AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77091

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77091.t1 MTR_2g461920 96.914 324 10 0 6 329 216 539 0.0 643
MS.gene77091.t1 MTR_4g109770 62.691 327 95 6 6 329 168 470 3.77e-121 357
MS.gene77091.t1 MTR_5g046670 42.029 138 70 3 6 142 289 417 8.95e-18 84.3
MS.gene77091.t1 MTR_7g028740 32.057 209 92 7 6 206 122 288 4.52e-11 63.5
MS.gene77091.t1 MTR_1g053715 87.097 31 4 0 6 36 119 149 5.51e-11 63.2
MS.gene77091.t1 MTR_7g092930 60.345 58 21 2 6 61 113 170 6.36e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77091.t1 AT1G69170 51.389 72 34 1 6 77 169 239 4.74e-15 75.9
MS.gene77091.t1 AT5G50670 38.095 147 73 7 6 144 146 282 8.18e-13 68.9
MS.gene77091.t1 AT5G50670 38.095 147 73 7 6 144 146 282 8.18e-13 68.9
MS.gene77091.t1 AT5G50570 38.095 147 73 7 6 144 146 282 8.18e-13 68.9
MS.gene77091.t1 AT5G50570 38.095 147 73 7 6 144 146 282 8.18e-13 68.9
MS.gene77091.t1 AT2G42200 54.839 62 22 2 6 61 119 180 9.06e-11 62.8

Find 89 sgRNAs with CRISPR-Local

Find 171 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCCTTTCCTTCAAGAAAA+TGG 0.189331 2.4:-32886039 MS.gene77091:CDS
TCGATTGGCTTGAAGAATTC+TGG 0.190530 2.4:+32885806 None:intergenic
TGAGGATGTGCATTTATAAT+TGG 0.243639 2.4:+32886099 None:intergenic
CCACAAGTTGGTATTCATTC+TGG 0.255651 2.4:-32887731 MS.gene77091:CDS
ACTATCCAGGTTTCATTTGC+TGG 0.261393 2.4:-32887822 MS.gene77091:CDS
GGGAGGTTGCTTCAGTCATA+TGG 0.315390 2.4:-32887701 MS.gene77091:CDS
GCATCAGAGGCGAGCCTTGC+AGG 0.317553 2.4:-32885491 MS.gene77091:CDS
AAACATGACTCATGCTATAA+TGG 0.320615 2.4:+32885737 None:intergenic
TTACTGTTTGATATTAATAT+AGG 0.332199 2.4:+32885649 None:intergenic
TATTGTTGTACGAAGGAAAC+AGG 0.337560 2.4:+32886067 None:intergenic
ATCCAAGGAGGCTCGAATTC+CGG 0.339302 2.4:-32885961 MS.gene77091:CDS
GCTTTCTACGACGCTCATTA+TGG 0.341005 2.4:+32887753 None:intergenic
CGATTGGCTTGAAGAATTCT+GGG 0.354670 2.4:+32885807 None:intergenic
GGAGGTTGCTTCAGTCATAT+GGG 0.364253 2.4:-32887700 MS.gene77091:CDS
CCCCATCTGCCATTTCGAAA+TGG 0.368112 2.4:+32885617 None:intergenic
CACTTCCTAAGGAGGTATCT+TGG 0.394888 2.4:+32885923 None:intergenic
CCCATCTGCCATTTCGAAAT+GGG 0.406149 2.4:+32885618 None:intergenic
AGCTGTCGTAAACGTCTTGC+AGG 0.408574 2.4:-32887776 MS.gene77091:CDS
CCTAAAACCGGTTCCGCCTT+CGG 0.416837 2.4:+32886137 None:intergenic
ATAAAAGCCGAAGGCGGAAC+CGG 0.423047 2.4:-32886144 MS.gene77091:CDS
ATCCGGAATTCGAGCCTCCT+TGG 0.439031 2.4:+32885959 None:intergenic
TAATGTTTAGCTTACTATCC+AGG 0.439138 2.4:-32887835 MS.gene77091:CDS
ATCAGCTGTTCAAGGATTAT+CGG 0.450349 2.4:-32885875 MS.gene77091:CDS
GGTATTCATTCTGGAAGAGC+TGG 0.460050 2.4:-32887722 MS.gene77091:CDS
TTATTCCAAGATACCTCCTT+AGG 0.463781 2.4:-32885928 MS.gene77091:CDS
ATTGAAGTCTTCACTTCCTA+AGG 0.476688 2.4:+32885912 None:intergenic
GAAACAGAATCAGCTGTTCA+AGG 0.477684 2.4:-32885883 MS.gene77091:CDS
AGAAATGTGGAAATAGTAAC+TGG 0.480981 2.4:-32886176 MS.gene77091:CDS
