Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75428.t1 | KYP31853.1 | 47.8 | 274 | 19 | 2 | 1 | 151 | 1042 | 1314 | 7.10E-55 | 223.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75428.t1 | P10978 | 36.9 | 268 | 46 | 1 | 2 | 146 | 1058 | 1325 | 6.5e-41 | 168.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene75428.t1 | A0A151QNH6 | 47.8 | 274 | 19 | 2 | 1 | 151 | 1042 | 1314 | 5.1e-55 | 223.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058881 | MS.gene75428 | 0.840017 | 1.12E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACTATCCTTTGATTAGTTT+GGG | 0.219958 | 3.3:-73335826 | None:intergenic |
| AAACTATCCTTTGATTAGTT+TGG | 0.231148 | 3.3:-73335827 | None:intergenic |
| TGCTCATCTCTTCTTTCTCT+TGG | 0.271304 | 3.3:-73335142 | None:intergenic |
| CAAAACTCTATCTTCTCTTT+TGG | 0.321800 | 3.3:-73335776 | None:intergenic |
| TCTTTCTCTTGGTCATTCTC+AGG | 0.376250 | 3.3:-73335131 | None:intergenic |
| GCTACCCACTGCTGAAGAAT+AGG | 0.395959 | 3.3:-73335171 | None:intergenic |
| ACATCGATGTGAGGTATCAT+TGG | 0.435433 | 3.3:+73335666 | MS.gene75428:intron |
| CATATCTGCTCCATTGTCAT+TGG | 0.455158 | 3.3:-73335738 | None:intergenic |
| AACACTGGAGTGCTGTGAAA+TGG | 0.463543 | 3.3:+73335279 | MS.gene75428:CDS |
| CCTGTGCATGTTCCGCTACC+TGG | 0.472224 | 3.3:+73335080 | MS.gene75428:CDS |
| CTACCCACTGCTGAAGAATA+GGG | 0.473406 | 3.3:-73335170 | None:intergenic |
| TGCAGACCATGGCGTACATA+AGG | 0.500035 | 3.3:-73335193 | None:intergenic |
| TTGTAAAGAAGCTGCAGGCT+TGG | 0.530111 | 3.3:+73335796 | MS.gene75428:CDS |
| ATCTCAAGAAAGTTCTTGAA+AGG | 0.540106 | 3.3:+73335036 | MS.gene75428:CDS |
| CCTACAGCATAACCGATGTC+TGG | 0.542961 | 3.3:-73335221 | None:intergenic |
| CCAGACATCGGTTATGCTGT+AGG | 0.556943 | 3.3:+73335221 | MS.gene75428:CDS |
| GTCTGGCCTCGTGCAGACCA+TGG | 0.563338 | 3.3:-73335204 | None:intergenic |
| CCAGGTAGCGGAACATGCAC+AGG | 0.572979 | 3.3:-73335080 | None:intergenic |
| TCATTGGATACGTGATGTGT+TGG | 0.576055 | 3.3:+73335682 | MS.gene75428:CDS |
| AAAATTCATACCAATGACAA+TGG | 0.582534 | 3.3:+73335728 | MS.gene75428:CDS |
| TTACTGAGTTTCATATGACC+AGG | 0.584762 | 3.3:-73335098 | None:intergenic |
| GTGCCCTATTCTTCAGCAGT+GGG | 0.600613 | 3.3:+73335167 | MS.gene75428:CDS |
| GGTGCCCTATTCTTCAGCAG+TGG | 0.613159 | 3.3:+73335166 | MS.gene75428:CDS |
| ACTATCCTTTGATTAGTTTG+GGG | 0.614975 | 3.3:-73335825 | None:intergenic |
| GGGTAGCCTTATGTACGCCA+TGG | 0.620781 | 3.3:+73335187 | MS.gene75428:CDS |
| CAAACCCAGGAAAAGAACAC+TGG | 0.623987 | 3.3:+73335264 | MS.gene75428:CDS |
| AGAGAAAGAAGAGATGAGCA+AGG | 0.630245 | 3.3:+73335145 | MS.gene75428:CDS |
| GTCTGCACGAGGCCAGACAT+CGG | 0.643214 | 3.3:+73335209 | MS.gene75428:CDS |
| ATCGTTCAAGAAGAATGTTG+TGG | 0.658412 | 3.3:+73334997 | None:intergenic |
| AGTAGATTCCTATCAAACCC+AGG | 0.660954 | 3.3:+73335251 | MS.gene75428:CDS |
| AGTTTCATATGACCAGGTAG+CGG | 0.678545 | 3.3:-73335092 | None:intergenic |
| TGTACGCCATGGTCTGCACG+AGG | 0.697121 | 3.3:+73335198 | MS.gene75428:CDS |
| GTGCTGTGAAATGGATACTG+AGG | 0.727600 | 3.3:+73335288 | MS.gene75428:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAAATTCATACCAATGACAA+TGG | + | chr3.3:73335728-73335747 | MS.gene75428:CDS | 25.0% |
