AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene75801

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75801.t1 MTR_5g099180 95.538 381 11 4 3 381 2 378 0.0 726
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene75801.t1 AT2G17265 70.699 372 98 4 9 378 6 368 5.59e-164 464
MS.gene75801.t1 AT4G35295 57.143 63 22 1 316 378 54 111 3.17e-13 66.2

Find 112 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGTTGGGGCTTCAAATTC+TGG 0.168994 5.3:-80469973 None:intergenic
AAATCAGAAGAGAAGGTTTC+TGG 0.179307 5.3:+80469823 MS.gene75801:CDS
TCTTGTGGCGTCAGTGTTAC+AGG 0.194081 5.3:+80470068 MS.gene75801:CDS
GCCATTCAAGCTGGAGCTTT+TGG 0.204037 5.3:+80470189 MS.gene75801:CDS
TTACCTTTGGGAAGTGGTTT+GGG 0.235266 5.3:+80469706 MS.gene75801:CDS
TTTACCTTTGGGAAGTGGTT+TGG 0.257806 5.3:+80469705 MS.gene75801:CDS
ATATCCATCTCACATATTTC+TGG 0.261329 5.3:+80469556 MS.gene75801:CDS
ATCTCACCGGGGTGAACATC+TGG 0.273214 5.3:-80469535 None:intergenic
CCTGCCACCGTCGCCAATCT+AGG 0.297972 5.3:+80469445 MS.gene75801:CDS
TGCAGCGGCGCTGGCAGCAC+TGG 0.313196 5.3:-80469731 None:intergenic
TCTCGAAAAGGGTTTACCTT+TGG 0.330871 5.3:+80469693 MS.gene75801:CDS
TTACTGAGTTTGTGGATGTT+AGG 0.331672 5.3:-80469586 None:intergenic
CTCGAAAAGGGTTTACCTTT+GGG 0.332798 5.3:+80469694 MS.gene75801:CDS
CTTAGGTTCAACAATCTTAT+CGG 0.352515 5.3:-80470124 None:intergenic
GCAGCACTGCCTTTAGAGAT+AGG 0.366224 5.3:+80470006 MS.gene75801:CDS
TCTTTCACTTTCTCTCGAAA+AGG 0.371475 5.3:+80469681 MS.gene75801:CDS
TAGGGATGCCGCACCATGTC+TGG 0.375083 5.3:+80470025 MS.gene75801:CDS
AAGCGTGCACCATTGATTCC+TGG 0.387995 5.3:+80470144 MS.gene75801:CDS
GATTCGCAATTACCAACCTT+TGG 0.392630 5.3:+80469894 MS.gene75801:CDS
TGCCAGCGCCGCTGCAGCCG+CGG 0.400058 5.3:+80469738 MS.gene75801:CDS
AACCGCGGCTGCAGCGGCGC+TGG 0.405837 5.3:-80469740 None:intergenic
GGCTGCACCATCAGTGGTGC+TGG 0.412382 5.3:+80470210 MS.gene75801:CDS
CCTAGATTGGCGACGGTGGC+AGG 0.418200 5.3:-80469445 None:intergenic
ATCGAAGCCGGGACCTAGAT+TGG 0.418554 5.3:-80469458 None:intergenic
TTGCTGTCAATGAGATCTTC+GGG 0.419028 5.3:+80469761 MS.gene75801:CDS
CTTTCACTTTCTCTCGAAAA+GGG 0.421777 5.3:+80469682 MS.gene75801:CDS
GTTGCTGTCAATGAGATCTT+CGG 0.437235 5.3:+80469760 MS.gene75801:CDS
ACGCCACAAGAGCAGCTGCC+TGG 0.444695 5.3:-80470056 None:intergenic
GGAATCAATGGTGCACGCTT+AGG 0.462471 5.3:-80470141 None:intergenic
TGTCACTGATAACGAGGAAA+AGG 0.462571 5.3:+80470248 MS.gene75801:CDS
GCCAAAAGCTCCAGCTTGAA+TGG 0.464692 5.3:-80470190 None:intergenic
