AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77630.t1 MTR_0036s0070 88.462 338 36 1 1 338 93 427 0.0 620
MS.gene77630.t1 MTR_0036s0050 75.802 343 77 1 1 337 93 435 0.0 533
MS.gene77630.t1 MTR_0036s0110 60.519 347 126 3 1 337 97 442 4.33e-145 417
MS.gene77630.t1 MTR_1g060750 55.263 342 129 5 1 337 54 376 6.34e-122 355
MS.gene77630.t1 MTR_8g096990 46.132 349 170 8 1 337 89 431 5.46e-101 304
MS.gene77630.t1 MTR_5g080890 43.305 351 179 8 1 337 89 433 4.61e-93 284
MS.gene77630.t1 MTR_5g080960 43.020 351 180 8 1 337 89 433 9.61e-93 283
MS.gene77630.t1 MTR_5g044770 42.651 347 184 7 1 337 89 430 3.12e-92 282
MS.gene77630.t1 MTR_8g038280 40.909 352 188 8 1 337 89 435 1.94e-88 272
MS.gene77630.t1 MTR_8g038250 42.373 354 160 9 1 337 89 415 4.23e-85 263
MS.gene77630.t1 MTR_6g090290 38.810 353 195 7 1 337 89 436 9.63e-81 253
MS.gene77630.t1 MTR_2g059490 41.926 353 184 9 1 336 61 409 7.33e-79 248
MS.gene77630.t1 MTR_8g038310 40.173 346 176 8 1 337 90 413 5.17e-76 239
MS.gene77630.t1 MTR_2g059460 39.266 354 189 10 1 337 62 406 6.04e-76 239
MS.gene77630.t1 MTR_5g008110 40.741 351 189 10 1 337 70 415 3.35e-75 238
MS.gene77630.t1 MTR_3g097150 40.625 352 186 9 1 337 105 448 4.80e-75 238
MS.gene77630.t1 MTR_4g109760 41.471 340 178 9 11 337 67 398 6.40e-73 231
MS.gene77630.t1 MTR_3g028120 38.571 350 199 7 1 337 92 438 1.25e-72 231
MS.gene77630.t1 MTR_2g067830 38.261 345 199 7 5 337 64 406 2.33e-72 230
MS.gene77630.t1 MTR_5g008150 39.660 353 191 11 1 337 66 412 9.54e-72 228
MS.gene77630.t1 MTR_2g105300 37.879 330 191 7 17 334 54 381 1.07e-70 225
MS.gene77630.t1 MTR_0024s0040 38.527 353 193 8 1 337 66 410 2.10e-70 225
MS.gene77630.t1 MTR_5g008070 40.805 348 188 11 1 336 84 425 1.25e-69 223
MS.gene77630.t1 MTR_5g038360 36.261 353 204 9 1 338 91 437 1.75e-67 218
MS.gene77630.t1 MTR_6g088760 38.080 323 183 7 5 313 95 414 3.73e-65 214
MS.gene77630.t1 MTR_5g044570 36.571 350 179 10 1 337 92 411 3.86e-62 204
MS.gene77630.t1 MTR_4g045857 49.289 211 94 6 1 203 61 266 1.53e-53 178
MS.gene77630.t1 MTR_7g117430 35.613 351 198 13 2 335 145 484 5.63e-52 179
MS.gene77630.t1 MTR_5g008090 35.786 299 174 8 53 337 20 314 1.12e-47 163
MS.gene77630.t1 MTR_3g046300 35.054 368 203 13 1 338 100 461 3.18e-46 163
MS.gene77630.t1 MTR_5g091130 34.247 365 207 13 1 336 194 554 1.55e-45 163
MS.gene77630.t1 MTR_1g115210 32.955 352 205 13 1 335 149 486 3.42e-44 158
MS.gene77630.t1 MTR_1g039270 31.534 352 211 14 1 335 131 469 1.10e-43 156
MS.gene77630.t1 MTR_1g111350 32.764 351 201 12 2 335 135 467 1.76e-42 153
MS.gene77630.t1 MTR_4g007740 34.320 338 196 11 17 336 149 478 1.85e-41 150
MS.gene77630.t1 MTR_6g085010 31.014 345 214 10 2 335 160 491 4.43e-41 150
MS.gene77630.t1 MTR_2g028190 31.054 351 222 11 1 335 105 451 5.78e-37 138
MS.gene77630.t1 MTR_3g053710 31.180 356 207 13 5 335 124 466 2.45e-33 128
MS.gene77630.t1 MTR_2g055840 27.147 361 230 9 3 335 18 373 5.03e-32 123
MS.gene77630.t1 MTR_3g053980 31.988 347 210 12 5 335 149 485 4.44e-31 122
MS.gene77630.t1 MTR_2g084550 31.903 373 205 20 2 338 81 440 3.19e-30 120
MS.gene77630.t1 MTR_4g056420 30.055 366 216 20 2 336 81 437 3.51e-30 120
