AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80356

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80356.t1 MTR_6g035370 88.174 668 51 8 1 662 1 646 0.0 815
MS.gene80356.t1 MTR_1g098900 55.051 683 230 18 1 658 1 631 0.0 595
MS.gene80356.t1 MTR_1g098870 41.791 603 238 14 2 600 3 496 2.33e-125 383
MS.gene80356.t1 MTR_1g492790 52.756 127 55 2 54 175 113 239 9.66e-35 139
MS.gene80356.t1 MTR_1g492790 45.652 92 48 2 476 566 110 200 3.86e-19 91.7
MS.gene80356.t1 MTR_2g026725 31.597 288 145 7 281 568 115 350 1.53e-34 135
MS.gene80356.t1 MTR_2g026725 39.796 98 56 2 54 149 261 357 2.05e-16 82.0
MS.gene80356.t1 MTR_1g060990 64.583 96 31 2 46 140 124 217 5.61e-32 130
MS.gene80356.t1 MTR_1g060990 46.237 93 48 2 476 567 127 218 3.74e-19 91.7
MS.gene80356.t1 MTR_1g060990 37.383 107 67 0 251 357 264 370 3.64e-14 75.9
MS.gene80356.t1 MTR_7g114860 57.000 100 34 2 480 570 381 480 2.37e-28 119
MS.gene80356.t1 MTR_7g114860 41.837 98 47 2 55 142 381 478 7.98e-16 81.3
MS.gene80356.t1 MTR_1g081180 59.783 92 35 1 480 569 418 509 1.71e-26 114
MS.gene80356.t1 MTR_1g081180 39.785 93 53 2 53 142 416 508 6.48e-14 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80356.t1 AT1G76880 56.173 324 96 6 43 359 49 333 8.22e-93 300
MS.gene80356.t1 AT1G76880 65.584 154 38 3 458 598 382 533 4.39e-60 212
MS.gene80356.t1 AT1G76880 46.739 92 48 1 50 140 403 494 4.01e-19 92.0
MS.gene80356.t1 AT1G76880 47.253 91 47 1 479 569 60 149 6.32e-19 91.3
MS.gene80356.t1 AT1G76890 48.750 320 131 7 52 357 38 338 1.11e-81 269
MS.gene80356.t1 AT1G76890 64.029 139 40 3 459 597 378 506 4.52e-49 181
MS.gene80356.t1 AT1G76890 44.565 92 50 1 52 142 394 485 1.98e-19 92.8
MS.gene80356.t1 AT1G76890 43.182 88 49 1 479 566 40 126 5.14e-17 85.1
MS.gene80356.t1 AT5G28300 36.789 299 139 9 281 569 300 558 2.79e-44 168
MS.gene80356.t1 AT5G28300 39.815 108 54 3 48 144 452 559 2.58e-15 80.1
MS.gene80356.t1 AT5G03680 34.202 307 142 7 55 356 119 370 7.64e-44 166
MS.gene80356.t1 AT5G03680 44.211 95 51 2 480 573 119 212 4.67e-19 91.7
MS.gene80356.t1 AT1G33240 59.649 114 35 1 54 167 61 163 1.95e-38 150
MS.gene80356.t1 AT1G33240 59.649 114 35 1 54 167 61 163 3.97e-38 150
MS.gene80356.t1 AT3G10000 57.143 105 43 2 38 140 71 175 1.62e-30 125
MS.gene80356.t1 AT3G10000 44.086 93 51 1 476 567 84 176 1.46e-20 95.9
MS.gene80356.t1 AT3G10000 53.846 104 38 2 38 140 71 165 9.12e-27 114
MS.gene80356.t1 AT3G10000 41.304 92 45 1 476 567 84 166 2.06e-17 85.9
MS.gene80356.t1 AT5G47660 51.685 89 41 1 480 568 303 389 7.88e-22 99.0
MS.gene80356.t1 AT5G47660 47.059 85 40 3 55 137 303 384 4.49e-14 75.1
MS.gene80356.t1 AT1G33240 54.430 79 25 1 89 167 57 124 1.29e-18 90.5
MS.gene80356.t1 AT1G33240 37.815 119 72 2 55 171 396 514 2.12e-18 89.7

Find 193 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAGATGCAAGTGTTAA+AGG 0.186987 6.3:+27657998 MS.gene80356:CDS
