AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75135

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75135.t1 MTR_1g115230 93.007 143 10 0 9 151 68 210 3.38e-90 278
MS.gene75135.t1 MTR_1g115230 92.453 53 4 0 150 202 422 474 2.89e-25 102
MS.gene75135.t1 MTR_5g048280 31.250 144 94 3 13 153 116 257 2.57e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75135.t1 AT1G50380 71.528 144 41 0 9 152 75 218 7.86e-69 223
MS.gene75135.t1 AT1G50380 79.245 53 11 0 150 202 429 481 2.69e-20 88.6
MS.gene75135.t1 AT1G50380 71.528 144 41 0 9 152 141 284 1.98e-68 223
MS.gene75135.t1 AT1G50380 79.245 53 11 0 150 202 495 547 2.99e-20 88.6
MS.gene75135.t1 AT1G69020 30.233 172 107 4 10 174 107 272 2.24e-11 62.8

Find 46 sgRNAs with CRISPR-Local

Find 122 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGCTACTAACTTGTTATT+TGG 0.087936 1.1:+80843655 None:intergenic
TCAGATCCATTACTACTTTA+TGG 0.129739 1.1:-80842584 MS.gene75135:CDS
TTACTATTATACGAAAATTT+TGG 0.202008 1.1:-80843958 MS.gene75135:CDS
TCCTCCTGAGCATGTTATTT+TGG 0.223944 1.1:-80843841 MS.gene75135:CDS
ATGCTACTAACTTGTTATTT+GGG 0.325588 1.1:+80843656 None:intergenic
AAAGATCTTGCGAAGCTTGA+TGG 0.328021 1.1:-80842608 MS.gene75135:CDS
AGTATATATTTCATCTCCTT+TGG 0.347787 1.1:+80843621 None:intergenic
TCCTATTGTTCTCAGGTTCT+CGG 0.373350 1.1:-80842719 MS.gene75135:intron
CATTAACAGATGGAACCTTT+TGG 0.374692 1.1:+80843889 None:intergenic
GGAGAATTTCCCACCTTGTC+AGG 0.392612 1.1:+80843475 None:intergenic
ATCGAGACTCAAGCTGCAAT+TGG 0.433413 1.1:-80843583 MS.gene75135:CDS
ACATGCTCAGGAGGAGCATC+AGG 0.440289 1.1:+80843849 None:intergenic
GTGTAACATCAAACCTTGAA+TGG 0.445714 1.1:-80843546 MS.gene75135:CDS
CCATTACTACTTTATGGCTA+CGG 0.485771 1.1:-80842578 MS.gene75135:CDS
TCATCCAAAATAACATGCTC+AGG 0.488078 1.1:+80843837 None:intergenic
CATTACTACTTTATGGCTAC+GGG 0.491993 1.1:-80842577 MS.gene75135:CDS
CCGTAGCCATAAAGTAGTAA+TGG 0.504218 1.1:+80842578 None:intergenic
CATCACCACCAGCCCATTCA+AGG 0.505524 1.1:+80843533 None:intergenic
GGAAAATGCTTACACCGATT+CGG 0.512108 1.1:-80844803 None:intergenic
TACACCGATTCGGTCATGTC+AGG 0.514534 1.1:-80844793 MS.gene75135:intron
GTTAATGACACCATGCCTAC+AGG 0.526408 1.1:-80843873 MS.gene75135:CDS
GATACCTGACATGACCGAAT+CGG 0.533607 1.1:+80844789 None:intergenic
AATATATACTGTTTATGTCA+TGG 0.538740 1.1:-80843608 MS.gene75135:CDS
TGTAACATCAAACCTTGAAT+GGG 0.542086 1.1:-80843545 MS.gene75135:CDS