MS.gene77630.t1 MTR_5g016260 31.680 363 200 20 2 337 92 433 8.61e-29 117
MS.gene77630.t1 MTR_7g105850 30.867 392 201 20 1 338 93 468 9.97e-29 115
MS.gene77630.t1 MTR_5g016260 31.680 363 200 20 2 337 92 433 1.05e-28 117
MS.gene77630.t1 MTR_3g065160 28.032 371 206 14 5 337 81 428 2.93e-27 111
MS.gene77630.t1 MTR_7g080230 28.415 366 226 12 2 338 75 433 3.51e-27 111
MS.gene77630.t1 MTR_8g103880 27.003 337 220 10 1 323 119 443 3.54e-26 108
MS.gene77630.t1 MTR_7g021510 27.763 371 224 17 2 338 76 436 1.18e-25 107
MS.gene77630.t1 MTR_2g070430 31.053 380 207 22 1 337 90 457 1.65e-25 106
MS.gene77630.t1 MTR_3g068000 27.907 387 206 16 1 335 76 441 5.21e-25 105
MS.gene77630.t1 MTR_7g102750 29.111 371 213 18 2 336 85 441 1.13e-24 104
MS.gene77630.t1 MTR_1g061940 30.295 373 212 20 2 338 87 447 1.37e-24 104
MS.gene77630.t1 MTR_4g100990 28.093 388 202 16 17 336 93 471 2.04e-24 103
MS.gene77630.t1 MTR_1g078240 29.189 370 219 17 2 338 73 432 2.65e-24 103
MS.gene77630.t1 MTR_7g102750 31.056 322 177 17 2 292 85 392 1.14e-23 101
MS.gene77630.t1 MTR_1g078240 28.571 371 221 16 2 338 73 433 1.68e-23 101
MS.gene77630.t1 MTR_4g095270 28.255 361 215 17 2 337 84 425 1.89e-23 101
MS.gene77630.t1 MTR_3g014290 30.199 351 214 12 1 336 83 417 2.05e-23 100
MS.gene77630.t1 MTR_1g037630 28.205 351 221 12 1 336 95 429 2.30e-23 100
MS.gene77630.t1 MTR_4g095270 28.255 361 215 17 2 337 84 425 2.34e-23 101
MS.gene77630.t1 MTR_5g061690 28.529 340 212 13 3 323 106 433 8.03e-22 96.7
MS.gene77630.t1 MTR_1g078240 32.824 262 144 11 2 240 73 325 3.28e-21 93.6
MS.gene77630.t1 MTR_8g039540 28.490 351 221 12 1 336 99 434 2.27e-20 91.7
MS.gene77630.t1 MTR_5g012490 24.874 398 218 16 8 336 85 470 2.84e-20 91.7
MS.gene77630.t1 MTR_8g075010 25.620 363 222 14 3 335 105 449 5.85e-20 90.9
MS.gene77630.t1 MTR_5g077850 27.968 379 200 15 5 337 88 439 1.17e-19 89.7
MS.gene77630.t1 MTR_7g062360 26.075 372 230 14 1 335 62 425 2.76e-19 88.6
MS.gene77630.t1 MTR_1g029810 25.479 365 231 13 1 337 60 411 5.50e-19 87.4
MS.gene77630.t1 MTR_5g022880 26.050 357 228 14 3 335 117 461 5.96e-19 88.2
MS.gene77630.t1 MTR_1g029810 25.479 365 231 13 1 337 60 411 6.25e-19 87.4
MS.gene77630.t1 MTR_7g080230 27.114 343 217 12 22 338 28 363 2.56e-18 85.5
MS.gene77630.t1 MTR_6g084710 28.295 258 169 10 3 251 196 446 3.87e-18 85.9
MS.gene77630.t1 MTR_6g084710 28.295 258 169 10 3 251 196 446 4.00e-18 85.5
MS.gene77630.t1 MTR_8g094590 24.860 358 231 14 5 336 80 425 6.28e-18 84.7
MS.gene77630.t1 MTR_3g087780 26.316 361 224 15 3 335 104 450 7.74e-18 84.7
MS.gene77630.t1 MTR_4g008110 39.823 113 42 3 75 184 48 137 2.15e-17 78.2
MS.gene77630.t1 MTR_8g094590 29.339 242 153 9 5 240 80 309 1.02e-16 80.1
MS.gene77630.t1 MTR_8g094590 27.692 260 170 9 5 258 80 327 1.30e-16 80.5
MS.gene77630.t1 MTR_8g075090 25.070 359 231 14 3 337 117 461 3.73e-15 76.6
MS.gene77630.t1 MTR_8g075090 25.210 357 229 14 3 335 117 459 7.13e-15 75.5
MS.gene77630.t1 MTR_0945s0010 31.707 123 81 2 217 336 1 123 2.38e-14 70.5
MS.gene77630.t1 MTR_4g023630 24.390 369 236 14 1 337 64 421 6.74e-14 72.4
