AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77192

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77192.t1 MTR_2g062340 95.429 350 16 0 1 350 168 517 0.0 706
MS.gene77192.t1 MTR_2g067640 53.125 384 138 6 1 349 164 540 1.38e-136 400
MS.gene77192.t1 MTR_2g062430 53.230 387 132 9 1 349 177 552 1.76e-133 392
MS.gene77192.t1 MTR_4g131710 50.886 395 140 8 1 349 199 585 9.49e-131 387
MS.gene77192.t1 MTR_2g062410 52.174 391 135 9 1 349 205 585 3.58e-129 383
MS.gene77192.t1 MTR_2g062370 51.392 395 136 9 1 349 205 589 7.63e-126 374
MS.gene77192.t1 MTR_2g062380 50.253 396 139 10 1 349 175 559 1.99e-122 365
MS.gene77192.t1 MTR_4g077620 47.959 392 156 7 2 351 184 569 4.11e-118 354
MS.gene77192.t1 MTR_1g080370 46.272 389 163 6 2 349 175 558 2.34e-113 341
MS.gene77192.t1 MTR_1g080410 46.272 389 163 6 2 349 175 558 2.34e-113 341
MS.gene77192.t1 MTR_5g007860 46.565 393 162 7 2 351 220 607 5.83e-113 342
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77192.t1 AT2G44260 51.031 388 140 7 2 349 175 552 6.32e-131 386
MS.gene77192.t1 AT2G44260 51.031 388 140 7 2 349 205 582 1.55e-130 386
MS.gene77192.t1 AT2G44230 47.165 388 152 8 2 349 167 541 4.38e-115 345
MS.gene77192.t1 AT3G01880 46.410 390 157 8 2 351 214 591 2.14e-112 340
MS.gene77192.t1 AT3G01870 46.907 388 152 9 2 349 210 583 3.47e-112 339
MS.gene77192.t1 AT3G04350 45.729 398 160 10 2 349 152 543 4.11e-110 333
MS.gene77192.t1 AT3G04350 45.729 398 160 10 2 349 174 565 1.60e-109 332
MS.gene77192.t1 AT1G04090 45.340 397 164 9 2 350 180 571 2.21e-107 327
MS.gene77192.t1 AT5G18490 44.872 390 164 9 2 349 171 551 3.84e-106 323
MS.gene77192.t1 AT5G43950 45.202 396 168 9 2 351 174 566 3.37e-105 321

Find 94 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAGTTGCTGATGAATATT+TGG 0.200710 2.4:+31536598 MS.gene77192:CDS
GTCTCAATTTGGTGTAAATT+AGG 0.204765 2.4:-31535909 None:intergenic
CCTTGATTAATGTCTCAATT+TGG 0.260601 2.4:-31535920 None:intergenic
GCAACTTTAGTGGAGAATTA+TGG 0.266412 2.4:+31536363 MS.gene77192:CDS
GTGTGAGAGGGTTTGGTATA+AGG 0.271860 2.4:+31536525 MS.gene77192:CDS
ACTGGGAGCATGTGACATTA+AGG 0.286859 2.4:+31536336 MS.gene77192:CDS
GGCTTAACTATGAGATGAAT+TGG 0.291359 2.4:+31536645 MS.gene77192:CDS
GATGGACCACTTGTGATTAT+TGG 0.313962 2.4:+31536900 MS.gene77192:CDS
GCTTAACTATGAGATGAATT+GGG 0.331254 2.4:+31536646 MS.gene77192:CDS
TTTGATCCCGTTGGTTCAAT+TGG 0.353977 2.4:-31536062 None:intergenic
ATGGTCATGCCTTGTTTCCT+AGG 0.354059 2.4:+31536483 MS.gene77192:CDS
