Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73328.t1 | XP_013454613.1 | 48.2 | 369 | 16 | 4 | 1 | 195 | 97 | 464 | 6.10E-67 | 263.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73328.t1 | P56725 | 36.0 | 369 | 61 | 5 | 1 | 195 | 87 | 454 | 9.6e-45 | 181.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73328.t1 | A0A072UFW6 | 48.2 | 369 | 16 | 4 | 1 | 195 | 97 | 464 | 4.4e-67 | 263.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053836 | MS.gene73328 | 0.80462 | 1.98E-49 | -1.69E-46 |
| MS.gene056723 | MS.gene73328 | 0.803191 | 3.92E-49 | -1.69E-46 |
| MS.gene057121 | MS.gene73328 | 0.808961 | 2.39E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73328.t1 | MTR_4g008630 | 57.848 | 223 | 13 | 3 | 1 | 143 | 97 | 318 | 2.03e-72 | 226 |
| MS.gene73328.t1 | MTR_4g008630 | 94.231 | 52 | 3 | 0 | 144 | 195 | 413 | 464 | 3.12e-25 | 101 |
| MS.gene73328.t1 | MTR_1g029540 | 52.232 | 224 | 25 | 3 | 1 | 143 | 93 | 315 | 1.17e-61 | 198 |
| MS.gene73328.t1 | MTR_1g029540 | 82.692 | 52 | 9 | 0 | 144 | 195 | 410 | 461 | 3.15e-22 | 93.2 |
| MS.gene73328.t1 | MTR_8g090095 | 37.295 | 244 | 62 | 5 | 1 | 162 | 109 | 343 | 3.38e-36 | 132 |
| MS.gene73328.t1 | MTR_8g090095 | 80.392 | 51 | 10 | 0 | 145 | 195 | 431 | 481 | 1.34e-20 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TCAGTTATATATGTATCTTT+TGG | - | 11114:2056-2075 | MS.gene73328:CDS | 20.0% |
| ! | AAAGCAAGATAAAGTTCATT+TGG | - | 11114:2135-2154 | MS.gene73328:intron | 25.0% |
| ! | AAGCAAGATAAAGTTCATTT+GGG | - | 11114:2136-2155 | MS.gene73328:intron | 25.0% |
| ! | AATTCTATGCATTAAGTGAT+GGG | - | 11114:1858-1877 | MS.gene73328:intron | 25.0% |
| ! | ACATATTTGATGGAAATGAA+GGG | - | 11114:2183-2202 | MS.gene73328:intron | 25.0% |
| ! | ATAACACAAGTGTTAAAGAT+TGG | - | 11114:2422-2441 | MS.gene73328:CDS | 25.0% |
| ! | ATATTGAGAATGTTGTTAGA+AGG | - | 11114:2495-2514 | MS.gene73328:intron | 25.0% |
| ! | GATACATATATAACTGAGTT+TGG | + | 11114:2053-2072 | MS.gene73328:intergenic | 25.0% |
| ! | TATTATTGATGTTCCTTCTA+TGG | - | 11114:1784-1803 | MS.gene73328:intron | 25.0% |
| !! | ACTTTCATCTGTTTTCAAAA+GGG | - | 11114:1608-1627 | MS.gene73328:CDS | 25.0% |
| !! | TACAATTTTCACTTCCATTT+GGG | - | 11114:1727-1746 | MS.gene73328:intron | 25.0% |
| !! | TTACAATTTTCACTTCCATT+TGG | - | 11114:1726-1745 | MS.gene73328:intron | 25.0% |
| !!! | ATTAAAGAAGTCACTGATTT+CGG | + | 11114:1828-1847 | MS.gene73328:intergenic | 25.0% |
| AAATTGAACAGATTGCAACT+GGG | - | 11114:2105-2124 | MS.gene73328:intron | 30.0% | |