MS.gene77630.t1 MTR_3g100500 28.652 356 215 13 1 335 100 437 1.27e-13 71.6
MS.gene77630.t1 MTR_8g075090 26.347 334 208 14 3 312 117 436 3.64e-13 70.5
MS.gene77630.t1 MTR_5g077920 25.263 380 213 16 5 338 75 429 3.50e-11 64.3
MS.gene77630.t1 MTR_2g037710 26.923 208 125 9 140 336 15 206 7.89e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77630.t1 AT1G31450 45.404 359 170 9 1 336 87 442 7.65e-91 279
MS.gene77630.t1 AT5G33340 45.533 347 177 4 1 337 92 436 9.25e-91 278
MS.gene77630.t1 AT1G64830 44.669 347 178 6 1 337 88 430 7.33e-88 271
MS.gene77630.t1 AT2G35615 44.321 361 173 9 1 336 87 444 1.41e-87 270
MS.gene77630.t1 AT2G28010 39.886 351 173 11 1 337 67 393 3.60e-72 229
MS.gene77630.t1 AT2G28030 39.031 351 176 11 1 337 63 389 2.66e-69 221
MS.gene77630.t1 AT2G03200 39.444 360 187 10 1 337 109 460 1.25e-68 222
MS.gene77630.t1 AT2G28040 38.793 348 178 10 1 336 67 391 1.82e-66 214
MS.gene77630.t1 AT2G28040 38.793 348 178 10 1 336 58 382 2.20e-66 214
MS.gene77630.t1 AT2G28220 39.377 353 176 14 1 337 69 399 2.73e-64 209
MS.gene77630.t1 AT2G28225 38.636 352 180 10 1 337 69 399 7.79e-64 208
MS.gene77630.t1 AT1G01300 33.616 354 203 13 2 336 145 485 3.30e-47 166
MS.gene77630.t1 AT3G18490 31.609 348 212 12 2 335 165 500 5.94e-46 163
MS.gene77630.t1 AT3G61820 34.734 357 201 12 1 335 137 483 3.06e-45 161
MS.gene77630.t1 AT2G23945 32.703 370 191 17 5 337 102 450 9.27e-44 156
MS.gene77630.t1 AT2G42980 32.546 381 195 12 1 336 162 525 9.66e-43 155
MS.gene77630.t1 AT4G30030 33.989 356 194 17 3 335 82 419 3.35e-42 152
MS.gene77630.t1 AT3G59080 32.345 371 207 15 1 336 172 533 7.75e-41 150
MS.gene77630.t1 AT3G25700 29.380 371 221 12 1 336 86 450 1.34e-40 148
MS.gene77630.t1 AT3G20015 31.714 350 212 11 1 335 133 470 4.85e-40 147
MS.gene77630.t1 AT1G25510 33.333 348 203 14 2 335 151 483 9.85e-40 146
MS.gene77630.t1 AT4G30040 31.667 360 197 14 1 330 87 427 1.84e-39 144
MS.gene77630.t1 AT5G10770 33.048 351 210 11 1 336 134 474 8.27e-39 144
MS.gene77630.t1 AT1G79720 32.565 347 193 11 16 335 150 482 1.33e-35 135
MS.gene77630.t1 AT5G36260 29.781 366 218 13 2 338 81 436 3.57e-34 131
MS.gene77630.t1 AT5G10760 34.393 346 201 13 1 335 134 464 4.31e-33 128
MS.gene77630.t1 AT3G12700 28.291 357 230 11 2 336 109 461 3.65e-31 122
MS.gene77630.t1 AT3G02740 29.121 364 218 15 2 336 13 365 1.10e-29 117
MS.gene77630.t1 AT3G02740 29.121 364 218 15 2 336 88 440 2.61e-29 117
MS.gene77630.t1 AT3G59080 30.978 368 180 15 1 336 172 497 7.13e-29 116
MS.gene77630.t1 AT1G66180 28.267 375 211 16 1 337 74 428 2.66e-28 114
MS.gene77630.t1 AT3G54400 30.532 357 206 16 1 336 90 425 4.32e-27 110
MS.gene77630.t1 AT4G12920 28.653 349 212 14 2 335 61 387 4.50e-27 110
MS.gene77630.t1 AT3G50050 29.558 362 210 16 2 337 96 438 3.41e-26 109
MS.gene77630.t1 AT5G37540 28.226 372 215 14 1 335 82 438 1.13e-25 107
MS.gene77630.t1 AT5G43100 27.933 358 217 15 2 336 79 418 2.25e-25 106
MS.gene77630.t1 AT1G05840 28.108 370 222 15 2 338 83 441 2.48e-25 106
MS.gene77630.t1 AT5G22850 30.000 370 216 20 2 338 84 443 4.14e-25 105
MS.gene77630.t1 AT5G43100 27.933 358 217 15 2 336 79 418 6.23e-25 105