TAATACGACCCTTTGCGTAC+AGG 0.547949 1.1:+80843978 None:intergenic
TTCAAGGTTTGATGTTACAC+TGG 0.548154 1.1:+80843549 None:intergenic
CCGTATATCCGATAACCAAA+AGG 0.550075 1.1:-80843904 MS.gene75135:CDS
CTTTATGGCTACGGGTCTTG+AGG 0.555022 1.1:-80842569 None:intergenic
AGCATCAGGTCCTGTAGGCA+TGG 0.564851 1.1:+80843863 None:intergenic
CAGTGGGCACATGCTTCAGA+TGG 0.568505 1.1:-80842662 MS.gene75135:CDS
TAACTGCACCTGTACGCAAA+GGG 0.575768 1.1:-80843986 MS.gene75135:CDS
CAACAAAATACGTCACTGAA+AGG 0.580218 1.1:-80842685 MS.gene75135:CDS
TGCTCTAGTTTATGTAACAA+TGG 0.586588 1.1:-80843512 MS.gene75135:CDS
AATACGTCACTGAAAGGCAG+TGG 0.587769 1.1:-80842679 MS.gene75135:CDS
ACATCAAACCTTGAATGGGC+TGG 0.602081 1.1:-80843541 MS.gene75135:CDS
GGAGGAGCATCAGGTCCTGT+AGG 0.606439 1.1:+80843858 None:intergenic
CAGCACGAATACTACAGTAT+CGG 0.612278 1.1:-80843798 MS.gene75135:CDS
TTTGATGTTACACTGGCAAG+AGG 0.621915 1.1:+80843556 None:intergenic
GGCATGGTGTCATTAACAGA+TGG 0.625215 1.1:+80843879 None:intergenic
ATAACTGCACCTGTACGCAA+AGG 0.628634 1.1:-80843987 MS.gene75135:CDS
TCCAAAATAACATGCTCAGG+AGG 0.646141 1.1:+80843840 None:intergenic
TAATATTCTTAGACCTGACA+AGG 0.648307 1.1:-80843488 MS.gene75135:intron
ATACGTCACTGAAAGGCAGT+GGG 0.650078 1.1:-80842678 MS.gene75135:CDS
GCATATGCAGAAGACACCAA+AGG 0.654059 1.1:-80843637 MS.gene75135:CDS
TATTGTTCTCAGGTTCTCGG+AGG 0.667662 1.1:-80842716 MS.gene75135:intron
TCAAACCTTGAATGGGCTGG+TGG 0.679492 1.1:-80843538 MS.gene75135:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTACTATTATAACTATTAT+AGG - chr1.1:80844571-80844590 MS.gene75135:intron 10.0%
!!! TTAAAATTTTATTTCTTTTC+AGG - chr1.1:80843686-80843705 MS.gene75135:intron 10.0%
!! TTTAGAAAAACTATAACAAA+CGG - chr1.1:80843067-80843086 MS.gene75135:intron 15.0%
!!! AATTCAATGATATCAATTTT+TGG + chr1.1:80844509-80844528 None:intergenic 15.0%
!!! TTACTATTATACGAAAATTT+TGG - chr1.1:80843411-80843430 MS.gene75135:intron 15.0%
!!! TTTTCAATTTCAATTTGTTT+AGG - chr1.1:80844151-80844170 MS.gene75135:intron 15.0%
!! AAACAGATTATTAAAACCAT+TGG + chr1.1:80842837-80842856 None:intergenic 20.0%
!! AATATATACTGTTTATGTCA+TGG - chr1.1:80843761-80843780 MS.gene75135:intron 20.0%
!! TTAGAAAAACTATAACAAAC+GGG - chr1.1:80843068-80843087 MS.gene75135:intron 20.0%
!! TTAGATCATATCATTATCAT+GGG - chr1.1:80843605-80843624 MS.gene75135:CDS 20.0%
!!! AATTAGGTTCTAATTCAATT+CGG + chr1.1:80844188-80844207 None:intergenic 20.0%
