AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene73872

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73872.t1 MTR_4g077797 89.377 273 15 2 2 267 77 342 2.39e-177 492
MS.gene73872.t1 MTR_4g077803 80.882 272 34 4 3 267 84 344 4.80e-158 443
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene73872.t1 AT5G03415 62.416 298 60 9 12 267 98 385 8.46e-114 332
MS.gene73872.t1 AT5G03415 60.067 298 61 10 12 267 98 379 6.87e-106 312
MS.gene73872.t1 AT5G02470 51.759 199 81 2 14 204 50 241 1.64e-69 216
MS.gene73872.t1 AT5G02470 51.759 199 81 2 14 204 50 241 1.64e-69 216
MS.gene73872.t1 AT5G02470 51.759 199 81 2 14 204 50 241 1.64e-69 216

Find 57 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTCAAGGCAATGGATTT+TGG 0.207282 4.4:+32725352 MS.gene73872:CDS
TCCACTCCCTGGAATATTAA+AGG 0.271571 4.4:+32725513 MS.gene73872:CDS
TATTATTGCTACAAGTTTGT+AGG 0.286595 4.4:+32724364 MS.gene73872:intron
ATTGTTCATTTCGTTGAATA+AGG 0.299922 4.4:-32724396 None:intergenic
TATACCTGAACCAGAATAAA+AGG 0.312486 4.4:-32724460 None:intergenic
ACTCTTGCCTTTAATATTCC+AGG 0.333887 4.4:-32725520 None:intergenic
TGATGCTCTGAATGTTCTTA+TGG 0.333958 4.4:+32723946 MS.gene73872:CDS
AACCTTTCATACTAAATTGC+CGG 0.339281 4.4:-32722729 None:intergenic
CAGTTTCAAACATGTTAGTT+AGG 0.354238 4.4:+32725476 MS.gene73872:CDS
TTAAATAGACACAGCGTCTT+GGG 0.420475 4.4:+32724146 MS.gene73872:intron
GCCTTTAATATTCCAGGGAG+TGG 0.432840 4.4:-32725514 None:intergenic
AGACACAGCGTCTTGGGCTT+AGG 0.448668 4.4:+32724152 MS.gene73872:intron
TCCTCATGCAACTGTGGAAG+TGG 0.457569 4.4:+32724984 MS.gene73872:CDS
ATTAAATAGACACAGCGTCT+TGG 0.463317 4.4:+32724145 MS.gene73872:intron
GGGGAAGGTTCTAGCATGTC+AGG 0.468504 4.4:+32725427 MS.gene73872:CDS
GGCTATGAATATTATTTCCA+AGG 0.477616 4.4:+32723967 MS.gene73872:CDS
AGGTAAAGATACACCTCCAC+TGG 0.479385 4.4:-32724440 None:intergenic
CTGCTCAAGCTCCTGCAGAT+AGG 0.480130 4.4:-32724197 None:intergenic
GAGTGATATTGAAGAACTAA+AGG 0.483152 4.4:+32724033 MS.gene73872:CDS
CTCCGGCAATTTAGTATGAA+AGG 0.485877 4.4:+32722727 MS.gene73872:CDS
AATGAACAATTATATAGCTC+AGG 0.501710 4.4:+32724409 MS.gene73872:CDS
ATAAGAATGGAAGAGGGCTC+CGG 0.512718 4.4:+32722710 MS.gene73872:CDS
CTCTTGCCTTTAATATTCCA+GGG 0.515553 4.4:-32725519 None:intergenic
TGTGAGAAGGTAGAAAGTAA+AGG 0.516897 4.4:+32723239 MS.gene73872:CDS
GGCTGTTGGGGTGATAAGAA+TGG 0.521904 4.4:+32722697 MS.gene73872:CDS
CAATTATGTTCTCAAGGCAA+TGG 0.525211 4.4:+32725345 MS.gene73872:CDS
CCGACATCACCTCCACTCCC+TGG 0.527073 4.4:+32725502 MS.gene73872:CDS
GTCAGGCATGTATCAATCAC+AGG 0.555543 4.4:+32725444 MS.gene73872:CDS
TGGGGTGATAAGAATGGAAG+AGG 0.559374 4.4:+32722703 MS.gene73872:CDS
TTGTCGTCATGCAGCTCGAA+AGG 0.559999 4.4:-32725325 None:intergenic
CACACAATATTGCGGATGGA+GGG 0.564114 4.4:+32725407 MS.gene73872:CDS
GGCTTAGGAGTAGAATTGAA+AGG 0.565800 4.4:+32724167 MS.gene73872:CDS
TTTAATATTCCAGGGAGTGG+AGG 0.566833 4.4:-32725511 None:intergenic
AAGGAAAGCAGCCTATCTGC+AGG 0.574961 4.4:+32724186 MS.gene73872:CDS
AATATTGCGGATGGAGGGGA+AGG 0.575401 4.4:+32725412 MS.gene73872:CDS
TTCTTCAATATCACTCAGAC+TGG 0.575701 4.4:-32724026 None:intergenic
