Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78181.t1 | XP_003595645.1 | 96.6 | 59 | 2 | 0 | 1 | 59 | 1 | 59 | 1.30E-23 | 118.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78181.t1 | O23882 | 88.1 | 59 | 7 | 0 | 1 | 59 | 1 | 59 | 6.5e-24 | 110.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78181.t1 | G7IQL2 | 96.6 | 59 | 2 | 0 | 1 | 59 | 1 | 59 | 9.4e-24 | 118.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene78181 | 0.808685 | 2.74E-50 | -1.69E-46 |
| MS.gene053887 | MS.gene78181 | 0.803331 | 3.67E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78181.t1 | MTR_2g058470 | 96.610 | 59 | 2 | 0 | 1 | 59 | 1 | 59 | 1.12e-35 | 123 |
| MS.gene78181.t1 | MTR_5g007760 | 89.831 | 59 | 6 | 0 | 1 | 59 | 1 | 59 | 1.49e-33 | 117 |
| MS.gene78181.t1 | MTR_5g007717 | 86.441 | 59 | 8 | 0 | 1 | 59 | 1 | 59 | 1.37e-32 | 115 |
| MS.gene78181.t1 | MTR_7g016820 | 86.441 | 59 | 8 | 0 | 1 | 59 | 1 | 59 | 1.88e-32 | 114 |
| MS.gene78181.t1 | MTR_5g007713 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1 | 59 | 3.94e-32 | 114 |
| MS.gene78181.t1 | MTR_7g016720 | 86.441 | 59 | 8 | 0 | 1 | 59 | 1 | 59 | 5.68e-32 | 113 |
| MS.gene78181.t1 | MTR_7g016780 | 86.441 | 59 | 8 | 0 | 1 | 59 | 1 | 59 | 6.71e-32 | 113 |
| MS.gene78181.t1 | MTR_7g016700 | 86.441 | 59 | 8 | 0 | 1 | 59 | 1 | 59 | 7.07e-32 | 113 |
| MS.gene78181.t1 | MTR_5g007730 | 86.441 | 59 | 8 | 0 | 1 | 59 | 1 | 59 | 8.10e-32 | 113 |
| MS.gene78181.t1 | MTR_1g098140 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1 | 59 | 3.41e-31 | 111 |
| MS.gene78181.t1 | MTR_7g016800 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1 | 59 | 3.52e-31 | 111 |
| MS.gene78181.t1 | MTR_1g097935 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1 | 59 | 4.72e-31 | 110 |
| MS.gene78181.t1 | MTR_1g097910 | 84.746 | 59 | 9 | 0 | 1 | 59 | 1 | 59 | 4.72e-31 | 110 |
| MS.gene78181.t1 | MTR_3g086260 | 81.356 | 59 | 11 | 0 | 1 | 59 | 1 | 59 | 3.82e-30 | 108 |
| MS.gene78181.t1 | MTR_3g083920 | 77.966 | 59 | 13 | 0 | 1 | 59 | 1 | 59 | 8.49e-30 | 107 |
| MS.gene78181.t1 | MTR_3g083910 | 74.576 | 59 | 15 | 0 | 1 | 59 | 1 | 59 | 4.40e-28 | 103 |
| MS.gene78181.t1 | MTR_7g084300 | 66.102 | 59 | 20 | 0 | 1 | 59 | 1 | 59 | 8.25e-24 | 91.7 |
| MS.gene78181.t1 | MTR_3g088675 | 61.017 | 59 | 23 | 0 | 1 | 59 | 1 | 59 | 3.25e-19 | 79.0 |
| MS.gene78181.t1 | MTR_5g007723 | 93.103 | 29 | 2 | 0 | 12 | 40 | 32 | 60 | 6.91e-14 | 63.9 |
| MS.gene78181.t1 | MTR_3g088670 | 57.692 | 52 | 20 | 1 | 10 | 59 | 2 | 53 | 6.73e-12 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78181.t1 | AT5G13930 | 67.857 | 56 | 18 | 0 | 4 | 59 | 9 | 64 | 1.66e-22 | 88.2 |
Find 13 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAAGGCCCTGCAACCATTT+TGG | 0.230031 | 2.3:+35949381 | MS.gene78181:CDS |