AATGGTCGTGCCATCTTCAC+AGG 0.486457 2.4:+32885552 None:intergenic
ATGAGTCATGTTTCTGAAAG+TGG 0.489233 2.4:-32885727 MS.gene77091:CDS
GACCGTCTTTCCTGTGAAGA+TGG 0.490853 2.4:-32885562 MS.gene77091:CDS
CTGAAAGTGGCGTCTCGGAT+AGG 0.491794 2.4:-32885714 MS.gene77091:CDS
TGAAATCGCCCATTTCGAAA+TGG 0.493806 2.4:-32885626 MS.gene77091:CDS
TCAGCTGTTCAAGGATTATC+GGG 0.494261 2.4:-32885874 MS.gene77091:CDS
GCATGGGAATTCCTGTCGAT+TGG 0.495766 2.4:+32885791 None:intergenic
CTGATGTTAGCATGGTTCCT+TGG 0.502694 2.4:+32886247 None:intergenic
CCTCACCGGGGAGTATCTCT+TGG 0.503402 2.4:+32886211 None:intergenic
AATGGTGCTACATCTGCTAC+TGG 0.508879 2.4:-32886021 MS.gene77091:CDS
ATAATTGGAATAGAAGAAAG+AGG 0.509513 2.4:+32886114 None:intergenic
ATGATTCTTCCTGCTCTCTA+CGG 0.510890 2.4:-32885456 MS.gene77091:CDS
TCCGTCTGAGTTAAATCATA+CGG 0.511466 2.4:-32885689 MS.gene77091:CDS
CCCATTTCGAAATGGCAGAT+GGG 0.511484 2.4:-32885618 MS.gene77091:CDS
TTCAGCCAGCAAATGAAACC+TGG 0.518515 2.4:+32887817 None:intergenic
TTACGTAATCCGTAGAGAGC+AGG 0.520716 2.4:+32885447 None:intergenic
TTGCTGGCTGAATTTGATGA+TGG 0.522089 2.4:-32887806 MS.gene77091:CDS
AGAAGAAAGAGGCCTAAAAC+CGG 0.523177 2.4:+32886125 None:intergenic
TTGGAATAATGATTGTGATC+CGG 0.528558 2.4:+32885942 None:intergenic
CTATAATGGCTATGGCTGCT+CGG 0.529046 2.4:+32885751 None:intergenic
CGACAGTTGAAGCAAGTCAA+TGG 0.529505 2.4:+32885534 None:intergenic
AATAACTAAATGACCAGGCA+TGG 0.532105 2.4:+32885774 None:intergenic
GCCCATTTCGAAATGGCAGA+TGG 0.532890 2.4:-32885619 MS.gene77091:CDS
TCGACAGGAATTCCCATGCC+TGG 0.536060 2.4:-32885787 MS.gene77091:CDS
CCATTTCGAAATGGCAGATG+GGG 0.536154 2.4:-32885617 MS.gene77091:CDS
TCAGAAGATAAGACCTCACC+GGG 0.542752 2.4:+32886198 None:intergenic
GAGGTTGCTTCAGTCATATG+GGG 0.543950 2.4:-32887699 MS.gene77091:CDS
TTCGAGCCTCCTTGGATAGG+AGG 0.548698 2.4:+32885967 None:intergenic
GACTCATGCTATAATGGCTA+TGG 0.554681 2.4:+32885743 None:intergenic
CGTTGACAAAATCCGACCCC+TGG 0.557627 2.4:+32885590 None:intergenic
GTATTCATTCTGGAAGAGCT+GGG 0.561734 2.4:-32887721 MS.gene77091:CDS
GAATTCGAGCCTCCTTGGAT+AGG 0.563220 2.4:+32885964 None:intergenic
TGTTTCTGAAAGTGGCGTCT+CGG 0.565635 2.4:-32885719 MS.gene77091:CDS
AATTGTTTATTGTTGTACGA+AGG 0.567910 2.4:+32886060 None:intergenic
TCCGTATGATTTAACTCAGA+CGG 0.569364 2.4:+32885688 None:intergenic
AATTCTTCAAGCCAATCGAC+AGG 0.569495 2.4:-32885802 MS.gene77091:CDS
GAAGTCTTCACTTCCTAAGG+AGG 0.571303 2.4:+32885915 None:intergenic
CTTACAGATAGTAGATTCCA+AGG 0.572899 2.4:-32886264 MS.gene77091:intron
CTCAGAAGATAAGACCTCAC+CGG 0.577587 2.4:+32886197 None:intergenic
ATATGCCAAGAGATACTCCC+CGG 0.578163 2.4:-32886216 MS.gene77091:CDS
CTCGGAATAACTAAATGACC+AGG 0.578215 2.4:+32885769 None:intergenic
GGAAACAGGGTTCTTGAATG+AGG 0.578570 2.4:+32886081 None:intergenic
TGCCATCTTCACAGGAAAGA+CGG 0.587669 2.4:+32885560 None:intergenic
AGTCAATATCCTCCTATCCA+AGG 0.597117 2.4:-32885976 MS.gene77091:CDS
AAAAGATGCTGATGTTAGCA+TGG 0.597201 2.4:+32886239 None:intergenic
