Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78810.t1 | KEH29073.1 | 87.7 | 146 | 8 | 1 | 1 | 146 | 1 | 136 | 6.40E-45 | 190.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78810.t1 | A0A072UIZ3 | 87.7 | 146 | 8 | 1 | 1 | 146 | 1 | 136 | 4.6e-45 | 190.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052463 | MS.gene78810 | 0.800184 | 1.62E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78810.t1 | MTR_4g021870 | 86.986 | 146 | 9 | 1 | 1 | 146 | 1 | 136 | 2.58e-76 | 223 |
| MS.gene78810.t1 | MTR_7g060440 | 47.727 | 88 | 35 | 5 | 68 | 146 | 102 | 187 | 1.68e-13 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGGACATTGCTTTGGTATT+TGG | 0.141906 | 4.2:+80706285 | None:intergenic |
| CAGAGAAGATGGTGATATTA+AGG | 0.229523 | 4.2:-80706441 | MS.gene78810:CDS |
| CTATTCTCTTCGAGCTTTCT+TGG | 0.239268 | 4.2:+80706130 | None:intergenic |
| AACATCATCATCAAGAGAAT+TGG | 0.348667 | 4.2:-80706366 | MS.gene78810:CDS |
| ATATTAAACATCAACAATAT+TGG | 0.383707 | 4.2:+80706470 | None:intergenic |
| GGTGCAAGTGGACATTGCTT+TGG | 0.434789 | 4.2:+80706278 | None:intergenic |
| GTGTAGCAACAACAATTTGA+AGG | 0.448094 | 4.2:-80706504 | MS.gene78810:CDS |
| ATTCTATTATCAAAATGAGT+TGG | 0.466175 | 4.2:+80706308 | None:intergenic |
| AATATTCAAGACAGAGAAGA+TGG | 0.485196 | 4.2:-80706452 | MS.gene78810:CDS |
| AATGAAGAAGATGAAGATGA+TGG | 0.487738 | 4.2:-80706341 | MS.gene78810:CDS |
| CCAAACAACATATGAAAAGA+AGG | 0.494232 | 4.2:-80706247 | MS.gene78810:CDS |
| ACTACTTGATGTGTCAAGAG+AGG | 0.671924 | 4.2:-80706207 | MS.gene78810:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTAAACATCAACAATAT+TGG | + | chr4.2:80706211-80706230 | None:intergenic | 15.0% |
| !! | ATTCTATTATCAAAATGAGT+TGG | + | chr4.2:80706373-80706392 | None:intergenic | 20.0% |
| !! | TAAGCAAAAATTAGATTTAG+AGG | - | chr4.2:80706282-80706301 | MS.gene78810:CDS | 20.0% |
| !!! | AAAATGTTTTGTTCTTGATT+TGG | + | chr4.2:80706510-80706529 | None:intergenic | 20.0% |
| !!! | ATATGTTGTTTGGTTTTTCT+TGG | + | chr4.2:80706424-80706443 | None:intergenic | 25.0% |
| AACATCATCATCAAGAGAAT+TGG | - | chr4.2:80706312-80706331 | MS.gene78810:CDS | 30.0% | |
| AATATTCAAGACAGAGAAGA+TGG | - | chr4.2:80706226-80706245 | MS.gene78810:CDS | 30.0% | |
| AATGAAGAAGATGAAGATGA+TGG | - | chr4.2:80706337-80706356 | MS.gene78810:CDS | 30.0% | |
| CCAAACAACATATGAAAAGA+AGG | - | chr4.2:80706431-80706450 | MS.gene78810:CDS | 30.0% | |
| ! | CCTTCTTTTCATATGTTGTT+TGG | + | chr4.2:80706434-80706453 | None:intergenic | 30.0% |
| CAGAGAAGATGGTGATATTA+AGG | - | chr4.2:80706237-80706256 | MS.gene78810:CDS | 35.0% | |
| GTGTAGCAACAACAATTTGA+AGG | - | chr4.2:80706174-80706193 | MS.gene78810:CDS | 35.0% | |
| ACTACTTGATGTGTCAAGAG+AGG | - | chr4.2:80706471-80706490 | MS.gene78810:CDS | 40.0% | |
| CTATTCTCTTCGAGCTTTCT+TGG | + | chr4.2:80706551-80706570 | None:intergenic | 40.0% | |
| GTGGACATTGCTTTGGTATT+TGG | + | chr4.2:80706396-80706415 | None:intergenic | 40.0% | |
| !! | TTGGTTTTTCTTGGTGCAAG+TGG | + | chr4.2:80706415-80706434 | None:intergenic | 40.0% |
| GGTGCAAGTGGACATTGCTT+TGG | + | chr4.2:80706403-80706422 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 80706130 | 80706570 | 80706130 | ID=MS.gene78810 |
| chr4.2 | mRNA | 80706130 | 80706570 | 80706130 | ID=MS.gene78810.t1;Parent=MS.gene78810 |
| chr4.2 | exon | 80706130 | 80706570 | 80706130 | ID=MS.gene78810.t1.exon1;Parent=MS.gene78810.t1 |
| chr4.2 | CDS | 80706130 | 80706570 | 80706130 | ID=cds.MS.gene78810.t1;Parent=MS.gene78810.t1 |
| Gene Sequence |
| Protein sequence |
