Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74830.t1 | AEX20345.1 | 67.6 | 401 | 63 | 3 | 1 | 341 | 66 | 459 | 1.40E-125 | 459.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74830.t1 | H2EIY5 | 67.6 | 401 | 63 | 3 | 1 | 341 | 66 | 459 | 9.7e-126 | 459.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05415 | MS.gene74830 | 0.815245 | 1.02E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74830.t1 | MTR_3g114180 | 83.969 | 131 | 19 | 1 | 216 | 346 | 7 | 135 | 1.47e-70 | 217 |
| MS.gene74830.t1 | MTR_3g114190 | 82.734 | 139 | 21 | 2 | 206 | 344 | 228 | 363 | 4.46e-69 | 221 |
| MS.gene74830.t1 | MTR_3g114190 | 85.271 | 129 | 17 | 1 | 216 | 344 | 7 | 133 | 9.21e-68 | 218 |
| MS.gene74830.t1 | MTR_3g113780 | 78.182 | 110 | 24 | 0 | 221 | 330 | 1 | 110 | 1.43e-55 | 177 |
| MS.gene74830.t1 | MTR_3g113780 | 76.744 | 86 | 20 | 0 | 221 | 306 | 1 | 86 | 4.74e-41 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAAATTTAATTAGAGA+CGG | + | 23879:113981-114000 | None:intergenic | 15.0% |
| !! | AAACACTACAAAAAAAAATG+TGG | + | 23879:113931-113950 | None:intergenic | 20.0% |
| !! | AACACTACAAAAAAAAATGT+GGG | + | 23879:113930-113949 | None:intergenic | 20.0% |
| !! | ATGAAATAATAAGAAACCAA+AGG | + | 23879:114597-114616 | None:intergenic | 20.0% |
| !! | TGAAATAATAAGAAACCAAA+GGG | + | 23879:114596-114615 | None:intergenic | 20.0% |
| !! | TTGGAATTTAATATTGTATC+TGG | - | 23879:114443-114462 | MS.gene74830:CDS | 20.0% |
| ! | AAAATTTACCAAGTATGAAG+CGG | + | 23879:113858-113877 | None:intergenic | 25.0% |
| ! | AAGAATACTTGAATGAGAAT+AGG | + | 23879:114535-114554 | None:intergenic | 25.0% |
| ! | TTTAAACTCAATTGAATAGC+AGG | + | 23879:113904-113923 | None:intergenic | 25.0% |
| ! | TTTAATTAGAGACGGAAAAA+AGG | + | 23879:113973-113992 | None:intergenic | 25.0% |
| !!! | AGATCTTAAGCATTTTTTAG+AGG | - | 23879:114013-114032 | MS.gene74830:intron | 25.0% |
| !!! | TTTTTGTAGTGTTTAGTTTG+AGG | - | 23879:113937-113956 | MS.gene74830:CDS | 25.0% |
| AAATTTACCAAGTATGAAGC+GGG | + | 23879:113857-113876 | None:intergenic | 30.0% | |
| ACTTGAATGAGAATAGGATT+AGG | + | 23879:114529-114548 | None:intergenic | 30.0% | |
| TAAGCAATATCCTAAAACTG+AGG | - | 23879:114109-114128 | MS.gene74830:CDS | 30.0% | |
| TTCTTTGACTGTTGTAATTC+TGG | - | 23879:114065-114084 | MS.gene74830:intron | 30.0% | |
| ! | AAAATGTAGAAAGCACAAAG+GGG | - | 23879:114664-114683 | MS.gene74830:CDS | 30.0% |
| ! | CAAAATGTAGAAAGCACAAA+GGG | - | 23879:114663-114682 | MS.gene74830:CDS | 30.0% |
| ! | GATCAAATTGATTTACAACC+TGG | - | 23879:114398-114417 | MS.gene74830:CDS | 30.0% |
| ! | GTTTCTTCTCTTGTTTCATA+TGG | - | 23879:114880-114899 | MS.gene74830:intron | 30.0% |
