AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75686

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75686.t1 MTR_3g084040 96.190 105 4 0 1 105 1 105 1.23e-72 211
MS.gene75686.t1 MTR_3g084040 95.960 99 4 0 1 99 1 99 5.17e-67 198
MS.gene75686.t1 MTR_4g109540 52.830 106 43 3 1 99 1 106 2.65e-29 106
MS.gene75686.t1 MTR_7g013730 46.847 111 49 2 1 101 1 111 1.14e-27 101
MS.gene75686.t1 MTR_7g013740 47.170 106 47 3 1 99 1 104 4.27e-27 97.4
MS.gene75686.t1 MTR_4g109540 49.123 114 43 4 1 99 1 114 1.51e-26 99.4
MS.gene75686.t1 MTR_3g025880 45.370 108 50 3 1 99 1 108 9.43e-24 94.0
MS.gene75686.t1 MTR_3g080130 54.412 68 30 1 36 102 36 103 3.80e-23 87.0
MS.gene75686.t1 MTR_7g105260 44.828 87 42 1 23 103 19 105 6.43e-21 81.3
MS.gene75686.t1 MTR_4g063560 42.045 88 50 1 15 101 12 99 1.01e-19 77.4
MS.gene75686.t1 MTR_4g070080 50.000 64 31 1 37 99 7 70 1.86e-19 77.4
MS.gene75686.t1 MTR_3g014000 43.617 94 47 2 1 94 1 88 2.57e-19 77.4
MS.gene75686.t1 MTR_4g070080 50.000 64 31 1 37 99 7 70 4.35e-19 77.4
MS.gene75686.t1 MTR_4g070190 48.438 64 32 1 37 99 9 72 5.89e-18 74.3
MS.gene75686.t1 MTR_7g099940 39.823 113 53 4 1 103 1 108 7.23e-18 73.6
MS.gene75686.t1 MTR_4g070140 48.485 66 33 1 37 101 9 74 2.64e-17 73.2
MS.gene75686.t1 MTR_1g092860 37.647 85 52 1 18 101 174 258 5.49e-16 71.2
MS.gene75686.t1 MTR_3g055990 57.407 54 23 0 37 90 7 60 4.85e-15 69.3
MS.gene75686.t1 MTR_3g055990 61.224 49 19 0 37 85 7 55 5.34e-15 69.3
MS.gene75686.t1 MTR_4g081520 44.928 69 37 1 34 101 49 117 6.21e-15 66.2
MS.gene75686.t1 MTR_3g075500 43.077 65 36 1 37 100 207 271 2.64e-14 66.6
MS.gene75686.t1 MTR_5g097580 58.000 50 21 0 35 84 5 54 5.83e-14 66.6
MS.gene75686.t1 MTR_5g097580 58.000 50 21 0 35 84 5 54 6.21e-14 66.2
MS.gene75686.t1 MTR_8g024520 42.424 66 38 0 36 101 25 90 8.13e-14 65.5
MS.gene75686.t1 MTR_8g024520 42.424 66 38 0 36 101 25 90 8.25e-14 65.5
MS.gene75686.t1 MTR_4g035310 44.828 58 32 0 36 93 16 73 1.02e-13 65.1
MS.gene75686.t1 MTR_4g035310 44.828 58 32 0 36 93 16 73 1.07e-13 65.1
MS.gene75686.t1 MTR_3g029580 36.047 86 51 3 18 100 13 97 1.13e-13 62.8
MS.gene75686.t1 MTR_3g075500 42.188 64 36 1 37 99 207 270 1.41e-13 64.7
MS.gene75686.t1 MTR_3g116130 39.394 66 40 0 36 101 29 94 1.47e-13 64.7
MS.gene75686.t1 MTR_3g116130 39.394 66 40 0 36 101 29 94 1.87e-13 64.7
MS.gene75686.t1 MTR_7g117495 43.103 58 33 0 36 93 18 75 1.96e-13 64.3