GGTCCCGGCTTCGATTTCCT+TGG 0.466367 5.3:+80469466 MS.gene75801:CDS
GGCAGGAGCAAAGGCCTTGA+CGG 0.467231 5.3:-80469428 None:intergenic
GCAGCCAAGGAAATCGAAGC+CGG 0.468475 5.3:-80469470 None:intergenic
TTCCTTGGCTGCGCTGTCGA+TGG 0.471936 5.3:+80469481 MS.gene75801:CDS
GTCACTGATAACGAGGAAAA+GGG 0.476723 5.3:+80470249 MS.gene75801:CDS
AAGGGTTTACCTTTGGGAAG+TGG 0.480325 5.3:+80469700 MS.gene75801:CDS
CAATGTTGCCCCATCCATCA+TGG 0.480430 5.3:+80469858 MS.gene75801:CDS
AACATCTGGGTCCACTTTGA+CGG 0.481780 5.3:-80469521 None:intergenic
CTTGTGGCGTCAGTGTTACA+GGG 0.482574 5.3:+80470069 MS.gene75801:CDS
GACAGCAACCGCGGCTGCAG+CGG 0.482714 5.3:-80469746 None:intergenic
CATGTTGTTGAAGCAGCCAT+TGG 0.487615 5.3:-80469247 None:intergenic
CTTCTCTTCTGATTTCAAGC+AGG 0.492990 5.3:-80469815 None:intergenic
CAACAATCTTATCGGAGGAC+AGG 0.494191 5.3:-80470116 None:intergenic
AAGAAGGCTGCCATTCAAGC+TGG 0.496033 5.3:+80470180 MS.gene75801:CDS
TGTTTGAAATTAAAGGAAAG+TGG 0.496328 5.3:-80469292 None:intergenic
CCATGTCTGGAATTCTAGCC+AGG 0.497827 5.3:+80470038 MS.gene75801:CDS
AGCGTGCACCATTGATTCCT+GGG 0.501591 5.3:+80470145 MS.gene75801:CDS
GCATCCATCCCAGGAATCAA+TGG 0.507028 5.3:-80470153 None:intergenic
TGCCATCGACAGCGCAGCCA+AGG 0.508599 5.3:-80469483 None:intergenic
ACCTGTTTATTCTTCCGTCA+AGG 0.511408 5.3:+80469414 MS.gene75801:CDS
GTCAAGCAGCTTGATCGCCT+TGG 0.519083 5.3:+80470339 MS.gene75801:CDS
GGCTGCGCTGTCGATGGCAT+CGG 0.519529 5.3:+80469487 MS.gene75801:CDS
ACGGAAGAATAAACAGGTGG+TGG 0.522057 5.3:-80469409 None:intergenic
ACCGTCGCCAATCTAGGTCC+CGG 0.523818 5.3:+80469451 MS.gene75801:CDS
ACGGCATCAATTCCAAAGGT+TGG 0.527087 5.3:-80469906 None:intergenic
AATGTTGCCCCATCCATCAT+GGG 0.527769 5.3:+80469859 MS.gene75801:CDS
GGCGACGGTGGCAGGAGCAA+AGG 0.532400 5.3:-80469437 None:intergenic
TAGCCAGGCAGCTGCTCTTG+TGG 0.536109 5.3:+80470053 MS.gene75801:CDS
AACCCTCTTTGGAACTGCGC+TGG 0.536246 5.3:+80469610 MS.gene75801:CDS
CAGCACTGCCTTTAGAGATA+GGG 0.537569 5.3:+80470007 MS.gene75801:CDS
GCGATCAAGCTGCTTGACAT+TGG 0.539004 5.3:-80470334 None:intergenic
CAGCCAAGGAAATCGAAGCC+GGG 0.542736 5.3:-80469469 None:intergenic
TTCTTGACAGCATCCATCCC+AGG 0.549795 5.3:-80470162 None:intergenic
TGCGGCATCCCTATCTCTAA+AGG 0.558659 5.3:-80470015 None:intergenic
TGGGATGGATGCTGTCAAGA+AGG 0.561159 5.3:+80470164 MS.gene75801:CDS
GCCTTGACGGAAGAATAAAC+AGG 0.565015 5.3:-80469415 None:intergenic
TCTCACCGGGGTGAACATCT+GGG 0.567871 5.3:-80469534 None:intergenic
TGCACCATTGATTCCTGGGA+TGG 0.572503 5.3:+80470149 MS.gene75801:CDS
