AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77098

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 109 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCCCATATGATTTCTTTCT+TGG 0.151399 2.4:+32775523 None:intergenic
CCTAGAAGGGTATACTTTAA+TGG 0.184050 2.4:-32773412 MS.gene77098:CDS
TATTTCCAGCAACCTCTTTC+AGG 0.198489 2.4:+32774234 None:intergenic
AAGATAATTATAAGGATTAT+TGG 0.226624 2.4:-32774124 MS.gene77098:CDS
AACACAACAGCTTGGGTTCT+TGG 0.235897 2.4:+32774716 None:intergenic
GGTAGCCTTTGAGCAGAATT+TGG 0.247873 2.4:+32773346 None:intergenic
CCATTAAAGTATACCCTTCT+AGG 0.259971 2.4:+32773412 None:intergenic
AACTGTCTTGTTAGTCCTTC+CGG 0.267626 2.4:+32775286 None:intergenic
TGGCGTGTGCATTGCAATAA+AGG 0.319433 2.4:+32774179 None:intergenic
ATTCTACAATGATTTAATAA+TGG 0.323384 2.4:-32773521 MS.gene77098:CDS
GCATATAAAAGATAATTATA+AGG 0.335357 2.4:-32774132 MS.gene77098:CDS
GTGCATTGCAATAAAGGTTT+TGG 0.357973 2.4:+32774185 None:intergenic
GGGTAACATTACTGAGATTA+GGG 0.358595 2.4:+32774030 None:intergenic
TAATGGAAGCTGGGGCTAAA+GGG 0.368160 2.4:-32773504 MS.gene77098:CDS
GATGACACAGGAATGTTCTA+TGG 0.385600 2.4:-32773550 MS.gene77098:CDS
TGTTGCTTGCGCTCCCATCA+TGG 0.386890 2.4:+32775502 None:intergenic
ATGGACATCAGATGGTTATA+TGG 0.392606 2.4:-32776191 MS.gene77098:intron
ATGAACCCGGGATGGACATT+AGG 0.394711 2.4:-32775557 MS.gene77098:CDS
ATCCCGGGTTCATAATGTTT+CGG 0.395348 2.4:+32775567 None:intergenic
AGGTGGTGTGCTAACATCTT+GGG 0.396771 2.4:-32775360 MS.gene77098:CDS
TGGGTAACATTACTGAGATT+AGG 0.404033 2.4:+32774029 None:intergenic
TTTGATCCATTGGATCCAAC+TGG 0.408485 2.4:-32776244 MS.gene77098:CDS
CAAGTAGTTGACTTATTACC+TGG 0.416180 2.4:-32775419 MS.gene77098:CDS
GGGCCAAGAAAGAAATCATA+TGG 0.421488 2.4:-32775526 MS.gene77098:CDS
TGAGCAGAATTTGGCAAGAA+AGG 0.426117 2.4:+32773355 None:intergenic
TAGAATGAATTACACACAAT+GGG 0.426364 2.4:-32774069 MS.gene77098:CDS
ATATGCCAATGCACACGAAC+AGG 0.426620 2.4:+32774149 None:intergenic
ATAATGGAAGCTGGGGCTAA+AGG 0.427586 2.4:-32773505 MS.gene77098:CDS
TCTTGGGGACAGGACCCTTC+AGG 0.429022 2.4:-32775344 MS.gene77098:CDS
AACTTACTTATGGCTTCATA+AGG 0.429069 2.4:+32773975 None:intergenic
GGCAAGAAAGGGTATGAATC+AGG 0.435645 2.4:+32773367 None:intergenic
TGTAGAGGCATTTGATCCAT+TGG 0.442199 2.4:-32776254 None:intergenic
AATGATTTAATAATGGAAGC+TGG 0.446216 2.4:-32773514 MS.gene77098:CDS
TCTGTAAGAATAACAGTTGA+AGG 0.452263 2.4:+32775194 None:intergenic
AAGATCCTGAAAGAGGTTGC+TGG 0.460769 2.4:-32774239 MS.gene77098:CDS
AAATTGCTTGGACCGGGAGC+AGG 0.463331 2.4:-32775245 MS.gene77098:CDS
GAGCAGAATTTGGCAAGAAA+GGG 0.471958 2.4:+32773356 None:intergenic
AGAAATCATATGGGCCATGA+TGG 0.480849 2.4:-32775516 MS.gene77098:CDS
ATGATTTAATAATGGAAGCT+GGG 0.480905 2.4:-32773513 MS.gene77098:CDS
ATAAAGAAACACAACAGCTT+GGG 0.481011 2.4:+32774709 None:intergenic
GTCCATCCTAATGTCCATCC+CGG 0.482281 2.4:+32775551 None:intergenic
TTTGTCCTGTTCGTGTGCAT+TGG 0.488987 2.4:-32774154 MS.gene77098:CDS
TGCAATCTCGGCATTTCAGA+TGG 0.490026 2.4:-32775321 MS.gene77098:CDS
GGACTTCTCTTGCAACTATG+AGG 0.491344 2.4:+32775443 None:intergenic
ATAAGTACATGTCACATTCC+AGG 0.497470 2.4:+32774755 None:intergenic
GATAAAGAAACACAACAGCT+TGG 0.498328 2.4:+32774708 None:intergenic
AGCAGGATATTCATGTGGTC+CGG 0.500729 2.4:-32775228 MS.gene77098:CDS
ACATTCCCAGTTGGATCCAA+TGG 0.502942 2.4:+32776238 None:intergenic
CTGGGAATGTGACAATAAGA+TGG 0.503167 2.4:-32776225 MS.gene77098:CDS