MS.gene77630.t1 AT1G08210 30.411 365 214 16 2 336 87 441 7.77e-25 105
MS.gene77630.t1 AT5G45120 27.792 403 214 17 1 335 85 478 8.67e-25 105
MS.gene77630.t1 AT1G08210 30.411 365 214 16 2 336 121 475 1.06e-24 105
MS.gene77630.t1 AT1G08210 30.411 365 214 16 2 336 135 489 1.06e-24 105
MS.gene77630.t1 AT2G36670 29.076 368 220 17 2 338 37 394 1.61e-24 103
MS.gene77630.t1 AT2G36670 29.076 368 220 17 2 338 37 394 1.61e-24 103
MS.gene77630.t1 AT2G36670 29.076 368 220 17 2 338 103 460 1.91e-24 103
MS.gene77630.t1 AT2G36670 29.076 368 220 17 2 338 108 465 2.28e-24 103
MS.gene77630.t1 AT2G39710 28.075 374 212 16 1 331 67 426 1.18e-23 101
MS.gene77630.t1 AT1G08210 29.337 392 210 17 2 336 87 468 1.66e-23 101
MS.gene77630.t1 AT1G09750 26.761 355 228 13 1 335 106 448 2.20e-23 100
MS.gene77630.t1 AT1G65240 26.503 366 227 15 2 338 77 429 2.94e-23 100
MS.gene77630.t1 AT5G02190 26.954 371 218 15 8 335 82 442 6.41e-22 96.3
MS.gene77630.t1 AT5G24820 26.431 367 223 12 1 335 48 399 2.48e-21 94.4
MS.gene77630.t1 AT3G52500 28.889 405 191 20 1 336 92 468 5.11e-21 94.0
MS.gene77630.t1 AT2G17760 24.650 357 230 15 3 335 108 449 2.36e-19 89.4
MS.gene77630.t1 AT4G16563 27.431 401 201 16 17 336 101 492 3.46e-19 88.6
MS.gene77630.t1 AT1G49050 26.772 254 163 12 2 240 206 451 2.40e-17 83.2
MS.gene77630.t1 AT5G07030 26.287 369 209 16 1 336 117 455 5.04e-16 79.0
MS.gene77630.t1 AT3G51360 25.556 360 230 16 3 338 9 354 1.70e-15 77.4
MS.gene77630.t1 AT3G51360 25.556 360 230 16 3 338 93 438 2.28e-15 77.4
MS.gene77630.t1 AT3G51360 25.556 360 230 16 3 338 103 448 2.28e-15 77.4
MS.gene77630.t1 AT3G42550 25.620 363 187 13 3 336 82 390 3.60e-15 76.6
MS.gene77630.t1 AT1G44130 23.784 370 226 15 3 337 53 401 6.10e-14 72.8
MS.gene77630.t1 AT3G51340 22.191 356 239 12 3 335 107 447 4.35e-13 70.5
MS.gene77630.t1 AT3G25700 35.200 125 70 5 1 115 86 209 5.06e-13 69.7
MS.gene77630.t1 AT3G51340 23.179 302 196 11 3 283 107 393 4.82e-11 63.9

Find 70 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACACGGGGAGTGACCTTATT+TGG 0.099816 2.3:+29907339 MS.gene77630:CDS
GAACTAAGTTCTGAGGTTTA+AGG 0.180786 2.3:-29908132 None:intergenic
ACCAATGATCTTTCCATCTT+TGG 0.184135 2.3:+29908196 MS.gene77630:CDS
TTGCTATTTGAGTTTAAATT+TGG 0.187752 2.3:-29907761 None:intergenic
CTCTTACACTATTGGAGATT+TGG 0.195926 2.3:+29907541 MS.gene77630:CDS
GTCAAATGAAACACAAAATT+AGG 0.203457 2.3:-29908094 None:intergenic
TCAAATAGCAAAATGAAATT+TGG 0.248015 2.3:+29907773 MS.gene77630:CDS
CCACATCCATGGTAGCCTTT+GGG 0.253599 2.3:-29907476 None:intergenic
GGAGATGAAGCAATTATAAA+AGG 0.259280 2.3:+29907794 MS.gene77630:CDS
AACAAAATCTTCCACTTTCA+TGG 0.282987 2.3:+29907418 MS.gene77630:CDS
CAACTAGACCCGTAGCTTTA+CGG 0.294559 2.3:-29907658 None:intergenic
GGTGATAACTCTTACACTAT+TGG 0.311974 2.3:+29907533 MS.gene77630:CDS
TCTTACACTATTGGAGATTT+GGG 0.319645 2.3:+29907542 MS.gene77630:CDS
TCCACATCCATGGTAGCCTT+TGG 0.338920 2.3:-29907477 None:intergenic
AACTAAGTTCTGAGGTTTAA+GGG 0.357303 2.3:-29908131 None:intergenic
CAGAACTTAGTTCTTGATCT+TGG 0.360634 2.3:+29908142 MS.gene77630:CDS
GCTTCCGTGAAGGGAGTTAT+TGG 0.362584 2.3:+29908001 MS.gene77630:CDS
