Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73246.t1 | XP_013470091.1 | 97.2 | 36 | 1 | 0 | 1 | 36 | 41 | 76 | 2.00E-11 | 77.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73246.t1 | F4I4K5 | 58.3 | 36 | 15 | 0 | 1 | 36 | 42 | 77 | 6.3e-06 | 50.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73246.t1 | A0A072VQ29 | 97.2 | 36 | 1 | 0 | 1 | 36 | 41 | 76 | 1.4e-11 | 77.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05954 | MS.gene73246 | 0.800178 | 1.63E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73246.t1 | MTR_1g107555 | 97.222 | 36 | 1 | 0 | 1 | 36 | 41 | 76 | 6.53e-19 | 78.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATATACTTTGGGCATATT+AGG | 0.261148 | 4.1:+78664509 | None:intergenic |
| CCTAATATGCCCAAAGTATA+TGG | 0.293487 | 4.1:-78664509 | MS.gene73246:CDS |
| AATCTTGAGACACTTTGATT+TGG | 0.303412 | 4.1:+78664463 | None:intergenic |
| ATCTTGAGACACTTTGATTT+GGG | 0.361134 | 4.1:+78664464 | None:intergenic |
| TTGGCATGTTCAAGAAGAAA+TGG | 0.363926 | 4.1:-78664442 | MS.gene73246:CDS |
| AACCTTGTAGCCATATACTT+TGG | 0.384612 | 4.1:+78664499 | None:intergenic |
| ACCTTGTAGCCATATACTTT+GGG | 0.412818 | 4.1:+78664500 | None:intergenic |
| TATGGCTACAAGGTTGTAGA+TGG | 0.445406 | 4.1:-78664491 | MS.gene73246:CDS |
| AAATCAAAGTGTCTCAAGAT+TGG | 0.450223 | 4.1:-78664461 | MS.gene73246:CDS |
| GATTCACATGAGGAGGCACT+AGG | 0.510736 | 4.1:-78663659 | None:intergenic |
| TGGCATGTTCAAGAAGAAAT+GGG | 0.517657 | 4.1:-78664441 | MS.gene73246:intron |
| TAAAATATCCTCAAAATCAA+AGG | 0.538229 | 4.1:-78664232 | MS.gene73246:CDS |
| ATTCACATGAGGAGGCACTA+GGG | 0.560459 | 4.1:-78663658 | None:intergenic |
| GCCCAAAGTATATGGCTACA+AGG | 0.574012 | 4.1:-78664501 | MS.gene73246:CDS |
| TATTGAGAGGGATTCACATG+AGG | 0.589603 | 4.1:-78663669 | MS.gene73246:CDS |
| ATACTTTGGGCATATTAGGA+AGG | 0.591992 | 4.1:+78664513 | None:intergenic |
| TTCACATGAGGAGGCACTAG+GGG | 0.594984 | 4.1:-78663657 | None:intergenic |
| TGAGAGGGATTCACATGAGG+AGG | 0.596847 | 4.1:-78663666 | MS.gene73246:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTAAAATATTATTATTTTGC+AGG | - | chr4.1:78664476-78664495 | MS.gene73246:CDS | 15.0% |
| !! | AAACAAGTAAAGAATGAATT+TGG | + | chr4.1:78663846-78663865 | None:intergenic | 20.0% |
| !! | AATAAAGACAACAAAATAAG+AGG | + | chr4.1:78663815-78663834 | None:intergenic | 20.0% |
| !! | AATATTCATCAAGTTCAATA+AGG | + | chr4.1:78664008-78664027 | None:intergenic | 20.0% |
| !! | TAAAATATCCTCAAAATCAA+AGG | - | chr4.1:78663955-78663974 | MS.gene73246:intron | 20.0% |
| !!! | ATACACTGATGTTATTTTTA+TGG | - | chr4.1:78664129-78664148 | MS.gene73246:intron | 20.0% |
| ! | AGTAAAGAATGAATTTGGAA+TGG | + | chr4.1:78663841-78663860 | None:intergenic | 25.0% |
| ! | TGAAAAAAGGATTATTGAGA+GGG | - | chr4.1:78664506-78664525 | MS.gene73246:CDS | 25.0% |
| ! | TTGAAAAAAGGATTATTGAG+AGG | - | chr4.1:78664505-78664524 | MS.gene73246:CDS | 25.0% |
| !! | AGTATAATAATGTTGCTTGT+GGG | - | chr4.1:78664364-78664383 | MS.gene73246:intron | 25.0% |
| !! | TGCTTCAGTTTTTGTAATAT+AGG | - | chr4.1:78664327-78664346 | MS.gene73246:intron | 25.0% |
| AATCTTGAGACACTTTGATT+TGG | + | chr4.1:78663727-78663746 | None:intergenic | 30.0% | |
| CATTCTTTACTTGTTTCGAT+AGG | - | chr4.1:78663850-78663869 | MS.gene73246:intron | 30.0% | |