MS.gene75686.t1 MTR_6g004810 40.000 70 42 0 31 100 128 197 2.02e-13 64.7
MS.gene75686.t1 MTR_7g117495 43.103 58 33 0 36 93 18 75 2.07e-13 63.9
MS.gene75686.t1 MTR_3g029580 34.483 87 53 3 18 101 13 98 2.39e-13 61.6
MS.gene75686.t1 MTR_3g092490 46.552 58 31 0 36 93 26 83 2.51e-13 62.8
MS.gene75686.t1 MTR_5g064120 37.879 66 41 0 36 101 16 81 5.57e-13 63.2
MS.gene75686.t1 MTR_2g026180 43.478 69 38 1 33 101 1 68 7.36e-13 63.2
MS.gene75686.t1 MTR_4g104470 43.750 64 35 1 37 99 217 280 7.74e-13 62.8
MS.gene75686.t1 MTR_3g092490 46.552 58 31 0 36 93 26 83 7.78e-13 62.4
MS.gene75686.t1 MTR_3g092490 46.552 58 31 0 36 93 26 83 8.29e-13 62.4
MS.gene75686.t1 MTR_1g064230 45.312 64 34 1 36 98 196 259 1.21e-12 62.4
MS.gene75686.t1 MTR_2g096560 38.806 67 40 1 36 101 7 73 2.49e-12 58.2
MS.gene75686.t1 MTR_8g463890 42.667 75 38 1 33 102 70 144 3.01e-12 61.6
MS.gene75686.t1 MTR_6g477870 29.730 74 46 1 36 103 95 168 5.04e-12 59.3
MS.gene75686.t1 MTR_4g117350 39.706 68 40 1 34 100 69 136 5.98e-12 58.2
MS.gene75686.t1 MTR_1g045510 45.000 60 33 0 37 96 205 264 7.72e-12 60.1
MS.gene75686.t1 MTR_6g477870 31.818 66 44 1 36 100 95 160 8.47e-12 58.5
MS.gene75686.t1 MTR_4g081510 41.270 63 36 1 38 99 46 108 1.81e-11 57.8
MS.gene75686.t1 MTR_3g065190 43.939 66 35 2 36 99 112 177 1.85e-11 59.3
MS.gene75686.t1 MTR_4g074930 42.857 70 39 1 30 99 358 426 1.99e-11 59.3
MS.gene75686.t1 MTR_4g074930 42.857 70 39 1 30 99 370 438 1.99e-11 59.3
MS.gene75686.t1 MTR_5g017280 42.857 63 36 0 33 95 1 63 2.17e-11 58.9
MS.gene75686.t1 MTR_4g074930 42.857 70 39 1 30 99 323 391 2.20e-11 59.3
MS.gene75686.t1 MTR_4g074930 42.857 70 39 1 30 99 335 403 2.21e-11 59.3
MS.gene75686.t1 MTR_5g017280 42.857 63 36 0 33 95 1 63 2.28e-11 58.9
MS.gene75686.t1 MTR_4g124940 46.552 58 31 0 38 95 133 190 2.38e-11 58.9
MS.gene75686.t1 MTR_8g022050 48.276 58 30 0 38 95 149 206 3.01e-11 58.5
MS.gene75686.t1 MTR_7g106440 42.424 66 37 1 34 98 210 275 5.84e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75686.t1 AT3G23830 64.000 100 34 2 1 99 1 99 5.72e-41 132
MS.gene75686.t1 AT3G23830 64.000 100 34 2 1 99 1 99 5.72e-41 132
MS.gene75686.t1 AT4G13850 63.000 100 35 2 1 99 1 99 3.77e-39 127
MS.gene75686.t1 AT4G13850 63.000 100 35 2 1 99 1 99 5.11e-39 127
MS.gene75686.t1 AT4G13850 63.000 100 35 2 1 99 1 99 6.65e-39 127
MS.gene75686.t1 AT4G13850 63.000 100 35 2 1 99 1 99 7.70e-39 127
