Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77130.t1 | KEH17810.1 | 42.7 | 117 | 24 | 4 | 1 | 75 | 1 | 116 | 1.60E-10 | 75.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77130.t1 | A0A072TWC6 | 42.7 | 117 | 24 | 4 | 1 | 75 | 1 | 116 | 1.1e-10 | 75.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050719 | MS.gene77130 | 0.806728 | 7.14E-50 | -1.69E-46 |
| MS.gene050721 | MS.gene77130 | 0.804974 | 1.67E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77130.t1 | MTR_8g006610 | 43.590 | 117 | 23 | 4 | 1 | 75 | 1 | 116 | 5.65e-20 | 77.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAATTTGAAGAAGAGGATT+TGG | 0.319326 | 2.4:+32416480 | MS.gene77130:CDS |
| GTTAACACATGGTATCGTTT+TGG | 0.351051 | 2.4:-32416454 | None:intergenic |
| AATATTCATGCAAACAATAA+AGG | 0.353795 | 2.4:+32418284 | MS.gene77130:CDS |
| TGAAGAAGAGGATTTGGTTA+TGG | 0.363993 | 2.4:+32416486 | MS.gene77130:CDS |
| TCATGTTCATCTAGACTATC+GGG | 0.429027 | 2.4:+32418063 | MS.gene77130:CDS |
| AACAATAAAGGACTTGATGC+TGG | 0.467302 | 2.4:+32418296 | MS.gene77130:CDS |
| AACTTATGAATTTGAAGAAG+AGG | 0.473125 | 2.4:+32416474 | MS.gene77130:CDS |
| TTCATGTTCATCTAGACTAT+CGG | 0.476826 | 2.4:+32418062 | MS.gene77130:CDS |
| AAAGTTTGAACTTACTGCCA+TGG | 0.508354 | 2.4:-32416520 | None:intergenic |
| AGTTCAGACGATATTGAGTA+AGG | 0.523623 | 2.4:+32418320 | MS.gene77130:CDS |
| AATATTGGAAATGAGATGGA+TGG | 0.552754 | 2.4:+32416424 | None:intergenic |
| CAAATTCATAAGTTAACACA+TGG | 0.591019 | 2.4:-32416465 | None:intergenic |
| TCATCTAGACTATCGGGGAA+AGG | 0.604734 | 2.4:+32418069 | MS.gene77130:CDS |
| TTATGGTTAGAGATAATCCA+TGG | 0.625033 | 2.4:+32416503 | MS.gene77130:CDS |
| CATGTTCATCTAGACTATCG+GGG | 0.680390 | 2.4:+32418064 | MS.gene77130:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTAATATTAATCATAAAGT+TGG | - | chr2.4:32416889-32416908 | None:intergenic | 10.0% |
| !! | ATTCTATACATTATGAAAAA+TGG | - | chr2.4:32416937-32416956 | None:intergenic | 15.0% |
| !!! | AAAAGCATTTATAAGTTTTT+AGG | - | chr2.4:32417054-32417073 | None:intergenic | 15.0% |
| !!! | ATTAGTTTATAGAAAACATA+TGG | - | chr2.4:32417840-32417859 | None:intergenic | 15.0% |
| !!! | TGTTTTCTATAAACTAATTA+TGG | + | chr2.4:32417842-32417861 | MS.gene77130:intron | 15.0% |
| !! | AATATTCATGCAAACAATAA+AGG | + | chr2.4:32418284-32418303 | MS.gene77130:CDS | 20.0% |
| !! | CTAGTTAATTAATTAGTGAA+TGG | + | chr2.4:32416718-32416737 | MS.gene77130:intron | 20.0% |
| !! | TATTAATCATAAAGTTGGAA+AGG | - | chr2.4:32416884-32416903 | None:intergenic | 20.0% |
| !! | TCTATAACTACATTTGAATA+TGG | + | chr2.4:32417256-32417275 | MS.gene77130:intron | 20.0% |