CCAGGGAGTGGAGGTGATGT+CGG 0.580031 4.4:-32725502 None:intergenic
ACACAATATTGCGGATGGAG+GGG 0.585147 4.4:+32725408 MS.gene73872:CDS
AGAACAACTTACATGAGGTT+AGG 0.595613 4.4:+32723263 MS.gene73872:CDS
ACACACAATATTGCGGATGG+AGG 0.602906 4.4:+32725406 MS.gene73872:CDS
TGACGACAATTATGTTCTCA+AGG 0.607641 4.4:+32725339 MS.gene73872:CDS
TCCACTTCCACAGTTGCATG+AGG 0.608745 4.4:-32724985 None:intergenic
GTTACACACAATATTGCGGA+TGG 0.615879 4.4:+32725403 MS.gene73872:CDS
GGGAGTGGAGGTGATGTCGG+AGG 0.628434 4.4:-32725499 None:intergenic
CAATGTTACACACAATATTG+CGG 0.641067 4.4:+32725399 MS.gene73872:CDS
TCAGGAAATCCTCCCAGTGG+AGG 0.644133 4.4:+32724427 MS.gene73872:CDS
GACACGTCCTCATGCAACTG+TGG 0.649899 4.4:+32724978 MS.gene73872:intron
TGTGTAACATTGTCACTCTG+TGG 0.651492 4.4:-32725388 None:intergenic
AAGGAAGAACAACTTACATG+AGG 0.678207 4.4:+32723258 MS.gene73872:CDS
GAGCATCATAGACCCTTCGA+CGG 0.678223 4.4:-32723931 None:intergenic
GGTAAAGATACACCTCCACT+GGG 0.679973 4.4:-32724439 None:intergenic
AGCTCAGGAAATCCTCCCAG+TGG 0.682103 4.4:+32724424 MS.gene73872:CDS
ATAGGTGGCAGATGAACTTG+TGG 0.689893 4.4:+32723738 MS.gene73872:intron
GGGGTGATAAGAATGGAAGA+GGG 0.692220 4.4:+32722704 MS.gene73872:CDS
AACATGTTTGAAACTGACTG+AGG 0.695881 4.4:-32725469 None:intergenic
AAAGATACACCTCCACTGGG+AGG 0.724160 4.4:-32724436 None:intergenic
ATATCACTCAGACTGGTACG+AGG 0.734989 4.4:-32724019 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAAATTTTTTTTTT+AGG - chr4.4:32723723-32723704 None:intergenic 0.0%
!!! TTTTTTTTTTTTTTAAATAT+AGG + chr4.4:32723720-32723739 MS.gene73872:intron 0.0%
!!! AATTTTTTTTTTAGGAAATA+AGG - chr4.4:32723696-32723715 None:intergenic 10.0%
!!! TTTTTTTTTTTAAATATAGG+TGG + chr4.4:32723723-32723742 MS.gene73872:intron 10.0%
!!! ATCTTATGAACTTATTTTTT+TGG + chr4.4:32723867-32723886 MS.gene73872:intron 15.0%
!!! TCTTATGAACTTATTTTTTT+GGG + chr4.4:32723868-32723887 MS.gene73872:intron 15.0%
!! AAAAAAGAAATACAATGGAA+GGG + chr4.4:32723992-32724011 MS.gene73872:CDS 20.0%
!! AATTTAAAAAATAGCAATCC+AGG - chr4.4:32724269-32724288 None:intergenic 20.0%
!! AGGATAAAAAAGAAATACAA+TGG + chr4.4:32723987-32724006 MS.gene73872:CDS 20.0%
!! ATGTAATATATTATCATCCA+AGG - chr4.4:32724548-32724567 None:intergenic 20.0%
!! CCTATTAAAAAACTAATTGA+AGG - chr4.4:32723507-32723526 None:intergenic 20.0%
!! TAAAAAAGAAATACAATGGA+AGG + chr4.4:32723991-32724010 MS.gene73872:CDS 20.0%
!! TAAAAAGTTAAAGATCAAAG+TGG - chr4.4:32722774-32722793 None:intergenic 20.0%
!! TTCTATAAATACATACATGT+TGG + chr4.4:32723429-32723448 MS.gene73872:intron 20.0%
!!! AAAAAACTGGTTTAAAGTAA+AGG - chr4.4:32723289-32723308 None:intergenic 20.0%
!!! AGAAATAATTTTCCCTTTTT+TGG + chr4.4:32724697-32724716 MS.gene73872:intron 20.0%
!!! CCTTCAATTAGTTTTTTAAT+AGG + chr4.4:32723504-32723523 MS.gene73872:intron 20.0%
!!! GAAATAATTTTCCCTTTTTT+GGG + chr4.4:32724698-32724717 MS.gene73872:intron 20.0%
!!! TTATTTGCTATTACACATTA+AGG + chr4.4:32723834-32723853 MS.gene73872:intron 20.0%
!!! TTGTATTTCTTTTTTATCCT+TGG - chr4.4:32723987-32724006 None:intergenic 20.0%