| ATAATATGCGAGCTTTCAAT+CGG | + | 11114:1770-1789 | MS.gene73328:intergenic | 30.0% | |
| GAATTCTATGCATTAAGTGA+TGG | - | 11114:1857-1876 | MS.gene73328:intron | 30.0% | |
| GACATATTTGATGGAAATGA+AGG | - | 11114:2182-2201 | MS.gene73328:intron | 30.0% | |
| GATAAAGGAGACATATTTGA+TGG | - | 11114:2173-2192 | MS.gene73328:intron | 30.0% | |
| GTTATGAAAACACAGTTTCT+TGG | + | 11114:2407-2426 | MS.gene73328:intergenic | 30.0% | |
| TGTGATTCATGATAGTCTAA+TGG | - | 11114:1635-1654 | MS.gene73328:CDS | 30.0% | |
| TTCAATTTCTTCATCTGTCA+AGG | + | 11114:2093-2112 | MS.gene73328:intergenic | 30.0% | |
| TTTGATCTTTCTCTTCTCAA+TGG | + | 11114:1990-2009 | MS.gene73328:intergenic | 30.0% | |
| ! | ACAAGTGTTAAAGATTGGTT+TGG | - | 11114:2427-2446 | MS.gene73328:CDS | 30.0% |
| ! | CACTTTCATCTGTTTTCAAA+AGG | - | 11114:1607-1626 | MS.gene73328:CDS | 30.0% |
| !! | ACTTTAGAGAGCATTTCAAT+GGG | - | 11114:2344-2363 | MS.gene73328:CDS | 30.0% |
| !! | TACTTTAGAGAGCATTTCAA+TGG | - | 11114:2343-2362 | MS.gene73328:CDS | 30.0% |
| AAAAGAGGTGAACTTGCAAA+AGG | - | 11114:2206-2225 | MS.gene73328:intron | 35.0% | |
| AAAGAGGTGAACTTGCAAAA+GGG | - | 11114:2207-2226 | MS.gene73328:intron | 35.0% | |
| AAAGAGTAGGCATTCATGTA+TGG | - | 11114:2007-2026 | MS.gene73328:CDS | 35.0% | |
| AAATGCTATTGCTATGTCGA+TGG | - | 11114:2571-2590 | MS.gene73328:CDS | 35.0% | |
| AGTCATTGAAAGTCCTTACT+TGG | - | 11114:1901-1920 | MS.gene73328:intron | 35.0% | |
| GAAATTGAACAGATTGCAAC+TGG | - | 11114:2104-2123 | MS.gene73328:intron | 35.0% | |
| GAAGAGAAAGATCAAAGAGT+AGG | - | 11114:1994-2013 | MS.gene73328:CDS | 35.0% | |
| GAATGTTGTTAGAAGGTTGA+TGG | - | 11114:2502-2521 | MS.gene73328:intron | 35.0% | |
| TATTGATGTTCCTTCTATGG+AGG | - | 11114:1787-1806 | MS.gene73328:intron | 35.0% | |
| TTCTCTTCTCAATGGTAAGA+GGG | + | 11114:1982-2001 | MS.gene73328:intergenic | 35.0% | |
| TTTCTCTTCTCAATGGTAAG+AGG | + | 11114:1983-2002 | MS.gene73328:intergenic | 35.0% | |
| !! | AAAGATTGGTTTGGTTGTGA+TGG | - | 11114:2436-2455 | MS.gene73328:intron | 35.0% |
| !! | CTTTAGAGAGCATTTCAATG+GGG | - | 11114:2345-2364 | MS.gene73328:CDS | 35.0% |
| !! | TAGAAGGTTGATGGATACAA+AGG | - | 11114:2511-2530 | MS.gene73328:intron | 35.0% |
| !! | TTTAGAGAGCATTTCAATGG+GGG | - | 11114:2346-2365 | MS.gene73328:CDS | 35.0% |
| AAGAGAGAGTTGAAGGAATG+GGG | - | 11114:2234-2253 | MS.gene73328:intron | 40.0% | |
| ACTTCCATTTGGGAGAAAGT+GGG | - | 11114:1737-1756 | MS.gene73328:intron | 40.0% | |
| ATTGCTATGTCGATGGATGA+AGG | - | 11114:2578-2597 | MS.gene73328:CDS | 40.0% | |