MS.gene75686.t1 AT5G61030 56.731 104 40 2 1 99 1 104 3.13e-32 114
MS.gene75686.t1 AT5G61030 56.731 104 40 2 1 99 1 104 3.13e-32 114
MS.gene75686.t1 AT5G47320 59.000 100 35 3 1 99 1 95 3.95e-32 112
MS.gene75686.t1 AT1G74230 54.545 99 43 1 1 99 1 97 1.04e-30 110
MS.gene75686.t1 AT3G08000 48.485 99 43 2 9 99 7 105 3.00e-22 85.1
MS.gene75686.t1 AT1G18630 38.889 108 55 3 1 101 4 107 2.16e-18 75.5
MS.gene75686.t1 AT2G37510 44.156 77 37 1 33 103 31 107 6.66e-18 73.9
MS.gene75686.t1 AT2G21660 48.438 64 32 1 37 99 9 72 1.37e-17 73.6
MS.gene75686.t1 AT2G21660 48.438 64 32 1 37 99 9 72 1.95e-17 73.6
MS.gene75686.t1 AT3G46020 47.059 68 35 1 35 101 6 73 1.31e-16 69.3
MS.gene75686.t1 AT5G06210 50.000 70 34 1 31 99 29 98 5.09e-16 68.9
MS.gene75686.t1 AT4G39260 36.986 73 40 1 37 103 7 79 1.09e-15 66.6
MS.gene75686.t1 AT2G37220 50.000 64 31 1 36 98 204 267 1.27e-15 70.5
MS.gene75686.t1 AT4G39260 36.986 73 40 1 37 103 7 79 1.90e-15 67.0
MS.gene75686.t1 AT3G53460 50.000 64 31 1 36 98 278 341 3.64e-15 69.7
MS.gene75686.t1 AT3G53460 50.000 64 31 1 36 98 257 320 3.68e-15 69.7
MS.gene75686.t1 AT3G53460 50.000 64 31 1 36 98 257 320 3.68e-15 69.7
MS.gene75686.t1 AT3G53460 50.000 64 31 1 36 98 257 320 3.68e-15 69.7
MS.gene75686.t1 AT4G39260 36.986 73 40 1 37 103 7 79 4.73e-15 67.0
MS.gene75686.t1 AT1G76460 46.552 58 31 0 36 93 24 81 7.02e-15 68.6
MS.gene75686.t1 AT4G39260 40.678 59 35 0 37 95 7 65 8.34e-15 64.7
MS.gene75686.t1 AT5G59865 47.826 69 35 1 34 101 24 92 8.88e-15 65.1
MS.gene75686.t1 AT3G20930 40.000 70 41 1 33 101 279 348 1.53e-14 68.2
MS.gene75686.t1 AT5G50250 45.312 64 34 1 37 99 208 271 1.79e-14 67.4
MS.gene75686.t1 AT3G13224 45.946 74 40 0 22 95 95 168 1.83e-14 66.6
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 1.87e-14 67.8
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 1.87e-14 67.8
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 1.87e-14 67.8
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 1.87e-14 67.8
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 1.99e-14 67.8
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 1.99e-14 67.8
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 1.99e-14 67.8
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 1.99e-14 67.8
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 1.99e-14 67.8
