AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77097

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77097.t1 MTR_2g462000 98.153 379 7 0 1 379 64 442 0.0 786
MS.gene77097.t1 MTR_6g015880 70.787 356 100 1 1 356 59 410 0.0 566
MS.gene77097.t1 MTR_7g015960 67.925 371 112 2 1 371 49 412 0.0 561
MS.gene77097.t1 MTR_7g015960 67.925 371 112 2 1 371 59 422 0.0 560
MS.gene77097.t1 MTR_2g462010 61.640 378 137 3 3 377 76 448 0.0 516
MS.gene77097.t1 MTR_7g015950 60.052 383 142 4 2 378 68 445 5.93e-180 507
MS.gene77097.t1 MTR_6g015875 59.309 376 144 4 2 373 69 439 1.88e-173 491
MS.gene77097.t1 MTR_6g015865 57.703 357 136 4 3 355 13 358 9.78e-158 447
MS.gene77097.t1 MTR_2g104890 49.072 377 181 4 3 372 70 442 3.00e-150 432
MS.gene77097.t1 MTR_3g117690 39.683 378 206 8 3 370 71 436 1.34e-103 313
MS.gene77097.t1 MTR_2g034950 28.910 422 242 20 5 379 243 653 2.19e-31 125
MS.gene77097.t1 MTR_4g121080 27.937 383 230 16 5 344 239 618 5.08e-31 124
MS.gene77097.t1 MTR_5g005590 28.831 385 219 17 5 344 244 618 1.78e-30 123
MS.gene77097.t1 MTR_2g083210 27.225 382 229 17 5 345 260 633 5.34e-26 110
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77097.t1 AT5G15630 73.315 356 91 2 2 357 25 376 0.0 575
MS.gene77097.t1 AT5G15630 73.315 356 91 2 2 357 61 412 0.0 575
MS.gene77097.t1 AT5G15630 73.315 356 91 2 2 357 12 363 0.0 575
MS.gene77097.t1 AT5G15630 73.315 356 91 2 2 357 12 363 0.0 575
MS.gene77097.t1 AT5G60920 62.943 367 130 3 2 364 77 441 0.0 519
MS.gene77097.t1 AT5G60920 62.943 367 130 3 2 364 30 394 0.0 516
MS.gene77097.t1 AT3G02210 58.621 377 150 3 3 376 75 448 5.09e-174 493
MS.gene77097.t1 AT3G29810 58.133 375 145 4 3 372 70 437 1.16e-172 489
MS.gene77097.t1 AT5G15630 69.718 284 82 2 2 285 61 340 1.61e-153 436
MS.gene77097.t1 AT1G09790 49.718 354 168 5 3 353 66 412 2.11e-124 366
MS.gene77097.t1 AT1G09790 49.718 354 168 5 3 353 77 423 2.84e-124 366
MS.gene77097.t1 AT5G60950 48.980 147 69 1 3 149 62 202 1.34e-43 150
MS.gene77097.t1 AT5G49270 28.756 386 225 17 5 344 250 631 2.43e-35 137
MS.gene77097.t1 AT4G16120 28.238 386 225 18 5 344 251 630 2.58e-34 134
MS.gene77097.t1 AT4G27110 27.446 419 252 17 5 377 253 665 1.94e-30 123
MS.gene77097.t1 AT3G20580 29.257 417 237 20 5 375 263 667 8.51e-30 121
MS.gene77097.t1 AT3G16860 27.725 422 242 23 5 377 244 651 2.67e-28 117

Find 98 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCCATATGATTTCTTTCT+TGG 0.151399 2.4:+32784463 None:intergenic
CCTAGAAGGGTATACTTTAA+TGG 0.184050 2.4:-32782352 MS.gene77097:CDS
TATTTCCAGCAACCTCTTTC+AGG 0.198489 2.4:+32783174 None:intergenic
AAGATAATTATAAGGATTAT+TGG 0.226624 2.4:-32783064 MS.gene77097:CDS
AACACAACAGCTTGGGTTCT+TGG 0.235897 2.4:+32783656 None:intergenic
GGTAGCCTTTGAGCAGAATT+TGG 0.247873 2.4:+32782286 None:intergenic
CCATTAAAGTATACCCTTCT+AGG 0.259971 2.4:+32782352 None:intergenic
AACTGTCTTGTTAGTCCTTC+CGG 0.267626 2.4:+32784226 None:intergenic
TGGCGTGTGCATTGCAATAA+AGG 0.319433 2.4:+32783119 None:intergenic
ATTCTACAATGATTTAATAA+TGG 0.323384 2.4:-32782461 MS.gene77097:CDS