| !! | TCTATTACATTTCAATCTAT+GGG | - | chr2.4:32417127-32417146 | None:intergenic | 20.0% |
| !!! | AAAATGACATTTTAGTAATG+AGG | + | chr2.4:32418092-32418111 | MS.gene77130:CDS | 20.0% |
| !!! | CAAATATTCTTTGCTTTTAT+TGG | + | chr2.4:32416598-32416617 | MS.gene77130:intron | 20.0% |
| !!! | TTACTTATATTACTTTTCCT+TGG | + | chr2.4:32417704-32417723 | MS.gene77130:intron | 20.0% |
| !!! | TTGTTTTGTCTACTAATTTT+AGG | - | chr2.4:32417187-32417206 | None:intergenic | 20.0% |
| ! | AAAATCTGTTGTTCAATTTG+TGG | - | chr2.4:32417740-32417759 | None:intergenic | 25.0% |
| ! | AACTGCATTTCAAAAATTCT+TGG | + | chr2.4:32417677-32417696 | MS.gene77130:intron | 25.0% |
| ! | AACTTATGAATTTGAAGAAG+AGG | + | chr2.4:32416474-32416493 | MS.gene77130:CDS | 25.0% |
| ! | AATACTGACACAACTAAAAA+TGG | - | chr2.4:32418261-32418280 | None:intergenic | 25.0% |
| ! | AATGCATAAACTGATCAATT+TGG | + | chr2.4:32417936-32417955 | MS.gene77130:intron | 25.0% |
| ! | ATAACTACATTTGAATATGG+TGG | + | chr2.4:32417259-32417278 | MS.gene77130:intron | 25.0% |
| ! | CAAATTCATAAGTTAACACA+TGG | - | chr2.4:32416468-32416487 | None:intergenic | 25.0% |
| ! | CTATTACATTTCAATCTATG+GGG | - | chr2.4:32417126-32417145 | None:intergenic | 25.0% |
| ! | CTCTATTACATTTCAATCTA+TGG | - | chr2.4:32417128-32417147 | None:intergenic | 25.0% |
| ! | TGTGAGTAGTTTATTGATAA+TGG | - | chr2.4:32416986-32417005 | None:intergenic | 25.0% |
| !! | AGTGTCTTTTGATGTAATTT+TGG | + | chr2.4:32417372-32417391 | MS.gene77130:intron | 25.0% |
| !! | GTGTCTTTTGATGTAATTTT+GGG | + | chr2.4:32417373-32417392 | MS.gene77130:intron | 25.0% |
| !!! | CTAATTTTAGGTTGTTTAGT+TGG | - | chr2.4:32417175-32417194 | None:intergenic | 25.0% |
| ATCAGTTTATGCATTTCCAA+CGG | - | chr2.4:32417931-32417950 | None:intergenic | 30.0% | |
| CACACACTAAAGAAAACATA+CGG | - | chr2.4:32417892-32417911 | None:intergenic | 30.0% | |
| TGAATTTGAAGAAGAGGATT+TGG | + | chr2.4:32416480-32416499 | MS.gene77130:CDS | 30.0% | |
| TTATGGTTAGAGATAATCCA+TGG | + | chr2.4:32416503-32416522 | MS.gene77130:CDS | 30.0% | |
| TTCATGTTCATCTAGACTAT+CGG | + | chr2.4:32418062-32418081 | MS.gene77130:CDS | 30.0% | |
| ! | ACTACTCACAAACATTTTAG+AGG | + | chr2.4:32416996-32417015 | MS.gene77130:intron | 30.0% |
| ! | ATGTAATTTTGGGCTTATCA+TGG | + | chr2.4:32417383-32417402 | MS.gene77130:intron | 30.0% |
| ! | GCCTTTTGATAGAAAAAAGA+AGG | + | chr2.4:32417864-32417883 | MS.gene77130:intron | 30.0% |
| ! | TTTTCCACTTGTTCAATGAA+TGG | - | chr2.4:32417816-32417835 | None:intergenic | 30.0% |
| !! | AAGTGTGGTTTTTAATTAGC+TGG | + | chr2.4:32416674-32416693 | MS.gene77130:intron | 30.0% |
