Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73143.t1 | AES70433.2 | 95.9 | 73 | 3 | 0 | 67 | 139 | 148 | 220 | 8.70E-29 | 136.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73143.t1 | Q9SA23 | 75.3 | 73 | 18 | 0 | 67 | 139 | 119 | 191 | 2.1e-23 | 110.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73143.t1 | I3S333 | 95.9 | 73 | 3 | 0 | 67 | 139 | 116 | 188 | 6.3e-29 | 136.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050623 | MS.gene73143 | 0.801191 | 1.01E-48 | -1.69E-46 |
| MS.gene052309 | MS.gene73143 | 0.826076 | 3.31E-54 | -1.69E-46 |
| MS.gene058661 | MS.gene73143 | 0.808325 | 3.27E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73143.t1 | MTR_8g031190 | 94.521 | 73 | 4 | 0 | 67 | 139 | 116 | 188 | 9.71e-43 | 141 |
| MS.gene73143.t1 | MTR_3g055200 | 95.890 | 73 | 3 | 0 | 67 | 139 | 148 | 220 | 2.11e-42 | 141 |
| MS.gene73143.t1 | MTR_3g055230 | 73.846 | 65 | 17 | 0 | 1 | 65 | 963 | 1027 | 2.07e-26 | 103 |
| MS.gene73143.t1 | MTR_3g055230 | 73.438 | 64 | 17 | 0 | 1 | 64 | 963 | 1026 | 7.54e-26 | 102 |
| MS.gene73143.t1 | MTR_3g055230 | 73.438 | 64 | 17 | 0 | 1 | 64 | 963 | 1026 | 7.72e-26 | 102 |
| MS.gene73143.t1 | MTR_3g055230 | 73.438 | 64 | 17 | 0 | 1 | 64 | 963 | 1026 | 7.98e-26 | 102 |
| MS.gene73143.t1 | MTR_3g053180 | 50.769 | 65 | 32 | 0 | 1 | 65 | 956 | 1020 | 2.98e-16 | 75.1 |
| MS.gene73143.t1 | MTR_3g053180 | 50.000 | 64 | 32 | 0 | 1 | 64 | 956 | 1019 | 1.06e-15 | 73.6 |
| MS.gene73143.t1 | MTR_3g053200 | 49.206 | 63 | 32 | 0 | 2 | 64 | 957 | 1019 | 1.86e-14 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73143.t1 | AT1G16240 | 75.342 | 73 | 18 | 0 | 67 | 139 | 119 | 191 | 5.11e-33 | 116 |
| MS.gene73143.t1 | AT1G16240 | 75.342 | 73 | 18 | 0 | 67 | 139 | 119 | 191 | 5.11e-33 | 116 |
| MS.gene73143.t1 | AT1G16240 | 75.342 | 73 | 18 | 0 | 67 | 139 | 119 | 191 | 5.11e-33 | 116 |
| MS.gene73143.t1 | AT1G79590 | 75.342 | 73 | 18 | 0 | 67 | 139 | 120 | 192 | 1.88e-32 | 115 |
| MS.gene73143.t1 | AT1G79590 | 75.342 | 73 | 18 | 0 | 67 | 139 | 120 | 192 | 1.88e-32 | 115 |
| MS.gene73143.t1 | AT1G79590 | 75.342 | 73 | 18 | 0 | 67 | 139 | 151 | 223 | 2.56e-32 | 115 |
| MS.gene73143.t1 | AT1G16240 | 77.465 | 71 | 16 | 0 | 67 | 137 | 119 | 189 | 4.12e-32 | 114 |
| MS.gene73143.t1 | AT1G16230 | 64.286 | 70 | 25 | 0 | 68 | 137 | 118 | 187 | 3.90e-24 | 92.8 |
| MS.gene73143.t1 | AT1G16225 | 52.778 | 72 | 34 | 0 | 68 | 139 | 92 | 163 | 1.35e-15 | 70.9 |
| MS.gene73143.t1 | AT1G16225 | 52.778 | 72 | 34 | 0 | 68 | 139 | 116 | 187 | 2.14e-15 | 70.5 |
Find 26 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATGAGGGCCTTGAGAAAT+TGG | 0.280110 | 3.4:+51153845 | MS.gene73143:CDS |
| CACTTTGCATGATAGTGTTC+GGG | 0.372601 | 3.4:-51153096 | None:intergenic |
| CCACTTTGCATGATAGTGTT+CGG | 0.392671 | 3.4:-51153097 | None:intergenic |
| GGCTCTCTTGACAATCTCAT+TGG | 0.399921 | 3.4:+51153066 | MS.gene73143:CDS |