GGATTTGTTCATGTTAGGGA+AGG 0.572617 5.3:+80470092 MS.gene75801:CDS
CTGCTTGAAATCAGAAGAGA+AGG 0.573937 5.3:+80469816 MS.gene75801:CDS
ATGTGAGATGGATATCTCAC+CGG 0.575914 5.3:-80469548 None:intergenic
AGTAGGACCAGCACCACTGA+TGG 0.581134 5.3:-80470217 None:intergenic
TGTGAGATGGATATCTCACC+GGG 0.582722 5.3:-80469547 None:intergenic
TACCAGCGCAGTTCCAAAGA+GGG 0.584554 5.3:-80469612 None:intergenic
ATTTCAAGCAGGCGAGAACA+AGG 0.584734 5.3:-80469804 None:intergenic
TCGGTAAGTGTGACGACGGA+GGG 0.586137 5.3:-80469385 None:intergenic
AGAATAAACAGGTGGTGGGT+CGG 0.588466 5.3:-80469404 None:intergenic
AACAATCTTATCGGAGGACA+GGG 0.598923 5.3:-80470115 None:intergenic
TTCAACGGCATCAATTCCAA+AGG 0.604897 5.3:-80469910 None:intergenic
TCAGTGACAGCAACCGCAGT+AGG 0.607546 5.3:-80470234 None:intergenic
ATGCTCCGCATTCGATCCGT+CGG 0.611634 5.3:+80469658 MS.gene75801:CDS
GCCGGGACCTAGATTGGCGA+CGG 0.611775 5.3:-80469452 None:intergenic
TTGACCAGAAATATGTGAGA+TGG 0.612753 5.3:-80469560 None:intergenic
ATGTTGCCCCATCCATCATG+GGG 0.617492 5.3:+80469860 MS.gene75801:CDS
GTTGCCCCATCCATCATGGG+GGG 0.619596 5.3:+80469862 MS.gene75801:CDS
CTTCGGGAAAAGATTGAGTG+TGG 0.620572 5.3:+80469777 MS.gene75801:CDS
CCTGGCTAGAATTCCAGACA+TGG 0.621126 5.3:-80470038 None:intergenic
AAAGACCGACGGATCGAATG+CGG 0.626540 5.3:-80469663 None:intergenic
TGACACTGTCTCCGTCAAAG+TGG 0.628597 5.3:+80469510 MS.gene75801:CDS
GCACATCATTGCTCAACACA+TGG 0.632675 5.3:+80470272 MS.gene75801:CDS
CGGAAGAATAAACAGGTGGT+GGG 0.638411 5.3:-80469408 None:intergenic
AGGTTCAACAATCTTATCGG+AGG 0.639350 5.3:-80470121 None:intergenic
CTAGAATTCCAGACATGGTG+CGG 0.639933 5.3:-80470033 None:intergenic
GATCTCATTGACAGCAACCG+CGG 0.645517 5.3:-80469755 None:intergenic
TCACTGATAACGAGGAAAAG+GGG 0.646942 5.3:+80470250 MS.gene75801:CDS
GGAGGGATGCTTCTTCTGAG+AGG 0.647790 5.3:-80469368 None:intergenic
TTGTGGCGTCAGTGTTACAG+GGG 0.658642 5.3:+80470070 MS.gene75801:CDS
GGGACCTAGATTGGCGACGG+TGG 0.667548 5.3:-80469449 None:intergenic
GATCCCAAACCACTTCCCAA+AGG 0.668028 5.3:-80469709 None:intergenic
GTCGGTAAGTGTGACGACGG+AGG 0.671687 5.3:-80469386 None:intergenic
GTGGACCCAGATGTTCACCC+CGG 0.674789 5.3:+80469529 MS.gene75801:CDS
ATACCAGCGCAGTTCCAAAG+AGG 0.675755 5.3:-80469613 None:intergenic
TGTGGCGTCAGTGTTACAGG+GGG 0.680350 5.3:+80470071 MS.gene75801:CDS
GAGAGAAAGTGAAAGACCGA+CGG 0.682882 5.3:-80469674 None:intergenic
TGCTAATAAGTCTAGCACCA+AGG 0.687849 5.3:-80470356 None:intergenic
CAGTGGTGCTGGTCCTACTG+CGG 0.694225 5.3:+80470221 MS.gene75801:CDS
