Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76858.t1 | XP_003614060.2 | 96.4 | 604 | 22 | 0 | 1 | 604 | 1 | 604 | 0.00E+00 | 1163.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76858.t1 | Q9FZD1 | 62.8 | 573 | 202 | 3 | 39 | 600 | 51 | 623 | 2.4e-211 | 736.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76858.t1 | G7JWU3 | 96.4 | 604 | 22 | 0 | 1 | 604 | 1 | 604 | 0.0e+00 | 1163.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene76858 | MS.gene35108 | PPI |
| MS.gene76858 | MS.gene28721 | PPI |
| MS.gene76858 | MS.gene028350 | PPI |
| MS.gene76858 | MS.gene36441 | PPI |
| MS.gene35110 | MS.gene76858 | PPI |
| MS.gene76858 | MS.gene064542 | PPI |
| MS.gene76858 | MS.gene73119 | PPI |
| MS.gene035805 | MS.gene76858 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76858.t1 | MTR_5g044270 | 96.358 | 604 | 22 | 0 | 1 | 604 | 1 | 604 | 0.0 | 1209 |
| MS.gene76858.t1 | MTR_6g024415 | 95.413 | 109 | 5 | 0 | 287 | 395 | 3 | 111 | 1.99e-65 | 212 |
| MS.gene76858.t1 | MTR_1g009040 | 60.000 | 155 | 39 | 4 | 5 | 158 | 1 | 133 | 1.15e-49 | 169 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76858.t1 | AT1G26460 | 60.835 | 623 | 221 | 7 | 1 | 600 | 1 | 623 | 0.0 | 781 |
Find 138 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATCAATTGTTTCTACTTT+TGG | 0.135748 | 5.2:-34622102 | MS.gene76858:CDS |
| ATTTGTTTAATCATTATATT+AGG | 0.156430 | 5.2:-34625496 | MS.gene76858:CDS |
| CTATTATGGCCTGGAGTTTA+TGG | 0.184716 | 5.2:-34622889 | MS.gene76858:CDS |
| GATTGATTTGATTGTGAAAT+TGG | 0.186157 | 5.2:+34625844 | None:intergenic |
| AGGAGCAGCATGGGCTGTTT+TGG | 0.238246 | 5.2:-34622751 | MS.gene76858:CDS |
| AAATTAACATCAGGTTTATT+AGG | 0.243041 | 5.2:+34625515 | None:intergenic |
| AATGGATGTAAAGAAGTAAA+TGG | 0.243768 | 5.2:+34622567 | None:intergenic |
| TGAGGGATGGGTTAGGAGTT+TGG | 0.258561 | 5.2:-34625552 | MS.gene76858:CDS |
| CCCCACTCTACAACGAAAAT+TGG | 0.270895 | 5.2:-34625724 | MS.gene76858:CDS |
| TTTGAGCATGCAACAGATAA+TGG | 0.279170 | 5.2:+34622549 | None:intergenic |
| TCCTAGCAGCTCAGAATTAT+AGG | 0.283390 | 5.2:+34622770 | None:intergenic |
| AAATTAAAACTCGCTGTATT+CGG | 0.301446 | 5.2:+34625398 | None:intergenic |
| GTTTGGGCACATAGTAAGTT+TGG | 0.305274 | 5.2:-34619013 | MS.gene76858:CDS |
| TCGGGTTCTTGATTGAGATA+TGG | 0.306238 | 5.2:+34625812 | None:intergenic |
| CATAAACTCCAGGCCATAAT+AGG | 0.307963 | 5.2:+34622890 | None:intergenic |
| AATAGCAAATTAGCCTATTA+TGG | 0.308171 | 5.2:-34622903 | MS.gene76858:CDS |
| CATGATTATGAGCAAGCTTA+TGG | 0.308715 | 5.2:-34622624 | MS.gene76858:CDS |
| CGTGAAGTTTCAAAGGTGTT+TGG | 0.315096 | 5.2:-34619030 | MS.gene76858:CDS |
