AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77090

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77090.t1 MTR_2g461920 96.914 324 10 0 6 329 216 539 0.0 643
MS.gene77090.t1 MTR_4g109770 62.691 327 95 6 6 329 168 470 3.77e-121 357
MS.gene77090.t1 MTR_5g046670 42.029 138 70 3 6 142 289 417 8.95e-18 84.3
MS.gene77090.t1 MTR_7g028740 32.057 209 92 7 6 206 122 288 4.52e-11 63.5
MS.gene77090.t1 MTR_1g053715 87.097 31 4 0 6 36 119 149 5.51e-11 63.2
MS.gene77090.t1 MTR_7g092930 60.345 58 21 2 6 61 113 170 6.36e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77090.t1 AT1G69170 51.389 72 34 1 6 77 169 239 4.74e-15 75.9
MS.gene77090.t1 AT5G50670 38.095 147 73 7 6 144 146 282 8.18e-13 68.9
MS.gene77090.t1 AT5G50670 38.095 147 73 7 6 144 146 282 8.18e-13 68.9
MS.gene77090.t1 AT5G50570 38.095 147 73 7 6 144 146 282 8.18e-13 68.9
MS.gene77090.t1 AT5G50570 38.095 147 73 7 6 144 146 282 8.18e-13 68.9
MS.gene77090.t1 AT2G42200 54.839 62 22 2 6 61 119 180 9.06e-11 62.8

Find 89 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCTTTCCTTCAAGAAAA+TGG 0.189331 2.4:-32952895 MS.gene77090:CDS
TCGATTGGCTTGAAGAATTC+TGG 0.190530 2.4:+32952662 None:intergenic
TGAGGATGTGCATTTATAAT+TGG 0.243639 2.4:+32952955 None:intergenic
CCACAAGTTGGTATTCATTC+TGG 0.255651 2.4:-32954587 MS.gene77090:CDS
ACTATCCAGGTTTCATTTGC+TGG 0.261393 2.4:-32954678 MS.gene77090:CDS
GGGAGGTTGCTTCAGTCATA+TGG 0.315390 2.4:-32954557 MS.gene77090:CDS
GCATCAGAGGCGAGCCTTGC+AGG 0.317553 2.4:-32952347 MS.gene77090:CDS
AAACATGACTCATGCTATAA+TGG 0.320615 2.4:+32952593 None:intergenic
TTACTGTTTGATATTAATAT+AGG 0.332199 2.4:+32952505 None:intergenic
TATTGTTGTACGAAGGAAAC+AGG 0.337560 2.4:+32952923 None:intergenic
ATCCAAGGAGGCTCGAATTC+CGG 0.339302 2.4:-32952817 MS.gene77090:CDS
GCTTTCTACGACGCTCATTA+TGG 0.341005 2.4:+32954609 None:intergenic
CGATTGGCTTGAAGAATTCT+GGG 0.354670 2.4:+32952663 None:intergenic
GGAGGTTGCTTCAGTCATAT+GGG 0.364253 2.4:-32954556 MS.gene77090:CDS
CCCCATCTGCCATTTCGAAA+TGG 0.368112 2.4:+32952473 None:intergenic
CACTTCCTAAGGAGGTATCT+TGG 0.394888 2.4:+32952779 None:intergenic
CCCATCTGCCATTTCGAAAT+GGG 0.406149 2.4:+32952474 None:intergenic
AGCTGTCGTAAACGTCTTGC+AGG 0.408574 2.4:-32954632 MS.gene77090:CDS
CCTAAAACCGGTTCCGCCTT+CGG 0.416837 2.4:+32952993 None:intergenic
ATAAAAGCCGAAGGCGGAAC+CGG 0.423047 2.4:-32953000 MS.gene77090:CDS
ATCCGGAATTCGAGCCTCCT+TGG 0.439031 2.4:+32952815 None:intergenic
TAATGTTTAGCTTACTATCC+AGG 0.439138 2.4:-32954691 MS.gene77090:CDS
ATCAGCTGTTCAAGGATTAT+CGG 0.450349 2.4:-32952731 MS.gene77090:CDS
GGTATTCATTCTGGAAGAGC+TGG 0.460050 2.4:-32954578 MS.gene77090:CDS
TTATTCCAAGATACCTCCTT+AGG 0.463781 2.4:-32952784 MS.gene77090:CDS
ATTGAAGTCTTCACTTCCTA+AGG 0.476688 2.4:+32952768 None:intergenic
GAAACAGAATCAGCTGTTCA+AGG 0.477684 2.4:-32952739 MS.gene77090:CDS
AGAAATGTGGAAATAGTAAC+TGG 0.480981 2.4:-32953032 MS.gene77090:CDS
AATGGTCGTGCCATCTTCAC+AGG 0.486457 2.4:+32952408 None:intergenic
