AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73752


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73752.t1 MTR_7g082540 76.494 251 58 1 1 251 1 250 6.00e-137 386
MS.gene73752.t1 MTR_7g082390 78.008 241 52 1 7 247 10 249 9.65e-134 378
MS.gene73752.t1 MTR_7g082533 36.017 236 133 6 32 251 36 269 4.33e-43 148
MS.gene73752.t1 MTR_7g082537 31.076 251 148 10 2 238 7 246 2.75e-29 111
MS.gene73752.t1 MTR_6g082940 30.000 260 159 9 1 239 1 258 8.37e-29 112
MS.gene73752.t1 MTR_6g082920 30.000 260 159 9 1 239 1 258 8.37e-29 112
MS.gene73752.t1 MTR_6g082820 28.571 252 152 9 10 239 13 258 5.71e-28 108
MS.gene73752.t1 MTR_6g083000 29.811 265 160 10 1 242 1 262 2.59e-27 108
MS.gene73752.t1 MTR_7g082530 33.188 229 143 7 27 246 31 258 1.45e-26 108
MS.gene73752.t1 MTR_7g082530 33.188 229 143 7 27 246 31 258 1.60e-26 108
MS.gene73752.t1 MTR_7g082460 28.352 261 157 9 14 253 21 272 2.52e-26 107
MS.gene73752.t1 MTR_8g104520 32.143 252 147 10 1 236 1 244 1.36e-24 102
MS.gene73752.t1 MTR_7g082470 27.143 280 141 10 16 257 1 255 9.35e-22 94.4
MS.gene73752.t1 MTR_6g082980 29.018 224 136 9 1 206 5 223 2.36e-21 90.5
MS.gene73752.t1 MTR_6g082990 29.386 228 149 7 38 256 35 259 7.94e-20 86.3
MS.gene73752.t1 MTR_6g083010 28.826 281 164 15 3 256 15 286 1.27e-18 83.6
MS.gene73752.t1 MTR_1g115485 27.068 266 166 10 13 255 7 267 1.40e-18 85.1
MS.gene73752.t1 MTR_7g082510 28.452 239 133 10 14 238 21 235 2.56e-17 81.6
MS.gene73752.t1 MTR_7g082430 28.169 213 112 8 32 238 41 218 2.70e-17 81.3
MS.gene73752.t1 MTR_6g044830 31.343 201 122 8 36 223 4 201 8.17e-17 80.1
MS.gene73752.t1 MTR_6g083760 28.241 216 139 10 36 243 44 251 4.01e-15 75.1
MS.gene73752.t1 MTR_6g082870 28.926 242 136 11 33 247 51 283 4.18e-14 72.0
MS.gene73752.t1 MTR_6g083780 28.191 188 121 6 36 216 52 232 2.45e-13 69.7
MS.gene73752.t1 MTR_6g083020 25.359 209 145 7 38 240 47 250 3.88e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73752.t1 AT1G66880 36.139 202 115 4 32 230 33 223 2.02e-31 122
MS.gene73752.t1 AT1G66880 36.139 202 115 4 32 230 33 223 3.86e-31 122
MS.gene73752.t1 AT1G66880 26.549 226 146 7 26 235 246 467 6.68e-20 89.4
MS.gene73752.t1 AT1G67000 36.404 228 117 8 27 246 26 233 6.81e-31 121
MS.gene73752.t1 AT1G67000 28.512 242 142 11 22 241 241 473 2.78e-21 93.2
