Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76240.t1 | OMO84785.1 | 96.7 | 91 | 3 | 0 | 33 | 123 | 41 | 131 | 7.80E-44 | 186.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76240.t1 | Q9M2F1 | 96.5 | 86 | 3 | 0 | 38 | 123 | 1 | 86 | 3.0e-44 | 179.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76240.t1 | A0A1R3IQB9 | 96.7 | 91 | 3 | 0 | 33 | 123 | 41 | 131 | 5.6e-44 | 186.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05491 | MS.gene76240 | 0.845332 | 4.34E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76240.t1 | MTR_2g029270 | 100.000 | 86 | 0 | 0 | 38 | 123 | 1 | 86 | 1.29e-60 | 181 |
| MS.gene76240.t1 | MTR_1g111970 | 100.000 | 86 | 0 | 0 | 38 | 123 | 1 | 86 | 1.29e-60 | 181 |
| MS.gene76240.t1 | MTR_2g015680 | 98.837 | 86 | 1 | 0 | 38 | 123 | 1 | 86 | 3.36e-60 | 179 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76240.t1 | AT3G61110 | 96.512 | 86 | 3 | 0 | 38 | 123 | 1 | 86 | 1.65e-59 | 178 |
| MS.gene76240.t1 | AT2G45710 | 95.238 | 84 | 4 | 0 | 38 | 121 | 1 | 84 | 1.63e-56 | 170 |
| MS.gene76240.t1 | AT5G47930 | 96.429 | 84 | 3 | 0 | 38 | 121 | 1 | 84 | 5.46e-56 | 169 |
| MS.gene76240.t1 | AT3G61111 | 83.721 | 86 | 14 | 0 | 38 | 123 | 1 | 86 | 4.24e-49 | 152 |
| MS.gene76240.t1 | AT3G61111 | 80.952 | 42 | 8 | 0 | 38 | 79 | 1 | 42 | 3.56e-18 | 73.6 |
Find 38 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATCTCATCAAGAGTTTCT+AGG | 0.216191 | 1.1:+79025854 | MS.gene76240:CDS |
| TCCACAGCCCTCTCAACTTT+GGG | 0.311830 | 1.1:-79025676 | None:intergenic |
| TAACAGAGGGGTGCTCCTTC+AGG | 0.320285 | 1.1:+79026944 | MS.gene76240:CDS |
| ATCTCCTAACTCATTCTTCA+TGG | 0.338607 | 1.1:+79026086 | MS.gene76240:CDS |
| TTGCTTTGCCACCGGTTGGC+TGG | 0.350949 | 1.1:-79026918 | None:intergenic |
| TCAAGAGATCGATGTCGTTT+TGG | 0.351397 | 1.1:-79026011 | None:intergenic |
| TTCCACAGCCCTCTCAACTT+TGG | 0.365114 | 1.1:-79025677 | None:intergenic |
| CTAGAAACTCTTGATGAGAT+TGG | 0.393319 | 1.1:-79025853 | None:intergenic |
| AATACCATGAAGAATGAGTT+AGG | 0.426658 | 1.1:-79026090 | None:intergenic |
| CTTACATGTTGAAGCATCCT+TGG | 0.445045 | 1.1:-79026225 | None:intergenic |
| AATCTCATCAAGAGTTTCTA+GGG | 0.454646 | 1.1:+79025855 | MS.gene76240:CDS |
| TGTTAGCCTTGCTTTGCCAC+CGG | 0.490848 | 1.1:-79026926 | None:intergenic |
| CACCAGCGGAGCTTGAGAAA+AGG | 0.496318 | 1.1:+79026037 | MS.gene76240:CDS |
| AGCCAACCGGTGGCAAAGCA+AGG | 0.501626 | 1.1:+79026920 | MS.gene76240:CDS |
| GGGGTGCTCCTTCAGGAAGA+AGG | 0.503456 | 1.1:+79026951 | MS.gene76240:CDS |
| TCTCAAACAGTCGTGGTTTG+CGG | 0.511373 | 1.1:+79026874 | MS.gene76240:CDS |