TTCAGGTTTGATGAAGAAAA+TGG 0.194543 6.3:+27660361 MS.gene80356:CDS
CCATTATGGGAGGAGATTTC+AGG 0.224552 6.3:+27660344 MS.gene80356:CDS
TTTACCACCTCTTCCTTCTT+TGG 0.238671 6.3:-27659175 None:intergenic
ATGGACCTAAAGGGCCATTA+TGG 0.251711 6.3:+27660330 MS.gene80356:CDS
TCGGTGTCGGTGTTGGTGTT+GGG 0.265380 6.3:-27659980 None:intergenic
GATATGAAGTACCAAGAAAA+TGG 0.297874 6.3:+27660311 MS.gene80356:CDS
GGACATGTCTTTGAATCTTC+TGG 0.297895 6.3:-27660473 None:intergenic
TCCTCCCATAATGGCCCTTT+AGG 0.309914 6.3:-27660335 None:intergenic
GTTGTTGTTGATGATGGTAT+TGG 0.315589 6.3:-27659462 None:intergenic
AACAGGCGCCACCAGCAGCC+AGG 0.316128 6.3:+27660627 MS.gene80356:CDS
GGTGGTGGTGGATCAATTTC+AGG 0.335799 6.3:+27657848 MS.gene80356:CDS
GGAGTTGCACAAGAGGTTCT+TGG 0.339414 6.3:+27659710 MS.gene80356:CDS
GTCGGTGTCGGTGTTGGTGT+TGG 0.352301 6.3:-27659981 None:intergenic
GTCACATCCGCCCTGGCTGC+TGG 0.353901 6.3:-27660638 None:intergenic
TACTTTGGCTAATAGGAAAA+TGG 0.355696 6.3:+27659077 MS.gene80356:intron
GTTGAAGATAAGGTCTGATA+TGG 0.365393 6.3:+27657967 MS.gene80356:CDS
AGAGGGTTATTAGTATTTGT+AGG 0.377717 6.3:-27659492 None:intergenic
TACTAAAGATGCTGCTGTTA+TGG 0.378392 6.3:+27659851 MS.gene80356:CDS
AAAGCAACTGTTTCTTGTCT+TGG 0.384139 6.3:-27657944 None:intergenic
TCAGGTTTGATGAAGAAAAT+GGG 0.384923 6.3:+27660362 MS.gene80356:CDS
AATAGGAAAATGGCAGATCT+TGG 0.385060 6.3:+27659087 MS.gene80356:intron
CAAGTGTTAAAGGTCCCTTA+TGG 0.386222 6.3:+27658008 MS.gene80356:CDS
TTGAGGTGGTGGTGGTTTGT+TGG 0.387596 6.3:-27660786 None:intergenic
ACTGGCAATGGTGCTGCTGC+TGG 0.390612 6.3:-27660005 None:intergenic
GCAATGGCCTCCACAACAAC+AGG 0.393419 6.3:+27660610 MS.gene80356:CDS
TGAAGAAAATGGGGTACAAT+AGG 0.394111 6.3:+27660372 MS.gene80356:CDS
ACAGCAGCAAGTGACGAATA+TGG 0.394354 6.3:+27660184 MS.gene80356:CDS
TGCAAAGGATTAATAGAGAA+AGG 0.396339 6.3:+27659796 MS.gene80356:CDS
CAAAGAGGTGCAAAGAGAAA+TGG 0.396790 6.3:+27660399 MS.gene80356:CDS
AGAAATGAAGCTGTAGTTGT+AGG 0.397339 6.3:-27659333 None:intergenic
CCTGAAATCTCCTCCCATAA+TGG 0.399545 6.3:-27660344 None:intergenic
AACGATGATGGCACCGTTGA+TGG 0.403172 6.3:+27660574 MS.gene80356:CDS
GTCGGTGTTGGTGTTGGGGT+TGG 0.406448 6.3:-27659975 None:intergenic
CAGTTGCTTTGTTGAAGATA+AGG 0.412788 6.3:+27657957 MS.gene80356:CDS
GACTCAACAGTGTTGGGAAC+AGG 0.413588 6.3:+27657785 MS.gene80356:CDS
GAAATGAAGCTGTAGTTGTA+GGG 0.418405 6.3:-27659332 None:intergenic
CCTTGAAACTTCATCCCATA+AGG 0.419165 6.3:-27658023 None:intergenic
GGAACAGGAGGAGGCAGCTC+AGG 0.420665 6.3:+27657800 MS.gene80356:CDS
GAGGGTGTGGATAGAAGTTT+TGG 0.422223 6.3:+27657908 MS.gene80356:CDS
AAGTTCGTCTTCGACTTCTT+CGG 0.423944 6.3:+27659584 MS.gene80356:CDS
GTAATAGTAATTTGAGGAAG+AGG 0.435660 6.3:-27659510 None:intergenic
TGTGTGTAAGACATTGGAAT+TGG 0.436193 6.3:-27659417 None:intergenic
ACCAACTTGATGCTCTATAC+AGG 0.438141 6.3:+27660504 MS.gene80356:CDS