MS.gene75686.t1 AT5G47620 42.667 75 39 1 32 102 2 76 2.18e-14 67.8
MS.gene75686.t1 AT1G20880 46.552 58 31 0 36 93 24 81 2.27e-14 67.0
MS.gene75686.t1 AT1G20880 46.552 58 31 0 36 93 24 81 2.27e-14 67.0
MS.gene75686.t1 AT1G20880 46.552 58 31 0 36 93 24 81 2.27e-14 67.0
MS.gene75686.t1 AT1G22910 44.776 67 36 1 36 102 13 78 2.70e-14 66.2
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 2.73e-14 67.4
MS.gene75686.t1 AT5G55550 46.154 65 34 1 37 101 7 70 2.83e-14 67.4
MS.gene75686.t1 AT1G22910 44.776 67 36 1 36 102 13 78 2.98e-14 66.2
MS.gene75686.t1 AT3G07810 45.070 71 35 1 35 101 5 75 3.12e-14 67.4
MS.gene75686.t1 AT3G07810 45.070 71 35 1 35 101 5 75 3.21e-14 67.4
MS.gene75686.t1 AT3G13224 48.529 68 35 0 28 95 92 159 3.41e-14 67.0
MS.gene75686.t1 AT5G47620 45.205 73 36 1 33 101 3 75 4.56e-14 66.6
MS.gene75686.t1 AT5G47620 45.205 73 36 1 33 101 3 75 4.56e-14 66.6
MS.gene75686.t1 AT5G47620 45.205 73 36 1 33 101 3 75 4.56e-14 66.6
MS.gene75686.t1 AT4G24770 46.875 64 33 1 37 99 245 308 4.71e-14 66.6
MS.gene75686.t1 AT4G24770 46.875 64 33 1 37 99 245 308 4.71e-14 66.6
MS.gene75686.t1 AT3G13224 48.529 68 35 0 28 95 101 168 4.81e-14 66.6
MS.gene75686.t1 AT1G78260 39.394 66 40 0 36 101 17 82 5.23e-14 66.2
MS.gene75686.t1 AT1G78260 39.394 66 40 0 36 101 17 82 5.43e-14 65.9
MS.gene75686.t1 AT2G21690 46.429 56 30 0 39 94 10 65 7.35e-14 62.8
MS.gene75686.t1 AT1G33470 44.776 67 36 1 36 102 7 72 7.46e-14 65.1
MS.gene75686.t1 AT1G33470 44.776 67 36 1 36 102 7 72 7.46e-14 65.1
MS.gene75686.t1 AT1G33470 44.776 67 36 1 36 102 7 72 7.92e-14 65.1
MS.gene75686.t1 AT5G54580 44.928 69 37 1 34 101 54 122 8.48e-14 63.5
MS.gene75686.t1 AT3G26420 39.726 73 38 1 37 103 8 80 9.86e-14 64.7
MS.gene75686.t1 AT1G22910 46.552 58 31 0 36 93 13 70 1.21e-13 65.5
MS.gene75686.t1 AT2G46780 46.552 58 31 0 36 93 22 79 1.34e-13 65.1
MS.gene75686.t1 AT2G46780 46.552 58 31 0 36 93 22 79 1.34e-13 65.1
MS.gene75686.t1 AT2G22100 36.047 86 55 0 9 94 136 221 1.51e-13 65.1
MS.gene75686.t1 AT4G13860 46.970 66 34 1 35 99 2 67 1.63e-13 61.2
MS.gene75686.t1 AT1G60000 50.000 70 34 1 30 98 171 240 1.67e-13 64.3
MS.gene75686.t1 AT1G22330 39.394 66 40 0 36 101 17 82 3.67e-13 63.9
MS.gene75686.t1 AT1G60650 42.623 61 35 0 35 95 11 71 4.89e-13 63.5
MS.gene75686.t1 AT1G60650 42.623 61 35 0 35 95 11 71 4.89e-13 63.5
MS.gene75686.t1 AT4G26650 44.615 65 35 1 37 101 16 79 6.42e-13 63.5