GCATATAAAAGATAATTATA+AGG 0.335357 2.4:-32783072 MS.gene77097:CDS
GTGCATTGCAATAAAGGTTT+TGG 0.357973 2.4:+32783125 None:intergenic
GGGTAACATTACTGAGATTA+GGG 0.358595 2.4:+32782970 None:intergenic
TAATGGAAGCTGGGGCTAAA+GGG 0.368160 2.4:-32782444 MS.gene77097:CDS
GATGACACAGGAATGTTCTA+TGG 0.385600 2.4:-32782490 MS.gene77097:CDS
TGTTGCTTGCGCTCCCATCA+TGG 0.386890 2.4:+32784442 None:intergenic
ATGAACCCGGGATGGACATT+AGG 0.394711 2.4:-32784497 MS.gene77097:CDS
AGGTGGTGTGCTAACATCTT+GGG 0.396771 2.4:-32784300 MS.gene77097:CDS
TGGGTAACATTACTGAGATT+AGG 0.404033 2.4:+32782969 None:intergenic
CAAGTAGTTGACTTATTACC+TGG 0.416180 2.4:-32784359 MS.gene77097:CDS
GGGCCAAGAAAGAAATCATA+TGG 0.421488 2.4:-32784466 MS.gene77097:CDS
TGAGCAGAATTTGGCAAGAA+AGG 0.426117 2.4:+32782295 None:intergenic
TAGAATGAATTACACACAAT+GGG 0.426364 2.4:-32783009 MS.gene77097:CDS
ATATGCCAATGCACACGAAC+AGG 0.426620 2.4:+32783089 None:intergenic
ATAATGGAAGCTGGGGCTAA+AGG 0.427586 2.4:-32782445 MS.gene77097:CDS
TCTTGGGGACAGGACCCTTC+AGG 0.429022 2.4:-32784284 MS.gene77097:CDS
AACTTACTTATGGCTTCATA+AGG 0.429069 2.4:+32782915 None:intergenic
GGCAAGAAAGGGTATGAATC+AGG 0.435645 2.4:+32782307 None:intergenic
AATGATTTAATAATGGAAGC+TGG 0.446216 2.4:-32782454 MS.gene77097:CDS
TCTGTAAGAATAACAGTTGA+AGG 0.452263 2.4:+32784134 None:intergenic
AAGATCCTGAAAGAGGTTGC+TGG 0.460769 2.4:-32783179 MS.gene77097:CDS
AAATTGCTTGGACCGGGAGC+AGG 0.463331 2.4:-32784185 MS.gene77097:CDS
GAGCAGAATTTGGCAAGAAA+GGG 0.471958 2.4:+32782296 None:intergenic
AGAAATCATATGGGCCATGA+TGG 0.480849 2.4:-32784456 MS.gene77097:CDS
ATGATTTAATAATGGAAGCT+GGG 0.480905 2.4:-32782453 MS.gene77097:CDS
ATAAAGAAACACAACAGCTT+GGG 0.481011 2.4:+32783649 None:intergenic
GTCCATCCTAATGTCCATCC+CGG 0.482281 2.4:+32784491 None:intergenic
TTTGTCCTGTTCGTGTGCAT+TGG 0.488987 2.4:-32783094 MS.gene77097:CDS
TGCAATCTCGGCATTTCAGA+TGG 0.490026 2.4:-32784261 MS.gene77097:CDS
GGACTTCTCTTGCAACTATG+AGG 0.491344 2.4:+32784383 None:intergenic
ATAAGTACATGTCACATTCC+AGG 0.497470 2.4:+32783695 None:intergenic
GATAAAGAAACACAACAGCT+TGG 0.498328 2.4:+32783648 None:intergenic
AGCAGGATATTCATGTGGTC+CGG 0.500729 2.4:-32784168 MS.gene77097:CDS
GGACCCTTCAGGTGCAATCT+CGG 0.506260 2.4:-32784273 MS.gene77097:CDS
CAAGACAGTTAAACTACCTC+AGG 0.509450 2.4:-32784213 MS.gene77097:CDS
CAGATGGGTGTAGGACTCTC+CGG 0.515620 2.4:-32784245 MS.gene77097:CDS
ATCTGAGGTGCTTATGAAGA+AGG 0.516290 2.4:-32782413 MS.gene77097:CDS
AAGGTGGTGTGCTAACATCT+TGG 0.517435 2.4:-32784301 MS.gene77097:CDS
GTCATCATTACAACACTTGT+AGG 0.519462 2.4:+32782265 None:intergenic
CATTTACACTAAAGCAAGGA+TGG 0.523081 2.4:-32782381 MS.gene77097:CDS
ACAACAGCTTGGGTTCTTGG+AGG 0.528958 2.4:+32783659 None:intergenic
TGTATTCAAAACTATAGACT+TGG 0.537143 2.4:+32782949 None:intergenic
GCTTGTCCAACTTGTGCTTG+TGG 0.548007 2.4:-32783600 MS.gene77097:CDS
GGTAATAAGTCAACTACTTG+AGG 0.550335 2.4:+32784362 None:intergenic
TCTTGCCAAATTCTGCTCAA+AGG 0.554568 2.4:-32782291 MS.gene77097:CDS
AAATGATGCAATAATCACAT+AGG 0.564180 2.4:+32782240 None:intergenic