AGGGGTGGGAATAGTACTAA+TGG 0.438142 6.3:-27659313 None:intergenic
TGGACCTAAAGGGCCATTAT+GGG 0.446325 6.3:+27660331 MS.gene80356:CDS
TGTGTTTGTGTTTGAGTTGG+TGG 0.448747 6.3:-27659285 None:intergenic
GGTATTGCTGGTATTGGTAT+TGG 0.450653 6.3:-27660044 None:intergenic
ACGGTGTTGTTGTTCAATAC+AGG 0.451355 6.3:-27659915 None:intergenic
AAGTGTTAAAGGTCCCTTAT+GGG 0.453094 6.3:+27658009 MS.gene80356:CDS
GACGGAAGAGGAAGAGGAAA+TGG 0.453743 6.3:+27659640 MS.gene80356:CDS
GTTTGTGTTTGTGTTTGAGT+TGG 0.456148 6.3:-27659288 None:intergenic
CGAATCGACGGAAGAGGAAG+AGG 0.460674 6.3:+27659634 MS.gene80356:CDS
GGTGTAGGTATTGCTGGTAT+TGG 0.461156 6.3:-27660050 None:intergenic
AGCTGCTGCGTCGAGGCCTG+AGG 0.462044 6.3:+27660550 MS.gene80356:CDS
GTTGGTGGTGGTGTTATTGA+AGG 0.463101 6.3:-27659270 None:intergenic
AATGGTGCTGCTGCTGGTGT+CGG 0.467443 6.3:-27659999 None:intergenic
GCTGCTGCTGGTGTCGGTGT+CGG 0.467777 6.3:-27659993 None:intergenic
CCTTATGGGATGAAGTTTCA+AGG 0.471695 6.3:+27658023 MS.gene80356:CDS
GCATCAAGTTGGTGAAAGTA+AGG 0.472098 6.3:-27660494 None:intergenic
CTAGGGGACTCAACAGTGTT+GGG 0.473609 6.3:+27657779 MS.gene80356:CDS
TGTGGAGGCCATTGCTGCTC+CGG 0.474042 6.3:-27660602 None:intergenic
GGCAATGGTACTGCTGGTAC+TGG 0.477694 6.3:-27660023 None:intergenic
TGGAAGTAGTAGAAATGAAG+AGG 0.480126 6.3:+27657889 MS.gene80356:CDS
GCAAAGGATTAATAGAGAAA+GGG 0.481748 6.3:+27659797 MS.gene80356:CDS
TACCAAGAAAATGGACCTAA+AGG 0.481995 6.3:+27660320 MS.gene80356:CDS
GCTGGTGGCGCCTGTTGTTG+TGG 0.482373 6.3:-27660620 None:intergenic
TTTCTTTGAGAGACTAATGA+AGG 0.482390 6.3:+27659668 MS.gene80356:CDS
CTTGAAACTTCATCCCATAA+GGG 0.483873 6.3:-27658022 None:intergenic
TAGTAGAAATGAAGAGGGTG+TGG 0.484511 6.3:+27657895 MS.gene80356:CDS
GAGGGTTATTAGTATTTGTA+GGG 0.485687 6.3:-27659491 None:intergenic
TGGTGGAAAATGTGTAGGGT+TGG 0.486037 6.3:-27659442 None:intergenic
CGGTGTCGGTGTTGGTGTTG+GGG 0.486815 6.3:-27659979 None:intergenic
GGAAACGACAACAATGGAAG+GGG 0.487557 6.3:+27659608 MS.gene80356:CDS
CTCCATGGTCACATCCGCCC+TGG 0.491677 6.3:-27660645 None:intergenic
ATAGGAAAATGGCAGATCTT+GGG 0.492836 6.3:+27659088 MS.gene80356:intron
ACCAAGAAAATGGACCTAAA+GGG 0.493139 6.3:+27660321 MS.gene80356:CDS
ACATCAGCAGCAGCAACAAC+AGG 0.496241 6.3:+27660151 MS.gene80356:CDS
GCAGCAGCAACAACAGGTTC+AGG 0.497898 6.3:+27660157 MS.gene80356:CDS
GCTGGTGTCGGTGTCGGTGT+TGG 0.501369 6.3:-27659987 None:intergenic
GGCAGCTCAGGAGATGGAGC+AGG 0.501746 6.3:+27657812 MS.gene80356:CDS
GGGGATCGAATCGACGGAAG+AGG 0.501747 6.3:+27659628 MS.gene80356:CDS
GGAATTGGAAGTGTAGTTGA+TGG 0.503022 6.3:-27659402 None:intergenic
TGTTGCGGTGTAGGTATTGC+TGG 0.503047 6.3:-27660056 None:intergenic
GGCACCGTTGATGGTTCAGC+CGG 0.506059 6.3:+27660583 MS.gene80356:CDS
TGTTGCTGCTGCTGATGTTG+TGG 0.506243 6.3:-27660146 None:intergenic
GAAGGTGATTGATGCATTGA+AGG 0.506401 6.3:-27659252 None:intergenic
AAAGAGGTGCAAAGAGAAAT+GGG 0.507157 6.3:+27660400 MS.gene80356:CDS
