Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79469.t1 | XP_013455961.1 | 96.7 | 61 | 2 | 0 | 78 | 138 | 168 | 228 | 1.50E-21 | 112.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79469.t1 | Q9FJH5 | 68.4 | 57 | 18 | 0 | 74 | 130 | 135 | 191 | 1.2e-11 | 71.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79469.t1 | A0A072UJX7 | 96.7 | 61 | 2 | 0 | 78 | 138 | 168 | 228 | 1.1e-21 | 112.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052609 | MS.gene79469 | 0.802607 | 5.18E-49 | -1.69E-46 |
| MS.gene053581 | MS.gene79469 | 0.802374 | 5.78E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79469.t1 | MTR_4g057765 | 93.805 | 113 | 6 | 1 | 27 | 139 | 118 | 229 | 3.33e-63 | 197 |
| MS.gene79469.t1 | MTR_2g095480 | 54.264 | 129 | 56 | 2 | 10 | 137 | 99 | 225 | 1.76e-30 | 113 |
| MS.gene79469.t1 | MTR_7g101930 | 45.455 | 132 | 53 | 3 | 30 | 142 | 68 | 199 | 1.69e-17 | 78.2 |
| MS.gene79469.t1 | MTR_7g101930 | 40.625 | 96 | 50 | 2 | 60 | 152 | 5 | 96 | 7.39e-13 | 65.5 |
| MS.gene79469.t1 | MTR_1g063210 | 54.412 | 68 | 31 | 0 | 72 | 139 | 135 | 202 | 2.54e-17 | 77.8 |
| MS.gene79469.t1 | MTR_0041s0140 | 45.455 | 132 | 53 | 3 | 30 | 142 | 68 | 199 | 4.10e-17 | 76.3 |
| MS.gene79469.t1 | MTR_0041s0140 | 40.625 | 96 | 50 | 2 | 60 | 152 | 5 | 96 | 1.86e-12 | 63.5 |
| MS.gene79469.t1 | MTR_3g435930 | 36.449 | 107 | 59 | 1 | 32 | 138 | 85 | 182 | 2.67e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79469.t1 | AT5G60800 | 67.568 | 74 | 24 | 0 | 71 | 144 | 132 | 205 | 1.25e-23 | 94.0 |
| MS.gene79469.t1 | AT5G60800 | 67.568 | 74 | 24 | 0 | 71 | 144 | 132 | 205 | 1.60e-23 | 94.0 |
| MS.gene79469.t1 | AT5G03380 | 39.041 | 146 | 56 | 3 | 30 | 142 | 52 | 197 | 1.42e-18 | 81.6 |
| MS.gene79469.t1 | AT5G03380 | 39.041 | 146 | 56 | 3 | 30 | 142 | 79 | 224 | 1.63e-18 | 81.6 |
| MS.gene79469.t1 | AT2G28090 | 45.192 | 104 | 46 | 2 | 37 | 137 | 92 | 187 | 7.24e-16 | 72.8 |
| MS.gene79469.t1 | AT3G02960 | 34.862 | 109 | 57 | 2 | 32 | 137 | 94 | 191 | 2.95e-14 | 68.6 |
| MS.gene79469.t1 | AT2G36950 | 42.857 | 126 | 61 | 2 | 31 | 147 | 106 | 229 | 2.53e-13 | 67.0 |
Find 38 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTTGTTGTTATCCTTCT+TGG | 0.114690 | 4.1:-6270939 | None:intergenic |
| CCACCTTCCTTGTCTTTCTT+TGG | 0.170033 | 4.1:-6271416 | None:intergenic |