| !!! | ATCAGTTGACTTGTTTTTGT+GGG | + | chr2.4:32417503-32417522 | MS.gene77130:intron | 30.0% |
| !!! | GCCTTCTTTTTTCTATCAAA+AGG | - | chr2.4:32417868-32417887 | None:intergenic | 30.0% |
| AAAGTTTGAACTTACTGCCA+TGG | - | chr2.4:32416523-32416542 | None:intergenic | 35.0% | |
| AACAATAAAGGACTTGATGC+TGG | + | chr2.4:32418296-32418315 | MS.gene77130:CDS | 35.0% | |
| ACTGTGAATTAAATGACCGT+TGG | + | chr2.4:32417912-32417931 | MS.gene77130:intron | 35.0% | |
| AGAAAGGTTCCGTATATAAG+CGG | - | chr2.4:32417587-32417606 | None:intergenic | 35.0% | |
| CACAAAAACAAGTCAACTGA+TGG | - | chr2.4:32417504-32417523 | None:intergenic | 35.0% | |
| CTAGAGAAAAGCTTAGATCA+GGG | - | chr2.4:32417024-32417043 | None:intergenic | 35.0% | |
| GACACAACTAAAAATGGTAG+AGG | - | chr2.4:32418255-32418274 | None:intergenic | 35.0% | |
| GTAACCTTTCTTGTTCCAAA+AGG | + | chr2.4:32416857-32416876 | MS.gene77130:intron | 35.0% | |
| TCATGTTCATCTAGACTATC+GGG | + | chr2.4:32418063-32418082 | MS.gene77130:CDS | 35.0% | |
| TCTAGAGAAAAGCTTAGATC+AGG | - | chr2.4:32417025-32417044 | None:intergenic | 35.0% | |
| TGTAGGAGTTGAATGTTAGT+GGG | + | chr2.4:32417538-32417557 | MS.gene77130:intron | 35.0% | |
| TGTGAAAAATGACGGTTAAG+TGG | + | chr2.4:32418154-32418173 | MS.gene77130:intron | 35.0% | |
| TTGTAGGAGTTGAATGTTAG+TGG | + | chr2.4:32417537-32417556 | MS.gene77130:intron | 35.0% | |
| TTTGTGGACTTATGTTACCA+AGG | - | chr2.4:32417724-32417743 | None:intergenic | 35.0% | |
| ! | AAGAAGGGTGTGAAAAATGA+CGG | + | chr2.4:32418146-32418165 | MS.gene77130:intron | 35.0% |
| ! | ACCATTAGCTTGAATCATCT+TGG | + | chr2.4:32416810-32416829 | MS.gene77130:intron | 35.0% |
| ! | ATTTATTGTCGTGGCTGTTT+TGG | + | chr2.4:32417465-32417484 | MS.gene77130:intron | 35.0% |
| ! | CCATTAGCTTGAATCATCTT+GGG | + | chr2.4:32416811-32416830 | MS.gene77130:intron | 35.0% |
| ! | GTTAACACATGGTATCGTTT+TGG | - | chr2.4:32416457-32416476 | None:intergenic | 35.0% |
| ! | GTTGTTGTTCTTTAGAGACA+GGG | - | chr2.4:32416549-32416568 | None:intergenic | 35.0% |
| ! | TAAAGTTGGAAAGGTCCTTT+TGG | - | chr2.4:32416875-32416894 | None:intergenic | 35.0% |
| ! | TAATTTTGGGCTTATCATGG+TGG | + | chr2.4:32417386-32417405 | MS.gene77130:intron | 35.0% |
| ! | TGAAGAAGAGGATTTGGTTA+TGG | + | chr2.4:32416486-32416505 | MS.gene77130:CDS | 35.0% |
| ! | TGTTGTTGTTCTTTAGAGAC+AGG | - | chr2.4:32416550-32416569 | None:intergenic | 35.0% |
| !! | ATACTTTTGATTCTCCTGTG+TGG | + | chr2.4:32417770-32417789 | MS.gene77130:intron | 35.0% |
| !!! | CATCAGTTGACTTGTTTTTG+TGG | + | chr2.4:32417502-32417521 | MS.gene77130:intron | 35.0% |
| AAATGACGGTTAAGTGGTGT+TGG | + | chr2.4:32418160-32418179 | MS.gene77130:intron | 40.0% | |