GTTTGAACTTGTTGCGGTGT+AGG 0.516119 6.3:-27660065 None:intergenic
CACTCCTATGCAGCTATGCT+AGG 0.516756 6.3:+27657761 None:intergenic
AACAGCACCGGTCATTCCTC+AGG 0.519741 6.3:+27660112 MS.gene80356:CDS
GGTGCCATCATCGTTCCCTC+AGG 0.524674 6.3:-27660566 None:intergenic
ACTCCTATGCAGCTATGCTA+GGG 0.525198 6.3:+27657762 None:intergenic
GAGGTGCTAGAGAAGAAGCT+TGG 0.525337 6.3:+27659760 MS.gene80356:CDS
GAGAAAAGAGAAAGAGAGAG+AGG 0.527477 6.3:+27659741 MS.gene80356:CDS
TGGTTCAGCCGGAGCAGCAA+TGG 0.528420 6.3:+27660594 MS.gene80356:CDS
GCTAGGGGACTCAACAGTGT+TGG 0.529101 6.3:+27657778 MS.gene80356:CDS
TGATCGGCCGTGTCGAGATG+AGG 0.530163 6.3:+27660688 MS.gene80356:CDS
ACAGTGTTGGGAACAGGAGG+AGG 0.532710 6.3:+27657791 MS.gene80356:CDS
TGATGCATGCAAGTTCATCT+AGG 0.535689 6.3:+27660240 MS.gene80356:CDS
AACAAGAGGAGTTGCACAAG+AGG 0.535913 6.3:+27659703 MS.gene80356:CDS
GAAATATTAGCGAATGAAGA+TGG 0.536488 6.3:-27659540 None:intergenic
TGTTGTTGCACTGTTACAGT+TGG 0.536680 6.3:-27660089 None:intergenic
GCTGCTGCGTCGAGGCCTGA+GGG 0.536821 6.3:+27660551 MS.gene80356:CDS
GCTTACCATGATGGTGGTGG+TGG 0.537546 6.3:+27657836 MS.gene80356:CDS
ACATCCGCCCTGGCTGCTGG+TGG 0.538679 6.3:-27660635 None:intergenic
GGGGTGGGAATAGTACTAAT+GGG 0.539998 6.3:-27659312 None:intergenic
TGCTCCGGCTGAACCATCAA+CGG 0.540154 6.3:-27660587 None:intergenic
TAATAGTAATTTGAGGAAGA+GGG 0.542471 6.3:-27659509 None:intergenic
GGTATTGGTGGAAAATGTGT+AGG 0.543430 6.3:-27659447 None:intergenic
GGCGAAGCCTGAGGAATGAC+CGG 0.543584 6.3:-27660119 None:intergenic
GAGGCCTGAGGGAACGATGA+TGG 0.544953 6.3:+27660562 MS.gene80356:CDS
GGAGGAGGCAGCTCAGGAGA+TGG 0.546943 6.3:+27657806 MS.gene80356:CDS
GGAGGTGTAGAGTTTAACAA+TGG 0.547437 6.3:-27659366 None:intergenic
TGAAGGAAAGCAACAAGAAG+AGG 0.547925 6.3:+27660450 MS.gene80356:CDS
GTTTGTGTTTGAGTTGGTGG+TGG 0.548570 6.3:-27659282 None:intergenic
GGAAGAGGTGGTAAATCAGA+TGG 0.550653 6.3:+27659183 MS.gene80356:CDS
TTGGATTGTGTGTAAGACAT+TGG 0.551286 6.3:-27659423 None:intergenic
ACAGGCGCCACCAGCAGCCA+GGG 0.555235 6.3:+27660628 MS.gene80356:CDS
GTGCTAGAGAAGAAGCTTGG+AGG 0.555286 6.3:+27659763 MS.gene80356:CDS
TTGTTGTTGTTGAGGAATGA+CGG 0.557825 6.3:-27659934 None:intergenic
TATTAGCGAATGAAGATGGT+GGG 0.558390 6.3:-27659536 None:intergenic
GTTGTTGATGATGGTATTGG+TGG 0.559014 6.3:-27659459 None:intergenic
TGCTGCTGATGTTGTGGCAA+CGG 0.560332 6.3:-27660140 None:intergenic
TGAAGCTGTAGTTGTAGGGG+TGG 0.560356 6.3:-27659328 None:intergenic
GAGGAAACGACAACAATGGA+AGG 0.562272 6.3:+27659606 MS.gene80356:CDS
TGCGGCGCTTGTTGTTGTTG+AGG 0.562772 6.3:-27659942 None:intergenic
TCAACAACAACAAGCGCCGC+AGG 0.568754 6.3:+27659944 MS.gene80356:CDS
ATTAGCGAATGAAGATGGTG+GGG 0.569029 6.3:-27659535 None:intergenic
TATCACAAGAGAACCAAAGA+AGG 0.569305 6.3:+27659162 MS.gene80356:CDS
GGAGGTTGCAAGAGATGCAA+AGG 0.569352 6.3:+27659781 MS.gene80356:CDS