!!! TTGTTCATTTTGATTTCAAT+AGG + chr4.4:32725028-32725047 MS.gene73872:CDS 20.0%
! AAATCAACAGTAAATTAGAG+AGG - chr4.4:32723210-32723229 None:intergenic 25.0%
! AATCAACAGTAAATTAGAGA+GGG - chr4.4:32723209-32723228 None:intergenic 25.0%
! AATGAACAATTATATAGCTC+AGG + chr4.4:32724409-32724428 MS.gene73872:CDS 25.0%
! AATTGAACAACAATTTCTAC+TGG - chr4.4:32724522-32724541 None:intergenic 25.0%
! AGAGAGTAAACATAAATAAG+TGG - chr4.4:32725295-32725314 None:intergenic 25.0%
! ATAACATAGACCCATAAAAT+AGG - chr4.4:32724815-32724834 None:intergenic 25.0%
! ATTACATGAACTTAAGTAGT+TGG + chr4.4:32724592-32724611 MS.gene73872:intron 25.0%
! ATTGTTCATTTCGTTGAATA+AGG - chr4.4:32724399-32724418 None:intergenic 25.0%
! CTTTATGATACCACAAAATT+AGG - chr4.4:32725139-32725158 None:intergenic 25.0%
! GATAAATCACAACATGAAAT+GGG - chr4.4:32723629-32723648 None:intergenic 25.0%
! GGTATTAAATCAATTAGAGT+GGG - chr4.4:32724236-32724255 None:intergenic 25.0%
! GTAGAGAAAATTGTCAAAAA+TGG - chr4.4:32723171-32723190 None:intergenic 25.0%
! TAGTATATAGCAGTAACTTT+CGG - chr4.4:32724745-32724764 None:intergenic 25.0%
! TATTATTGCTACAAGTTTGT+AGG + chr4.4:32724364-32724383 MS.gene73872:intron 25.0%
! TGATAAATCACAACATGAAA+TGG - chr4.4:32723630-32723649 None:intergenic 25.0%
!! AAGACTTTATATTTAGAACG+TGG - chr4.4:32723388-32723407 None:intergenic 25.0%
!! ATTTTATGGGTCTATGTTAT+GGG + chr4.4:32724815-32724834 MS.gene73872:intron 25.0%
!! CAAATAAGTTGTTTCAAACA+AGG - chr4.4:32722834-32722853 None:intergenic 25.0%
!! CACTCTAATTGATTTAATAC+CGG + chr4.4:32724235-32724254 MS.gene73872:intron 25.0%
!! TATTTTATGGGTCTATGTTA+TGG + chr4.4:32724814-32724833 MS.gene73872:intron 25.0%
!! TGTATCTTTACCTTTTATTC+TGG + chr4.4:32724450-32724469 MS.gene73872:CDS 25.0%
!!! ATTTTCAAGTAGATAAGTCT+TGG + chr4.4:32725216-32725235 MS.gene73872:intron 25.0%
!!! GACAGAGATTTATTTTATTC+AGG + chr4.4:32723145-32723164 MS.gene73872:intron 25.0%
!!! TGACAATTTTCTCTACTTTT+TGG + chr4.4:32723175-32723194 MS.gene73872:intron 25.0%
!!! TTTTCAAGTAGATAAGTCTT+GGG + chr4.4:32725217-32725236 MS.gene73872:intron 25.0%
AACCTTTCATACTAAATTGC+CGG - chr4.4:32722732-32722751 None:intergenic 30.0%
AAGATAAGAAAACCTGATCA+GGG - chr4.4:32723797-32723816 None:intergenic 30.0%
AATCAAATATCAGCACCAAT+AGG - chr4.4:32724934-32724953 None:intergenic 30.0%
AGGAAGTCTAAACATTAAGA+CGG - chr4.4:32725119-32725138 None:intergenic 30.0%
AGTTACTGCTATATACTAGA+TGG + chr4.4:32724747-32724766 MS.gene73872:intron 30.0%
ATCAACAGTAAATTAGAGAG+GGG - chr4.4:32723208-32723227 None:intergenic 30.0%
CAATGTTACACACAATATTG+CGG + chr4.4:32725399-32725418 MS.gene73872:CDS 30.0%
CAGTTTCAAACATGTTAGTT+AGG + chr4.4:32725476-32725495 MS.gene73872:CDS 30.0%
CGGTATTAAATCAATTAGAG+TGG - chr4.4:32724237-32724256 None:intergenic 30.0%
GAGTGATATTGAAGAACTAA+AGG + chr4.4:32724033-32724052 MS.gene73872:CDS 30.0%
GGCTATGAATATTATTTCCA+AGG + chr4.4:32723967-32723986 MS.gene73872:CDS 30.0%
GTCTATGTTATGGGTTTATA+CGG + chr4.4:32724824-32724843 MS.gene73872:intron 30.0%
TAAACATGTGTGTAAAGGTT+TGG + chr4.4:32722865-32722884 MS.gene73872:intron 30.0%
TATACCTGAACCAGAATAAA+AGG - chr4.4:32724463-32724482 None:intergenic 30.0%