TTCAGCGTGTTGAGCATCAG+AGG 0.601537 2.4:-32885504 MS.gene77091:CDS
CGTCGTAGAAAGCCACAAGT+TGG 0.607871 2.4:-32887743 MS.gene77091:CDS
CCAGAATGAATACCAACTTG+TGG 0.611115 2.4:+32887731 None:intergenic
TCTGAGTTAAATCATACGGA+TGG 0.611214 2.4:-32885685 MS.gene77091:CDS
ATAACTAAATGACCAGGCAT+GGG 0.613150 2.4:+32885775 None:intergenic
GTCTTATCTTCTGAGAAATG+TGG 0.623862 2.4:-32886189 MS.gene77091:CDS
TGCAGACCTATAAAAGCCGA+AGG 0.654610 2.4:-32886153 MS.gene77091:CDS
GGATTATCGGGAGTATCAGA+CGG 0.658305 2.4:-32885862 MS.gene77091:CDS
ATTGTTGTACGAAGGAAACA+GGG 0.661617 2.4:+32886068 None:intergenic
CCAAGAGATACTCCCCGGTG+AGG 0.681967 2.4:-32886211 MS.gene77091:CDS
CAATATCCTCCTATCCAAGG+AGG 0.696180 2.4:-32885973 MS.gene77091:CDS
TTCATTCTGGAAGAGCTGGG+AGG 0.705305 2.4:-32887718 MS.gene77091:CDS
AGACCTATAAAAGCCGAAGG+CGG 0.722914 2.4:-32886150 MS.gene77091:CDS
AGGTTGCTTCAGTCATATGG+GGG 0.727655 2.4:-32887698 MS.gene77091:intron
TTATCGGGAGTATCAGACGG+TGG 0.745082 2.4:-32885859 MS.gene77091:CDS
CAGAAGATAAGACCTCACCG+GGG 0.863209 2.4:+32886199 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GCTTTATAATATTAATAATC+AGG + chr2.4:32886863-32886882 None:intergenic 15.0%
!! TTACTGTTTGATATTAATAT+AGG + chr2.4:32887634-32887653 None:intergenic 15.0%
!! TTTAATATTCAAAACCATAT+TGG - chr2.4:32886916-32886935 MS.gene77091:intron 15.0%
!!! AAAAAAAAAGTCATTTTGAA+AGG + chr2.4:32886885-32886904 None:intergenic 15.0%
!!! AAACAAAAAGATTCATTTTA+TGG - chr2.4:32886468-32886487 MS.gene77091:intron 15.0%
!! AAGAGAAAATTTGTAACAAA+TGG + chr2.4:32886291-32886310 None:intergenic 20.0%
!! AGTTGAAAATTAGAGTATAA+TGG - chr2.4:32885904-32885923 MS.gene77091:CDS 20.0%
!! ATTAAGAACAAAGTGAAAAT+TGG + chr2.4:32886020-32886039 None:intergenic 20.0%
!! TTAAGAACAAAGTGAAAATT+GGG + chr2.4:32886019-32886038 None:intergenic 20.0%
!! TTAATAATCAGGAAAGTAAA+TGG + chr2.4:32886852-32886871 None:intergenic 20.0%
! AAACTGAATTACGAATACTT+TGG + chr2.4:32886126-32886145 None:intergenic 25.0%
! AAATATCGAGCTTGAAATTT+GGG - chr2.4:32885799-32885818 MS.gene77091:CDS 25.0%
! AAATCCACTAAAAAGTAGTT+AGG + chr2.4:32885663-32885682 None:intergenic 25.0%
! AAATTATACTAGAGAGTTTG+AGG - chr2.4:32886680-32886699 MS.gene77091:intron 25.0%
! AATTGTTTATTGTTGTACGA+AGG + chr2.4:32887223-32887242 None:intergenic 25.0%
! AGATAATGATATCATCTACT+TGG - chr2.4:32885953-32885972 MS.gene77091:CDS 25.0%
! ATAATTGGAATAGAAGAAAG+AGG + chr2.4:32887169-32887188 None:intergenic 25.0%
! ATTCAAAACCATATTGGTAA+AGG - chr2.4:32886922-32886941 MS.gene77091:intron 25.0%
! TAAATATCGAGCTTGAAATT+TGG - chr2.4:32885798-32885817 MS.gene77091:CDS 25.0%
! TATGAAAACCTTTACCAATA+TGG + chr2.4:32886933-32886952 None:intergenic 25.0%
!! TTGAATTCTGATTGATTCTA+TGG - chr2.4:32886236-32886255 MS.gene77091:CDS 25.0%
!! TTTACAATTTTCCACAAAGA+TGG - chr2.4:32886084-32886103 MS.gene77091:CDS 25.0%
AAACATGACTCATGCTATAA+TGG + chr2.4:32887546-32887565 None:intergenic 30.0%
ACAAAAGTGGGAGAATAATT+CGG + chr2.4:32886320-32886339 None:intergenic 30.0%