MS.gene73752.t1 AT1G66940 34.182 275 126 10 11 246 9 267 8.60e-31 117
MS.gene73752.t1 AT1G66940 34.182 275 126 10 11 246 9 267 1.55e-30 117
MS.gene73752.t1 AT1G66940 34.340 265 120 10 11 238 9 256 1.82e-30 115
MS.gene73752.t1 AT1G66940 34.340 265 120 10 11 238 9 256 1.82e-30 115
MS.gene73752.t1 AT1G66930 35.644 202 116 5 32 230 33 223 3.20e-30 119
MS.gene73752.t1 AT1G66930 28.700 223 139 6 32 238 252 470 1.19e-18 85.5
MS.gene73752.t1 AT1G66880 35.859 198 113 4 36 230 416 602 1.01e-29 117
MS.gene73752.t1 AT1G66880 31.250 192 122 5 38 228 26 208 3.14e-21 93.2
MS.gene73752.t1 AT1G66880 26.549 226 146 7 26 235 625 846 8.98e-20 89.0
MS.gene73752.t1 AT1G66880 27.717 184 110 7 51 226 229 397 1.76e-11 64.3
MS.gene73752.t1 AT1G66920 35.652 230 130 8 32 256 33 249 1.38e-28 114
MS.gene73752.t1 AT1G66920 35.652 230 130 8 32 256 33 249 1.56e-28 114
MS.gene73752.t1 AT1G66920 37.129 202 110 7 32 228 33 222 8.99e-28 112
MS.gene73752.t1 AT5G38260 30.453 243 153 7 11 239 12 252 2.26e-26 107
MS.gene73752.t1 AT5G38210 31.602 231 139 7 3 229 14 229 1.73e-25 105
MS.gene73752.t1 AT5G38210 27.727 220 141 7 32 239 20 233 2.83e-23 99.0
MS.gene73752.t1 AT5G38210 27.354 223 144 7 29 239 35 251 3.27e-23 99.0
MS.gene73752.t1 AT1G66910 25.600 250 158 6 14 239 17 262 1.01e-18 85.5
MS.gene73752.t1 AT1G66910 26.400 250 156 8 14 239 17 262 1.14e-18 85.5
MS.gene73752.t1 AT5G38250 30.964 197 116 6 36 225 41 224 1.99e-18 84.7
MS.gene73752.t1 AT5G38240 34.536 194 103 8 23 205 26 206 1.16e-16 79.7
MS.gene73752.t1 AT5G38240 34.536 194 103 8 23 205 26 206 1.19e-16 79.7
MS.gene73752.t1 AT1G66930 28.037 214 134 6 32 229 71 280 1.72e-15 76.3
MS.gene73752.t1 AT1G66930 28.037 214 134 6 32 229 71 280 1.95e-15 75.9
MS.gene73752.t1 AT1G67025 27.363 201 134 8 44 239 17 210 1.60e-14 70.9
MS.gene73752.t1 AT1G18390 24.766 214 143 7 46 251 50 253 4.92e-12 65.9

Find 47 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGGTTTGGTTCTTATAT+TGG 0.250167 7.3:+25316622 MS.gene73752:CDS
AACACTGACCTCTTTGCTTA+TGG 0.260209 7.3:+25316484 MS.gene73752:CDS
TGTGGAACTATAAAACAAAT+TGG 0.275223 7.3:+25316256 MS.gene73752:CDS
AATAATCTTACACTCTTCTA+TGG 0.285737 7.3:+25316517 MS.gene73752:CDS
TCCTTGCAAATTGTCCTCTT+TGG 0.289570 7.3:-25316428 None:intergenic
GAATATGTTTATACTTGGTT+TGG 0.334018 7.3:+25316610 MS.gene73752:CDS