| !! | TAAGATCAAACCTTTGAAAC+TGG | - | 23879:114307-114326 | MS.gene74830:intron | 30.0% |
| !!! | AAAGTTATCGGTTTTTACCT+GGG | - | 23879:114253-114272 | MS.gene74830:CDS | 30.0% |
| !!! | TCTCTCTTTTTTTACATGTC+TGG | - | 23879:113763-113782 | MS.gene74830:CDS | 30.0% |
| !!! | TTTTTTTACATGTCTGGTCA+TGG | - | 23879:113769-113788 | MS.gene74830:CDS | 30.0% |
| AACTTCTTCATGAGAAGAAG+CGG | - | 23879:114634-114653 | MS.gene74830:CDS | 35.0% | |
| AATGGATCAGCTCTTTCAAA+TGG | - | 23879:113717-113736 | MS.gene74830:CDS | 35.0% | |
| AATTTACCAAGTATGAAGCG+GGG | + | 23879:113856-113875 | None:intergenic | 35.0% | |
| ACTGACAATCACAACACAAA+AGG | + | 23879:114424-114443 | None:intergenic | 35.0% | |
| AGACTTGGGATCAAAGTTAT+CGG | - | 23879:114241-114260 | MS.gene74830:CDS | 35.0% | |
| CAAATAATAACCTCACCCTT+TGG | - | 23879:114578-114597 | MS.gene74830:CDS | 35.0% | |
| GAAGAAACAAACATAAGCTG+AGG | + | 23879:114868-114887 | None:intergenic | 35.0% | |
| GATTTATAGACACTCCTTCT+TGG | + | 23879:114043-114062 | None:intergenic | 35.0% | |
| GCCAAAAGAATTTGAATACC+CGG | - | 23879:114764-114783 | MS.gene74830:CDS | 35.0% | |
| GCTGTTAGAAAGACATTGAT+CGG | + | 23879:115042-115061 | None:intergenic | 35.0% | |
| TAATAAGAAACCAAAGGGTG+AGG | + | 23879:114591-114610 | None:intergenic | 35.0% | |
| TAGATACAATAGACCTCAAG+TGG | - | 23879:114936-114955 | MS.gene74830:intron | 35.0% | |
| TTCTTGGACAAGAAGAGAAA+CGG | - | 23879:113793-113812 | MS.gene74830:CDS | 35.0% | |
| TTTCTAACAGCAAGCATACT+TGG | - | 23879:115051-115070 | MS.gene74830:CDS | 35.0% | |
| TTTGATCTTAGTCCCAAACT+CGG | + | 23879:114297-114316 | None:intergenic | 35.0% | |
| TTTGTGTTGTGATTGTCAGT+TGG | - | 23879:114424-114443 | MS.gene74830:CDS | 35.0% | |
| ! | AAATGTAGAAAGCACAAAGG+GGG | - | 23879:114665-114684 | MS.gene74830:CDS | 35.0% |
| ! | ACCGGGTATTCAAATTCTTT+TGG | + | 23879:114768-114787 | None:intergenic | 35.0% |
| ! | GCAAAATGTAGAAAGCACAA+AGG | - | 23879:114662-114681 | MS.gene74830:CDS | 35.0% |
| !! | AAAAAGAGCGTCTTGAGAAA+GGG | - | 23879:115149-115168 | MS.gene74830:CDS | 35.0% |
| !! | GCTATTTTCATGCAAGACTT+GGG | - | 23879:114227-114246 | MS.gene74830:CDS | 35.0% |
| !! | TTTTTAGAGGAAGTCCAAGA+AGG | - | 23879:114026-114045 | MS.gene74830:intron | 35.0% |
| !!! | AAAGAGAGAACCTCCTTTTT+TGG | + | 23879:113752-113771 | None:intergenic | 35.0% |
| !!! | CAAAGTTATCGGTTTTTACC+TGG | - | 23879:114252-114271 | MS.gene74830:CDS | 35.0% |
| !!! | TGCTATTTTCATGCAAGACT+TGG | - | 23879:114226-114245 | MS.gene74830:CDS | 35.0% |
| AGGTTGAGTTTACCCATTTG+CGG | + | 23879:114509-114528 | None:intergenic | 40.0% | |
| ATCAGCTCTTTCAAATGGCT+AGG | - | 23879:113722-113741 | MS.gene74830:CDS | 40.0% | |
| ATCTGTTCCACCAGTTTCAA+AGG | + | 23879:114320-114339 | None:intergenic | 40.0% | |