AGAAATGTGGAAATAGTAAC+TGG - chr2.4:32887104-32887123 MS.gene77091:intron 30.0%
AGATGACAAATGATAAAGGA+TGG + chr2.4:32886353-32886372 None:intergenic 30.0%
ATTGTTGAACAAGTCACTAA+TGG + chr2.4:32886429-32886448 None:intergenic 30.0%
ATTTGGATTCTTTAGTCACA+TGG - chr2.4:32886393-32886412 MS.gene77091:intron 30.0%
CACTAAAAAGTAGTTAGGAA+GGG + chr2.4:32885658-32885677 None:intergenic 30.0%
CCAAAGATGACAAATGATAA+AGG + chr2.4:32886357-32886376 None:intergenic 30.0%
CCTTTATCATTTGTCATCTT+TGG - chr2.4:32886354-32886373 MS.gene77091:intron 30.0%
GAGTAATTAACAAGATTGTG+TGG - chr2.4:32886991-32887010 MS.gene77091:intron 30.0%
GATGATATCATTATCTGTGA+AGG + chr2.4:32885949-32885968 None:intergenic 30.0%
GGTTTAGTGACTATCATAAT+CGG - chr2.4:32886489-32886508 MS.gene77091:intron 30.0%
TAATTACTCAGAGACAAACA+TGG + chr2.4:32886980-32886999 None:intergenic 30.0%
TCAAATAATCCAATGCAAAC+AGG - chr2.4:32886791-32886810 MS.gene77091:intron 30.0%
TGAGGATGTGCATTTATAAT+TGG + chr2.4:32887184-32887203 None:intergenic 30.0%
TTGGAATAATGATTGTGATC+CGG + chr2.4:32887341-32887360 None:intergenic 30.0%
TTTCCTTTCCTTCAAGAAAA+TGG - chr2.4:32887241-32887260 MS.gene77091:intron 30.0%
! AATGCCAAATTTTCGTCAAT+GGG - chr2.4:32886521-32886540 MS.gene77091:intron 30.0%
! GACTTGTTCAACAATTACAA+TGG - chr2.4:32886434-32886453 MS.gene77091:intron 30.0%
!! AATTATTCTCCCACTTTTGT+TGG - chr2.4:32886320-32886339 MS.gene77091:intron 30.0%
!! GTGTAAAGCTAGCTTTAATT+TGG - chr2.4:32886376-32886395 MS.gene77091:intron 30.0%
AAAAGATGCTGATGTTAGCA+TGG + chr2.4:32887044-32887063 None:intergenic 35.0%
AACATCTGATCTTGATCCAA+TGG + chr2.4:32885736-32885755 None:intergenic 35.0%
AATAACTAAATGACCAGGCA+TGG + chr2.4:32887509-32887528 None:intergenic 35.0%
ATAACTAAATGACCAGGCAT+GGG + chr2.4:32887508-32887527 None:intergenic 35.0%
ATCAGCTGTTCAAGGATTAT+CGG - chr2.4:32887405-32887424 MS.gene77091:intron 35.0%
ATGGATATAGATCTACAGCA+TGG - chr2.4:32886255-32886274 MS.gene77091:CDS 35.0%
ATTGAAGTCTTCACTTCCTA+AGG + chr2.4:32887371-32887390 None:intergenic 35.0%
ATTGTTGTACGAAGGAAACA+GGG + chr2.4:32887215-32887234 None:intergenic 35.0%
CATATGGGGGTAATATTCTA+TGG - chr2.4:32885595-32885614 MS.gene77091:CDS 35.0%
CATGCAATTAATTAGCCCAT+TGG - chr2.4:32885707-32885726 MS.gene77091:CDS 35.0%
CCACTAAAAAGTAGTTAGGA+AGG + chr2.4:32885659-32885678 None:intergenic 35.0%
CTTACAGATAGTAGATTCCA+AGG - chr2.4:32887016-32887035 MS.gene77091:intron 35.0%
TAAAAAGTAGTTAGGAAGGG+TGG + chr2.4:32885655-32885674 None:intergenic 35.0%
TAATTAGCTGTTCGTCCAAA+TGG + chr2.4:32886549-32886568 None:intergenic 35.0%
TATTGTTGTACGAAGGAAAC+AGG + chr2.4:32887216-32887235 None:intergenic 35.0%
TCCGTATGATTTAACTCAGA+CGG + chr2.4:32887595-32887614 None:intergenic 35.0%
TCCGTCTGAGTTAAATCATA+CGG - chr2.4:32887591-32887610 MS.gene77091:intron 35.0%
TCTGAGTTAAATCATACGGA+TGG - chr2.4:32887595-32887614 MS.gene77091:intron 35.0%
TTATTCCAAGATACCTCCTT+AGG - chr2.4:32887352-32887371 MS.gene77091:intron 35.0%