TGCATATTGTAAGATGCTCC+AGG 0.567353 2.4:+32784341 None:intergenic
GAAATCATATGGGCCATGAT+GGG 0.570678 2.4:-32784455 MS.gene77097:CDS
TGTGCTAACATCTTGGGGAC+AGG 0.571959 2.4:-32784294 MS.gene77097:CDS
TACACAAACTGTTGCAAAGG+TGG 0.573020 2.4:-32784317 MS.gene77097:CDS
ATGCCGAGATTGCACCTGAA+GGG 0.577152 2.4:+32784270 None:intergenic
TTGCTGGAAATAACAAGACT+AGG 0.578763 2.4:-32783163 MS.gene77097:CDS
CCACATGAATATCCTGCTCC+CGG 0.579683 2.4:+32784173 None:intergenic
GTTGAAGGAACTCTCTTAGC+CGG 0.580085 2.4:+32784149 None:intergenic
GGAGCGCAAGCAACAGAACA+AGG 0.580360 2.4:-32784434 MS.gene77097:CDS
CAATACACAAACTGTTGCAA+AGG 0.581528 2.4:-32784320 MS.gene77097:CDS
TCGGCATTTCAGATGGGTGT+AGG 0.583929 2.4:-32784254 MS.gene77097:CDS
TGATTTAATAATGGAAGCTG+GGG 0.584574 2.4:-32782452 MS.gene77097:CDS
TGACAACCACAAGCACAAGT+TGG 0.585659 2.4:+32783594 None:intergenic
ATGGCTTCATAAGGAAGAAG+TGG 0.591100 2.4:+32782924 None:intergenic
TCCATCCTAATGTCCATCCC+GGG 0.595187 2.4:+32784492 None:intergenic
AGATTCAAAGATCCTGAAAG+AGG 0.596172 2.4:-32783186 MS.gene77097:CDS
AAGTTGGACAAGCTGTGACT+TGG 0.597255 2.4:+32783610 None:intergenic
AATGCCGAGATTGCACCTGA+AGG 0.597959 2.4:+32784269 None:intergenic
AAAGGGTATGAATCAGGAGG+TGG 0.602047 2.4:+32782313 None:intergenic
GGCCAAGAAAGAAATCATAT+GGG 0.602816 2.4:-32784465 MS.gene77097:CDS
GATGGACATTAGGATGGACA+TGG 0.605342 2.4:-32784487 MS.gene77097:CDS
GTATTCAAAACTATAGACTT+GGG 0.605359 2.4:+32782950 None:intergenic
AACACATTTACACTAAAGCA+AGG 0.605862 2.4:-32782385 MS.gene77097:CDS
ATACCGAAACATTATGAACC+CGG 0.606089 2.4:-32784510 None:intergenic
GCAATCTCGGCATTTCAGAT+GGG 0.606491 2.4:-32784260 MS.gene77097:CDS
ACCCGGGATGGACATTAGGA+TGG 0.607366 2.4:-32784493 MS.gene77097:CDS
TACCGAAACATTATGAACCC+GGG 0.608883 2.4:-32784509 None:intergenic
ATTTACACTAAAGCAAGGAT+GGG 0.609581 2.4:-32782380 MS.gene77097:CDS
TTATTCTTACAGATGATCGT+AGG 0.609804 2.4:-32784124 MS.gene77097:CDS
GAAACATTATGAACCCGGGA+TGG 0.613102 2.4:-32784505 None:intergenic
GGTGGTGTGCTAACATCTTG+GGG 0.624710 2.4:-32784299 MS.gene77097:CDS
ATAGAATGAATTACACACAA+TGG 0.628977 2.4:-32783010 MS.gene77097:CDS
TAAAGGGAATGTGCAATCTG+AGG 0.631122 2.4:-32782428 MS.gene77097:CDS
CCAAGCAATTTAAAATCCTG+AGG 0.636131 2.4:+32784197 None:intergenic
TGGGTGTAGGACTCTCCGGA+AGG 0.662739 2.4:-32784241 MS.gene77097:CDS
AATGCACACGAACAGGACAA+AGG 0.669891 2.4:+32783096 None:intergenic
CCGGGAGCAGGATATTCATG+TGG 0.685860 2.4:-32784173 MS.gene77097:CDS
GCACACGAACAGGACAAAGG+TGG 0.685930 2.4:+32783099 None:intergenic
AAGAAAGGGTATGAATCAGG+AGG 0.687329 2.4:+32782310 None:intergenic
ATGGACATTAGGATGGACAT+GGG 0.698791 2.4:-32784486 MS.gene77097:CDS
AGAATGAATTACACACAATG+GGG 0.725776 2.4:-32783008 MS.gene77097:CDS
ATTGTGTATGCAGATGACAC+AGG 0.753079 2.4:-32782502 MS.gene77097:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATGTTATAACTTATA+AGG + chr2.4:32783469-32783488 None:intergenic 10.0%
!! TTATAAGTTATAACATTATT+TGG - chr2.4:32783468-32783487 MS.gene77097:intron 10.0%
!! AAGATAATTATAAGGATTAT+TGG - chr2.4:32783658-32783677 MS.gene77097:CDS 15.0%