| GTCCGAAGTTGGCTATGTTA+TGG | 0.405435 | 3.4:+51153157 | MS.gene73143:CDS |
| ATGTAGATGTTACTGATTCT+CGG | 0.418084 | 3.4:+51154051 | MS.gene73143:CDS |
| CTGGAAAACAATGGACTTGT+TGG | 0.446911 | 3.4:+51153712 | MS.gene73143:CDS |
| GCTCTCTTGACAATCTCATT+GGG | 0.465684 | 3.4:+51153067 | MS.gene73143:CDS |
| AGTGGCCATCTCACTCTTTG+AGG | 0.501924 | 3.4:+51153115 | MS.gene73143:CDS |
| AGTTTGAGAACTTGCAATTG+AGG | 0.525561 | 3.4:-51153042 | None:intergenic |
| TGTGAGCATATGTCCGAAGT+TGG | 0.529554 | 3.4:+51153146 | MS.gene73143:CDS |
| ATGTTACTGATTCTCGGCTG+AGG | 0.558353 | 3.4:+51154057 | MS.gene73143:CDS |
| ATGGACTTGTTGGTCTGCAA+CGG | 0.564781 | 3.4:+51153722 | MS.gene73143:CDS |
| TGTTACTGATTCTCGGCTGA+GGG | 0.566984 | 3.4:+51154058 | MS.gene73143:CDS |
| CAATTGCAAGTTCTCAAACT+CGG | 0.568821 | 3.4:+51153045 | MS.gene73143:CDS |
| CGGTCTCCTCCAATTTCTCA+AGG | 0.568939 | 3.4:-51153854 | None:intergenic |
| AATGAGCAGAACTGTTGGCC+TGG | 0.579741 | 3.4:+51153693 | MS.gene73143:CDS |
| TTTCTAACAGAGCAAGATGA+GGG | 0.582199 | 3.4:+51153831 | MS.gene73143:intron |
| GATGCAATGAGCAGAACTGT+TGG | 0.587655 | 3.4:+51153688 | MS.gene73143:intron |
| CTGCAACGGCAAGTTATGAA+AGG | 0.592264 | 3.4:+51153736 | MS.gene73143:CDS |
| ATCCATAACATAGCCAACTT+CGG | 0.592868 | 3.4:-51153159 | None:intergenic |
| TGAGGGCCTTGAGAAATTGG+AGG | 0.594430 | 3.4:+51153848 | MS.gene73143:CDS |
| AAGTACAAAACATATTGCGT+TGG | 0.595524 | 3.4:+51153881 | MS.gene73143:CDS |
| ATCTTCCTCAAAGAGTGAGA+TGG | 0.630729 | 3.4:-51153120 | None:intergenic |
| CCGAACACTATCATGCAAAG+TGG | 0.632855 | 3.4:+51153097 | MS.gene73143:CDS |
| ACTGTTGGCCTGGAAAACAA+TGG | 0.638278 | 3.4:+51153703 | MS.gene73143:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTAAAAAAAATATATAT+TGG | - | chr3.4:51154095-51154114 | None:intergenic | 0.0% |
| !!! | AATATGTTTTGTACTTAATA+CGG | - | chr3.4:51153877-51153896 | None:intergenic | 15.0% |
| !! | AAAGAAATTTAACATAGGAT+GGG | - | chr3.4:51153952-51153971 | None:intergenic | 20.0% |
| !! | TAAATAGAAAAGTAACAACT+AGG | + | chr3.4:51153438-51153457 | MS.gene73143:intron | 20.0% |
| !! | TATTAACATATCTCAATTCA+AGG | - | chr3.4:51154159-51154178 | None:intergenic | 20.0% |
| !! | TCAAGGATTAAAAACTTAAA+GGG | - | chr3.4:51154142-51154161 | None:intergenic | 20.0% |
| !! | TTAGCAAAGAAATTTAACAT+AGG | - | chr3.4:51153957-51153976 | None:intergenic | 20.0% |
| !! | TTCAAGGATTAAAAACTTAA+AGG | - | chr3.4:51154143-51154162 | None:intergenic | 20.0% |
| !!! | TTTGAGATCTATTTTTCTAT+CGG | + | chr3.4:51153980-51153999 | MS.gene73143:intron | 20.0% |
| ! | AAATGTAGTCAGTAAGATAT+TGG | - | chr3.4:51153238-51153257 | None:intergenic | 25.0% |
| ! | ATGAATTCACTATAGTGTAT+CGG | - | chr3.4:51153507-51153526 | None:intergenic | 25.0% |
| ! | ATTAAAAACTTAAAGGGCAA+AGG | - | chr3.4:51154136-51154155 | None:intergenic | 25.0% |
| ! | CAAAGAAATTTAACATAGGA+TGG | - | chr3.4:51153953-51153972 | None:intergenic | 25.0% |