TTTCAGGTGATGATGAAAGA+GGG 0.571339 6.3:+27657864 MS.gene80356:CDS
TTGATCCACCACCACCATCA+TGG 0.573497 6.3:-27657841 None:intergenic
AAGAGAACCAAAGAAGGAAG+AGG 0.573797 6.3:+27659168 MS.gene80356:CDS
TTCATTCGCTAATATTTCAG+CGG 0.578885 6.3:+27659545 MS.gene80356:CDS
TTCGTCTTCGACTTCTTCGG+AGG 0.579514 6.3:+27659587 MS.gene80356:CDS
CAGGCAGAAGAACAAGGTGG+AGG 0.580136 6.3:+27660523 MS.gene80356:CDS
CAGGTTTGATGAAGAAAATG+GGG 0.580467 6.3:+27660363 MS.gene80356:CDS
GCTGGTATTGGTATTGGCAA+TGG 0.581527 6.3:-27660038 None:intergenic
GGTATTGGCAATGGTACTGC+TGG 0.582926 6.3:-27660029 None:intergenic
AAATGAAGCTGTAGTTGTAG+GGG 0.584207 6.3:-27659331 None:intergenic
GGTACTGCTGGTACTGGCAA+TGG 0.588980 6.3:-27660017 None:intergenic
GGAGCAGGAGCTTACCATGA+TGG 0.589395 6.3:+27657827 MS.gene80356:CDS
CTGAGAATGCTGAGAATGAT+CGG 0.591465 6.3:+27660672 MS.gene80356:CDS
ATTTCAGGTGATGATGAAAG+AGG 0.592121 6.3:+27657863 MS.gene80356:CDS
AGAACCAAAGAAGGAAGAGG+TGG 0.595193 6.3:+27659171 MS.gene80356:CDS
GATGACGAGGACGAAGATGA+AGG 0.597081 6.3:+27660740 MS.gene80356:CDS
AGCATTCTCAGCATCCTCCA+TGG 0.598685 6.3:-27660660 None:intergenic
GAATATTATGACGATGATGA+CGG 0.600596 6.3:+27660713 MS.gene80356:CDS
GGCGCCACCAGCAGCCAGGG+CGG 0.602641 6.3:+27660631 MS.gene80356:CDS
AGGGTGCAGCTGCTGCGTCG+AGG 0.603837 6.3:+27660543 MS.gene80356:CDS
GGAAGTAGTAGAAATGAAGA+GGG 0.606377 6.3:+27657890 MS.gene80356:CDS
TTCGGAGGAAACGACAACAA+TGG 0.607000 6.3:+27659602 MS.gene80356:CDS
GGAGGTTGTTGAGAAACAAG+AGG 0.607799 6.3:+27659689 MS.gene80356:CDS
CTTTGAGAGACTAATGAAGG+AGG 0.607887 6.3:+27659671 MS.gene80356:CDS
GAAGCTGTAGTTGTAGGGGT+GGG 0.608375 6.3:-27659327 None:intergenic
GCCTGTATAGAGCATCAAGT+TGG 0.608740 6.3:-27660505 None:intergenic
GAAGAGGAAGAGGAAATGGA+AGG 0.609651 6.3:+27659644 MS.gene80356:CDS
TTAGCGAATGAAGATGGTGG+GGG 0.612373 6.3:-27659534 None:intergenic
GTTGATGGAACAGTGGTGGT+AGG 0.616670 6.3:-27659387 None:intergenic
GTATTGGTGGAAAATGTGTA+GGG 0.617041 6.3:-27659446 None:intergenic
AAGGTGATTGATGCATTGAA+GGG 0.619014 6.3:-27659251 None:intergenic
TCAACAGTGTTGGGAACAGG+AGG 0.620361 6.3:+27657788 MS.gene80356:CDS
GATGGAACAGTGGTGGTAGG+AGG 0.624878 6.3:-27659384 None:intergenic
TCTATACAGGCAGAAGAACA+AGG 0.628687 6.3:+27660517 MS.gene80356:CDS
ATATTAGCGAATGAAGATGG+TGG 0.629833 6.3:-27659537 None:intergenic
ACCTAAAGGGCCATTATGGG+AGG 0.631216 6.3:+27660334 MS.gene80356:CDS
TGTGGCAACGGCGAAGCCTG+AGG 0.633518 6.3:-27660128 None:intergenic
TTCAGGTGATGATGAAAGAG+GGG 0.634874 6.3:+27657865 MS.gene80356:CDS
AGCCAGGGCGGATGTGACCA+TGG 0.636181 6.3:+27660643 MS.gene80356:CDS
GGTGTAGAGTTTAACAATGG+AGG 0.640467 6.3:-27659363 None:intergenic
CTCCTATGCAGCTATGCTAG+GGG 0.640615 6.3:+27657763 None:intergenic
AGGAAACGACAACAATGGAA+GGG 0.641866 6.3:+27659607 MS.gene80356:CDS
AGGCAGAAGAACAAGGTGGA+GGG 0.643787 6.3:+27660524 MS.gene80356:CDS
GTTGGAGTTTGAACTTGTTG+CGG 0.644658 6.3:-27660071 None:intergenic