| ! | TGCAAAAGTAATCAAATATC+CGG | - | chr3.3:73335426-73335445 | None:intergenic | 25.0% |
| !!! | ATATTTGATTACTTTTGCAG+GGG | + | chr3.3:73335427-73335446 | MS.gene75428:intron | 25.0% |
| !!! | GATATTTGATTACTTTTGCA+GGG | + | chr3.3:73335426-73335445 | MS.gene75428:intron | 25.0% |
| ATCTCAAGAAAGTTCTTGAA+AGG | + | chr3.3:73335036-73335055 | MS.gene75428:CDS | 30.0% | |
| GAACATATCTTTCTTGATTC+TGG | - | chr3.3:73335580-73335599 | None:intergenic | 30.0% | |
| ! | CAAAACTCTATCTTCTCTTT+TGG | - | chr3.3:73335779-73335798 | None:intergenic | 30.0% |
| ! | TTTTCTTGAATCTACATCTC+CGG | - | chr3.3:73335396-73335415 | None:intergenic | 30.0% |
| !! | GGATATTTGATTACTTTTGC+AGG | + | chr3.3:73335425-73335444 | MS.gene75428:intron | 30.0% |
| !! | TATTTGATTACTTTTGCAGG+GGG | + | chr3.3:73335428-73335447 | MS.gene75428:intron | 30.0% |
| AAAAAGTTCCTGCAAGAGTT+AGG | + | chr3.3:73335554-73335573 | MS.gene75428:intron | 35.0% | |
| AGTAATCAAATATCCGGATG+TGG | - | chr3.3:73335420-73335439 | None:intergenic | 35.0% | |
| GATTCAAGAAAATCCACATC+CGG | + | chr3.3:73335404-73335423 | MS.gene75428:intron | 35.0% | |
| TTACTGAGTTTCATATGACC+AGG | - | chr3.3:73335101-73335120 | None:intergenic | 35.0% | |
| ! | GCCAAAACATAAGCACTTTT+TGG | - | chr3.3:73335330-73335349 | None:intergenic | 35.0% |
| ! | TGGATACTGAGGTATCTAAA+AGG | + | chr3.3:73335299-73335318 | MS.gene75428:intron | 35.0% |
| !!! | AAGTGCTTATGTTTTGGCAA+TGG | + | chr3.3:73335332-73335351 | MS.gene75428:intron | 35.0% |
| !!! | GCCAAAAAGTGCTTATGTTT+TGG | + | chr3.3:73335326-73335345 | MS.gene75428:intron | 35.0% |
| AATGGAAATCAAATGCTCGC+TGG | + | chr3.3:73335350-73335369 | MS.gene75428:intron | 40.0% | |
| AGAACTCGACATACCATTCA+AGG | + | chr3.3:73335636-73335655 | MS.gene75428:intron | 40.0% | |
| AGAGAAAGAAGAGATGAGCA+AGG | + | chr3.3:73335145-73335164 | MS.gene75428:CDS | 40.0% | |
| AGTAGATTCCTATCAAACCC+AGG | + | chr3.3:73335251-73335270 | MS.gene75428:CDS | 40.0% | |
| AGTTTCATATGACCAGGTAG+CGG | - | chr3.3:73335095-73335114 | None:intergenic | 40.0% | |
| CATATCTGCTCCATTGTCAT+TGG | - | chr3.3:73335741-73335760 | None:intergenic | 40.0% | |
| TCATTGGATACGTGATGTGT+TGG | + | chr3.3:73335682-73335701 | MS.gene75428:CDS | 40.0% | |
| TCTTTCTCTTGGTCATTCTC+AGG | - | chr3.3:73335134-73335153 | None:intergenic | 40.0% | |
| TGCTCATCTCTTCTTTCTCT+TGG | - | chr3.3:73335145-73335164 | None:intergenic | 40.0% | |
| TGGTTACGTAGATGCTGATA+TGG | + | chr3.3:73335370-73335389 | MS.gene75428:intron | 40.0% | |
| ! | ACATCGATGTGAGGTATCAT+TGG | + | chr3.3:73335666-73335685 | MS.gene75428:intron | 40.0% |
| ! | GTTCTTTTCCTGGGTTTGAT+AGG | - | chr3.3:73335262-73335281 | None:intergenic | 40.0% |
| !! | GAGTTTTGTAAAGAAGCTGC+AGG | + | chr3.3:73335791-73335810 | MS.gene75428:CDS | 40.0% |
| AAGGTTGTAAGGAGATCCTG+TGG | + | chr3.3:73335528-73335547 | MS.gene75428:intron | 45.0% | |
| CAAACCCAGGAAAAGAACAC+TGG | + | chr3.3:73335264-73335283 | MS.gene75428:CDS | 45.0% | |
| CGATGTGTTTCGACCTTGAA+TGG | - | chr3.3:73335652-73335671 | None:intergenic | 45.0% | |