TGAATAATATGCTCATCTGT+TGG + chr4.4:32724892-32724911 MS.gene73872:intron 30.0%
TGACATAAACATGTGTGTAA+AGG + chr4.4:32722860-32722879 MS.gene73872:intron 30.0%
! ATTGTTGTTCAATTATGCCT+TGG + chr4.4:32724528-32724547 MS.gene73872:intron 30.0%
! CAGATAAATTTTCCAACACA+GGG - chr4.4:32725272-32725291 None:intergenic 30.0%
! CTTTTAACGTGGTAAAAAAC+TGG - chr4.4:32723302-32723321 None:intergenic 30.0%
! GTTTAGACTTCCTAATTTTG+TGG + chr4.4:32725126-32725145 MS.gene73872:intron 30.0%
! GTTTTGTCAATGATTAAGCA+CGG + chr4.4:32723526-32723545 MS.gene73872:intron 30.0%
! TAATTATTAGCCTCAGAAAC+TGG + chr4.4:32723577-32723596 MS.gene73872:intron 30.0%
! TGAATTTTCTGATCAAGCAA+TGG + chr4.4:32723762-32723781 MS.gene73872:CDS 30.0%
!! GTTATGATGTGATTAGCTTT+AGG + chr4.4:32725085-32725104 MS.gene73872:intron 30.0%
!!! CAATTTTCTCTACTTTTTGG+TGG + chr4.4:32723178-32723197 MS.gene73872:intron 30.0%
!!! TTTACCTTTTATTCTGGTTC+AGG + chr4.4:32724456-32724475 MS.gene73872:CDS 30.0%
AACTCAGATAATGACAAGCA+AGG - chr4.4:32725068-32725087 None:intergenic 35.0%
AAGGAAGAACAACTTACATG+AGG + chr4.4:32723258-32723277 MS.gene73872:CDS 35.0%
AATCACAACATGAAATGGGT+AGG - chr4.4:32723625-32723644 None:intergenic 35.0%
ACTCTTGCCTTTAATATTCC+AGG - chr4.4:32725523-32725542 None:intergenic 35.0%
ACTGGACAAAATGACCAAAA+AGG - chr4.4:32724504-32724523 None:intergenic 35.0%
AGAACAACTTACATGAGGTT+AGG + chr4.4:32723263-32723282 MS.gene73872:CDS 35.0%
AGAGTTTATGCATAAGCTCT+TGG + chr4.4:32722996-32723015 MS.gene73872:intron 35.0%
AGATAAGAAAACCTGATCAG+GGG - chr4.4:32723796-32723815 None:intergenic 35.0%
ATGAACTTAAGTAGTTGGCT+TGG + chr4.4:32724597-32724616 MS.gene73872:intron 35.0%
ATTAAATAGACACAGCGTCT+TGG + chr4.4:32724145-32724164 MS.gene73872:intron 35.0%
CAATTATGTTCTCAAGGCAA+TGG + chr4.4:32725345-32725364 MS.gene73872:CDS 35.0%
CCAAACAAATGCCCAAAAAA+GGG - chr4.4:32724712-32724731 None:intergenic 35.0%
CTCAATCATGGATAGCAAAT+CGG + chr4.4:32723329-32723348 MS.gene73872:intron 35.0%
CTCTTGCCTTTAATATTCCA+GGG - chr4.4:32725522-32725541 None:intergenic 35.0%
GAAAATAGATCTTATCCAGC+TGG + chr4.4:32724659-32724678 MS.gene73872:intron 35.0%
GAAACTAGTGTATCCTATTG+TGG + chr4.4:32725170-32725189 MS.gene73872:intron 35.0%
GAAGATAAGAAAACCTGATC+AGG - chr4.4:32723798-32723817 None:intergenic 35.0%
TCCAAACAAATGCCCAAAAA+AGG - chr4.4:32724713-32724732 None:intergenic 35.0%
TCGCTATAGCATTATTGAGT+TGG - chr4.4:32723360-32723379 None:intergenic 35.0%
TGACGACAATTATGTTCTCA+AGG + chr4.4:32725339-32725358 MS.gene73872:CDS 35.0%
TGTGAGAAGGTAGAAAGTAA+AGG + chr4.4:32723239-32723258 MS.gene73872:CDS 35.0%
TTAAATAGACACAGCGTCTT+GGG + chr4.4:32724146-32724165 MS.gene73872:intron 35.0%
TTAAATCAATTAGAGTGGGC+TGG - chr4.4:32724232-32724251 None:intergenic 35.0%
TTAGATCAGAACATCACTGA+AGG - chr4.4:32724107-32724126 None:intergenic 35.0%
TTCTTCAATATCACTCAGAC+TGG - chr4.4:32724029-32724048 None:intergenic 35.0%
! AAAAGCACAGTTCTCAATCA+TGG + chr4.4:32723317-32723336 MS.gene73872:intron 35.0%
! AACATGTTTGAAACTGACTG+AGG - chr4.4:32725472-32725491 None:intergenic 35.0%