| CATACTTGGAGCATGAGAAA+AGG | - | 23879:115065-115084 | MS.gene74830:CDS | 40.0% | |
| CTTTCAACTTTCTGCTCCTT+TGG | + | 23879:114711-114730 | None:intergenic | 40.0% | |
| GAGGGAGAGTTTCTTTATGA+TGG | - | 23879:114735-114754 | MS.gene74830:CDS | 40.0% | |
| GTTTACGAAGAGACGTTGAA+AGG | - | 23879:114786-114805 | MS.gene74830:CDS | 40.0% | |
| TTCAACGTCTCTTCGTAAAC+CGG | + | 23879:114786-114805 | None:intergenic | 40.0% | |
| ! | ACTCTTCAAAAAGCATGCAG+AGG | - | 23879:115096-115115 | MS.gene74830:CDS | 40.0% |
| ! | ATCAGCATCCTTGTTGGTTA+TGG | - | 23879:113817-113836 | MS.gene74830:CDS | 40.0% |
| ! | GAAGCACAAGTTGACTTTGA+AGG | + | 23879:114143-114162 | None:intergenic | 40.0% |
| !! | CAGAAAGTTGAAAGCACTGA+GGG | - | 23879:114717-114736 | MS.gene74830:CDS | 40.0% |
| !! | GAAAAAGAGCGTCTTGAGAA+AGG | - | 23879:115148-115167 | MS.gene74830:CDS | 40.0% |
| !! | GATCAAACCTTTGAAACTGG+TGG | - | 23879:114310-114329 | MS.gene74830:intron | 40.0% |
| !! | GATTCTGCTTCGTTTTAGCA+TGG | + | 23879:114349-114368 | None:intergenic | 40.0% |
| AACGGAATCAGCATCCTTGT+TGG | - | 23879:113811-113830 | MS.gene74830:CDS | 45.0% | |
| AGAGAAAAACTCGCCTCATC+AGG | + | 23879:114974-114993 | None:intergenic | 45.0% | |
| AGCATGCAGAGGAAAAGAAG+CGG | - | 23879:115107-115126 | MS.gene74830:CDS | 45.0% | |
| ATCGCTACGGAATTGAAGGT+GGG | - | 23879:114371-114390 | MS.gene74830:intron | 45.0% | |
| ATGGCTAGGAAGGCCAAAAA+AGG | - | 23879:113736-113755 | MS.gene74830:CDS | 45.0% | |
| CAATCACAACACAAAAGGCC+AGG | + | 23879:114419-114438 | None:intergenic | 45.0% | |
| CATGTCTGGTCATGGATTCT+TGG | - | 23879:113777-113796 | MS.gene74830:CDS | 45.0% | |
| GAAGCAGAATCAGATCGCTA+CGG | - | 23879:114358-114377 | MS.gene74830:intron | 45.0% | |
| GCTCTTTCAAATGGCTAGGA+AGG | - | 23879:113726-113745 | MS.gene74830:CDS | 45.0% | |
| TACGGAATTGAAGGTGGGAA+TGG | - | 23879:114376-114395 | MS.gene74830:intron | 45.0% | |
| TATCCATGGGAAGAAGCCAA+AGG | - | 23879:114692-114711 | MS.gene74830:CDS | 45.0% | |
| TCAACGTCTCTTCGTAAACC+GGG | + | 23879:114785-114804 | None:intergenic | 45.0% | |
| TCAACTTGTGCTTCCCACAA+AGG | - | 23879:114149-114168 | MS.gene74830:CDS | 45.0% | |
| TCAGATCGCTACGGAATTGA+AGG | - | 23879:114367-114386 | MS.gene74830:intron | 45.0% | |
| TCCTGTACGTGAATGACATG+CGG | + | 23879:114198-114217 | None:intergenic | 45.0% | |
| TCTTCATGAGAAGAAGCGGA+AGG | - | 23879:114638-114657 | MS.gene74830:CDS | 45.0% | |
| TGCAAGTTCTCACCGAGTTT+GGG | - | 23879:114282-114301 | MS.gene74830:intron | 45.0% | |
| TTGAGAAAGGGAAGGGAAAG+AGG | - | 23879:115161-115180 | MS.gene74830:CDS | 45.0% | |
| TTGAGTTTACCCATTTGCGG+CGG | + | 23879:114506-114525 | None:intergenic | 45.0% | |