CGACAACTCGCATTGCTTGT+TGG 0.369719 2.3:+29908165 MS.gene77630:CDS
ATAATTGGAAACTTAGGTCT+TGG 0.376014 2.3:-29908296 None:intergenic
GGTAACATAATCATTGATTC+AGG 0.379513 2.3:+29907932 MS.gene77630:CDS
TCCAAAGATGGAAAGATCAT+TGG 0.384929 2.3:-29908197 None:intergenic
GGGAACATTGTACCCAAATA+AGG 0.386073 2.3:-29907352 None:intergenic
CACGGGGAGTGACCTTATTT+GGG 0.389068 2.3:+29907340 MS.gene77630:CDS
TCAAATGAAACACAAAATTA+GGG 0.392395 2.3:-29908093 None:intergenic
ATTTGGAGGAATCAAACAAT+AGG 0.396296 2.3:-29907744 None:intergenic
GGTACTGAATCCATTAACTT+TGG 0.398522 2.3:+29907563 MS.gene77630:CDS
TTGTGCTCTATTTCCAAAGA+TGG 0.401939 2.3:-29908209 None:intergenic
TGTGAGTATATATACACTTA+TGG 0.402832 2.3:+29907512 MS.gene77630:CDS
CTATTTGAGTTTAAATTTGG+AGG 0.456554 2.3:-29907758 None:intergenic
CACATCCATGGTAGCCTTTG+GGG 0.466661 2.3:-29907475 None:intergenic
TACCTCATGAAAATCTATAT+AGG 0.467318 2.3:+29907284 None:intergenic
ACAAAATCTTCCACTTTCAT+GGG 0.473834 2.3:+29907419 MS.gene77630:CDS
GCTACGGGTCTAGTTGGTCT+TGG 0.478890 2.3:+29907665 MS.gene77630:CDS
GCAGCAGCAAACCTTTCAAC+AGG 0.481402 2.3:-29907314 None:intergenic
CGGTGATGCCTTCGAGATTG+AGG 0.489988 2.3:-29907874 None:intergenic
AACACCAATAACTCCCTTCA+CGG 0.491057 2.3:-29908005 None:intergenic
TCACAACTAGGTGATTCAAT+TGG 0.503436 2.3:+29907710 MS.gene77630:CDS
ACATGACAAACCCATGAAAG+TGG 0.505680 2.3:-29907429 None:intergenic
TAAAAGAAGCCGTAAAGCTA+CGG 0.507090 2.3:+29907649 MS.gene77630:CDS
CGTAAAGCTACGGGTCTAGT+TGG 0.511569 2.3:+29907659 MS.gene77630:CDS
GGTCTAGTTGGTCTTGGAGC+AGG 0.513423 2.3:+29907671 MS.gene77630:CDS
GAAGCAATTATAAAAGGTAA+TGG 0.516167 2.3:+29907800 MS.gene77630:CDS
AGGTTTGCTGCTGCAGACAC+GGG 0.529583 2.3:+29907323 MS.gene77630:CDS
CCCAAAGGCTACCATGGATG+TGG 0.533147 2.3:+29907476 MS.gene77630:CDS
CTATCACTAGTTTCACAACT+AGG 0.533914 2.3:+29907698 MS.gene77630:CDS
AAGGTTTGCTGCTGCAGACA+CGG 0.537738 2.3:+29907322 MS.gene77630:CDS
AAAAGAAGCCGTAAAGCTAC+GGG 0.541737 2.3:+29907650 MS.gene77630:CDS
TAGGTCTTGGAACAATCAGT+TGG 0.542995 2.3:-29908283 None:intergenic
GGAACAATCAGTTGGAGCAA+AGG 0.550575 2.3:-29908275 None:intergenic
TTACTCCCCAAAGGCTACCA+TGG 0.551474 2.3:+29907470 MS.gene77630:CDS
AGTTGTGAAACTAGTGATAG+TGG 0.555072 2.3:-29907695 None:intergenic
GAAGGGAGTTATTGGTGTTG+AGG 0.557503 2.3:+29908009 MS.gene77630:CDS
GATTTGGTAGCTTCCGTGAA+GGG 0.562048 2.3:+29907992 MS.gene77630:CDS
TCATAATAAGGAGTGTCTTG+TGG 0.564393 2.3:-29907392 None:intergenic
GAACTTTGTTATATCTCCAA+AGG 0.567428 2.3:-29908071 None:intergenic
TAGGCACCCCACCTGTTGAA+AGG 0.569203 2.3:+29907303 MS.gene77630:CDS
CAGCAAACCTTTCAACAGGT+GGG 0.572331 2.3:-29907310 None:intergenic
GCTCAGTCAGGTCGAACTGA+TGG 0.572601 2.3:+29907911 MS.gene77630:CDS
AATCTCGAAGGCATCACCGT+TGG 0.579467 2.3:+29907878 MS.gene77630:CDS
TTAGTAGGGTACGATCTCAA+AGG 0.581764 2.3:+29908241 MS.gene77630:CDS
GCAGCAAACCTTTCAACAGG+TGG 0.583569 2.3:-29907311 None:intergenic
AGATCAAGAACTAAGTTCTG+AGG 0.590877 2.3:-29908139 None:intergenic
ATTCGAAGATTTGATTACGA+GGG 0.592789 2.3:-29907837 None:intergenic
AGCAAACCTTTCAACAGGTG+GGG 0.603962 2.3:-29907309 None:intergenic
TGATTTGGTAGCTTCCGTGA+AGG 0.607826 2.3:+29907991 MS.gene77630:CDS