| AGCCTTGCTTTGCCACCGGT+TGG | 0.526124 | 1.1:-79026922 | None:intergenic |
| TGTTTGAGAGTGGCTAAATA+CGG | 0.536688 | 1.1:-79026860 | None:intergenic |
| AACTCTTGATGAGATTGGAA+GGG | 0.554781 | 1.1:-79025848 | None:intergenic |
| AAACTCTTGATGAGATTGGA+AGG | 0.561575 | 1.1:-79025849 | None:intergenic |
| TTATAGGATGTCAAATGCCA+AGG | 0.569104 | 1.1:+79026208 | MS.gene76240:intron |
| ACTCTTGATGAGATTGGAAG+GGG | 0.572530 | 1.1:-79025847 | None:intergenic |
| GGGTGCTCCTTCAGGAAGAA+GGG | 0.581280 | 1.1:+79026952 | MS.gene76240:CDS |
| GGTTTGCGGCAACTGTCAGA+CGG | 0.592548 | 1.1:+79026888 | MS.gene76240:CDS |
| GGCAAAGCAAGGCTAACAGA+GGG | 0.595874 | 1.1:+79026931 | MS.gene76240:CDS |
| GATGGGACCCAAAGTTGAGA+GGG | 0.597213 | 1.1:+79025669 | None:intergenic |
| ACCCAAAGTTGAGAGGGCTG+TGG | 0.599911 | 1.1:+79025675 | MS.gene76240:CDS |
| CTAATCGCCCTTCTTCCTGA+AGG | 0.607238 | 1.1:-79026959 | None:intergenic |
| AGATGGGACCCAAAGTTGAG+AGG | 0.608186 | 1.1:+79025668 | None:intergenic |
| TAGCCACTCTCAAACAGTCG+TGG | 0.620995 | 1.1:+79026867 | MS.gene76240:CDS |
| GGCTGTGGAATCAAATGTGG+AGG | 0.628873 | 1.1:+79025690 | MS.gene76240:CDS |
| TCATCTCTCAACCTCGACAA+TGG | 0.631221 | 1.1:+79025891 | MS.gene76240:CDS |
| AGAGTGGTTAACCATTGTCG+AGG | 0.650113 | 1.1:-79025902 | None:intergenic |
| TGGCAAAGCAAGGCTAACAG+AGG | 0.657490 | 1.1:+79026930 | MS.gene76240:CDS |
| AAACCACGACTGTTTGAGAG+TGG | 0.670924 | 1.1:-79026870 | None:intergenic |
| GAGGGCTGTGGAATCAAATG+TGG | 0.673501 | 1.1:+79025687 | MS.gene76240:CDS |
| CGATCTCTTGAACCCACCAG+CGG | 0.674494 | 1.1:+79026023 | MS.gene76240:CDS |
| GCAAAGCAAGGCTAACAGAG+GGG | 0.737903 | 1.1:+79026932 | MS.gene76240:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAACAAATTACAAA+CGG | - | chr1.1:79026162-79026181 | None:intergenic | 10.0% |
| !!! | TTATTATATTTCTATCATTT+AGG | + | chr1.1:79026568-79026587 | MS.gene76240:intron | 10.0% |
| !! | AATTAACATATACATAACAA+TGG | - | chr1.1:79026131-79026150 | None:intergenic | 15.0% |
| !! | AGTTTATTCAACTAAAAAAA+TGG | - | chr1.1:79026381-79026400 | None:intergenic | 15.0% |
| !! | ATGACTAAATTAATAGAATT+CGG | - | chr1.1:79026448-79026467 | None:intergenic | 15.0% |
| !! | TAAATGGAAAATCTATTTAA+AGG | - | chr1.1:79025793-79025812 | None:intergenic | 15.0% |
| !! | TTTATAATTGTTGAAATTTG+TGG | + | chr1.1:79026826-79026845 | MS.gene76240:intron | 15.0% |
| !!! | AAATTACTTTTTTATTCAAC+CGG | - | chr1.1:79026320-79026339 | None:intergenic | 15.0% |
| !! | AAAAGACTGTAAATGTTAAA+TGG | - | chr1.1:79025809-79025828 | None:intergenic | 20.0% |
| !! | AATTGAAACCAACTTATTAT+AGG | + | chr1.1:79026780-79026799 | MS.gene76240:intron | 20.0% |