| ! | CATCCTTGTTGGTTATGGTG+AGG | - | 23879:113822-113841 | MS.gene74830:CDS | 45.0% |
| ! | CTTGTTGGTTATGGTGAGGA+CGG | - | 23879:113826-113845 | MS.gene74830:CDS | 45.0% |
| ! | GCACAAGTTGACTTTGAAGG+AGG | + | 23879:114140-114159 | None:intergenic | 45.0% |
| ! | GGAGGACTATCCTCAGTTTT+AGG | + | 23879:114122-114141 | None:intergenic | 45.0% |
| ! | TTGTTGGTTATGGTGAGGAC+GGG | - | 23879:113827-113846 | MS.gene74830:CDS | 45.0% |
| ! | TTTGGCTTCTTCCCATGGAT+AGG | + | 23879:114693-114712 | None:intergenic | 45.0% |
| !! | GCAGAAAGTTGAAAGCACTG+AGG | - | 23879:114716-114735 | MS.gene74830:CDS | 45.0% |
| ACAAAGGGGGATCCTATCCA+TGG | - | 23879:114678-114697 | MS.gene74830:CDS | 50.0% | |
| ACCGCATGTCATTCACGTAC+AGG | - | 23879:114194-114213 | MS.gene74830:CDS | 50.0% | |
| AGCAGACAGAGAGAGAGAGA+GGG | + | 23879:114825-114844 | None:intergenic | 50.0% | |
| ATGCAGAGGAAAAGAAGCGG+TGG | - | 23879:115110-115129 | MS.gene74830:CDS | 50.0% | |
| CAAAGGGGGATCCTATCCAT+GGG | - | 23879:114679-114698 | MS.gene74830:CDS | 50.0% | |
| CGTCCTCACCATAACCAACA+AGG | + | 23879:113828-113847 | None:intergenic | 50.0% | |
| GAGCGTCTTGAGAAAGGGAA+GGG | - | 23879:115154-115173 | MS.gene74830:CDS | 50.0% | |
| GATCGCTACGGAATTGAAGG+TGG | - | 23879:114370-114389 | MS.gene74830:intron | 50.0% | |
| GCTAGGAAGGCCAAAAAAGG+AGG | - | 23879:113739-113758 | MS.gene74830:CDS | 50.0% | |
| GTAGAACTGGACACCACTTG+AGG | + | 23879:114952-114971 | None:intergenic | 50.0% | |
| GTGCAAGTTCTCACCGAGTT+TGG | - | 23879:114281-114300 | MS.gene74830:intron | 50.0% | |
| GTGTCCAGTTCTACCTGATG+AGG | - | 23879:114958-114977 | MS.gene74830:intron | 50.0% | |
| TGTATCTGGACTCTCCCCAA+TGG | - | 23879:114457-114476 | MS.gene74830:CDS | 50.0% | |
| ! | AGAGCGTCTTGAGAAAGGGA+AGG | - | 23879:115153-115172 | MS.gene74830:CDS | 50.0% |
| ! | CTCTGTCTGCTGCTTGCATT+TGG | - | 23879:114834-114853 | MS.gene74830:CDS | 50.0% |
| ! | TGTTGGTTATGGTGAGGACG+GGG | - | 23879:113828-113847 | MS.gene74830:intron | 50.0% |
| AGACAGAGAGAGAGAGAGGG+AGG | + | 23879:114822-114841 | None:intergenic | 55.0% | |
| CAGCAGACAGAGAGAGAGAG+AGG | + | 23879:114826-114845 | None:intergenic | 55.0% | |
| CGGTAAATACCGTCCCCAAG+GGG | + | 23879:114178-114197 | None:intergenic | 55.0% | |
| CTCGCCTCATCAGGTAGAAC+TGG | + | 23879:114965-114984 | None:intergenic | 55.0% | |
| GACAGAGAGAGAGAGAGGGA+GGG | + | 23879:114821-114840 | None:intergenic | 55.0% | |
| GAGAACTTGCACAAGTGCCC+AGG | + | 23879:114273-114292 | None:intergenic | 55.0% | |
| GCGGTAAATACCGTCCCCAA+GGG | + | 23879:114179-114198 | None:intergenic | 55.0% | |
| TCCCACAAAGGTCACCCCTT+GGG | - | 23879:114161-114180 | MS.gene74830:CDS | 55.0% | |
| TGCGGTAAATACCGTCCCCA+AGG | + | 23879:114180-114199 | None:intergenic | 55.0% | |