GGACCTTTAGGCCCTTCCTT+TGG 0.361312 2.4:-31536671 None:intergenic
GCTTCAACACCTCTCTTGAT+TGG 0.379642 2.4:-31535777 None:intergenic
CATGCCTTGTTTCCTAGGCC+AGG 0.379884 2.4:+31536488 MS.gene77192:CDS
AAGGTGAACATGTGGGAGAC+TGG 0.402645 2.4:+31536318 MS.gene77192:CDS
CCATAGGCATGTCTAGCTAT+TGG 0.409330 2.4:+31536105 MS.gene77192:CDS
AATACAAGTCAATGAAGCTT+TGG 0.413133 2.4:+31535715 MS.gene77192:CDS
TTGGTGATGGATATGGTAAA+AGG 0.415224 2.4:+31536569 MS.gene77192:CDS
ATAGTTGCTGATGAATATTT+GGG 0.419807 2.4:+31536599 MS.gene77192:CDS
ATACATGTATTTGCATCCTA+TGG 0.424614 2.4:+31535953 MS.gene77192:CDS
ATGCAAGGTGTGAGAGGGTT+TGG 0.437474 2.4:+31536518 MS.gene77192:CDS
GAGTGATTTGGTGATGGATA+TGG 0.448214 2.4:+31536562 MS.gene77192:CDS
CTATTGGTTGGACCTGCCTA+TGG 0.449825 2.4:+31536121 MS.gene77192:CDS
GTGGTTTATTCTTCATTGCA+TGG 0.451048 2.4:+31536464 MS.gene77192:CDS
TGTTCACCTTTACTCCTCAA+TGG 0.452360 2.4:-31536305 None:intergenic
ACCTTCCATCTTCTGTGGAA+TGG 0.456713 2.4:+31535982 MS.gene77192:CDS
TCTTTCTCAGCTTCATCCAT+AGG 0.466572 2.4:-31536137 None:intergenic
CACAACATAGCAAGGGTCAA+TGG 0.469105 2.4:+31536399 MS.gene77192:CDS
ATGCCTTGTTTCCTAGGCCA+GGG 0.470358 2.4:+31536489 MS.gene77192:CDS
GAAGCTAAAGGTGTTTATGT+AGG 0.472275 2.4:+31535795 MS.gene77192:CDS
CCTTCCACATTGAGCAAGGA+AGG 0.480715 2.4:-31535820 None:intergenic
TGATGCATGTAAGAGTGATT+TGG 0.481205 2.4:+31536550 MS.gene77192:CDS
CCAAATTGAGACATTAATCA+AGG 0.482303 2.4:+31535920 MS.gene77192:CDS
TTAAGGGTAAGCAACTTTAG+TGG 0.484513 2.4:+31536353 MS.gene77192:CDS
TCTCAGCTTCATCCATAGGC+AGG 0.486648 2.4:-31536133 None:intergenic
ATAGGATGCAAATACATGTA+TGG 0.489650 2.4:-31535951 None:intergenic
GCCTAAAGGTCCAAAACAAA+AGG 0.493717 2.4:+31536682 MS.gene77192:CDS
ACAACATAGCAAGGGTCAAT+GGG 0.498048 2.4:+31536400 MS.gene77192:CDS
TGGTGATGGATATGGTAAAA+GGG 0.499644 2.4:+31536570 MS.gene77192:CDS
GTGAAAGTTCCTCCTAGCAT+TGG 0.500389 2.4:-31536212 None:intergenic
TAATCACAAGTGGTCCATCA+TGG 0.503360 2.4:-31536896 None:intergenic
AACATTCCATTGAGGAGTAA+AGG 0.504776 2.4:+31536299 MS.gene77192:CDS
CTGGGAGCATGTGACATTAA+GGG 0.510466 2.4:+31536337 MS.gene77192:CDS
GCGAGTCCAATTGAACCAAC+GGG 0.520978 2.4:+31536056 MS.gene77192:CDS
AATGTAAAACTTGCCCATGA+TGG 0.521062 2.4:+31536882 MS.gene77192:CDS
TGAGGAAGGTTTGATCCCGT+TGG 0.522959 2.4:-31536071 None:intergenic
AGGTGAACATGTGGGAGACT+GGG 0.530252 2.4:+31536319 MS.gene77192:CDS
GGAAGAAAATCTATTAGCAT+TGG 0.530712 2.4:-31535883 None:intergenic
CCAATAGCTAGACATGCCTA+TGG 0.535104 2.4:-31536105 None:intergenic