TGTTGCCCCATCCATCATGG+GGG 0.701496 5.3:+80469861 MS.gene75801:CDS
TGGGTCGGTAAGTGTGACGA+CGG 0.704433 5.3:-80469389 None:intergenic
GGTTGCTGTCACTGATAACG+AGG 0.710300 5.3:+80470242 MS.gene75801:CDS
TTGACGGAAGAATAAACAGG+TGG 0.720068 5.3:-80469412 None:intergenic
GTGAGATGGATATCTCACCG+GGG 0.829626 5.3:-80469546 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCTTTTGTTTGAAATTAA+AGG - chr5.3:80469302-80469321 None:intergenic 15.0%
!! TGTTTGAAATTAAAGGAAAG+TGG - chr5.3:80469295-80469314 None:intergenic 25.0%
!!! AAATATAGCTGTTTTTCAGA+TGG - chr5.3:80469940-80469959 None:intergenic 25.0%
!!! AATATAGCTGTTTTTCAGAT+GGG - chr5.3:80469939-80469958 None:intergenic 25.0%
ATATCCATCTCACATATTTC+TGG + chr5.3:80469556-80469575 MS.gene75801:CDS 30.0%
CTTAGGTTCAACAATCTTAT+CGG - chr5.3:80470127-80470146 None:intergenic 30.0%
!!! CTGCTCTCATTTTTTTAGTT+GGG - chr5.3:80469990-80470009 None:intergenic 30.0%
!!! TGCTCTCATTTTTTTAGTTG+GGG - chr5.3:80469989-80470008 None:intergenic 30.0%
AAATCAGAAGAGAAGGTTTC+TGG + chr5.3:80469823-80469842 MS.gene75801:CDS 35.0%
AACTCAGTAAAAACCCTCTT+TGG + chr5.3:80469599-80469618 MS.gene75801:CDS 35.0%
CTTTCACTTTCTCTCGAAAA+GGG + chr5.3:80469682-80469701 MS.gene75801:CDS 35.0%
TCTTTCACTTTCTCTCGAAA+AGG + chr5.3:80469681-80469700 MS.gene75801:CDS 35.0%
TTACTGAGTTTGTGGATGTT+AGG - chr5.3:80469589-80469608 None:intergenic 35.0%
TTGACCAGAAATATGTGAGA+TGG - chr5.3:80469563-80469582 None:intergenic 35.0%
! CATGGTTCAAGCTTTTCAAA+AGG + chr5.3:80470290-80470309 MS.gene75801:CDS 35.0%
! GTTCAAGCTTTTCAAAAGGA+AGG + chr5.3:80470294-80470313 MS.gene75801:CDS 35.0%
! TTTTCAGATGGGAACTTCAA+CGG - chr5.3:80469928-80469947 None:intergenic 35.0%
!!! GCTGCTCTCATTTTTTTAGT+TGG - chr5.3:80469991-80470010 None:intergenic 35.0%
AACAATCTTATCGGAGGACA+GGG - chr5.3:80470118-80470137 None:intergenic 40.0%
ACCTGTTTATTCTTCCGTCA+AGG + chr5.3:80469414-80469433 MS.gene75801:CDS 40.0%
AGGTTCAACAATCTTATCGG+AGG - chr5.3:80470124-80470143 None:intergenic 40.0%
ATGTGAGATGGATATCTCAC+CGG - chr5.3:80469551-80469570 None:intergenic 40.0%
CTCGAAAAGGGTTTACCTTT+GGG + chr5.3:80469694-80469713 MS.gene75801:CDS 40.0%
CTGCTTGAAATCAGAAGAGA+AGG + chr5.3:80469816-80469835 MS.gene75801:CDS 40.0%
CTTCTCTTCTGATTTCAAGC+AGG - chr5.3:80469818-80469837 None:intergenic 40.0%
GATTCGCAATTACCAACCTT+TGG + chr5.3:80469894-80469913 MS.gene75801:CDS 40.0%
GTTGCTGTCAATGAGATCTT+CGG + chr5.3:80469760-80469779 MS.gene75801:CDS 40.0%