ATGAGTCATGTTTCTGAAAG+TGG 0.489233 2.4:-32952583 MS.gene77090:CDS
GACCGTCTTTCCTGTGAAGA+TGG 0.490853 2.4:-32952418 MS.gene77090:CDS
CTGAAAGTGGCGTCTCGGAT+AGG 0.491794 2.4:-32952570 MS.gene77090:CDS
TGAAATCGCCCATTTCGAAA+TGG 0.493806 2.4:-32952482 MS.gene77090:CDS
TCAGCTGTTCAAGGATTATC+GGG 0.494261 2.4:-32952730 MS.gene77090:CDS
GCATGGGAATTCCTGTCGAT+TGG 0.495766 2.4:+32952647 None:intergenic
CTGATGTTAGCATGGTTCCT+TGG 0.502694 2.4:+32953103 None:intergenic
CCTCACCGGGGAGTATCTCT+TGG 0.503402 2.4:+32953067 None:intergenic
AATGGTGCTACATCTGCTAC+TGG 0.508879 2.4:-32952877 MS.gene77090:CDS
ATAATTGGAATAGAAGAAAG+AGG 0.509513 2.4:+32952970 None:intergenic
ATGATTCTTCCTGCTCTCTA+CGG 0.510890 2.4:-32952312 MS.gene77090:CDS
TCCGTCTGAGTTAAATCATA+CGG 0.511466 2.4:-32952545 MS.gene77090:CDS
CCCATTTCGAAATGGCAGAT+GGG 0.511484 2.4:-32952474 MS.gene77090:CDS
TTCAGCCAGCAAATGAAACC+TGG 0.518515 2.4:+32954673 None:intergenic
TTACGTAATCCGTAGAGAGC+AGG 0.520716 2.4:+32952303 None:intergenic
TTGCTGGCTGAATTTGATGA+TGG 0.522089 2.4:-32954662 MS.gene77090:CDS
AGAAGAAAGAGGCCTAAAAC+CGG 0.523177 2.4:+32952981 None:intergenic
TTGGAATAATGATTGTGATC+CGG 0.528558 2.4:+32952798 None:intergenic
CTATAATGGCTATGGCTGCT+CGG 0.529046 2.4:+32952607 None:intergenic
CGACAGTTGAAGCAAGTCAA+TGG 0.529505 2.4:+32952390 None:intergenic
AATAACTAAATGACCAGGCA+TGG 0.532105 2.4:+32952630 None:intergenic
GCCCATTTCGAAATGGCAGA+TGG 0.532890 2.4:-32952475 MS.gene77090:CDS
TCGACAGGAATTCCCATGCC+TGG 0.536060 2.4:-32952643 MS.gene77090:CDS
CCATTTCGAAATGGCAGATG+GGG 0.536154 2.4:-32952473 MS.gene77090:CDS
TCAGAAGATAAGACCTCACC+GGG 0.542752 2.4:+32953054 None:intergenic
GAGGTTGCTTCAGTCATATG+GGG 0.543950 2.4:-32954555 MS.gene77090:CDS
TTCGAGCCTCCTTGGATAGG+AGG 0.548698 2.4:+32952823 None:intergenic
GACTCATGCTATAATGGCTA+TGG 0.554681 2.4:+32952599 None:intergenic
CGTTGACAAAATCCGACCCC+TGG 0.557627 2.4:+32952446 None:intergenic
GTATTCATTCTGGAAGAGCT+GGG 0.561734 2.4:-32954577 MS.gene77090:CDS
GAATTCGAGCCTCCTTGGAT+AGG 0.563220 2.4:+32952820 None:intergenic
TGTTTCTGAAAGTGGCGTCT+CGG 0.565635 2.4:-32952575 MS.gene77090:CDS
AATTGTTTATTGTTGTACGA+AGG 0.567910 2.4:+32952916 None:intergenic
TCCGTATGATTTAACTCAGA+CGG 0.569364 2.4:+32952544 None:intergenic
AATTCTTCAAGCCAATCGAC+AGG 0.569495 2.4:-32952658 MS.gene77090:CDS
GAAGTCTTCACTTCCTAAGG+AGG 0.571303 2.4:+32952771 None:intergenic
CTTACAGATAGTAGATTCCA+AGG 0.572899 2.4:-32953120 MS.gene77090:intron
CTCAGAAGATAAGACCTCAC+CGG 0.577587 2.4:+32953053 None:intergenic
ATATGCCAAGAGATACTCCC+CGG 0.578163 2.4:-32953072 MS.gene77090:CDS
CTCGGAATAACTAAATGACC+AGG 0.578215 2.4:+32952625 None:intergenic
GGAAACAGGGTTCTTGAATG+AGG 0.578570 2.4:+32952937 None:intergenic
TGCCATCTTCACAGGAAAGA+CGG 0.587669 2.4:+32952416 None:intergenic
AGTCAATATCCTCCTATCCA+AGG 0.597117 2.4:-32952832 MS.gene77090:CDS
AAAAGATGCTGATGTTAGCA+TGG 0.597201 2.4:+32953095 None:intergenic
TTCAGCGTGTTGAGCATCAG+AGG 0.601537 2.4:-32952360 MS.gene77090:CDS