CAGAAATTAGAGAGCCACCT+TGG 0.537553 2.4:+31536624 MS.gene77192:CDS
ATGAATTGGGGTCCAAAGGA+AGG 0.538334 2.4:+31536659 MS.gene77192:CDS
AAGAGAGGTGTTGAAGCTAA+AGG 0.538834 2.4:+31535783 MS.gene77192:CDS
CCTCAAGGATGTTCATCCAT+AGG 0.544941 2.4:+31536089 MS.gene77192:CDS
GGTCCAAAGGAAGGGCCTAA+AGG 0.548515 2.4:+31536668 MS.gene77192:CDS
GTGTTATGCAAGGTGTGAGA+GGG 0.549325 2.4:+31536513 MS.gene77192:CDS
CCTTCCTTGCTCAATGTGGA+AGG 0.549333 2.4:+31535820 MS.gene77192:CDS
AGGCATGTCTAGCTATTGGT+TGG 0.560671 2.4:+31536109 MS.gene77192:CDS
TGCGAGTCCAATTGAACCAA+CGG 0.560820 2.4:+31536055 MS.gene77192:CDS
CTTTCACTGATTTAGTCATG+TGG 0.565265 2.4:+31536228 MS.gene77192:CDS
TGAGATGAATTGGGGTCCAA+AGG 0.567740 2.4:+31536655 MS.gene77192:CDS
TGCATAACACACCCTGGCCT+AGG 0.569608 2.4:-31536500 None:intergenic
GTTCATGTAAAACCAATGCT+AGG 0.571428 2.4:+31536200 MS.gene77192:CDS
AGGAGTAAAGGTGAACATGT+GGG 0.572262 2.4:+31536311 MS.gene77192:CDS
AATCACAAGTGGTCCATCAT+GGG 0.573305 2.4:-31536895 None:intergenic
TTTCACTGATTTAGTCATGT+GGG 0.574054 2.4:+31536229 MS.gene77192:CDS
ATATTTGTCACAACATAGCA+AGG 0.576601 2.4:+31536391 MS.gene77192:CDS
AGGCCAGGGTGTGTTATGCA+AGG 0.581565 2.4:+31536503 MS.gene77192:CDS
CTTAACTATGAGATGAATTG+GGG 0.582890 2.4:+31536647 MS.gene77192:CDS
GATGGAAGGTACTTATCCAT+AGG 0.584807 2.4:-31535969 None:intergenic
ACACCCTGGCCTAGGAAACA+AGG 0.586907 2.4:-31536492 None:intergenic
TGGATGAACATCCTTGAGGA+AGG 0.591376 2.4:-31536085 None:intergenic
ATCTAGCCAATAATCACAAG+TGG 0.592072 2.4:-31536906 None:intergenic
TAAGTACCTTCCATCTTCTG+TGG 0.594810 2.4:+31535977 MS.gene77192:CDS
TTTGGAGAACAGAAAACAAG+AGG 0.597580 2.4:+31535733 MS.gene77192:CDS
AATTTGGTGTAAATTAGGCA+TGG 0.598258 2.4:-31535904 None:intergenic
ACCATTCCACAGAAGATGGA+AGG 0.601642 2.4:-31535983 None:intergenic
TGAATTGGGGTCCAAAGGAA+GGG 0.606293 2.4:+31536660 MS.gene77192:CDS
CATTCATGAACATTCCATTG+AGG 0.607280 2.4:+31536291 MS.gene77192:CDS
TTCATCTCATAGTTAAGCCA+AGG 0.607470 2.4:-31536641 None:intergenic
GAGGAGTAAAGGTGAACATG+TGG 0.620013 2.4:+31536310 MS.gene77192:CDS
TGTGTTATGCAAGGTGTGAG+AGG 0.624086 2.4:+31536512 MS.gene77192:CDS
CATGTAAAACCAATGCTAGG+AGG 0.629716 2.4:+31536203 MS.gene77192:CDS
TGCAATGAAGAATAAACCAC+AGG 0.637004 2.4:-31536461 None:intergenic
ACGGGATCAAACCTTCCTCA+AGG 0.640922 2.4:+31536074 MS.gene77192:CDS
GGAACCTTCCTTGCTCAATG+TGG 0.644193 2.4:+31535816 MS.gene77192:CDS
CTTTAGTGGAGAATTATGGA+AGG 0.655206 2.4:+31536367 MS.gene77192:CDS
ACACCTTGCATAACACACCC+TGG 0.664930 2.4:-31536506 None:intergenic
