAGGGCAGAATTTGA+AGG | 0.517544 | 3.3:-44531182 | None:intergenic |
| ATACTCCGTTGTAATGGCAT+TGG | 0.519460 | 3.3:+44521612 | None:intergenic |
| GATAAGCAATCTCCCATTCA+AGG | 0.520613 | 3.3:+44527113 | MS.gene70958:CDS |
| CGTGTCAGCAGAGAAATTAG+TGG | 0.521131 | 3.3:+44538902 | MS.gene70958:CDS |
| CAAAGCACACCATTCTATCA+AGG | 0.522470 | 3.3:+44532133 | MS.gene70958:CDS |
| AGAATAAGAGAAGAAACAAT+GGG | 0.524091 | 3.3:+44521817 | MS.gene70958:intron |
| TACCATTGCTCGTGAGTATG+TGG | 0.525419 | 3.3:+44527080 | MS.gene70958:CDS |
| TGTGTCAGAGCAAGTAAAGA+AGG | 0.527547 | 3.3:+44540222 | MS.gene70958:CDS |
| CGAGTTAGACTCTATCTACC+TGG | 0.529559 | 3.3:+44523877 | MS.gene70958:CDS |
| CGTTCTCATATAAGCGTGGA+AGG | 0.530537 | 3.3:+44523931 | MS.gene70958:CDS |
| AAGCAGAATAATGCTTCTGC+AGG | 0.530730 | 3.3:+44534841 | MS.gene70958:CDS |
| GCCAAATCACAAATCATTCG+TGG | 0.533111 | 3.3:+44521933 | MS.gene70958:CDS |
| TGACAATTCCTTGGAAGATG+GGG | 0.533541 | 3.3:+44538611 | MS.gene70958:CDS |
| GTTACCTTCATAAAATGCAA+TGG | 0.534157 | 3.3:-44523958 | None:intergenic |
| CAGAGCAAAGTCTGCAGTGT+TGG | 0.535018 | 3.3:+44540293 | MS.gene70958:CDS |
| GACGCCCTCCCCATCTTCCA+AGG | 0.536450 | 3.3:-44538619 | None:intergenic |
| AATATTAAATGCTGTTGGAG+AGG | 0.537878 | 3.3:+44527140 | MS.gene70958:CDS |
| TCAACATACAAGTTCGTCTA+GGG | 0.540007 | 3.3:-44532162 | None:intergenic |
| AATTATGATTTCAAATGAAG+AGG | 0.542728 | 3.3:+44523672 | MS.gene70958:CDS |
| CGGACATTGCTAAGAATCAT+GGG | 0.547878 | 3.3:+44537232 | MS.gene70958:CDS |
| TTGGCTTCTTCAAGAATGAG+AGG | 0.548551 | 3.3:-44521915 | None:intergenic |
| CTTACCAGTAGATGATATGT+TGG | 0.549866 | 3.3:-44534821 | None:intergenic |
| CATCAAGGGCAGAATTTGAA+GGG | 0.551014 | 3.3:-44531181 | None:intergenic |
| CAACACTGCAGACTTTGCTC+TGG | 0.551458 | 3.3:-44540292 | None:intergenic |
| TTAGCATCTCCATGGTTACA+TGG | 0.551718 | 3.3:+44530954 | MS.gene70958:CDS |
| GAAAAGAAGCTAAGCCATCT+GGG | 0.555585 | 3.3:+44538715 | MS.gene70958:CDS |
| TTGGTTCAGAAGCTGAAGGA+TGG | 0.555790 | 3.3:+44527166 | MS.gene70958:CDS |
| GGTTACATGGCATCAATCCA+AGG | 0.557352 | 3.3:+44530967 | MS.gene70958:CDS |
| CGGGACCTTCGAGCTAAAGC+AGG | 0.558038 | 3.3:+44529078 | MS.gene70958:CDS |
| AGTGCCAACATATCATCTAC+TGG | 0.559414 | 3.3:+44534817 | MS.gene70958:CDS |
| GACATTCGACGAATGAACGT+TGG | 0.561066 | 3.3:+44540264 | MS.gene70958:CDS |
| GATAGTCTGATGTCAAAAGA+TGG | 0.563014 | 3.3:+44530990 | MS.gene70958:CDS |
| GAGTGGTTCGTGGGTAAATG+TGG | 0.564966 | 3.3:+44538737 | MS.gene70958:CDS |
| CCCGCAACACAATCTCATCA+AGG | 0.565417 | 3.3:-44531196 | None:intergenic |
| CCCTTGATGAGATTGTGTTG+CGG | 0.565992 | 3.3:+44531195 | MS.gene70958:CDS |
| AGGTTGACTTGTCCACTGTA+GGG | 0.569940 | 3.3:-44538849 | None:intergenic |
| CAGAATAAGAGAAGAAACAA+TGG | 0.570369 | 3.3:+44521816 | MS.gene70958:intron |
| CCTTGAAATACATACAATTG+TGG | 0.570703 | 3.3:-44534905 | None:intergenic |
| ATTCGTGGCAACGAAGATGA+AGG | 0.577431 | 3.3:+44521948 | MS.gene70958:CDS |
| GAAGCTAAGCCATCTGGGAG+TGG | 0.580298 | 3.3:+44538720 | MS.gene70958:CDS |
| TGGTTTCCTGACTTGAGTGT+GGG | 0.582456 | 3.3:-44538810 | None:intergenic |
| AGACAGAACGTGATTGGCAT+AGG | 0.583872 | 3.3:+44538649 | MS.gene70958:CDS |
| CAATTCCTTGGAAGATGGGG+AGG | 0.584683 | 3.3:+44538614 | MS.gene70958:CDS |
| TGGCACCTGCTTTAGCTCGA+AGG | 0.585824 | 3.3:-44529083 | None:intergenic |
| ATGTCAAAAGATGGTGATGA+TGG | 0.587988 | 3.3:+44530999 | MS.gene70958:CDS |
| ATCAACATACAAGTTCGTCT+AGG | 0.588573 | 3.3:-44532163 | None:intergenic |
| TTTCACTCTTACAGCTTGTG+CGG | 0.591093 | 3.3:-44535080 | None:intergenic |
| GCCATCTGGGAGTGGTTCGT+GGG | 0.591835 | 3.3:+44538728 | MS.gene70958:CDS |
