AlfalfaGEDB | MS.gene75546

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75546.t1	XP_028184779.1	38.7	142	79	3	2	143	185	318	1.90E-17	99

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene75546.t1	A0A2K3LDL8	43.9	148	72	5	1	144	234	374	1.4e-17	99.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene058515	MS.gene75546	0.843668	1.22E-58	-1.69E-46

PPI:

Gene1	Gene2	Type

Find 24 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAGTCTCCCTTGTACTTTC+TGG	0.230884	3.1:-69103406	None:intergenic
CCTCACCTTCAAAAGACTTT+TGG	0.258427	3.1:+69103543	MS.gene75546:CDS
AAGTACAAGGGAGACTCTTC+TGG	0.273875	3.1:+69103411	MS.gene75546:CDS
AACTGCACATGCTTCTAATC+AGG	0.343665	3.1:+69103725	MS.gene75546:CDS
GTTTCTTCTTCCATGTCTCT+TGG	0.385064	3.1:+69103747	MS.gene75546:CDS
ACGTACACCATGTTTAGAAT+TGG	0.414531	3.1:-69103454	None:intergenic
ATCACACTGTGGAAACTTGT+TGG	0.471826	3.1:+69103502	MS.gene75546:CDS
TATTGCTCATGTGTAATAGC+TGG	0.484097	3.1:-69103651	None:intergenic
TGCTGATTCCAGAAAGTACA+AGG	0.500741	3.1:+69103398	MS.gene75546:CDS
TTGGTCATTCACCAACTGAC+AGG	0.539995	3.1:+69103766	MS.gene75546:CDS
TGCTAATCATATTGCAAAGG+AGG	0.567084	3.1:+69103575	MS.gene75546:CDS
CAAGGAACCAATTCTAAACA+TGG	0.567547	3.1:+69103447	MS.gene75546:CDS
TCTGGAAAATCACAATCTCA+AGG	0.572777	3.1:+69103429	MS.gene75546:CDS
CTAGTACCTTGCCTGTCAGT+TGG	0.575846	3.1:-69103777	None:intergenic
ATCTGCTAATCATATTGCAA+AGG	0.593639	3.1:+69103572	MS.gene75546:CDS
CAGAATTCATCTCTTAACCA+AGG	0.602810	3.1:+69103696	MS.gene75546:CDS
GAAACTTGTTGGCAGAAACA+TGG	0.603043	3.1:+69103513	MS.gene75546:CDS
TGGTGAATGACCAAGAGACA+TGG	0.623399	3.1:-69103757	None:intergenic
ATCAAAACTGAATCTTCACT+AGG	0.653799	3.1:-69103369	None:intergenic
CATTCACCAACTGACAGGCA+AGG	0.674940	3.1:+69103771	MS.gene75546:CDS
GTACGTGTGTGTACTTTCTG+TGG	0.678321	3.1:+69103471	MS.gene75546:CDS
GATGAGAAGTCTTCATCATG+TGG	0.706859	3.1:+69103609	MS.gene75546:CDS
GCTGATTCCAGAAAGTACAA+GGG	0.713667	3.1:+69103399	MS.gene75546:CDS
TGGTAGAAAGAATCACACTG+TGG	0.768352	3.1:+69103491	MS.gene75546:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATCAAAACTGAATCTTCACT+AGG	-	chr3.1:69103372-69103391	None:intergenic	30.0%
	ATCTGCTAATCATATTGCAA+AGG	+	chr3.1:69103572-69103591	MS.gene75546:CDS	30.0%
!	AAGAACCAAAAGTCTTTTGA+AGG	-	chr3.1:69103551-69103570	None:intergenic	30.0%
	CAAGGAACCAATTCTAAACA+TGG	+	chr3.1:69103447-69103466	MS.gene75546:CDS	35.0%
	CAGAATTCATCTCTTAACCA+AGG	+	chr3.1:69103696-69103715	MS.gene75546:CDS	35.0%
	TATTGCTCATGTGTAATAGC+TGG	-	chr3.1:69103654-69103673	None:intergenic	35.0%
	TCTGGAAAATCACAATCTCA+AGG	+	chr3.1:69103429-69103448	MS.gene75546:CDS	35.0%
	TGCTAATCATATTGCAAAGG+AGG	+	chr3.1:69103575-69103594	MS.gene75546:CDS	35.0%
!	ACGTACACCATGTTTAGAAT+TGG	-	chr3.1:69103457-69103476	None:intergenic	35.0%
	AACTGCACATGCTTCTAATC+AGG	+	chr3.1:69103725-69103744	MS.gene75546:CDS	40.0%
	ATCACACTGTGGAAACTTGT+TGG	+	chr3.1:69103502-69103521	MS.gene75546:CDS	40.0%
	GATGAGAAGTCTTCATCATG+TGG	+	chr3.1:69103609-69103628	MS.gene75546:CDS	40.0%
	GTTTCTTCTTCCATGTCTCT+TGG	+	chr3.1:69103747-69103766	MS.gene75546:CDS	40.0%
	TGGTAGAAAGAATCACACTG+TGG	+	chr3.1:69103491-69103510	MS.gene75546:CDS	40.0%
!	CATGTGCAGTTTTTGAACCT+TGG	-	chr3.1:69103716-69103735	None:intergenic	40.0%
!	CCTCACCTTCAAAAGACTTT+TGG	+	chr3.1:69103543-69103562	MS.gene75546:CDS	40.0%
!	GCTGATTCCAGAAAGTACAA+GGG	+	chr3.1:69103399-69103418	MS.gene75546:CDS	40.0%
!!	AAAGTCTTTTGAAGGTGAGG+TGG	-	chr3.1:69103543-69103562	None:intergenic	40.0%
!!	CCAAAAGTCTTTTGAAGGTG+AGG	-	chr3.1:69103546-69103565	None:intergenic	40.0%
!!	GAAACTTGTTGGCAGAAACA+TGG	+	chr3.1:69103513-69103532	MS.gene75546:CDS	40.0%
!!	TGCTGATTCCAGAAAGTACA+AGG	+	chr3.1:69103398-69103417	MS.gene75546:CDS	40.0%
	AAGTACAAGGGAGACTCTTC+TGG	+	chr3.1:69103411-69103430	MS.gene75546:CDS	45.0%
	AGAGTCTCCCTTGTACTTTC+TGG	-	chr3.1:69103409-69103428	None:intergenic	45.0%
	GTACGTGTGTGTACTTTCTG+TGG	+	chr3.1:69103471-69103490	MS.gene75546:CDS	45.0%
	TGGTGAATGACCAAGAGACA+TGG	-	chr3.1:69103760-69103779	None:intergenic	45.0%
	TTGGTCATTCACCAACTGAC+AGG	+	chr3.1:69103766-69103785	MS.gene75546:CDS	45.0%
	CATTCACCAACTGACAGGCA+AGG	+	chr3.1:69103771-69103790	MS.gene75546:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	69103355	69103792	69103355	ID=MS.gene75546
chr3.1	mRNA	69103355	69103792	69103355	ID=MS.gene75546.t1;Parent=MS.gene75546
chr3.1	exon	69103355	69103792	69103355	ID=MS.gene75546.t1.exon1;Parent=MS.gene75546.t1
chr3.1	CDS	69103355	69103792	69103355	ID=cds.MS.gene75546.t1;Parent=MS.gene75546.t1