| GCGAGATAAGATTCTGGATT+TGG | 0.315401 | 5.2:+34622702 | None:intergenic |
| AAACAATTGATTCAAAAGTT+TGG | 0.321687 | 5.2:+34622112 | None:intergenic |
| ATCTTTGCGAGATAAGATTC+TGG | 0.329322 | 5.2:+34622696 | None:intergenic |
| TCTGTTGGTGGTGGTGGTGT+GGG | 0.334079 | 5.2:+34625779 | None:intergenic |
| TATGAAAATGCACAATTTCC+AGG | 0.338364 | 5.2:+34622950 | None:intergenic |
| GGGGATCCTGAAGCTGGATT+TGG | 0.341174 | 5.2:+34625743 | None:intergenic |
| GATATATGCCCTTGCATCAT+TGG | 0.349384 | 5.2:-34622676 | MS.gene76858:CDS |
| ACTTCATCTTTAACCCCATT+TGG | 0.351693 | 5.2:-34625683 | MS.gene76858:CDS |
| ATTTGACATCCCGGTTAATC+AGG | 0.351971 | 5.2:+34618944 | None:intergenic |
| TGGTGTGGGGTCTACAAGTT+CGG | 0.354920 | 5.2:+34625793 | None:intergenic |
| GCGCTAAACTGTATCATTGT+AGG | 0.358136 | 5.2:-34622168 | MS.gene76858:CDS |
| CCATTGTGCGAATGAATATT+AGG | 0.358922 | 5.2:+34622072 | None:intergenic |
| GAATCATTTGACTTGGTCAT+TGG | 0.359618 | 5.2:-34623266 | MS.gene76858:CDS |
| ATTAGGGCTAATCTTATGAT+TGG | 0.367116 | 5.2:-34625479 | MS.gene76858:CDS |
| TCGATGCCCACCTGATTAAC+CGG | 0.368565 | 5.2:-34618954 | MS.gene76858:CDS |
| TATGGGAGTGCTTGATGATA+TGG | 0.373361 | 5.2:-34618917 | MS.gene76858:intron |
| AGGCAATACGACTTTGCATT+AGG | 0.374621 | 5.2:+34618982 | None:intergenic |
| TTTGGGCACATAGTAAGTTT+GGG | 0.376407 | 5.2:-34619012 | MS.gene76858:CDS |
| CGGGTTCTTGATTGAGATAT+GGG | 0.376922 | 5.2:+34625813 | None:intergenic |
| TGCATTTGGGAAGGCCAATA+AGG | 0.377932 | 5.2:-34622040 | MS.gene76858:intron |
| AGTAAGATCCATCACCTTAT+TGG | 0.380285 | 5.2:+34622026 | None:intergenic |
| ACAAAGCCCTTTGCCCCTCC+TGG | 0.381258 | 5.2:-34622968 | MS.gene76858:CDS |
| TTGTAGGATCTGCGAACATA+TGG | 0.381386 | 5.2:-34622152 | MS.gene76858:CDS |
| TTCTGTTGGTGGTGGTGGTG+TGG | 0.384006 | 5.2:+34625778 | None:intergenic |
| GATCCTGAAGCTGGATTTGG+TGG | 0.389737 | 5.2:+34625746 | None:intergenic |
| TTTGTTTAATCATTATATTA+GGG | 0.392061 | 5.2:-34625495 | MS.gene76858:CDS |
| GCTTGATTTGTTAGCTCAGA+TGG | 0.397920 | 5.2:-34625438 | MS.gene76858:CDS |
| CTCCCACCAAATCCAGCTTC+AGG | 0.401623 | 5.2:-34625749 | MS.gene76858:CDS |
| GTGAAGTTTCAAAGGTGTTT+GGG | 0.404120 | 5.2:-34619029 | MS.gene76858:CDS |
| GTTATCAGGATGCTGCAATC+TGG | 0.406078 | 5.2:-34623311 | MS.gene76858:intron |
| TCTTAATTACAGTACTGAAA+TGG | 0.406262 | 5.2:-34618652 | MS.gene76858:CDS |
| GGTTCTTAAGGCTATGCATC+AGG | 0.418948 | 5.2:-34625375 | MS.gene76858:CDS |
| AAGTCAAATGATTCATCATC+AGG | 0.420113 | 5.2:+34623275 | None:intergenic |
| AATGGGCTGATGTATGCATT+TGG | 0.421515 | 5.2:-34622054 | MS.gene76858:CDS |
| ATTTATTTGCAGATAGCTGC+TGG | 0.422592 | 5.2:-34618822 | MS.gene76858:intron |
