Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74832.t1 | AEX20345.1 | 67.6 | 401 | 63 | 3 | 1 | 341 | 66 | 459 | 1.40E-125 | 459.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74832.t1 | H2EIY5 | 67.6 | 401 | 63 | 3 | 1 | 341 | 66 | 459 | 9.7e-126 | 459.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05415 | MS.gene74832 | 0.815144 | 1.07E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74832.t1 | MTR_3g114180 | 83.969 | 131 | 19 | 1 | 216 | 346 | 7 | 135 | 1.47e-70 | 217 |
| MS.gene74832.t1 | MTR_3g114190 | 82.734 | 139 | 21 | 2 | 206 | 344 | 228 | 363 | 4.46e-69 | 221 |
| MS.gene74832.t1 | MTR_3g114190 | 85.271 | 129 | 17 | 1 | 216 | 344 | 7 | 133 | 9.21e-68 | 218 |
| MS.gene74832.t1 | MTR_3g113780 | 78.182 | 110 | 24 | 0 | 221 | 330 | 1 | 110 | 1.43e-55 | 177 |
| MS.gene74832.t1 | MTR_3g113780 | 76.744 | 86 | 20 | 0 | 221 | 306 | 1 | 86 | 4.74e-41 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 133 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAACAAATTTAATTAGAGA+CGG | + | 23879:129111-129130 | None:intergenic | 15.0% |
| !! | AAACACTACAAAAAAAAATG+TGG | + | 23879:129061-129080 | None:intergenic | 20.0% |
| !! | AACACTACAAAAAAAAATGT+GGG | + | 23879:129060-129079 | None:intergenic | 20.0% |
| !! | ATGAAATAATAAGAAACCAA+AGG | + | 23879:129727-129746 | None:intergenic | 20.0% |
| !! | TGAAATAATAAGAAACCAAA+GGG | + | 23879:129726-129745 | None:intergenic | 20.0% |
| !! | TTGGAATTTAATATTGTATC+TGG | - | 23879:129573-129592 | MS.gene74832:CDS | 20.0% |
| ! | AAAATTTACCAAGTATGAAG+CGG | + | 23879:128988-129007 | None:intergenic | 25.0% |
| ! | AAGAATACTTGAATGAGAAT+AGG | + | 23879:129665-129684 | None:intergenic | 25.0% |
| ! | TTTAAACTCAATTGAATAGC+AGG | + | 23879:129034-129053 | None:intergenic | 25.0% |
| ! | TTTAATTAGAGACGGAAAAA+AGG | + | 23879:129103-129122 | None:intergenic | 25.0% |
| !!! | AGATCTTAAGCATTTTTTAG+AGG | - | 23879:129143-129162 | MS.gene74832:intron | 25.0% |
| !!! | TTTTTGTAGTGTTTAGTTTG+AGG | - | 23879:129067-129086 | MS.gene74832:CDS | 25.0% |
| AAATTTACCAAGTATGAAGC+GGG | + | 23879:128987-129006 | None:intergenic | 30.0% | |
| ACTTGAATGAGAATAGGATT+AGG | + | 23879:129659-129678 | None:intergenic | 30.0% | |
| TAAGCAATATCCTAAAACTG+AGG | - | 23879:129239-129258 | MS.gene74832:CDS | 30.0% | |
| TTCTTTGACTGTTGTAATTC+TGG | - | 23879:129195-129214 | MS.gene74832:intron | 30.0% | |
| ! | AAAATGTAGAAAGCACAAAG+GGG | - | 23879:129794-129813 | MS.gene74832:CDS | 30.0% |
| ! | CAAAATGTAGAAAGCACAAA+GGG | - | 23879:129793-129812 | MS.gene74832:CDS | 30.0% |
| ! | GATCAAATTGATTTACAACC+TGG | - | 23879:129528-129547 | MS.gene74832:CDS | 30.0% |
| ! | GTTTCTTCTCTTGTTTCATA+TGG | - | 23879:130010-130029 | MS.gene74832:intron | 30.0% |