ATGTAAGAGTGATTTGGTGA+TGG 0.666801 2.4:+31536556 MS.gene77192:CDS
ATTTGATACATTCATCAGTG+AGG 0.666824 2.4:-31535695 None:intergenic
GATGTTAGACCAATCAAGAG+AGG 0.668053 2.4:+31535768 MS.gene77192:CDS
ATCTCATAGTTAAGCCAAGG+TGG 0.674603 2.4:-31536638 None:intergenic
TATTTGTCACAACATAGCAA+GGG 0.678206 2.4:+31536392 MS.gene77192:CDS
TAGTTGCTGATGAATATTTG+GGG 0.691166 2.4:+31536600 MS.gene77192:CDS
CCTATGGATGAACATCCTTG+AGG 0.695842 2.4:-31536089 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTCTCTTTTTTCTTT+TGG + chr2.4:31536828-31536847 MS.gene77192:intron 15.0%
!!! TTTCTTTTGGTATAAATTTT+AGG + chr2.4:31536841-31536860 MS.gene77192:intron 15.0%
!! ATACCATAAGTATAAACAAT+AGG - chr2.4:31536808-31536827 None:intergenic 20.0%
!! TACCATAAGTATAAACAATA+GGG - chr2.4:31536807-31536826 None:intergenic 20.0%
!!! GTATTTTTCAAACAAACAAT+AGG - chr2.4:31535864-31535883 None:intergenic 20.0%
!!! TATTTCCATTTTTAAACTCA+AGG - chr2.4:31536439-31536458 None:intergenic 20.0%
! AATAGTTGCTGATGAATATT+TGG + chr2.4:31536598-31536617 MS.gene77192:CDS 25.0%
! ATAGTTGCTGATGAATATTT+GGG + chr2.4:31536599-31536618 MS.gene77192:CDS 25.0%
! TACCCTATTGTTTATACTTA+TGG + chr2.4:31536802-31536821 MS.gene77192:intron 25.0%
! TAGGGTATACAAAAACATTA+TGG - chr2.4:31536789-31536808 None:intergenic 25.0%
! TTCTCAATATTCGTCTTAAT+TGG + chr2.4:31536750-31536769 MS.gene77192:intron 25.0%
!! CTTTTGGTATAAATTTTAGG+TGG + chr2.4:31536844-31536863 MS.gene77192:intron 25.0%
!! TGGAATGGTTTTTCATAAAT+GGG + chr2.4:31535997-31536016 MS.gene77192:CDS 25.0%
!! TTTTACATGAACATAAGCTT+TGG - chr2.4:31536192-31536211 None:intergenic 25.0%
!!! AAACAAAAGGATTTTTGGAA+AGG + chr2.4:31536695-31536714 MS.gene77192:CDS 25.0%
!!! TTCCAAAAATCCTTTTGTTT+TGG - chr2.4:31536695-31536714 None:intergenic 25.0%
AATACAAGTCAATGAAGCTT+TGG + chr2.4:31535715-31535734 MS.gene77192:CDS 30.0%
ATACATGTATTTGCATCCTA+TGG + chr2.4:31535953-31535972 MS.gene77192:CDS 30.0%
ATACTCTACCAAAAAAGAGA+TGG + chr2.4:31536023-31536042 MS.gene77192:CDS 30.0%
ATAGGATGCAAATACATGTA+TGG - chr2.4:31535954-31535973 None:intergenic 30.0%
ATATTTGTCACAACATAGCA+AGG + chr2.4:31536391-31536410 MS.gene77192:CDS 30.0%
ATTTGATACATTCATCAGTG+AGG - chr2.4:31535698-31535717 None:intergenic 30.0%
CCAAATTGAGACATTAATCA+AGG + chr2.4:31535920-31535939 MS.gene77192:CDS 30.0%
CCTTGATTAATGTCTCAATT+TGG - chr2.4:31535923-31535942 None:intergenic 30.0%
CTTAACTATGAGATGAATTG+GGG + chr2.4:31536647-31536666 MS.gene77192:CDS 30.0%