| TTGGGTCTGCCTCAACATTG+AGG | 0.592122 | 3.3:+44540403 | MS.gene70958:CDS |
| ATTGGAGATTAGCATCTCCA+TGG | 0.592971 | 3.3:+44530946 | MS.gene70958:CDS |
| CATTGAGGAGGAACGTACAA+TGG | 0.594093 | 3.3:+44540418 | MS.gene70958:CDS |
| CTTGTAGAAACGATGAATTG+TGG | 0.595441 | 3.3:-44521659 | None:intergenic |
| TGGTTCGTGGGTAAATGTGG+AGG | 0.598793 | 3.3:+44538740 | MS.gene70958:CDS |
| AGGAAAATGGGCCAAAGCAG+CGG | 0.598942 | 3.3:-44538674 | None:intergenic |
| AGGGTGATATAATTGCAACT+TGG | 0.601841 | 3.3:-44538830 | None:intergenic |
| CTTATTGCCCGAAAATTGTG+AGG | 0.610509 | 3.3:+44532102 | MS.gene70958:CDS |
| AGCCACATACTCACGAGCAA+TGG | 0.612987 | 3.3:-44527082 | None:intergenic |
| TTGTCATAAAACTCCATCGA+GGG | 0.615125 | 3.3:-44538594 | None:intergenic |
| GTGTTGCGGGTTCTCAGTGG+AGG | 0.616673 | 3.3:+44531209 | MS.gene70958:CDS |
| GTTTACCTGGCAACCATCAA+CGG | 0.618399 | 3.3:-44538934 | None:intergenic |
| TACGTTCCTCCTCAATGTTG+AGG | 0.620686 | 3.3:-44540412 | None:intergenic |
| TCATTAACATATTCATCCAA+AGG | 0.625155 | 3.3:-44527201 | None:intergenic |
| TCCTTCTTTGATATACACGA+GGG | 0.626412 | 3.3:+44538693 | MS.gene70958:CDS |
| GAGGTTGACTTGTCCACTGT+AGG | 0.627968 | 3.3:-44538850 | None:intergenic |
| CGTACAATGGAACAAGCTTG+TGG | 0.630942 | 3.3:+44540431 | MS.gene70958:CDS |
| CCTCATCCAAAATTGCAGCT+CGG | 0.631412 | 3.3:-44534882 | None:intergenic |
| TTCCTTCTTTGATATACACG+AGG | 0.631750 | 3.3:+44538692 | MS.gene70958:CDS |
| ACCCACGAACCACTCCCAGA+TGG | 0.632201 | 3.3:-44538729 | None:intergenic |
| CATTTGAACCACTAAAGCTG+AGG | 0.635489 | 3.3:-44535009 | None:intergenic |
| GGAAGACTCAGACCATACTT+GGG | 0.638948 | 3.3:+44528764 | MS.gene70958:CDS |
| CCGCAACACAATCTCATCAA+GGG | 0.642678 | 3.3:-44531195 | None:intergenic |
| AGGGGCTGAATGGAAGTTAG+GGG | 0.646588 | 3.3:+44523552 | MS.gene70958:intron |
| GTTACATGGCATCAATCCAA+GGG | 0.646769 | 3.3:+44530968 | MS.gene70958:CDS |
| GTCAGAGCAAGTAAAGAAGG+AGG | 0.652708 | 3.3:+44540225 | MS.gene70958:CDS |
| ACAAACCTGTATCAGAACAA+AGG | 0.657049 | 3.3:-44528059 | None:intergenic |
| ATTTGAACCACTAAAGCTGA+GGG | 0.657833 | 3.3:-44535008 | None:intergenic |
| AATGCGTTCTCATATAAGCG+TGG | 0.659078 | 3.3:+44523927 | MS.gene70958:CDS |
| AATTCCTTGGAAGATGGGGA+GGG | 0.659906 | 3.3:+44538615 | MS.gene70958:CDS |
| ATCAAGGGCAGAATTTGAAG+GGG | 0.661980 | 3.3:-44531180 | None:intergenic |
| TTTCAATCCATATCAACGTG+AGG | 0.678135 | 3.3:+44527227 | MS.gene70958:CDS |
| ATTGTGTTGCGGGTTCTCAG+TGG | 0.681210 | 3.3:+44531206 | MS.gene70958:CDS |
| TGAACCACTAAAGCTGAGGG+TGG | 0.681993 | 3.3:-44535005 | None:intergenic |
| GGCTGAATGGAAGTTAGGGG+CGG | 0.682780 | 3.3:+44523555 | MS.gene70958:CDS |
| GGATTGATGCCATGTAACCA+TGG | 0.688224 | 3.3:-44530963 | None:intergenic |
| GGTCTGCCTCAACATTGAGG+AGG | 0.690370 | 3.3:+44540406 | MS.gene70958:CDS |
| TGAGTCTTCCCAGTACCCGG+AGG | 0.696404 | 3.3:-44528751 | None:intergenic |
| GTTCTCATATAAGCGTGGAA+GGG | 0.703735 | 3.3:+44523932 | MS.gene70958:CDS |
| GCAATTATATCACCCTACAG+TGG | 0.715002 | 3.3:+44538837 | MS.gene70958:CDS |
| ACAATATCGAATGCATCCTG+AGG | 0.724850 | 3.3:+44537419 | MS.gene70958:CDS |
| ATTGTCATAAAACTCCATCG+AGG | 0.776280 | 3.3:-44538595 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 44521625 | 44540521 | 44521625 | ID=MS.gene70958 |
| chr3.3 | mRNA | 44521625 | 44540521 | 44521625 | ID=MS.gene70958.t1;Parent=MS.gene70958 |