! ATGAGTCATGTTTCTGAAAG+TGG - chr2.4:32887553-32887572 MS.gene77091:intron 35.0%
! ATGTAGCACCATTTTCTTGA+AGG + chr2.4:32887252-32887271 None:intergenic 35.0%
! GAATGCCAAATTTTCGTCAA+TGG - chr2.4:32886520-32886539 MS.gene77091:intron 35.0%
! GTCTTATCTTCTGAGAAATG+TGG - chr2.4:32887091-32887110 MS.gene77091:intron 35.0%
!! CAATGGGCTAATTAATTGCA+TGG + chr2.4:32885709-32885728 None:intergenic 35.0%
!!! ACATCTTTTTTCCCTTCATC+GGG + chr2.4:32886208-32886227 None:intergenic 35.0%
!!! CACATCTTTTTTCCCTTCAT+CGG + chr2.4:32886209-32886228 None:intergenic 35.0%
!!! CCTTCCTAACTACTTTTTAG+TGG - chr2.4:32885656-32885675 MS.gene77091:CDS 35.0%
AAGATGGAATGCCTATCATC+TGG - chr2.4:32886100-32886119 MS.gene77091:CDS 40.0%
AATTCTTCAAGCCAATCGAC+AGG - chr2.4:32887478-32887497 MS.gene77091:intron 40.0%
ACTATCCAGGTTTCATTTGC+TGG - chr2.4:32885458-32885477 MS.gene77091:CDS 40.0%
AGAAGAAAGAGGCCTAAAAC+CGG + chr2.4:32887158-32887177 None:intergenic 40.0%
AGACTGCTTGGAATAGCTTA+TGG + chr2.4:32886744-32886763 None:intergenic 40.0%
AGTCAATATCCTCCTATCCA+AGG - chr2.4:32887304-32887323 MS.gene77091:intron 40.0%
ATGATTCTTCCTGCTCTCTA+CGG - chr2.4:32887824-32887843 MS.gene77091:CDS 40.0%
CCAGAATGAATACCAACTTG+TGG + chr2.4:32885552-32885571 None:intergenic 40.0%
CTCGGAATAACTAAATGACC+AGG + chr2.4:32887514-32887533 None:intergenic 40.0%
CTTCAGCAAATATATGCACG+TGG + chr2.4:32885886-32885905 None:intergenic 40.0%
GAAACAGAATCAGCTGTTCA+AGG - chr2.4:32887397-32887416 MS.gene77091:intron 40.0%
GAATACTTTGGCCAGATGAT+AGG + chr2.4:32886114-32886133 None:intergenic 40.0%
GACTCATGCTATAATGGCTA+TGG + chr2.4:32887540-32887559 None:intergenic 40.0%
GCAATTAATTAGCCCATTGG+TGG - chr2.4:32885710-32885729 MS.gene77091:CDS 40.0%
GGTGCAAAACCAACAAAAGT+GGG + chr2.4:32886332-32886351 None:intergenic 40.0%
GGTTCCCATTGACGAAAATT+TGG + chr2.4:32886528-32886547 None:intergenic 40.0%
TCAGCTGTTCAAGGATTATC+GGG - chr2.4:32887406-32887425 MS.gene77091:intron 40.0%
TGAAATCGCCCATTTCGAAA+TGG - chr2.4:32887654-32887673 MS.gene77091:intron 40.0%
TGATAGGCATTCCATCTTTG+TGG + chr2.4:32886098-32886117 None:intergenic 40.0%
TGGATTCTTTAGTCACATGG+TGG - chr2.4:32886396-32886415 MS.gene77091:intron 40.0%
TGGTGCAAAACCAACAAAAG+TGG + chr2.4:32886333-32886352 None:intergenic 40.0%
TTTCGTCAATGGGAACCATT+TGG - chr2.4:32886531-32886550 MS.gene77091:intron 40.0%
! GCACCATTTTCTTGAAGGAA+AGG + chr2.4:32887247-32887266 None:intergenic 40.0%
! GTATTCATTCTGGAAGAGCT+GGG - chr2.4:32885559-32885578 MS.gene77091:CDS 40.0%
! TTGCTGGCTGAATTTGATGA+TGG - chr2.4:32885474-32885493 MS.gene77091:CDS 40.0%
!! CCACAAGTTGGTATTCATTC+TGG - chr2.4:32885549-32885568 MS.gene77091:CDS 40.0%
!! CGATTGGCTTGAAGAATTCT+GGG + chr2.4:32887476-32887495 None:intergenic 40.0%
!! TCGATTGGCTTGAAGAATTC+TGG + chr2.4:32887477-32887496 None:intergenic 40.0%
!!! CATCTTTTTTCCCTTCATCG+GGG + chr2.4:32886207-32886226 None:intergenic 40.0%
AGACCTATAAAAGCCGAAGG+CGG - chr2.4:32887130-32887149 MS.gene77091:intron 45.0%
