Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74828.t1 | AEX20345.1 | 67.6 | 401 | 63 | 3 | 1 | 341 | 66 | 459 | 1.40E-125 | 459.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74828.t1 | H2EIY5 | 67.6 | 401 | 63 | 3 | 1 | 341 | 66 | 459 | 9.7e-126 | 459.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05415 | MS.gene74828 | 0.814466 | 1.52E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74828.t1 | MTR_3g114180 | 83.969 | 131 | 19 | 1 | 216 | 346 | 7 | 135 | 1.47e-70 | 217 |
| MS.gene74828.t1 | MTR_3g114190 | 82.734 | 139 | 21 | 2 | 206 | 344 | 228 | 363 | 4.46e-69 | 221 |
| MS.gene74828.t1 | MTR_3g114190 | 85.271 | 129 | 17 | 1 | 216 | 344 | 7 | 133 | 9.21e-68 | 218 |
| MS.gene74828.t1 | MTR_3g113780 | 78.182 | 110 | 24 | 0 | 221 | 330 | 1 | 110 | 1.43e-55 | 177 |
| MS.gene74828.t1 | MTR_3g113780 | 76.744 | 86 | 20 | 0 | 221 | 306 | 1 | 86 | 4.74e-41 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAAATTTAATTAGAGA+CGG | + | 23879:95293-95312 | None:intergenic | 15.0% |
| !! | AAACACTACAAAAAAAAATG+TGG | + | 23879:95243-95262 | None:intergenic | 20.0% |
| !! | AACACTACAAAAAAAAATGT+GGG | + | 23879:95242-95261 | None:intergenic | 20.0% |
| !! | ATGAAATAATAAGAAACCAA+AGG | + | 23879:95909-95928 | None:intergenic | 20.0% |
| !! | TGAAATAATAAGAAACCAAA+GGG | + | 23879:95908-95927 | None:intergenic | 20.0% |
| !! | TTGGAATTTAATATTGTATC+TGG | - | 23879:95755-95774 | MS.gene74828:CDS | 20.0% |
| ! | AAAATTTACCAAGTATGAAG+CGG | + | 23879:95170-95189 | None:intergenic | 25.0% |
| ! | AAGAATACTTGAATGAGAAT+AGG | + | 23879:95847-95866 | None:intergenic | 25.0% |
| ! | TTTAAACTCAATTGAATAGC+AGG | + | 23879:95216-95235 | None:intergenic | 25.0% |
| ! | TTTAATTAGAGACGGAAAAA+AGG | + | 23879:95285-95304 | None:intergenic | 25.0% |
| !!! | AGATCTTAAGCATTTTTTAG+AGG | - | 23879:95325-95344 | MS.gene74828:intron | 25.0% |
| !!! | TTTTTGTAGTGTTTAGTTTG+AGG | - | 23879:95249-95268 | MS.gene74828:CDS | 25.0% |
| AAATTTACCAAGTATGAAGC+GGG | + | 23879:95169-95188 | None:intergenic | 30.0% | |
| ACTTGAATGAGAATAGGATT+AGG | + | 23879:95841-95860 | None:intergenic | 30.0% | |
| TAAGCAATATCCTAAAACTG+AGG | - | 23879:95421-95440 | MS.gene74828:CDS | 30.0% | |
| TTCTTTGACTGTTGTAATTC+TGG | - | 23879:95377-95396 | MS.gene74828:intron | 30.0% | |
| ! | AAAATGTAGAAAGCACAAAG+GGG | - | 23879:95976-95995 | MS.gene74828:CDS | 30.0% |
| ! | CAAAATGTAGAAAGCACAAA+GGG | - | 23879:95975-95994 | MS.gene74828:CDS | 30.0% |
| ! | GATCAAATTGATTTACAACC+TGG | - | 23879:95710-95729 | MS.gene74828:CDS | 30.0% |
| ! | GTTTCTTCTCTTGTTTCATA+TGG | - | 23879:96192-96211 | MS.gene74828:intron | 30.0% |