| TTCCCACAAAGGTCACCCCT+TGG | - | 23879:114160-114179 | MS.gene74830:CDS | 55.0% | |
| ! | GCTCCTTTGGCTTCTTCCCA+TGG | + | 23879:114698-114717 | None:intergenic | 55.0% |
| CAAAGGTCACCCCTTGGGGA+CGG | - | 23879:114166-114185 | MS.gene74830:CDS | 60.0% | |
| CCCACAAAGGTCACCCCTTG+GGG | - | 23879:114162-114181 | MS.gene74830:CDS | 60.0% | |
| CCCCAAGGGGTGACCTTTGT+GGG | + | 23879:114165-114184 | None:intergenic | 60.0% | |
| TCCCCAAGGGGTGACCTTTG+TGG | + | 23879:114166-114185 | None:intergenic | 60.0% | |
| TTACCCATTTGCGGCGGCGA+CGG | + | 23879:114500-114519 | None:intergenic | 60.0% | |
| !! | CGGTTGGGAGGTGTCCATTG+GGG | + | 23879:114474-114493 | None:intergenic | 60.0% |
| !! | GCGGTTGGGAGGTGTCCATT+GGG | + | 23879:114475-114494 | None:intergenic | 60.0% |
| GGGGCTCCCCGCTTCATACT+TGG | - | 23879:113847-113866 | MS.gene74830:intron | 65.0% | |
| !! | GGCGGTTGGGAGGTGTCCAT+TGG | + | 23879:114476-114495 | None:intergenic | 65.0% |
| ATTTGCGGCGGCGACGGCGG+CGG | + | 23879:114494-114513 | None:intergenic | 75.0% | |
| CCCATTTGCGGCGGCGACGG+CGG | + | 23879:114497-114516 | None:intergenic | 75.0% | |
| CCGCCGTCGCCGCCGCAAAT+GGG | - | 23879:114494-114513 | MS.gene74830:CDS | 75.0% | |
| ! | GCCGCCGTCGCCGCCGCAAA+TGG | - | 23879:114493-114512 | MS.gene74830:CDS | 80.0% |
| ! | CGGCGACGGCGGCGGTTGGG+AGG | + | 23879:114486-114505 | None:intergenic | 85.0% |
| ! | CGGCGGCGACGGCGGCGGTT+GGG | + | 23879:114489-114508 | None:intergenic | 85.0% |
| ! | GCGGCGGCGACGGCGGCGGT+TGG | + | 23879:114490-114509 | None:intergenic | 90.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 23879 | gene | 113712 | 115195 | 113712 | ID=MS.gene74830 |
| 23879 | mRNA | 113712 | 115195 | 113712 | ID=MS.gene74830.t1;Parent=MS.gene74830 |
| 23879 | exon | 115039 | 115195 | 115039 | ID=MS.gene74830.t1.exon1;Parent=MS.gene74830.t1 |
| 23879 | CDS | 115039 | 115195 | 115039 | ID=cds.MS.gene74830.t1;Parent=MS.gene74830.t1 |
| 23879 | exon | 114392 | 114898 | 114392 | ID=MS.gene74830.t1.exon2;Parent=MS.gene74830.t1 |
| 23879 | CDS | 114392 | 114898 | 114392 | ID=cds.MS.gene74830.t1;Parent=MS.gene74830.t1 |
| 23879 | exon | 114100 | 114279 | 114100 | ID=MS.gene74830.t1.exon3;Parent=MS.gene74830.t1 |
| 23879 | CDS | 114100 | 114279 | 114100 | ID=cds.MS.gene74830.t1;Parent=MS.gene74830.t1 |
| 23879 | exon | 113928 | 113992 | 113928 | ID=MS.gene74830.t1.exon4;Parent=MS.gene74830.t1 |
| 23879 | CDS | 113928 | 113992 | 113928 | ID=cds.MS.gene74830.t1;Parent=MS.gene74830.t1 |
| 23879 | exon | 113712 | 113846 | 113712 | ID=MS.gene74830.t1.exon5;Parent=MS.gene74830.t1 |
| 23879 | CDS | 113712 | 113846 | 113712 | ID=cds.MS.gene74830.t1;Parent=MS.gene74830.t1 |
| Gene Sequence |
| Protein sequence |