GGACCCTTCAGGTGCAATCT+CGG 0.506260 2.4:-32775333 MS.gene77098:CDS
CAAGACAGTTAAACTACCTC+AGG 0.509450 2.4:-32775273 MS.gene77098:CDS
CAGATGGGTGTAGGACTCTC+CGG 0.515620 2.4:-32775305 MS.gene77098:CDS
ATCTGAGGTGCTTATGAAGA+AGG 0.516290 2.4:-32773473 MS.gene77098:CDS
AAGGTGGTGTGCTAACATCT+TGG 0.517435 2.4:-32775361 MS.gene77098:CDS
GTCATCATTACAACACTTGT+AGG 0.519462 2.4:+32773325 None:intergenic
CATTTACACTAAAGCAAGGA+TGG 0.523081 2.4:-32773441 MS.gene77098:CDS
ACAACAGCTTGGGTTCTTGG+AGG 0.528958 2.4:+32774719 None:intergenic
CTTATTGTCACATTCCCAGT+TGG 0.532085 2.4:+32776229 None:intergenic
TGTATTCAAAACTATAGACT+TGG 0.537143 2.4:+32774009 None:intergenic
GCTTGTCCAACTTGTGCTTG+TGG 0.548007 2.4:-32774660 MS.gene77098:CDS
GGTAATAAGTCAACTACTTG+AGG 0.550335 2.4:+32775422 None:intergenic
TCTTGCCAAATTCTGCTCAA+AGG 0.554568 2.4:-32773351 MS.gene77098:CDS
AAATGATGCAATAATCACAT+AGG 0.564180 2.4:+32773300 None:intergenic
TGCATATTGTAAGATGCTCC+AGG 0.567353 2.4:+32775401 None:intergenic
GAAATCATATGGGCCATGAT+GGG 0.570678 2.4:-32775515 MS.gene77098:CDS
TGTGCTAACATCTTGGGGAC+AGG 0.571959 2.4:-32775354 MS.gene77098:CDS
TACACAAACTGTTGCAAAGG+TGG 0.573020 2.4:-32775377 MS.gene77098:CDS
ATGCCGAGATTGCACCTGAA+GGG 0.577152 2.4:+32775330 None:intergenic
TTGCTGGAAATAACAAGACT+AGG 0.578763 2.4:-32774223 MS.gene77098:CDS
CCACATGAATATCCTGCTCC+CGG 0.579683 2.4:+32775233 None:intergenic
GTTGAAGGAACTCTCTTAGC+CGG 0.580085 2.4:+32775209 None:intergenic
GGAGCGCAAGCAACAGAACA+AGG 0.580360 2.4:-32775494 MS.gene77098:CDS
CAATACACAAACTGTTGCAA+AGG 0.581528 2.4:-32775380 MS.gene77098:CDS
TCGGCATTTCAGATGGGTGT+AGG 0.583929 2.4:-32775314 MS.gene77098:CDS
TGATTTAATAATGGAAGCTG+GGG 0.584574 2.4:-32773512 MS.gene77098:CDS
TGACAACCACAAGCACAAGT+TGG 0.585659 2.4:+32774654 None:intergenic
TGGGAATGTGACAATAAGAT+GGG 0.590389 2.4:-32776224 MS.gene77098:CDS
ATGGCTTCATAAGGAAGAAG+TGG 0.591100 2.4:+32773984 None:intergenic
TCCATCCTAATGTCCATCCC+GGG 0.595187 2.4:+32775552 None:intergenic
AGATTCAAAGATCCTGAAAG+AGG 0.596172 2.4:-32774246 MS.gene77098:CDS
AAGTTGGACAAGCTGTGACT+TGG 0.597255 2.4:+32774670 None:intergenic
AATGCCGAGATTGCACCTGA+AGG 0.597959 2.4:+32775329 None:intergenic
AAAGGGTATGAATCAGGAGG+TGG 0.602047 2.4:+32773373 None:intergenic
GGCCAAGAAAGAAATCATAT+GGG 0.602816 2.4:-32775525 MS.gene77098:CDS
ATTATGTCATGGACATCAGA+TGG 0.603721 2.4:-32776199 MS.gene77098:CDS
GATGGACATTAGGATGGACA+TGG 0.605342 2.4:-32775547 MS.gene77098:CDS
GTATTCAAAACTATAGACTT+GGG 0.605359 2.4:+32774010 None:intergenic
AACACATTTACACTAAAGCA+AGG 0.605862 2.4:-32773445 MS.gene77098:CDS
ATACCGAAACATTATGAACC+CGG 0.606089 2.4:-32775570 MS.gene77098:CDS
GCAATCTCGGCATTTCAGAT+GGG 0.606491 2.4:-32775320 MS.gene77098:CDS
ACCCGGGATGGACATTAGGA+TGG 0.607366 2.4:-32775553 MS.gene77098:CDS
TACCGAAACATTATGAACCC+GGG 0.608883 2.4:-32775569 MS.gene77098:CDS
ATTTACACTAAAGCAAGGAT+GGG 0.609581 2.4:-32773440 MS.gene77098:CDS
TTATTCTTACAGATGATCGT+AGG 0.609804 2.4:-32775184 MS.gene77098:CDS
TTGATCCATTGGATCCAACT+GGG 0.610985 2.4:-32776243 MS.gene77098:CDS
GAAACATTATGAACCCGGGA+TGG 0.613102 2.4:-32775565 MS.gene77098:CDS
TAAGATGGGATATTATGTCA+TGG 0.615769 2.4:-32776210 MS.gene77098:CDS
GGTGGTGTGCTAACATCTTG+GGG 0.624710 2.4:-32775359 MS.gene77098:CDS
ATAGAATGAATTACACACAA+TGG 0.628977 2.4:-32774070 MS.gene77098:CDS
TAAAGGGAATGTGCAATCTG+AGG 0.631122 2.4:-32773488 MS.gene77098:CDS