! ACTCTGGTTGCCTATTTTAT+GGG + chr4.4:32724802-32724821 MS.gene73872:intron 35.0%
! ATACACTAGTTTCCACTTTC+CGG - chr4.4:32725163-32725182 None:intergenic 35.0%
! ATTTTCTGATCAAGCAATGG+TGG + chr4.4:32723765-32723784 MS.gene73872:intron 35.0%
! GCAGATAAATTTTCCAACAC+AGG - chr4.4:32725273-32725292 None:intergenic 35.0%
! TACTCTGGTTGCCTATTTTA+TGG + chr4.4:32724801-32724820 MS.gene73872:intron 35.0%
! TCTGAGGCTAATAATTACCA+CGG - chr4.4:32723574-32723593 None:intergenic 35.0%
! TGATGCTCTGAATGTTCTTA+TGG + chr4.4:32723946-32723965 MS.gene73872:CDS 35.0%
! TTGTTTAGTTCAGCTTACTC+TGG + chr4.4:32724786-32724805 MS.gene73872:intron 35.0%
! TTTTGTGGTATCATAAAGCC+CGG + chr4.4:32725141-32725160 MS.gene73872:intron 35.0%
!!! ATTAAGCACGGTGGTTTTTT+GGG + chr4.4:32723538-32723557 MS.gene73872:intron 35.0%
AAAGGCAGATACTGAGATGA+TGG - chr4.4:32724486-32724505 None:intergenic 40.0%
AATTGTGAAACTTGCAGCAG+TGG + chr4.4:32723107-32723126 MS.gene73872:intron 40.0%
ACTCAGCATTCAGCATACTA+AGG - chr4.4:32723076-32723095 None:intergenic 40.0%
AGGATAAGTGCAACTTTGCA+AGG - chr4.4:32722814-32722833 None:intergenic 40.0%
AGTTAATTCCACTAACCAGC+TGG - chr4.4:32724677-32724696 None:intergenic 40.0%
ATAAGTCTTGGGAGCTAGTT+AGG + chr4.4:32725228-32725247 MS.gene73872:intron 40.0%
ATGAGAAAAACATCCGTCGA+AGG + chr4.4:32723918-32723937 MS.gene73872:CDS 40.0%
CTCAGCATTCAGCATACTAA+GGG - chr4.4:32723075-32723094 None:intergenic 40.0%
CTCCGGCAATTTAGTATGAA+AGG + chr4.4:32722727-32722746 MS.gene73872:CDS 40.0%
GGCTTAGGAGTAGAATTGAA+AGG + chr4.4:32724167-32724186 MS.gene73872:CDS 40.0%
GTTACACACAATATTGCGGA+TGG + chr4.4:32725403-32725422 MS.gene73872:CDS 40.0%
TAAGTCTTGGGAGCTAGTTA+GGG + chr4.4:32725229-32725248 MS.gene73872:intron 40.0%
TACCAAGAAGAAAAAGCGAC+GGG + chr4.4:32722676-32722695 MS.gene73872:CDS 40.0%
TCAGCATTCAGCATACTAAG+GGG - chr4.4:32723074-32723093 None:intergenic 40.0%
TCCACTCCCTGGAATATTAA+AGG + chr4.4:32725513-32725532 MS.gene73872:CDS 40.0%
TCCTATTGTGGTCAAACACT+CGG + chr4.4:32725182-32725201 MS.gene73872:intron 40.0%
TGAGAAAAACATCCGTCGAA+GGG + chr4.4:32723919-32723938 MS.gene73872:CDS 40.0%
TGCTTCGATAAGCTATTGTG+AGG - chr4.4:32722928-32722947 None:intergenic 40.0%
TGTGTAACATTGTCACTCTG+TGG - chr4.4:32725391-32725410 None:intergenic 40.0%
TTAATACCGGAATGCTAACC+TGG + chr4.4:32724248-32724267 MS.gene73872:intron 40.0%
TTGTCAATGATTAAGCACGG+TGG + chr4.4:32723529-32723548 MS.gene73872:intron 40.0%
TTTAATATTCCAGGGAGTGG+AGG - chr4.4:32725514-32725533 None:intergenic 40.0%
! ATTTTCTGCAGTGTGTGAGA+AGG + chr4.4:32723226-32723245 MS.gene73872:intron 40.0%
! CATCTCAGTATCTGCCTTTT+TGG + chr4.4:32724487-32724506 MS.gene73872:intron 40.0%
! TACACTAGTTTCCACTTTCC+GGG - chr4.4:32725162-32725181 None:intergenic 40.0%
! TGAGAACTGTGCTTTTAACG+TGG - chr4.4:32723313-32723332 None:intergenic 40.0%
!! ACTTTCTAAGATCCATGACC+AGG - chr4.4:32724856-32724875 None:intergenic 40.0%
!! GTTCTCAAGGCAATGGATTT+TGG + chr4.4:32725352-32725371 MS.gene73872:CDS 40.0%
!! TCAAGGCAATGGATTTTGGT+GGG + chr4.4:32725356-32725375 MS.gene73872:CDS 40.0%
!!! CCCTTTTTTGGGCATTTGTT+TGG + chr4.4:32724709-32724728 MS.gene73872:intron 40.0%