!! ATTCTACAATGATTTAATAA+TGG - chr2.4:32784261-32784280 MS.gene77097:CDS 15.0%
!! GCATATAAAAGATAATTATA+AGG - chr2.4:32783650-32783669 MS.gene77097:CDS 15.0%
!! ATAGGGATATTATATGTATT+TGG + chr2.4:32784145-32784164 None:intergenic 20.0%
!! ATATTTGAAGAACTTACTTA+TGG + chr2.4:32783820-32783839 None:intergenic 20.0%
!! TAATAGTACATTTGTATTCA+AGG + chr2.4:32782967-32782986 None:intergenic 20.0%
!! TATTAGATAATACTATTTCC+AGG + chr2.4:32782889-32782908 None:intergenic 20.0%
!! TCAAGTAAACAAAATATAGA+CGG + chr2.4:32783849-32783868 None:intergenic 20.0%
!!! AAATAAATTGGCTTGAAATT+TGG + chr2.4:32784014-32784033 None:intergenic 20.0%
!!! AAGACTAATAATCATGATTT+TGG + chr2.4:32783359-32783378 None:intergenic 20.0%
!!! TTATTGCATCATTTTTCATT+TGG - chr2.4:32784491-32784510 MS.gene77097:CDS 20.0%
! AAATGATGCAATAATCACAT+AGG + chr2.4:32784485-32784504 None:intergenic 25.0%
! ACTATTTGCAATATACTCAA+AGG + chr2.4:32782659-32782678 None:intergenic 25.0%
! ATAGAATGAATTACACACAA+TGG - chr2.4:32783712-32783731 MS.gene77097:intron 25.0%
! CTATTTGCAATATACTCAAA+GGG + chr2.4:32782658-32782677 None:intergenic 25.0%
! GAAATTCAGTCAAATACAAT+TGG - chr2.4:32783257-32783276 MS.gene77097:intron 25.0%
! GAGTATATTGCAAATAGTTA+TGG - chr2.4:32782661-32782680 MS.gene77097:intron 25.0%
! GTATTCAAAACTATAGACTT+GGG + chr2.4:32783775-32783794 None:intergenic 25.0%
! GTTGCATCATAATCATTTAT+AGG - chr2.4:32783230-32783249 MS.gene77097:intron 25.0%
! TAATGTGGAAGTAGAAATTA+AGG + chr2.4:32783183-32783202 None:intergenic 25.0%
! TAGAATGAATTACACACAAT+GGG - chr2.4:32783713-32783732 MS.gene77097:intron 25.0%
! TGTATTCAAAACTATAGACT+TGG + chr2.4:32783776-32783795 None:intergenic 25.0%
! TTAAAAGTTGGCTAATAATG+TGG + chr2.4:32783198-32783217 None:intergenic 25.0%
! TTGGATTGTACGATATTAAT+CGG + chr2.4:32783995-32784014 None:intergenic 25.0%
!! AATAATCATGATTTTGGACA+CGG + chr2.4:32783353-32783372 None:intergenic 25.0%
!! AATGATTTAATAATGGAAGC+TGG - chr2.4:32784268-32784287 MS.gene77097:CDS 25.0%
!! ATGATTTAATAATGGAAGCT+GGG - chr2.4:32784269-32784288 MS.gene77097:CDS 25.0%
!!! CACAATATAAAAGGTTTTAG+AGG + chr2.4:32783413-32783432 None:intergenic 25.0%
!!! CTCTAAAACCTTTTATATTG+TGG - chr2.4:32783411-32783430 MS.gene77097:intron 25.0%
!!! GATTATTAGTCTTAACCATA+TGG - chr2.4:32783366-32783385 MS.gene77097:intron 25.0%
!!! GTTTTTTTAACTTTTGTGCA+TGG - chr2.4:32784193-32784212 MS.gene77097:CDS 25.0%
AAAATACCAAGCTTGAAATC+TGG - chr2.4:32784047-32784066 MS.gene77097:intron 30.0%
AACACATTTACACTAAAGCA+AGG - chr2.4:32784337-32784356 MS.gene77097:CDS 30.0%
AACTTACTTATGGCTTCATA+AGG + chr2.4:32783810-32783829 None:intergenic 30.0%
ACTGCATAGGAAAAAAATAC+AGG + chr2.4:32783005-32783024 None:intergenic 30.0%
AGAATGAATTACACACAATG+GGG - chr2.4:32783714-32783733 MS.gene77097:intron 30.0%
ATAAAGAAACACAACAGCTT+GGG + chr2.4:32783076-32783095 None:intergenic 30.0%
ATGTGACTCTATGCATTATA+GGG + chr2.4:32784162-32784181 None:intergenic 30.0%
ATTTACACTAAAGCAAGGAT+GGG - chr2.4:32784342-32784361 MS.gene77097:CDS 30.0%