TTCGAAGATTTGATTACGAG+GGG 0.611570 2.3:-29907836 None:intergenic
AAGATGGAAAGATCATTGGT+TGG 0.625377 2.3:-29908193 None:intergenic
TCTTGCTCATTACTCCCCAA+AGG 0.655591 2.3:+29907461 MS.gene77630:CDS
GGTTTGCTGCTGCAGACACG+GGG 0.669259 2.3:+29907324 MS.gene77630:CDS
GATTCGAAGATTTGATTACG+AGG 0.682733 2.3:-29907838 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACAATTTAATTTTTTAGTA+GGG + chr2.3:29908227-29908246 MS.gene77630:CDS 15.0%
!! TCAAATAGCAAAATGAAATT+TGG + chr2.3:29907773-29907792 MS.gene77630:CDS 20.0%
!! TCAAATGAAACACAAAATTA+GGG - chr2.3:29908096-29908115 None:intergenic 20.0%
!!! ACAAAACTTTTACAATGATT+TGG + chr2.3:29907976-29907995 MS.gene77630:CDS 20.0%
!!! ACATTTCCTAAATCAATTTT+TGG + chr2.3:29907599-29907618 MS.gene77630:CDS 20.0%
!!! GCACAATTTAATTTTTTAGT+AGG + chr2.3:29908226-29908245 MS.gene77630:CDS 20.0%
!!! TAATTTTGTGTTTCATTTGA+CGG + chr2.3:29908096-29908115 MS.gene77630:CDS 20.0%
!!! TTGCTATTTGAGTTTAAATT+TGG - chr2.3:29907764-29907783 None:intergenic 20.0%
! GAAGCAATTATAAAAGGTAA+TGG + chr2.3:29907800-29907819 MS.gene77630:CDS 25.0%
! GTCAAATGAAACACAAAATT+AGG - chr2.3:29908097-29908116 None:intergenic 25.0%
! TGTGAGTATATATACACTTA+TGG + chr2.3:29907512-29907531 MS.gene77630:CDS 25.0%
!! AATTTTGTGTTTCATTTGAC+GGG + chr2.3:29908097-29908116 MS.gene77630:CDS 25.0%
!! ATCTTTTTCTCCAAAGTTAA+TGG - chr2.3:29907576-29907595 None:intergenic 25.0%
!! CTATTTGAGTTTAAATTTGG+AGG - chr2.3:29907761-29907780 None:intergenic 25.0%
!! GATTTTGTTGGATCATAATA+AGG - chr2.3:29907407-29907426 None:intergenic 25.0%
AACAAAATCTTCCACTTTCA+TGG + chr2.3:29907418-29907437 MS.gene77630:CDS 30.0%
ACAAAATCTTCCACTTTCAT+GGG + chr2.3:29907419-29907438 MS.gene77630:CDS 30.0%
AGATTGAGGAAGTAAAAAGA+AGG - chr2.3:29907863-29907882 None:intergenic 30.0%
ATTCGAAGATTTGATTACGA+GGG - chr2.3:29907840-29907859 None:intergenic 30.0%
ATTTGGAGGAATCAAACAAT+AGG - chr2.3:29907747-29907766 None:intergenic 30.0%
GGAGATGAAGCAATTATAAA+AGG + chr2.3:29907794-29907813 MS.gene77630:CDS 30.0%
TCTTACACTATTGGAGATTT+GGG + chr2.3:29907542-29907561 MS.gene77630:CDS 30.0%
! AAGCAAAAAGTATATGGTGA+TGG - chr2.3:29908049-29908068 None:intergenic 30.0%
! AAGGTGAAGCAAAAAGTATA+TGG - chr2.3:29908055-29908074 None:intergenic 30.0%
! ATGAAAGTGGAAGATTTTGT+TGG - chr2.3:29907419-29907438 None:intergenic 30.0%
! CCACATCCAAAAATTGATTT+AGG - chr2.3:29907608-29907627 None:intergenic 30.0%
! GAACTTTGTTATATCTCCAA+AGG - chr2.3:29908074-29908093 None:intergenic 30.0%
! GTGGAAAACAATTTTCACAA+GGG - chr2.3:29907376-29907395 None:intergenic 30.0%
! TGGAAAACAATTTTCACAAG+GGG - chr2.3:29907375-29907394 None:intergenic 30.0%
! TGTGGAAAACAATTTTCACA+AGG - chr2.3:29907377-29907396 None:intergenic 30.0%
!! AACTAAGTTCTGAGGTTTAA+GGG - chr2.3:29908134-29908153 None:intergenic 30.0%
!! CCTAAATCAATTTTTGGATG+TGG + chr2.3:29907605-29907624 MS.gene77630:CDS 30.0%
!! GGTAACATAATCATTGATTC+AGG + chr2.3:29907932-29907951 MS.gene77630:CDS 30.0%
!! TATACTTTTTGCTTCACCTT+TGG + chr2.3:29908055-29908074 MS.gene77630:CDS 30.0%
AAGATGGAAAGATCATTGGT+TGG - chr2.3:29908196-29908215 None:intergenic 35.0%