| GGCCATCTACAAAAGGCATT+TGG | 0.424516 | 5.2:-34622654 | MS.gene76858:CDS |
| ATCTGTTGCATGCTCAAAGA+AGG | 0.425706 | 5.2:-34622544 | MS.gene76858:CDS |
| ATTGATTTGATTGTGAAATT+GGG | 0.427484 | 5.2:+34625845 | None:intergenic |
| GATGATTCTGTTGGTGGTGG+TGG | 0.436566 | 5.2:+34625773 | None:intergenic |
| ATGGATGTAAAGAAGTAAAT+GGG | 0.436831 | 5.2:+34622568 | None:intergenic |
| CACTGAAAGCGATGAAAGAC+TGG | 0.438329 | 5.2:-34618739 | MS.gene76858:CDS |
| GGTGTGGGGTCTACAAGTTC+GGG | 0.441233 | 5.2:+34625794 | None:intergenic |
| CTAATATTCATTCGCACAAT+GGG | 0.441539 | 5.2:-34622071 | MS.gene76858:CDS |
| TTGGGTAGCTTCACAACGTT+GGG | 0.445343 | 5.2:-34625594 | MS.gene76858:CDS |
| CGATCTTACAATTACCGACT+TGG | 0.449042 | 5.2:-34618705 | MS.gene76858:CDS |
| ATATATGCCCTTGCATCATT+GGG | 0.454900 | 5.2:-34622675 | MS.gene76858:CDS |
| GTGTTGCTTTCTGTAGATGA+AGG | 0.461555 | 5.2:-34622828 | MS.gene76858:CDS |
| GATTAAACAGCATGTTTCGT+CGG | 0.463811 | 5.2:+34618673 | None:intergenic |
| ATGGCTCGATGGATTGTAAA+GGG | 0.468300 | 5.2:-34622870 | MS.gene76858:CDS |
| AAATGTTTGAGGGATGGGTT+AGG | 0.468784 | 5.2:-34625559 | MS.gene76858:CDS |
| TGATTAAACAAATTAACATC+AGG | 0.470842 | 5.2:+34625506 | None:intergenic |
| GGGAGTGATGATTCTGTTGG+TGG | 0.473703 | 5.2:+34625767 | None:intergenic |
| CAATTTCCAGGAGGGGCAAA+GGG | 0.475476 | 5.2:+34622962 | None:intergenic |
| CCGGGATGTCAAATCTGCTA+TGG | 0.480468 | 5.2:-34618935 | MS.gene76858:CDS |
| AGCGCAGCAACAGACTTGTA+TGG | 0.481918 | 5.2:+34622186 | None:intergenic |
| TAGAGTGGGGATCCTGAAGC+TGG | 0.482043 | 5.2:+34625737 | None:intergenic |
| ATTAAACAGCATGTTTCGTC+GGG | 0.488375 | 5.2:+34618674 | None:intergenic |
| CAAAAGCGCGTTGGAATCGT+AGG | 0.489632 | 5.2:+34625631 | None:intergenic |
| ATGGGCTGATGTATGCATTT+GGG | 0.492973 | 5.2:-34622053 | MS.gene76858:CDS |
| TCAGGTGGGCATCGATAAGC+AGG | 0.497019 | 5.2:+34618962 | None:intergenic |
| AGTGATGATTCTGTTGGTGG+TGG | 0.500044 | 5.2:+34625770 | None:intergenic |
| CTGTTGGTGGTGGTGGTGTG+GGG | 0.502808 | 5.2:+34625780 | None:intergenic |
| CCTAATATTCATTCGCACAA+TGG | 0.508619 | 5.2:-34622072 | MS.gene76858:CDS |
| ACAAGGAAGGCTTGATACAT+TGG | 0.516589 | 5.2:-34623126 | MS.gene76858:CDS |
| GGTGGGAGTGATGATTCTGT+TGG | 0.523245 | 5.2:+34625764 | None:intergenic |
| ATCATTGGGCCATCTACAAA+AGG | 0.524550 | 5.2:-34622661 | MS.gene76858:CDS |
| GCGCGTGTGAAATGACCAAA+TGG | 0.529121 | 5.2:+34625668 | None:intergenic |
| CAAATTAGCCTATTATGGCC+TGG | 0.529386 | 5.2:-34622898 | MS.gene76858:CDS |
| AAGTTTGGTAAGCTCGATCA+AGG | 0.529861 | 5.2:+34622127 | None:intergenic |