GGAGCTTACCATGATGGTGG+TGG 0.649488 6.3:+27657833 MS.gene80356:CDS
GTGTTGTTGTTCAATACAGG+AGG 0.651178 6.3:-27659912 None:intergenic
AGTGCAACAACAAACAGCAC+CGG 0.652497 6.3:+27660100 MS.gene80356:CDS
GGGGTTGGTGTAGTTTCCTG+CGG 0.653086 6.3:-27659960 None:intergenic
GGTGGCGCCTGTTGTTGTGG+AGG 0.655534 6.3:-27660617 None:intergenic
TCAGGTGATGATGAAAGAGG+GGG 0.658562 6.3:+27657866 MS.gene80356:CDS
GAAACGACAACAATGGAAGG+GGG 0.686144 6.3:+27659609 MS.gene80356:CDS
TGCATGCAAGTTCATCTAGG+TGG 0.686396 6.3:+27660243 MS.gene80356:CDS
TATTCTTCCTCATCTCGACA+CGG 0.701910 6.3:-27660695 None:intergenic
GCAGGAGCTTACCATGATGG+TGG 0.707615 6.3:+27657830 MS.gene80356:CDS
GTTGCGAAAAGTGACAACAA+TGG 0.712598 6.3:+27660209 MS.gene80356:CDS
AAGTGTAGTTGATGGAACAG+TGG 0.713309 6.3:-27659394 None:intergenic
AGGTAGTGACAATTATGAAG+TGG 0.722373 6.3:+27660760 MS.gene80356:CDS
GGTACAATAGGAATGCAAAG+AGG 0.724275 6.3:+27660384 MS.gene80356:CDS
TGTAGTTGATGGAACAGTGG+TGG 0.725823 6.3:-27659391 None:intergenic
CAGGGCGGATGTGACCATGG+AGG 0.727693 6.3:+27660646 MS.gene80356:CDS
ATACAGGCAGAAGAACAAGG+TGG 0.746845 6.3:+27660520 MS.gene80356:CDS
TGATGACGGTGATGATGACG+AGG 0.768255 6.3:+27660727 MS.gene80356:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAAAAATAAAATTTC+TGG - chr6.3:27659031-27659050 None:intergenic 10.0%
!! TAAATTAAAAATATACATGA+AGG - chr6.3:27658445-27658464 None:intergenic 10.0%
!!! ATTAATTTTGTCTTTATTTT+TGG + chr6.3:27659053-27659072 MS.gene80356:intron 10.0%
!!! TATTTTTTTTATCTTTGATT+TGG + chr6.3:27658346-27658365 MS.gene80356:intron 10.0%
!!! TTAATTTTGTCTTTATTTTT+GGG + chr6.3:27659054-27659073 MS.gene80356:intron 10.0%
!! CATAAGATAATAAATGAATT+GGG - chr6.3:27658959-27658978 None:intergenic 15.0%
!! GTTGAAAAATAAAAATATGA+TGG + chr6.3:27658691-27658710 MS.gene80356:intron 15.0%
!!! AAATTTTTCTTTGCATTTTT+TGG - chr6.3:27659127-27659146 None:intergenic 15.0%
!!! TATTTTTCAACACTAAAAAT+GGG - chr6.3:27658682-27658701 None:intergenic 15.0%
!!! TTATTTTTCAACACTAAAAA+TGG - chr6.3:27658683-27658702 None:intergenic 15.0%
!! AACTTAAGTGATTAAGATAA+GGG - chr6.3:27658884-27658903 None:intergenic 20.0%
!! GCATAAGATAATAAATGAAT+TGG - chr6.3:27658960-27658979 None:intergenic 20.0%
!! TAACTTAAGTGATTAAGATA+AGG - chr6.3:27658885-27658904 None:intergenic 20.0%
!! TCTTATCTCATTAAATCTTT+AGG - chr6.3:27659003-27659022 None:intergenic 20.0%
!!! ATTAAAAAGTTTGTTTCTCA+TGG - chr6.3:27658759-27658778 None:intergenic 20.0%
!!! TATATCAAAGTTGATGTAAT+TGG - chr6.3:27658839-27658858 None:intergenic 20.0%
!!! TTCAAGTTTGTAACTTTATT+TGG - chr6.3:27658612-27658631 None:intergenic 20.0%
!!! TTTTTTTTTCAGATTTCAGA+AGG - chr6.3:27658134-27658153 None:intergenic 20.0%
! AAAAATGGGTAACTGAAATA+AGG - chr6.3:27658668-27658687 None:intergenic 25.0%
! AAAATGGGTAACTGAAATAA+GGG - chr6.3:27658667-27658686 None:intergenic 25.0%