CGTCGTAGAAAGCCACAAGT+TGG 0.607871 2.4:-32954599 MS.gene77090:CDS
CCAGAATGAATACCAACTTG+TGG 0.611115 2.4:+32954587 None:intergenic
TCTGAGTTAAATCATACGGA+TGG 0.611214 2.4:-32952541 MS.gene77090:CDS
ATAACTAAATGACCAGGCAT+GGG 0.613150 2.4:+32952631 None:intergenic
GTCTTATCTTCTGAGAAATG+TGG 0.623862 2.4:-32953045 MS.gene77090:CDS
TGCAGACCTATAAAAGCCGA+AGG 0.654610 2.4:-32953009 MS.gene77090:CDS
GGATTATCGGGAGTATCAGA+CGG 0.658305 2.4:-32952718 MS.gene77090:CDS
ATTGTTGTACGAAGGAAACA+GGG 0.661617 2.4:+32952924 None:intergenic
CCAAGAGATACTCCCCGGTG+AGG 0.681967 2.4:-32953067 MS.gene77090:CDS
CAATATCCTCCTATCCAAGG+AGG 0.696180 2.4:-32952829 MS.gene77090:CDS
TTCATTCTGGAAGAGCTGGG+AGG 0.705305 2.4:-32954574 MS.gene77090:CDS
AGACCTATAAAAGCCGAAGG+CGG 0.722914 2.4:-32953006 MS.gene77090:CDS
AGGTTGCTTCAGTCATATGG+GGG 0.727655 2.4:-32954554 MS.gene77090:intron
TTATCGGGAGTATCAGACGG+TGG 0.745082 2.4:-32952715 MS.gene77090:CDS
CAGAAGATAAGACCTCACCG+GGG 0.863209 2.4:+32953055 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCTTTATAATATTAATAATC+AGG + chr2.4:32953719-32953738 None:intergenic 15.0%
!! TTACTGTTTGATATTAATAT+AGG + chr2.4:32954490-32954509 None:intergenic 15.0%
!! TTTAATATTCAAAACCATAT+TGG - chr2.4:32953772-32953791 MS.gene77090:intron 15.0%
!!! AAAAAAAAAGTCATTTTGAA+AGG + chr2.4:32953741-32953760 None:intergenic 15.0%
!!! AAACAAAAAGATTCATTTTA+TGG - chr2.4:32953324-32953343 MS.gene77090:intron 15.0%
!! AAGAGAAAATTTGTAACAAA+TGG + chr2.4:32953147-32953166 None:intergenic 20.0%
!! AGTTGAAAATTAGAGTATAA+TGG - chr2.4:32952760-32952779 MS.gene77090:CDS 20.0%
!! ATTAAGAACAAAGTGAAAAT+TGG + chr2.4:32952876-32952895 None:intergenic 20.0%
!! TTAAGAACAAAGTGAAAATT+GGG + chr2.4:32952875-32952894 None:intergenic 20.0%
!! TTAATAATCAGGAAAGTAAA+TGG + chr2.4:32953708-32953727 None:intergenic 20.0%
! AAACTGAATTACGAATACTT+TGG + chr2.4:32952982-32953001 None:intergenic 25.0%
! AAATATCGAGCTTGAAATTT+GGG - chr2.4:32952655-32952674 MS.gene77090:CDS 25.0%
! AAATCCACTAAAAAGTAGTT+AGG + chr2.4:32952519-32952538 None:intergenic 25.0%
! AAATTATACTAGAGAGTTTG+AGG - chr2.4:32953536-32953555 MS.gene77090:intron 25.0%
! AATTGTTTATTGTTGTACGA+AGG + chr2.4:32954079-32954098 None:intergenic 25.0%
! AGATAATGATATCATCTACT+TGG - chr2.4:32952809-32952828 MS.gene77090:CDS 25.0%
! ATAATTGGAATAGAAGAAAG+AGG + chr2.4:32954025-32954044 None:intergenic 25.0%
! ATTCAAAACCATATTGGTAA+AGG - chr2.4:32953778-32953797 MS.gene77090:intron 25.0%
! TAAATATCGAGCTTGAAATT+TGG - chr2.4:32952654-32952673 MS.gene77090:CDS 25.0%
! TATGAAAACCTTTACCAATA+TGG + chr2.4:32953789-32953808 None:intergenic 25.0%
!! TTGAATTCTGATTGATTCTA+TGG - chr2.4:32953092-32953111 MS.gene77090:CDS 25.0%
!! TTTACAATTTTCCACAAAGA+TGG - chr2.4:32952940-32952959 MS.gene77090:CDS 25.0%
AAACATGACTCATGCTATAA+TGG + chr2.4:32954402-32954421 None:intergenic 30.0%
ACAAAAGTGGGAGAATAATT+CGG + chr2.4:32953176-32953195 None:intergenic 30.0%