| !! | TAAGATCAAACCTTTGAAAC+TGG | - | 23879:129437-129456 | MS.gene74832:intron | 30.0% |
| !!! | AAAGTTATCGGTTTTTACCT+GGG | - | 23879:129383-129402 | MS.gene74832:CDS | 30.0% |
| !!! | TCTCTCTTTTTTTACATGTC+TGG | - | 23879:128893-128912 | MS.gene74832:CDS | 30.0% |
| !!! | TTTTTTTACATGTCTGGTCA+TGG | - | 23879:128899-128918 | MS.gene74832:CDS | 30.0% |
| AACTTCTTCATGAGAAGAAG+CGG | - | 23879:129764-129783 | MS.gene74832:CDS | 35.0% | |
| AATGGATCAGCTCTTTCAAA+TGG | - | 23879:128847-128866 | MS.gene74832:CDS | 35.0% | |
| AATTTACCAAGTATGAAGCG+GGG | + | 23879:128986-129005 | None:intergenic | 35.0% | |
| ACTGACAATCACAACACAAA+AGG | + | 23879:129554-129573 | None:intergenic | 35.0% | |
| AGACTTGGGATCAAAGTTAT+CGG | - | 23879:129371-129390 | MS.gene74832:CDS | 35.0% | |
| CAAATAATAACCTCACCCTT+TGG | - | 23879:129708-129727 | MS.gene74832:CDS | 35.0% | |
| GAAGAAACAAACATAAGCTG+AGG | + | 23879:129998-130017 | None:intergenic | 35.0% | |
| GATTTATAGACACTCCTTCT+TGG | + | 23879:129173-129192 | None:intergenic | 35.0% | |
| GCCAAAAGAATTTGAATACC+CGG | - | 23879:129894-129913 | MS.gene74832:CDS | 35.0% | |
| GCTGTTAGAAAGACATTGAT+CGG | + | 23879:130172-130191 | None:intergenic | 35.0% | |
| TAATAAGAAACCAAAGGGTG+AGG | + | 23879:129721-129740 | None:intergenic | 35.0% | |
| TAGATACAATAGACCTCAAG+TGG | - | 23879:130066-130085 | MS.gene74832:intron | 35.0% | |
| TTCTTGGACAAGAAGAGAAA+CGG | - | 23879:128923-128942 | MS.gene74832:CDS | 35.0% | |
| TTTCTAACAGCAAGCATACT+TGG | - | 23879:130181-130200 | MS.gene74832:CDS | 35.0% | |
| TTTGATCTTAGTCCCAAACT+CGG | + | 23879:129427-129446 | None:intergenic | 35.0% | |
| TTTGTGTTGTGATTGTCAGT+TGG | - | 23879:129554-129573 | MS.gene74832:CDS | 35.0% | |
| ! | AAATGTAGAAAGCACAAAGG+GGG | - | 23879:129795-129814 | MS.gene74832:CDS | 35.0% |
| ! | ACCGGGTATTCAAATTCTTT+TGG | + | 23879:129898-129917 | None:intergenic | 35.0% |
| ! | GCAAAATGTAGAAAGCACAA+AGG | - | 23879:129792-129811 | MS.gene74832:CDS | 35.0% |
| !! | AAAAAGAGCGTCTTGAGAAA+GGG | - | 23879:130279-130298 | MS.gene74832:CDS | 35.0% |
| !! | GCTATTTTCATGCAAGACTT+GGG | - | 23879:129357-129376 | MS.gene74832:CDS | 35.0% |
| !! | TTTTTAGAGGAAGTCCAAGA+AGG | - | 23879:129156-129175 | MS.gene74832:intron | 35.0% |
| !!! | AAAGAGAGAACCTCCTTTTT+TGG | + | 23879:128882-128901 | None:intergenic | 35.0% |
| !!! | CAAAGTTATCGGTTTTTACC+TGG | - | 23879:129382-129401 | MS.gene74832:CDS | 35.0% |
| !!! | TGCTATTTTCATGCAAGACT+TGG | - | 23879:129356-129375 | MS.gene74832:CDS | 35.0% |
| AGGTTGAGTTTACCCATTTG+CGG | + | 23879:129639-129658 | None:intergenic | 40.0% | |
| ATCAGCTCTTTCAAATGGCT+AGG | - | 23879:128852-128871 | MS.gene74832:CDS | 40.0% | |