ATTTGTCATTATGCAAATGC+AGG - chr2.4:32783511-32783530 MS.gene77097:intron 30.0%
CTACATTTGACCATAATTCT+CGG + chr2.4:32782736-32782755 None:intergenic 30.0%
TCTGTAAGAATAACAGTTGA+AGG + chr2.4:32782591-32782610 None:intergenic 30.0%
TGACGTCTCTAATACAAATT+CGG - chr2.4:32783919-32783938 MS.gene77097:intron 30.0%
TTATTCTTACAGATGATCGT+AGG - chr2.4:32782598-32782617 MS.gene77097:intron 30.0%
TTTAATACTGCCGAGAATTA+TGG - chr2.4:32782723-32782742 MS.gene77097:intron 30.0%
! GTTGTCTTATTAAGATCAGA+CGG + chr2.4:32784074-32784093 None:intergenic 30.0%
! TGATTTAATAATGGAAGCTG+GGG - chr2.4:32784270-32784289 MS.gene77097:CDS 30.0%
AGATTCAAAGATCCTGAAAG+AGG - chr2.4:32783536-32783555 MS.gene77097:intron 35.0%
AGGATCTGCAATTACAATCA+AGG + chr2.4:32782821-32782840 None:intergenic 35.0%
ATAAGTACATGTCACATTCC+AGG + chr2.4:32783030-32783049 None:intergenic 35.0%
CAATACACAAACTGTTGCAA+AGG - chr2.4:32782402-32782421 MS.gene77097:CDS 35.0%
CATGTGACTCTATGCATTAT+AGG + chr2.4:32784163-32784182 None:intergenic 35.0%
CATTGCAATCAACATGTTTG+TGG - chr2.4:32783965-32783984 MS.gene77097:intron 35.0%
CATTTACACTAAAGCAAGGA+TGG - chr2.4:32784341-32784360 MS.gene77097:CDS 35.0%
CCATTAAAGTATACCCTTCT+AGG + chr2.4:32784373-32784392 None:intergenic 35.0%
GATAAAGAAACACAACAGCT+TGG + chr2.4:32783077-32783096 None:intergenic 35.0%
GGCCAAGAAAGAAATCATAT+GGG - chr2.4:32782257-32782276 MS.gene77097:CDS 35.0%
GGGTAACATTACTGAGATTA+GGG + chr2.4:32783755-32783774 None:intergenic 35.0%
GGTAATAAGTCAACTACTTG+AGG + chr2.4:32782363-32782382 None:intergenic 35.0%
GTCATCATTACAACACTTGT+AGG + chr2.4:32784460-32784479 None:intergenic 35.0%
TGGGTAACATTACTGAGATT+AGG + chr2.4:32783756-32783775 None:intergenic 35.0%
TTGCTGGAAATAACAAGACT+AGG - chr2.4:32783559-32783578 MS.gene77097:intron 35.0%
! AACCATCAAGGTTTTCGAAA+AGG + chr2.4:32782841-32782860 None:intergenic 35.0%
! ATCCTTTTCGAAAACCTTGA+TGG - chr2.4:32782836-32782855 MS.gene77097:intron 35.0%
! CAAGTAGTTGACTTATTACC+TGG - chr2.4:32782363-32782382 MS.gene77097:CDS 35.0%
! CCAAGCAATTTAAAATCCTG+AGG + chr2.4:32782528-32782547 None:intergenic 35.0%
! CCTAGAAGGGTATACTTTAA+TGG - chr2.4:32784370-32784389 MS.gene77097:CDS 35.0%
! CCTCAGGATTTTAAATTGCT+TGG - chr2.4:32782525-32782544 MS.gene77097:intron 35.0%
! GATTTTAAATTGCTTGGACC+GGG - chr2.4:32782531-32782550 MS.gene77097:intron 35.0%
! GGATTTTAAATTGCTTGGAC+CGG - chr2.4:32782530-32782549 MS.gene77097:intron 35.0%
! GTGCATTGCAATAAAGGTTT+TGG + chr2.4:32783600-32783619 None:intergenic 35.0%
AACTGCGGCCACAATATAAA+AGG + chr2.4:32783422-32783441 None:intergenic 40.0%
AATTGCACGCATTCATACAG+TGG + chr2.4:32784107-32784126 None:intergenic 40.0%
AGAAATCATATGGGCCATGA+TGG - chr2.4:32782266-32782285 MS.gene77097:CDS 40.0%
ATGGACATTAGGATGGACAT+GGG - chr2.4:32782236-32782255 MS.gene77097:CDS 40.0%
ATGGCTTCATAAGGAAGAAG+TGG + chr2.4:32783801-32783820 None:intergenic 40.0%
ATTGTGTATGCAGATGACAC+AGG - chr2.4:32784220-32784239 MS.gene77097:CDS 40.0%
CAAGACAGTTAAACTACCTC+AGG - chr2.4:32782509-32782528 MS.gene77097:intron 40.0%
CTCGACAGCAGTTTAAAAGT+TGG + chr2.4:32783210-32783229 None:intergenic 40.0%