TCTCGAAAAGGGTTTACCTT+TGG + chr5.3:80469693-80469712 MS.gene75801:CDS 40.0%
TGCTAATAAGTCTAGCACCA+AGG - chr5.3:80470359-80470378 None:intergenic 40.0%
TTAGTTGGGGCTTCAAATTC+TGG - chr5.3:80469976-80469995 None:intergenic 40.0%
TTCAACGGCATCAATTCCAA+AGG - chr5.3:80469913-80469932 None:intergenic 40.0%
TTGACGGAAGAATAAACAGG+TGG - chr5.3:80469415-80469434 None:intergenic 40.0%
TTGCTGTCAATGAGATCTTC+GGG + chr5.3:80469761-80469780 MS.gene75801:CDS 40.0%
! AGGGGGATTTGTTCATGTTA+GGG + chr5.3:80470088-80470107 MS.gene75801:CDS 40.0%
! CATTTTCATGGCTTCGATAG+CGG - chr5.3:80469641-80469660 None:intergenic 40.0%
! GAGGGTTTTTACTGAGTTTG+TGG - chr5.3:80469597-80469616 None:intergenic 40.0%
! GGATTTGTTCATGTTAGGGA+AGG + chr5.3:80470092-80470111 MS.gene75801:CDS 40.0%
! GTCACTGATAACGAGGAAAA+GGG + chr5.3:80470249-80470268 MS.gene75801:CDS 40.0%
! TCACTGATAACGAGGAAAAG+GGG + chr5.3:80470250-80470269 MS.gene75801:CDS 40.0%
! TGTCACTGATAACGAGGAAA+AGG + chr5.3:80470248-80470267 MS.gene75801:CDS 40.0%
! TTACCTTTGGGAAGTGGTTT+GGG + chr5.3:80469706-80469725 MS.gene75801:CDS 40.0%
! TTTACCTTTGGGAAGTGGTT+TGG + chr5.3:80469705-80469724 MS.gene75801:CDS 40.0%
AACATCTGGGTCCACTTTGA+CGG - chr5.3:80469524-80469543 None:intergenic 45.0%
AATGTTGCCCCATCCATCAT+GGG + chr5.3:80469859-80469878 MS.gene75801:CDS 45.0%
ACGGAAGAATAAACAGGTGG+TGG - chr5.3:80469412-80469431 None:intergenic 45.0%
ACGGCATCAATTCCAAAGGT+TGG - chr5.3:80469909-80469928 None:intergenic 45.0%
ATTTCAAGCAGGCGAGAACA+AGG - chr5.3:80469807-80469826 None:intergenic 45.0%
CAACAATCTTATCGGAGGAC+AGG - chr5.3:80470119-80470138 None:intergenic 45.0%
CGGAAGAATAAACAGGTGGT+GGG - chr5.3:80469411-80469430 None:intergenic 45.0%
CTAGAATTCCAGACATGGTG+CGG - chr5.3:80470036-80470055 None:intergenic 45.0%
CTTCGGGAAAAGATTGAGTG+TGG + chr5.3:80469777-80469796 MS.gene75801:CDS 45.0%
GAGAGAAAGTGAAAGACCGA+CGG - chr5.3:80469677-80469696 None:intergenic 45.0%
GCACATCATTGCTCAACACA+TGG + chr5.3:80470272-80470291 MS.gene75801:CDS 45.0%
GCCTTGACGGAAGAATAAAC+AGG - chr5.3:80469418-80469437 None:intergenic 45.0%
TGTGAGATGGATATCTCACC+GGG - chr5.3:80469550-80469569 None:intergenic 45.0%
! AAGGGTTTACCTTTGGGAAG+TGG + chr5.3:80469700-80469719 MS.gene75801:CDS 45.0%
! CAGCACTGCCTTTAGAGATA+GGG + chr5.3:80470007-80470026 MS.gene75801:CDS 45.0%
! CAGGGGGATTTGTTCATGTT+AGG + chr5.3:80470087-80470106 MS.gene75801:CDS 45.0%
!! AGAATAAACAGGTGGTGGGT+CGG - chr5.3:80469407-80469426 None:intergenic 45.0%
!! TCGAATGCGGAGCATTTTCA+TGG - chr5.3:80469653-80469672 None:intergenic 45.0%