| GTTCATTTATGCTAGTTGTT+GGG | - | chr4.1:78664098-78664117 | MS.gene73246:intron | 30.0% | |
| TGTTCATTTATGCTAGTTGT+TGG | - | chr4.1:78664097-78664116 | MS.gene73246:intron | 30.0% | |
| ! | AAATCAAAGTGTCTCAAGAT+TGG | - | chr4.1:78663726-78663745 | MS.gene73246:intron | 30.0% |
| ! | ATCTTGAGACACTTTGATTT+GGG | + | chr4.1:78663726-78663745 | None:intergenic | 30.0% |
| ! | CAGTATAATAATGTTGCTTG+TGG | - | chr4.1:78664363-78664382 | MS.gene73246:intron | 30.0% |
| !! | AGCAATATCCTTTGATTTTG+AGG | + | chr4.1:78663966-78663985 | None:intergenic | 30.0% |
| !!! | CACTGATGTTATTTTTATGG+AGG | - | chr4.1:78664132-78664151 | MS.gene73246:intron | 30.0% |
| AACCTTGTAGCCATATACTT+TGG | + | chr4.1:78663691-78663710 | None:intergenic | 35.0% | |
| ACCTTGTAGCCATATACTTT+GGG | + | chr4.1:78663690-78663709 | None:intergenic | 35.0% | |
| ATACTTTGGGCATATTAGGA+AGG | + | chr4.1:78663677-78663696 | None:intergenic | 35.0% | |
| CCATATACTTTGGGCATATT+AGG | + | chr4.1:78663681-78663700 | None:intergenic | 35.0% | |
| CCTAATATGCCCAAAGTATA+TGG | - | chr4.1:78663678-78663697 | MS.gene73246:CDS | 35.0% | |
| TACCACAGTTCTTGAATTTC+AGG | - | chr4.1:78663877-78663896 | MS.gene73246:intron | 35.0% | |
| TGCAGGATGAGTTTGAAAAA+AGG | - | chr4.1:78664493-78664512 | MS.gene73246:CDS | 35.0% | |
| TGGCATGTTCAAGAAGAAAT+GGG | - | chr4.1:78663746-78663765 | MS.gene73246:intron | 35.0% | |
| TTGGCATGTTCAAGAAGAAA+TGG | - | chr4.1:78663745-78663764 | MS.gene73246:intron | 35.0% | |
| ! | ACAAAAACTGAAGCATCAAG+TGG | + | chr4.1:78664322-78664341 | None:intergenic | 35.0% |
| CTCCTGAAATTCAAGAACTG+TGG | + | chr4.1:78663882-78663901 | None:intergenic | 40.0% | |
| GAGGATATTCGCATGTAATG+AGG | + | chr4.1:78663796-78663815 | None:intergenic | 40.0% | |
| TATGGCTACAAGGTTGTAGA+TGG | - | chr4.1:78663696-78663715 | MS.gene73246:intron | 40.0% | |
| TATTGAGAGGGATTCACATG+AGG | - | chr4.1:78664518-78664537 | MS.gene73246:CDS | 40.0% | |
| ! | AAGTGGAGTTAGGATTGGTA+TGG | + | chr4.1:78664305-78664324 | None:intergenic | 40.0% |
| CAGTGCTCAGTCTAAGTCAT+AGG | + | chr4.1:78664256-78664275 | None:intergenic | 45.0% | |
| GCATCAAGTGGAGTTAGGAT+TGG | + | chr4.1:78664310-78664329 | None:intergenic | 45.0% | |
| GCCCAAAGTATATGGCTACA+AGG | - | chr4.1:78663686-78663705 | MS.gene73246:CDS | 45.0% | |
| ! | CTGAAGCATCAAGTGGAGTT+AGG | + | chr4.1:78664315-78664334 | None:intergenic | 45.0% |
| TGAGAGGGATTCACATGAGG+AGG | - | chr4.1:78664521-78664540 | MS.gene73246:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 78663660 | 78664549 | 78663660 | ID=MS.gene73246 |
| chr4.1 | mRNA | 78663660 | 78664549 | 78663660 | ID=MS.gene73246.t1;Parent=MS.gene73246 |
| chr4.1 | exon | 78664442 | 78664549 | 78664442 | ID=MS.gene73246.t1.exon1;Parent=MS.gene73246.t1 |
| chr4.1 | CDS | 78664442 | 78664549 | 78664442 | ID=cds.MS.gene73246.t1;Parent=MS.gene73246.t1 |
| chr4.1 | exon | 78664224 | 78664255 | 78664224 | ID=MS.gene73246.t1.exon2;Parent=MS.gene73246.t1 |
| chr4.1 | CDS | 78664224 | 78664255 | 78664224 | ID=cds.MS.gene73246.t1;Parent=MS.gene73246.t1 |
| chr4.1 | exon | 78663660 | 78663711 | 78663660 | ID=MS.gene73246.t1.exon3;Parent=MS.gene73246.t1 |
| chr4.1 | CDS | 78663660 | 78663711 | 78663660 | ID=cds.MS.gene73246.t1;Parent=MS.gene73246.t1 |
| Gene Sequence |
| Protein sequence |