| CGCAATCACTGAAGGTTGTA+AGG | + | chr3.3:73335517-73335536 | MS.gene75428:intron | 45.0% | |
| CTACCCACTGCTGAAGAATA+GGG | - | chr3.3:73335173-73335192 | None:intergenic | 45.0% | |
| GAGTACATCGCAATCACTGA+AGG | + | chr3.3:73335509-73335528 | MS.gene75428:intron | 45.0% | |
| GTGCTGTGAAATGGATACTG+AGG | + | chr3.3:73335288-73335307 | MS.gene75428:CDS | 45.0% | |
| TACGTAGATGCTGATATGGC+CGG | + | chr3.3:73335374-73335393 | MS.gene75428:intron | 45.0% | |
| TGCTGAGGTGAATTGCACTT+TGG | - | chr3.3:73335616-73335635 | None:intergenic | 45.0% | |
| TTGTAAAGAAGCTGCAGGCT+TGG | + | chr3.3:73335796-73335815 | MS.gene75428:CDS | 45.0% | |
| ! | CACTCCAGTGTTCTTTTCCT+GGG | - | chr3.3:73335271-73335290 | None:intergenic | 45.0% |
| !! | AACACTGGAGTGCTGTGAAA+TGG | + | chr3.3:73335279-73335298 | MS.gene75428:CDS | 45.0% |
| !!! | GCAGGAACTTTTTCATCCAC+AGG | - | chr3.3:73335547-73335566 | None:intergenic | 45.0% |
| CCTACAGCATAACCGATGTC+TGG | - | chr3.3:73335224-73335243 | None:intergenic | 50.0% | |
| GATTCTGGCCTAACTCTTGC+AGG | - | chr3.3:73335565-73335584 | None:intergenic | 50.0% | |
| GCTACCCACTGCTGAAGAAT+AGG | - | chr3.3:73335174-73335193 | None:intergenic | 50.0% | |
| GGTATGTCGAGTTCTTGCTG+AGG | - | chr3.3:73335631-73335650 | None:intergenic | 50.0% | |
| GGTCGAAACACATCGATGTG+AGG | + | chr3.3:73335657-73335676 | MS.gene75428:intron | 50.0% | |
| GTGCCCTATTCTTCAGCAGT+GGG | + | chr3.3:73335167-73335186 | MS.gene75428:CDS | 50.0% | |
| TGCAGACCATGGCGTACATA+AGG | - | chr3.3:73335196-73335215 | None:intergenic | 50.0% | |
| TGCGATGTACTCTGCTTCTG+TGG | - | chr3.3:73335501-73335520 | None:intergenic | 50.0% | |
| ! | CCAGACATCGGTTATGCTGT+AGG | + | chr3.3:73335221-73335240 | MS.gene75428:CDS | 50.0% |
| ! | GCACTCCAGTGTTCTTTTCC+TGG | - | chr3.3:73335272-73335291 | None:intergenic | 50.0% |
| GGTGCCCTATTCTTCAGCAG+TGG | + | chr3.3:73335166-73335185 | MS.gene75428:CDS | 55.0% | |
| !! | GGGTAGCCTTATGTACGCCA+TGG | + | chr3.3:73335187-73335206 | MS.gene75428:CDS | 55.0% |
| CCTGTGCATGTTCCGCTACC+TGG | + | chr3.3:73335080-73335099 | MS.gene75428:CDS | 60.0% | |
| GTCTGCACGAGGCCAGACAT+CGG | + | chr3.3:73335209-73335228 | MS.gene75428:CDS | 60.0% | |
| TGTACGCCATGGTCTGCACG+AGG | + | chr3.3:73335198-73335217 | MS.gene75428:CDS | 60.0% | |
| TTGCAGGGGGAGCTGTCTCA+TGG | + | chr3.3:73335441-73335460 | MS.gene75428:intron | 60.0% | |
| !! | CCAGGTAGCGGAACATGCAC+AGG | - | chr3.3:73335083-73335102 | None:intergenic | 60.0% |
| GTCTGGCCTCGTGCAGACCA+TGG | - | chr3.3:73335207-73335226 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 73335011 | 73335835 | 73335011 | ID=MS.gene75428 |
| chr3.3 | mRNA | 73335011 | 73335835 | 73335011 | ID=MS.gene75428.t1;Parent=MS.gene75428 |
| chr3.3 | exon | 73335011 | 73335309 | 73335011 | ID=MS.gene75428.t1.exon1;Parent=MS.gene75428.t1 |
| chr3.3 | CDS | 73335011 | 73335309 | 73335011 | ID=cds.MS.gene75428.t1;Parent=MS.gene75428.t1 |
| chr3.3 | exon | 73335679 | 73335835 | 73335679 | ID=MS.gene75428.t1.exon2;Parent=MS.gene75428.t1 |
| chr3.3 | CDS | 73335679 | 73335835 | 73335679 | ID=cds.MS.gene75428.t1;Parent=MS.gene75428.t1 |
| Gene Sequence |
| Protein sequence |