CCAAGCAATTTAAAATCCTG+AGG 0.636131 2.4:+32775257 None:intergenic
TGGGTGTAGGACTCTCCGGA+AGG 0.662739 2.4:-32775301 MS.gene77098:CDS
AATGCACACGAACAGGACAA+AGG 0.669891 2.4:+32774156 None:intergenic
CCGGGAGCAGGATATTCATG+TGG 0.685860 2.4:-32775233 MS.gene77098:CDS
GCACACGAACAGGACAAAGG+TGG 0.685930 2.4:+32774159 None:intergenic
AAGAAAGGGTATGAATCAGG+AGG 0.687329 2.4:+32773370 None:intergenic
ATGGACATTAGGATGGACAT+GGG 0.698791 2.4:-32775546 MS.gene77098:CDS
AGAATGAATTACACACAATG+GGG 0.725776 2.4:-32774068 MS.gene77098:CDS
ATTGTGTATGCAGATGACAC+AGG 0.753079 2.4:-32773562 MS.gene77098:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAATGTTATAACTTATA+AGG + chr2.4:32775221-32775240 None:intergenic 10.0%
!! TTATAAGTTATAACATTATT+TGG - chr2.4:32775220-32775239 MS.gene77098:CDS 10.0%
!!! TTTTGATAATTTTATTCTTT+TGG + chr2.4:32773499-32773518 None:intergenic 10.0%
!! AAGATAATTATAAGGATTAT+TGG - chr2.4:32775410-32775429 MS.gene77098:CDS 15.0%
!! ATTCTACAATGATTTAATAA+TGG - chr2.4:32776013-32776032 MS.gene77098:intron 15.0%
!! GCATATAAAAGATAATTATA+AGG - chr2.4:32775402-32775421 MS.gene77098:CDS 15.0%
!! ATAGGGATATTATATGTATT+TGG + chr2.4:32775897-32775916 None:intergenic 20.0%
!! ATATTTGAAGAACTTACTTA+TGG + chr2.4:32775572-32775591 None:intergenic 20.0%
!! TAATAGTACATTTGTATTCA+AGG + chr2.4:32774719-32774738 None:intergenic 20.0%
!! TATTAGATAATACTATTTCC+AGG + chr2.4:32774641-32774660 None:intergenic 20.0%
!! TCAAGTAAACAAAATATAGA+CGG + chr2.4:32775601-32775620 None:intergenic 20.0%
!! TTAATATTCGCATAATAACA+CGG - chr2.4:32773676-32773695 MS.gene77098:intron 20.0%
!!! AAATAAATTGGCTTGAAATT+TGG + chr2.4:32775766-32775785 None:intergenic 20.0%
!!! AAGACTAATAATCATGATTT+TGG + chr2.4:32775111-32775130 None:intergenic 20.0%
!!! CGAAAAACATATTTTCAAAA+TGG - chr2.4:32773762-32773781 MS.gene77098:intron 20.0%
!!! TTATTATGTGTTACTATATC+AGG + chr2.4:32773629-32773648 None:intergenic 20.0%
!!! TTATTGCATCATTTTTCATT+TGG - chr2.4:32776243-32776262 MS.gene77098:CDS 20.0%
! AAATGATGCAATAATCACAT+AGG + chr2.4:32776237-32776256 None:intergenic 25.0%
! ACTATTTGCAATATACTCAA+AGG + chr2.4:32774411-32774430 None:intergenic 25.0%
! AGTAACTGAGATTCATTTAA+AGG + chr2.4:32773793-32773812 None:intergenic 25.0%
! ATAGAATGAATTACACACAA+TGG - chr2.4:32775464-32775483 MS.gene77098:CDS 25.0%
! CTAGAAAAGGATTTGAATAA+TGG - chr2.4:32773587-32773606 MS.gene77098:intron 25.0%
! CTATTTGCAATATACTCAAA+GGG + chr2.4:32774410-32774429 None:intergenic 25.0%
! GAAATTCAGTCAAATACAAT+TGG - chr2.4:32775009-32775028 MS.gene77098:intron 25.0%
! GAGTATATTGCAAATAGTTA+TGG - chr2.4:32774413-32774432 MS.gene77098:intron 25.0%
! GTATTCAAAACTATAGACTT+GGG + chr2.4:32775527-32775546 None:intergenic 25.0%
! GTTGCATCATAATCATTTAT+AGG - chr2.4:32774982-32775001 MS.gene77098:intron 25.0%
! TAATGTGGAAGTAGAAATTA+AGG + chr2.4:32774935-32774954 None:intergenic 25.0%
! TAGAATGAATTACACACAAT+GGG - chr2.4:32775465-32775484 MS.gene77098:CDS 25.0%
! TGTATTCAAAACTATAGACT+TGG + chr2.4:32775528-32775547 None:intergenic 25.0%
! TTAAAAGTTGGCTAATAATG+TGG + chr2.4:32774950-32774969 None:intergenic 25.0%
! TTGGATTGTACGATATTAAT+CGG + chr2.4:32775747-32775766 None:intergenic 25.0%
!! AATAATCATGATTTTGGACA+CGG + chr2.4:32775105-32775124 None:intergenic 25.0%
!! AATGATTTAATAATGGAAGC+TGG - chr2.4:32776020-32776039 MS.gene77098:intron 25.0%
