Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80134.t1 | XP_013458297.1 | 51.6 | 95 | 46 | 0 | 36 | 130 | 71 | 165 | 4.70E-21 | 110.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80134.t1 | A0A072UTD4 | 51.6 | 95 | 46 | 0 | 36 | 130 | 71 | 165 | 3.4e-21 | 110.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060956 | MS.gene80134 | 0.801282 | 9.69E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80134.t1 | MTR_4g121040 | 51.579 | 95 | 46 | 0 | 36 | 130 | 71 | 165 | 3.97e-30 | 107 |
| MS.gene80134.t1 | MTR_8g042540 | 58.108 | 74 | 31 | 0 | 59 | 132 | 5 | 78 | 3.48e-26 | 94.7 |
| MS.gene80134.t1 | MTR_8g042540 | 58.108 | 74 | 31 | 0 | 59 | 132 | 17 | 90 | 5.67e-26 | 94.4 |
| MS.gene80134.t1 | MTR_4g032560 | 51.724 | 87 | 42 | 0 | 46 | 132 | 206 | 292 | 8.02e-26 | 99.0 |
| MS.gene80134.t1 | MTR_4g032580 | 51.724 | 87 | 42 | 0 | 46 | 132 | 206 | 292 | 8.02e-26 | 99.0 |
| MS.gene80134.t1 | MTR_7g110090 | 47.727 | 88 | 46 | 0 | 46 | 133 | 177 | 264 | 2.15e-24 | 94.7 |
| MS.gene80134.t1 | MTR_3g452180 | 46.591 | 88 | 47 | 0 | 46 | 133 | 93 | 180 | 3.61e-24 | 92.4 |
| MS.gene80134.t1 | MTR_1g105780 | 47.059 | 85 | 45 | 0 | 46 | 130 | 178 | 262 | 2.88e-21 | 86.7 |
| MS.gene80134.t1 | MTR_1g105765 | 47.059 | 85 | 45 | 0 | 46 | 130 | 178 | 262 | 3.10e-21 | 86.7 |
| MS.gene80134.t1 | MTR_1g069260 | 40.367 | 109 | 53 | 1 | 36 | 132 | 172 | 280 | 6.68e-21 | 85.9 |
| MS.gene80134.t1 | MTR_1g019580 | 46.667 | 90 | 42 | 1 | 47 | 130 | 30 | 119 | 9.25e-21 | 82.0 |
| MS.gene80134.t1 | MTR_0152s0060 | 46.341 | 82 | 44 | 0 | 46 | 127 | 228 | 309 | 3.28e-20 | 85.1 |
| MS.gene80134.t1 | MTR_1g047540 | 47.826 | 92 | 46 | 1 | 46 | 135 | 162 | 253 | 5.15e-20 | 85.1 |
| MS.gene80134.t1 | MTR_5g094740 | 48.193 | 83 | 43 | 0 | 48 | 130 | 727 | 809 | 5.75e-19 | 82.4 |
| MS.gene80134.t1 | MTR_1g087870 | 40.000 | 95 | 57 | 0 | 36 | 130 | 120 | 214 | 3.68e-18 | 78.2 |
| MS.gene80134.t1 | MTR_1g084880 | 40.860 | 93 | 55 | 0 | 46 | 138 | 182 | 274 | 5.36e-18 | 77.8 |
| MS.gene80134.t1 | MTR_4g062370 | 48.052 | 77 | 40 | 0 | 46 | 122 | 181 | 257 | 6.77e-18 | 79.3 |
| MS.gene80134.t1 | MTR_3g048970 | 39.785 | 93 | 56 | 0 | 46 | 138 | 182 | 274 | 6.48e-17 | 75.1 |
| MS.gene80134.t1 | MTR_4g081330 | 52.727 | 55 | 26 | 0 | 60 | 114 | 1085 | 1139 | 6.37e-14 | 67.8 |
| MS.gene80134.t1 | MTR_3g070250 | 39.535 | 86 | 52 | 0 | 46 | 131 | 187 | 272 | 1.50e-13 | 65.9 |
| MS.gene80134.t1 | MTR_4g057880 | 46.774 | 62 | 33 | 0 | 70 | 131 | 2 | 63 | 2.01e-13 | 61.6 |
| MS.gene80134.t1 | MTR_2g100360 | 39.759 | 83 | 50 | 0 | 36 | 118 | 172 | 254 | 1.98e-12 | 62.8 |