MS.gene75686.t1 AT4G26650 44.615 65 35 1 37 101 13 76 6.67e-13 63.5
MS.gene75686.t1 AT1G17640 43.939 66 36 1 35 100 156 220 6.84e-13 63.2
MS.gene75686.t1 AT1G17640 42.623 61 35 0 37 97 67 127 7.53e-11 57.4
MS.gene75686.t1 AT1G17640 43.939 66 36 1 35 100 156 220 6.84e-13 63.2
MS.gene75686.t1 AT1G17640 42.623 61 35 0 37 97 67 127 7.53e-11 57.4
MS.gene75686.t1 AT1G17640 43.939 66 36 1 35 100 156 220 6.84e-13 63.2
MS.gene75686.t1 AT1G17640 42.623 61 35 0 37 97 67 127 7.53e-11 57.4
MS.gene75686.t1 AT3G52380 48.485 66 33 1 34 98 217 282 7.06e-13 63.2
MS.gene75686.t1 AT2G27330 41.333 75 38 1 35 103 20 94 7.79e-13 60.1
MS.gene75686.t1 AT1G01080 33.333 90 60 0 11 100 187 276 1.05e-12 62.4
MS.gene75686.t1 AT5G11412 36.364 66 42 0 36 101 52 117 1.33e-12 60.8
MS.gene75686.t1 AT4G14300 55.102 49 22 0 37 85 7 55 1.83e-12 62.0
MS.gene75686.t1 AT4G14300 55.102 49 22 0 37 85 7 55 1.83e-12 62.0
MS.gene75686.t1 AT3G20930 37.333 75 41 2 33 101 279 353 2.69e-12 61.6
MS.gene75686.t1 AT2G37510 33.333 102 37 2 33 103 31 132 2.83e-12 59.7
MS.gene75686.t1 AT1G54080 44.615 65 35 1 38 101 150 214 3.29e-12 61.6
MS.gene75686.t1 AT2G19380 32.558 86 58 0 9 94 381 466 3.34e-12 61.6
MS.gene75686.t1 AT1G01080 32.955 88 59 0 11 98 187 274 3.97e-12 60.8
MS.gene75686.t1 AT1G01080 32.955 88 59 0 11 98 188 275 4.07e-12 60.8
MS.gene75686.t1 AT5G40490 39.394 66 40 0 35 100 129 194 4.23e-12 61.2
MS.gene75686.t1 AT5G40490 39.394 66 40 0 35 100 112 177 5.29e-12 60.8
MS.gene75686.t1 AT3G54770 41.379 58 34 0 36 93 17 74 6.46e-12 60.1
MS.gene75686.t1 AT3G54770 41.379 58 34 0 36 93 17 74 6.99e-12 60.1
MS.gene75686.t1 AT5G53680 41.379 58 34 0 36 93 13 70 1.04e-11 58.2
MS.gene75686.t1 AT5G04280 40.678 59 35 0 36 94 7 65 1.48e-11 59.3
MS.gene75686.t1 AT4G36960 44.828 58 32 0 37 94 4 61 2.55e-11 58.9
MS.gene75686.t1 AT4G36960 44.828 58 32 0 37 94 4 61 2.55e-11 58.9
MS.gene75686.t1 AT3G15010 33.010 103 64 1 5 102 44 146 4.02e-11 58.5
MS.gene75686.t1 AT3G15010 33.010 103 64 1 5 102 44 146 4.02e-11 58.5
MS.gene75686.t1 AT3G14100 41.538 65 37 1 38 101 146 210 4.70e-11 58.2
MS.gene75686.t1 AT2G33410 48.980 49 25 0 37 85 7 55 6.60e-11 57.8

Find 26 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTGGAAGATCAAGGGGATT+TGG 0.334462 3.3:+70497239 MS.gene75686:CDS
ATGGCTTTCTGTAACAAGAT+TGG 0.370753 3.3:+70496545 MS.gene75686:CDS
TGTTGATGACCAGTCTCTTA+AGG 0.428494 3.3:+70496775 MS.gene75686:CDS