!!! GATTAAGCACGGTGGTTTTT+TGG + chr4.4:32723537-32723556 MS.gene73872:intron 40.0%
AAGTCCACTACCAGTTTCTG+AGG - chr4.4:32723590-32723609 None:intergenic 45.0%
ACAACATGAAATGGGTAGGC+AGG - chr4.4:32723621-32723640 None:intergenic 45.0%
ACACAATATTGCGGATGGAG+GGG + chr4.4:32725408-32725427 MS.gene73872:CDS 45.0%
ACACACAATATTGCGGATGG+AGG + chr4.4:32725406-32725425 MS.gene73872:CDS 45.0%
AGCAATCCAGGTTAGCATTC+CGG - chr4.4:32724257-32724276 None:intergenic 45.0%
AGGTAAAGATACACCTCCAC+TGG - chr4.4:32724443-32724462 None:intergenic 45.0%
ATAAGAATGGAAGAGGGCTC+CGG + chr4.4:32722710-32722729 MS.gene73872:CDS 45.0%
ATACGGAGAATTCCTGGTCA+TGG + chr4.4:32724841-32724860 MS.gene73872:intron 45.0%
ATAGGTGGCAGATGAACTTG+TGG + chr4.4:32723738-32723757 MS.gene73872:intron 45.0%
ATATCACTCAGACTGGTACG+AGG - chr4.4:32724022-32724041 None:intergenic 45.0%
CACACAATATTGCGGATGGA+GGG + chr4.4:32725407-32725426 MS.gene73872:CDS 45.0%
GATCTTATCCAGCTGGTTAG+TGG + chr4.4:32724666-32724685 MS.gene73872:intron 45.0%
GCCTTTAATATTCCAGGGAG+TGG - chr4.4:32725517-32725536 None:intergenic 45.0%
GGGGTGATAAGAATGGAAGA+GGG + chr4.4:32722704-32722723 MS.gene73872:CDS 45.0%
GGGTTTATACGGAGAATTCC+TGG + chr4.4:32724835-32724854 MS.gene73872:intron 45.0%
GGTAAAGATACACCTCCACT+GGG - chr4.4:32724442-32724461 None:intergenic 45.0%
GTATCATAAAGCCCGGAAAG+TGG + chr4.4:32725148-32725167 MS.gene73872:intron 45.0%
GTCAGGCATGTATCAATCAC+AGG + chr4.4:32725444-32725463 MS.gene73872:CDS 45.0%
TCCGAGTGTTTGACCACAAT+AGG - chr4.4:32725186-32725205 None:intergenic 45.0%
TGGGGTGATAAGAATGGAAG+AGG + chr4.4:32722703-32722722 MS.gene73872:CDS 45.0%
TTAGCCTCAGAAACTGGTAG+TGG + chr4.4:32723583-32723602 MS.gene73872:intron 45.0%
! CTGTATCTTTTCTCTCTGCG+TGG + chr4.4:32724070-32724089 MS.gene73872:intron 45.0%
!! AAGGCAATGGATTTTGGTGG+GGG + chr4.4:32725358-32725377 MS.gene73872:CDS 45.0%
!! CAAGGCAATGGATTTTGGTG+GGG + chr4.4:32725357-32725376 MS.gene73872:CDS 45.0%
!! CTCAAGGCAATGGATTTTGG+TGG + chr4.4:32725355-32725374 MS.gene73872:CDS 45.0%
!!! TTTTGGGATCAAGTTGTCCG+TGG + chr4.4:32723554-32723573 MS.gene73872:intron 45.0%
!! AAAAAAAAATATAAGTAATA+CGG - chr4.4:32723658-32723677 None:intergenic 5.0%
!!! TTTAATCAAATTTATATTTT+TGG - chr4.4:32724131-32724150 None:intergenic 5.0%
AAAGATACACCTCCACTGGG+AGG - chr4.4:32724439-32724458 None:intergenic 50.0%
AAGGAAAGCAGCCTATCTGC+AGG + chr4.4:32724186-32724205 MS.gene73872:CDS 50.0%
AATATTGCGGATGGAGGGGA+AGG + chr4.4:32725412-32725431 MS.gene73872:CDS 50.0%
GAGCATCATAGACCCTTCGA+CGG - chr4.4:32723934-32723953 None:intergenic 50.0%
GGCTGTTGGGGTGATAAGAA+TGG + chr4.4:32722697-32722716 MS.gene73872:CDS 50.0%
TCCACTTCCACAGTTGCATG+AGG - chr4.4:32724988-32725007 None:intergenic 50.0%
TCCTCATGCAACTGTGGAAG+TGG + chr4.4:32724984-32725003 MS.gene73872:CDS 50.0%
TGCTGTGATGTGGCTCCTAT+TGG + chr4.4:32724916-32724935 MS.gene73872:intron 50.0%
TTGTCGTCATGCAGCTCGAA+AGG - chr4.4:32725328-32725347 None:intergenic 50.0%
! ATCTGTTGGCTGCTGTGATG+TGG + chr4.4:32724906-32724925 MS.gene73872:intron 50.0%
! TGGTGCTTTTGTCCCTGTGT+TGG + chr4.4:32725257-32725276 MS.gene73872:intron 50.0%