| ATCTGTTCCACCAGTTTCAA+AGG | + | 23879:129450-129469 | None:intergenic | 40.0% | |
| CATACTTGGAGCATGAGAAA+AGG | - | 23879:130195-130214 | MS.gene74832:CDS | 40.0% | |
| CTTTCAACTTTCTGCTCCTT+TGG | + | 23879:129841-129860 | None:intergenic | 40.0% | |
| GAGGGAGAGTTTCTTTATGA+TGG | - | 23879:129865-129884 | MS.gene74832:CDS | 40.0% | |
| GTTTACGAAGAGACGTTGAA+AGG | - | 23879:129916-129935 | MS.gene74832:CDS | 40.0% | |
| TTCAACGTCTCTTCGTAAAC+CGG | + | 23879:129916-129935 | None:intergenic | 40.0% | |
| ! | ACTCTTCAAAAAGCATGCAG+AGG | - | 23879:130226-130245 | MS.gene74832:CDS | 40.0% |
| ! | ATCAGCATCCTTGTTGGTTA+TGG | - | 23879:128947-128966 | MS.gene74832:CDS | 40.0% |
| ! | GAAGCACAAGTTGACTTTGA+AGG | + | 23879:129273-129292 | None:intergenic | 40.0% |
| !! | CAGAAAGTTGAAAGCACTGA+GGG | - | 23879:129847-129866 | MS.gene74832:CDS | 40.0% |
| !! | GAAAAAGAGCGTCTTGAGAA+AGG | - | 23879:130278-130297 | MS.gene74832:CDS | 40.0% |
| !! | GATCAAACCTTTGAAACTGG+TGG | - | 23879:129440-129459 | MS.gene74832:intron | 40.0% |
| !! | GATTCTGCTTCGTTTTAGCA+TGG | + | 23879:129479-129498 | None:intergenic | 40.0% |
| AACGGAATCAGCATCCTTGT+TGG | - | 23879:128941-128960 | MS.gene74832:CDS | 45.0% | |
| AGAGAAAAACTCGCCTCATC+AGG | + | 23879:130104-130123 | None:intergenic | 45.0% | |
| AGCATGCAGAGGAAAAGAAG+CGG | - | 23879:130237-130256 | MS.gene74832:CDS | 45.0% | |
| ATCGCTACGGAATTGAAGGT+GGG | - | 23879:129501-129520 | MS.gene74832:intron | 45.0% | |
| ATGGCTAGGAAGGCCAAAAA+AGG | - | 23879:128866-128885 | MS.gene74832:CDS | 45.0% | |
| CAATCACAACACAAAAGGCC+AGG | + | 23879:129549-129568 | None:intergenic | 45.0% | |
| CATGTCTGGTCATGGATTCT+TGG | - | 23879:128907-128926 | MS.gene74832:CDS | 45.0% | |
| GAAGCAGAATCAGATCGCTA+CGG | - | 23879:129488-129507 | MS.gene74832:intron | 45.0% | |
| GCTCTTTCAAATGGCTAGGA+AGG | - | 23879:128856-128875 | MS.gene74832:CDS | 45.0% | |
| TACGGAATTGAAGGTGGGAA+TGG | - | 23879:129506-129525 | MS.gene74832:intron | 45.0% | |
| TATCCATGGGAAGAAGCCAA+AGG | - | 23879:129822-129841 | MS.gene74832:CDS | 45.0% | |
| TCAACGTCTCTTCGTAAACC+GGG | + | 23879:129915-129934 | None:intergenic | 45.0% | |
| TCAACTTGTGCTTCCCACAA+AGG | - | 23879:129279-129298 | MS.gene74832:CDS | 45.0% | |
| TCAGATCGCTACGGAATTGA+AGG | - | 23879:129497-129516 | MS.gene74832:intron | 45.0% | |
| TCCTGTACGTGAATGACATG+CGG | + | 23879:129328-129347 | None:intergenic | 45.0% | |
| TCTTCATGAGAAGAAGCGGA+AGG | - | 23879:129768-129787 | MS.gene74832:CDS | 45.0% | |
| TGCAAGTTCTCACCGAGTTT+GGG | - | 23879:129412-129431 | MS.gene74832:intron | 45.0% | |
| TTGAGAAAGGGAAGGGAAAG+AGG | - | 23879:130291-130310 | MS.gene74832:CDS | 45.0% | |
| TTGAGTTTACCCATTTGCGG+CGG | + | 23879:129636-129655 | None:intergenic | 45.0% | |