AGGTTGCTTCAGTCATATGG+GGG - chr2.4:32885582-32885601 MS.gene77091:CDS 45.0%
ATATGCCAAGAGATACTCCC+CGG - chr2.4:32887064-32887083 MS.gene77091:intron 45.0%
CAATATCCTCCTATCCAAGG+AGG - chr2.4:32887307-32887326 MS.gene77091:intron 45.0%
CCATTTCGAAATGGCAGATG+GGG - chr2.4:32887663-32887682 MS.gene77091:intron 45.0%
CCCATTTCGAAATGGCAGAT+GGG - chr2.4:32887662-32887681 MS.gene77091:intron 45.0%
CGACAGTTGAAGCAAGTCAA+TGG + chr2.4:32887749-32887768 None:intergenic 45.0%
CTATAATGGCTATGGCTGCT+CGG + chr2.4:32887532-32887551 None:intergenic 45.0%
CTCAGAAGATAAGACCTCAC+CGG + chr2.4:32887086-32887105 None:intergenic 45.0%
CTGATGTTAGCATGGTTCCT+TGG + chr2.4:32887036-32887055 None:intergenic 45.0%
GAAGTCTTCACTTCCTAAGG+AGG + chr2.4:32887368-32887387 None:intergenic 45.0%
GAGGTTGCTTCAGTCATATG+GGG - chr2.4:32885581-32885600 MS.gene77091:CDS 45.0%
GCATTCCGCGATCAATACAT+AGG + chr2.4:32885850-32885869 None:intergenic 45.0%
GCTTTACAGCCTGTTTGCAT+TGG + chr2.4:32886803-32886822 None:intergenic 45.0%
GCTTTCTACGACGCTCATTA+TGG + chr2.4:32885530-32885549 None:intergenic 45.0%
GGAAACAGGGTTCTTGAATG+AGG + chr2.4:32887202-32887221 None:intergenic 45.0%
GGAAGGGTGGAAAATTGAGA+AGG + chr2.4:32885642-32885661 None:intergenic 45.0%
GGAGGTTGCTTCAGTCATAT+GGG - chr2.4:32885580-32885599 MS.gene77091:CDS 45.0%
TCAGAAGATAAGACCTCACC+GGG + chr2.4:32887085-32887104 None:intergenic 45.0%
TGCAGACCTATAAAAGCCGA+AGG - chr2.4:32887127-32887146 MS.gene77091:intron 45.0%
TGCCATCTTCACAGGAAAGA+CGG + chr2.4:32887723-32887742 None:intergenic 45.0%
TTACGTAATCCGTAGAGAGC+AGG + chr2.4:32887836-32887855 None:intergenic 45.0%
TTAGAACCTGTGAGACTGCT+TGG + chr2.4:32886756-32886775 None:intergenic 45.0%
TTCAGCCAGCAAATGAAACC+TGG + chr2.4:32885466-32885485 None:intergenic 45.0%
TTCCGCGATCAATACATAGG+TGG + chr2.4:32885847-32885866 None:intergenic 45.0%
TTGATCCAATGGCCACCAAT+GGG + chr2.4:32885725-32885744 None:intergenic 45.0%
! ATTTTCTCGCTTCACCTGCA+AGG + chr2.4:32887806-32887825 None:intergenic 45.0%
! CCCATCTGCCATTTCGAAAT+GGG + chr2.4:32887665-32887684 None:intergenic 45.0%
! GACCACCTATGTATTGATCG+CGG - chr2.4:32885842-32885861 MS.gene77091:CDS 45.0%
! GGTATTCATTCTGGAAGAGC+TGG - chr2.4:32885558-32885577 MS.gene77091:CDS 45.0%
! TGTTTCTGAAAGTGGCGTCT+CGG - chr2.4:32887561-32887580 MS.gene77091:intron 45.0%
!! AATGGTGCTACATCTGCTAC+TGG - chr2.4:32887259-32887278 MS.gene77091:intron 45.0%
!! CACTTCCTAAGGAGGTATCT+TGG + chr2.4:32887360-32887379 None:intergenic 45.0%
!! GGATTATCGGGAGTATCAGA+CGG - chr2.4:32887418-32887437 MS.gene77091:intron 45.0%
!! TTTGAATATTAAATAAAAAA+TGG + chr2.4:32886909-32886928 None:intergenic 5.0%
AATGGTCGTGCCATCTTCAC+AGG + chr2.4:32887731-32887750 None:intergenic 50.0%
ACGTGTTCTTACCCCGATGA+AGG - chr2.4:32886193-32886212 MS.gene77091:CDS 50.0%
AGCTGTCGTAAACGTCTTGC+AGG - chr2.4:32885504-32885523 MS.gene77091:CDS 50.0%
ATAAAAGCCGAAGGCGGAAC+CGG - chr2.4:32887136-32887155 MS.gene77091:intron 50.0%