| ACCAAATCAAGGTGATGTCT+TGG | + | chr2.4:32417282-32417301 | MS.gene77130:intron | 40.0% | |
| ACCAACTTTGCGAAATGGAT+AGG | + | chr2.4:32418207-32418226 | MS.gene77130:intron | 40.0% | |
| ACCAAGACATCACCTTGATT+TGG | - | chr2.4:32417286-32417305 | None:intergenic | 40.0% | |
| ATCAGTTGCATGCTATTACG+TGG | - | chr2.4:32416772-32416791 | None:intergenic | 40.0% | |
| CAAGCTGCATAGATAGAGAA+AGG | - | chr2.4:32417603-32417622 | None:intergenic | 40.0% | |
| CATGTTCATCTAGACTATCG+GGG | + | chr2.4:32418064-32418083 | MS.gene77130:CDS | 40.0% | |
| CCCAAGATGATTCAAGCTAA+TGG | - | chr2.4:32416814-32416833 | None:intergenic | 40.0% | |
| CGTCTGTAGCCAATGTTATA+GGG | - | chr2.4:32418187-32418206 | None:intergenic | 40.0% | |
| CTTCTTGCCTTCACTATGAA+TGG | + | chr2.4:32417333-32417352 | MS.gene77130:intron | 40.0% | |
| GAGGTATGTCCGCTTATATA+CGG | + | chr2.4:32417575-32417594 | MS.gene77130:intron | 40.0% | |
| GCCACGACAATAAATCACAA+CGG | - | chr2.4:32417460-32417479 | None:intergenic | 40.0% | |
| GCTACCATTCATTGAACAAG+TGG | + | chr2.4:32417809-32417828 | MS.gene77130:intron | 40.0% | |
| GTAGGAGTTGAATGTTAGTG+GGG | + | chr2.4:32417539-32417558 | MS.gene77130:intron | 40.0% | |
| TAGGAGTTGAATGTTAGTGG+GGG | + | chr2.4:32417540-32417559 | MS.gene77130:intron | 40.0% | |
| TCAAGGTGATGTCTTGGTAT+TGG | + | chr2.4:32417288-32417307 | MS.gene77130:intron | 40.0% | |
| TCCAACACCATTCATAGTGA+AGG | - | chr2.4:32417343-32417362 | None:intergenic | 40.0% | |
| TCGTCTGTAGCCAATGTTAT+AGG | - | chr2.4:32418188-32418207 | None:intergenic | 40.0% | |
| TTTCCTTTATGTCCAGCATG+AGG | + | chr2.4:32418032-32418051 | MS.gene77130:intron | 40.0% | |
| TTTGCGAAATGGATAGGCTA+CGG | + | chr2.4:32418213-32418232 | MS.gene77130:intron | 40.0% | |
| ! | ATGAATGGTGTTGGAACTCA+AGG | + | chr2.4:32417348-32417367 | MS.gene77130:intron | 40.0% |
| ! | TCATCTTGGGCATGTGTTTT+TGG | + | chr2.4:32416824-32416843 | MS.gene77130:intron | 40.0% |
| ! | TGAATGGTGTTGGAACTCAA+GGG | + | chr2.4:32417349-32417368 | MS.gene77130:intron | 40.0% |
| !! | GAATATGGTGGACCAAATCA+AGG | + | chr2.4:32417271-32417290 | MS.gene77130:intron | 40.0% |
| !! | GTGGGATTTTGCTAGTTTGT+AGG | + | chr2.4:32417521-32417540 | MS.gene77130:intron | 40.0% |
| !!! | AGGTCCTTTTGGAACAAGAA+AGG | - | chr2.4:32416864-32416883 | None:intergenic | 40.0% |
| !!! | GCTGTTTTGGTTCTATCACA+GGG | + | chr2.4:32417478-32417497 | MS.gene77130:intron | 40.0% |
| !!! | GTAGCTGTTGTTTTGCTGAT+TGG | + | chr2.4:32417415-32417434 | MS.gene77130:intron | 40.0% |
| ACATTCTCCTCAGGGAAGAA+GGG | + | chr2.4:32418131-32418150 | MS.gene77130:intron | 45.0% | |