| GAAGGAAATGTTTGAGGGAT+GGG | 0.530047 | 5.2:-34625564 | MS.gene76858:CDS |
| ACAATTTCCAGGAGGGGCAA+AGG | 0.530807 | 5.2:+34622961 | None:intergenic |
| CTGGATTTGAACCGATGAAA+GGG | 0.534065 | 5.2:-34618803 | MS.gene76858:CDS |
| GTGTCAGCTCTGATGACTGC+AGG | 0.542612 | 5.2:-34622798 | MS.gene76858:CDS |
| CGGGATGTCAAATCTGCTAT+GGG | 0.544855 | 5.2:-34618934 | MS.gene76858:CDS |
| TGGCAATAATTGAAAAGTGC+AGG | 0.545019 | 5.2:-34623103 | MS.gene76858:CDS |
| GCAGACACGTGAAGTTTCAA+AGG | 0.545561 | 5.2:-34619037 | MS.gene76858:intron |
| TGATGTGAAGGAAATGTTTG+AGG | 0.548893 | 5.2:-34625570 | MS.gene76858:CDS |
| CTGCTGAGAAACTTCTTGAA+CGG | 0.549508 | 5.2:-34625337 | MS.gene76858:intron |
| GATGTGAAGGAAATGTTTGA+GGG | 0.550779 | 5.2:-34625569 | MS.gene76858:CDS |
| GCTGGATTTGAACCGATGAA+AGG | 0.552778 | 5.2:-34618804 | MS.gene76858:CDS |
| CGATGCCCACCTGATTAACC+GGG | 0.553923 | 5.2:-34618953 | MS.gene76858:CDS |
| TCTGTTGCATGCTCAAAGAA+GGG | 0.556751 | 5.2:-34622543 | MS.gene76858:CDS |
| CTGAGCTGCTAGGAGCAGCA+TGG | 0.557087 | 5.2:-34622761 | MS.gene76858:CDS |
| TATGGCTCGATGGATTGTAA+AGG | 0.558390 | 5.2:-34622871 | MS.gene76858:CDS |
| GCCTGGAGTTTATGGCTCGA+TGG | 0.561213 | 5.2:-34622881 | MS.gene76858:CDS |
| ATCCTGAAGCTGGATTTGGT+GGG | 0.565034 | 5.2:+34625747 | None:intergenic |
| TGATGATGAATCATTTGACT+TGG | 0.565954 | 5.2:-34623273 | MS.gene76858:CDS |
| CGCGTGTGAAATGACCAAAT+GGG | 0.566353 | 5.2:+34625669 | None:intergenic |
| TGAAGGAAATGTTTGAGGGA+TGG | 0.567665 | 5.2:-34625565 | MS.gene76858:CDS |
| TGAGCTGCTAGGAGCAGCAT+GGG | 0.569572 | 5.2:-34622760 | MS.gene76858:CDS |
| TCCATCGAGCCATAAACTCC+AGG | 0.571109 | 5.2:+34622880 | None:intergenic |
| TGTAGGATCTGCGAACATAT+GGG | 0.572027 | 5.2:-34622151 | MS.gene76858:CDS |
| TGACATCCCGGTTAATCAGG+TGG | 0.581779 | 5.2:+34618947 | None:intergenic |
| GTAGATGGCCCAATGATGCA+AGG | 0.582438 | 5.2:+34622667 | None:intergenic |
| ACCTATAATTCTGAGCTGCT+AGG | 0.584992 | 5.2:-34622771 | MS.gene76858:CDS |
| ATTGGGTAGCTTCACAACGT+TGG | 0.592801 | 5.2:-34625595 | MS.gene76858:CDS |
| CTTAAGGCTATGCATCAGGC+TGG | 0.602664 | 5.2:-34625371 | MS.gene76858:CDS |
| AAAATGCACAATTTCCAGGA+GGG | 0.604881 | 5.2:+34622954 | None:intergenic |
| AGGAAGGCTTGATACATTGG+TGG | 0.606481 | 5.2:-34623123 | MS.gene76858:CDS |
| GACATCCCGGTTAATCAGGT+GGG | 0.607363 | 5.2:+34618948 | None:intergenic |
| GAAAACATTCAAAAGCGCGT+TGG | 0.609360 | 5.2:+34625622 | None:intergenic |
| ACAACGTTGGGATGATGTGA+AGG | 0.611093 | 5.2:-34625582 | MS.gene76858:CDS |
| AAATGCACAATTTCCAGGAG+GGG | 0.611198 | 5.2:+34622955 | None:intergenic |
| ACATGGTAACACGTTGTCAG+TGG | 0.611871 | 5.2:-34623183 | MS.gene76858:CDS |