!! ATGATTTAATAATGGAAGCT+GGG - chr2.4:32776021-32776040 MS.gene77098:intron 25.0%
!! GATACTATGATGTCAAATTT+TGG - chr2.4:32773909-32773928 MS.gene77098:intron 25.0%
!!! CACAATATAAAAGGTTTTAG+AGG + chr2.4:32775165-32775184 None:intergenic 25.0%
!!! CATCATTTTTGGAAACATTT+TGG + chr2.4:32773453-32773472 None:intergenic 25.0%
!!! CATTTTTCATTTGGTTTTGA+AGG - chr2.4:32776252-32776271 MS.gene77098:CDS 25.0%
!!! CTCTAAAACCTTTTATATTG+TGG - chr2.4:32775163-32775182 MS.gene77098:intron 25.0%
!!! GATTATTAGTCTTAACCATA+TGG - chr2.4:32775118-32775137 MS.gene77098:intron 25.0%
!!! GTTTTTTTAACTTTTGTGCA+TGG - chr2.4:32775945-32775964 MS.gene77098:intron 25.0%
AAAATACCAAGCTTGAAATC+TGG - chr2.4:32775799-32775818 MS.gene77098:intron 30.0%
AACACATTTACACTAAAGCA+AGG - chr2.4:32776089-32776108 MS.gene77098:intron 30.0%
AACTTACTTATGGCTTCATA+AGG + chr2.4:32775562-32775581 None:intergenic 30.0%
ACTGCATAGGAAAAAAATAC+AGG + chr2.4:32774757-32774776 None:intergenic 30.0%
AGAATGAATTACACACAATG+GGG - chr2.4:32775466-32775485 MS.gene77098:CDS 30.0%
ATAAAGAAACACAACAGCTT+GGG + chr2.4:32774828-32774847 None:intergenic 30.0%
ATGTGACTCTATGCATTATA+GGG + chr2.4:32775914-32775933 None:intergenic 30.0%
ATTTACACTAAAGCAAGGAT+GGG - chr2.4:32776094-32776113 MS.gene77098:intron 30.0%
ATTTGTCATTATGCAAATGC+AGG - chr2.4:32775263-32775282 MS.gene77098:CDS 30.0%
CTACATTTGACCATAATTCT+CGG + chr2.4:32774488-32774507 None:intergenic 30.0%
GAAAAGGATTTGAATAATGG+TGG - chr2.4:32773590-32773609 MS.gene77098:intron 30.0%
TAAGATGGGATATTATGTCA+TGG - chr2.4:32773324-32773343 MS.gene77098:CDS 30.0%
TCCAAAAATGATGAAATCAC+TGG - chr2.4:32773460-32773479 MS.gene77098:CDS 30.0%
TCTGTAAGAATAACAGTTGA+AGG + chr2.4:32774343-32774362 None:intergenic 30.0%
TGACGTCTCTAATACAAATT+CGG - chr2.4:32775671-32775690 MS.gene77098:intron 30.0%
TTATTCTTACAGATGATCGT+AGG - chr2.4:32774350-32774369 MS.gene77098:intron 30.0%
TTTAATACTGCCGAGAATTA+TGG - chr2.4:32774475-32774494 MS.gene77098:intron 30.0%
! ACCAGTGATTTCATCATTTT+TGG + chr2.4:32773464-32773483 None:intergenic 30.0%
! CTGATCATGCTATTCTTTTA+AGG - chr2.4:32773387-32773406 MS.gene77098:CDS 30.0%
! GTTGTCTTATTAAGATCAGA+CGG + chr2.4:32775826-32775845 None:intergenic 30.0%
! TGATTTAATAATGGAAGCTG+GGG - chr2.4:32776022-32776041 MS.gene77098:intron 30.0%
AATGAATCTCAGTTACTTCG+AGG - chr2.4:32773796-32773815 MS.gene77098:intron 35.0%
AGATTCAAAGATCCTGAAAG+AGG - chr2.4:32775288-32775307 MS.gene77098:CDS 35.0%
AGGATCTGCAATTACAATCA+AGG + chr2.4:32774573-32774592 None:intergenic 35.0%
ATAAGTACATGTCACATTCC+AGG + chr2.4:32774782-32774801 None:intergenic 35.0%
ATACCGAAACATTATGAACC+CGG - chr2.4:32773964-32773983 MS.gene77098:intron 35.0%
ATGAATCTCAGTTACTTCGA+GGG - chr2.4:32773797-32773816 MS.gene77098:intron 35.0%
ATGGACATCAGATGGTTATA+TGG - chr2.4:32773343-32773362 MS.gene77098:CDS 35.0%
ATTATGTCATGGACATCAGA+TGG - chr2.4:32773335-32773354 MS.gene77098:CDS 35.0%
CAATACACAAACTGTTGCAA+AGG - chr2.4:32774154-32774173 MS.gene77098:CDS 35.0%
CATGTGACTCTATGCATTAT+AGG + chr2.4:32775915-32775934 None:intergenic 35.0%
CATTGCAATCAACATGTTTG+TGG - chr2.4:32775717-32775736 MS.gene77098:intron 35.0%
CATTTACACTAAAGCAAGGA+TGG - chr2.4:32776093-32776112 MS.gene77098:intron 35.0%
CCATTAAAGTATACCCTTCT+AGG + chr2.4:32776125-32776144 None:intergenic 35.0%
GATAAAGAAACACAACAGCT+TGG + chr2.4:32774829-32774848 None:intergenic 35.0%
GGCCAAGAAAGAAATCATAT+GGG - chr2.4:32774009-32774028 MS.gene77098:CDS 35.0%