AAAGACCGACGGATCGAATG+CGG - chr5.3:80469666-80469685 None:intergenic 50.0%
AAGAAGGCTGCCATTCAAGC+TGG + chr5.3:80470180-80470199 MS.gene75801:CDS 50.0%
ATACCAGCGCAGTTCCAAAG+AGG - chr5.3:80469616-80469635 None:intergenic 50.0%
ATGTTGCCCCATCCATCATG+GGG + chr5.3:80469860-80469879 MS.gene75801:CDS 50.0%
CAACACAAAACCCCCCATGA+TGG - chr5.3:80469875-80469894 None:intergenic 50.0%
CAATGTTGCCCCATCCATCA+TGG + chr5.3:80469858-80469877 MS.gene75801:CDS 50.0%
CCTGGCTAGAATTCCAGACA+TGG - chr5.3:80470041-80470060 None:intergenic 50.0%
CTTGTGGCGTCAGTGTTACA+GGG + chr5.3:80470069-80470088 MS.gene75801:CDS 50.0%
GATCCCAAACCACTTCCCAA+AGG - chr5.3:80469712-80469731 None:intergenic 50.0%
GATCTCATTGACAGCAACCG+CGG - chr5.3:80469758-80469777 None:intergenic 50.0%
GCATCCATCCCAGGAATCAA+TGG - chr5.3:80470156-80470175 None:intergenic 50.0%
GCCAAAAGCTCCAGCTTGAA+TGG - chr5.3:80470193-80470212 None:intergenic 50.0%
GCGATCAAGCTGCTTGACAT+TGG - chr5.3:80470337-80470356 None:intergenic 50.0%
GGAATCAATGGTGCACGCTT+AGG - chr5.3:80470144-80470163 None:intergenic 50.0%
GTGAGATGGATATCTCACCG+GGG - chr5.3:80469549-80469568 None:intergenic 50.0%
TACCAGCGCAGTTCCAAAGA+GGG - chr5.3:80469615-80469634 None:intergenic 50.0%
TCTTGTGGCGTCAGTGTTAC+AGG + chr5.3:80470068-80470087 MS.gene75801:CDS 50.0%
TGACACTGTCTCCGTCAAAG+TGG + chr5.3:80469510-80469529 MS.gene75801:CDS 50.0%
TGCGGCATCCCTATCTCTAA+AGG - chr5.3:80470018-80470037 None:intergenic 50.0%
TGGGATGGATGCTGTCAAGA+AGG + chr5.3:80470164-80470183 MS.gene75801:CDS 50.0%
TTCTTGACAGCATCCATCCC+AGG - chr5.3:80470165-80470184 None:intergenic 50.0%
TTGTGGCGTCAGTGTTACAG+GGG + chr5.3:80470070-80470089 MS.gene75801:CDS 50.0%
! ACAAAACCCCCCATGATGGA+TGG - chr5.3:80469871-80469890 None:intergenic 50.0%
! CCATGTCTGGAATTCTAGCC+AGG + chr5.3:80470038-80470057 MS.gene75801:CDS 50.0%
! GCAGCACTGCCTTTAGAGAT+AGG + chr5.3:80470006-80470025 MS.gene75801:CDS 50.0%
! GCCATTCAAGCTGGAGCTTT+TGG + chr5.3:80470189-80470208 MS.gene75801:CDS 50.0%
! GGTTGCTGTCACTGATAACG+AGG + chr5.3:80470242-80470261 MS.gene75801:CDS 50.0%
!! AAAACCCCCCATGATGGATG+GGG - chr5.3:80469869-80469888 None:intergenic 50.0%
!! AAGCGTGCACCATTGATTCC+TGG + chr5.3:80470144-80470163 MS.gene75801:CDS 50.0%
!! AGCGTGCACCATTGATTCCT+GGG + chr5.3:80470145-80470164 MS.gene75801:CDS 50.0%
!! CAAAACCCCCCATGATGGAT+GGG - chr5.3:80469870-80469889 None:intergenic 50.0%
!! TGCACCATTGATTCCTGGGA+TGG + chr5.3:80470149-80470168 MS.gene75801:CDS 50.0%