GAGAAAGAAAGAGTCAAAAA+AGG + chr2.4:31536152-31536171 MS.gene77192:CDS 30.0%
GCTTAACTATGAGATGAATT+GGG + chr2.4:31536646-31536665 MS.gene77192:CDS 30.0%
GGAAGAAAATCTATTAGCAT+TGG - chr2.4:31535886-31535905 None:intergenic 30.0%
TAAAAATGGAAATAGACCTG+TGG + chr2.4:31536445-31536464 MS.gene77192:CDS 30.0%
TAGTTGCTGATGAATATTTG+GGG + chr2.4:31536600-31536619 MS.gene77192:CDS 30.0%
TATTTGTCACAACATAGCAA+GGG + chr2.4:31536392-31536411 MS.gene77192:CDS 30.0%
TGTGACCTTGAGTTTAAAAA+TGG + chr2.4:31536431-31536450 MS.gene77192:CDS 30.0%
! GGAATGGTTTTTCATAAATG+GGG + chr2.4:31535998-31536017 MS.gene77192:CDS 30.0%
! GGGTTTTCTATCCATTTAAT+GGG + chr2.4:31536249-31536268 MS.gene77192:CDS 30.0%
! GGTTTTCTATCCATTTAATG+GGG + chr2.4:31536250-31536269 MS.gene77192:CDS 30.0%
! GTCCAAAACAAAAGGATTTT+TGG + chr2.4:31536690-31536709 MS.gene77192:CDS 30.0%
! GTGGAATGGTTTTTCATAAA+TGG + chr2.4:31535996-31536015 MS.gene77192:CDS 30.0%
! GTTTTCTATCCATTTAATGG+GGG + chr2.4:31536251-31536270 MS.gene77192:CDS 30.0%
! TGGGTTTTCTATCCATTTAA+TGG + chr2.4:31536248-31536267 MS.gene77192:CDS 30.0%
!! AATTTGGTGTAAATTAGGCA+TGG - chr2.4:31535907-31535926 None:intergenic 30.0%
!! ATTTTAGGTGGCATTTAACA+TGG + chr2.4:31536856-31536875 MS.gene77192:intron 30.0%
!! ATTTTTGGAAAGGAGATGAA+CGG + chr2.4:31536705-31536724 MS.gene77192:CDS 30.0%
!! GTCTCAATTTGGTGTAAATT+AGG - chr2.4:31535912-31535931 None:intergenic 30.0%
!! TTTCACTGATTTAGTCATGT+GGG + chr2.4:31536229-31536248 MS.gene77192:CDS 30.0%
AAAAACCATTCCACAGAAGA+TGG - chr2.4:31535990-31536009 None:intergenic 35.0%
AACATTCCATTGAGGAGTAA+AGG + chr2.4:31536299-31536318 MS.gene77192:CDS 35.0%
AATGTAAAACTTGCCCATGA+TGG + chr2.4:31536882-31536901 MS.gene77192:CDS 35.0%
CATTCATGAACATTCCATTG+AGG + chr2.4:31536291-31536310 MS.gene77192:CDS 35.0%
CTTTAGTGGAGAATTATGGA+AGG + chr2.4:31536367-31536386 MS.gene77192:CDS 35.0%
GCAACTTTAGTGGAGAATTA+TGG + chr2.4:31536363-31536382 MS.gene77192:CDS 35.0%
GGCTTAACTATGAGATGAAT+TGG + chr2.4:31536645-31536664 MS.gene77192:CDS 35.0%
GTGGTTTATTCTTCATTGCA+TGG + chr2.4:31536464-31536483 MS.gene77192:CDS 35.0%
GTTCATGTAAAACCAATGCT+AGG + chr2.4:31536200-31536219 MS.gene77192:CDS 35.0%
TGATGCATGTAAGAGTGATT+TGG + chr2.4:31536550-31536569 MS.gene77192:CDS 35.0%
TGCAATGAAGAATAAACCAC+AGG - chr2.4:31536464-31536483 None:intergenic 35.0%
TTAAGGGTAAGCAACTTTAG+TGG + chr2.4:31536353-31536372 MS.gene77192:CDS 35.0%
TTCATCTCATAGTTAAGCCA+AGG - chr2.4:31536644-31536663 None:intergenic 35.0%
TTTGGAGAACAGAAAACAAG+AGG + chr2.4:31535733-31535752 MS.gene77192:CDS 35.0%