AGAAATGTGGAAATAGTAAC+TGG - chr2.4:32953960-32953979 MS.gene77090:intron 30.0%
AGATGACAAATGATAAAGGA+TGG + chr2.4:32953209-32953228 None:intergenic 30.0%
ATTGTTGAACAAGTCACTAA+TGG + chr2.4:32953285-32953304 None:intergenic 30.0%
ATTTGGATTCTTTAGTCACA+TGG - chr2.4:32953249-32953268 MS.gene77090:intron 30.0%
CACTAAAAAGTAGTTAGGAA+GGG + chr2.4:32952514-32952533 None:intergenic 30.0%
CCAAAGATGACAAATGATAA+AGG + chr2.4:32953213-32953232 None:intergenic 30.0%
CCTTTATCATTTGTCATCTT+TGG - chr2.4:32953210-32953229 MS.gene77090:intron 30.0%
GAGTAATTAACAAGATTGTG+TGG - chr2.4:32953847-32953866 MS.gene77090:intron 30.0%
GATGATATCATTATCTGTGA+AGG + chr2.4:32952805-32952824 None:intergenic 30.0%
GGTTTAGTGACTATCATAAT+CGG - chr2.4:32953345-32953364 MS.gene77090:intron 30.0%
TAATTACTCAGAGACAAACA+TGG + chr2.4:32953836-32953855 None:intergenic 30.0%
TCAAATAATCCAATGCAAAC+AGG - chr2.4:32953647-32953666 MS.gene77090:intron 30.0%
TGAGGATGTGCATTTATAAT+TGG + chr2.4:32954040-32954059 None:intergenic 30.0%
TTGGAATAATGATTGTGATC+CGG + chr2.4:32954197-32954216 None:intergenic 30.0%
TTTCCTTTCCTTCAAGAAAA+TGG - chr2.4:32954097-32954116 MS.gene77090:intron 30.0%
! AATGCCAAATTTTCGTCAAT+GGG - chr2.4:32953377-32953396 MS.gene77090:intron 30.0%
! GACTTGTTCAACAATTACAA+TGG - chr2.4:32953290-32953309 MS.gene77090:intron 30.0%
!! AATTATTCTCCCACTTTTGT+TGG - chr2.4:32953176-32953195 MS.gene77090:intron 30.0%
!! GTGTAAAGCTAGCTTTAATT+TGG - chr2.4:32953232-32953251 MS.gene77090:intron 30.0%
AAAAGATGCTGATGTTAGCA+TGG + chr2.4:32953900-32953919 None:intergenic 35.0%
AACATCTGATCTTGATCCAA+TGG + chr2.4:32952592-32952611 None:intergenic 35.0%
AATAACTAAATGACCAGGCA+TGG + chr2.4:32954365-32954384 None:intergenic 35.0%
ATAACTAAATGACCAGGCAT+GGG + chr2.4:32954364-32954383 None:intergenic 35.0%
ATCAGCTGTTCAAGGATTAT+CGG - chr2.4:32954261-32954280 MS.gene77090:intron 35.0%
ATGGATATAGATCTACAGCA+TGG - chr2.4:32953111-32953130 MS.gene77090:CDS 35.0%
ATTGAAGTCTTCACTTCCTA+AGG + chr2.4:32954227-32954246 None:intergenic 35.0%
ATTGTTGTACGAAGGAAACA+GGG + chr2.4:32954071-32954090 None:intergenic 35.0%
CATATGGGGGTAATATTCTA+TGG - chr2.4:32952451-32952470 MS.gene77090:CDS 35.0%
CATGCAATTAATTAGCCCAT+TGG - chr2.4:32952563-32952582 MS.gene77090:CDS 35.0%
CCACTAAAAAGTAGTTAGGA+AGG + chr2.4:32952515-32952534 None:intergenic 35.0%
CTTACAGATAGTAGATTCCA+AGG - chr2.4:32953872-32953891 MS.gene77090:intron 35.0%
TAAAAAGTAGTTAGGAAGGG+TGG + chr2.4:32952511-32952530 None:intergenic 35.0%
TAATTAGCTGTTCGTCCAAA+TGG + chr2.4:32953405-32953424 None:intergenic 35.0%
TATTGTTGTACGAAGGAAAC+AGG + chr2.4:32954072-32954091 None:intergenic 35.0%
TCCGTATGATTTAACTCAGA+CGG + chr2.4:32954451-32954470 None:intergenic 35.0%
TCCGTCTGAGTTAAATCATA+CGG - chr2.4:32954447-32954466 MS.gene77090:intron 35.0%
TCTGAGTTAAATCATACGGA+TGG - chr2.4:32954451-32954470 MS.gene77090:intron 35.0%
TTATTCCAAGATACCTCCTT+AGG - chr2.4:32954208-32954227 MS.gene77090:intron 35.0%