!!! ATACACATTTTTCATTTTCT+AGG - chr1.1:80843351-80843370 MS.gene75135:intron 20.0%
!!! ATTATACGAAAATTTTGGAA+GGG - chr1.1:80843416-80843435 MS.gene75135:intron 20.0%
!!! TATTATACGAAAATTTTGGA+AGG - chr1.1:80843415-80843434 MS.gene75135:intron 20.0%
!!! TGTTAAAAAAGTTGATAAGA+TGG + chr1.1:80844040-80844059 None:intergenic 20.0%
! AAATTAGAAGATGAGATTTG+TGG - chr1.1:80844230-80844249 MS.gene75135:intron 25.0%
! AGTATATATTTCATCTCCTT+TGG + chr1.1:80843751-80843770 None:intergenic 25.0%
! GTTAGATCATATCATTATCA+TGG - chr1.1:80843604-80843623 MS.gene75135:CDS 25.0%
! TCAAAAAAGTCGATCTAAAT+AGG - chr1.1:80843111-80843130 MS.gene75135:intron 25.0%
! TCTTTATATCTGGCTAAATT+AGG + chr1.1:80844204-80844223 None:intergenic 25.0%
! TTTGAACTCATTGTACAAAT+GGG - chr1.1:80843197-80843216 MS.gene75135:intron 25.0%
!! ATTTTCAGCATCTTTATATC+TGG + chr1.1:80844214-80844233 None:intergenic 25.0%
!!! ATGCTACTAACTTGTTATTT+GGG + chr1.1:80843716-80843735 None:intergenic 25.0%
!!! TAAAAAAGTTGATAAGATGG+AGG + chr1.1:80844037-80844056 None:intergenic 25.0%
!!! TATCTACTTGAGCTTTTTTA+AGG - chr1.1:80844333-80844352 MS.gene75135:intron 25.0%
!!! TATGCTACTAACTTGTTATT+TGG + chr1.1:80843717-80843736 None:intergenic 25.0%
!!! TATTTTGGATGAGAATATCA+AGG - chr1.1:80843543-80843562 MS.gene75135:CDS 25.0%
AAATTGAAAACATTGCCCAA+GGG + chr1.1:80844141-80844160 None:intergenic 30.0%
AACGAAAACTAGTTACTGTT+AGG + chr1.1:80842777-80842796 None:intergenic 30.0%
AGATCCGAACAATTAAATAC+TGG + chr1.1:80843927-80843946 None:intergenic 30.0%
AGTCAAATCATGAAAAGCTT+TGG + chr1.1:80843986-80844005 None:intergenic 30.0%
CAACCCAGTATTTAATTGTT+CGG - chr1.1:80843920-80843939 MS.gene75135:CDS 30.0%
CAATAGGAGACAAATAATAC+AGG + chr1.1:80844638-80844657 None:intergenic 30.0%
CGATCTAAATAGGTTCAATA+TGG - chr1.1:80843121-80843140 MS.gene75135:intron 30.0%
GATCCGAACAATTAAATACT+GGG + chr1.1:80843926-80843945 None:intergenic 30.0%
GTTTGAACTCATTGTACAAA+TGG - chr1.1:80843196-80843215 MS.gene75135:intron 30.0%
TAATATTCTTAGACCTGACA+AGG - chr1.1:80843881-80843900 MS.gene75135:CDS 30.0%
TCAGATCCATTACTACTTTA+TGG - chr1.1:80844785-80844804 MS.gene75135:intron 30.0%
TGAGCTTATCTACTGAAATA+CGG + chr1.1:80843094-80843113 None:intergenic 30.0%
TGTAACATCAAACCTTGAAT+GGG - chr1.1:80843824-80843843 MS.gene75135:CDS 30.0%
! CAATTAAATACTGGGTTGAA+AGG + chr1.1:80843918-80843937 None:intergenic 30.0%
! CACATTTTTCATTTTCTAGG+AGG - chr1.1:80843354-80843373 MS.gene75135:intron 30.0%