| !! | CAAACTTAATCAATCATAAT+TGG | - | chr1.1:79025967-79025986 | None:intergenic | 20.0% |
| !! | CATCAATTTAATCTATGTTA+GGG | - | chr1.1:79026486-79026505 | None:intergenic | 20.0% |
| !! | TGACTAAATTAATAGAATTC+GGG | - | chr1.1:79026447-79026466 | None:intergenic | 20.0% |
| !! | TTATTATACTTATACAACTC+AGG | - | chr1.1:79026419-79026438 | None:intergenic | 20.0% |
| !! | TTTATAATGGTTGAAATTTG+TGG | + | chr1.1:79026701-79026720 | MS.gene76240:intron | 20.0% |
| !!! | AATTACTTTTTTATTCAACC+GGG | - | chr1.1:79026319-79026338 | None:intergenic | 20.0% |
| !!! | ATATCATATCTTTTTCACAT+GGG | + | chr1.1:79025737-79025756 | MS.gene76240:intron | 20.0% |
| !!! | ATTTTTATCGTTTAGGAATT+AGG | + | chr1.1:79026518-79026537 | MS.gene76240:intron | 20.0% |
| !!! | TATATCATATCTTTTTCACA+TGG | + | chr1.1:79025736-79025755 | MS.gene76240:intron | 20.0% |
| !!! | TATGCATATTTTTATCGTTT+AGG | + | chr1.1:79026511-79026530 | MS.gene76240:intron | 20.0% |
| !!! | TTTCTCTTTGTATTTGTTAT+AGG | + | chr1.1:79026192-79026211 | MS.gene76240:intron | 20.0% |
| !!! | TTTGACTTGTTAGTTTATAA+TGG | + | chr1.1:79026688-79026707 | MS.gene76240:intron | 20.0% |
| ! | CCATCAATTTAATCTATGTT+AGG | - | chr1.1:79026487-79026506 | None:intergenic | 25.0% |
| ! | CCTAACATAGATTAAATTGA+TGG | + | chr1.1:79026484-79026503 | MS.gene76240:intron | 25.0% |
| !! | AAATTTGACTTCTACTTTGA+AGG | - | chr1.1:79026752-79026771 | None:intergenic | 25.0% |
| !! | AATTTGACTTCTACTTTGAA+GGG | - | chr1.1:79026751-79026770 | None:intergenic | 25.0% |
| !!! | TTGATGATTTTGTTGTTTTC+AGG | + | chr1.1:79025984-79026003 | MS.gene76240:intron | 25.0% |
| AAACCAACAAGTCAAATCAA+AGG | - | chr1.1:79026809-79026828 | None:intergenic | 30.0% | |
| AATACCATGAAGAATGAGTT+AGG | - | chr1.1:79026093-79026112 | None:intergenic | 30.0% | |
| AATCTCATCAAGAGTTTCTA+GGG | + | chr1.1:79025855-79025874 | MS.gene76240:CDS | 30.0% | |
| ACCAAAAATTCGACTTATAC+AGG | - | chr1.1:79026632-79026651 | None:intergenic | 30.0% | |
| CAATGTCACAATGATTGTTA+TGG | + | chr1.1:79026543-79026562 | MS.gene76240:intron | 30.0% | |
| TAAAGAAACAAAAGAGAGAG+TGG | - | chr1.1:79025921-79025940 | None:intergenic | 30.0% | |
| TTATATAGATACTAGAGACC+CGG | + | chr1.1:79026298-79026317 | MS.gene76240:intron | 30.0% | |
| ! | CTTTGAAGGGAAAATAAATG+TGG | - | chr1.1:79026738-79026757 | None:intergenic | 30.0% |
| ! | TCCTGTATAAGTCGAATTTT+TGG | + | chr1.1:79026628-79026647 | MS.gene76240:intron | 30.0% |
| !! | AAAGGTGTCCTATAATAAGT+TGG | - | chr1.1:79026791-79026810 | None:intergenic | 30.0% |
| AAACTCTTGATGAGATTGGA+AGG | - | chr1.1:79025852-79025871 | None:intergenic | 35.0% | |