! ATGAGTCATGTTTCTGAAAG+TGG - chr2.4:32954409-32954428 MS.gene77090:intron 35.0%
! ATGTAGCACCATTTTCTTGA+AGG + chr2.4:32954108-32954127 None:intergenic 35.0%
! GAATGCCAAATTTTCGTCAA+TGG - chr2.4:32953376-32953395 MS.gene77090:intron 35.0%
! GTCTTATCTTCTGAGAAATG+TGG - chr2.4:32953947-32953966 MS.gene77090:intron 35.0%
!! CAATGGGCTAATTAATTGCA+TGG + chr2.4:32952565-32952584 None:intergenic 35.0%
!!! ACATCTTTTTTCCCTTCATC+GGG + chr2.4:32953064-32953083 None:intergenic 35.0%
!!! CACATCTTTTTTCCCTTCAT+CGG + chr2.4:32953065-32953084 None:intergenic 35.0%
!!! CCTTCCTAACTACTTTTTAG+TGG - chr2.4:32952512-32952531 MS.gene77090:CDS 35.0%
AAGATGGAATGCCTATCATC+TGG - chr2.4:32952956-32952975 MS.gene77090:CDS 40.0%
AATTCTTCAAGCCAATCGAC+AGG - chr2.4:32954334-32954353 MS.gene77090:intron 40.0%
ACTATCCAGGTTTCATTTGC+TGG - chr2.4:32952314-32952333 MS.gene77090:CDS 40.0%
AGAAGAAAGAGGCCTAAAAC+CGG + chr2.4:32954014-32954033 None:intergenic 40.0%
AGACTGCTTGGAATAGCTTA+TGG + chr2.4:32953600-32953619 None:intergenic 40.0%
AGTCAATATCCTCCTATCCA+AGG - chr2.4:32954160-32954179 MS.gene77090:intron 40.0%
ATGATTCTTCCTGCTCTCTA+CGG - chr2.4:32954680-32954699 MS.gene77090:CDS 40.0%
CCAGAATGAATACCAACTTG+TGG + chr2.4:32952408-32952427 None:intergenic 40.0%
CTCGGAATAACTAAATGACC+AGG + chr2.4:32954370-32954389 None:intergenic 40.0%
CTTCAGCAAATATATGCACG+TGG + chr2.4:32952742-32952761 None:intergenic 40.0%
GAAACAGAATCAGCTGTTCA+AGG - chr2.4:32954253-32954272 MS.gene77090:intron 40.0%
GAATACTTTGGCCAGATGAT+AGG + chr2.4:32952970-32952989 None:intergenic 40.0%
GACTCATGCTATAATGGCTA+TGG + chr2.4:32954396-32954415 None:intergenic 40.0%
GCAATTAATTAGCCCATTGG+TGG - chr2.4:32952566-32952585 MS.gene77090:CDS 40.0%
GGTGCAAAACCAACAAAAGT+GGG + chr2.4:32953188-32953207 None:intergenic 40.0%
GGTTCCCATTGACGAAAATT+TGG + chr2.4:32953384-32953403 None:intergenic 40.0%
TCAGCTGTTCAAGGATTATC+GGG - chr2.4:32954262-32954281 MS.gene77090:intron 40.0%
TGAAATCGCCCATTTCGAAA+TGG - chr2.4:32954510-32954529 MS.gene77090:intron 40.0%
TGATAGGCATTCCATCTTTG+TGG + chr2.4:32952954-32952973 None:intergenic 40.0%
TGGATTCTTTAGTCACATGG+TGG - chr2.4:32953252-32953271 MS.gene77090:intron 40.0%
TGGTGCAAAACCAACAAAAG+TGG + chr2.4:32953189-32953208 None:intergenic 40.0%
TTTCGTCAATGGGAACCATT+TGG - chr2.4:32953387-32953406 MS.gene77090:intron 40.0%
! GCACCATTTTCTTGAAGGAA+AGG + chr2.4:32954103-32954122 None:intergenic 40.0%
! GTATTCATTCTGGAAGAGCT+GGG - chr2.4:32952415-32952434 MS.gene77090:CDS 40.0%
! TTGCTGGCTGAATTTGATGA+TGG - chr2.4:32952330-32952349 MS.gene77090:CDS 40.0%
!! CCACAAGTTGGTATTCATTC+TGG - chr2.4:32952405-32952424 MS.gene77090:CDS 40.0%
!! CGATTGGCTTGAAGAATTCT+GGG + chr2.4:32954332-32954351 None:intergenic 40.0%
!! TCGATTGGCTTGAAGAATTC+TGG + chr2.4:32954333-32954352 None:intergenic 40.0%
!!! CATCTTTTTTCCCTTCATCG+GGG + chr2.4:32953063-32953082 None:intergenic 40.0%
AGACCTATAAAAGCCGAAGG+CGG - chr2.4:32953986-32954005 MS.gene77090:intron 45.0%