! ATCATGGGCAAGTTTTACAT+TGG - chr2.4:31536883-31536902 None:intergenic 35.0%
!! ATGTAAGAGTGATTTGGTGA+TGG + chr2.4:31536556-31536575 MS.gene77192:CDS 35.0%
!! CTTTCACTGATTTAGTCATG+TGG + chr2.4:31536228-31536247 MS.gene77192:CDS 35.0%
!! GAAGCTAAAGGTGTTTATGT+AGG + chr2.4:31535795-31535814 MS.gene77192:CDS 35.0%
!! TGGTGATGGATATGGTAAAA+GGG + chr2.4:31536570-31536589 MS.gene77192:CDS 35.0%
!! TTGGTGATGGATATGGTAAA+AGG + chr2.4:31536569-31536588 MS.gene77192:CDS 35.0%
!!! TAACGACACCATCTCTTTTT+TGG - chr2.4:31536034-31536053 None:intergenic 35.0%
!!! TCCTTTTGTTTTGGACCTTT+AGG - chr2.4:31536686-31536705 None:intergenic 35.0%
AATCACAAGTGGTCCATCAT+GGG - chr2.4:31536898-31536917 None:intergenic 40.0%
ACAACATAGCAAGGGTCAAT+GGG + chr2.4:31536400-31536419 MS.gene77192:CDS 40.0%
AGGAGTAAAGGTGAACATGT+GGG + chr2.4:31536311-31536330 MS.gene77192:CDS 40.0%
ATCTCATAGTTAAGCCAAGG+TGG - chr2.4:31536641-31536660 None:intergenic 40.0%
CATGTAAAACCAATGCTAGG+AGG + chr2.4:31536203-31536222 MS.gene77192:CDS 40.0%
GATGGACCACTTGTGATTAT+TGG + chr2.4:31536900-31536919 MS.gene77192:CDS 40.0%
GATGTTAGACCAATCAAGAG+AGG + chr2.4:31535768-31535787 MS.gene77192:CDS 40.0%
GCCTAAAGGTCCAAAACAAA+AGG + chr2.4:31536682-31536701 MS.gene77192:CDS 40.0%
TAAGTACCTTCCATCTTCTG+TGG + chr2.4:31535977-31535996 MS.gene77192:CDS 40.0%
TAATCACAAGTGGTCCATCA+TGG - chr2.4:31536899-31536918 None:intergenic 40.0%
TCTTTCTCAGCTTCATCCAT+AGG - chr2.4:31536140-31536159 None:intergenic 40.0%
TGTTCACCTTTACTCCTCAA+TGG - chr2.4:31536308-31536327 None:intergenic 40.0%
! TTTTCCTTCCACATTGAGCA+AGG - chr2.4:31535827-31535846 None:intergenic 40.0%
!! AAGAGAGGTGTTGAAGCTAA+AGG + chr2.4:31535783-31535802 MS.gene77192:CDS 40.0%
!! GAGTGATTTGGTGATGGATA+TGG + chr2.4:31536562-31536581 MS.gene77192:CDS 40.0%
!! GATGGAAGGTACTTATCCAT+AGG - chr2.4:31535972-31535991 None:intergenic 40.0%
!! TTTGATCCCGTTGGTTCAAT+TGG - chr2.4:31536065-31536084 None:intergenic 40.0%
ACCATTCCACAGAAGATGGA+AGG - chr2.4:31535986-31536005 None:intergenic 45.0%
ACCTTCCATCTTCTGTGGAA+TGG + chr2.4:31535982-31536001 MS.gene77192:CDS 45.0%
AGGCATGTCTAGCTATTGGT+TGG + chr2.4:31536109-31536128 MS.gene77192:CDS 45.0%
ATGAATTGGGGTCCAAAGGA+AGG + chr2.4:31536659-31536678 MS.gene77192:CDS 45.0%
CACAACATAGCAAGGGTCAA+TGG + chr2.4:31536399-31536418 MS.gene77192:CDS 45.0%
CAGAAATTAGAGAGCCACCT+TGG + chr2.4:31536624-31536643 MS.gene77192:CDS 45.0%
CCAATAGCTAGACATGCCTA+TGG - chr2.4:31536108-31536127 None:intergenic 45.0%