| !! | TAAGATCAAACCTTTGAAAC+TGG | - | 23879:95619-95638 | MS.gene74828:intron | 30.0% |
| !!! | AAAGTTATCGGTTTTTACCT+GGG | - | 23879:95565-95584 | MS.gene74828:CDS | 30.0% |
| !!! | TCTCTCTTTTTTTACATGTC+TGG | - | 23879:95075-95094 | MS.gene74828:CDS | 30.0% |
| !!! | TTTTTTTACATGTCTGGTCA+TGG | - | 23879:95081-95100 | MS.gene74828:CDS | 30.0% |
| AACTTCTTCATGAGAAGAAG+CGG | - | 23879:95946-95965 | MS.gene74828:CDS | 35.0% | |
| AATGGATCAGCTCTTTCAAA+TGG | - | 23879:95029-95048 | MS.gene74828:CDS | 35.0% | |
| AATTTACCAAGTATGAAGCG+GGG | + | 23879:95168-95187 | None:intergenic | 35.0% | |
| ACTGACAATCACAACACAAA+AGG | + | 23879:95736-95755 | None:intergenic | 35.0% | |
| AGACTTGGGATCAAAGTTAT+CGG | - | 23879:95553-95572 | MS.gene74828:CDS | 35.0% | |
| CAAATAATAACCTCACCCTT+TGG | - | 23879:95890-95909 | MS.gene74828:CDS | 35.0% | |
| GAAGAAACAAACATAAGCTG+AGG | + | 23879:96180-96199 | None:intergenic | 35.0% | |
| GATTTATAGACACTCCTTCT+TGG | + | 23879:95355-95374 | None:intergenic | 35.0% | |
| GCCAAAAGAATTTGAATACC+CGG | - | 23879:96076-96095 | MS.gene74828:CDS | 35.0% | |
| GCTGTTAGAAAGACATTGAT+CGG | + | 23879:96354-96373 | None:intergenic | 35.0% | |
| TAATAAGAAACCAAAGGGTG+AGG | + | 23879:95903-95922 | None:intergenic | 35.0% | |
| TAGATACAATAGACCTCAAG+TGG | - | 23879:96248-96267 | MS.gene74828:intron | 35.0% | |
| TTCTTGGACAAGAAGAGAAA+CGG | - | 23879:95105-95124 | MS.gene74828:CDS | 35.0% | |
| TTTCTAACAGCAAGCATACT+TGG | - | 23879:96363-96382 | MS.gene74828:CDS | 35.0% | |
| TTTGATCTTAGTCCCAAACT+CGG | + | 23879:95609-95628 | None:intergenic | 35.0% | |
| TTTGTGTTGTGATTGTCAGT+TGG | - | 23879:95736-95755 | MS.gene74828:CDS | 35.0% | |
| ! | AAATGTAGAAAGCACAAAGG+GGG | - | 23879:95977-95996 | MS.gene74828:CDS | 35.0% |
| ! | ACCGGGTATTCAAATTCTTT+TGG | + | 23879:96080-96099 | None:intergenic | 35.0% |
| ! | GCAAAATGTAGAAAGCACAA+AGG | - | 23879:95974-95993 | MS.gene74828:CDS | 35.0% |
| !! | AAAAAGAGCGTCTTGAGAAA+GGG | - | 23879:96461-96480 | MS.gene74828:CDS | 35.0% |
| !! | GCTATTTTCATGCAAGACTT+GGG | - | 23879:95539-95558 | MS.gene74828:CDS | 35.0% |
| !! | TTTTTAGAGGAAGTCCAAGA+AGG | - | 23879:95338-95357 | MS.gene74828:intron | 35.0% |
| !!! | AAAGAGAGAACCTCCTTTTT+TGG | + | 23879:95064-95083 | None:intergenic | 35.0% |
| !!! | CAAAGTTATCGGTTTTTACC+TGG | - | 23879:95564-95583 | MS.gene74828:CDS | 35.0% |
| !!! | TGCTATTTTCATGCAAGACT+TGG | - | 23879:95538-95557 | MS.gene74828:CDS | 35.0% |
| AGGTTGAGTTTACCCATTTG+CGG | + | 23879:95821-95840 | None:intergenic | 40.0% | |
| ATCAGCTCTTTCAAATGGCT+AGG | - | 23879:95034-95053 | MS.gene74828:CDS | 40.0% | |
| ATCTGTTCCACCAGTTTCAA+AGG | + | 23879:95632-95651 | None:intergenic | 40.0% | |