| ! | CATCCTTGTTGGTTATGGTG+AGG | - | 23879:128952-128971 | MS.gene74832:CDS | 45.0% |
| ! | CTTGTTGGTTATGGTGAGGA+CGG | - | 23879:128956-128975 | MS.gene74832:CDS | 45.0% |
| ! | GCACAAGTTGACTTTGAAGG+AGG | + | 23879:129270-129289 | None:intergenic | 45.0% |
| ! | GGAGGACTATCCTCAGTTTT+AGG | + | 23879:129252-129271 | None:intergenic | 45.0% |
| ! | TTGTTGGTTATGGTGAGGAC+GGG | - | 23879:128957-128976 | MS.gene74832:CDS | 45.0% |
| ! | TTTGGCTTCTTCCCATGGAT+AGG | + | 23879:129823-129842 | None:intergenic | 45.0% |
| !! | GCAGAAAGTTGAAAGCACTG+AGG | - | 23879:129846-129865 | MS.gene74832:CDS | 45.0% |
| ACAAAGGGGGATCCTATCCA+TGG | - | 23879:129808-129827 | MS.gene74832:CDS | 50.0% | |
| ACCGCATGTCATTCACGTAC+AGG | - | 23879:129324-129343 | MS.gene74832:CDS | 50.0% | |
| AGCAGACAGAGAGAGAGAGA+GGG | + | 23879:129955-129974 | None:intergenic | 50.0% | |
| ATGCAGAGGAAAAGAAGCGG+TGG | - | 23879:130240-130259 | MS.gene74832:CDS | 50.0% | |
| CAAAGGGGGATCCTATCCAT+GGG | - | 23879:129809-129828 | MS.gene74832:CDS | 50.0% | |
| CGTCCTCACCATAACCAACA+AGG | + | 23879:128958-128977 | None:intergenic | 50.0% | |
| GAGCGTCTTGAGAAAGGGAA+GGG | - | 23879:130284-130303 | MS.gene74832:CDS | 50.0% | |
| GATCGCTACGGAATTGAAGG+TGG | - | 23879:129500-129519 | MS.gene74832:intron | 50.0% | |
| GCTAGGAAGGCCAAAAAAGG+AGG | - | 23879:128869-128888 | MS.gene74832:CDS | 50.0% | |
| GTAGAACTGGACACCACTTG+AGG | + | 23879:130082-130101 | None:intergenic | 50.0% | |
| GTGCAAGTTCTCACCGAGTT+TGG | - | 23879:129411-129430 | MS.gene74832:intron | 50.0% | |
| GTGTCCAGTTCTACCTGATG+AGG | - | 23879:130088-130107 | MS.gene74832:intron | 50.0% | |
| TGTATCTGGACTCTCCCCAA+TGG | - | 23879:129587-129606 | MS.gene74832:CDS | 50.0% | |
| ! | AGAGCGTCTTGAGAAAGGGA+AGG | - | 23879:130283-130302 | MS.gene74832:CDS | 50.0% |
| ! | CTCTGTCTGCTGCTTGCATT+TGG | - | 23879:129964-129983 | MS.gene74832:CDS | 50.0% |
| ! | TGTTGGTTATGGTGAGGACG+GGG | - | 23879:128958-128977 | MS.gene74832:intron | 50.0% |
| AGACAGAGAGAGAGAGAGGG+AGG | + | 23879:129952-129971 | None:intergenic | 55.0% | |
| CAGCAGACAGAGAGAGAGAG+AGG | + | 23879:129956-129975 | None:intergenic | 55.0% | |
| CGGTAAATACCGTCCCCAAG+GGG | + | 23879:129308-129327 | None:intergenic | 55.0% | |
| CTCGCCTCATCAGGTAGAAC+TGG | + | 23879:130095-130114 | None:intergenic | 55.0% | |
| GACAGAGAGAGAGAGAGGGA+GGG | + | 23879:129951-129970 | None:intergenic | 55.0% | |
| GAGAACTTGCACAAGTGCCC+AGG | + | 23879:129403-129422 | None:intergenic | 55.0% | |
| GCGGTAAATACCGTCCCCAA+GGG | + | 23879:129309-129328 | None:intergenic | 55.0% | |
| TCCCACAAAGGTCACCCCTT+GGG | - | 23879:129291-129310 | MS.gene74832:CDS | 55.0% | |
| TGCGGTAAATACCGTCCCCA+AGG | + | 23879:129310-129329 | None:intergenic | 55.0% | |
