Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71605.t1 | XP_013469974.1 | 83.7 | 196 | 27 | 4 | 1 | 191 | 3 | 198 | 4.60E-83 | 317.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71605.t1 | A0A072VQE3 | 83.7 | 196 | 27 | 4 | 1 | 191 | 3 | 198 | 3.3e-83 | 317.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053581 | MS.gene71605 | 0.817789 | 2.75E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71605.t1 | MTR_1g106080 | 92.347 | 196 | 10 | 4 | 1 | 191 | 3 | 198 | 1.27e-117 | 332 |
| MS.gene71605.t1 | MTR_5g005230 | 50.510 | 196 | 72 | 11 | 1 | 187 | 1 | 180 | 2.98e-41 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71605.t1 | AT4G33800 | 39.655 | 174 | 82 | 8 | 7 | 169 | 3 | 164 | 6.32e-22 | 87.8 |
| MS.gene71605.t1 | AT4G33800 | 39.655 | 174 | 82 | 8 | 7 | 169 | 3 | 164 | 6.32e-22 | 87.8 |
Find 29 sgRNAs with CRISPR-Local
Find 42 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGGTTTCTGATGCTTCTTC+TGG | 0.266605 | 1.4:-82086274 | MS.gene71605:CDS |
| AACATGAGTGCATTTGATTC+TGG | 0.312778 | 1.4:-82086451 | MS.gene71605:CDS |
| TCATACCAGGAAGAACAATT+TGG | 0.315479 | 1.4:-82085759 | MS.gene71605:CDS |
| ACAAAGTTATGTAGGAAATA+TGG | 0.330154 | 1.4:-82085921 | MS.gene71605:CDS |
| TATGAAAACACTAGATTCAT+AGG | 0.343147 | 1.4:-82086379 | MS.gene71605:CDS |
| TATTTCCTACATAACTTTGT+TGG | 0.375448 | 1.4:+82085924 | None:intergenic |
| TGTAGTAGTGGATGTGAGTC+AGG | 0.391390 | 1.4:-82086427 | MS.gene71605:CDS |
| AGGAGTGGAAGATTTGTCTA+TGG | 0.397750 | 1.4:-82086293 | MS.gene71605:CDS |
| AAATTGTTCTTCCTGGTATG+AGG | 0.406008 | 1.4:+82085761 | None:intergenic |
| TCTTTGTTTAGCAGCTGGTA+TGG | 0.422441 | 1.4:-82085604 | MS.gene71605:intron |
| GGTGGAACTGAAGGGTACTA+TGG | 0.434994 | 1.4:-82086358 | MS.gene71605:CDS |
| GCAGTGTCATGAAGAAAAGA+AGG | 0.507146 | 1.4:+82086118 | None:intergenic |
| GGTACAAAGAAACAGAAAGT+TGG | 0.512053 | 1.4:-82086181 | MS.gene71605:CDS |
| ACAAACCAACAAAGTTATGT+AGG | 0.515352 | 1.4:-82085929 | MS.gene71605:CDS |
| TTGGTTTGTCCCAACAACAT+TGG | 0.519643 | 1.4:+82085943 | None:intergenic |
| ACATCATCATGTGGAAGATG+TGG | 0.558107 | 1.4:+82086247 | None:intergenic |
| AGATTCATAGGTGGAACTGA+AGG | 0.568350 | 1.4:-82086367 | MS.gene71605:CDS |
| GTCACAAATGAGTATTCTGA+AGG | 0.574749 | 1.4:-82086313 | MS.gene71605:CDS |
| TTTGATTCTGGATGTAGTAG+TGG | 0.587821 | 1.4:-82086439 | MS.gene71605:CDS |
| AAATGAGTATTCTGAAGGAG+TGG | 0.595088 | 1.4:-82086308 | MS.gene71605:CDS |
| TTGTTTAGCAGCTGGTATGG+AGG | 0.596105 | 1.4:-82085601 | MS.gene71605:intron |
| GTAGGGAAAATCCTCATACC+AGG | 0.622430 | 1.4:-82085772 | MS.gene71605:intron |
| TCATCATGTGGAAGATGTGG+AGG | 0.636849 | 1.4:+82086250 | None:intergenic |