| CATACTTGGAGCATGAGAAA+AGG | - | 23879:96377-96396 | MS.gene74828:CDS | 40.0% | |
| CTTTCAACTTTCTGCTCCTT+TGG | + | 23879:96023-96042 | None:intergenic | 40.0% | |
| GAGGGAGAGTTTCTTTATGA+TGG | - | 23879:96047-96066 | MS.gene74828:CDS | 40.0% | |
| GTTTACGAAGAGACGTTGAA+AGG | - | 23879:96098-96117 | MS.gene74828:CDS | 40.0% | |
| TTCAACGTCTCTTCGTAAAC+CGG | + | 23879:96098-96117 | None:intergenic | 40.0% | |
| ! | ACTCTTCAAAAAGCATGCAG+AGG | - | 23879:96408-96427 | MS.gene74828:CDS | 40.0% |
| ! | ATCAGCATCCTTGTTGGTTA+TGG | - | 23879:95129-95148 | MS.gene74828:CDS | 40.0% |
| ! | GAAGCACAAGTTGACTTTGA+AGG | + | 23879:95455-95474 | None:intergenic | 40.0% |
| !! | CAGAAAGTTGAAAGCACTGA+GGG | - | 23879:96029-96048 | MS.gene74828:CDS | 40.0% |
| !! | GAAAAAGAGCGTCTTGAGAA+AGG | - | 23879:96460-96479 | MS.gene74828:CDS | 40.0% |
| !! | GATCAAACCTTTGAAACTGG+TGG | - | 23879:95622-95641 | MS.gene74828:intron | 40.0% |
| !! | GATTCTGCTTCGTTTTAGCA+TGG | + | 23879:95661-95680 | None:intergenic | 40.0% |
| AACGGAATCAGCATCCTTGT+TGG | - | 23879:95123-95142 | MS.gene74828:CDS | 45.0% | |
| AGAGAAAAACTCGCCTCATC+AGG | + | 23879:96286-96305 | None:intergenic | 45.0% | |
| AGCATGCAGAGGAAAAGAAG+CGG | - | 23879:96419-96438 | MS.gene74828:CDS | 45.0% | |
| ATCGCTACGGAATTGAAGGT+GGG | - | 23879:95683-95702 | MS.gene74828:intron | 45.0% | |
| ATGGCTAGGAAGGCCAAAAA+AGG | - | 23879:95048-95067 | MS.gene74828:CDS | 45.0% | |
| CAATCACAACACAAAAGGCC+AGG | + | 23879:95731-95750 | None:intergenic | 45.0% | |
| CATGTCTGGTCATGGATTCT+TGG | - | 23879:95089-95108 | MS.gene74828:CDS | 45.0% | |
| GAAGCAGAATCAGATCGCTA+CGG | - | 23879:95670-95689 | MS.gene74828:intron | 45.0% | |
| GCTCTTTCAAATGGCTAGGA+AGG | - | 23879:95038-95057 | MS.gene74828:CDS | 45.0% | |
| TACGGAATTGAAGGTGGGAA+TGG | - | 23879:95688-95707 | MS.gene74828:intron | 45.0% | |
| TATCCATGGGAAGAAGCCAA+AGG | - | 23879:96004-96023 | MS.gene74828:CDS | 45.0% | |
| TCAACGTCTCTTCGTAAACC+GGG | + | 23879:96097-96116 | None:intergenic | 45.0% | |
| TCAACTTGTGCTTCCCACAA+AGG | - | 23879:95461-95480 | MS.gene74828:CDS | 45.0% | |
| TCAGATCGCTACGGAATTGA+AGG | - | 23879:95679-95698 | MS.gene74828:intron | 45.0% | |
| TCCTGTACGTGAATGACATG+CGG | + | 23879:95510-95529 | None:intergenic | 45.0% | |
| TCTTCATGAGAAGAAGCGGA+AGG | - | 23879:95950-95969 | MS.gene74828:CDS | 45.0% | |
| TGCAAGTTCTCACCGAGTTT+GGG | - | 23879:95594-95613 | MS.gene74828:intron | 45.0% | |
| TTGAGAAAGGGAAGGGAAAG+AGG | - | 23879:96473-96492 | MS.gene74828:CDS | 45.0% | |
| TTGAGTTTACCCATTTGCGG+CGG | + | 23879:95818-95837 | None:intergenic | 45.0% | |