| MS.gene80134.t1 | MTR_2g016390 | 39.726 | 73 | 44 | 0 | 46 | 118 | 615 | 687 | 4.06e-12 | 62.8 |
| MS.gene80134.t1 | MTR_5g066660 | 39.759 | 83 | 50 | 0 | 50 | 132 | 154 | 236 | 5.57e-11 | 58.9 |
| MS.gene80134.t1 | MTR_1g037600 | 37.838 | 74 | 46 | 0 | 46 | 119 | 82 | 155 | 8.81e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATATGCTCATTTCAAAAT+TGG | 0.202477 | 5.1:+42310353 | MS.gene80134:CDS |
| CAAAACCTTTGTTCCACTTA+TGG | 0.230090 | 5.1:-42309900 | None:intergenic |
| GTCAATCAACTTCAAAGATT+TGG | 0.290974 | 5.1:-42310285 | None:intergenic |
| CTGAAAAGATTGAATCTGAT+TGG | 0.345346 | 5.1:+42310112 | MS.gene80134:CDS |
| GACTTAACTATGACAGAATT+CGG | 0.350525 | 5.1:+42309854 | None:intergenic |
| AGTCTCGTTTCTCCAGCTTT+CGG | 0.382166 | 5.1:-42310443 | None:intergenic |
| GTGGTTGGTGCACCGAAAGC+TGG | 0.465831 | 5.1:+42310431 | MS.gene80134:CDS |
| TTTATGAGATGACGTTGGAT+AGG | 0.487127 | 5.1:+42310258 | MS.gene80134:CDS |
| ATTTCAAAATTGGCGGTGGT+TGG | 0.496032 | 5.1:+42310363 | MS.gene80134:CDS |
| TTGACGACTGTGGTAACATA+TGG | 0.502440 | 5.1:+42310303 | MS.gene80134:CDS |
| TAATTCCAACGGTGGTATGA+AGG | 0.518797 | 5.1:+42309970 | MS.gene80134:CDS |
| ATGAGATGACGTTGGATAGG+AGG | 0.520878 | 5.1:+42310261 | MS.gene80134:CDS |
| TTCCAGGAACTTGCTTACAT+AGG | 0.533111 | 5.1:+42310233 | MS.gene80134:intron |
| TATGCTCATTTCAAAATTGG+CGG | 0.535363 | 5.1:+42310356 | MS.gene80134:CDS |
| GGTGGTGACAGTGCCATAAG+TGG | 0.543123 | 5.1:+42309887 | MS.gene80134:CDS |
| AAAGATTGAATCTGATTGGT+TGG | 0.556723 | 5.1:+42310116 | MS.gene80134:CDS |
| TGATGAGGCCGACGGTGGAG+TGG | 0.573797 | 5.1:+42309934 | MS.gene80134:CDS |
| GCTCATTTCAAAATTGGCGG+TGG | 0.581764 | 5.1:+42310359 | MS.gene80134:CDS |
| CATGACCTTCATACCACCGT+TGG | 0.590970 | 5.1:-42309975 | None:intergenic |
| CAACGGTGGTATGAAGGTCA+TGG | 0.593997 | 5.1:+42309976 | MS.gene80134:CDS |
| TCATCAAGAATTGTGTATCG+TGG | 0.596337 | 5.1:+42310412 | MS.gene80134:CDS |
| GGCTTTGATGAGGCCGACGG+TGG | 0.599577 | 5.1:+42309929 | MS.gene80134:CDS |
| TTTGAATGGTGGCTTTGATG+AGG | 0.613141 | 5.1:+42309919 | MS.gene80134:CDS |
| CAGTGCCATAAGTGGAACAA+AGG | 0.613432 | 5.1:+42309895 | MS.gene80134:CDS |
| CAAGAATTGTGTATCGTGGT+TGG | 0.630355 | 5.1:+42310416 | MS.gene80134:CDS |
| GAATCTCGCCACTCCACCGT+CGG | 0.638169 | 5.1:-42309942 | None:intergenic |
| ACAGAATTCGGTGGTTACAG+AGG | 0.640503 | 5.1:+42309866 | MS.gene80134:CDS |
| GAATTCGGTGGTTACAGAGG+TGG | 0.664609 | 5.1:+42309869 | MS.gene80134:CDS |
| GGTGGCTTTGATGAGGCCGA+CGG | 0.666667 | 5.1:+42309926 | MS.gene80134:CDS |
| AGATTCGTCGATAATTCCAA+CGG | 0.692357 | 5.1:+42309959 | MS.gene80134:CDS |
