Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78137.t1 | XP_003606284.1 | 75 | 60 | 9 | 1 | 1 | 60 | 1 | 54 | 1.30E-13 | 85.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78137.t1 | G7JM48 | 75.0 | 60 | 9 | 1 | 1 | 60 | 1 | 54 | 9.4e-14 | 85.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050953 | MS.gene78137 | 0.802329 | 5.91E-49 | -1.69E-46 |
| MS.gene054405 | MS.gene78137 | 0.80074 | 1.25E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78137.t1 | MTR_4g055610 | 75.000 | 60 | 9 | 1 | 1 | 60 | 1 | 54 | 8.99e-23 | 89.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 8 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGAACACCAAGCTCAAT+TGG | 0.331251 | 4.4:-47501979 | MS.gene78137:CDS |
| CTCTTTGTTCTCAAATCAAA+AGG | 0.431632 | 4.4:-47502554 | MS.gene78137:intron |
| TCATGTTCTGCTGTTGAGCT+TGG | 0.442497 | 4.4:+47502644 | None:intergenic |
| CTGCTGTTGAGCTTGGAAGC+AGG | 0.448537 | 4.4:+47502651 | None:intergenic |
| TGCTGTTGAGCTTGGAAGCA+GGG | 0.464847 | 4.4:+47502652 | None:intergenic |
| CTTGAACACCAAGCTCAATT+GGG | 0.466252 | 4.4:-47501978 | MS.gene78137:CDS |
| GACGATTGCCCAATTGAGCT+TGG | 0.544122 | 4.4:+47501970 | None:intergenic |
| TCAACTACGATTTAGAGACA+TGG | 0.691481 | 4.4:-47501949 | MS.gene78137:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTTTATTTTTATTTGTTTT+AGG | - | chr4.4:47502429-47502448 | MS.gene78137:intron | 10.0% |
| !! | GAAAATAATTCTTTGTATTT+GGG | + | chr4.4:47502232-47502251 | None:intergenic | 15.0% |
| !! | TGAAAATAATTCTTTGTATT+TGG | + | chr4.4:47502233-47502252 | None:intergenic | 15.0% |
| !!! | TAGAAAAGATAAATTTTCTT+TGG | + | chr4.4:47502257-47502276 | None:intergenic | 15.0% |
| !! | ATTCCTACTATTTATTATCT+AGG | - | chr4.4:47502589-47502608 | MS.gene78137:CDS | 20.0% |
| !! | TTCCTACTATTTATTATCTA+GGG | - | chr4.4:47502590-47502609 | MS.gene78137:CDS | 20.0% |
| !!! | ATGTTGCTTTTGAATTAATA+AGG | + | chr4.4:47502158-47502177 | None:intergenic | 20.0% |
| !!! | TTTATTTGTTTTAGGTTCAT+GGG | - | chr4.4:47502437-47502456 | MS.gene78137:intron | 20.0% |
| !!! | TTTTATTTGTTTTAGGTTCA+TGG | - | chr4.4:47502436-47502455 | MS.gene78137:intron | 20.0% |
| ! | AACCCTAGATAATAAATAGT+AGG | + | chr4.4:47502595-47502614 | None:intergenic | 25.0% |
| ! | AACTCATACATTTGTGTTAA+TGG | - | chr4.4:47502407-47502426 | MS.gene78137:intron | 25.0% |
| ! | TTAACACAAATGTATGAGTT+AGG | + | chr4.4:47502407-47502426 | None:intergenic | 25.0% |
| !! | GTTAATTGTTATCGACTAAT+TGG | - | chr4.4:47502357-47502376 | MS.gene78137:intron | 25.0% |
| ACATGAAAATAACCATTGGA+AGG | - | chr4.4:47502174-47502193 | MS.gene78137:intron | 30.0% | |
| AGCAACATGAAAATAACCAT+TGG | - | chr4.4:47502170-47502189 | MS.gene78137:intron | 30.0% | |
| ATAAGATAGAGTCATTGTCA+TGG | - | chr4.4:47502310-47502329 | MS.gene78137:intron | 30.0% | |
| CAATGACTCTATCTTATGTT+TGG | + | chr4.4:47502307-47502326 | None:intergenic | 30.0% | |
| CTCTTTGTTCTCAAATCAAA+AGG | - | chr4.4:47502039-47502058 | MS.gene78137:intron | 30.0% | |
| TTTCTTAGTTATTGCAGCTA+TGG | - | chr4.4:47502557-47502576 | MS.gene78137:CDS | 30.0% | |
| ! | AAGCTAGAGTAATTTTTACG+TGG | - | chr4.4:47502204-47502223 | MS.gene78137:intron | 30.0% |
| ! | AGCTAGAGTAATTTTTACGT+GGG | - | chr4.4:47502205-47502224 | MS.gene78137:intron | 30.0% |