AAAGGGTGGCCACACTCTTT+TGG 0.336772 7.3:-25316304 None:intergenic
TCATTTGAGCTCAAATATAA+AGG 0.368635 7.3:-25316211 None:intergenic
CTGGAACAACCATACTTTGT+TGG 0.394164 7.3:-25316663 None:intergenic
TGTGAGAAGTGTAAAGAATC+AGG 0.395896 7.3:+25316781 MS.gene73752:CDS
TACTTTCCATGTCCTTCAAA+TGG 0.402968 7.3:+25316577 MS.gene73752:CDS
GTGGTTATAACTGGACTTCA+AGG 0.407568 7.3:+25316812 MS.gene73752:CDS
GTGTTTAGTGTTGTGTTGAC+AGG 0.432638 7.3:-25316466 None:intergenic
TCAAAAGGATATCCATTTGA+AGG 0.455340 7.3:-25316589 None:intergenic
TGCAATTGAGATTCAACAAA+GGG 0.455829 7.3:-25316321 None:intergenic
ATACTTTGTTGGCATGAAAA+CGG 0.485354 7.3:-25316652 None:intergenic
CAAGTATAAACATATTCAAA+AGG 0.511931 7.3:-25316604 None:intergenic
GGATATCCATTTGAAGGACA+TGG 0.511967 7.3:-25316583 None:intergenic
AGACTGATCTCTGCAGTAGC+AGG 0.524963 7.3:-25316842 None:intergenic
CAAAGTATGTCCACCAGGGT+TGG 0.531376 7.3:-25316398 None:intergenic
TGTGAGAATCTGTTTAATTG+TGG 0.537109 7.3:+25316238 MS.gene73752:CDS
TTATATTTGAGCTCAAATGA+AGG 0.537398 7.3:+25316214 MS.gene73752:CDS
GGTCGATCCCCTCCCCAGAA+AGG 0.542122 7.3:-25316283 None:intergenic
TTTGTGGTGAGATGGATAGC+TGG 0.564260 7.3:+25316748 MS.gene73752:CDS
GGAAAGTATCCACGTGGCGA+AGG 0.565003 7.3:-25316562 None:intergenic
TGCAATCAGAGATGGATTTG+TGG 0.566962 7.3:+25316732 MS.gene73752:CDS
ATACAAAGTGCAATCAGAGA+TGG 0.568148 7.3:+25316724 MS.gene73752:CDS
TGTTCTAATGCACAGTCTCA+AGG 0.575655 7.3:+25316865 MS.gene73752:CDS
GAGATGGATTTGTGGTGAGA+TGG 0.580019 7.3:+25316740 MS.gene73752:CDS
ATTCTCAAAGTATGTCCACC+AGG 0.580295 7.3:-25316403 None:intergenic
GGCGAAGGTAAAGTAAATGA+AGG 0.586984 7.3:-25316547 None:intergenic
TGAAGGATAATAAACAAGGA+GGG 0.609659 7.3:-25316148 None:intergenic
GTGCAATTGAGATTCAACAA+AGG 0.612374 7.3:-25316322 None:intergenic
GTATCTGAATCAGTTTCAAG+AGG 0.620450 7.3:-25316189 None:intergenic
AGTCAGAACCATAAGCAAAG+AGG 0.620955 7.3:-25316492 None:intergenic
TACTTTGAGAATCACCAAAG+AGG 0.625009 7.3:+25316414 MS.gene73752:CDS
ACCAAAGAGGACAATTTGCA+AGG 0.627376 7.3:+25316427 MS.gene73752:CDS
ATGAAGGATAATAAACAAGG+AGG 0.646970 7.3:-25316149 None:intergenic
TTATGACTTGAAGAACATGA+AGG 0.662073 7.3:-25316165 None:intergenic
GGGGATCGACCAAAAGAGTG+TGG 0.667307 7.3:+25316295 MS.gene73752:CDS
AATTGAGATTCAACAAAGGG+TGG 0.667350 7.3:-25316318 None:intergenic
GAGAAGTGTAAAGAATCAGG+TGG 0.667414 7.3:+25316784 MS.gene73752:CDS