| TTGAAGTAAACATCATCATG+TGG | 0.637487 | 1.4:+82086238 | None:intergenic |
| GATTCATAGGTGGAACTGAA+GGG | 0.638366 | 1.4:-82086366 | MS.gene71605:CDS |
| GTAGTGGATGTGAGTCAGGT+TGG | 0.642732 | 1.4:-82086423 | MS.gene71605:CDS |
| TGTCATGAAGAAAAGAAGGA+AGG | 0.667075 | 1.4:+82086122 | None:intergenic |
| GAAAACACTAGATTCATAGG+TGG | 0.672436 | 1.4:-82086376 | MS.gene71605:CDS |
| AATATGGTAGACTATTCACA+AGG | 0.724521 | 1.4:-82085905 | MS.gene71605:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATTTGTTTGTGTTATTTGT+AGG | - | chr1.4:82086240-82086259 | MS.gene71605:CDS | 20.0% |
| !!! | ACTAATTATTTTGAGGTAAT+TGG | - | chr1.4:82086158-82086177 | MS.gene71605:CDS | 20.0% |
| !!! | ATTTGTTTGTGTTATTTGTA+GGG | - | chr1.4:82086241-82086260 | MS.gene71605:CDS | 20.0% |
| ! | ACAAAGTTATGTAGGAAATA+TGG | - | chr1.4:82086109-82086128 | MS.gene71605:CDS | 25.0% |
| ! | TATGAAAACACTAGATTCAT+AGG | - | chr1.4:82085651-82085670 | MS.gene71605:intron | 25.0% |
| ! | TATTTCCTACATAACTTTGT+TGG | + | chr1.4:82086109-82086128 | None:intergenic | 25.0% |
| !! | CTTTTCAACTGTAAGAAATA+TGG | - | chr1.4:82085953-82085972 | MS.gene71605:intron | 25.0% |
| !! | TGTTTTCATAAGAATGTTCA+AGG | + | chr1.4:82085641-82085660 | None:intergenic | 25.0% |
| !!! | TTCAGCAACTAATTATTTTG+AGG | - | chr1.4:82086151-82086170 | MS.gene71605:CDS | 25.0% |
| AAAAACCAAATTGTTCTTCC+TGG | + | chr1.4:82086279-82086298 | None:intergenic | 30.0% | |
| AATATGGTAGACTATTCACA+AGG | - | chr1.4:82086125-82086144 | MS.gene71605:CDS | 30.0% | |
| ACAAACCAACAAAGTTATGT+AGG | - | chr1.4:82086101-82086120 | MS.gene71605:CDS | 30.0% | |
| CTCTCAAAAAATGAACTTCA+AGG | - | chr1.4:82086310-82086329 | MS.gene71605:CDS | 30.0% | |
| TTGAAGTAAACATCATCATG+TGG | + | chr1.4:82085795-82085814 | None:intergenic | 30.0% | |
| !! | ATTTTTCAGACCAATGTTGT+TGG | - | chr1.4:82086077-82086096 | MS.gene71605:intron | 30.0% |
| !! | TTTTTCAGACCAATGTTGTT+GGG | - | chr1.4:82086078-82086097 | MS.gene71605:intron | 30.0% |
| !!! | AGTTCATTTTTTGAGAGAGA+TGG | + | chr1.4:82086307-82086326 | None:intergenic | 30.0% |
| AAATTGTTCTTCCTGGTATG+AGG | + | chr1.4:82086272-82086291 | None:intergenic | 35.0% | |
| GAAAACACTAGATTCATAGG+TGG | - | chr1.4:82085654-82085673 | MS.gene71605:intron | 35.0% | |
| GGTACAAAGAAACAGAAAGT+TGG | - | chr1.4:82085849-82085868 | MS.gene71605:intron | 35.0% | |
| GTCACAAATGAGTATTCTGA+AGG | - | chr1.4:82085717-82085736 | MS.gene71605:CDS | 35.0% | |
| TCATACCAGGAAGAACAATT+TGG | - | chr1.4:82086271-82086290 | MS.gene71605:CDS | 35.0% | |
| TGTCATGAAGAAAAGAAGGA+AGG | + | chr1.4:82085911-82085930 | None:intergenic | 35.0% | |
| TTTGATTCTGGATGTAGTAG+TGG | - | chr1.4:82085591-82085610 | MS.gene71605:CDS | 35.0% | |