AGGTTGCTTCAGTCATATGG+GGG - chr2.4:32952438-32952457 MS.gene77090:CDS 45.0%
ATATGCCAAGAGATACTCCC+CGG - chr2.4:32953920-32953939 MS.gene77090:intron 45.0%
CAATATCCTCCTATCCAAGG+AGG - chr2.4:32954163-32954182 MS.gene77090:intron 45.0%
CCATTTCGAAATGGCAGATG+GGG - chr2.4:32954519-32954538 MS.gene77090:intron 45.0%
CCCATTTCGAAATGGCAGAT+GGG - chr2.4:32954518-32954537 MS.gene77090:intron 45.0%
CGACAGTTGAAGCAAGTCAA+TGG + chr2.4:32954605-32954624 None:intergenic 45.0%
CTATAATGGCTATGGCTGCT+CGG + chr2.4:32954388-32954407 None:intergenic 45.0%
CTCAGAAGATAAGACCTCAC+CGG + chr2.4:32953942-32953961 None:intergenic 45.0%
CTGATGTTAGCATGGTTCCT+TGG + chr2.4:32953892-32953911 None:intergenic 45.0%
GAAGTCTTCACTTCCTAAGG+AGG + chr2.4:32954224-32954243 None:intergenic 45.0%
GAGGTTGCTTCAGTCATATG+GGG - chr2.4:32952437-32952456 MS.gene77090:CDS 45.0%
GCATTCCGCGATCAATACAT+AGG + chr2.4:32952706-32952725 None:intergenic 45.0%
GCTTTACAGCCTGTTTGCAT+TGG + chr2.4:32953659-32953678 None:intergenic 45.0%
GCTTTCTACGACGCTCATTA+TGG + chr2.4:32952386-32952405 None:intergenic 45.0%
GGAAACAGGGTTCTTGAATG+AGG + chr2.4:32954058-32954077 None:intergenic 45.0%
GGAAGGGTGGAAAATTGAGA+AGG + chr2.4:32952498-32952517 None:intergenic 45.0%
GGAGGTTGCTTCAGTCATAT+GGG - chr2.4:32952436-32952455 MS.gene77090:CDS 45.0%
TCAGAAGATAAGACCTCACC+GGG + chr2.4:32953941-32953960 None:intergenic 45.0%
TGCAGACCTATAAAAGCCGA+AGG - chr2.4:32953983-32954002 MS.gene77090:intron 45.0%
TGCCATCTTCACAGGAAAGA+CGG + chr2.4:32954579-32954598 None:intergenic 45.0%
TTACGTAATCCGTAGAGAGC+AGG + chr2.4:32954692-32954711 None:intergenic 45.0%
TTAGAACCTGTGAGACTGCT+TGG + chr2.4:32953612-32953631 None:intergenic 45.0%
TTCAGCCAGCAAATGAAACC+TGG + chr2.4:32952322-32952341 None:intergenic 45.0%
TTCCGCGATCAATACATAGG+TGG + chr2.4:32952703-32952722 None:intergenic 45.0%
TTGATCCAATGGCCACCAAT+GGG + chr2.4:32952581-32952600 None:intergenic 45.0%
! ATTTTCTCGCTTCACCTGCA+AGG + chr2.4:32954662-32954681 None:intergenic 45.0%
! CCCATCTGCCATTTCGAAAT+GGG + chr2.4:32954521-32954540 None:intergenic 45.0%
! GACCACCTATGTATTGATCG+CGG - chr2.4:32952698-32952717 MS.gene77090:CDS 45.0%
! GGTATTCATTCTGGAAGAGC+TGG - chr2.4:32952414-32952433 MS.gene77090:CDS 45.0%
! TGTTTCTGAAAGTGGCGTCT+CGG - chr2.4:32954417-32954436 MS.gene77090:intron 45.0%
!! AATGGTGCTACATCTGCTAC+TGG - chr2.4:32954115-32954134 MS.gene77090:intron 45.0%
!! CACTTCCTAAGGAGGTATCT+TGG + chr2.4:32954216-32954235 None:intergenic 45.0%
!! GGATTATCGGGAGTATCAGA+CGG - chr2.4:32954274-32954293 MS.gene77090:intron 45.0%
!! TTTGAATATTAAATAAAAAA+TGG + chr2.4:32953765-32953784 None:intergenic 5.0%
AATGGTCGTGCCATCTTCAC+AGG + chr2.4:32954587-32954606 None:intergenic 50.0%
ACGTGTTCTTACCCCGATGA+AGG - chr2.4:32953049-32953068 MS.gene77090:CDS 50.0%
AGCTGTCGTAAACGTCTTGC+AGG - chr2.4:32952360-32952379 MS.gene77090:CDS 50.0%
ATAAAAGCCGAAGGCGGAAC+CGG - chr2.4:32953992-32954011 MS.gene77090:intron 50.0%
ATCCAAGGAGGCTCGAATTC+CGG - chr2.4:32954175-32954194 MS.gene77090:intron 50.0%