AACATGAAGGATAATAAACA+AGG 0.673826 7.3:-25316152 None:intergenic
TTTACTTTACCTTCGCCACG+TGG 0.674813 7.3:+25316553 MS.gene73752:CDS
GAAGGTAAAGTAAATGAAGG+AGG 0.677333 7.3:-25316544 None:intergenic
TTCTCAAAGTATGTCCACCA+GGG 0.712267 7.3:-25316402 None:intergenic
GGACATGGAAAGTATCCACG+TGG 0.769460 7.3:-25316568 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAGTATAAACATATTCAAA+AGG - chr7.3:25316607-25316626 None:intergenic 20.0%
!!! CTTTTGAATATGTTTATACT+TGG + chr7.3:25316605-25316624 MS.gene73752:CDS 20.0%
! AACATGAAGGATAATAAACA+AGG - chr7.3:25316155-25316174 None:intergenic 25.0%
! AATAATCTTACACTCTTCTA+TGG + chr7.3:25316517-25316536 MS.gene73752:CDS 25.0%
! GAATATGTTTATACTTGGTT+TGG + chr7.3:25316610-25316629 MS.gene73752:CDS 25.0%
! TCAAAAAATGAATCAGAAAC+TGG - chr7.3:25316685-25316704 None:intergenic 25.0%
! TCATTTGAGCTCAAATATAA+AGG - chr7.3:25316214-25316233 None:intergenic 25.0%
! TGTGGAACTATAAAACAAAT+TGG + chr7.3:25316256-25316275 MS.gene73752:CDS 25.0%
! TTATATTTGAGCTCAAATGA+AGG + chr7.3:25316214-25316233 MS.gene73752:CDS 25.0%
ATACTTTGTTGGCATGAAAA+CGG - chr7.3:25316655-25316674 None:intergenic 30.0%
ATGAAGGATAATAAACAAGG+AGG - chr7.3:25316152-25316171 None:intergenic 30.0%
TAGTATGAACAACAATTGCA+AGG + chr7.3:25316948-25316967 MS.gene73752:intron 30.0%
TCAAAAGGATATCCATTTGA+AGG - chr7.3:25316592-25316611 None:intergenic 30.0%
TGAAGGATAATAAACAAGGA+GGG - chr7.3:25316151-25316170 None:intergenic 30.0%
TGCAATTGAGATTCAACAAA+GGG - chr7.3:25316324-25316343 None:intergenic 30.0%
TGTGAGAATCTGTTTAATTG+TGG + chr7.3:25316238-25316257 MS.gene73752:CDS 30.0%
TTATGACTTGAAGAACATGA+AGG - chr7.3:25316168-25316187 None:intergenic 30.0%
!! ACTTGGTTTGGTTCTTATAT+TGG + chr7.3:25316622-25316641 MS.gene73752:CDS 30.0%
!!! AACAAATTGGTTTTCCTTTC+TGG + chr7.3:25316269-25316288 MS.gene73752:CDS 30.0%
!!! ACAAATTGGTTTTCCTTTCT+GGG + chr7.3:25316270-25316289 MS.gene73752:CDS 30.0%
AATTGAGATTCAACAAAGGG+TGG - chr7.3:25316321-25316340 None:intergenic 35.0%
ATACAAAGTGCAATCAGAGA+TGG + chr7.3:25316724-25316743 MS.gene73752:CDS 35.0%
GAAGGTAAAGTAAATGAAGG+AGG - chr7.3:25316547-25316566 None:intergenic 35.0%
GAGAAAAACACAACACTTAG+CGG + chr7.3:25316972-25316991 MS.gene73752:CDS 35.0%
GTATCTGAATCAGTTTCAAG+AGG - chr7.3:25316192-25316211 None:intergenic 35.0%