| TTCCCACAAAGGTCACCCCT+TGG | - | 23879:129290-129309 | MS.gene74832:CDS | 55.0% | |
| ! | GCTCCTTTGGCTTCTTCCCA+TGG | + | 23879:129828-129847 | None:intergenic | 55.0% |
| CAAAGGTCACCCCTTGGGGA+CGG | - | 23879:129296-129315 | MS.gene74832:CDS | 60.0% | |
| CCCACAAAGGTCACCCCTTG+GGG | - | 23879:129292-129311 | MS.gene74832:CDS | 60.0% | |
| CCCCAAGGGGTGACCTTTGT+GGG | + | 23879:129295-129314 | None:intergenic | 60.0% | |
| TCCCCAAGGGGTGACCTTTG+TGG | + | 23879:129296-129315 | None:intergenic | 60.0% | |
| TTACCCATTTGCGGCGGCGA+CGG | + | 23879:129630-129649 | None:intergenic | 60.0% | |
| !! | CGGTTGGGAGGTGTCCATTG+GGG | + | 23879:129604-129623 | None:intergenic | 60.0% |
| !! | GCGGTTGGGAGGTGTCCATT+GGG | + | 23879:129605-129624 | None:intergenic | 60.0% |
| GGGGCTCCCCGCTTCATACT+TGG | - | 23879:128977-128996 | MS.gene74832:intron | 65.0% | |
| !! | GGCGGTTGGGAGGTGTCCAT+TGG | + | 23879:129606-129625 | None:intergenic | 65.0% |
| ATTTGCGGCGGCGACGGCGG+CGG | + | 23879:129624-129643 | None:intergenic | 75.0% | |
| CCCATTTGCGGCGGCGACGG+CGG | + | 23879:129627-129646 | None:intergenic | 75.0% | |
| CCGCCGTCGCCGCCGCAAAT+GGG | - | 23879:129624-129643 | MS.gene74832:CDS | 75.0% | |
| ! | GCCGCCGTCGCCGCCGCAAA+TGG | - | 23879:129623-129642 | MS.gene74832:CDS | 80.0% |
| ! | CGGCGACGGCGGCGGTTGGG+AGG | + | 23879:129616-129635 | None:intergenic | 85.0% |
| ! | CGGCGGCGACGGCGGCGGTT+GGG | + | 23879:129619-129638 | None:intergenic | 85.0% |
| ! | GCGGCGGCGACGGCGGCGGT+TGG | + | 23879:129620-129639 | None:intergenic | 90.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 23879 | gene | 128842 | 130325 | 128842 | ID=MS.gene74832 |
| 23879 | mRNA | 128842 | 130325 | 128842 | ID=MS.gene74832.t1;Parent=MS.gene74832 |
| 23879 | exon | 130169 | 130325 | 130169 | ID=MS.gene74832.t1.exon1;Parent=MS.gene74832.t1 |
| 23879 | CDS | 130169 | 130325 | 130169 | ID=cds.MS.gene74832.t1;Parent=MS.gene74832.t1 |
| 23879 | exon | 129522 | 130028 | 129522 | ID=MS.gene74832.t1.exon2;Parent=MS.gene74832.t1 |
| 23879 | CDS | 129522 | 130028 | 129522 | ID=cds.MS.gene74832.t1;Parent=MS.gene74832.t1 |
| 23879 | exon | 129230 | 129409 | 129230 | ID=MS.gene74832.t1.exon3;Parent=MS.gene74832.t1 |
| 23879 | CDS | 129230 | 129409 | 129230 | ID=cds.MS.gene74832.t1;Parent=MS.gene74832.t1 |
| 23879 | exon | 129058 | 129122 | 129058 | ID=MS.gene74832.t1.exon4;Parent=MS.gene74832.t1 |
| 23879 | CDS | 129058 | 129122 | 129058 | ID=cds.MS.gene74832.t1;Parent=MS.gene74832.t1 |
| 23879 | exon | 128842 | 128976 | 128842 | ID=MS.gene74832.t1.exon5;Parent=MS.gene74832.t1 |
| 23879 | CDS | 128842 | 128976 | 128842 | ID=cds.MS.gene74832.t1;Parent=MS.gene74832.t1 |
| Gene Sequence |
| Protein sequence |