GAAATCATATGGGCCATGAT+GGG - chr2.4:32782267-32782286 MS.gene77097:CDS 40.0%
GAGCAGAATTTGGCAAGAAA+GGG + chr2.4:32784429-32784448 None:intergenic 40.0%
GATGACACAGGAATGTTCTA+TGG - chr2.4:32784232-32784251 MS.gene77097:CDS 40.0%
GGCCCATATGATTTCTTTCT+TGG + chr2.4:32782262-32782281 None:intergenic 40.0%
GGGCCAAGAAAGAAATCATA+TGG - chr2.4:32782256-32782275 MS.gene77097:CDS 40.0%
TAAAGGGAATGTGCAATCTG+AGG - chr2.4:32784294-32784313 MS.gene77097:CDS 40.0%
TAACTGCTATTGCAGCCATA+TGG + chr2.4:32783384-32783403 None:intergenic 40.0%
TACACAAACTGTTGCAAAGG+TGG - chr2.4:32782405-32782424 MS.gene77097:CDS 40.0%
TATTTCCAGCAACCTCTTTC+AGG + chr2.4:32783551-32783570 None:intergenic 40.0%
TCTTGCCAAATTCTGCTCAA+AGG - chr2.4:32784431-32784450 MS.gene77097:CDS 40.0%
TGAGCAGAATTTGGCAAGAA+AGG + chr2.4:32784430-32784449 None:intergenic 40.0%
TGCATATTGTAAGATGCTCC+AGG + chr2.4:32782384-32782403 None:intergenic 40.0%
TTCATACAGTGGCACATATG+TGG + chr2.4:32784096-32784115 None:intergenic 40.0%
! AACTGTCTTGTTAGTCCTTC+CGG + chr2.4:32782499-32782518 None:intergenic 40.0%
! AAGAAAGGGTATGAATCAGG+AGG + chr2.4:32784415-32784434 None:intergenic 40.0%
! ATCTGAGGTGCTTATGAAGA+AGG - chr2.4:32784309-32784328 MS.gene77097:CDS 40.0%
! CAACTTTTAAACTGCTGTCG+AGG - chr2.4:32783208-32783227 MS.gene77097:intron 40.0%
!! ACGCTTTAAAGAAACTGTCC+TGG - chr2.4:32782868-32782887 MS.gene77097:intron 40.0%
!! AGCGTTGATTGTAACCATCA+AGG + chr2.4:32782853-32782872 None:intergenic 40.0%
!!! TTTTTTCCTATGCAGTGTCC+TGG - chr2.4:32783009-32783028 MS.gene77097:CDS 40.0%
AACACAACAGCTTGGGTTCT+TGG + chr2.4:32783069-32783088 None:intergenic 45.0%
AAGATCCTGAAAGAGGTTGC+TGG - chr2.4:32783543-32783562 MS.gene77097:intron 45.0%
AAGGTGGTGTGCTAACATCT+TGG - chr2.4:32782421-32782440 MS.gene77097:CDS 45.0%
AAGTTGGACAAGCTGTGACT+TGG + chr2.4:32783115-32783134 None:intergenic 45.0%
AATGCACACGAACAGGACAA+AGG + chr2.4:32783629-32783648 None:intergenic 45.0%
AGCAGGATATTCATGTGGTC+CGG - chr2.4:32782554-32782573 MS.gene77097:intron 45.0%
AGGTGGTGTGCTAACATCTT+GGG - chr2.4:32782422-32782441 MS.gene77097:CDS 45.0%
ATATGCCAATGCACACGAAC+AGG + chr2.4:32783636-32783655 None:intergenic 45.0%
GATGGACATTAGGATGGACA+TGG - chr2.4:32782235-32782254 MS.gene77097:CDS 45.0%
GCAATCTCGGCATTTCAGAT+GGG - chr2.4:32782462-32782481 MS.gene77097:CDS 45.0%
GGACTTCTCTTGCAACTATG+AGG + chr2.4:32782342-32782361 None:intergenic 45.0%
GGTAGCCTTTGAGCAGAATT+TGG + chr2.4:32784439-32784458 None:intergenic 45.0%
GTTGAAGGAACTCTCTTAGC+CGG + chr2.4:32782576-32782595 None:intergenic 45.0%
TAATGGAAGCTGGGGCTAAA+GGG - chr2.4:32784278-32784297 MS.gene77097:CDS 45.0%
TGACAACCACAAGCACAAGT+TGG + chr2.4:32783131-32783150 None:intergenic 45.0%
TGCAATCTCGGCATTTCAGA+TGG - chr2.4:32782461-32782480 MS.gene77097:CDS 45.0%
TGGCGTGTGCATTGCAATAA+AGG + chr2.4:32783606-32783625 None:intergenic 45.0%
TTTGTCCTGTTCGTGTGCAT+TGG - chr2.4:32783628-32783647 MS.gene77097:CDS 45.0%
! AAAGGGTATGAATCAGGAGG+TGG + chr2.4:32784412-32784431 None:intergenic 45.0%
