Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79079.t1 | RHN69325.1 | 95.8 | 616 | 26 | 0 | 1 | 616 | 1 | 616 | 2.60E-273 | 951 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79079.t1 | Q0WPZ7 | 45.8 | 607 | 273 | 10 | 15 | 609 | 21 | 583 | 1.2e-80 | 302.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79079.t1 | G7J344 | 95.8 | 616 | 26 | 0 | 1 | 616 | 4 | 619 | 1.9e-273 | 951.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene79079 | MS.gene07517 | PPI |
| MS.gene79079 | MS.gene065809 | PPI |
| MS.gene79079 | MS.gene004979 | PPI |
| MS.gene79079 | MS.gene86831 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79079.t1 | MTR_3g086460 | 95.942 | 616 | 25 | 0 | 1 | 616 | 4 | 619 | 0.0 | 1217 |
| MS.gene79079.t1 | MTR_7g034350 | 52.941 | 187 | 44 | 3 | 431 | 616 | 4 | 147 | 6.83e-55 | 184 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79079.t1 | AT1G13120 | 42.414 | 613 | 290 | 15 | 15 | 611 | 21 | 586 | 9.48e-144 | 430 |
| MS.gene79079.t1 | AT4G05523 | 31.731 | 208 | 104 | 8 | 15 | 213 | 21 | 199 | 9.71e-20 | 89.0 |
Find 118 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCCGGAATATAAAGCAAATA+AGG | 0.196341 | 3.1:-69337173 | MS.gene79079:CDS |
| CAAGAGAAAGGTTGCTTTGC+AGG | 0.219008 | 3.1:-69338731 | MS.gene79079:CDS |
| TTCCATTGCTGCCTTTGTTT+TGG | 0.224984 | 3.1:+69338309 | None:intergenic |
| CCTTGGTGTCAGTATTTAAT+AGG | 0.247209 | 3.1:+69338200 | None:intergenic |
| TGTTGAGATATTAATTAAAA+AGG | 0.253115 | 3.1:-69336418 | MS.gene79079:intron |
| CCCTTATTTGCTTTATATTC+CGG | 0.260197 | 3.1:+69337172 | None:intergenic |
| GACTGCAGGTTCCTTCCTTC+TGG | 0.268189 | 3.1:+69330140 | None:intergenic |
| CCTTATTTGCTTTATATTCC+GGG | 0.310245 | 3.1:+69337173 | None:intergenic |
| TTGTAGGGGAGCTATGAAAT+TGG | 0.312211 | 3.1:-69340448 | None:intergenic |
| TACTTCCTCGGCTTTAAGTT+TGG | 0.326776 | 3.1:+69338375 | None:intergenic |
| AAAGCACCTGGTTTACAAAA+AGG | 0.349016 | 3.1:-69331125 | MS.gene79079:intron |
| CAAAGAAACAAGGCAGGAAT+TGG | 0.357926 | 3.1:-69339517 | MS.gene79079:CDS |
| GGTGTACGGTGCACTGGTTC+AGG | 0.362844 | 3.1:-69330642 | MS.gene79079:intron |
| AGCAAAATATCCATTACATA+TGG | 0.370046 | 3.1:+69331184 | None:intergenic |
| CCGGAATATAAAGCAAATAA+GGG | 0.387902 | 3.1:-69337172 | MS.gene79079:CDS |
| CTGCAAAGCAACCTTTCTCT+TGG | 0.394273 | 3.1:+69338732 | None:intergenic |
| CGTCTCCGAGCTCAATGCTT+TGG | 0.394800 | 3.1:-69340367 | MS.gene79079:CDS |
| AAGAAATTGAGAAAGGTAGA+AGG | 0.400427 | 3.1:-69340110 | MS.gene79079:CDS |
| TTCCGATGTTGACTCTGCTT+TGG | 0.402079 | 3.1:-69339864 | MS.gene79079:CDS |
| GCAGTAGTCTTCGCTGAATC+TGG | 0.404397 | 3.1:+69330206 | None:intergenic |
| TTGCTGCTTCTGATCCTGAT+TGG | 0.416301 | 3.1:-69340404 | MS.gene79079:CDS |
| TCGCTGTCTGTCTCATACTC+AGG | 0.418770 | 3.1:+69340072 | None:intergenic |
| TCCTTCCTTCTGGCACTTGA+AGG | 0.419682 | 3.1:+69330150 | None:intergenic |
| CCTCCCCAACTTTATTCATC+AGG | 0.419766 | 3.1:+69339828 | None:intergenic |
| CTATTTGTGATCTGAGTTCA+CGG | 0.419778 | 3.1:+69338794 | None:intergenic |
| AATAAAGTTGGGGAGGTAGA+AGG | 0.421366 | 3.1:-69339821 | MS.gene79079:CDS |
| TGAATTTCTCAACTCGAAGA+AGG | 0.425487 | 3.1:+69339538 | None:intergenic |
| CTACTTTGCCCGCAGATGGC+TGG | 0.429531 | 3.1:-69330323 | MS.gene79079:intron |