AGTAGGACCAGCACCACTGA+TGG - chr5.3:80470220-80470239 None:intergenic 55.0%
ATCGAAGCCGGGACCTAGAT+TGG - chr5.3:80469461-80469480 None:intergenic 55.0%
ATCTCACCGGGGTGAACATC+TGG - chr5.3:80469538-80469557 None:intergenic 55.0%
ATGCTCCGCATTCGATCCGT+CGG + chr5.3:80469658-80469677 MS.gene75801:CDS 55.0%
CAGCCAAGGAAATCGAAGCC+GGG - chr5.3:80469472-80469491 None:intergenic 55.0%
GCAGCCAAGGAAATCGAAGC+CGG - chr5.3:80469473-80469492 None:intergenic 55.0%
GGAGGGATGCTTCTTCTGAG+AGG - chr5.3:80469371-80469390 None:intergenic 55.0%
GTCAAGCAGCTTGATCGCCT+TGG + chr5.3:80470339-80470358 MS.gene75801:CDS 55.0%
TCAGTGACAGCAACCGCAGT+AGG - chr5.3:80470237-80470256 None:intergenic 55.0%
TCGGTAAGTGTGACGACGGA+GGG - chr5.3:80469388-80469407 None:intergenic 55.0%
TCTCACCGGGGTGAACATCT+GGG - chr5.3:80469537-80469556 None:intergenic 55.0%
TGGGTCGGTAAGTGTGACGA+CGG - chr5.3:80469392-80469411 None:intergenic 55.0%
TGTGGCGTCAGTGTTACAGG+GGG + chr5.3:80470071-80470090 MS.gene75801:CDS 55.0%
TGTTGCCCCATCCATCATGG+GGG + chr5.3:80469861-80469880 MS.gene75801:CDS 55.0%
!! AACCCTCTTTGGAACTGCGC+TGG + chr5.3:80469610-80469629 MS.gene75801:CDS 55.0%
!! GCTTTTGGCTGCACCATCAG+TGG + chr5.3:80470204-80470223 MS.gene75801:CDS 55.0%
ACCGTCGCCAATCTAGGTCC+CGG + chr5.3:80469451-80469470 MS.gene75801:CDS 60.0%
GGCAGGAGCAAAGGCCTTGA+CGG - chr5.3:80469431-80469450 None:intergenic 60.0%
GGTCCCGGCTTCGATTTCCT+TGG + chr5.3:80469466-80469485 MS.gene75801:CDS 60.0%
GTCGGTAAGTGTGACGACGG+AGG - chr5.3:80469389-80469408 None:intergenic 60.0%
GTGGACCCAGATGTTCACCC+CGG + chr5.3:80469529-80469548 MS.gene75801:CDS 60.0%
GTTGCCCCATCCATCATGGG+GGG + chr5.3:80469862-80469881 MS.gene75801:CDS 60.0%
TAGCCAGGCAGCTGCTCTTG+TGG + chr5.3:80470053-80470072 MS.gene75801:CDS 60.0%
TAGGGATGCCGCACCATGTC+TGG + chr5.3:80470025-80470044 MS.gene75801:CDS 60.0%
TTCCTTGGCTGCGCTGTCGA+TGG + chr5.3:80469481-80469500 MS.gene75801:CDS 60.0%
!! CAGTGGTGCTGGTCCTACTG+CGG + chr5.3:80470221-80470240 MS.gene75801:CDS 60.0%
ACGCCACAAGAGCAGCTGCC+TGG - chr5.3:80470059-80470078 None:intergenic 65.0%
CCTAGATTGGCGACGGTGGC+AGG - chr5.3:80469448-80469467 None:intergenic 65.0%
CCTGCCACCGTCGCCAATCT+AGG + chr5.3:80469445-80469464 MS.gene75801:CDS 65.0%
GCCGGGACCTAGATTGGCGA+CGG - chr5.3:80469455-80469474 None:intergenic 65.0%
GGCTGCACCATCAGTGGTGC+TGG + chr5.3:80470210-80470229 MS.gene75801:CDS 65.0%
GGCTGCGCTGTCGATGGCAT+CGG + chr5.3:80469487-80469506 MS.gene75801:CDS 65.0%
GGGACCTAGATTGGCGACGG+TGG - chr5.3:80469452-80469471 None:intergenic 65.0%