| CTCTCTATCAACTCCAAGTA+AGG | + | 11114:1917-1936 | MS.gene73328:intergenic | 40.0% | |
| GAAGAGAGAGTTGAAGGAAT+GGG | - | 11114:2233-2252 | MS.gene73328:intron | 40.0% | |
| GATGGAAATGAAGGGAAAAG+AGG | - | 11114:2191-2210 | MS.gene73328:intron | 40.0% | |
| GGTTTATGAGTCATTGTGGA+TGG | - | 11114:2315-2334 | MS.gene73328:CDS | 40.0% | |
| GTAGGCATTCATGTATGGAA+TGG | - | 11114:2012-2031 | MS.gene73328:CDS | 40.0% | |
| GTGGCTTAGAATTTCCAACT+GGG | + | 11114:2282-2301 | MS.gene73328:intergenic | 40.0% | |
| GTGTTGAGAGATGCTGATAA+AGG | - | 11114:2158-2177 | MS.gene73328:intron | 40.0% | |
| TCAATGGTAAGAGGGTTGAA+GGG | + | 11114:1974-1993 | MS.gene73328:intergenic | 40.0% | |
| TCATAAACCCTCCTGTTGAA+GGG | + | 11114:2304-2323 | MS.gene73328:intergenic | 40.0% | |
| TGAAAACACAGTTTCTTGGC+TGG | + | 11114:2403-2422 | MS.gene73328:intergenic | 40.0% | |
| TGAAGAGAGAGTTGAAGGAA+TGG | - | 11114:2232-2251 | MS.gene73328:intron | 40.0% | |
| TGATAGTCTAATGGCATCAG+TGG | - | 11114:1644-1663 | MS.gene73328:CDS | 40.0% | |
| TGGCTTAGAATTTCCAACTG+GGG | + | 11114:2281-2300 | MS.gene73328:intergenic | 40.0% | |
| TGTGAAGAGGCTAGAAAAGA+AGG | + | 11114:1555-1574 | MS.gene73328:intergenic | 40.0% | |
| TTGGGCTAAAAGACATGAGT+TGG | - | 11114:2460-2479 | MS.gene73328:intron | 40.0% | |
| ! | TCACTTGCAGTAAGAAGCAT+TGG | - | 11114:1939-1958 | MS.gene73328:intron | 40.0% |
| ! | TTTTCCCACTTTCTCCCAAA+TGG | + | 11114:1744-1763 | MS.gene73328:intergenic | 40.0% |
| !! | AGGTTGATGGATACAAAGGA+AGG | - | 11114:2515-2534 | MS.gene73328:intron | 40.0% |
| !! | CACTTGCAGTAAGAAGCATT+GGG | - | 11114:1940-1959 | MS.gene73328:intron | 40.0% |
| !! | TGGTTTGGTTGTGATGGATT+GGG | - | 11114:2442-2461 | MS.gene73328:intron | 40.0% |
| !! | TTGGTTTGGTTGTGATGGAT+TGG | - | 11114:2441-2460 | MS.gene73328:intron | 40.0% |
| AAGCATCCATCCTCCATAGA+AGG | + | 11114:1800-1819 | MS.gene73328:intergenic | 45.0% | |
| ATATGCGAGCTTTCAATCGG+AGG | + | 11114:1767-1786 | MS.gene73328:intergenic | 45.0% | |
| ATTCTAAGCCACCCTTCAAC+AGG | - | 11114:2290-2309 | MS.gene73328:CDS | 45.0% | |
| CACTTCCATTTGGGAGAAAG+TGG | - | 11114:1736-1755 | MS.gene73328:intron | 45.0% | |
| CGGAGTTTCTCGTATGGAAA+TGG | - | 11114:2601-2620 | MS.gene73328:CDS | 45.0% | |
| CTCAATGGTAAGAGGGTTGA+AGG | + | 11114:1975-1994 | MS.gene73328:intergenic | 45.0% | |
| CTCATAAACCCTCCTGTTGA+AGG | + | 11114:2305-2324 | MS.gene73328:intergenic | 45.0% | |
| GATGTTCCTTCTATGGAGGA+TGG | - | 11114:1791-1810 | MS.gene73328:intron | 45.0% | |
| GATTGCATGAGTTCTACCAC+TGG | + | 11114:1588-1607 | MS.gene73328:intergenic | 45.0% | |