| ! | GAACTCGAGAAGTATAAATT+AGG | + | chr4.4:47502072-47502091 | None:intergenic | 30.0% |
| ! | GTTATCGACTAATTGGTTTA+AGG | - | chr4.4:47502364-47502383 | MS.gene78137:intron | 30.0% |
| AAAATAACCATTGGAAGGAG+TGG | - | chr4.4:47502179-47502198 | MS.gene78137:intron | 35.0% | |
| ATGCTTTCACATTACTTGGA+TGG | - | chr4.4:47502520-47502539 | MS.gene78137:intron | 35.0% | |
| CCACAATTGATGTTGTTAAG+TGG | + | chr4.4:47502476-47502495 | None:intergenic | 35.0% | |
| CCACTTAACAACATCAATTG+TGG | - | chr4.4:47502473-47502492 | MS.gene78137:intron | 35.0% | |
| CCTTATGCTTTCACATTACT+TGG | - | chr4.4:47502516-47502535 | MS.gene78137:intron | 35.0% | |
| TCAACTACGATTTAGAGACA+TGG | - | chr4.4:47502644-47502663 | MS.gene78137:CDS | 35.0% | |
| TTTCACATTACTTGGATGGA+CGG | - | chr4.4:47502524-47502543 | MS.gene78137:intron | 35.0% | |
| ! | CCAAGTAATGTGAAAGCATA+AGG | + | chr4.4:47502519-47502538 | None:intergenic | 35.0% |
| ! | GCTAGAGTAATTTTTACGTG+GGG | - | chr4.4:47502206-47502225 | MS.gene78137:intron | 35.0% |
| ! | TGAAAGCATAAGGAAGACTT+AGG | + | chr4.4:47502509-47502528 | None:intergenic | 35.0% |
| AGAGTCATTGTCATGGATGA+TGG | - | chr4.4:47502317-47502336 | MS.gene78137:intron | 40.0% | |
| CACCAAAAAGATGTTGATGC+AGG | - | chr4.4:47502012-47502031 | MS.gene78137:intron | 40.0% | |
| CTTGAACACCAAGCTCAATT+GGG | - | chr4.4:47502615-47502634 | MS.gene78137:CDS | 40.0% | |
| GCAACAAGAACAAGAAACAC+AGG | + | chr4.4:47502127-47502146 | None:intergenic | 40.0% | |
| TCTTGAACACCAAGCTCAAT+TGG | - | chr4.4:47502614-47502633 | MS.gene78137:CDS | 40.0% | |
| ! | CACCTGCATCAACATCTTTT+TGG | + | chr4.4:47502017-47502036 | None:intergenic | 40.0% |
| AAGGAAGACTTAGGAGAGGT+GGG | + | chr4.4:47502500-47502519 | None:intergenic | 45.0% | |
| GCATAAGGAAGACTTAGGAG+AGG | + | chr4.4:47502504-47502523 | None:intergenic | 45.0% | |
| TAAGGAAGACTTAGGAGAGG+TGG | + | chr4.4:47502501-47502520 | None:intergenic | 45.0% | |
| TCATGTTCTGCTGTTGAGCT+TGG | + | chr4.4:47501952-47501971 | None:intergenic | 45.0% | |
| ACATTACTTGGATGGACGGC+TGG | - | chr4.4:47502528-47502547 | MS.gene78137:intron | 50.0% | |
| AGGAAGACTTAGGAGAGGTG+GGG | + | chr4.4:47502499-47502518 | None:intergenic | 50.0% | |
| GACGATTGCCCAATTGAGCT+TGG | + | chr4.4:47502626-47502645 | None:intergenic | 50.0% | |
| TGCTGTTGAGCTTGGAAGCA+GGG | + | chr4.4:47501944-47501963 | None:intergenic | 50.0% | |
| TTAGGTTCATGGGTGCACTG+TGG | - | chr4.4:47502447-47502466 | MS.gene78137:intron | 50.0% | |
| AGCTTCGCCACTCCTTCCAA+TGG | + | chr4.4:47502189-47502208 | None:intergenic | 55.0% | |
| CTGCTGTTGAGCTTGGAAGC+AGG | + | chr4.4:47501945-47501964 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 47501934 | 47502681 | 47501934 | ID=MS.gene78137 |
| chr4.4 | mRNA | 47501934 | 47502681 | 47501934 | ID=MS.gene78137.t1;Parent=MS.gene78137 |
| chr4.4 | exon | 47502555 | 47502681 | 47502555 | ID=MS.gene78137.t1.exon1;Parent=MS.gene78137.t1 |
| chr4.4 | CDS | 47502555 | 47502681 | 47502555 | ID=cds.MS.gene78137.t1;Parent=MS.gene78137.t1 |
| chr4.4 | exon | 47501934 | 47502004 | 47501934 | ID=MS.gene78137.t1.exon2;Parent=MS.gene78137.t1 |
| chr4.4 | CDS | 47501934 | 47502004 | 47501934 | ID=cds.MS.gene78137.t1;Parent=MS.gene78137.t1 |
| Gene Sequence |
| Protein sequence |