| CTTACCTGATGAATAAAGTT+GGG | 0.433506 | 3.1:-69339832 | MS.gene79079:CDS |
| CGCATGGCTTGCAAGAAGGT+TGG | 0.436683 | 3.1:-69330483 | MS.gene79079:CDS |
| ATTGCAAAGGGAGCACTAGC+AGG | 0.440136 | 3.1:+69331398 | None:intergenic |
| CATTGTTCTCATTACATCTC+AGG | 0.442576 | 3.1:-69331366 | MS.gene79079:intron |
| GCCACGAGAGTGATATTTCC+CGG | 0.447718 | 3.1:-69337191 | MS.gene79079:CDS |
| CGCTGTCTGTCTCATACTCA+GGG | 0.448994 | 3.1:+69340073 | None:intergenic |
| GGAAATATCACTCTCGTGGC+GGG | 0.451213 | 3.1:+69337194 | None:intergenic |
| TGTAGGGGAGCTATGAAATT+GGG | 0.453562 | 3.1:-69340447 | None:intergenic |
| AGCTATGAAATTGGGTCGAC+TGG | 0.455057 | 3.1:-69340439 | None:intergenic |
| CCAACTTTATTCATCAGGTA+AGG | 0.459982 | 3.1:+69339833 | None:intergenic |
| GCATGGCTTGCAAGAAGGTT+GGG | 0.460900 | 3.1:-69330482 | MS.gene79079:CDS |
| ACAGCTGTATATTGATTAGC+TGG | 0.468190 | 3.1:+69330430 | None:intergenic |
| AAAACCCATACCGGATTCCT+TGG | 0.468656 | 3.1:+69338183 | None:intergenic |
| GGGGCATAAGAGACAATGTT+AGG | 0.469707 | 3.1:-69337152 | MS.gene79079:intron |
| AAGGTTGGGCGTGGCTTGCA+AGG | 0.480309 | 3.1:-69330468 | MS.gene79079:CDS |
| CCTTACCTGATGAATAAAGT+TGG | 0.481739 | 3.1:-69339833 | MS.gene79079:CDS |
| TGACAAAGCGTTAGCAGTAT+CGG | 0.487789 | 3.1:-69340040 | MS.gene79079:CDS |
| GAGGCATACCTCAGAAGTAT+TGG | 0.492547 | 3.1:-69330734 | MS.gene79079:CDS |
| ATCTCAACACTGAATGATTG+AGG | 0.492927 | 3.1:+69336432 | None:intergenic |
| TCGAGAAGACAATGGAAAGT+TGG | 0.493098 | 3.1:-69330708 | MS.gene79079:CDS |
| TGAATGATTGAGGGCATTGA+CGG | 0.503300 | 3.1:+69336442 | None:intergenic |
| GGAAGTAAAACAAGCTGCAT+TGG | 0.503573 | 3.1:-69338359 | MS.gene79079:CDS |
| AATACTGACACCAAGGAATC+CGG | 0.505188 | 3.1:-69338193 | MS.gene79079:intron |
| GCAAAATATCCATTACATAT+GGG | 0.508499 | 3.1:+69331185 | None:intergenic |
| TTCATCAAACTACTTCATCA+AGG | 0.511278 | 3.1:-69340302 | MS.gene79079:intron |
| TATGAAGGTGTACGGTGCAC+TGG | 0.512093 | 3.1:-69330648 | MS.gene79079:CDS |
| AGCCAAAACAAAGGCAGCAA+TGG | 0.513768 | 3.1:-69338311 | MS.gene79079:CDS |
| GTCTATCTTTCAATCAAAAG+AGG | 0.513894 | 3.1:-69330753 | MS.gene79079:CDS |
| GGTGCTTTGGGACGGTGTAA+AGG | 0.520108 | 3.1:+69331140 | None:intergenic |
| TAGAAATAAGATCTCAGCGT+TGG | 0.520586 | 3.1:-69339601 | MS.gene79079:CDS |
| TCAGATCACAAATAGAAGAA+AGG | 0.525142 | 3.1:-69338786 | MS.gene79079:CDS |
| AGAGCATCGAAACTCCAATC+AGG | 0.527876 | 3.1:+69340390 | None:intergenic |
| AGTGGGAAAGAAATTGAGAA+AGG | 0.533181 | 3.1:-69340117 | MS.gene79079:CDS |
| AATCCGCATGGCTTGCAAGA+AGG | 0.533446 | 3.1:-69330487 | MS.gene79079:CDS |
| CTTCAAATATAGAGCATGGT+AGG | 0.534137 | 3.1:-69337547 | MS.gene79079:CDS |
| TTACACCGTCCCAAAGCACC+TGG | 0.537516 | 3.1:-69331137 | MS.gene79079:CDS |
| GAATTTCTCAACTCGAAGAA+GGG | 0.543891 | 3.1:+69339539 | None:intergenic |
| AAAGGCTAAAGCTGAAGCAG+AGG | 0.545258 | 3.1:-69338689 | MS.gene79079:intron |
| ATGACATTGGCAATTGCAAA+GGG | 0.545913 | 3.1:+69331386 | None:intergenic |
| TTAGGGTTAACTTATTCAGT+GGG | 0.546979 | 3.1:-69340134 | MS.gene79079:intron |
| AGCACTAGCAGGGCTTGCAC+AGG | 0.547376 | 3.1:+69331409 | None:intergenic |
| TCTCAACACTGAATGATTGA+GGG | 0.548370 | 3.1:+69336433 | None:intergenic |
| GTATGAAGAGGCCAAGAGAA+AGG | 0.551262 | 3.1:-69338743 | MS.gene79079:CDS |