! TAATAGTAATTTGAGGAAGA+GGG - chr6.3:27659512-27659531 None:intergenic 25.0%
! TAGGATAAGAAGCAATTAAT+TGG - chr6.3:27658984-27659003 None:intergenic 25.0%
! TCAATAATTCAGATTTCTCA+AGG - chr6.3:27658287-27658306 None:intergenic 25.0%
!!! CAAGTATTTTAAGAAAGTGA+AGG + chr6.3:27660433-27660452 MS.gene80356:CDS 25.0%
AGACAACCAAAGAATTGAAA+TGG - chr6.3:27658914-27658933 None:intergenic 30.0%
AGAGGGTTATTAGTATTTGT+AGG - chr6.3:27659495-27659514 None:intergenic 30.0%
ATAATTCAGATTTCTCAAGG+TGG - chr6.3:27658284-27658303 None:intergenic 30.0%
CAAAATCTTTGTTCACACTT+TGG + chr6.3:27658721-27658740 MS.gene80356:intron 30.0%
GAAATATTAGCGAATGAAGA+TGG - chr6.3:27659543-27659562 None:intergenic 30.0%
GAATATTATGACGATGATGA+CGG + chr6.3:27660713-27660732 MS.gene80356:CDS 30.0%
GAGGGTTATTAGTATTTGTA+GGG - chr6.3:27659494-27659513 None:intergenic 30.0%
GCAAAGGATTAATAGAGAAA+GGG + chr6.3:27659797-27659816 MS.gene80356:CDS 30.0%
GTAATAGTAATTTGAGGAAG+AGG - chr6.3:27659513-27659532 None:intergenic 30.0%
TACTTTGGCTAATAGGAAAA+TGG + chr6.3:27659077-27659096 MS.gene80356:intron 30.0%
TCAGGTTTGATGAAGAAAAT+GGG + chr6.3:27660362-27660381 MS.gene80356:CDS 30.0%
TGCAAAGGATTAATAGAGAA+AGG + chr6.3:27659796-27659815 MS.gene80356:CDS 30.0%
TTCAAAGATGCAAGTGTTAA+AGG + chr6.3:27657998-27658017 MS.gene80356:CDS 30.0%
TTCATTCGCTAATATTTCAG+CGG + chr6.3:27659545-27659564 MS.gene80356:CDS 30.0%
! GATATGAAGTACCAAGAAAA+TGG + chr6.3:27660311-27660330 MS.gene80356:CDS 30.0%
! TTCAGGTTTGATGAAGAAAA+TGG + chr6.3:27660361-27660380 MS.gene80356:CDS 30.0%
! TTTCTTTGAGAGACTAATGA+AGG + chr6.3:27659668-27659687 MS.gene80356:CDS 30.0%
!! GGAAGAAAATCTTTGCTTTT+TGG - chr6.3:27658643-27658662 None:intergenic 30.0%
!! TTTGTTGTTGTTGTTGATGA+TGG - chr6.3:27659471-27659490 None:intergenic 30.0%
!!! GTCTTTATTTTTGGGTACTT+TGG + chr6.3:27659062-27659081 MS.gene80356:intron 30.0%
!!! TTTTTTCAGATTTCAGAAGG+TGG - chr6.3:27658131-27658150 None:intergenic 30.0%
AAATGAAGCTGTAGTTGTAG+GGG - chr6.3:27659334-27659353 None:intergenic 35.0%
AAGGTGATTGATGCATTGAA+GGG - chr6.3:27659254-27659273 None:intergenic 35.0%
AAGTGATTAAGATAAGGGTG+AGG - chr6.3:27658879-27658898 None:intergenic 35.0%
AAGTGTTAAAGGTCCCTTAT+GGG + chr6.3:27658009-27658028 MS.gene80356:CDS 35.0%
AATAGGAAAATGGCAGATCT+TGG + chr6.3:27659087-27659106 MS.gene80356:intron 35.0%
ACCAAGAAAATGGACCTAAA+GGG + chr6.3:27660321-27660340 MS.gene80356:CDS 35.0%
AGAAATGAAGCTGTAGTTGT+AGG - chr6.3:27659336-27659355 None:intergenic 35.0%
AGGTAGTGACAATTATGAAG+TGG + chr6.3:27660760-27660779 MS.gene80356:CDS 35.0%
ATAGGAAAATGGCAGATCTT+GGG + chr6.3:27659088-27659107 MS.gene80356:intron 35.0%
ATATTAGCGAATGAAGATGG+TGG - chr6.3:27659540-27659559 None:intergenic 35.0%
CAGGTTTGATGAAGAAAATG+GGG + chr6.3:27660363-27660382 MS.gene80356:CDS 35.0%
CTTGAAACTTCATCCCATAA+GGG - chr6.3:27658025-27658044 None:intergenic 35.0%