| TTCGTCGATAATTCCAACGG+TGG | 0.697195 | 5.1:+42309962 | MS.gene80134:CDS |
| AGCGAATGACACTAGCACGA+AGG | 0.704631 | 5.1:+42310387 | MS.gene80134:CDS |
| TTGAAGTTGATTGACGACTG+TGG | 0.709138 | 5.1:+42310293 | MS.gene80134:CDS |
| TTAACTATGACAGAATTCGG+TGG | 0.709225 | 5.1:+42309857 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAATTTCACAAATAGAAA+TGG | - | chr5.1:42310037-42310056 | None:intergenic | 15.0% |
| !! | ATAATTTCACAAATAGAAAT+GGG | - | chr5.1:42310036-42310055 | None:intergenic | 15.0% |
| !!! | GACTATTTTTGATGTTTATA+TGG | + | chr5.1:42310180-42310199 | MS.gene80134:intron | 20.0% |
| ! | CAATATGCTCATTTCAAAAT+TGG | + | chr5.1:42310353-42310372 | MS.gene80134:CDS | 25.0% |
| !! | TAACAAGTTAGTTTATTTCC+AGG | + | chr5.1:42310217-42310236 | MS.gene80134:intron | 25.0% |
| !!! | CAATTTTGAAATGAGCATAT+TGG | - | chr5.1:42310355-42310374 | None:intergenic | 25.0% |
| AAAGATTGAATCTGATTGGT+TGG | + | chr5.1:42310116-42310135 | MS.gene80134:CDS | 30.0% | |
| CTGAAAAGATTGAATCTGAT+TGG | + | chr5.1:42310112-42310131 | MS.gene80134:CDS | 30.0% | |
| GTCAATCAACTTCAAAGATT+TGG | - | chr5.1:42310288-42310307 | None:intergenic | 30.0% | |
| TATGCTCATTTCAAAATTGG+CGG | + | chr5.1:42310356-42310375 | MS.gene80134:CDS | 30.0% | |
| !!! | GAGCTTTGAATTTTTCTACA+AGG | + | chr5.1:42310077-42310096 | MS.gene80134:intron | 30.0% |
| AGATTCGTCGATAATTCCAA+CGG | + | chr5.1:42309959-42309978 | MS.gene80134:CDS | 35.0% | |
| CAAAACCTTTGTTCCACTTA+TGG | - | chr5.1:42309903-42309922 | None:intergenic | 35.0% | |
| TCATCAAGAATTGTGTATCG+TGG | + | chr5.1:42310412-42310431 | MS.gene80134:CDS | 35.0% | |
| TTTATGAGATGACGTTGGAT+AGG | + | chr5.1:42310258-42310277 | MS.gene80134:CDS | 35.0% | |
| ! | AGGTTTTTATGAGATGACGT+TGG | + | chr5.1:42310253-42310272 | MS.gene80134:CDS | 35.0% |
| !!! | AGTGGAACAAAGGTTTTGAA+TGG | + | chr5.1:42309905-42309924 | MS.gene80134:CDS | 35.0% |
| AACCTATGTAAGCAAGTTCC+TGG | - | chr5.1:42310238-42310257 | None:intergenic | 40.0% | |
| ATTTCAAAATTGGCGGTGGT+TGG | + | chr5.1:42310363-42310382 | MS.gene80134:CDS | 40.0% | |
| CAAGAATTGTGTATCGTGGT+TGG | + | chr5.1:42310416-42310435 | MS.gene80134:CDS | 40.0% | |
| TAATTCCAACGGTGGTATGA+AGG | + | chr5.1:42309970-42309989 | MS.gene80134:CDS | 40.0% | |
| TTGACGACTGTGGTAACATA+TGG | + | chr5.1:42310303-42310322 | MS.gene80134:CDS | 40.0% | |
| ! | TTCCAGGAACTTGCTTACAT+AGG | + | chr5.1:42310233-42310252 | MS.gene80134:intron | 40.0% |
| ! | TTGAAGTTGATTGACGACTG+TGG | + | chr5.1:42310293-42310312 | MS.gene80134:CDS | 40.0% |
| !! | TTTGAATGGTGGCTTTGATG+AGG | + | chr5.1:42309919-42309938 | MS.gene80134:CDS | 40.0% |