| ! | TAACACAAATTATAGAGCAT+GGG | - | chr3.4:51153273-51153292 | None:intergenic | 25.0% |
| !! | GAAATACATAATTTGTGCTT+TGG | + | chr3.4:51153787-51153806 | MS.gene73143:intron | 25.0% |
| !! | TAATTTGTGTTAGTTACCTT+TGG | + | chr3.4:51153281-51153300 | MS.gene73143:intron | 25.0% |
| AAGTACAAAACATATTGCGT+TGG | + | chr3.4:51153881-51153900 | MS.gene73143:CDS | 30.0% | |
| AATATCCCAACTTTCTTAGA+AGG | + | chr3.4:51153582-51153601 | MS.gene73143:intron | 30.0% | |
| ATATCCCAACTTTCTTAGAA+GGG | + | chr3.4:51153583-51153602 | MS.gene73143:intron | 30.0% | |
| CTAACACAAATTATAGAGCA+TGG | - | chr3.4:51153274-51153293 | None:intergenic | 30.0% | |
| TGAATAGAGAAGATACGTAA+CGG | - | chr3.4:51153659-51153678 | None:intergenic | 30.0% | |
| !! | AGCATTTTAACCATAGTAAC+AGG | - | chr3.4:51153344-51153363 | None:intergenic | 30.0% |
| !! | ATGTAGATGTTACTGATTCT+CGG | + | chr3.4:51154051-51154070 | MS.gene73143:CDS | 30.0% |
| !! | ATTTTCCCTTCTAAGAAAGT+TGG | - | chr3.4:51153591-51153610 | None:intergenic | 30.0% |
| !! | GCATTTTAACCATAGTAACA+GGG | - | chr3.4:51153343-51153362 | None:intergenic | 30.0% |
| !! | TGTGCTTTGGATATTGATTT+CGG | + | chr3.4:51153800-51153819 | MS.gene73143:intron | 30.0% |
| !! | TTTTCCCTTCTAAGAAAGTT+GGG | - | chr3.4:51153590-51153609 | None:intergenic | 30.0% |
| !!! | GATTCTCTTTATTTTCTGCT+AGG | + | chr3.4:51154013-51154032 | MS.gene73143:intron | 30.0% |
| AGTTTGAGAACTTGCAATTG+AGG | - | chr3.4:51153045-51153064 | None:intergenic | 35.0% | |
| ATCCATAACATAGCCAACTT+CGG | - | chr3.4:51153162-51153181 | None:intergenic | 35.0% | |
| CAATTGCAAGTTCTCAAACT+CGG | + | chr3.4:51153045-51153064 | MS.gene73143:CDS | 35.0% | |
| GATATGTGTAATGCATGAGA+AGG | - | chr3.4:51153485-51153504 | None:intergenic | 35.0% | |
| GCATCTTTCAGAATATCCAA+AGG | - | chr3.4:51153300-51153319 | None:intergenic | 35.0% | |
| TAACCATAGTAACAGGGAAA+AGG | - | chr3.4:51153337-51153356 | None:intergenic | 35.0% | |
| TTTCTAACAGAGCAAGATGA+GGG | + | chr3.4:51153831-51153850 | MS.gene73143:intron | 35.0% | |
| ! | TATTTTCTGCTAGGATGACT+TGG | + | chr3.4:51154022-51154041 | MS.gene73143:intron | 35.0% |
| ! | TTTTCTAACAGAGCAAGATG+AGG | + | chr3.4:51153830-51153849 | MS.gene73143:intron | 35.0% |
| ATCTTCCTCAAAGAGTGAGA+TGG | - | chr3.4:51153123-51153142 | None:intergenic | 40.0% | |
| GCTCTCTTGACAATCTCATT+GGG | + | chr3.4:51153067-51153086 | MS.gene73143:CDS | 40.0% | |
| ! | AAGCCTTTTCCCTGTTACTA+TGG | + | chr3.4:51153331-51153350 | MS.gene73143:intron | 40.0% |
| ! | CAACAAGTCCATTGTTTTCC+AGG | - | chr3.4:51153714-51153733 | None:intergenic | 40.0% |
| ! | CACTTTGCATGATAGTGTTC+GGG | - | chr3.4:51153099-51153118 | None:intergenic | 40.0% |
| ! | CCACTTTGCATGATAGTGTT+CGG | - | chr3.4:51153100-51153119 | None:intergenic | 40.0% |
| ! | CTGGAAAACAATGGACTTGT+TGG | + | chr3.4:51153712-51153731 | MS.gene73143:CDS | 40.0% |
| !! | ATGCGAAGAGTGCTTAATTG+TGG | - | chr3.4:51153368-51153387 | None:intergenic | 40.0% |