TAGTTAAGCATGGATGAAAC+AGG 0.461015 3.3:-70496608 None:intergenic
AGCACTCTCCATGGATGGAC+AGG 0.490812 3.3:+70497298 MS.gene75686:CDS
ATAGAGACACTGGAAGATCA+AGG 0.517492 3.3:+70497231 MS.gene75686:CDS
TAAATGCATCCTTAAGAGAC+TGG 0.532048 3.3:-70496784 None:intergenic
AAGGATGCATTTACAACCTA+CGG 0.532917 3.3:+70496794 MS.gene75686:CDS
GGTCATCAACATGGTAAGAA+AGG 0.538171 3.3:-70496763 None:intergenic
TATTACAACCTGTCCATCCA+TGG 0.550532 3.3:-70497306 None:intergenic
TGAAGTTGCAGACTCTTCAC+TGG 0.556568 3.3:-70497276 None:intergenic
TGCAACTTCAGCACTCTCCA+TGG 0.559718 3.3:+70497289 MS.gene75686:CDS
AACCTACGGAGAAGTGGTTG+AGG 0.563954 3.3:+70496808 MS.gene75686:CDS
ACTTCAGCACTCTCCATGGA+TGG 0.565520 3.3:+70497293 MS.gene75686:CDS
GAGTAGCACCTTGCCTCAAG+AGG 0.579009 3.3:-70496571 None:intergenic
GTTATCACTGATAGAGACAC+TGG 0.581409 3.3:+70497221 MS.gene75686:CDS
TAGAGACACTGGAAGATCAA+GGG 0.594765 3.3:+70497232 MS.gene75686:CDS
ACCTACGGAGAAGTGGTTGA+GGG 0.603405 3.3:+70496809 MS.gene75686:CDS
AGAGACACTGGAAGATCAAG+GGG 0.604078 3.3:+70497233 MS.gene75686:CDS
ATTTACAACCTACGGAGAAG+TGG 0.607456 3.3:+70496802 MS.gene75686:CDS
GTGGCGCAAGTAGTTAAGCA+TGG 0.622132 3.3:-70496618 None:intergenic
ATTGGAAACCTCTTGAGGCA+AGG 0.631105 3.3:+70496563 MS.gene75686:CDS
TAAGAGACTGGTCATCAACA+TGG 0.631495 3.3:-70496772 None:intergenic
ACAAGATTGGAAACCTCTTG+AGG 0.658250 3.3:+70496558 MS.gene75686:CDS
ACCCTCAACCACTTCTCCGT+AGG 0.658961 3.3:-70496810 None:intergenic
AAAGCTTGCTTGAAGACATG+TGG 0.718559 3.3:-70496637 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTATAATAAATAACTT+TGG + chr3.3:70497179-70497198 MS.gene75686:intron 10.0%
!! GAATGTTAGAAGTTAAATTT+GGG + chr3.3:70496929-70496948 MS.gene75686:intron 20.0%
!!! TCTAATAAAATGTATGGTTT+TGG + chr3.3:70496724-70496743 MS.gene75686:intron 20.0%
!!! TTTATCTAGTTTTTTTATGC+TGG + chr3.3:70496682-70496701 MS.gene75686:intron 20.0%
! AGTGTTATGTTGATACAAAA+GGG - chr3.3:70496897-70496916 None:intergenic 25.0%
! GCTGGTTAGTATTATTATTA+TGG + chr3.3:70496700-70496719 MS.gene75686:intron 25.0%
! GGAATGTTAGAAGTTAAATT+TGG + chr3.3:70496928-70496947 MS.gene75686:intron 25.0%
!! TTGATACATTAAAGTGTCTT+TGG + chr3.3:70497102-70497121 MS.gene75686:intron 25.0%
!!! AAATGTATGGTTTTGGATTT+TGG + chr3.3:70496731-70496750 MS.gene75686:intron 25.0%
!!! TATGGCTCTAATAAAATGTA+TGG + chr3.3:70496718-70496737 MS.gene75686:intron 25.0%