ATCCAAGGAGGCTCGAATTC+CGG - chr2.4:32887319-32887338 MS.gene77091:intron 50.0%
CAGAAGATAAGACCTCACCG+GGG + chr2.4:32887084-32887103 None:intergenic 50.0%
CGTCGTAGAAAGCCACAAGT+TGG - chr2.4:32885537-32885556 MS.gene77091:CDS 50.0%
CGTGTTCTTACCCCGATGAA+GGG - chr2.4:32886194-32886213 MS.gene77091:CDS 50.0%
CTTGATCCAATGGCCACCAA+TGG + chr2.4:32885726-32885745 None:intergenic 50.0%
GAATTCGAGCCTCCTTGGAT+AGG + chr2.4:32887319-32887338 None:intergenic 50.0%
GACCGTCTTTCCTGTGAAGA+TGG - chr2.4:32887718-32887737 MS.gene77091:CDS 50.0%
GCATGGGAATTCCTGTCGAT+TGG + chr2.4:32887492-32887511 None:intergenic 50.0%
GCCCATTTCGAAATGGCAGA+TGG - chr2.4:32887661-32887680 MS.gene77091:intron 50.0%
GCTATTCCAAGCAGTCTCAC+AGG - chr2.4:32886747-32886766 MS.gene77091:intron 50.0%
GGGAGGTTGCTTCAGTCATA+TGG - chr2.4:32885579-32885598 MS.gene77091:CDS 50.0%
TTCAGCGTGTTGAGCATCAG+AGG - chr2.4:32887776-32887795 MS.gene77091:CDS 50.0%
! AATGGCAGATGGGGTTTTCC+AGG - chr2.4:32887672-32887691 MS.gene77091:intron 50.0%
! ATGGCAGATGGGGTTTTCCA+GGG - chr2.4:32887673-32887692 MS.gene77091:intron 50.0%
! ATTAGCCCATTGGTGGCCAT+TGG - chr2.4:32885717-32885736 MS.gene77091:CDS 50.0%
! CCCCATCTGCCATTTCGAAA+TGG + chr2.4:32887666-32887685 None:intergenic 50.0%
! GTATTGATCGCGGAATGCGT+GGG - chr2.4:32885852-32885871 MS.gene77091:CDS 50.0%
! GTTCCGCCTTCGGCTTTTAT+AGG + chr2.4:32887136-32887155 None:intergenic 50.0%
! TGTATTGATCGCGGAATGCG+TGG - chr2.4:32885851-32885870 MS.gene77091:CDS 50.0%
! TTCATTCTGGAAGAGCTGGG+AGG - chr2.4:32885562-32885581 MS.gene77091:CDS 50.0%
!! TTATCGGGAGTATCAGACGG+TGG - chr2.4:32887421-32887440 MS.gene77091:intron 50.0%
ATCCGGAATTCGAGCCTCCT+TGG + chr2.4:32887324-32887343 None:intergenic 55.0%
CGTTGACAAAATCCGACCCC+TGG + chr2.4:32887693-32887712 None:intergenic 55.0%
CTGAAAGTGGCGTCTCGGAT+AGG - chr2.4:32887566-32887585 MS.gene77091:intron 55.0%
TCGACAGGAATTCCCATGCC+TGG - chr2.4:32887493-32887512 MS.gene77091:intron 55.0%
TTCGAGCCTCCTTGGATAGG+AGG + chr2.4:32887316-32887335 None:intergenic 55.0%
! AGATGGGGTTTTCCAGGGGT+CGG - chr2.4:32887678-32887697 MS.gene77091:intron 55.0%
! CCTAAAACCGGTTCCGCCTT+CGG + chr2.4:32887146-32887165 None:intergenic 55.0%
! TGGCAGATGGGGTTTTCCAG+GGG - chr2.4:32887674-32887693 MS.gene77091:intron 55.0%
CCAAGAGATACTCCCCGGTG+AGG - chr2.4:32887069-32887088 MS.gene77091:intron 60.0%
!! CCGAAGGCGGAACCGGTTTT+AGG - chr2.4:32887143-32887162 MS.gene77091:intron 60.0%
!! CCTCACCGGGGAGTATCTCT+TGG + chr2.4:32887072-32887091 None:intergenic 60.0%
GCATCAGAGGCGAGCCTTGC+AGG - chr2.4:32887789-32887808 MS.gene77091:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32885447 32887855 32885447 ID=MS.gene77091
chr2.4 mRNA 32885447 32887855 32885447 ID=MS.gene77091.t1;Parent=MS.gene77091
chr2.4 exon 32887699 32887855 32887699 ID=MS.gene77091.t1.exon1;Parent=MS.gene77091.t1
chr2.4 CDS 32887699 32887855 32887699 ID=cds.MS.gene77091.t1;Parent=MS.gene77091.t1
chr2.4 exon 32885447 32886279 32885447 ID=MS.gene77091.t1.exon2;Parent=MS.gene77091.t1