! TGCTCTAGTTTATGTAACAA+TGG - chr1.1:80843857-80843876 MS.gene75135:CDS 30.0%
! TTTTCTGCTTAATTCAAAGC+CGG - chr1.1:80844291-80844310 MS.gene75135:intron 30.0%
!!! AGAAGGCTTTTGAAATTTGA+AGG - chr1.1:80843299-80843318 MS.gene75135:intron 30.0%
!!! ATTGCTTCTTTTTTTCCCAA+AGG - chr1.1:80844526-80844545 MS.gene75135:intron 30.0%
AAATTACTCCACAAGTCCTT+TGG + chr1.1:80844545-80844564 None:intergenic 35.0%
AAGCAGAAAACATCCAGTTT+CGG + chr1.1:80844281-80844300 None:intergenic 35.0%
AATTACTCCACAAGTCCTTT+GGG + chr1.1:80844544-80844563 None:intergenic 35.0%
AGGATCATGAATCGATTGTT+TGG + chr1.1:80844095-80844114 None:intergenic 35.0%
ATATATACACACGCTTCAGA+AGG - chr1.1:80843282-80843301 MS.gene75135:intron 35.0%
ATCAGTCAGAAACTTATGTC+CGG + chr1.1:80844313-80844332 None:intergenic 35.0%
ATTGTTTGGACATAGGAGAA+AGG + chr1.1:80844081-80844100 None:intergenic 35.0%
ATTTGTCTCCTATTGTTCTC+AGG - chr1.1:80844643-80844662 MS.gene75135:intron 35.0%
CAACAAAATACGTCACTGAA+AGG - chr1.1:80844684-80844703 MS.gene75135:intron 35.0%
CATTACTACTTTATGGCTAC+GGG - chr1.1:80844792-80844811 MS.gene75135:intron 35.0%
CCATTACTACTTTATGGCTA+CGG - chr1.1:80844791-80844810 MS.gene75135:intron 35.0%
GAAATTGAAAACATTGCCCA+AGG + chr1.1:80844142-80844161 None:intergenic 35.0%
GAATGTGTAATTCAATGCAC+AGG - chr1.1:80842707-80842726 MS.gene75135:CDS 35.0%
GAGCAATTGAACATAACTCA+AGG + chr1.1:80844609-80844628 None:intergenic 35.0%
GCGTGTGTATATATAGCAAT+AGG + chr1.1:80843276-80843295 None:intergenic 35.0%
GTGTAACATCAAACCTTGAA+TGG - chr1.1:80843823-80843842 MS.gene75135:CDS 35.0%
TCATCCAAAATAACATGCTC+AGG + chr1.1:80843535-80843554 None:intergenic 35.0%
TGAATCGATTGTTTGGACAT+AGG + chr1.1:80844088-80844107 None:intergenic 35.0%
TGTAATTCAATGCACAGGTT+TGG - chr1.1:80842712-80842731 MS.gene75135:intron 35.0%
TTCAAGGTTTGATGTTACAC+TGG + chr1.1:80843823-80843842 None:intergenic 35.0%
! CATTAACAGATGGAACCTTT+TGG + chr1.1:80843483-80843502 None:intergenic 35.0%
!! AAAAGCATTGAAAGCGAAAG+AGG + chr1.1:80842630-80842649 None:intergenic 35.0%
!! AGATGGAACCTTTTGGTTAT+CGG + chr1.1:80843476-80843495 None:intergenic 35.0%
!! CCTTTTGGTTATCGGATATA+CGG + chr1.1:80843468-80843487 None:intergenic 35.0%
!! TACGAAAATTTTGGAAGGGA+AGG - chr1.1:80843420-80843439 MS.gene75135:intron 35.0%
!!! CTACAGTATCGGTACTTTTA+AGG - chr1.1:80843582-80843601 MS.gene75135:CDS 35.0%
!!! GTTCTGTAGCTGCTATTTTT+TGG + chr1.1:80844474-80844493 None:intergenic 35.0%