! ATAATGGAAGCTGGGGCTAA+AGG - chr2.4:32784277-32784296 MS.gene77097:CDS 45.0%
! GGCAAGAAAGGGTATGAATC+AGG + chr2.4:32784418-32784437 None:intergenic 45.0%
!! AAGGATGGGCTTTTCCTAGA+AGG - chr2.4:32784356-32784375 MS.gene77097:CDS 45.0%
!! AGGATGGGCTTTTCCTAGAA+GGG - chr2.4:32784357-32784376 MS.gene77097:CDS 45.0%
!! GATTAAAAAAATAAATAAAT+TGG + chr2.4:32784026-32784045 None:intergenic 5.0%
AATGCCGAGATTGCACCTGA+AGG + chr2.4:32782456-32782475 None:intergenic 50.0%
ACAACAGCTTGGGTTCTTGG+AGG + chr2.4:32783066-32783085 None:intergenic 50.0%
ATGAACCCGGGATGGACATT+AGG - chr2.4:32782225-32782244 MS.gene77097:CDS 50.0%
ATGCCGAGATTGCACCTGAA+GGG + chr2.4:32782455-32782474 None:intergenic 50.0%
CACAAAGCGACGATCACTAC+AGG - chr2.4:32782935-32782954 MS.gene77097:CDS 50.0%
CACATTCCAGGACACTGCAT+AGG + chr2.4:32783018-32783037 None:intergenic 50.0%
CCACATGAATATCCTGCTCC+CGG + chr2.4:32782552-32782571 None:intergenic 50.0%
CTGCAATACGACTGAGACTG+AGG + chr2.4:32782768-32782787 None:intergenic 50.0%
GACGGTCCAGATTTCAAGCT+TGG + chr2.4:32784056-32784075 None:intergenic 50.0%
GCTTGTCCAACTTGTGCTTG+TGG - chr2.4:32783122-32783141 MS.gene77097:CDS 50.0%
GGTGGTGTGCTAACATCTTG+GGG - chr2.4:32782423-32782442 MS.gene77097:CDS 50.0%
GTCCATCCTAATGTCCATCC+CGG + chr2.4:32782234-32782253 None:intergenic 50.0%
TCCATCCTAATGTCCATCCC+GGG + chr2.4:32782233-32782252 None:intergenic 50.0%
TCGGCATTTCAGATGGGTGT+AGG - chr2.4:32782468-32782487 MS.gene77097:CDS 50.0%
TGTGCTAACATCTTGGGGAC+AGG - chr2.4:32782428-32782447 MS.gene77097:CDS 50.0%
AAATTGCTTGGACCGGGAGC+AGG - chr2.4:32782537-32782556 MS.gene77097:intron 55.0%
ACCCGGGATGGACATTAGGA+TGG - chr2.4:32782229-32782248 MS.gene77097:CDS 55.0%
CAGATGGGTGTAGGACTCTC+CGG - chr2.4:32782477-32782496 MS.gene77097:CDS 55.0%
CCGGGAGCAGGATATTCATG+TGG - chr2.4:32782549-32782568 MS.gene77097:intron 55.0%
GCACACGAACAGGACAAAGG+TGG + chr2.4:32783626-32783645 None:intergenic 55.0%
GGACCCTTCAGGTGCAATCT+CGG - chr2.4:32782449-32782468 MS.gene77097:CDS 55.0%
GGAGCGCAAGCAACAGAACA+AGG - chr2.4:32782288-32782307 MS.gene77097:CDS 55.0%
! TGTTGCTTGCGCTCCCATCA+TGG + chr2.4:32782283-32782302 None:intergenic 55.0%
GCGTCCTGTGACTGCAACTG+CGG + chr2.4:32783437-32783456 None:intergenic 60.0%
TCTTGGGGACAGGACCCTTC+AGG - chr2.4:32782438-32782457 MS.gene77097:CDS 60.0%
TGGGTGTAGGACTCTCCGGA+AGG - chr2.4:32782481-32782500 MS.gene77097:CDS 60.0%
GTGGCCGCAGTTGCAGTCAC+AGG - chr2.4:32783430-32783449 MS.gene77097:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32782225 32784519 32782225 ID=MS.gene77097
chr2.4 mRNA 32782225 32784519 32782225 ID=MS.gene77097.t1;Parent=MS.gene77097
chr2.4 exon 32784105 32784519 32784105 ID=MS.gene77097.t1.exon1;Parent=MS.gene77097.t1
chr2.4 CDS 32784105 32784519 32784105 ID=cds.MS.gene77097.t1;Parent=MS.gene77097.t1
chr2.4 exon 32783573 32783720 32783573 ID=MS.gene77097.t1.exon2;Parent=MS.gene77097.t1
chr2.4 CDS 32783573 32783720 32783573 ID=cds.MS.gene77097.t1;Parent=MS.gene77097.t1
chr2.4 exon 32782922 32783211 32782922 ID=MS.gene77097.t1.exon3;Parent=MS.gene77097.t1