CCATAGGCATGTCTAGCTAT+TGG + chr2.4:31536105-31536124 MS.gene77192:CDS 45.0%
CCTATGGATGAACATCCTTG+AGG - chr2.4:31536092-31536111 None:intergenic 45.0%
CCTCAAGGATGTTCATCCAT+AGG + chr2.4:31536089-31536108 MS.gene77192:CDS 45.0%
GAGGAGTAAAGGTGAACATG+TGG + chr2.4:31536310-31536329 MS.gene77192:CDS 45.0%
GCTTCAACACCTCTCTTGAT+TGG - chr2.4:31535780-31535799 None:intergenic 45.0%
GTGAAAGTTCCTCCTAGCAT+TGG - chr2.4:31536215-31536234 None:intergenic 45.0%
GTGTTATGCAAGGTGTGAGA+GGG + chr2.4:31536513-31536532 MS.gene77192:CDS 45.0%
TGAATTGGGGTCCAAAGGAA+GGG + chr2.4:31536660-31536679 MS.gene77192:CDS 45.0%
TGAGATGAATTGGGGTCCAA+AGG + chr2.4:31536655-31536674 MS.gene77192:CDS 45.0%
TGCGAGTCCAATTGAACCAA+CGG + chr2.4:31536055-31536074 MS.gene77192:CDS 45.0%
TGGATGAACATCCTTGAGGA+AGG - chr2.4:31536088-31536107 None:intergenic 45.0%
TGTGTTATGCAAGGTGTGAG+AGG + chr2.4:31536512-31536531 MS.gene77192:CDS 45.0%
! ACTGGGAGCATGTGACATTA+AGG + chr2.4:31536336-31536355 MS.gene77192:CDS 45.0%
! CTGGGAGCATGTGACATTAA+GGG + chr2.4:31536337-31536356 MS.gene77192:CDS 45.0%
! GTGTGAGAGGGTTTGGTATA+AGG + chr2.4:31536525-31536544 MS.gene77192:CDS 45.0%
!! ATGGTCATGCCTTGTTTCCT+AGG + chr2.4:31536483-31536502 MS.gene77192:CDS 45.0%
AAGGTGAACATGTGGGAGAC+TGG + chr2.4:31536318-31536337 MS.gene77192:CDS 50.0%
ACACCTTGCATAACACACCC+TGG - chr2.4:31536509-31536528 None:intergenic 50.0%
ACGGGATCAAACCTTCCTCA+AGG + chr2.4:31536074-31536093 MS.gene77192:CDS 50.0%
AGGTGAACATGTGGGAGACT+GGG + chr2.4:31536319-31536338 MS.gene77192:CDS 50.0%
ATGCAAGGTGTGAGAGGGTT+TGG + chr2.4:31536518-31536537 MS.gene77192:CDS 50.0%
CCTTCCACATTGAGCAAGGA+AGG - chr2.4:31535823-31535842 None:intergenic 50.0%
CCTTCCTTGCTCAATGTGGA+AGG + chr2.4:31535820-31535839 MS.gene77192:CDS 50.0%
GCGAGTCCAATTGAACCAAC+GGG + chr2.4:31536056-31536075 MS.gene77192:CDS 50.0%
GCTCTTGCACCCCCATTAAA+TGG - chr2.4:31536263-31536282 None:intergenic 50.0%
GGAACCTTCCTTGCTCAATG+TGG + chr2.4:31535816-31535835 MS.gene77192:CDS 50.0%
TCTCAGCTTCATCCATAGGC+AGG - chr2.4:31536136-31536155 None:intergenic 50.0%
TGAGGAAGGTTTGATCCCGT+TGG - chr2.4:31536074-31536093 None:intergenic 50.0%
! CTATTGGTTGGACCTGCCTA+TGG + chr2.4:31536121-31536140 MS.gene77192:CDS 50.0%
!! ATGCCTTGTTTCCTAGGCCA+GGG + chr2.4:31536489-31536508 MS.gene77192:CDS 50.0%
ACACCCTGGCCTAGGAAACA+AGG - chr2.4:31536495-31536514 None:intergenic 55.0%
AGGCCAGGGTGTGTTATGCA+AGG + chr2.4:31536503-31536522 MS.gene77192:CDS 55.0%
GGACCTTTAGGCCCTTCCTT+TGG - chr2.4:31536674-31536693 None:intergenic 55.0%