!!! TTTTTTTCCCAAAGGACTTG+TGG - chr1.1:80844534-80844553 MS.gene75135:intron 35.0%
AAAGATCTTGCGAAGCTTGA+TGG - chr1.1:80844761-80844780 MS.gene75135:intron 40.0%
ATTCTTAGACCTGACAAGGT+GGG - chr1.1:80843885-80843904 MS.gene75135:CDS 40.0%
CAGCACGAATACTACAGTAT+CGG - chr1.1:80843571-80843590 MS.gene75135:CDS 40.0%
CCGTAGCCATAAAGTAGTAA+TGG + chr1.1:80844794-80844813 None:intergenic 40.0%
CCGTATATCCGATAACCAAA+AGG - chr1.1:80843465-80843484 MS.gene75135:intron 40.0%
GAACATAACTCAAGGAAACG+AGG + chr1.1:80844601-80844620 None:intergenic 40.0%
GAACGATCATGAAGAGCTTA+TGG - chr1.1:80842959-80842978 MS.gene75135:intron 40.0%
TATTCTTAGACCTGACAAGG+TGG - chr1.1:80843884-80843903 MS.gene75135:CDS 40.0%
TCCAAAATAACATGCTCAGG+AGG + chr1.1:80843532-80843551 None:intergenic 40.0%
TCCTATTGTTCTCAGGTTCT+CGG - chr1.1:80844650-80844669 MS.gene75135:intron 40.0%
TGAATATGCGAGAAGACGTT+AGG + chr1.1:80844115-80844134 None:intergenic 40.0%
TTTCAATCTGCAACCGAAAC+TGG - chr1.1:80844265-80844284 MS.gene75135:intron 40.0%
TTTGATGTTACACTGGCAAG+AGG + chr1.1:80843816-80843835 None:intergenic 40.0%
! ATGACTGTAACATGCTGACT+AGG - chr1.1:80843246-80843265 MS.gene75135:intron 40.0%
! CAAGCTTCGCAAGATCTTTT+CGG + chr1.1:80844760-80844779 None:intergenic 40.0%
! TTCTAGAAGCTGAACCAGTT+CGG + chr1.1:80842754-80842773 None:intergenic 40.0%
!! TCCTCCTGAGCATGTTATTT+TGG - chr1.1:80843528-80843547 MS.gene75135:CDS 40.0%
AATACGTCACTGAAAGGCAG+TGG - chr1.1:80844690-80844709 MS.gene75135:intron 45.0%
ACATCAAACCTTGAATGGGC+TGG - chr1.1:80843828-80843847 MS.gene75135:CDS 45.0%
ATAACTGCACCTGTACGCAA+AGG - chr1.1:80843382-80843401 MS.gene75135:intron 45.0%
ATACGTCACTGAAAGGCAGT+GGG - chr1.1:80844691-80844710 MS.gene75135:intron 45.0%
ATCGAGACTCAAGCTGCAAT+TGG - chr1.1:80843786-80843805 MS.gene75135:intron 45.0%
CTCACAAATGAACTCGCCAA+TGG - chr1.1:80842818-80842837 MS.gene75135:intron 45.0%
CTCGCATATTCAAAGCCCTT+GGG - chr1.1:80844123-80844142 MS.gene75135:intron 45.0%
GATACCTGACATGACCGAAT+CGG + chr1.1:80842583-80842602 None:intergenic 45.0%
GCAGCTACAGAACAGAATGA+AGG - chr1.1:80844481-80844500 MS.gene75135:intron 45.0%
GCATATGCAGAAGACACCAA+AGG - chr1.1:80843732-80843751 MS.gene75135:intron 45.0%
GTTAATGACACCATGCCTAC+AGG - chr1.1:80843496-80843515 MS.gene75135:CDS 45.0%
TAACTGCACCTGTACGCAAA+GGG - chr1.1:80843383-80843402 MS.gene75135:intron 45.0%
TAATACGACCCTTTGCGTAC+AGG + chr1.1:80843394-80843413 None:intergenic 45.0%