!! AAGAAAAAGCGACGGGCTGT+TGG + chr4.4:32722683-32722702 MS.gene73872:CDS 50.0%
!! AGAAAAAGCGACGGGCTGTT+GGG + chr4.4:32722684-32722703 MS.gene73872:CDS 50.0%
!!! AGCCCGTCGCTTTTTCTTCT+TGG - chr4.4:32722681-32722700 None:intergenic 50.0%
AGACACAGCGTCTTGGGCTT+AGG + chr4.4:32724152-32724171 MS.gene73872:intron 55.0%
AGCTCAGGAAATCCTCCCAG+TGG + chr4.4:32724424-32724443 MS.gene73872:CDS 55.0%
CATGAAATGGGTAGGCAGGC+AGG - chr4.4:32723617-32723636 None:intergenic 55.0%
CTGCTCAAGCTCCTGCAGAT+AGG - chr4.4:32724200-32724219 None:intergenic 55.0%
GACACGTCCTCATGCAACTG+TGG + chr4.4:32724978-32724997 MS.gene73872:intron 55.0%
GTCTTGGGAGCTAGTTAGGG+TGG + chr4.4:32725232-32725251 MS.gene73872:intron 55.0%
TCAGGAAATCCTCCCAGTGG+AGG + chr4.4:32724427-32724446 MS.gene73872:CDS 55.0%
! GGGGAAGGTTCTAGCATGTC+AGG + chr4.4:32725427-32725446 MS.gene73872:CDS 55.0%
! TGGTGGTTTGTCCCCTGATC+AGG + chr4.4:32723782-32723801 MS.gene73872:intron 55.0%
!! GAAAAAGCGACGGGCTGTTG+GGG + chr4.4:32722685-32722704 MS.gene73872:CDS 55.0%
CCAGGGAGTGGAGGTGATGT+CGG - chr4.4:32725505-32725524 None:intergenic 60.0%
GGGAGCTAGTTAGGGTGGTG+TGG + chr4.4:32725237-32725256 MS.gene73872:intron 60.0%
CCGACATCACCTCCACTCCC+TGG + chr4.4:32725502-32725521 MS.gene73872:CDS 65.0%
GGGAGTGGAGGTGATGTCGG+AGG - chr4.4:32725502-32725521 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 32722676 32725558 32722676 ID=MS.gene73872
chr4.4 mRNA 32722676 32725558 32722676 ID=MS.gene73872.t1;Parent=MS.gene73872
chr4.4 exon 32722676 32722748 32722676 ID=MS.gene73872.t1.exon1;Parent=MS.gene73872.t1
chr4.4 CDS 32722676 32722748 32722676 ID=cds.MS.gene73872.t1;Parent=MS.gene73872.t1
chr4.4 exon 32723237 32723301 32723237 ID=MS.gene73872.t1.exon2;Parent=MS.gene73872.t1
chr4.4 CDS 32723237 32723301 32723237 ID=cds.MS.gene73872.t1;Parent=MS.gene73872.t1
chr4.4 exon 32723742 32723783 32723742 ID=MS.gene73872.t1.exon3;Parent=MS.gene73872.t1
chr4.4 CDS 32723742 32723783 32723742 ID=cds.MS.gene73872.t1;Parent=MS.gene73872.t1
chr4.4 exon 32723908 32724054 32723908 ID=MS.gene73872.t1.exon4;Parent=MS.gene73872.t1
chr4.4 CDS 32723908 32724054 32723908 ID=cds.MS.gene73872.t1;Parent=MS.gene73872.t1
chr4.4 exon 32724154 32724222 32724154 ID=MS.gene73872.t1.exon5;Parent=MS.gene73872.t1
chr4.4 CDS 32724154 32724222 32724154 ID=cds.MS.gene73872.t1;Parent=MS.gene73872.t1
chr4.4 exon 32724379 32724477 32724379 ID=MS.gene73872.t1.exon6;Parent=MS.gene73872.t1
chr4.4 CDS 32724379 32724477 32724379 ID=cds.MS.gene73872.t1;Parent=MS.gene73872.t1
chr4.4 exon 32724979 32725049 32724979 ID=MS.gene73872.t1.exon7;Parent=MS.gene73872.t1
chr4.4 CDS 32724979 32725049 32724979 ID=cds.MS.gene73872.t1;Parent=MS.gene73872.t1
chr4.4 exon 32725321 32725558 32725321 ID=MS.gene73872.t1.exon8;Parent=MS.gene73872.t1
chr4.4 CDS 32725321 32725558 32725321 ID=cds.MS.gene73872.t1;Parent=MS.gene73872.t1
Gene Sequence

>MS.gene73872