GGTCCAAAGGAAGGGCCTAA+AGG + chr2.4:31536668-31536687 MS.gene77192:CDS 55.0%
TGCATAACACACCCTGGCCT+AGG - chr2.4:31536503-31536522 None:intergenic 55.0%
!! CATGCCTTGTTTCCTAGGCC+AGG + chr2.4:31536488-31536507 MS.gene77192:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 31535678 31536926 31535678 ID=MS.gene77192
chr2.4 mRNA 31535678 31536926 31535678 ID=MS.gene77192.t1;Parent=MS.gene77192
chr2.4 exon 31535678 31536726 31535678 ID=MS.gene77192.t1.exon1;Parent=MS.gene77192.t1
chr2.4 CDS 31535678 31536726 31535678 ID=cds.MS.gene77192.t1;Parent=MS.gene77192.t1
chr2.4 exon 31536863 31536926 31536863 ID=MS.gene77192.t1.exon2;Parent=MS.gene77192.t1
chr2.4 CDS 31536863 31536926 31536863 ID=cds.MS.gene77192.t1;Parent=MS.gene77192.t1
Gene Sequence

>MS.gene77192

ATGTGTGTTAGATCAGACCTCACTGATGAATGTATCAAATACAAGTCAATGAAGCTTTGGAGAACAGAAAACAAGAGGTTCAATGTTTATGATGTTAGACCAATCAAGAGAGGTGTTGAAGCTAAAGGTGTTTATGTAGGAACCTTCCTTGCTCAATGTGGAAGGAAAAATTCTAAAACTTTGCCTATTGTTTGTTTGAAAAATACCAATGCTAATAGATTTTCTTCCATGCCTAATTTACACCAAATTGAGACATTAATCAAGGCTTACTCTCCATACATGTATTTGCATCCTATGGATAAGTACCTTCCATCTTCTGTGGAATGGTTTTTCATAAATGGGGCAATACTCTACCAAAAAAGAGATGGTGTCGTTAATGCGAGTCCAATTGAACCAACGGGATCAAACCTTCCTCAAGGATGTTCATCCATAGGCATGTCTAGCTATTGGTTGGACCTGCCTATGGATGAAGCTGAGAAAGAAAGAGTCAAAAAAGGAGATTTACAAAGTTCCAAAGCTTATGTTCATGTAAAACCAATGCTAGGAGGAACTTTCACTGATTTAGTCATGTGGGTTTTCTATCCATTTAATGGGGGTGCAAGAGCAAAAGTAGCATTCATGAACATTCCATTGAGGAGTAAAGGTGAACATGTGGGAGACTGGGAGCATGTGACATTAAGGGTAAGCAACTTTAGTGGAGAATTATGGAAGGTATATTTGTCACAACATAGCAAGGGTCAATGGGTTGATGCATGTGACCTTGAGTTTAAAAATGGAAATAGACCTGTGGTTTATTCTTCATTGCATGGTCATGCCTTGTTTCCTAGGCCAGGGTGTGTTATGCAAGGTGTGAGAGGGTTTGGTATAAGGAATGATGCATGTAAGAGTGATTTGGTGATGGATATGGTAAAAGGGTATGAAATAGTTGCTGATGAATATTTGGGGTCAGAAATTAGAGAGCCACCTTGGCTTAACTATGAGATGAATTGGGGTCCAAAGGAAGGGCCTAAAGGTCCAAAACAAAAGGATTTTTGGAAAGGAGATGAACGGTGAGTGTATTGTCAATTTGACATTCTCAATATTCGTCTTAATTGGTTTTGTATTTTTTCCATAATGTTTTTGTATACCCTATTGTTTATACTTATGGTATTTATTTTCTCTTTTTTCTTTTGGTATAAATTTTAGGTGGCATTTAACATGGACCAATGTAAAACTTGCCCATGATGGACCACTTGTGATTATTGGCTAG
Protein sequence

>MS.gene77192.t1

MCVRSDLTDECIKYKSMKLWRTENKRFNVYDVRPIKRGVEAKGVYVGTFLAQCGRKNSKTLPIVCLKNTNANRFSSMPNLHQIETLIKAYSPYMYLHPMDKYLPSSVEWFFINGAILYQKRDGVVNASPIEPTGSNLPQGCSSIGMSSYWLDLPMDEAEKERVKKGDLQSSKAYVHVKPMLGGTFTDLVMWVFYPFNGGARAKVAFMNIPLRSKGEHVGDWEHVTLRVSNFSGELWKVYLSQHSKGQWVDACDLEFKNGNRPVVYSSLHGHALFPRPGCVMQGVRGFGIRNDACKSDLVMDMVKGYEIVADEYLGSEIREPPWLNYEMNWGPKEGPKGPKQKDFWKGDERWHLTWTNVKLAHDGPLVIIG