GTGCAATTGAGATTCAACAA+AGG - chr7.3:25316325-25316344 None:intergenic 35.0%
TACTTTCCATGTCCTTCAAA+TGG + chr7.3:25316577-25316596 MS.gene73752:CDS 35.0%
! GTGGATTTTGTGGTTATAAC+TGG + chr7.3:25316803-25316822 MS.gene73752:CDS 35.0%
! GTTTTCATGCCAACAAAGTA+TGG + chr7.3:25316654-25316673 MS.gene73752:CDS 35.0%
! TACTTTGAGAATCACCAAAG+AGG + chr7.3:25316414-25316433 MS.gene73752:CDS 35.0%
! TGTGAGAAGTGTAAAGAATC+AGG + chr7.3:25316781-25316800 MS.gene73752:CDS 35.0%
!! AAAGAATCAGGTGGATTTTG+TGG + chr7.3:25316793-25316812 MS.gene73752:CDS 35.0%
!!! CAAATTGGTTTTCCTTTCTG+GGG + chr7.3:25316271-25316290 MS.gene73752:CDS 35.0%
AACACTGACCTCTTTGCTTA+TGG + chr7.3:25316484-25316503 MS.gene73752:CDS 40.0%
ACCAAAGAGGACAATTTGCA+AGG + chr7.3:25316427-25316446 MS.gene73752:CDS 40.0%
AGTCAGAACCATAAGCAAAG+AGG - chr7.3:25316495-25316514 None:intergenic 40.0%
ATTCTCAAAGTATGTCCACC+AGG - chr7.3:25316406-25316425 None:intergenic 40.0%
CTGGAACAACCATACTTTGT+TGG - chr7.3:25316666-25316685 None:intergenic 40.0%
GGATATCCATTTGAAGGACA+TGG - chr7.3:25316586-25316605 None:intergenic 40.0%
GGCGAAGGTAAAGTAAATGA+AGG - chr7.3:25316550-25316569 None:intergenic 40.0%
GTGGTTATAACTGGACTTCA+AGG + chr7.3:25316812-25316831 MS.gene73752:CDS 40.0%
TCCTTGCAAATTGTCCTCTT+TGG - chr7.3:25316431-25316450 None:intergenic 40.0%
TGTTCTAATGCACAGTCTCA+AGG + chr7.3:25316865-25316884 MS.gene73752:CDS 40.0%
TTCTCAAAGTATGTCCACCA+GGG - chr7.3:25316405-25316424 None:intergenic 40.0%
! GAGAAGTGTAAAGAATCAGG+TGG + chr7.3:25316784-25316803 MS.gene73752:CDS 40.0%
! GTGTTTAGTGTTGTGTTGAC+AGG - chr7.3:25316469-25316488 None:intergenic 40.0%
! TGCAATCAGAGATGGATTTG+TGG + chr7.3:25316732-25316751 MS.gene73752:CDS 40.0%
!! GTTGTGTTGACAGGTTTTGT+TGG - chr7.3:25316460-25316479 None:intergenic 40.0%
TTTACTTTACCTTCGCCACG+TGG + chr7.3:25316553-25316572 MS.gene73752:CDS 45.0%
TTTGTGGTGAGATGGATAGC+TGG + chr7.3:25316748-25316767 MS.gene73752:CDS 45.0%
! GAGATGGATTTGTGGTGAGA+TGG + chr7.3:25316740-25316759 MS.gene73752:CDS 45.0%
!! TTGGTTTTCCTTTCTGGGGA+GGG + chr7.3:25316275-25316294 MS.gene73752:CDS 45.0%
!!! ATTGGTTTTCCTTTCTGGGG+AGG + chr7.3:25316274-25316293 MS.gene73752:CDS 45.0%
!!! CATGTTTTAGAAGCCAACCC+TGG + chr7.3:25316385-25316404 MS.gene73752:CDS 45.0%
AGACTGATCTCTGCAGTAGC+AGG - chr7.3:25316845-25316864 None:intergenic 50.0%