| GTGATAACGTATTTGACTGC+AGG | 0.554583 | 3.1:+69330126 | None:intergenic |
| TTTAGGGTTAACTTATTCAG+TGG | 0.560055 | 3.1:-69340135 | MS.gene79079:intron |
| GTTTGATGAAAATGCAAAGG+TGG | 0.561720 | 3.1:+69340315 | None:intergenic |
| AGGTGGAGTGGAAGAATTAG+CGG | 0.565983 | 3.1:+69340332 | None:intergenic |
| GCGGCTAGAATCATATATGA+AGG | 0.567325 | 3.1:-69330663 | MS.gene79079:CDS |
| GCTTGCAAGAAGGTTGGGCG+TGG | 0.571452 | 3.1:-69330477 | MS.gene79079:CDS |
| GAAATTCAAAGAAACAAGGC+AGG | 0.576260 | 3.1:-69339523 | MS.gene79079:CDS |
| GTAAACCAGGTGCTTTGGGA+CGG | 0.576994 | 3.1:+69331132 | None:intergenic |
| TCCTTCAAGTGCCAGAAGGA+AGG | 0.577361 | 3.1:-69330151 | MS.gene79079:CDS |
| ATGAAAATGCAAAGGTGGAG+TGG | 0.582161 | 3.1:+69340320 | None:intergenic |
| ACGACAGGTCCCATATGTAA+TGG | 0.582923 | 3.1:-69331194 | MS.gene79079:intron |
| GAATCATATATGAAGGTGTA+CGG | 0.586014 | 3.1:-69330656 | MS.gene79079:CDS |
| AATGACATTGGCAATTGCAA+AGG | 0.588611 | 3.1:+69331385 | None:intergenic |
| AAGTGATGCAGCGTATGAAG+AGG | 0.595948 | 3.1:-69338755 | MS.gene79079:CDS |
| ATTCTCCAAAGCATTGAGCT+CGG | 0.597981 | 3.1:+69340362 | None:intergenic |
| TAGAACTTGATCTTACAACT+TGG | 0.600852 | 3.1:+69337492 | None:intergenic |
| CTCGATAACCAATACTTCTG+AGG | 0.601452 | 3.1:+69330726 | None:intergenic |
| TGTAATGAGAACAATGACAT+TGG | 0.604848 | 3.1:+69331373 | None:intergenic |
| TTGCAAAGGGAGCACTAGCA+GGG | 0.607083 | 3.1:+69331399 | None:intergenic |
| AGTATTTAATAGGCTTGATG+CGG | 0.608274 | 3.1:+69338210 | None:intergenic |
| CCTGATGAATAAAGTTGGGG+AGG | 0.613663 | 3.1:-69339828 | MS.gene79079:CDS |
| GTACAGAAGATTATTTAAAG+CGG | 0.615401 | 3.1:-69330682 | MS.gene79079:CDS |
| CAGTTCCTTCAAGTGCCAGA+AGG | 0.616857 | 3.1:-69330155 | MS.gene79079:CDS |
| CCGAACATTCAAAATCCGCA+TGG | 0.620982 | 3.1:-69330499 | MS.gene79079:CDS |
| GAGAAAGGTTGCTTTGCAGG+AGG | 0.625015 | 3.1:-69338728 | MS.gene79079:CDS |
| GAAGACTACTGCATACATGC+AGG | 0.626530 | 3.1:-69330195 | MS.gene79079:CDS |
| ATTGGTTATCGAGAAGACAA+TGG | 0.628835 | 3.1:-69330716 | MS.gene79079:CDS |
| AGTGCTTCAAATATAGAGCA+TGG | 0.630506 | 3.1:-69337551 | MS.gene79079:CDS |
| TTACCTGATGAATAAAGTTG+GGG | 0.630676 | 3.1:-69339831 | MS.gene79079:CDS |
| CTGTCTCATACTCAGGGGCA+CGG | 0.632727 | 3.1:+69340079 | None:intergenic |
| GCGTTAGCAGTATCGGACAC+TGG | 0.634293 | 3.1:-69340033 | MS.gene79079:CDS |
| CGGAATATAAAGCAAATAAG+GGG | 0.635811 | 3.1:-69337171 | MS.gene79079:CDS |
| GCAGGCCAAACTTAAAGCCG+AGG | 0.640019 | 3.1:-69338380 | MS.gene79079:intron |
| GGGAAATATCACTCTCGTGG+CGG | 0.640943 | 3.1:+69337193 | None:intergenic |
| CCTATTAAATACTGACACCA+AGG | 0.642682 | 3.1:-69338200 | MS.gene79079:CDS |
| TCCGGGAAATATCACTCTCG+TGG | 0.645571 | 3.1:+69337190 | None:intergenic |
| CAACCTTCTTGCAAGCCATG+CGG | 0.652087 | 3.1:+69330484 | None:intergenic |
| AACACACGATCATCAACTGA+GGG | 0.653093 | 3.1:-69339783 | MS.gene79079:CDS |
| CTTGTGATGAAATTTATCTG+AGG | 0.654595 | 3.1:-69339999 | MS.gene79079:intron |
| GTAGTTTGATGAAAATGCAA+AGG | 0.657587 | 3.1:+69340312 | None:intergenic |
| TGTAAGATCAAGTTCTACAC+AGG | 0.659828 | 3.1:-69337486 | MS.gene79079:intron |
| TTGAGAAATTCAAAGAAACA+AGG | 0.661129 | 3.1:-69339527 | MS.gene79079:CDS |
| AGTAATGGAAGCCAAAACAA+AGG | 0.684556 | 3.1:-69338320 | MS.gene79079:CDS |