| AACTCTTGATGAGATTGGAA+GGG | - | chr1.1:79025851-79025870 | None:intergenic | 35.0% | |
| ATCTCCTAACTCATTCTTCA+TGG | + | chr1.1:79026086-79026105 | MS.gene76240:CDS | 35.0% | |
| CAATCATAATTGGAGCGATT+CGG | - | chr1.1:79025957-79025976 | None:intergenic | 35.0% | |
| CAATCTCATCAAGAGTTTCT+AGG | + | chr1.1:79025854-79025873 | MS.gene76240:CDS | 35.0% | |
| CTAGAAACTCTTGATGAGAT+TGG | - | chr1.1:79025856-79025875 | None:intergenic | 35.0% | |
| TGTTTGAGAGTGGCTAAATA+CGG | - | chr1.1:79026863-79026882 | None:intergenic | 35.0% | |
| TTATAGGATGTCAAATGCCA+AGG | + | chr1.1:79026208-79026227 | MS.gene76240:intron | 35.0% | |
| ! | TTTTTCACATGGGCTCTTAA+TGG | + | chr1.1:79025747-79025766 | MS.gene76240:intron | 35.0% |
| !! | ACACCTTTGATTTGACTTGT+TGG | + | chr1.1:79026803-79026822 | MS.gene76240:intron | 35.0% |
| ACTCTTGATGAGATTGGAAG+GGG | - | chr1.1:79025850-79025869 | None:intergenic | 40.0% | |
| ! | CTTACATGTTGAAGCATCCT+TGG | - | chr1.1:79026228-79026247 | None:intergenic | 40.0% |
| !! | TCAAGAGATCGATGTCGTTT+TGG | - | chr1.1:79026014-79026033 | None:intergenic | 40.0% |
| AAACCACGACTGTTTGAGAG+TGG | - | chr1.1:79026873-79026892 | None:intergenic | 45.0% | |
| AAGGCGACTCTCACTCATAT+TGG | - | chr1.1:79025774-79025793 | None:intergenic | 45.0% | |
| AGAGTGGTTAACCATTGTCG+AGG | - | chr1.1:79025905-79025924 | None:intergenic | 45.0% | |
| AGGCGACTCTCACTCATATT+GGG | - | chr1.1:79025773-79025792 | None:intergenic | 45.0% | |
| TCATCTCTCAACCTCGACAA+TGG | + | chr1.1:79025891-79025910 | MS.gene76240:CDS | 45.0% | |
| TCTCAAACAGTCGTGGTTTG+CGG | + | chr1.1:79026874-79026893 | MS.gene76240:CDS | 45.0% | |
| ! | TCATAATTGGAGCGATTCGG+AGG | - | chr1.1:79025954-79025973 | None:intergenic | 45.0% |
| CTAATCGCCCTTCTTCCTGA+AGG | - | chr1.1:79026962-79026981 | None:intergenic | 50.0% | |
| GAGGGCTGTGGAATCAAATG+TGG | + | chr1.1:79025687-79025706 | MS.gene76240:CDS | 50.0% | |
| GCAAAGCAAGGCTAACAGAG+GGG | + | chr1.1:79026932-79026951 | MS.gene76240:CDS | 50.0% | |
| GGCAAAGCAAGGCTAACAGA+GGG | + | chr1.1:79026931-79026950 | MS.gene76240:CDS | 50.0% | |
| GGCTGTGGAATCAAATGTGG+AGG | + | chr1.1:79025690-79025709 | MS.gene76240:CDS | 50.0% | |
| TAGCCACTCTCAAACAGTCG+TGG | + | chr1.1:79026867-79026886 | MS.gene76240:CDS | 50.0% | |
| TCCACAGCCCTCTCAACTTT+GGG | - | chr1.1:79025679-79025698 | None:intergenic | 50.0% | |
| TGGCAAAGCAAGGCTAACAG+AGG | + | chr1.1:79026930-79026949 | MS.gene76240:CDS | 50.0% | |
| TTCCACAGCCCTCTCAACTT+TGG | - | chr1.1:79025680-79025699 | None:intergenic | 50.0% | |
| ! | TTCCTTTTCTCAAGCTCCGC+TGG | - | chr1.1:79026042-79026061 | None:intergenic | 50.0% |
| ! | TTTTCTCAAGCTCCGCTGGT+GGG | - | chr1.1:79026038-79026057 | None:intergenic | 50.0% |