chr2.4 CDS 32782922 32783211 32782922 ID=cds.MS.gene77097.t1;Parent=MS.gene77097.t1
chr2.4 exon 32782225 32782511 32782225 ID=MS.gene77097.t1.exon4;Parent=MS.gene77097.t1
chr2.4 CDS 32782225 32782511 32782225 ID=cds.MS.gene77097.t1;Parent=MS.gene77097.t1
Gene Sequence

>MS.gene77097

ATGAACCCGGGATGGACATTAGGATGGACATGGGCCAAGAAAGAAATCATATGGGCCATGATGGGAGCGCAAGCAACAGAACAAGGAGATTGTTCAAAGTTCAAGTTAAAGATTCCTCATAGTTGCAAGAGAAGTCCTCAAGTAGTTGACTTATTACCTGGAGCATCTTACAATATGCAATACACAAACTGTTGCAAAGGTGGTGTGCTAACATCTTGGGGACAGGACCCTTCAGGTGCAATCTCGGCATTTCAGATGGGTGTAGGACTCTCCGGAAGGACTAACAAGACAGTTAAACTACCTCAGGATTTTAAATTGCTTGGACCGGGAGCAGGATATTCATGTGGTCCGGCTAAGAGAGTTCCTTCAACTGTTATTCTTACAGATGATCGTAGGCGAAAAGCTCAAGCTTTGAGTATGCATACTAATTCCCTTTGAGTATATTGCAAATAGTTATGGTTTACAATCACGATTTTAAATTGAATCTGCATCGTAATTTTTAATACTGCCGAGAATTATGGTCAAATGTAGCTGATGCAACCTCAGTCTCAGTCGTATTGCAGTCATTGTGACATCAAAACATGATGTCGCGACCTTGATTGTAATTGCAGATCCTTTTCGAAAACCTTGATGGTTACAATCAACGCTTTAAAGAAACTGTCCTGGAAATAGTATTATCTAATATTCGATAACAAAGTTTGTGATACAAACACAAAGCGACGATCACTACAGGTTAAAACCTTGAATACAAATGTACTATTAATATTATCTAATGTTCCTGTATTTTTTTCCTATGCAGTGTCCTGGAATGTGACATGTACTTATTCACAATTTCTTGCCTCCAAGAACCCAAGCTGTTGTGTTTCTTTATCAACATTCTACAATGACCAAGTCACAGCTTGTCCAACTTGTGCTTGTGGTTGTCAGAATAATGCTACTTGCGTAACGTAAGACGCCTTAATTTCTACTTCCACATTATTAGCCAACTTTTAAACTGCTGTCGAGGTTGCATCATAATCATTTATAGGACAAGAAATTCAGTCAAATACAATTGGTTGATGCAACTTCAATTGCAGTTGTAGAGACATCGTTTTGTGATGTCGCAGCGTAGATTACAAAAGCTTTCCGTGTCCAAAATCATGATTATTAGTCTTAACCATATGGCTGCAATAGCAGTTATAGAAACCTCTAAAACCTTTTATATTGTGGCCGCAGTTGCAGTCACAGGACGCAATTTAGAGCCTTATAAGTTATAACATTATTTGGAAATAGTAATGCTAATTTTGATTTGTCATTATGCAAATGCAGGAAAGATTCAAAGATCCTGAAAGAGGTTGCTGGAAATAACAAGACTAGGAAGAGTGATATCGCACCAAAACCTTTATTGCAATGCACACGCCACCTTTGTCCTGTTCGTGTGCATTGGCATATAAAAGATAATTATAAGGATTATTGGCGTGTAAAGATTGCAATCATCAACTTCAAGTATAGAATGAATTACACACAATGGGGTCTTGTTGTGCAACACCCTAATCTCAGTAATGTTACCCAAGTCTATAGTTTTGAATACATGCCACTTCTTCCTTATGAAGCCATAAGTAAGTTCTTCAAATATTAGACACCGTCTATATTTTGTTTACTTGAATTACAAAATGAAATTAACAGAACAAGAAAATGAATTTCTTATCTAACTTTGACGTCTCTAATACAAATTCGGATTGCATGCAGTCACAATTGTTGCATTGCAATCAACATGTTTGTGGTCATCCGATTAATATCGTACAATCCAAATTTCAAGCCAATTTATTTATTTTTTTAATCTAAAATACCAAGCTTGAAATCTGGACCGTCTGATCTTAATAAGACAACCACATATGTGCCACTGTATGAATGCGTGCAATTTCGAGTGCATACAATCCAAATACATATAATATCCCTATAATGCATAGAGTCACATGAAGAGTTATTGTTTTTTTAACTTTTGTGCATGGCTGAATTGTGTATGCAGATGACACAGGAATGTTCTATGGTTTGAAATTCTACAATGATTTAATAATGGAAGCTGGGGCTAAAGGGAATGTGCAATCTGAGGTGCTTATGAAGAAGGACAAGAACACATTTACACTAAAGCAAGGATGGGCTTTTCCTAGAAGGGTATACTTTAATGGTGATGAATGTATGCTTCCACCTCCTGATTCATACCCTTTCTTGCCAAATTCTGCTCAAAGGCTACCTACAAGTGTTGTAATGATGACAGCCTATGTGATTATTGCATCATTTTTCATTTGGTTTTGA
Protein sequence

>MS.gene77097.t1

MNPGWTLGWTWAKKEIIWAMMGAQATEQGDCSKFKLKIPHSCKRSPQVVDLLPGASYNMQYTNCCKGGVLTSWGQDPSGAISAFQMGVGLSGRTNKTVKLPQDFKLLGPGAGYSCGPAKRVPSTVILTDDRRRKAQALMSWNVTCTYSQFLASKNPSCCVSLSTFYNDQVTACPTCACGCQNNATCVTKDSKILKEVAGNNKTRKSDIAPKPLLQCTRHLCPVRVHWHIKDNYKDYWRVKIAIINFKYRMNYTQWGLVVQHPNLSNVTQVYSFEYMPLLPYEAINDTGMFYGLKFYNDLIMEAGAKGNVQSEVLMKKDKNTFTLKQGWAFPRRVYFNGDECMLPPPDSYPFLPNSAQRLPTSVVMMTAYVIIASFFIWF