TGCCATCGACAGCGCAGCCA+AGG - chr5.3:80469486-80469505 None:intergenic 65.0%
GACAGCAACCGCGGCTGCAG+CGG - chr5.3:80469749-80469768 None:intergenic 70.0%
GGCGACGGTGGCAGGAGCAA+AGG - chr5.3:80469440-80469459 None:intergenic 70.0%
!! TGCAGCGGCGCTGGCAGCAC+TGG - chr5.3:80469734-80469753 None:intergenic 75.0%
! AACCGCGGCTGCAGCGGCGC+TGG - chr5.3:80469743-80469762 None:intergenic 80.0%
! TGCCAGCGCCGCTGCAGCCG+CGG + chr5.3:80469738-80469757 MS.gene75801:CDS 80.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 80469250 80470395 80469250 ID=MS.gene75801
chr5.3 mRNA 80469250 80470395 80469250 ID=MS.gene75801.t1;Parent=MS.gene75801
chr5.3 exon 80469250 80470395 80469250 ID=MS.gene75801.t1.exon1;Parent=MS.gene75801.t1
chr5.3 CDS 80469250 80470395 80469250 ID=cds.MS.gene75801.t1;Parent=MS.gene75801.t1
Gene Sequence

>MS.gene75801

ATGGCTGCTTCAACAACATGCTTTTTTCAGTCTTCTTCATCTCCACTTTCCTTTAATTTCAAACAAAAGAATAATCAAAATATTCATATTCATATTTCAATTTTTAGAATCAGATGCTCCTCTCAGAAGAAGCATCCCTCCGTCGTCACACTTACCGACCCACCACCTGTTTATTCTTCCGTCAAGGCCTTTGCTCCTGCCACCGTCGCCAATCTAGGTCCCGGCTTCGATTTCCTTGGCTGCGCTGTCGATGGCATCGGTGACACTGTCTCCGTCAAAGTGGACCCAGATGTTCACCCCGGTGAGATATCCATCTCACATATTTCTGGTCAAACACCTAACATCCACAAACTCAGTAAAAACCCTCTTTGGAACTGCGCTGGTATCGCCGCTATCGAAGCCATGAAAATGCTCCGCATTCGATCCGTCGGTCTTTCACTTTCTCTCGAAAAGGGTTTACCTTTGGGAAGTGGTTTGGGATCCAGTGCTGCCAGCGCCGCTGCAGCCGCGGTTGCTGTCAATGAGATCTTCGGGAAAAGATTGAGTGTGGATGACCTTGTTCTCGCCTGCTTGAAATCAGAAGAGAAGGTTTCTGGTTATCACGCAGACAATGTTGCCCCATCCATCATGGGGGGTTTTGTGTTGATTCGCAATTACCAACCTTTGGAATTGATGCCGTTGAAGTTCCCATCTGAAAAACAGCTATATTTCGTATTAGTTACACCAGAATTTGAAGCCCCAACTAAAAAAATGAGAGCAGCACTGCCTTTAGAGATAGGGATGCCGCACCATGTCTGGAATTCTAGCCAGGCAGCTGCTCTTGTGGCGTCAGTGTTACAGGGGGATTTGTTCATGTTAGGGAAGGCCCTGTCCTCCGATAAGATTGTTGAACCTAAGCGTGCACCATTGATTCCTGGGATGGATGCTGTCAAGAAGGCTGCCATTCAAGCTGGAGCTTTTGGCTGCACCATCAGTGGTGCTGGTCCTACTGCGGTTGCTGTCACTGATAACGAGGAAAAGGGGCACATCATTGCTCAACACATGGTTCAAGCTTTTCAAAAGGAAGGAAACTTGAATGCTTCTGCCAATGTCAAGCAGCTTGATCGCCTTGGTGCTAGACTTATTAGCAGCGTTCATTTAAATTGA
Protein sequence

>MS.gene75801.t1

MAASTTCFFQSSSSPLSFNFKQKNNQNIHIHISIFRIRCSSQKKHPSVVTLTDPPPVYSSVKAFAPATVANLGPGFDFLGCAVDGIGDTVSVKVDPDVHPGEISISHISGQTPNIHKLSKNPLWNCAGIAAIEAMKMLRIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNEIFGKRLSVDDLVLACLKSEEKVSGYHADNVAPSIMGGFVLIRNYQPLELMPLKFPSEKQLYFVLVTPEFEAPTKKMRAALPLEIGMPHHVWNSSQAAALVASVLQGDLFMLGKALSSDKIVEPKRAPLIPGMDAVKKAAIQAGAFGCTISGAGPTAVAVTDNEEKGHIIAQHMVQAFQKEGNLNASANVKQLDRLGARLISSVHLN