| ! | CATCCTTGTTGGTTATGGTG+AGG | - | 23879:95134-95153 | MS.gene74828:CDS | 45.0% |
| ! | CTTGTTGGTTATGGTGAGGA+CGG | - | 23879:95138-95157 | MS.gene74828:CDS | 45.0% |
| ! | GCACAAGTTGACTTTGAAGG+AGG | + | 23879:95452-95471 | None:intergenic | 45.0% |
| ! | GGAGGACTATCCTCAGTTTT+AGG | + | 23879:95434-95453 | None:intergenic | 45.0% |
| ! | TTGTTGGTTATGGTGAGGAC+GGG | - | 23879:95139-95158 | MS.gene74828:CDS | 45.0% |
| ! | TTTGGCTTCTTCCCATGGAT+AGG | + | 23879:96005-96024 | None:intergenic | 45.0% |
| !! | GCAGAAAGTTGAAAGCACTG+AGG | - | 23879:96028-96047 | MS.gene74828:CDS | 45.0% |
| ACAAAGGGGGATCCTATCCA+TGG | - | 23879:95990-96009 | MS.gene74828:CDS | 50.0% | |
| ACCGCATGTCATTCACGTAC+AGG | - | 23879:95506-95525 | MS.gene74828:CDS | 50.0% | |
| AGCAGACAGAGAGAGAGAGA+GGG | + | 23879:96137-96156 | None:intergenic | 50.0% | |
| ATGCAGAGGAAAAGAAGCGG+TGG | - | 23879:96422-96441 | MS.gene74828:CDS | 50.0% | |
| CAAAGGGGGATCCTATCCAT+GGG | - | 23879:95991-96010 | MS.gene74828:CDS | 50.0% | |
| CGTCCTCACCATAACCAACA+AGG | + | 23879:95140-95159 | None:intergenic | 50.0% | |
| GAGCGTCTTGAGAAAGGGAA+GGG | - | 23879:96466-96485 | MS.gene74828:CDS | 50.0% | |
| GATCGCTACGGAATTGAAGG+TGG | - | 23879:95682-95701 | MS.gene74828:intron | 50.0% | |
| GCTAGGAAGGCCAAAAAAGG+AGG | - | 23879:95051-95070 | MS.gene74828:CDS | 50.0% | |
| GTAGAACTGGACACCACTTG+AGG | + | 23879:96264-96283 | None:intergenic | 50.0% | |
| GTGCAAGTTCTCACCGAGTT+TGG | - | 23879:95593-95612 | MS.gene74828:intron | 50.0% | |
| GTGTCCAGTTCTACCTGATG+AGG | - | 23879:96270-96289 | MS.gene74828:intron | 50.0% | |
| TGTATCTGGACTCTCCCCAA+TGG | - | 23879:95769-95788 | MS.gene74828:CDS | 50.0% | |
| ! | AGAGCGTCTTGAGAAAGGGA+AGG | - | 23879:96465-96484 | MS.gene74828:CDS | 50.0% |
| ! | CTCTGTCTGCTGCTTGCATT+TGG | - | 23879:96146-96165 | MS.gene74828:CDS | 50.0% |
| ! | TGTTGGTTATGGTGAGGACG+GGG | - | 23879:95140-95159 | MS.gene74828:intron | 50.0% |
| AGACAGAGAGAGAGAGAGGG+AGG | + | 23879:96134-96153 | None:intergenic | 55.0% | |
| CAGCAGACAGAGAGAGAGAG+AGG | + | 23879:96138-96157 | None:intergenic | 55.0% | |
| CGGTAAATACCGTCCCCAAG+GGG | + | 23879:95490-95509 | None:intergenic | 55.0% | |
| CTCGCCTCATCAGGTAGAAC+TGG | + | 23879:96277-96296 | None:intergenic | 55.0% | |
| GACAGAGAGAGAGAGAGGGA+GGG | + | 23879:96133-96152 | None:intergenic | 55.0% | |
| GAGAACTTGCACAAGTGCCC+AGG | + | 23879:95585-95604 | None:intergenic | 55.0% | |
| GCGGTAAATACCGTCCCCAA+GGG | + | 23879:95491-95510 | None:intergenic | 55.0% | |
| TCCCACAAAGGTCACCCCTT+GGG | - | 23879:95473-95492 | MS.gene74828:CDS | 55.0% | |
| TGCGGTAAATACCGTCCCCA+AGG | + | 23879:95492-95511 | None:intergenic | 55.0% | |