Gene Sequence

>MS.gene75546

GAAATTTTGCTTCACCTAGTGAAGATTCAGTTTTGATTAACTATGCTGATTCCAGAAAGTACAAGGGAGACTCTTCTGGAAAATCACAATCTCAAGGAACCAATTCTAAACATGGTGTACGTGTGTGTACTTTCTGTGGTAGAAAGAATCACACTGTGGAAACTTGTTGGCAGAAACATGGAGTTCCACCTCACCTTCAAAAGACTTTTGGTTCTTCATCTGCTAATCATATTGCAAAGGAGGATGTTACTAATGATGAGAAGTCTTCATCATGTGGTGATAGCAAAACTACTGCTCCAGCTATTACACATGAGCAATATGAGAAGTTAATATCTTTACTTCAGAATTCATCTCTTAACCAAGGTTCAAAAACTGCACATGCTTCTAATCAGGTTTCTTCTTCCATGTCTCTTGGTCATTCACCAACTGACAGGCAAG

Protein sequence

>MS.gene75546.t1

EILLHLVKIQFULTMLIPESTRETLLENHNLKEPILNMVYVCVLSVVERITLWKLVGRNMEFHLTFKRLLVLHLLIILQRRMLLMMRSLHHVVIAKLLLQLLHMSNMRSUYLYFRIHLLTKVQKLHMLLIRFLLPCLLVIHQLTGK

M. sativa cultivar XinJiangDaYe / MS.gene75546