CACTTTAATGTATCAACTAC+CGG - chr3.3:70497098-70497117 None:intergenic 30.0%
CAGTGTTATGTTGATACAAA+AGG - chr3.3:70496898-70496917 None:intergenic 30.0%
GTGTTATGTTGATACAAAAG+GGG - chr3.3:70496896-70496915 None:intergenic 30.0%
! TGTTGTAATTCTGAAGAGTA+AGG + chr3.3:70497065-70497084 MS.gene75686:intron 30.0%
!! GTTTTGGATTTTGGTATCAT+AGG + chr3.3:70496740-70496759 MS.gene75686:intron 30.0%
!! TTTCATTGGAGGTATTGATT+TGG + chr3.3:70496658-70496677 MS.gene75686:intron 30.0%
AAGGATGCATTTACAACCTA+CGG + chr3.3:70496794-70496813 MS.gene75686:CDS 35.0%
ATGGCTTTCTGTAACAAGAT+TGG + chr3.3:70496545-70496564 MS.gene75686:CDS 35.0%
TAAATGCATCCTTAAGAGAC+TGG - chr3.3:70496787-70496806 None:intergenic 35.0%
TAGTTAAGCATGGATGAAAC+AGG - chr3.3:70496611-70496630 None:intergenic 35.0%
TGAAGAGTAAGGCAAATTTC+CGG + chr3.3:70497076-70497095 MS.gene75686:intron 35.0%
! ACATAACACTGCATTGACAT+TGG + chr3.3:70496907-70496926 MS.gene75686:intron 35.0%
! TCTTCAAGCAAGCTTTTCAT+TGG + chr3.3:70496644-70496663 MS.gene75686:CDS 35.0%
!! GCATCTGCTTTGTTTGATTA+TGG + chr3.3:70497155-70497174 MS.gene75686:intron 35.0%
ACAAGATTGGAAACCTCTTG+AGG + chr3.3:70496558-70496577 MS.gene75686:CDS 40.0%
ATAGAGACACTGGAAGATCA+AGG + chr3.3:70497231-70497250 MS.gene75686:CDS 40.0%
ATTTACAACCTACGGAGAAG+TGG + chr3.3:70496802-70496821 MS.gene75686:CDS 40.0%
CTGAAGAACACATTTCAGTG+GGG + chr3.3:70496968-70496987 MS.gene75686:intron 40.0%
GCTGAAGAACACATTTCAGT+GGG + chr3.3:70496967-70496986 MS.gene75686:intron 40.0%
GGTCATCAACATGGTAAGAA+AGG - chr3.3:70496766-70496785 None:intergenic 40.0%
GTTATCACTGATAGAGACAC+TGG + chr3.3:70497221-70497240 MS.gene75686:CDS 40.0%
TAGAGACACTGGAAGATCAA+GGG + chr3.3:70497232-70497251 MS.gene75686:CDS 40.0%
TATTACAACCTGTCCATCCA+TGG - chr3.3:70497309-70497328 None:intergenic 40.0%
TGCTGAAGAACACATTTCAG+TGG + chr3.3:70496966-70496985 MS.gene75686:intron 40.0%
! AAAGCTTGCTTGAAGACATG+TGG - chr3.3:70496640-70496659 None:intergenic 40.0%
! TAAGAGACTGGTCATCAACA+TGG - chr3.3:70496775-70496794 None:intergenic 40.0%
! TCAAGCAAGCTTTTCATTGG+AGG + chr3.3:70496647-70496666 MS.gene75686:CDS 40.0%
!! TGTTGATGACCAGTCTCTTA+AGG + chr3.3:70496775-70496794 MS.gene75686:CDS 40.0%
ACTGGAAGATCAAGGGGATT+TGG + chr3.3:70497239-70497258 MS.gene75686:CDS 45.0%
AGAGACACTGGAAGATCAAG+GGG + chr3.3:70497233-70497252 MS.gene75686:CDS 45.0%
ATTGGAAACCTCTTGAGGCA+AGG + chr3.3:70496563-70496582 MS.gene75686:CDS 45.0%