GGGTAACATTACTGAGATTA+GGG + chr2.4:32775507-32775526 None:intergenic 35.0%
GGTAATAAGTCAACTACTTG+AGG + chr2.4:32774115-32774134 None:intergenic 35.0%
GTCATCATTACAACACTTGT+AGG + chr2.4:32776212-32776231 None:intergenic 35.0%
GTGTAACACACATAGATGTT+TGG - chr2.4:32773698-32773717 MS.gene77098:intron 35.0%
TAGATTCGAGAAGCTAGAAA+AGG - chr2.4:32773574-32773593 MS.gene77098:intron 35.0%
TAGCATGATCAGACAAAGTT+TGG + chr2.4:32773379-32773398 None:intergenic 35.0%
TGGGAATGTGACAATAAGAT+GGG - chr2.4:32773310-32773329 MS.gene77098:CDS 35.0%
TGGGTAACATTACTGAGATT+AGG + chr2.4:32775508-32775527 None:intergenic 35.0%
TTAATCTCCACAATTACACG+TGG - chr2.4:32773821-32773840 MS.gene77098:intron 35.0%
TTGCTGGAAATAACAAGACT+AGG - chr2.4:32775311-32775330 MS.gene77098:CDS 35.0%
! AACCATCAAGGTTTTCGAAA+AGG + chr2.4:32774593-32774612 None:intergenic 35.0%
! ATCCTTTTCGAAAACCTTGA+TGG - chr2.4:32774588-32774607 MS.gene77098:intron 35.0%
! CAAGTAGTTGACTTATTACC+TGG - chr2.4:32774115-32774134 MS.gene77098:CDS 35.0%
! CCAAGCAATTTAAAATCCTG+AGG + chr2.4:32774280-32774299 None:intergenic 35.0%
! CCTAGAAGGGTATACTTTAA+TGG - chr2.4:32776122-32776141 MS.gene77098:intron 35.0%
! CCTCAGGATTTTAAATTGCT+TGG - chr2.4:32774277-32774296 MS.gene77098:intron 35.0%
! GATTTTAAATTGCTTGGACC+GGG - chr2.4:32774283-32774302 MS.gene77098:intron 35.0%
! GGATTTTAAATTGCTTGGAC+CGG - chr2.4:32774282-32774301 MS.gene77098:intron 35.0%
! GTGCATTGCAATAAAGGTTT+TGG + chr2.4:32775352-32775371 None:intergenic 35.0%
!! ATATGTTTTTCGATACGCAC+TGG + chr2.4:32773754-32773773 None:intergenic 35.0%
!! CTATGATGTCAAATTTTGGC+AGG - chr2.4:32773913-32773932 MS.gene77098:intron 35.0%
AACTGCGGCCACAATATAAA+AGG + chr2.4:32775174-32775193 None:intergenic 40.0%
AATTGCACGCATTCATACAG+TGG + chr2.4:32775859-32775878 None:intergenic 40.0%
ACACGAACTCCTATGGTTAA+GGG + chr2.4:32773726-32773745 None:intergenic 40.0%
AGAAATCATATGGGCCATGA+TGG - chr2.4:32774018-32774037 MS.gene77098:CDS 40.0%
ATCCCGGGTTCATAATGTTT+CGG + chr2.4:32773970-32773989 None:intergenic 40.0%
ATGGACATTAGGATGGACAT+GGG - chr2.4:32773988-32774007 MS.gene77098:CDS 40.0%
ATGGCTTCATAAGGAAGAAG+TGG + chr2.4:32775553-32775572 None:intergenic 40.0%
ATTGTGTATGCAGATGACAC+AGG - chr2.4:32775972-32775991 MS.gene77098:intron 40.0%
CAAGACAGTTAAACTACCTC+AGG - chr2.4:32774261-32774280 MS.gene77098:intron 40.0%
CTATCATCCACGTGTAATTG+TGG + chr2.4:32773831-32773850 None:intergenic 40.0%
CTCGACAGCAGTTTAAAAGT+TGG + chr2.4:32774962-32774981 None:intergenic 40.0%
CTGGGAATGTGACAATAAGA+TGG - chr2.4:32773309-32773328 MS.gene77098:CDS 40.0%
CTTATTGTCACATTCCCAGT+TGG + chr2.4:32773308-32773327 None:intergenic 40.0%
GAAATCATATGGGCCATGAT+GGG - chr2.4:32774019-32774038 MS.gene77098:CDS 40.0%
GAGCAGAATTTGGCAAGAAA+GGG + chr2.4:32776181-32776200 None:intergenic 40.0%
GATGACACAGGAATGTTCTA+TGG - chr2.4:32775984-32776003 MS.gene77098:intron 40.0%
GGCCCATATGATTTCTTTCT+TGG + chr2.4:32774014-32774033 None:intergenic 40.0%
GGGCCAAGAAAGAAATCATA+TGG - chr2.4:32774008-32774027 MS.gene77098:CDS 40.0%
TAAAGGGAATGTGCAATCTG+AGG - chr2.4:32776046-32776065 MS.gene77098:intron 40.0%
TAACTGCTATTGCAGCCATA+TGG + chr2.4:32775136-32775155 None:intergenic 40.0%
TACACAAACTGTTGCAAAGG+TGG - chr2.4:32774157-32774176 MS.gene77098:CDS 40.0%
TACCGAAACATTATGAACCC+GGG - chr2.4:32773965-32773984 MS.gene77098:intron 40.0%