TATTGTTCTCAGGTTCTCGG+AGG - chr1.1:80844653-80844672 MS.gene75135:intron 45.0%
TCAGAATTTCCGAACCGAAC+TGG - chr1.1:80842737-80842756 MS.gene75135:intron 45.0%
TCCGAGAACCTGAGAACAAT+AGG + chr1.1:80844654-80844673 None:intergenic 45.0%
TCTCGCATATTCAAAGCCCT+TGG - chr1.1:80844122-80844141 MS.gene75135:intron 45.0%
!! GGCATGGTGTCATTAACAGA+TGG + chr1.1:80843493-80843512 None:intergenic 45.0%
!!! ATTTTAAATAAAATAAAAAC+AGG + chr1.1:80843673-80843692 None:intergenic 5.0%
!!! TTAAGATTTTTTTTTAATTA+AGG + chr1.1:80844369-80844388 None:intergenic 5.0%
!!! TTTTAAATAAAATAAAAACA+GGG + chr1.1:80843672-80843691 None:intergenic 5.0%
GAAGCTGAACCAGTTCGGTT+CGG + chr1.1:80842749-80842768 None:intergenic 50.0%
GGAGAATTTCCCACCTTGTC+AGG + chr1.1:80843897-80843916 None:intergenic 50.0%
TCACAGTGTTGCAAGTTGCG+TGG + chr1.1:80842685-80842704 None:intergenic 50.0%
!! TACACCGATTCGGTCATGTC+AGG - chr1.1:80842576-80842595 MS.gene75135:CDS 50.0%
!! TCAAACCTTGAATGGGCTGG+TGG - chr1.1:80843831-80843850 MS.gene75135:CDS 50.0%
AGCATCAGGTCCTGTAGGCA+TGG + chr1.1:80843509-80843528 None:intergenic 55.0%
CAGTGGGCACATGCTTCAGA+TGG - chr1.1:80844707-80844726 MS.gene75135:intron 55.0%
CATCACCACCAGCCCATTCA+AGG + chr1.1:80843839-80843858 None:intergenic 55.0%
!! ACATGCTCAGGAGGAGCATC+AGG + chr1.1:80843523-80843542 None:intergenic 55.0%
!! GGAGGAGCATCAGGTCCTGT+AGG + chr1.1:80843514-80843533 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 80842571 80844820 80842571 ID=MS.gene75135
chr1.1 mRNA 80842571 80844820 80842571 ID=MS.gene75135.t1;Parent=MS.gene75135
chr1.1 exon 80844794 80844820 80844794 ID=MS.gene75135.t1.exon1;Parent=MS.gene75135.t1
chr1.1 CDS 80844794 80844820 80844794 ID=cds.MS.gene75135.t1;Parent=MS.gene75135.t1
chr1.1 exon 80843788 80844018 80843788 ID=MS.gene75135.t1.exon2;Parent=MS.gene75135.t1
chr1.1 CDS 80843788 80844018 80843788 ID=cds.MS.gene75135.t1;Parent=MS.gene75135.t1
chr1.1 exon 80843489 80843683 80843489 ID=MS.gene75135.t1.exon3;Parent=MS.gene75135.t1
chr1.1 CDS 80843489 80843683 80843489 ID=cds.MS.gene75135.t1;Parent=MS.gene75135.t1
chr1.1 exon 80842571 80842726 80842571 ID=MS.gene75135.t1.exon4;Parent=MS.gene75135.t1
chr1.1 CDS 80842571 80842726 80842571 ID=cds.MS.gene75135.t1;Parent=MS.gene75135.t1
Gene Sequence

>MS.gene75135