| CCATAGCAGATTTGACATCC+CGG | 0.613360 | 5.2:+34618935 | None:intergenic |
| TGCAGTCATCAGAGCTGACA+CGG | 0.624877 | 5.2:+34622800 | None:intergenic |
| TAGATGGCCCAATGATGCAA+GGG | 0.631417 | 5.2:+34622668 | None:intergenic |
| GGAGTTGTGTCAAACAAGGA+AGG | 0.640031 | 5.2:-34623139 | MS.gene76858:CDS |
| AGCTGAAGTTGCAATTCGAG+CGG | 0.642238 | 5.2:-34622928 | MS.gene76858:CDS |
| GTCGGGCTTCAGAACCAAGT+CGG | 0.648802 | 5.2:+34618691 | None:intergenic |
| GCGTGTGAAATGACCAAATG+GGG | 0.653600 | 5.2:+34625670 | None:intergenic |
| ACTTGTATGGACGCTCAGCA+TGG | 0.661731 | 5.2:+34622199 | None:intergenic |
| TCATCTACAGAAAGCAACAC+AGG | 0.661970 | 5.2:+34622831 | None:intergenic |
| GGCAATAATTGAAAAGTGCA+GGG | 0.665279 | 5.2:-34623102 | MS.gene76858:intron |
| ATAGATTCAACTCTAAAACA+TGG | 0.670661 | 5.2:-34623200 | MS.gene76858:CDS |
| GTGAGGAGTTGTGTCAAACA+AGG | 0.685959 | 5.2:-34623143 | MS.gene76858:CDS |
| ATTTATTTATGAAATGTGTG+AGG | 0.693600 | 5.2:-34623160 | MS.gene76858:CDS |
| GCTGATGTATGCATTTGGGA+AGG | 0.709918 | 5.2:-34622049 | MS.gene76858:CDS |
| GAAAATGCACAATTTCCAGG+AGG | 0.712171 | 5.2:+34622953 | None:intergenic |
| TCTACAGAAAGCAACACAGG+AGG | 0.717369 | 5.2:+34622834 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.2 | gene | 34618647 | 34625918 | 34618647 | ID=MS.gene76858 |
| chr5.2 | mRNA | 34618647 | 34625918 | 34618647 | ID=MS.gene76858.t1;Parent=MS.gene76858 |
| chr5.2 | exon | 34625338 | 34625918 | 34625338 | ID=MS.gene76858.t1.exon1;Parent=MS.gene76858.t1 |
| chr5.2 | CDS | 34625338 | 34625918 | 34625338 | ID=cds.MS.gene76858.t1;Parent=MS.gene76858.t1 |
| chr5.2 | exon | 34623103 | 34623325 | 34623103 | ID=MS.gene76858.t1.exon2;Parent=MS.gene76858.t1 |
| chr5.2 | CDS | 34623103 | 34623325 | 34623103 | ID=cds.MS.gene76858.t1;Parent=MS.gene76858.t1 |
| chr5.2 | exon | 34622524 | 34623003 | 34622524 | ID=MS.gene76858.t1.exon3;Parent=MS.gene76858.t1 |
| chr5.2 | CDS | 34622524 | 34623003 | 34622524 | ID=cds.MS.gene76858.t1;Parent=MS.gene76858.t1 |
| chr5.2 | exon | 34622041 | 34622247 | 34622041 | ID=MS.gene76858.t1.exon4;Parent=MS.gene76858.t1 |
| chr5.2 | CDS | 34622041 | 34622247 | 34622041 | ID=cds.MS.gene76858.t1;Parent=MS.gene76858.t1 |
| chr5.2 | exon | 34618918 | 34619055 | 34618918 | ID=MS.gene76858.t1.exon5;Parent=MS.gene76858.t1 |
| chr5.2 | CDS | 34618918 | 34619055 | 34618918 | ID=cds.MS.gene76858.t1;Parent=MS.gene76858.t1 |
| chr5.2 | exon | 34618647 | 34618832 | 34618647 | ID=MS.gene76858.t1.exon6;Parent=MS.gene76858.t1 |
| chr5.2 | CDS | 34618647 | 34618832 | 34618647 | ID=cds.MS.gene76858.t1;Parent=MS.gene76858.t1 |
| Gene Sequence |
| Protein sequence |