| TTCCAAAGCAGAGTCAACAT+CGG | 0.691363 | 3.1:+69339862 | None:intergenic |
| TAACACACGATCATCAACTG+AGG | 0.709226 | 3.1:-69339784 | MS.gene79079:CDS |
| TGAGTATGAGACAGACAGCG+AGG | 0.732630 | 3.1:-69340070 | MS.gene79079:CDS |
| GCTGTCTGTCTCATACTCAG+GGG | 0.738330 | 3.1:+69340074 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 69330109 | 69340457 | 69330109 | ID=MS.gene79079 |
| chr3.1 | mRNA | 69330109 | 69340457 | 69330109 | ID=MS.gene79079.t1;Parent=MS.gene79079 |
| chr3.1 | exon | 69340303 | 69340457 | 69340303 | ID=MS.gene79079.t1.exon1;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69340303 | 69340457 | 69340303 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69340000 | 69340152 | 69340000 | ID=MS.gene79079.t1.exon2;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69340000 | 69340152 | 69340000 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69339778 | 69339898 | 69339778 | ID=MS.gene79079.t1.exon3;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69339778 | 69339898 | 69339778 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69339483 | 69339631 | 69339483 | ID=MS.gene79079.t1.exon4;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69339483 | 69339631 | 69339483 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69338690 | 69338858 | 69338690 | ID=MS.gene79079.t1.exon5;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69338690 | 69338858 | 69338690 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69338194 | 69338398 | 69338194 | ID=MS.gene79079.t1.exon6;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69338194 | 69338398 | 69338194 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69337487 | 69337596 | 69337487 | ID=MS.gene79079.t1.exon7;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69337487 | 69337596 | 69337487 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69337153 | 69337223 | 69337153 | ID=MS.gene79079.t1.exon8;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69337153 | 69337223 | 69337153 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69336419 | 69336500 | 69336419 | ID=MS.gene79079.t1.exon9;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69336419 | 69336500 | 69336419 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69331367 | 69331441 | 69331367 | ID=MS.gene79079.t1.exon10;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69331367 | 69331441 | 69331367 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69331126 | 69331209 | 69331126 | ID=MS.gene79079.t1.exon11;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69331126 | 69331209 | 69331126 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69330643 | 69330774 | 69330643 | ID=MS.gene79079.t1.exon12;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69330643 | 69330774 | 69330643 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69330408 | 69330530 | 69330408 | ID=MS.gene79079.t1.exon13;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69330408 | 69330530 | 69330408 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| chr3.1 | exon | 69330109 | 69330330 | 69330109 | ID=MS.gene79079.t1.exon14;Parent=MS.gene79079.t1 |
| chr3.1 | CDS | 69330109 | 69330330 | 69330109 | ID=cds.MS.gene79079.t1;Parent=MS.gene79079.t1 |
| Gene Sequence |
| Protein sequence |