| chr3.3 | exon | 44521625 | 44521702 | 44521625 | ID=MS.gene70958.t1.exon1;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44521625 | 44521702 | 44521625 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44521819 | 44521969 | 44521819 | ID=MS.gene70958.t1.exon2;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44521819 | 44521969 | 44521819 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44523554 | 44523693 | 44523554 | ID=MS.gene70958.t1.exon3;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44523554 | 44523693 | 44523554 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44523802 | 44523975 | 44523802 | ID=MS.gene70958.t1.exon4;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44523802 | 44523975 | 44523802 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44527066 | 44527257 | 44527066 | ID=MS.gene70958.t1.exon5;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44527066 | 44527257 | 44527066 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44528040 | 44528075 | 44528040 | ID=MS.gene70958.t1.exon6;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44528040 | 44528075 | 44528040 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44528748 | 44528833 | 44528748 | ID=MS.gene70958.t1.exon7;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44528748 | 44528833 | 44528748 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44529075 | 44529125 | 44529075 | ID=MS.gene70958.t1.exon8;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44529075 | 44529125 | 44529075 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44530938 | 44531049 | 44530938 | ID=MS.gene70958.t1.exon9;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44530938 | 44531049 | 44530938 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44531125 | 44531230 | 44531125 | ID=MS.gene70958.t1.exon10;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44531125 | 44531230 | 44531125 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44532078 | 44532175 | 44532078 | ID=MS.gene70958.t1.exon11;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44532078 | 44532175 | 44532078 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44534802 | 44534912 | 44534802 | ID=MS.gene70958.t1.exon12;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44534802 | 44534912 | 44534802 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44534998 | 44535090 | 44534998 | ID=MS.gene70958.t1.exon13;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44534998 | 44535090 | 44534998 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44536742 | 44536795 | 44536742 | ID=MS.gene70958.t1.exon14;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44536742 | 44536795 | 44536742 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44537195 | 44537253 | 44537195 | ID=MS.gene70958.t1.exon15;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44537195 | 44537253 | 44537195 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44537356 | 44537440 | 44537356 | ID=MS.gene70958.t1.exon16;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44537356 | 44537440 | 44537356 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44538582 | 44538950 | 44538582 | ID=MS.gene70958.t1.exon17;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44538582 | 44538950 | 44538582 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44540192 | 44540314 | 44540192 | ID=MS.gene70958.t1.exon18;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44540192 | 44540314 | 44540192 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| chr3.3 | exon | 44540399 | 44540521 | 44540399 | ID=MS.gene70958.t1.exon19;Parent=MS.gene70958.t1 |
| chr3.3 | CDS | 44540399 | 44540521 | 44540399 | ID=cds.MS.gene70958.t1;Parent=MS.gene70958.t1 |
| Gene Sequence |
| Protein sequence |