TGAAGTTGCAGACTCTTCAC+TGG - chr3.3:70497279-70497298 None:intergenic 45.0%
AACCTACGGAGAAGTGGTTG+AGG + chr3.3:70496808-70496827 MS.gene75686:CDS 50.0%
ACCTACGGAGAAGTGGTTGA+GGG + chr3.3:70496809-70496828 MS.gene75686:CDS 50.0%
ACTTCAGCACTCTCCATGGA+TGG + chr3.3:70497293-70497312 MS.gene75686:CDS 50.0%
GTGGCGCAAGTAGTTAAGCA+TGG - chr3.3:70496621-70496640 None:intergenic 50.0%
TGCAACTTCAGCACTCTCCA+TGG + chr3.3:70497289-70497308 MS.gene75686:CDS 50.0%
ACCCTCAACCACTTCTCCGT+AGG - chr3.3:70496813-70496832 None:intergenic 55.0%
AGCACTCTCCATGGATGGAC+AGG + chr3.3:70497298-70497317 MS.gene75686:CDS 55.0%
GAGTAGCACCTTGCCTCAAG+AGG - chr3.3:70496574-70496593 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 70496545 70497340 70496545 ID=MS.gene75686
chr3.3 mRNA 70496545 70497340 70496545 ID=MS.gene75686.t1;Parent=MS.gene75686
chr3.3 exon 70496545 70496668 70496545 ID=MS.gene75686.t1.exon1;Parent=MS.gene75686.t1
chr3.3 CDS 70496545 70496668 70496545 ID=cds.MS.gene75686.t1;Parent=MS.gene75686.t1
chr3.3 exon 70496762 70496830 70496762 ID=MS.gene75686.t1.exon2;Parent=MS.gene75686.t1
chr3.3 CDS 70496762 70496830 70496762 ID=cds.MS.gene75686.t1;Parent=MS.gene75686.t1
chr3.3 exon 70497216 70497340 70497216 ID=MS.gene75686.t1.exon3;Parent=MS.gene75686.t1
chr3.3 CDS 70497216 70497340 70497216 ID=cds.MS.gene75686.t1;Parent=MS.gene75686.t1
Gene Sequence

>MS.gene75686

ATGGCTTTCTGTAACAAGATTGGAAACCTCTTGAGGCAAGGTGCTACTCAGAGCACTCAAGCTCCTGTTTCATCCATGCTTAACTACTTGCGCCACATGTCTTCAAGCAAGCTTTTCATTGGAGGTATTGATTTGGTTTTATCTAGTTTTTTTATGCTGGTTAGTATTATTATTATGGCTCTAATAAAATGTATGGTTTTGGATTTTGGTATCATAGGCCTTTCTTACCATGTTGATGACCAGTCTCTTAAGGATGCATTTACAACCTACGGAGAAGTGGTTGAGGGTGAGTTCATCTTAATTTATTGTTAAGTGAATAAATTTAGCTTCATTTTGCAATGTGTTTATCCCCTTTTGTATCAACATAACACTGCATTGACATTGGAATGTTAGAAGTTAAATTTGGGTAATTTGTATTGAATGCTGAAGAACACATTTCAGTGGGGAATATGTTTTGTCTTTGAATTTTGAGTAGACATGCTATTTACAATCTTAGCAAGCAAACATGATATCTGTAACTTGTTGTAATTCTGAAGAGTAAGGCAAATTTCCGGTAGTTGATACATTAAAGTGTCTTTGGTTTATGATTATTGAAACAAAAAAAAAAAAAGCATCTGCTTTGTTTGATTATGGTGTTTTTATAATAAATAACTTTGGCTGTGTTTTTGCAGCAAGAGTTATCACTGATAGAGACACTGGAAGATCAAGGGGATTTGGATTTGTCAACTTCACCAGTGAAGAGTCTGCAACTTCAGCACTCTCCATGGATGGACAGGTTGTAATATTATGCTGTTAA
Protein sequence

>MS.gene75686.t1

MAFCNKIGNLLRQGATQSTQAPVSSMLNYLRHMSSSKLFIGGLSYHVDDQSLKDAFTTYGEVVEARVITDRDTGRSRGFGFVNFTSEESATSALSMDGQVVILCC