CAGAAGATAAGACCTCACCG+GGG + chr2.4:32953940-32953959 None:intergenic 50.0%
CGTCGTAGAAAGCCACAAGT+TGG - chr2.4:32952393-32952412 MS.gene77090:CDS 50.0%
CGTGTTCTTACCCCGATGAA+GGG - chr2.4:32953050-32953069 MS.gene77090:CDS 50.0%
CTTGATCCAATGGCCACCAA+TGG + chr2.4:32952582-32952601 None:intergenic 50.0%
GAATTCGAGCCTCCTTGGAT+AGG + chr2.4:32954175-32954194 None:intergenic 50.0%
GACCGTCTTTCCTGTGAAGA+TGG - chr2.4:32954574-32954593 MS.gene77090:CDS 50.0%
GCATGGGAATTCCTGTCGAT+TGG + chr2.4:32954348-32954367 None:intergenic 50.0%
GCCCATTTCGAAATGGCAGA+TGG - chr2.4:32954517-32954536 MS.gene77090:intron 50.0%
GCTATTCCAAGCAGTCTCAC+AGG - chr2.4:32953603-32953622 MS.gene77090:intron 50.0%
GGGAGGTTGCTTCAGTCATA+TGG - chr2.4:32952435-32952454 MS.gene77090:CDS 50.0%
TTCAGCGTGTTGAGCATCAG+AGG - chr2.4:32954632-32954651 MS.gene77090:CDS 50.0%
! AATGGCAGATGGGGTTTTCC+AGG - chr2.4:32954528-32954547 MS.gene77090:intron 50.0%
! ATGGCAGATGGGGTTTTCCA+GGG - chr2.4:32954529-32954548 MS.gene77090:intron 50.0%
! ATTAGCCCATTGGTGGCCAT+TGG - chr2.4:32952573-32952592 MS.gene77090:CDS 50.0%
! CCCCATCTGCCATTTCGAAA+TGG + chr2.4:32954522-32954541 None:intergenic 50.0%
! GTATTGATCGCGGAATGCGT+GGG - chr2.4:32952708-32952727 MS.gene77090:CDS 50.0%
! GTTCCGCCTTCGGCTTTTAT+AGG + chr2.4:32953992-32954011 None:intergenic 50.0%
! TGTATTGATCGCGGAATGCG+TGG - chr2.4:32952707-32952726 MS.gene77090:CDS 50.0%
! TTCATTCTGGAAGAGCTGGG+AGG - chr2.4:32952418-32952437 MS.gene77090:CDS 50.0%
!! TTATCGGGAGTATCAGACGG+TGG - chr2.4:32954277-32954296 MS.gene77090:intron 50.0%
ATCCGGAATTCGAGCCTCCT+TGG + chr2.4:32954180-32954199 None:intergenic 55.0%
CGTTGACAAAATCCGACCCC+TGG + chr2.4:32954549-32954568 None:intergenic 55.0%
CTGAAAGTGGCGTCTCGGAT+AGG - chr2.4:32954422-32954441 MS.gene77090:intron 55.0%
TCGACAGGAATTCCCATGCC+TGG - chr2.4:32954349-32954368 MS.gene77090:intron 55.0%
TTCGAGCCTCCTTGGATAGG+AGG + chr2.4:32954172-32954191 None:intergenic 55.0%
! AGATGGGGTTTTCCAGGGGT+CGG - chr2.4:32954534-32954553 MS.gene77090:intron 55.0%
! CCTAAAACCGGTTCCGCCTT+CGG + chr2.4:32954002-32954021 None:intergenic 55.0%
! TGGCAGATGGGGTTTTCCAG+GGG - chr2.4:32954530-32954549 MS.gene77090:intron 55.0%
CCAAGAGATACTCCCCGGTG+AGG - chr2.4:32953925-32953944 MS.gene77090:intron 60.0%
!! CCGAAGGCGGAACCGGTTTT+AGG - chr2.4:32953999-32954018 MS.gene77090:intron 60.0%
!! CCTCACCGGGGAGTATCTCT+TGG + chr2.4:32953928-32953947 None:intergenic 60.0%
GCATCAGAGGCGAGCCTTGC+AGG - chr2.4:32954645-32954664 MS.gene77090:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32952303 32954711 32952303 ID=MS.gene77090
chr2.4 mRNA 32952303 32954711 32952303 ID=MS.gene77090.t1;Parent=MS.gene77090
chr2.4 exon 32954555 32954711 32954555 ID=MS.gene77090.t1.exon1;Parent=MS.gene77090.t1
chr2.4 CDS 32954555 32954711 32954555 ID=cds.MS.gene77090.t1;Parent=MS.gene77090.t1
chr2.4 exon 32952303 32953135 32952303 ID=MS.gene77090.t1.exon2;Parent=MS.gene77090.t1