ATGCTTACACCGATTCGGTCATGTCAGGTATCAATAATTCTATTTCATTATCTTATCCTCTTTCGCTTTCAATGCTTTTTCATTTATTATTAATTATTTATAAAATACGATCCACGCAACTTGCAACACTGTGAACGAATGTGTAATTCAATGCACAGGTTTGGTTTCAGAATTTCCGAACCGAACTGGTTCAGCTTCTAGAACCTAACAGTAACTAGTTTTCGTTTTAAGAACTCAACTGCTTCTACTCACAAATGAACTCGCCAATGGTTTTAATAATCTGTTTCGATTAAACAGCTTATTTTGTATAAGCGCAAGCGCTTATGCATAAGTTATTTCTATAAAATAAAGTCATATTGCTTACGTATATTCTATAAGTTGTTTTCACGAACGATCATGAAGAGCTTATGGAAATAAGCTGAAAACAGCTCATGAACGTCTCATTTGAAGCTTTCTTAAAAAATTATAGCAAAGCATGATACACTATAAAAATCTTTTTAGAAAAACTATAACAAACGGGCCGTATTTCAGTAGATAAGCTCAAAAAAGTCGATCTAAATAGGTTCAATATGGCATTGTTAAGTAAACACTATAGATTAGACACAACTAAACTGATCAAGCATATGTTTGAACTCATTGTACAAATGGGTAGAGACTCAATGACGTAGAGACTCAATGACTGTAACATGCTGACTAGGAAATCCTATTGCTATATATACACACGCTTCAGAAGGCTTTTGAAATTTGAAGGAGATTAATTCAAATCACTAGCATAATATTATACACATTTTTCATTTTCTAGGAGGAAGATATAACTGCACCTGTACGCAAAGGGTCGTATTACTATTATACGAAAATTTTGGAAGGGAAGGAGTATGTTCAACATTGTAGACGCCGTATATCCGATAACCAAAAGGTTCCATCTGTTAATGACACCATGCCTACAGGACCTGATGCTCCTCCTGAGCATGTTATTTTGGATGAGAATATCAAGGCACAACAGCACGAATACTACAGTATCGGTACTTTTAAGGTTAGATCATATCATTATCATGGGATTTTAATTTTGTTTTATTCTCTTTTGTTTCTTGATTTCTGCCCTGTTTTTATTTTATTTAAAATTTTATTTCTTTTCAGGTTAGCCCAAATAACAAGTTAGTAGCATATGCAGAAGACACCAAAGGAGATGAAATATATACTGTTTATGTCATGGATATCGAGACTCAAGCTGCAATTGGAGAGCCTCTTGCCAGTGTAACATCAAACCTTGAATGGGCTGGTGGTGATGCTCTAGTTTATGTAACAATGGATAATATTCTTAGACCTGACAAGGTGGGAAATTCTCCTTTCAACCCAGTATTTAATTGTTCGGATCTAATCAGCACTTCTTTATTCAGAAACAATAACAACAGCCAAAGCTTTTCATGATTTGACTAAGAGTCTTTCATGATTCAACTTTGTATCCTCCATCTTATCAACTTTTTTAACAACTACGAAAACTTTTTTTCCTTTCTCCTATGTCCAAACAATCGATTCATGATCCTAACGTCTTCTCGCATATTCAAAGCCCTTGGGCAATGTTTTCAATTTCAATTTGTTTAGGAGCGAAACTTACCGAATTGAATTAGAACCTAATTTAGCCAGATATAAAGATGCTGAAAATTAGAAGATGAGATTTGTGGTTACAGTGTGTGTTTCAATCTGCAACCGAAACTGGATGTTTTCTGCTTAATTCAAAGCCGGACATAAGTTTCTGACTGATTATCTACTTGAGCTTTTTTAAGGAAAAATTGCACCTTAATTAAAAAAAAATCTTAATAAGTTTTATAGTAATTCAGTAAGTTTTTTCAGAAATAAGCAAAACTCTCAAACAAAGTTGTTTTAATGCTTTTCTTGTAAGCCAAAAAATAGCAGCTACAGAACAGAATGAAGGATCCAAAAATTGATATCATTGAATTGCTTCTTTTTTTCCCAAAGGACTTGTGGAGTAATTTAATCTAATTACTATTATAACTATTATAGGTTTTCCTCGTTTCCTTGAGTTATGTTCAATTGCTCTCTATACCTGTATTATTTGTCTCCTATTGTTCTCAGGTTCTCGGAGGTTTTGATGCAACAAAATACGTCACTGAAAGGCAGTGGGCACATGCTTCAGATGGAACTTTGATTCTCATGTCACTTGTATACCGAAAAGATCTTGCGAAGCTTGATGGATCAGATCCATTACTACTTTATGGCTACGGGTCTTGA
Protein sequence

>MS.gene75135.t1

MLTPIRSCQEEDITAPVRKGSYYYYTKILEGKEYVQHCRRRISDNQKVPSVNDTMPTGPDAPPEHVILDENIKAQQHEYYSIGTFKVSPNNKLVAYAEDTKGDEIYTVYVMDIETQAAIGEPLASVTSNLEWAGGDALVYVTMDNILRPDKVLGGFDATKYVTERQWAHASDGTLILMSLVYRKDLAKLDGSDPLLLYGYGS