ACCAATGATCTTTCCATCTT+TGG + chr2.3:29908196-29908215 MS.gene77630:CDS 35.0%
AGATCAAGAACTAAGTTCTG+AGG - chr2.3:29908142-29908161 None:intergenic 35.0%
AGTTGTGAAACTAGTGATAG+TGG - chr2.3:29907698-29907717 None:intergenic 35.0%
CTCTTACACTATTGGAGATT+TGG + chr2.3:29907541-29907560 MS.gene77630:CDS 35.0%
GATTCGAAGATTTGATTACG+AGG - chr2.3:29907841-29907860 None:intergenic 35.0%
GGTGATAACTCTTACACTAT+TGG + chr2.3:29907533-29907552 MS.gene77630:CDS 35.0%
TAAAAGAAGCCGTAAAGCTA+CGG + chr2.3:29907649-29907668 MS.gene77630:CDS 35.0%
TCACAACTAGGTGATTCAAT+TGG + chr2.3:29907710-29907729 MS.gene77630:CDS 35.0%
TCCAAAGATGGAAAGATCAT+TGG - chr2.3:29908200-29908219 None:intergenic 35.0%
TTCGAAGATTTGATTACGAG+GGG - chr2.3:29907839-29907858 None:intergenic 35.0%
TTGTGCTCTATTTCCAAAGA+TGG - chr2.3:29908212-29908231 None:intergenic 35.0%
! CAGAACTTAGTTCTTGATCT+TGG + chr2.3:29908142-29908161 MS.gene77630:CDS 35.0%
! CTATCACTAGTTTCACAACT+AGG + chr2.3:29907698-29907717 MS.gene77630:CDS 35.0%
! GGTACTGAATCCATTAACTT+TGG + chr2.3:29907563-29907582 MS.gene77630:CDS 35.0%
! TTTTACTTCCTCAATCTCGA+AGG + chr2.3:29907866-29907885 MS.gene77630:CDS 35.0%
!! CAAAAAGTATATGGTGATGG+AGG - chr2.3:29908046-29908065 None:intergenic 35.0%
!! GAACTAAGTTCTGAGGTTTA+AGG - chr2.3:29908135-29908154 None:intergenic 35.0%
!! GATTTGATTTTCCACATCCA+TGG - chr2.3:29907490-29907509 None:intergenic 35.0%
!! TCATAATAAGGAGTGTCTTG+TGG - chr2.3:29907395-29907414 None:intergenic 35.0%
AAAAGAAGCCGTAAAGCTAC+GGG + chr2.3:29907650-29907669 MS.gene77630:CDS 40.0%
AACACCAATAACTCCCTTCA+CGG - chr2.3:29908008-29908027 None:intergenic 40.0%
ACATGACAAACCCATGAAAG+TGG - chr2.3:29907432-29907451 None:intergenic 40.0%
CATCACCGTTGGACAAAAAA+GGG + chr2.3:29907889-29907908 MS.gene77630:CDS 40.0%
GGGAACATTGTACCCAAATA+AGG - chr2.3:29907355-29907374 None:intergenic 40.0%
TAGGTCTTGGAACAATCAGT+TGG - chr2.3:29908286-29908305 None:intergenic 40.0%
TTAGTAGGGTACGATCTCAA+AGG + chr2.3:29908241-29908260 MS.gene77630:CDS 40.0%
AGCAAACCTTTCAACAGGTG+GGG - chr2.3:29907312-29907331 None:intergenic 45.0%
CAGCAAACCTTTCAACAGGT+GGG - chr2.3:29907313-29907332 None:intergenic 45.0%
GCATCACCGTTGGACAAAAA+AGG + chr2.3:29907888-29907907 MS.gene77630:CDS 45.0%
GGAACAATCAGTTGGAGCAA+AGG - chr2.3:29908278-29908297 None:intergenic 45.0%
TCTTGCTCATTACTCCCCAA+AGG + chr2.3:29907461-29907480 MS.gene77630:CDS 45.0%
! CAACTAGACCCGTAGCTTTA+CGG - chr2.3:29907661-29907680 None:intergenic 45.0%
! GATTTGGTAGCTTCCGTGAA+GGG + chr2.3:29907992-29908011 MS.gene77630:CDS 45.0%
!! GAAGGGAGTTATTGGTGTTG+AGG + chr2.3:29908009-29908028 MS.gene77630:CDS 45.0%
!! TGATTTGGTAGCTTCCGTGA+AGG + chr2.3:29907991-29908010 MS.gene77630:CDS 45.0%
!!! CTGAGCCCTTTTTTGTCCAA+CGG - chr2.3:29907897-29907916 None:intergenic 45.0%
ACACGGGGAGTGACCTTATT+TGG + chr2.3:29907339-29907358 MS.gene77630:CDS 50.0%
CACATCCATGGTAGCCTTTG+GGG - chr2.3:29907478-29907497 None:intergenic 50.0%
CCACATCCATGGTAGCCTTT+GGG - chr2.3:29907479-29907498 None:intergenic 50.0%
CGACAACTCGCATTGCTTGT+TGG + chr2.3:29908165-29908184 MS.gene77630:CDS 50.0%
CGTAAAGCTACGGGTCTAGT+TGG + chr2.3:29907659-29907678 MS.gene77630:CDS 50.0%