TATTTCCAGCAACCTCTTTC+AGG + chr2.4:32775303-32775322 None:intergenic 40.0%
TCTTGCCAAATTCTGCTCAA+AGG - chr2.4:32776183-32776202 MS.gene77098:intron 40.0%
TGAATCTCAGTTACTTCGAG+GGG - chr2.4:32773798-32773817 MS.gene77098:intron 40.0%
TGAGCAGAATTTGGCAAGAA+AGG + chr2.4:32776182-32776201 None:intergenic 40.0%
TGCATATTGTAAGATGCTCC+AGG + chr2.4:32774136-32774155 None:intergenic 40.0%
TGTTTGGATCCCTTAACCAT+AGG - chr2.4:32773714-32773733 MS.gene77098:intron 40.0%
TTCATACAGTGGCACATATG+TGG + chr2.4:32775848-32775867 None:intergenic 40.0%
TTGATCCATTGGATCCAACT+GGG - chr2.4:32773291-32773310 MS.gene77098:CDS 40.0%
TTTGATCCATTGGATCCAAC+TGG - chr2.4:32773290-32773309 MS.gene77098:CDS 40.0%
! AACTGTCTTGTTAGTCCTTC+CGG + chr2.4:32774251-32774270 None:intergenic 40.0%
! AAGAAAGGGTATGAATCAGG+AGG + chr2.4:32776167-32776186 None:intergenic 40.0%
! ATCTGAGGTGCTTATGAAGA+AGG - chr2.4:32776061-32776080 MS.gene77098:intron 40.0%
! CAACTTTTAAACTGCTGTCG+AGG - chr2.4:32774960-32774979 MS.gene77098:intron 40.0%
!! ACGCTTTAAAGAAACTGTCC+TGG - chr2.4:32774620-32774639 MS.gene77098:intron 40.0%
!! AGCGTTGATTGTAACCATCA+AGG + chr2.4:32774605-32774624 None:intergenic 40.0%
!!! TTTTTTCCTATGCAGTGTCC+TGG - chr2.4:32774761-32774780 MS.gene77098:CDS 40.0%
AACACAACAGCTTGGGTTCT+TGG + chr2.4:32774821-32774840 None:intergenic 45.0%
AACCATAGGAGTTCGTGTCA+TGG - chr2.4:32773728-32773747 MS.gene77098:intron 45.0%
AAGATCCTGAAAGAGGTTGC+TGG - chr2.4:32775295-32775314 MS.gene77098:CDS 45.0%
AAGGTGGTGTGCTAACATCT+TGG - chr2.4:32774173-32774192 MS.gene77098:CDS 45.0%
AAGTTGGACAAGCTGTGACT+TGG + chr2.4:32774867-32774886 None:intergenic 45.0%
AATGCACACGAACAGGACAA+AGG + chr2.4:32775381-32775400 None:intergenic 45.0%
ACATTCCCAGTTGGATCCAA+TGG + chr2.4:32773299-32773318 None:intergenic 45.0%
AGCAGGATATTCATGTGGTC+CGG - chr2.4:32774306-32774325 MS.gene77098:intron 45.0%
AGGTGGTGTGCTAACATCTT+GGG - chr2.4:32774174-32774193 MS.gene77098:CDS 45.0%
ATATGCCAATGCACACGAAC+AGG + chr2.4:32775388-32775407 None:intergenic 45.0%
GAAACATTATGAACCCGGGA+TGG - chr2.4:32773969-32773988 MS.gene77098:intron 45.0%
GACACGAACTCCTATGGTTA+AGG + chr2.4:32773727-32773746 None:intergenic 45.0%
GATGGACATTAGGATGGACA+TGG - chr2.4:32773987-32774006 MS.gene77098:CDS 45.0%
GCAATCTCGGCATTTCAGAT+GGG - chr2.4:32774214-32774233 MS.gene77098:CDS 45.0%
GGACTTCTCTTGCAACTATG+AGG + chr2.4:32774094-32774113 None:intergenic 45.0%
GGTAGCCTTTGAGCAGAATT+TGG + chr2.4:32776191-32776210 None:intergenic 45.0%
GTTGAAGGAACTCTCTTAGC+CGG + chr2.4:32774328-32774347 None:intergenic 45.0%
TAATGGAAGCTGGGGCTAAA+GGG - chr2.4:32776030-32776049 MS.gene77098:intron 45.0%
TGACAACCACAAGCACAAGT+TGG + chr2.4:32774883-32774902 None:intergenic 45.0%
TGCAATCTCGGCATTTCAGA+TGG - chr2.4:32774213-32774232 MS.gene77098:CDS 45.0%
TGGCGTGTGCATTGCAATAA+AGG + chr2.4:32775358-32775377 None:intergenic 45.0%
TTTGTCCTGTTCGTGTGCAT+TGG - chr2.4:32775380-32775399 MS.gene77098:CDS 45.0%
! AAAGGGTATGAATCAGGAGG+TGG + chr2.4:32776164-32776183 None:intergenic 45.0%
! ATAATGGAAGCTGGGGCTAA+AGG - chr2.4:32776029-32776048 MS.gene77098:intron 45.0%
! GGCAAGAAAGGGTATGAATC+AGG + chr2.4:32776170-32776189 None:intergenic 45.0%
!! AAGGATGGGCTTTTCCTAGA+AGG - chr2.4:32776108-32776127 MS.gene77098:intron 45.0%
!! AGGATGGGCTTTTCCTAGAA+GGG - chr2.4:32776109-32776128 MS.gene77098:intron 45.0%
!! GATTAAAAAAATAAATAAAT+TGG + chr2.4:32775778-32775797 None:intergenic 5.0%