| ! | AAATGAGTATTCTGAAGGAG+TGG | - | chr1.4:82085722-82085741 | MS.gene71605:CDS | 35.0% |
| ! | GTAGTGAAAAAGCACAAGAA+AGG | - | chr1.4:82085828-82085847 | MS.gene71605:intron | 35.0% |
| ! | TGACTCTTTTGTCTTTGTGT+TGG | + | chr1.4:82085701-82085720 | None:intergenic | 35.0% |
| ! | TTCATTCTTTGTTTAGCAGC+TGG | - | chr1.4:82086421-82086440 | MS.gene71605:CDS | 35.0% |
| ACATCATCATGTGGAAGATG+TGG | + | chr1.4:82085786-82085805 | None:intergenic | 40.0% | |
| AGATTCATAGGTGGAACTGA+AGG | - | chr1.4:82085663-82085682 | MS.gene71605:intron | 40.0% | |
| AGGAGTGGAAGATTTGTCTA+TGG | - | chr1.4:82085737-82085756 | MS.gene71605:CDS | 40.0% | |
| ATGGTTTCTGATGCTTCTTC+TGG | - | chr1.4:82085756-82085775 | MS.gene71605:CDS | 40.0% | |
| GATTCATAGGTGGAACTGAA+GGG | - | chr1.4:82085664-82085683 | MS.gene71605:intron | 40.0% | |
| GCAGTGTCATGAAGAAAAGA+AGG | + | chr1.4:82085915-82085934 | None:intergenic | 40.0% | |
| ! | TCTTTGTTTAGCAGCTGGTA+TGG | - | chr1.4:82086426-82086445 | MS.gene71605:CDS | 40.0% |
| !! | TTGGTTTGTCCCAACAACAT+TGG | + | chr1.4:82086090-82086109 | None:intergenic | 40.0% |
| GTAGGGAAAATCCTCATACC+AGG | - | chr1.4:82086258-82086277 | MS.gene71605:CDS | 45.0% | |
| TCATCATGTGGAAGATGTGG+AGG | + | chr1.4:82085783-82085802 | None:intergenic | 45.0% | |
| TTGTTTAGCAGCTGGTATGG+AGG | - | chr1.4:82086429-82086448 | MS.gene71605:CDS | 45.0% | |
| !! | TGTAGTAGTGGATGTGAGTC+AGG | - | chr1.4:82085603-82085622 | MS.gene71605:intron | 45.0% |
| !! | GGTGGAACTGAAGGGTACTA+TGG | - | chr1.4:82085672-82085691 | MS.gene71605:intron | 50.0% |
| !! | GTAGTGGATGTGAGTCAGGT+TGG | - | chr1.4:82085607-82085626 | MS.gene71605:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 82085582 | 82086470 | 82085582 | ID=MS.gene71605 |
| chr1.4 | mRNA | 82085582 | 82086470 | 82085582 | ID=MS.gene71605.t1;Parent=MS.gene71605 |
| chr1.4 | exon | 82086090 | 82086470 | 82086090 | ID=MS.gene71605.t1.exon1;Parent=MS.gene71605.t1 |
| chr1.4 | CDS | 82086090 | 82086470 | 82086090 | ID=cds.MS.gene71605.t1;Parent=MS.gene71605.t1 |
| chr1.4 | exon | 82085880 | 82085966 | 82085880 | ID=MS.gene71605.t1.exon2;Parent=MS.gene71605.t1 |
| chr1.4 | CDS | 82085880 | 82085966 | 82085880 | ID=cds.MS.gene71605.t1;Parent=MS.gene71605.t1 |
| chr1.4 | exon | 82085714 | 82085790 | 82085714 | ID=MS.gene71605.t1.exon3;Parent=MS.gene71605.t1 |
| chr1.4 | CDS | 82085714 | 82085790 | 82085714 | ID=cds.MS.gene71605.t1;Parent=MS.gene71605.t1 |
| chr1.4 | exon | 82085582 | 82085612 | 82085582 | ID=MS.gene71605.t1.exon4;Parent=MS.gene71605.t1 |
| chr1.4 | CDS | 82085582 | 82085612 | 82085582 | ID=cds.MS.gene71605.t1;Parent=MS.gene71605.t1 |
| Gene Sequence |
| Protein sequence |