| TTCCCACAAAGGTCACCCCT+TGG | - | 23879:95472-95491 | MS.gene74828:CDS | 55.0% | |
| ! | GCTCCTTTGGCTTCTTCCCA+TGG | + | 23879:96010-96029 | None:intergenic | 55.0% |
| CAAAGGTCACCCCTTGGGGA+CGG | - | 23879:95478-95497 | MS.gene74828:CDS | 60.0% | |
| CCCACAAAGGTCACCCCTTG+GGG | - | 23879:95474-95493 | MS.gene74828:CDS | 60.0% | |
| CCCCAAGGGGTGACCTTTGT+GGG | + | 23879:95477-95496 | None:intergenic | 60.0% | |
| TCCCCAAGGGGTGACCTTTG+TGG | + | 23879:95478-95497 | None:intergenic | 60.0% | |
| TTACCCATTTGCGGCGGCGA+CGG | + | 23879:95812-95831 | None:intergenic | 60.0% | |
| !! | CGGTTGGGAGGTGTCCATTG+GGG | + | 23879:95786-95805 | None:intergenic | 60.0% |
| !! | GCGGTTGGGAGGTGTCCATT+GGG | + | 23879:95787-95806 | None:intergenic | 60.0% |
| GGGGCTCCCCGCTTCATACT+TGG | - | 23879:95159-95178 | MS.gene74828:intron | 65.0% | |
| !! | GGCGGTTGGGAGGTGTCCAT+TGG | + | 23879:95788-95807 | None:intergenic | 65.0% |
| ATTTGCGGCGGCGACGGCGG+CGG | + | 23879:95806-95825 | None:intergenic | 75.0% | |
| CCCATTTGCGGCGGCGACGG+CGG | + | 23879:95809-95828 | None:intergenic | 75.0% | |
| CCGCCGTCGCCGCCGCAAAT+GGG | - | 23879:95806-95825 | MS.gene74828:CDS | 75.0% | |
| ! | GCCGCCGTCGCCGCCGCAAA+TGG | - | 23879:95805-95824 | MS.gene74828:CDS | 80.0% |
| ! | CGGCGACGGCGGCGGTTGGG+AGG | + | 23879:95798-95817 | None:intergenic | 85.0% |
| ! | CGGCGGCGACGGCGGCGGTT+GGG | + | 23879:95801-95820 | None:intergenic | 85.0% |
| ! | GCGGCGGCGACGGCGGCGGT+TGG | + | 23879:95802-95821 | None:intergenic | 90.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 23879 | gene | 95024 | 96507 | 95024 | ID=MS.gene74828 |
| 23879 | mRNA | 95024 | 96507 | 95024 | ID=MS.gene74828.t1;Parent=MS.gene74828 |
| 23879 | exon | 96351 | 96507 | 96351 | ID=MS.gene74828.t1.exon1;Parent=MS.gene74828.t1 |
| 23879 | CDS | 96351 | 96507 | 96351 | ID=cds.MS.gene74828.t1;Parent=MS.gene74828.t1 |
| 23879 | exon | 95704 | 96210 | 95704 | ID=MS.gene74828.t1.exon2;Parent=MS.gene74828.t1 |
| 23879 | CDS | 95704 | 96210 | 95704 | ID=cds.MS.gene74828.t1;Parent=MS.gene74828.t1 |
| 23879 | exon | 95412 | 95591 | 95412 | ID=MS.gene74828.t1.exon3;Parent=MS.gene74828.t1 |
| 23879 | CDS | 95412 | 95591 | 95412 | ID=cds.MS.gene74828.t1;Parent=MS.gene74828.t1 |
| 23879 | exon | 95240 | 95304 | 95240 | ID=MS.gene74828.t1.exon4;Parent=MS.gene74828.t1 |
| 23879 | CDS | 95240 | 95304 | 95240 | ID=cds.MS.gene74828.t1;Parent=MS.gene74828.t1 |
| 23879 | exon | 95024 | 95158 | 95024 | ID=MS.gene74828.t1.exon5;Parent=MS.gene74828.t1 |
| 23879 | CDS | 95024 | 95158 | 95024 | ID=cds.MS.gene74828.t1;Parent=MS.gene74828.t1 |
| Gene Sequence |
| Protein sequence |