AATGCCGAGATTGCACCTGA+AGG + chr2.4:32774208-32774227 None:intergenic 50.0%
ACAACAGCTTGGGTTCTTGG+AGG + chr2.4:32774818-32774837 None:intergenic 50.0%
ATGAACCCGGGATGGACATT+AGG - chr2.4:32773977-32773996 MS.gene77098:intron 50.0%
ATGCCGAGATTGCACCTGAA+GGG + chr2.4:32774207-32774226 None:intergenic 50.0%
CACAAAGCGACGATCACTAC+AGG - chr2.4:32774687-32774706 MS.gene77098:CDS 50.0%
CACATTCCAGGACACTGCAT+AGG + chr2.4:32774770-32774789 None:intergenic 50.0%
CCACATGAATATCCTGCTCC+CGG + chr2.4:32774304-32774323 None:intergenic 50.0%
CTGCAATACGACTGAGACTG+AGG + chr2.4:32774520-32774539 None:intergenic 50.0%
GACGGTCCAGATTTCAAGCT+TGG + chr2.4:32775808-32775827 None:intergenic 50.0%
GCTTGTCCAACTTGTGCTTG+TGG - chr2.4:32774874-32774893 MS.gene77098:intron 50.0%
GGTGGTGTGCTAACATCTTG+GGG - chr2.4:32774175-32774194 MS.gene77098:CDS 50.0%
GTCCATCCTAATGTCCATCC+CGG + chr2.4:32773986-32774005 None:intergenic 50.0%
TCCATCCTAATGTCCATCCC+GGG + chr2.4:32773985-32774004 None:intergenic 50.0%
TCGGCATTTCAGATGGGTGT+AGG - chr2.4:32774220-32774239 MS.gene77098:CDS 50.0%
TGTGCTAACATCTTGGGGAC+AGG - chr2.4:32774180-32774199 MS.gene77098:CDS 50.0%
AAATTGCTTGGACCGGGAGC+AGG - chr2.4:32774289-32774308 MS.gene77098:intron 55.0%
ACCCGGGATGGACATTAGGA+TGG - chr2.4:32773981-32774000 MS.gene77098:intron 55.0%
CAGATGGGTGTAGGACTCTC+CGG - chr2.4:32774229-32774248 MS.gene77098:CDS 55.0%
CCGGGAGCAGGATATTCATG+TGG - chr2.4:32774301-32774320 MS.gene77098:intron 55.0%
GCACACGAACAGGACAAAGG+TGG + chr2.4:32775378-32775397 None:intergenic 55.0%
GGACCCTTCAGGTGCAATCT+CGG - chr2.4:32774201-32774220 MS.gene77098:CDS 55.0%
GGAGCGCAAGCAACAGAACA+AGG - chr2.4:32774040-32774059 MS.gene77098:CDS 55.0%
GGCCATGACACGAACTCCTA+TGG + chr2.4:32773733-32773752 None:intergenic 55.0%
! TGTTGCTTGCGCTCCCATCA+TGG + chr2.4:32774035-32774054 None:intergenic 55.0%
GCGTCCTGTGACTGCAACTG+CGG + chr2.4:32775189-32775208 None:intergenic 60.0%
TCTTGGGGACAGGACCCTTC+AGG - chr2.4:32774190-32774209 MS.gene77098:CDS 60.0%
TGGGTGTAGGACTCTCCGGA+AGG - chr2.4:32774233-32774252 MS.gene77098:CDS 60.0%
GTGGCCGCAGTTGCAGTCAC+AGG - chr2.4:32775182-32775201 MS.gene77098:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32773285 32776271 32773285 ID=MS.gene77098
chr2.4 mRNA 32773285 32776271 32773285 ID=MS.gene77098.t1;Parent=MS.gene77098
chr2.4 exon 32776192 32776271 32776192 ID=MS.gene77098.t1.exon1;Parent=MS.gene77098.t1
chr2.4 CDS 32776192 32776271 32776192 ID=cds.MS.gene77098.t1;Parent=MS.gene77098.t1
chr2.4 exon 32775165 32775621 32775165 ID=MS.gene77098.t1.exon2;Parent=MS.gene77098.t1
chr2.4 CDS 32775165 32775621 32775165 ID=cds.MS.gene77098.t1;Parent=MS.gene77098.t1
chr2.4 exon 32774633 32774780 32774633 ID=MS.gene77098.t1.exon3;Parent=MS.gene77098.t1
chr2.4 CDS 32774633 32774780 32774633 ID=cds.MS.gene77098.t1;Parent=MS.gene77098.t1
chr2.4 exon 32773982 32774271 32773982 ID=MS.gene77098.t1.exon4;Parent=MS.gene77098.t1
chr2.4 CDS 32773982 32774271 32773982 ID=cds.MS.gene77098.t1;Parent=MS.gene77098.t1
chr2.4 exon 32773285 32773571 32773285 ID=MS.gene77098.t1.exon5;Parent=MS.gene77098.t1
chr2.4 CDS 32773285 32773571 32773285 ID=cds.MS.gene77098.t1;Parent=MS.gene77098.t1
Gene Sequence

>MS.gene77098