| TGCAGTGCCAATGGCCAAAA+TGG | 0.341928 | 2.3:-35949395 | None:intergenic |
| CCAATGGCCAAAATGGTTGC+AGG | 0.375325 | 2.3:-35949388 | None:intergenic |
| GTGACTCTAAAGTAGAAATC+AGG | 0.439149 | 2.3:-35949451 | None:intergenic |
| GTGCTTTGTTCAAAACAGTT+TGG | 0.466477 | 2.3:-35949424 | None:intergenic |
| CTGAAATTCGCAAGGCTCAA+AGG | 0.467872 | 2.3:+35949356 | MS.gene78181:CDS |
| TGAAATTCGCAAGGCTCAAA+GGG | 0.479464 | 2.3:+35949357 | MS.gene78181:CDS |
| CTTTGTTCAAAACAGTTTGG+TGG | 0.522337 | 2.3:-35949421 | None:intergenic |
| AACAAATATCAAGATGATGA+AGG | 0.528056 | 2.3:+35949330 | None:intergenic |
| CGCAAGGCTCAAAGGGCAGA+AGG | 0.557567 | 2.3:+35949364 | MS.gene78181:CDS |
| TGGTGGATTTGCAGTGCCAA+TGG | 0.565777 | 2.3:-35949404 | None:intergenic |
| CAATGGCCAAAATGGTTGCA+GGG | 0.611325 | 2.3:-35949387 | None:intergenic |
| GAAGGTGTCTGAAATTCGCA+AGG | 0.636559 | 2.3:+35949348 | MS.gene78181:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGATTAAAAAAGAAAAA+TGG | - | chr2.3:35949571-35949590 | None:intergenic | 10.0% |
| CTTTGTTCAAAACAGTTTGG+TGG | - | chr2.3:35949424-35949443 | None:intergenic | 35.0% | |
| ! | GTGCTTTGTTCAAAACAGTT+TGG | - | chr2.3:35949427-35949446 | None:intergenic | 35.0% |
| !! | GTGACTCTAAAGTAGAAATC+AGG | - | chr2.3:35949454-35949473 | None:intergenic | 35.0% |
| TGAAATTCGCAAGGCTCAAA+GGG | + | chr2.3:35949357-35949376 | MS.gene78181:CDS | 40.0% | |
| CAATGGCCAAAATGGTTGCA+GGG | - | chr2.3:35949390-35949409 | None:intergenic | 45.0% | |
| CTGAAATTCGCAAGGCTCAA+AGG | + | chr2.3:35949356-35949375 | MS.gene78181:CDS | 45.0% | |
| !! | GAAGGTGTCTGAAATTCGCA+AGG | + | chr2.3:35949348-35949367 | MS.gene78181:CDS | 45.0% |
| CCAATGGCCAAAATGGTTGC+AGG | - | chr2.3:35949391-35949410 | None:intergenic | 50.0% | |
| TGCAGTGCCAATGGCCAAAA+TGG | - | chr2.3:35949398-35949417 | None:intergenic | 50.0% | |
| ! | AGAAGGCCCTGCAACCATTT+TGG | + | chr2.3:35949381-35949400 | MS.gene78181:CDS | 50.0% |
| !! | CCTGCAACCATTTTGGCCAT+TGG | + | chr2.3:35949388-35949407 | MS.gene78181:CDS | 50.0% |
| !! | TGGTGGATTTGCAGTGCCAA+TGG | - | chr2.3:35949407-35949426 | None:intergenic | 50.0% |
| CGCAAGGCTCAAAGGGCAGA+AGG | + | chr2.3:35949364-35949383 | MS.gene78181:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.3 | gene | 35949343 | 35949625 | 35949343 | ID=MS.gene78181 |
| chr2.3 | mRNA | 35949343 | 35949625 | 35949343 | ID=MS.gene78181.t1;Parent=MS.gene78181 |
| chr2.3 | exon | 35949343 | 35949520 | 35949343 | ID=MS.gene78181.t1.exon1;Parent=MS.gene78181.t1 |
| chr2.3 | CDS | 35949343 | 35949520 | 35949343 | ID=cds.MS.gene78181.t1;Parent=MS.gene78181.t1 |
| chr2.3 | exon | 35949618 | 35949625 | 35949618 | ID=MS.gene78181.t1.exon2;Parent=MS.gene78181.t1 |
| chr2.3 | CDS | 35949618 | 35949625 | 35949618 | ID=cds.MS.gene78181.t1;Parent=MS.gene78181.t1 |
| Gene Sequence |
| Protein sequence |