CAAAGTATGTCCACCAGGGT+TGG - chr7.3:25316401-25316420 None:intergenic 50.0%
! AAAGGGTGGCCACACTCTTT+TGG - chr7.3:25316307-25316326 None:intergenic 50.0%
!! GGACATGGAAAGTATCCACG+TGG - chr7.3:25316571-25316590 None:intergenic 50.0%
!! TGGTTTTCCTTTCTGGGGAG+GGG + chr7.3:25316276-25316295 MS.gene73752:CDS 50.0%
!!! GTTTTAGAAGCCAACCCTGG+TGG + chr7.3:25316388-25316407 MS.gene73752:CDS 50.0%
GGGGATCGACCAAAAGAGTG+TGG + chr7.3:25316295-25316314 MS.gene73752:CDS 55.0%
!! GGAAAGTATCCACGTGGCGA+AGG - chr7.3:25316565-25316584 None:intergenic 55.0%
GGTCGATCCCCTCCCCAGAA+AGG - chr7.3:25316286-25316305 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 25316145 25317001 25316145 ID=MS.gene73752
chr7.3 mRNA 25316145 25317001 25316145 ID=MS.gene73752.t1;Parent=MS.gene73752
chr7.3 exon 25316145 25316886 25316145 ID=MS.gene73752.t1.exon1;Parent=MS.gene73752.t1
chr7.3 CDS 25316145 25316886 25316145 ID=cds.MS.gene73752.t1;Parent=MS.gene73752.t1
chr7.3 exon 25316970 25317001 25316970 ID=MS.gene73752.t1.exon2;Parent=MS.gene73752.t1
chr7.3 CDS 25316970 25317001 25316970 ID=cds.MS.gene73752.t1;Parent=MS.gene73752.t1
Gene Sequence

>MS.gene73752

ATGCCCTCCTTGTTTATTATCCTTCATGTTCTTCAAGTCATAATCCTCTTGAAACTGATTCAGATACCTTTATATTTGAGCTCAAATGAAGGTTGTGAGAATCTGTTTAATTGTGGAACTATAAAACAAATTGGTTTTCCTTTCTGGGGAGGGGATCGACCAAAAGAGTGTGGCCACCCTTTGTTGAATCTCAATTGCACAAACAACATGAGCTACATAACAATCAATGAAGTCAAGTATCATGTTTTAGAAGCCAACCCTGGTGGACATACTTTGAGAATCACCAAAGAGGACAATTTGCAAGGACTATGTCCAACAAAACCTGTCAACACAACACTAAACACTGACCTCTTTGCTTATGGTTCTGACTACAATAATCTTACACTCTTCTATGGTTGTCCTCCTTCATTTACTTTACCTTCGCCACGTGGATACTTTCCATGTCCTTCAAATGGATATCCTTTTGAATATGTTTATACTTGGTTTGGTTCTTATATTGGTCTACAACCGTTTTCATGCCAACAAAGTATGGTTGTTCCAGTTTCTGATTCATTTTTTGATGTTCATGATTTGACTAAAATACAAAGTGCAATCAGAGATGGATTTGTGGTGAGATGGATAGCTGGTATTGAAGATTGTGAGAAGTGTAAAGAATCAGGTGGATTTTGTGGTTATAACTGGACTTCAAGGAAAACAACCTGCTACTGCAGAGATCAGTCTTGTTCTAATGCACAGTCTCAAGGTATGTCTAAAGATTTTTGCTAATTGCTAATTGCATTTCTATTCTCTATAAAATGATGATATAGTATGAACAACAATTGCAAGGTGAGAAAAACACAACACTTAGCGGAAAATAA

Protein sequence

>MS.gene73752.t1

MPSLFIILHVLQVIILLKLIQIPLYLSSNEGCENLFNCGTIKQIGFPFWGGDRPKECGHPLLNLNCTNNMSYITINEVKYHVLEANPGGHTLRITKEDNLQGLCPTKPVNTTLNTDLFAYGSDYNNLTLFYGCPPSFTLPSPRGYFPCPSNGYPFEYVYTWFGSYIGLQPFSCQQSMVVPVSDSFFDVHDLTKIQSAIRDGFVVRWIAGIEDCEKCKESGGFCGYNWTSRKTTCYCRDQSCSNAQSQGEKNTTLSGK