TACCAAGAAGAAAAAGCGACGGGCTGTTGGGGTGATAAGAATGGAAGAGGGCTCCGGCAATTTAGTATGAAAGGTTTTTCATCTGTCTTGTTTGACCACTTTGATCTTTAACTTTTTACTAGTAATTTACTTTATCCTTGCAAAGTTGCACTTATCCTTGTTTGAAACAACTTATTTGAACTTATGACATAAACATGTGTGTAAAGGTTTGGAAAGTTTATGCAAATAGCTTATTATATGTCACAAGCTCCTCACAATAGCTTATCGAAGCAATTTATACAGAGCTTATGCAGCGTTTGTATTAATTTTTGACTGTTGAAAGAGTTTATGCATAAGCTCTTGGATGATAAAACACTTATTGAAATAGCACTTAATTAACTTATTTACTGAAATGACCCCTTAGTATGCTGAATGCTGAGTATTTAGACGATAATTGTGAAACTTGCAGCAGTGGTTCTTTTGTTTCACAGACAGAGATTTATTTTATTCAGGCCATTTTTGACAATTTTCTCTACTTTTTGGTGGTTTTCCCCTCTCTAATTTACTGTTGATTTTCTGCAGTGTGTGAGAAGGTAGAAAGTAAAGGAAGAACAACTTACATGAGGTTAGGCCTTTACTTTAAACCAGTTTTTTACCACGTTAAAAGCACAGTTCTCAATCATGGATAGCAAATCGGAGCAACCAACTCAATAATGCTATAGCGAGATTACCACGTTCTAAATATAAAGTCTTTTATACAGATTTAATAAATGATTCTATAAATACATACATGTTGGAAATGTAAACTAATTACTAAAAGTTCATGTATATCATAGAATAGAATCGTTACCTTCAATTAGTTTTTTAATAGGTTTTGTCAATGATTAAGCACGGTGGTTTTTTGGGATCAAGTTGTCCGTGGTAATTATTAGCCTCAGAAACTGGTAGTGGACTTGAAGCCTGCCTGCCTACCCATTTCATGTTGTGATTTATCATTCATCCGTATTACTTATATTTTTTTTTCTTCTAGTTATTAATCCTTATTTCCTAAAAAAAAAATTTTTTTTTTTTTTTTTTTTAAATATAGGTGGCAGATGAACTTGTGGCTGAATTTTCTGATCAAGCAATGGTGGTTTGTCCCCTGATCAGGTTTTCTTATCTTCACTAACAATTTCAATATTATTTGCTATTACACATTAAGGTGTAAGATAAATCTTATGAACTTATTTTTTTGGGAAAATGAAACTTTTCAGCAACAATATGATGAGAAAAACATCCGTCGAAGGGTCTATGATGCTCTGAATGTTCTTATGGCTATGAATATTATTTCCAAGGATAAAAAAGAAATACAATGGAAGGGTCTTCCTCGTACCAGTCTGAGTGATATTGAAGAACTAAAGGTTTGTCTTCTTTAGCTGTATCTTTTCTCTCTGCGTGGCTTATTCTTTACCTTCAGTGATGTTCTGATCTAATCCAAAAATATAAATTTGATTAAATAGACACAGCGTCTTGGGCTTAGGAGTAGAATTGAAAGGAAAGCAGCCTATCTGCAGGAGCTTGAGCAGCAAGTAAGTCCAGCCCACTCTAATTGATTTAATACCGGAATGCTAACCTGGATTGCTATTTTTTAAATTTGCACTTGATTATAAGAAGATTCTATCAAACTACGCTGTTTTTGTGTTGTTCAGTTTCTGTTTGCTAACTGCTTTTATTATTGCTACAAGTTTGTAGGTCTTCAGAACCTTATTCAACGAAATGAACAATTATATAGCTCAGGAAATCCTCCCAGTGGAGGTGTATCTTTACCTTTTATTCTGGTTCAGGTATACCATCATCTCAGTATCTGCCTTTTTGGTCATTTTGTCCAGTAGAAATTGTTGTTCAATTATGCCTTGGATGATAATATATTACATTGATATGAACTCACTACTGAGCATATTACATGAACTTAAGTAGTTGGCTTGGATTTTGACTTATGAACATTTTAATTATAAAGTATTTTATGAAAATAGATCTTATCCAGCTGGTTAGTGGAATTAACTAGAAATAATTTTCCCTTTTTTGGGCATTTGTTTGGATTTTATCGACCGAAAGTTACTGCTATATACTAGATGGATTTTATCAGCTCATATTGTTTAGTTCAGCTTACTCTGGTTGCCTATTTTATGGGTCTATGTTATGGGTTTATACGGAGAATTCCTGGTCATGGATCTTAGAAAGTTGCTAATTTATTTTAATGAATAATATGCTCATCTGTTGGCTGCTGTGATGTGGCTCCTATTGGTGCTGATATTTGATTAATCTCATGCTTCTAAAATCTGTAGACACGTCCTCATGCAACTGTGGAAGTGGAAATATCAGAAGACATGCAGCTTGTTCATTTTGATTTCAATAGGTGAAGCTTCACTTGCCTTGCTTGTCATTATCTGAGTTATGATGTGATTAGCTTTAGGTTTGTACGCCGTCTTAATGTTTAGACTTCCTAATTTTGTGGTATCATAAAGCCCGGAAAGTGGAAACTAGTGTATCCTATTGTGGTCAAACACTCGGACTACTAAAGAATTTTCAAGTAGATAAGTCTTGGGAGCTAGTTAGGGTGGTGTGGTGCTTTTGTCCCTGTGTTGGAAAATTTATCTGCCACTTATTTATGTTTACTCTCTCTGCAGTTCTCCTTTCGAGCTGCATGACGACAATTATGTTCTCAAGGCAATGGATTTTGGTGGGGGTGAGAGACCACAGAGTGACAATGTTACACACAATATTGCGGATGGAGGGGAAGGTTCTAGCATGTCAGGCATGTATCAATCACAGGTTCCTCAGTCAGTTTCAAACATGTTAGTTAGGCCTCCGACATCACCTCCACTCCCTGGAATATTAAAGGCAAGAGTTAAGCAAGAGAATTGA
Protein sequence

>MS.gene73872.t1

YQEEKATGCWGDKNGRGLRQFSMKVCEKVESKGRTTYMRLGLYFKPVADELVAEFSDQAMQQYDEKNIRRRVYDALNVLMAMNIISKDKKEIQWKGLPRTSLSDIEELKTQRLGLRSRIERKAAYLQELEQQFVGLQNLIQRNEQLYSSGNPPSGGVSLPFILVQTRPHATVEVEISEDMQLVHFDFNSSPFELHDDNYVLKAMDFGGGERPQSDNVTHNIADGGEGSSMSGMYQSQVPQSVSNMLVRPPTSPPLPGILKARVKQEN