| !! | GCTTTGGATATTGATTTCGG+AGG | + | chr3.4:51153803-51153822 | MS.gene73143:intron | 40.0% |
| ACAGTTCTGCTCATTGCATC+TGG | - | chr3.4:51153688-51153707 | None:intergenic | 45.0% | |
| AGATGAGGGCCTTGAGAAAT+TGG | + | chr3.4:51153845-51153864 | MS.gene73143:CDS | 45.0% | |
| ATAGAGCATGGGAAGCAGAA+TGG | - | chr3.4:51153262-51153281 | None:intergenic | 45.0% | |
| CCGAACACTATCATGCAAAG+TGG | + | chr3.4:51153097-51153116 | MS.gene73143:CDS | 45.0% | |
| CTGCAACGGCAAGTTATGAA+AGG | + | chr3.4:51153736-51153755 | MS.gene73143:CDS | 45.0% | |
| GATGCAATGAGCAGAACTGT+TGG | + | chr3.4:51153688-51153707 | MS.gene73143:intron | 45.0% | |
| GGCTCTCTTGACAATCTCAT+TGG | + | chr3.4:51153066-51153085 | MS.gene73143:CDS | 45.0% | |
| GTCCGAAGTTGGCTATGTTA+TGG | + | chr3.4:51153157-51153176 | MS.gene73143:CDS | 45.0% | |
| TGTGAGCATATGTCCGAAGT+TGG | + | chr3.4:51153146-51153165 | MS.gene73143:CDS | 45.0% | |
| ! | ACTGTTGGCCTGGAAAACAA+TGG | + | chr3.4:51153703-51153722 | MS.gene73143:CDS | 45.0% |
| !! | ATGGACTTGTTGGTCTGCAA+CGG | + | chr3.4:51153722-51153741 | MS.gene73143:CDS | 45.0% |
| !! | ATGTTACTGATTCTCGGCTG+AGG | + | chr3.4:51154057-51154076 | MS.gene73143:CDS | 45.0% |
| !! | TGTTACTGATTCTCGGCTGA+GGG | + | chr3.4:51154058-51154077 | MS.gene73143:CDS | 45.0% |
| AATGAGCAGAACTGTTGGCC+TGG | + | chr3.4:51153693-51153712 | MS.gene73143:CDS | 50.0% | |
| AGTGGCCATCTCACTCTTTG+AGG | + | chr3.4:51153115-51153134 | MS.gene73143:CDS | 50.0% | |
| CGGTCTCCTCCAATTTCTCA+AGG | - | chr3.4:51153857-51153876 | None:intergenic | 50.0% | |
| TGAGGGCCTTGAGAAATTGG+AGG | + | chr3.4:51153848-51153867 | MS.gene73143:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 51153036 | 51154226 | 51153036 | ID=MS.gene73143 |
| chr3.4 | mRNA | 51153036 | 51154226 | 51153036 | ID=MS.gene73143.t1;Parent=MS.gene73143 |
| chr3.4 | exon | 51153036 | 51153231 | 51153036 | ID=MS.gene73143.t1.exon1;Parent=MS.gene73143.t1 |
| chr3.4 | CDS | 51153036 | 51153231 | 51153036 | ID=cds.MS.gene73143.t1;Parent=MS.gene73143.t1 |
| chr3.4 | exon | 51153689 | 51153757 | 51153689 | ID=MS.gene73143.t1.exon2;Parent=MS.gene73143.t1 |
| chr3.4 | CDS | 51153689 | 51153757 | 51153689 | ID=cds.MS.gene73143.t1;Parent=MS.gene73143.t1 |
| chr3.4 | exon | 51153841 | 51153941 | 51153841 | ID=MS.gene73143.t1.exon3;Parent=MS.gene73143.t1 |
| chr3.4 | CDS | 51153841 | 51153941 | 51153841 | ID=cds.MS.gene73143.t1;Parent=MS.gene73143.t1 |
| chr3.4 | exon | 51154035 | 51154079 | 51154035 | ID=MS.gene73143.t1.exon4;Parent=MS.gene73143.t1 |
| chr3.4 | CDS | 51154035 | 51154079 | 51154035 | ID=cds.MS.gene73143.t1;Parent=MS.gene73143.t1 |
| chr3.4 | exon | 51154185 | 51154226 | 51154185 | ID=MS.gene73143.t1.exon5;Parent=MS.gene73143.t1 |
| chr3.4 | CDS | 51154185 | 51154226 | 51154185 | ID=cds.MS.gene73143.t1;Parent=MS.gene73143.t1 |
| Gene Sequence |
| Protein sequence |