| !!! | GGAACAAAGGTTTTGAATGG+TGG | + | chr5.1:42309908-42309927 | MS.gene80134:CDS | 40.0% |
| AGGAACTCAGCAACTGTTGA+TGG | + | chr5.1:42310010-42310029 | MS.gene80134:intron | 45.0% | |
| AGGTCATGGTGTGACTAAAC+AGG | + | chr5.1:42309990-42310009 | MS.gene80134:intron | 45.0% | |
| AGTCTCGTTTCTCCAGCTTT+CGG | - | chr5.1:42310446-42310465 | None:intergenic | 45.0% | |
| ATGAGATGACGTTGGATAGG+AGG | + | chr5.1:42310261-42310280 | MS.gene80134:CDS | 45.0% | |
| CAGTGCCATAAGTGGAACAA+AGG | + | chr5.1:42309895-42309914 | MS.gene80134:CDS | 45.0% | |
| GCTCATTTCAAAATTGGCGG+TGG | + | chr5.1:42310359-42310378 | MS.gene80134:CDS | 45.0% | |
| TTCGTCGATAATTCCAACGG+TGG | + | chr5.1:42309962-42309981 | MS.gene80134:CDS | 45.0% | |
| ! | ACAGAATTCGGTGGTTACAG+AGG | + | chr5.1:42309866-42309885 | MS.gene80134:CDS | 45.0% |
| AGCGAATGACACTAGCACGA+AGG | + | chr5.1:42310387-42310406 | MS.gene80134:CDS | 50.0% | |
| CAACGGTGGTATGAAGGTCA+TGG | + | chr5.1:42309976-42309995 | MS.gene80134:CDS | 50.0% | |
| CATGACCTTCATACCACCGT+TGG | - | chr5.1:42309978-42309997 | None:intergenic | 50.0% | |
| ! | GAATTCGGTGGTTACAGAGG+TGG | + | chr5.1:42309869-42309888 | MS.gene80134:CDS | 50.0% |
| GGTGGTGACAGTGCCATAAG+TGG | + | chr5.1:42309887-42309906 | MS.gene80134:CDS | 55.0% | |
| GAATCTCGCCACTCCACCGT+CGG | - | chr5.1:42309945-42309964 | None:intergenic | 60.0% | |
| ! | GTGGTTGGTGCACCGAAAGC+TGG | + | chr5.1:42310431-42310450 | MS.gene80134:CDS | 60.0% |
| !! | GGTGGCTTTGATGAGGCCGA+CGG | + | chr5.1:42309926-42309945 | MS.gene80134:CDS | 60.0% |
| TGATGAGGCCGACGGTGGAG+TGG | + | chr5.1:42309934-42309953 | MS.gene80134:CDS | 65.0% | |
| ! | GGCTTTGATGAGGCCGACGG+TGG | + | chr5.1:42309929-42309948 | MS.gene80134:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 42309863 | 42310481 | 42309863 | ID=MS.gene80134 |
| chr5.1 | mRNA | 42309863 | 42310481 | 42309863 | ID=MS.gene80134.t1;Parent=MS.gene80134 |
| chr5.1 | exon | 42309863 | 42309997 | 42309863 | ID=MS.gene80134.t1.exon1;Parent=MS.gene80134.t1 |
| chr5.1 | CDS | 42309863 | 42309997 | 42309863 | ID=cds.MS.gene80134.t1;Parent=MS.gene80134.t1 |
| chr5.1 | exon | 42310099 | 42310137 | 42310099 | ID=MS.gene80134.t1.exon2;Parent=MS.gene80134.t1 |
| chr5.1 | CDS | 42310099 | 42310137 | 42310099 | ID=cds.MS.gene80134.t1;Parent=MS.gene80134.t1 |
| chr5.1 | exon | 42310239 | 42310481 | 42310239 | ID=MS.gene80134.t1.exon3;Parent=MS.gene80134.t1 |
| chr5.1 | CDS | 42310239 | 42310481 | 42310239 | ID=cds.MS.gene80134.t1;Parent=MS.gene80134.t1 |
| Gene Sequence |
| Protein sequence |