| GGAGGGTTTATGAGTCATTG+TGG | - | 11114:2311-2330 | MS.gene73328:CDS | 45.0% | |
| GGGTTTGAAGAGAGAGTTGA+AGG | - | 11114:2227-2246 | MS.gene73328:intron | 45.0% | |
| GGTGGCTTAGAATTTCCAAC+TGG | + | 11114:2283-2302 | MS.gene73328:intergenic | 45.0% | |
| TGAACAGATTGCAACTGGGT+TGG | - | 11114:2109-2128 | MS.gene73328:intron | 45.0% | |
| !! | AGTAAGAAGCATTGGGCAGT+GGG | - | 11114:1947-1966 | MS.gene73328:intron | 45.0% |
| !! | GGGGTTTGTTGTGAGAGATT+GGG | - | 11114:2253-2272 | MS.gene73328:CDS | 45.0% |
| !! | TGGGGTTTGTTGTGAGAGAT+TGG | - | 11114:2252-2271 | MS.gene73328:CDS | 45.0% |
| GCTATGTCGATGGATGAAGG+CGG | - | 11114:2581-2600 | MS.gene73328:CDS | 50.0% | |
| TAAACCCTCCTGTTGAAGGG+TGG | + | 11114:2301-2320 | MS.gene73328:intergenic | 50.0% | |
| TAAGCCACCCTTCAACAGGA+GGG | - | 11114:2294-2313 | MS.gene73328:CDS | 50.0% | |
| TGAAGGCGGAGTTTCTCGTA+TGG | - | 11114:2595-2614 | MS.gene73328:CDS | 50.0% | |
| TGCATAGGCCATGCTGCTAT+TGG | + | 11114:2374-2393 | MS.gene73328:intergenic | 50.0% | |
| TGGCTCACGAAGATGTGAAG+AGG | + | 11114:1568-1587 | MS.gene73328:intergenic | 50.0% | |
| TTCACATCTTCGTGAGCCAG+TGG | - | 11114:1569-1588 | MS.gene73328:CDS | 50.0% | |
| ! | CTTGGCTGGTCAGAATGCAT+AGG | + | 11114:2389-2408 | MS.gene73328:intergenic | 50.0% |
| !! | CAGTAAGAAGCATTGGGCAG+TGG | - | 11114:1946-1965 | MS.gene73328:intron | 50.0% |
| CTAAGCCACCCTTCAACAGG+AGG | - | 11114:2293-2312 | MS.gene73328:CDS | 55.0% | |
| GAGAGATTGGGCACCCCAGT+TGG | - | 11114:2265-2284 | MS.gene73328:CDS | 60.0% | |
| !! | TGGGGGTGCCAATAGCAGCA+TGG | - | 11114:2363-2382 | MS.gene73328:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11114 | gene | 1546 | 2652 | 1546 | ID=MS.gene73328 |
| 11114 | mRNA | 1546 | 2652 | 1546 | ID=MS.gene73328.t1;Parent=MS.gene73328 |
| 11114 | exon | 2533 | 2652 | 2533 | ID=MS.gene73328.t1.exon1;Parent=MS.gene73328.t1 |
| 11114 | CDS | 2533 | 2652 | 2533 | ID=cds.MS.gene73328.t1;Parent=MS.gene73328.t1 |
| 11114 | exon | 2251 | 2446 | 2251 | ID=MS.gene73328.t1.exon2;Parent=MS.gene73328.t1 |
| 11114 | CDS | 2251 | 2446 | 2251 | ID=cds.MS.gene73328.t1;Parent=MS.gene73328.t1 |
| 11114 | exon | 1986 | 2099 | 1986 | ID=MS.gene73328.t1.exon3;Parent=MS.gene73328.t1 |
| 11114 | CDS | 1986 | 2099 | 1986 | ID=cds.MS.gene73328.t1;Parent=MS.gene73328.t1 |
| 11114 | exon | 1546 | 1703 | 1546 | ID=MS.gene73328.t1.exon4;Parent=MS.gene73328.t1 |
| 11114 | CDS | 1546 | 1703 | 1546 | ID=cds.MS.gene73328.t1;Parent=MS.gene73328.t1 |
| Gene Sequence |
| Protein sequence |