| !! | TGTTAGCCTTGCTTTGCCAC+CGG | - | chr1.1:79026929-79026948 | None:intergenic | 50.0% |
| ACCCAAAGTTGAGAGGGCTG+TGG | + | chr1.1:79025675-79025694 | MS.gene76240:CDS | 55.0% | |
| CACCAGCGGAGCTTGAGAAA+AGG | + | chr1.1:79026037-79026056 | MS.gene76240:CDS | 55.0% | |
| CGATCTCTTGAACCCACCAG+CGG | + | chr1.1:79026023-79026042 | MS.gene76240:CDS | 55.0% | |
| GGGTGCTCCTTCAGGAAGAA+GGG | + | chr1.1:79026952-79026971 | MS.gene76240:CDS | 55.0% | |
| GGTTTGCGGCAACTGTCAGA+CGG | + | chr1.1:79026888-79026907 | MS.gene76240:CDS | 55.0% | |
| ! | CTTTTCTCAAGCTCCGCTGG+TGG | - | chr1.1:79026039-79026058 | None:intergenic | 55.0% |
| !! | ACGGTTTTGTGCCAGCCAAC+CGG | + | chr1.1:79026907-79026926 | MS.gene76240:CDS | 55.0% |
| !! | TAACAGAGGGGTGCTCCTTC+AGG | + | chr1.1:79026944-79026963 | MS.gene76240:CDS | 55.0% |
| AGCCAACCGGTGGCAAAGCA+AGG | + | chr1.1:79026920-79026939 | MS.gene76240:CDS | 60.0% | |
| AGCCTTGCTTTGCCACCGGT+TGG | - | chr1.1:79026925-79026944 | None:intergenic | 60.0% | |
| ! | GGGGTGCTCCTTCAGGAAGA+AGG | + | chr1.1:79026951-79026970 | MS.gene76240:CDS | 60.0% |
| ! | GTTTTGTGCCAGCCAACCGG+TGG | + | chr1.1:79026910-79026929 | MS.gene76240:CDS | 60.0% |
| ! | TTGCTTTGCCACCGGTTGGC+TGG | - | chr1.1:79026921-79026940 | None:intergenic | 60.0% |
| AAGGGGCTGCGACTGCGAGA+AGG | - | chr1.1:79025833-79025852 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 79025670 | 79026981 | 79025670 | ID=MS.gene76240 |
| chr1.1 | mRNA | 79025670 | 79026981 | 79025670 | ID=MS.gene76240.t1;Parent=MS.gene76240 |
| chr1.1 | exon | 79025670 | 79025711 | 79025670 | ID=MS.gene76240.t1.exon1;Parent=MS.gene76240.t1 |
| chr1.1 | CDS | 79025670 | 79025711 | 79025670 | ID=cds.MS.gene76240.t1;Parent=MS.gene76240.t1 |
| chr1.1 | exon | 79025841 | 79025912 | 79025841 | ID=MS.gene76240.t1.exon2;Parent=MS.gene76240.t1 |
| chr1.1 | CDS | 79025841 | 79025912 | 79025841 | ID=cds.MS.gene76240.t1;Parent=MS.gene76240.t1 |
| chr1.1 | exon | 79026006 | 79026107 | 79026006 | ID=MS.gene76240.t1.exon3;Parent=MS.gene76240.t1 |
| chr1.1 | CDS | 79026006 | 79026107 | 79026006 | ID=cds.MS.gene76240.t1;Parent=MS.gene76240.t1 |
| chr1.1 | exon | 79026214 | 79026242 | 79026214 | ID=MS.gene76240.t1.exon4;Parent=MS.gene76240.t1 |
| chr1.1 | CDS | 79026214 | 79026242 | 79026214 | ID=cds.MS.gene76240.t1;Parent=MS.gene76240.t1 |
| chr1.1 | exon | 79026855 | 79026981 | 79026855 | ID=MS.gene76240.t1.exon5;Parent=MS.gene76240.t1 |
| chr1.1 | CDS | 79026855 | 79026981 | 79026855 | ID=cds.MS.gene76240.t1;Parent=MS.gene76240.t1 |
| Gene Sequence |
| Protein sequence |