| ACTCCTCATGCTGGACATAA+AGG | - | chr2.4:32418038-32418057 | None:intergenic | 45.0% | |
| AGACGACCAACTTTGCGAAA+TGG | + | chr2.4:32418202-32418221 | MS.gene77130:intron | 45.0% | |
| ATGAACTTGACTCCTCATGC+TGG | - | chr2.4:32418047-32418066 | None:intergenic | 45.0% | |
| GAATGTTAGTGGGGGTTCTT+TGG | + | chr2.4:32417548-32417567 | MS.gene77130:intron | 45.0% | |
| GACTTTCATCTCAACCACAC+AGG | - | chr2.4:32417787-32417806 | None:intergenic | 45.0% | |
| GCCGTTGTGATTTATTGTCG+TGG | + | chr2.4:32417456-32417475 | MS.gene77130:intron | 45.0% | |
| GCCTATCCATTTCGCAAAGT+TGG | - | chr2.4:32418211-32418230 | None:intergenic | 45.0% | |
| GCCTTCACTATGAATGGTGT+TGG | + | chr2.4:32417339-32417358 | MS.gene77130:intron | 45.0% | |
| TAGAGCGAGACTCAAAAGTG+TGG | + | chr2.4:32416659-32416678 | MS.gene77130:intron | 45.0% | |
| TCATCTAGACTATCGGGGAA+AGG | + | chr2.4:32418069-32418088 | MS.gene77130:CDS | 45.0% | |
| ! | GGTGTTGGACCCTATAACAT+TGG | + | chr2.4:32418175-32418194 | MS.gene77130:intron | 45.0% |
| ! | TTTGGGCTTATCATGGTGGA+GGG | + | chr2.4:32417390-32417409 | MS.gene77130:intron | 45.0% |
| ! | TTTTGGGCTTATCATGGTGG+AGG | + | chr2.4:32417389-32417408 | MS.gene77130:intron | 45.0% |
| !! | CTCTAGAGAGCATCTAACAG+TGG | - | chr2.4:32416644-32416663 | None:intergenic | 45.0% |
| !!! | GGCTGTTTTGGTTCTATCAC+AGG | + | chr2.4:32417477-32417496 | MS.gene77130:intron | 45.0% |
| GACATTCTCCTCAGGGAAGA+AGG | + | chr2.4:32418130-32418149 | MS.gene77130:intron | 50.0% | |
| GCAGCTAGACATTCTCCTCA+GGG | + | chr2.4:32418123-32418142 | MS.gene77130:intron | 50.0% | |
| TGCAGCTAGACATTCTCCTC+AGG | + | chr2.4:32418122-32418141 | MS.gene77130:intron | 50.0% | |
| TTTCACACCCTTCTTCCCTG+AGG | - | chr2.4:32418141-32418160 | None:intergenic | 50.0% | |
| ! | GTGGGGGTTCTTTGGTTTCG+AGG | + | chr2.4:32417556-32417575 | MS.gene77130:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 32416439 | 32418340 | 32416439 | ID=MS.gene77130 |
| chr2.4 | mRNA | 32416439 | 32418340 | 32416439 | ID=MS.gene77130.t1;Parent=MS.gene77130 |
| chr2.4 | exon | 32416439 | 32416527 | 32416439 | ID=MS.gene77130.t1.exon1;Parent=MS.gene77130.t1 |
| chr2.4 | CDS | 32416439 | 32416527 | 32416439 | ID=cds.MS.gene77130.t1;Parent=MS.gene77130.t1 |
| chr2.4 | exon | 32418048 | 32418113 | 32418048 | ID=MS.gene77130.t1.exon2;Parent=MS.gene77130.t1 |
| chr2.4 | CDS | 32418048 | 32418113 | 32418048 | ID=cds.MS.gene77130.t1;Parent=MS.gene77130.t1 |
| chr2.4 | exon | 32418268 | 32418340 | 32418268 | ID=MS.gene77130.t1.exon3;Parent=MS.gene77130.t1 |
| chr2.4 | CDS | 32418268 | 32418340 | 32418268 | ID=cds.MS.gene77130.t1;Parent=MS.gene77130.t1 |
| Gene Sequence |
| Protein sequence |