GCAGCAAACCTTTCAACAGG+TGG - chr2.3:29907314-29907333 None:intergenic 50.0%
GCAGCAGCAAACCTTTCAAC+AGG - chr2.3:29907317-29907336 None:intergenic 50.0%
GCTTCCGTGAAGGGAGTTAT+TGG + chr2.3:29908001-29908020 MS.gene77630:CDS 50.0%
GGACAAAAAAGGGCTCAGTC+AGG + chr2.3:29907899-29907918 MS.gene77630:CDS 50.0%
TCCACATCCATGGTAGCCTT+TGG - chr2.3:29907480-29907499 None:intergenic 50.0%
TTACTCCCCAAAGGCTACCA+TGG + chr2.3:29907470-29907489 MS.gene77630:CDS 50.0%
! CACGGGGAGTGACCTTATTT+GGG + chr2.3:29907340-29907359 MS.gene77630:CDS 50.0%
!! AAGGTTTGCTGCTGCAGACA+CGG + chr2.3:29907322-29907341 MS.gene77630:CDS 50.0%
!! AATCTCGAAGGCATCACCGT+TGG + chr2.3:29907878-29907897 MS.gene77630:CDS 50.0%
CCCAAAGGCTACCATGGATG+TGG + chr2.3:29907476-29907495 MS.gene77630:CDS 55.0%
CGGTGATGCCTTCGAGATTG+AGG - chr2.3:29907877-29907896 None:intergenic 55.0%
GCTCAGTCAGGTCGAACTGA+TGG + chr2.3:29907911-29907930 MS.gene77630:CDS 55.0%
TAGGCACCCCACCTGTTGAA+AGG + chr2.3:29907303-29907322 MS.gene77630:CDS 55.0%
! GCTACGGGTCTAGTTGGTCT+TGG + chr2.3:29907665-29907684 MS.gene77630:CDS 55.0%
!! AGGTTTGCTGCTGCAGACAC+GGG + chr2.3:29907323-29907342 MS.gene77630:CDS 55.0%
!! GGTCTAGTTGGTCTTGGAGC+AGG + chr2.3:29907671-29907690 MS.gene77630:CDS 55.0%
!! GGTTTGCTGCTGCAGACACG+GGG + chr2.3:29907324-29907343 MS.gene77630:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 29907290 29908306 29907290 ID=MS.gene77630
chr2.3 mRNA 29907290 29908306 29907290 ID=MS.gene77630.t1;Parent=MS.gene77630
chr2.3 exon 29907290 29908306 29907290 ID=MS.gene77630.t1.exon1;Parent=MS.gene77630.t1
chr2.3 CDS 29907290 29908306 29907290 ID=cds.MS.gene77630.t1;Parent=MS.gene77630.t1
Gene Sequence

>MS.gene77630

ATGAAAATCTATATAGGCACCCCACCTGTTGAAAGGTTTGCTGCTGCAGACACGGGGAGTGACCTTATTTGGGTACAATGTTCCCCTTGTGAAAATTGTTTTCCACAAGACACTCCTTATTATGATCCAACAAAATCTTCCACTTTCATGGGTTTGTCATGTGACTCACAATCTTGCTCATTACTCCCCAAAGGCTACCATGGATGTGGAAAATCAAATCAGTGTGAGTATATATACACTTATGGTGATAACTCTTACACTATTGGAGATTTGGGTACTGAATCCATTAACTTTGGAGAAAAAGATGTTACATTTCCTAAATCAATTTTTGGATGTGGACATCAAAATGATGTCACATTTAAAAGAAGCCGTAAAGCTACGGGTCTAGTTGGTCTTGGAGCAGGACCACTATCACTAGTTTCACAACTAGGTGATTCAATTGGTCACAAATTTTCCTATTGTTTGATTCCTCCAAATTTAAACTCAAATAGCAAAATGAAATTTGGAGATGAAGCAATTATAAAAGGTAATGGCGTTGTGTCTACTCCCCTCGTAATCAAATCTTCGAATCCTTCTTTTTACTTCCTCAATCTCGAAGGCATCACCGTTGGACAAAAAAGGGCTCAGTCAGGTCGAACTGATGGTAACATAATCATTGATTCAGGTACAACATTGACGTATCTTGAACAAAACTTTTACAATGATTTGGTAGCTTCCGTGAAGGGAGTTATTGGTGTTGAGGAAGTGAATGATCCTCCATCACCATATACTTTTTGCTTCACCTTTGGAGATATAACAAAGTTCCCTAATTTTGTGTTTCATTTGACGGGAGCTGATGTTACCCTTAAACCTCAGAACTTAGTTCTTGATCTTGGCGACAACTCGCATTGCTTGTTGGTCGTACCAACCAATGATCTTTCCATCTTTGGAAATAGAGCACAATTTAATTTTTTAGTAGGGTACGATCTCAAAGGAAAAACAGTTTCCTTTGCTCCAACTGATTGTTCCAAGACCTAA
Protein sequence

>MS.gene77630.t1

MKIYIGTPPVERFAAADTGSDLIWVQCSPCENCFPQDTPYYDPTKSSTFMGLSCDSQSCSLLPKGYHGCGKSNQCEYIYTYGDNSYTIGDLGTESINFGEKDVTFPKSIFGCGHQNDVTFKRSRKATGLVGLGAGPLSLVSQLGDSIGHKFSYCLIPPNLNSNSKMKFGDEAIIKGNGVVSTPLVIKSSNPSFYFLNLEGITVGQKRAQSGRTDGNIIIDSGTTLTYLEQNFYNDLVASVKGVIGVEEVNDPPSPYTFCFTFGDITKFPNFVFHLTGADVTLKPQNLVLDLGDNSHCLLVVPTNDLSIFGNRAQFNFLVGYDLKGKTVSFAPTDCSKT