AGGCATTTGATCCATTGGATCCAACTGGGAATGTGACAATAAGATGGGATATTATGTCATGGACATCAGATGGTTATATGGTAAGTAAATTCCAAACTTTGTCTGATCATGCTATTCTTTTAAGGAAAAGTTAACTACAAATGTAATCATTATAAGTTGTGCATACCAAAATGTTTCCAAAAATGATGAAATCACTGGTCTTTTGTGATTACCAAAAGAATAAAATTATCAAAATTCAAATATTATGAACTTATAATATATAATCTGATGCTGATGTCGAACTAAGAAATAGATTCGAGAAGCTAGAAAAGGATTTGAATAATGGTGGATTAATTGATACTCCTGATATAGTAACACATAATAATACTCTATATGATAGAAGTGTTTCATCTTAATATTCGCATAATAACACGGTGTAACACACATAGATGTTTGGATCCCTTAACCATAGGAGTTCGTGTCATGGCCAGTGCGTATCGAAAAACATATTTTCAAAATGGTGAATCCTTTAAATGAATCTCAGTTACTTCGAGGGGTTAATCTCCACAATTACACGTGGATGATAGCTTAATTTACACACAAAAATTCGCTAATTAATGCTCTAGCTATCTTCAAACTAATCAAGATACTATGATGTCAAATTTTGGCAGGCAACTGTAACTTTGTTTAACTTTCAACTATACCGAAACATTATGAACCCGGGATGGACATTAGGATGGACATGGGCCAAGAAAGAAATCATATGGGCCATGATGGGAGCGCAAGCAACAGAACAAGGAGATTGTTCAAAGTTCAAGTTAAAGATTCCTCATAGTTGCAAGAGAAGTCCTCAAGTAGTTGACTTATTACCTGGAGCATCTTACAATATGCAATACACAAACTGTTGCAAAGGTGGTGTGCTAACATCTTGGGGACAGGACCCTTCAGGTGCAATCTCGGCATTTCAGATGGGTGTAGGACTCTCCGGAAGGACTAACAAGACAGTTAAACTACCTCAGGATTTTAAATTGCTTGGACCGGGAGCAGGATATTCATGTGGTCCGGCTAAGAGAGTTCCTTCAACTGTTATTCTTACAGATGATCGTAGGCGAAAAGCTCAAGCTTTGAGTATGCATACTAATTCCCTTTGAGTATATTGCAAATAGTTATGGTTTACAATCACGATTTTAAATTGAATCTGCATCGTAATTTTTAATACTGCCGAGAATTATGGTCAAATGTAGCTGATGCAACCTCAGTCTCAGTCGTATTGCAGTCATTGTGACATCAAAACATGATGTCGCGACCTTGATTGTAATTGCAGATCCTTTTCGAAAACCTTGATGGTTACAATCAACGCTTTAAAGAAACTGTCCTGGAAATAGTATTATCTAATATTCGATAACAAAGTTTGTGATACAAACACAAAGCGACGATCACTACAGGTTAAAACCTTGAATACAAATGTACTATTAATATTATCTAATGTTCCTGTATTTTTTTCCTATGCAGTGTCCTGGAATGTGACATGTACTTATTCACAATTTCTTGCCTCCAAGAACCCAAGCTGTTGTGTTTCTTTATCAACATTCTACAATGACCAAGTCACAGCTTGTCCAACTTGTGCTTGTGGTTGTCAGAATAATGCTACTTGCGTAACGTAAGACGCCTTAATTTCTACTTCCACATTATTAGCCAACTTTTAAACTGCTGTCGAGGTTGCATCATAATCATTTATAGGACAAGAAATTCAGTCAAATACAATTGGTTGATGCAACTTCAATTGCAGTTGTAGAGACATCGTTTTGTGATGTCGCAGCGTAGATTACAAAAGCTTTCCGTGTCCAAAATCATGATTATTAGTCTTAACCATATGGCTGCAATAGCAGTTATAGAAACCTCTAAAACCTTTTATATTGTGGCCGCAGTTGCAGTCACAGGACGCAATTTAGAGCCTTATAAGTTATAACATTATTTGGAAATAGTAATGCTAATTTTGATTTGTCATTATGCAAATGCAGGAAAGATTCAAAGATCCTGAAAGAGGTTGCTGGAAATAACAAGACTAGGAAGAGTGATATCGCACCAAAACCTTTATTGCAATGCACACGCCACCTTTGTCCTGTTCGTGTGCATTGGCATATAAAAGATAATTATAAGGATTATTGGCGTGTAAAGATTGCAATCATCAACTTCAAGTATAGAATGAATTACACACAATGGGGTCTTGTTGTGCAACACCCTAATCTCAGTAATGTTACCCAAGTCTATAGTTTTGAATACATGCCACTTCTTCCTTATGAAGCCATAAGTAAGTTCTTCAAATATTAGACACCGTCTATATTTTGTTTACTTGAATTACAAAATGAAATTAACAGAACAAGAAAATGAATTTCTTATCTAACTTTGACGTCTCTAATACAAATTCGGATTGCATGCAGTCACAATTGTTGCATTGCAATCAACATGTTTGTGGTCATCCGATTAATATCGTACAATCCAAATTTCAAGCCAATTTATTTATTTTTTTAATCTAAAATACCAAGCTTGAAATCTGGACCGTCTGATCTTAATAAGACAACCACATATGTGCCACTGTATGAATGCGTGCAATTTCGAGTGCATACAATCCAAATACATATAATATCCCTATAATGCATAGAGTCACATGAAGAGTTATTGTTTTTTTAACTTTTGTGCATGGCTGAATTGTGTATGCAGATGACACAGGAATGTTCTATGGTTTGAAATTCTACAATGATTTAATAATGGAAGCTGGGGCTAAAGGGAATGTGCAATCTGAGGTGCTTATGAAGAAGGACAAGAACACATTTACACTAAAGCAAGGATGGGCTTTTCCTAGAAGGGTATACTTTAATGGTGATGAATGTATGCTTCCACCTCCTGATTCATACCCTTTCTTGCCAAATTCTGCTCAAAGGCTACCTACAAGTGTTGTAATGATGACAGCCTATGTGATTATTGCATCATTTTTCATTTGGTTTTGA
Protein sequence

>MS.gene77098.t1

RHLIHWIQLGMUQUDGILCHGHQMVIWQLULCLTFNYTETLUTRDGHUDGHGPRKKSYGPUWERKQQNKEIVQSSSURFLIVAREVLKULTYYLEHLTICNTQTVAKVVCUHLGDRTLQVQSRHFRWVUDSPEGLTRQLNYLRILNCLDREQDIHVVRLREFLQLLFLQMIVGEKLKLUCPGMUHVLIHNFLPPRTQAVVFLYQHSTMTKSQLVQLVLVVVRIMLLAURKIQRSUKRLLEITRLGRVISHQNLYCNAHATFVLFVCIGIUKIIIRIIGVURLQSSTSSIEUITHNGVLLCNTLISVMLPKSIVLNTCHFFLMKPUMTQECSMVUNSTMIUUWKLGLKGMCNLRCLURRTRTHLHUSKDGLFLEGYTLMVMNVCFHLLIHTLSCQILLKGYLQVLUUUQPMULLHHFSFGF