| GAAGGAAATGAAGGAGGAGG+TGG | 0.326527 | 4.1:+6271455 | MS.gene79469:CDS |
| TTCTTGTTGTTATCCTTCTT+GGG | 0.350090 | 4.1:-6270938 | None:intergenic |
| GGAAATGAAGGAGGAGGTGG+GGG | 0.366748 | 4.1:+6271458 | MS.gene79469:CDS |
| CAAGGATGTATTAATAAGAT+TGG | 0.369892 | 4.1:+6271156 | MS.gene79469:CDS |
| ATTTATCACAGAAGCTTAAG+AGG | 0.379478 | 4.1:+6271375 | MS.gene79469:CDS |
| AGGAAATGAAGGAGGAGGTG+GGG | 0.387875 | 4.1:+6271457 | MS.gene79469:CDS |
| AAGGAAATGAAGGAGGAGGT+GGG | 0.437708 | 4.1:+6271456 | MS.gene79469:CDS |
| GACAAGGAAGGTGGTGGTGG+TGG | 0.446982 | 4.1:+6271425 | MS.gene79469:CDS |
| TTGTTATCCTTCTTGGGAAG+TGG | 0.460354 | 4.1:-6270932 | None:intergenic |
| AAGGATGTATTAATAAGATT+GGG | 0.465298 | 4.1:+6271157 | MS.gene79469:CDS |
| CTATATGGAGGAGTAATGAT+AGG | 0.483553 | 4.1:+6270558 | None:intergenic |
| ACACACCCTTCAAGCAGAAA+AGG | 0.484551 | 4.1:-6271483 | None:intergenic |
| CATTTCACCACTTCCCAAGA+AGG | 0.504323 | 4.1:+6270925 | MS.gene79469:CDS |
| AAAGACAAGGAAGGTGGTGG+TGG | 0.508002 | 4.1:+6271422 | MS.gene79469:CDS |
| ATCACAGAAGCTTAAGAGGA+AGG | 0.524713 | 4.1:+6271379 | MS.gene79469:CDS |
| GGTGAGAAGGAAGGAAATGA+AGG | 0.524894 | 4.1:+6271446 | MS.gene79469:CDS |
| AAGGAAGGAAATGAAGGAGG+AGG | 0.525413 | 4.1:+6271452 | MS.gene79469:CDS |
| GGTGGTGGTGGTGAGAAGGA+AGG | 0.527573 | 4.1:+6271437 | MS.gene79469:CDS |
| GAAAGATAATGTGATTGTGA+AGG | 0.538961 | 4.1:+6271322 | MS.gene79469:CDS |
| AAAGATAATGTGATTGTGAA+GGG | 0.550811 | 4.1:+6271323 | MS.gene79469:CDS |
| GAAGCTTAAGAGGAAGGTTG+AGG | 0.551168 | 4.1:+6271385 | MS.gene79469:CDS |
| CAAGAAGGATAACAACAAGA+AGG | 0.577155 | 4.1:+6270940 | MS.gene79469:CDS |
| TGTGATTGTGAAGGGTACAA+TGG | 0.579067 | 4.1:+6271331 | MS.gene79469:CDS |
| AACAACAACATCAACAACAA+TGG | 0.582159 | 4.1:+6270818 | MS.gene79469:CDS |
| GAGAAGGAAGGAAATGAAGG+AGG | 0.583328 | 4.1:+6271449 | MS.gene79469:CDS |
| AAAATTGTTATGAAAACCAA+AGG | 0.601620 | 4.1:+6271180 | MS.gene79469:CDS |
| GAAGAAAACAACAACAGTGG+AGG | 0.608220 | 4.1:+6270854 | MS.gene79469:CDS |
| AAAGAAGAAAACAACAACAG+TGG | 0.609035 | 4.1:+6270851 | MS.gene79469:CDS |
| AAATTGGTACTACACTGTCA+AGG | 0.625937 | 4.1:+6271138 | MS.gene79469:CDS |
| AAGAAAGACAAGGAAGGTGG+TGG | 0.634788 | 4.1:+6271419 | MS.gene79469:CDS |
| AACAACATCAACAACAATGG+AGG | 0.637660 | 4.1:+6270821 | MS.gene79469:CDS |
| GGAGGAGATAAGAACAACAA+TGG | 0.639330 | 4.1:+6270872 | MS.gene79469:CDS |