chr2.4 CDS 32885447 32886279 32885447 ID=cds.MS.gene77091.t1;Parent=MS.gene77091.t1
Gene Sequence

>MS.gene77091

ATGTTTAGCTTACTATCCAGGTTTCATTTGCTGGCTGAATTTGATGATGGTAAGCGTAGCTGTCGTAAACGTCTTGCAGGCCATAATGAGCGTCGTAGAAAGCCACAAGTTGGTATTCATTCTGGAAGAGCTGGGAGGTTGCTTCAGTCATATGGGGGTAATATTCTATGGAAAATAATTTAATCTTTTGTTCCTTCTCAATTTTCCACCCTTCCTAACTACTTTTTAGTGGATTTCTTGCATTTACAAGTTAACGCATCCATGCAATTAATTAGCCCATTGGTGGCCATTGGATCAAGATCAGATGTTGCAGAAAGCAAGCAAATTTTGATTTTTTTTTTAAAGAACACTTAAATATCGAGCTTGAAATTTGGGCAGTCTGATCTTGATCTAACGACCACCTATGTATTGATCGCGGAATGCGTGGGATTTGTGACCACGTGCATATATTTGCTGAAGTTGAAAATTAGAGTATAATGGATAACAATAATTTTAAAATCCTTCACAGATAATGATATCATCTACTTGGTTTTGATGCGTATATAGAAGCATGCTATAGTAAAATTGTTCCCAATTTTCACTTTGTTCTTAATTTCTCAATTTTGATATTGATTATTGTATTCTGTTGTACATTTGCTTTACAATTTTCCACAAAGATGGAATGCCTATCATCTGGCCAAAGTATTCGTAATTCAGTTTCATCGATAAAATTGTGTTCTTGAAGCGATTTTCATAAAAGAATAAGCACGTGTTCTTACCCCGATGAAGGGAAAAAAGATGTGATACTTTTTGAATTCTGATTGATTCTATGGATATAGATCTACAGCATGGTGATTATGTGCCATTTGTTACAAATTTTCTCTTTTGTTTCCGAATTATTCTCCCACTTTTGTTGGTTTTGCACCATCCTTTATCATTTGTCATCTTTGGTGTAAAGCTAGCTTTAATTTGGATTCTTTAGTCACATGGTGGATATGAACCATTAGTGACTTGTTCAACAATTACAATGGAATCATATCTGAAACAAAAAGATTCATTTTATGGTTTAGTGACTATCATAATCGGTAATCTTAGAATGCCAAATTTTCGTCAATGGGAACCATTTGGACGAACAGCTAATTAAGCACTTCTAGCATAAGCTCTTATGACATAAGTACGTATGTATAAGCTATTTCTAAAAGAAAAGATAAAAAAAGTCAATTTCTTTTCGTATAAGCTATAAGTTGATTTTAGAAATTATACTAGAGAGTTTGAGGAAAAAATTGAAAACATATTGATTTGTCATAAGTTGTTTCCATAAGCTATTCCAAGCAGTCTCACAGGTTCTAATGTTAGTAGATAAGCTCAAATAATCCAATGCAAACAGGCTGTAAAGCTATCTCTGCTTACGAACGATAATTATCCATTTACTTTCCTGATTATTAATATTATAAAGCCTTTCAAAATGACTTTTTTTTTACCATTTTTTATTTAATATTCAAAACCATATTGGTAAAGGTTTTCATAATATATATTTTCTGTTGTTGTAATCCATGTTTGTCTCTGAGTAATTAACAAGATTGTGTGGTACTTACAGATAGTAGATTCCAAGGAACCATGCTAACATCAGCATCTTTTATATGCCAAGAGATACTCCCCGGTGAGGTCTTATCTTCTGAGAAATGTGGAAATAGTAACTGGTGCAGACCTATAAAAGCCGAAGGCGGAACCGGTTTTAGGCCTCTTTCTTCTATTCCAATTATAAATGCACATCCTCATTCAAGAACCCTGTTTCCTTCGTACAACAATAAACAATTTCCTTTCCTTCAAGAAAATGGTGCTACATCTGCTACTGGAAGCATCTTCTGTGAAAACAGTAGTCAATATCCTCCTATCCAAGGAGGCTCGAATTCCGGATCACAATCATTATTCCAAGATACCTCCTTAGGAAGTGAAGACTTCAATGTTTTTGAAACAGAATCAGCTGTTCAAGGATTATCGGGAGTATCAGACGGTGGTTGTGCTCTCTCTCTTCTGTCATCTCAATCCCAGAATTCTTCAAGCCAATCGACAGGAATTCCCATGCCTGGTCATTTAGTTATTCCGAGCAGCCATAGCCATTATAGCATGAGTCATGTTTCTGAAAGTGGCGTCTCGGATAGGTTTCCGTCTGAGTTAAATCATACGGATGGAAGTCATCTTAGTCCTATATTAATATCAAACAGTAATGAAATCGCCCATTTCGAAATGGCAGATGGGGTTTTCCAGGGGTCGGATTTTGTCAACGTGAAAGACCGTCTTTCCTGTGAAGATGGCACGACCATTGACTTGCTTCAACTGTCGTCACAACTTCAGCGTGTTGAGCATCAGAGGCGAGCCTTGCAGGTGAAGCGAGAAAATGATTCTTCCTGCTCTCTACGGATTACGTAA
Protein sequence

>MS.gene77091.t1

MFSLLSRFHLLAEFDDGKRSCRKRLAGHNERRRKPQVGIHSGRAGRLLQSYGDSRFQGTMLTSASFICQEILPGEVLSSEKCGNSNWCRPIKAEGGTGFRPLSSIPIINAHPHSRTLFPSYNNKQFPFLQENGATSATGSIFCENSSQYPPIQGGSNSGSQSLFQDTSLGSEDFNVFETESAVQGLSGVSDGGCALSLLSSQSQNSSSQSTGIPMPGHLVIPSSHSHYSMSHVSESGVSDRFPSELNHTDGSHLSPILISNSNEIAHFEMADGVFQGSDFVNVKDRLSCEDGTTIDLLQLSSQLQRVEHQRRALQVKRENDSSCSLRIT