chr2.4 CDS 32952303 32953135 32952303 ID=cds.MS.gene77090.t1;Parent=MS.gene77090.t1
Gene Sequence

>MS.gene77090

ATGTTTAGCTTACTATCCAGGTTTCATTTGCTGGCTGAATTTGATGATGGTAAGCGTAGCTGTCGTAAACGTCTTGCAGGCCATAATGAGCGTCGTAGAAAGCCACAAGTTGGTATTCATTCTGGAAGAGCTGGGAGGTTGCTTCAGTCATATGGGGGTAATATTCTATGGAAAATAATTTAATCTTTTGTTCCTTCTCAATTTTCCACCCTTCCTAACTACTTTTTAGTGGATTTCTTGCATTTACAAGTTAACGCATCCATGCAATTAATTAGCCCATTGGTGGCCATTGGATCAAGATCAGATGTTGCAGAAAGCAAGCAAATTTTGATTTTTTTTTTAAAGAACACTTAAATATCGAGCTTGAAATTTGGGCAGTCTGATCTTGATCTAACGACCACCTATGTATTGATCGCGGAATGCGTGGGATTTGTGACCACGTGCATATATTTGCTGAAGTTGAAAATTAGAGTATAATGGATAACAATAATTTTAAAATCCTTCACAGATAATGATATCATCTACTTGGTTTTGATGCGTATATAGAAGCATGCTATAGTAAAATTGTTCCCAATTTTCACTTTGTTCTTAATTTCTCAATTTTGATATTGATTATTGTATTCTGTTGTACATTTGCTTTACAATTTTCCACAAAGATGGAATGCCTATCATCTGGCCAAAGTATTCGTAATTCAGTTTCATCGATAAAATTGTGTTCTTGAAGCGATTTTCATAAAAGAATAAGCACGTGTTCTTACCCCGATGAAGGGAAAAAAGATGTGATACTTTTTGAATTCTGATTGATTCTATGGATATAGATCTACAGCATGGTGATTATGTGCCATTTGTTACAAATTTTCTCTTTTGTTTCCGAATTATTCTCCCACTTTTGTTGGTTTTGCACCATCCTTTATCATTTGTCATCTTTGGTGTAAAGCTAGCTTTAATTTGGATTCTTTAGTCACATGGTGGATATGAACCATTAGTGACTTGTTCAACAATTACAATGGAATCATATCTGAAACAAAAAGATTCATTTTATGGTTTAGTGACTATCATAATCGGTAATCTTAGAATGCCAAATTTTCGTCAATGGGAACCATTTGGACGAACAGCTAATTAAGCACTTCTAGCATAAGCTCTTATGACATAAGTACGTATGTATAAGCTATTTCTAAAAGAAAAGATAAAAAAAGTCAATTTCTTTTCGTATAAGCTATAAGTTGATTTTAGAAATTATACTAGAGAGTTTGAGGAAAAAATTGAAAACATATTGATTTGTCATAAGTTGTTTCCATAAGCTATTCCAAGCAGTCTCACAGGTTCTAATGTTAGTAGATAAGCTCAAATAATCCAATGCAAACAGGCTGTAAAGCTATCTCTGCTTACGAACGATAATTATCCATTTACTTTCCTGATTATTAATATTATAAAGCCTTTCAAAATGACTTTTTTTTTACCATTTTTTATTTAATATTCAAAACCATATTGGTAAAGGTTTTCATAATATATATTTTCTGTTGTTGTAATCCATGTTTGTCTCTGAGTAATTAACAAGATTGTGTGGTACTTACAGATAGTAGATTCCAAGGAACCATGCTAACATCAGCATCTTTTATATGCCAAGAGATACTCCCCGGTGAGGTCTTATCTTCTGAGAAATGTGGAAATAGTAACTGGTGCAGACCTATAAAAGCCGAAGGCGGAACCGGTTTTAGGCCTCTTTCTTCTATTCCAATTATAAATGCACATCCTCATTCAAGAACCCTGTTTCCTTCGTACAACAATAAACAATTTCCTTTCCTTCAAGAAAATGGTGCTACATCTGCTACTGGAAGCATCTTCTGTGAAAACAGTAGTCAATATCCTCCTATCCAAGGAGGCTCGAATTCCGGATCACAATCATTATTCCAAGATACCTCCTTAGGAAGTGAAGACTTCAATGTTTTTGAAACAGAATCAGCTGTTCAAGGATTATCGGGAGTATCAGACGGTGGTTGTGCTCTCTCTCTTCTGTCATCTCAATCCCAGAATTCTTCAAGCCAATCGACAGGAATTCCCATGCCTGGTCATTTAGTTATTCCGAGCAGCCATAGCCATTATAGCATGAGTCATGTTTCTGAAAGTGGCGTCTCGGATAGGTTTCCGTCTGAGTTAAATCATACGGATGGAAGTCATCTTAGTCCTATATTAATATCAAACAGTAATGAAATCGCCCATTTCGAAATGGCAGATGGGGTTTTCCAGGGGTCGGATTTTGTCAACGTGAAAGACCGTCTTTCCTGTGAAGATGGCACGACCATTGACTTGCTTCAACTGTCGTCACAACTTCAGCGTGTTGAGCATCAGAGGCGAGCCTTGCAGGTGAAGCGAGAAAATGATTCTTCCTGCTCTCTACGGATTACGTAA
Protein sequence

>MS.gene77090.t1

MFSLLSRFHLLAEFDDGKRSCRKRLAGHNERRRKPQVGIHSGRAGRLLQSYGDSRFQGTMLTSASFICQEILPGEVLSSEKCGNSNWCRPIKAEGGTGFRPLSSIPIINAHPHSRTLFPSYNNKQFPFLQENGATSATGSIFCENSSQYPPIQGGSNSGSQSLFQDTSLGSEDFNVFETESAVQGLSGVSDGGCALSLLSSQSQNSSSQSTGIPMPGHLVIPSSHSHYSMSHVSESGVSDRFPSELNHTDGSHLSPILISNSNEIAHFEMADGVFQGSDFVNVKDRLSCEDGTTIDLLQLSSQLQRVEHQRRALQVKRENDSSCSLRIT