| CCAAAGAAAGACAAGGAAGG+TGG | 0.648516 | 4.1:+6271416 | MS.gene79469:CDS |
| CAACCAAAGAAAGACAAGGA+AGG | 0.655515 | 4.1:+6271413 | MS.gene79469:CDS |
| AGTTCAACCAAAGAAAGACA+AGG | 0.673352 | 4.1:+6271409 | MS.gene79469:CDS |
| GGAGATAAGAACAACAATGG+AGG | 0.688227 | 4.1:+6270875 | MS.gene79469:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATTGTTATGAAAACCAA+AGG | + | chr4.1:6271180-6271199 | MS.gene79469:CDS | 20.0% |
| !! | AAGGATGTATTAATAAGATT+GGG | + | chr4.1:6271157-6271176 | MS.gene79469:CDS | 20.0% |
| !!! | ACTCTTTTTTTTTAATGACA+TGG | - | chr4.1:6270661-6270680 | None:intergenic | 20.0% |
| !!! | TTTTTTTAATGACATGGATT+TGG | - | chr4.1:6270655-6270674 | None:intergenic | 20.0% |
| ! | AAAGATAATGTGATTGTGAA+GGG | + | chr4.1:6271323-6271342 | MS.gene79469:CDS | 25.0% |
| ! | ATAGAAGAAGAATGAGAAAA+GGG | - | chr4.1:6270761-6270780 | None:intergenic | 25.0% |
| ! | ATTAATAAAAAGTTGCCAAC+AGG | - | chr4.1:6270621-6270640 | None:intergenic | 25.0% |
| ! | CAAGGATGTATTAATAAGAT+TGG | + | chr4.1:6271156-6271175 | MS.gene79469:CDS | 25.0% |
| ! | GAAAAATGAAGACAAAAAAG+AGG | + | chr4.1:6270994-6271013 | MS.gene79469:CDS | 25.0% |
| !!! | AATATGATTTTGATGTGTGT+AGG | + | chr4.1:6271272-6271291 | MS.gene79469:intron | 25.0% |
| !!! | TACTACTTCTGTTTTGAAAT+TGG | + | chr4.1:6271122-6271141 | MS.gene79469:CDS | 25.0% |
| !!! | TTCTCATCAGATTTTTTGTT+TGG | - | chr4.1:6270974-6270993 | None:intergenic | 25.0% |
| AAAGAAGAAAACAACAACAG+TGG | + | chr4.1:6270851-6270870 | MS.gene79469:CDS | 30.0% | |
| AACAACAACATCAACAACAA+TGG | + | chr4.1:6270818-6270837 | MS.gene79469:CDS | 30.0% | |
| ATTGTTATGAAAACCAAAGG+TGG | + | chr4.1:6271183-6271202 | MS.gene79469:intron | 30.0% | |
| ATTTATCACAGAAGCTTAAG+AGG | + | chr4.1:6271375-6271394 | MS.gene79469:CDS | 30.0% | |
| GAAAAATAAAAACCCACCTT+TGG | - | chr4.1:6271199-6271218 | None:intergenic | 30.0% | |
| GAAAGATAATGTGATTGTGA+AGG | + | chr4.1:6271322-6271341 | MS.gene79469:CDS | 30.0% | |
| GATAGAAGAAGAATGAGAAA+AGG | - | chr4.1:6270762-6270781 | None:intergenic | 30.0% | |
| TCAATCTTCATCCTTCATTA+TGG | + | chr4.1:6270786-6270805 | MS.gene79469:intron | 30.0% | |
| TTGTTATGAAAACCAAAGGT+GGG | + | chr4.1:6271184-6271203 | MS.gene79469:intron | 30.0% | |
| ! | TTTTCTTGAAGCCATAATGA+AGG | - | chr4.1:6270800-6270819 | None:intergenic | 30.0% |
| !! | TTCTTGTTGTTATCCTTCTT+GGG | - | chr4.1:6270941-6270960 | None:intergenic | 30.0% |
| AAACAACCGTAGCAAAGAAT+CGG | + | chr4.1:6270700-6270719 | MS.gene79469:intron | 35.0% | |
| AAATTGGTACTACACTGTCA+AGG | + | chr4.1:6271138-6271157 | MS.gene79469:CDS | 35.0% | |
| AACAACATCAACAACAATGG+AGG | + | chr4.1:6270821-6270840 | MS.gene79469:CDS | 35.0% | |
| AGAAAAGGGAGAAAAGGAAA+TGG | - | chr4.1:6270747-6270766 | None:intergenic | 35.0% | |
| AGAATGAGAAAAGGGAGAAA+AGG | - | chr4.1:6270753-6270772 | None:intergenic | 35.0% | |
| AGTTCAACCAAAGAAAGACA+AGG | + | chr4.1:6271409-6271428 | MS.gene79469:CDS | 35.0% | |
| ATAAAAAGTTGCCAACAGGA+AGG | - | chr4.1:6270617-6270636 | None:intergenic | 35.0% | |
| CAAGAAGGATAACAACAAGA+AGG | + | chr4.1:6270940-6270959 | MS.gene79469:CDS | 35.0% | |
| TAAAAAGTTGCCAACAGGAA+GGG | - | chr4.1:6270616-6270635 | None:intergenic | 35.0% | |
| !! | CTTCTTGTTGTTATCCTTCT+TGG | - | chr4.1:6270942-6270961 | None:intergenic | 35.0% |
| ATCACAGAAGCTTAAGAGGA+AGG | + | chr4.1:6271379-6271398 | MS.gene79469:CDS | 40.0% | |
| CAACCAAAGAAAGACAAGGA+AGG | + | chr4.1:6271413-6271432 | MS.gene79469:CDS | 40.0% | |
| GAAGAAAACAACAACAGTGG+AGG | + | chr4.1:6270854-6270873 | MS.gene79469:CDS | 40.0% | |
| GGAGATAAGAACAACAATGG+AGG | + | chr4.1:6270875-6270894 | MS.gene79469:CDS | 40.0% | |
| GGAGGAGATAAGAACAACAA+TGG | + | chr4.1:6270872-6270891 | MS.gene79469:CDS | 40.0% | |
| TTGTTATCCTTCTTGGGAAG+TGG | - | chr4.1:6270935-6270954 | None:intergenic | 40.0% | |
| ! | TGTGATTGTGAAGGGTACAA+TGG | + | chr4.1:6271331-6271350 | MS.gene79469:CDS | 40.0% |
| AAGAAAGACAAGGAAGGTGG+TGG | + | chr4.1:6271419-6271438 | MS.gene79469:CDS | 45.0% | |
| AAGGAAATGAAGGAGGAGGT+GGG | + | chr4.1:6271456-6271475 | MS.gene79469:CDS | 45.0% | |
| AAGGAAGGAAATGAAGGAGG+AGG | + | chr4.1:6271452-6271471 | MS.gene79469:CDS | 45.0% | |
| CATTTCACCACTTCCCAAGA+AGG | + | chr4.1:6270925-6270944 | MS.gene79469:CDS | 45.0% | |
| CCAAAGAAAGACAAGGAAGG+TGG | + | chr4.1:6271416-6271435 | MS.gene79469:CDS | 45.0% | |
| CCACCTTCCTTGTCTTTCTT+TGG | - | chr4.1:6271419-6271438 | None:intergenic | 45.0% | |
| GAAGCTTAAGAGGAAGGTTG+AGG | + | chr4.1:6271385-6271404 | MS.gene79469:CDS | 45.0% | |
| GAGAAGGAAGGAAATGAAGG+AGG | + | chr4.1:6271449-6271468 | MS.gene79469:CDS | 45.0% | |
| GATAGGTATACCACCATAGG+TGG | + | chr4.1:6270575-6270594 | MS.gene79469:intron | 45.0% | |
| GGTGAGAAGGAAGGAAATGA+AGG | + | chr4.1:6271446-6271465 | MS.gene79469:CDS | 45.0% | |
| TAGAGTGGTATTGCCACCTA+TGG | - | chr4.1:6270591-6270610 | None:intergenic | 45.0% | |
| ! | GATGAGCCGATTCTTTGCTA+CGG | - | chr4.1:6270709-6270728 | None:intergenic | 45.0% |
| !!! | CTTTTTATTAATAAAAAATT+TGG | + | chr4.1:6270629-6270648 | MS.gene79469:intron | 5.0% |
| !!! | TCATTTTTATTTTAATTTTT+TGG | + | chr4.1:6271063-6271082 | MS.gene79469:intron | 5.0% |
| AGGAAATGAAGGAGGAGGTG+GGG | + | chr4.1:6271457-6271476 | MS.gene79469:CDS | 50.0% | |
| AGTGGTATTGCCACCTATGG+TGG | - | chr4.1:6270588-6270607 | None:intergenic | 50.0% | |
| GAAGGAAATGAAGGAGGAGG+TGG | + | chr4.1:6271455-6271474 | MS.gene79469:CDS | 50.0% | |
| ! | AAAGACAAGGAAGGTGGTGG+TGG | + | chr4.1:6271422-6271441 | MS.gene79469:CDS | 50.0% |
| CCACTCTACACCCTTCCTGT+TGG | + | chr4.1:6270603-6270622 | MS.gene79469:intron | 55.0% | |
| GGAAATGAAGGAGGAGGTGG+GGG | + | chr4.1:6271458-6271477 | MS.gene79469:CDS | 55.0% | |
| ! | CCAACAGGAAGGGTGTAGAG+TGG | - | chr4.1:6270606-6270625 | None:intergenic | 55.0% |
| !! | AGGTGGTGGTGGTGGTGAGA+AGG | + | chr4.1:6271433-6271452 | MS.gene79469:CDS | 60.0% |
| !! | GACAAGGAAGGTGGTGGTGG+TGG | + | chr4.1:6271425-6271444 | MS.gene79469:CDS | 60.0% |
| !! | GGTGGTGGTGGTGAGAAGGA+AGG | + | chr4.1:6271437-6271456 | MS.gene79469:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 6270573 | 6271496 | 6270573 | ID=MS.gene79469 |
| chr4.1 | mRNA | 6270573 | 6271496 | 6270573 | ID=MS.gene79469.t1;Parent=MS.gene79469 |
| chr4.1 | exon | 6270573 | 6270579 | 6270573 | ID=MS.gene79469.t1.exon1;Parent=MS.gene79469.t1 |
| chr4.1 | CDS | 6270573 | 6270579 | 6270573 | ID=cds.MS.gene79469.t1;Parent=MS.gene79469.t1 |
| chr4.1 | exon | 6270816 | 6271015 | 6270816 | ID=MS.gene79469.t1.exon2;Parent=MS.gene79469.t1 |
| chr4.1 | CDS | 6270816 | 6271015 | 6270816 | ID=cds.MS.gene79469.t1;Parent=MS.gene79469.t1 |
| chr4.1 | exon | 6271117 | 6271201 | 6271117 | ID=MS.gene79469.t1.exon3;Parent=MS.gene79469.t1 |
| chr4.1 | CDS | 6271117 | 6271201 | 6271117 | ID=cds.MS.gene79469.t1;Parent=MS.gene79469.t1 |
| chr4.1 | exon | 6271294 | 6271496 | 6271294 | ID=MS.gene79469.t1.exon4;Parent=MS.gene79469.t1 |
| chr4.1 | CDS | 6271294 | 6271496 | 6271294 | ID=cds.MS.gene79469.t1;Parent=MS.gene79469.t1 |
| Gene Sequence |
| Protein sequence |