CTTGCTCCATTTCAATTCTT+TGG + chr6.3:27658905-27658924 MS.gene80356:intron 35.0%
GAAATGAAGCTGTAGTTGTA+GGG - chr6.3:27659335-27659354 None:intergenic 35.0%
GGAAGTAGTAGAAATGAAGA+GGG + chr6.3:27657890-27657909 MS.gene80356:CDS 35.0%
GTTGAAGATAAGGTCTGATA+TGG + chr6.3:27657967-27657986 MS.gene80356:CDS 35.0%
GTTTGTGTTTGTGTTTGAGT+TGG - chr6.3:27659291-27659310 None:intergenic 35.0%
TACCAAGAAAATGGACCTAA+AGG + chr6.3:27660320-27660339 MS.gene80356:CDS 35.0%
TACTAAAGATGCTGCTGTTA+TGG + chr6.3:27659851-27659870 MS.gene80356:CDS 35.0%
TATCACAAGAGAACCAAAGA+AGG + chr6.3:27659162-27659181 MS.gene80356:CDS 35.0%
TATTAGCGAATGAAGATGGT+GGG - chr6.3:27659539-27659558 None:intergenic 35.0%
TGAAGAAAATGGGGTACAAT+AGG + chr6.3:27660372-27660391 MS.gene80356:CDS 35.0%
TGGAAGTAGTAGAAATGAAG+AGG + chr6.3:27657889-27657908 MS.gene80356:CDS 35.0%
TGTGTGTAAGACATTGGAAT+TGG - chr6.3:27659420-27659439 None:intergenic 35.0%
TTGGATTGTGTGTAAGACAT+TGG - chr6.3:27659426-27659445 None:intergenic 35.0%
! AAAGAGGTGCAAAGAGAAAT+GGG + chr6.3:27660400-27660419 MS.gene80356:CDS 35.0%
! AAAGCAACTGTTTCTTGTCT+TGG - chr6.3:27657947-27657966 None:intergenic 35.0%
! ATTTCAGGTGATGATGAAAG+AGG + chr6.3:27657863-27657882 MS.gene80356:CDS 35.0%
! CAGTTGCTTTGTTGAAGATA+AGG + chr6.3:27657957-27657976 MS.gene80356:CDS 35.0%
! GTATTGGTGGAAAATGTGTA+GGG - chr6.3:27659449-27659468 None:intergenic 35.0%
! TTGTTGTTGTTGAGGAATGA+CGG - chr6.3:27659937-27659956 None:intergenic 35.0%
! TTTCAGGTGATGATGAAAGA+GGG + chr6.3:27657864-27657883 MS.gene80356:CDS 35.0%
!! CATTGAAGGGTTGTTTTCAA+GGG - chr6.3:27659241-27659260 None:intergenic 35.0%
!! GAAGTTTTGGTGGTAATAGA+TGG + chr6.3:27657921-27657940 MS.gene80356:CDS 35.0%
!! GTTGTTGTTGATGATGGTAT+TGG - chr6.3:27659465-27659484 None:intergenic 35.0%
!! TTTTGGGTACTTTGGCTAAT+AGG + chr6.3:27659070-27659089 MS.gene80356:intron 35.0%
AAGAGAACCAAAGAAGGAAG+AGG + chr6.3:27659168-27659187 MS.gene80356:CDS 40.0%
AAGTTCGTCTTCGACTTCTT+CGG + chr6.3:27659584-27659603 MS.gene80356:CDS 40.0%
ACCAACTTGATGCTCTATAC+AGG + chr6.3:27660504-27660523 MS.gene80356:CDS 40.0%
AGGAAACGACAACAATGGAA+GGG + chr6.3:27659607-27659626 MS.gene80356:CDS 40.0%
ATGGGTAACTGAAATAAGGG+AGG - chr6.3:27658664-27658683 None:intergenic 40.0%
ATTAGCGAATGAAGATGGTG+GGG - chr6.3:27659538-27659557 None:intergenic 40.0%
CAAGTGTTAAAGGTCCCTTA+TGG + chr6.3:27658008-27658027 MS.gene80356:CDS 40.0%
CCTTATGGGATGAAGTTTCA+AGG + chr6.3:27658023-27658042 MS.gene80356:CDS 40.0%
CCTTGAAACTTCATCCCATA+AGG - chr6.3:27658026-27658045 None:intergenic 40.0%
CTGAGAATGCTGAGAATGAT+CGG + chr6.3:27660672-27660691 MS.gene80356:CDS 40.0%
GAAGGTGATTGATGCATTGA+AGG - chr6.3:27659255-27659274 None:intergenic 40.0%
GAGAAAAGAGAAAGAGAGAG+AGG + chr6.3:27659741-27659760 MS.gene80356:CDS 40.0%
GGACATGTCTTTGAATCTTC+TGG - chr6.3:27660476-27660495 None:intergenic 40.0%
GGTACAATAGGAATGCAAAG+AGG + chr6.3:27660384-27660403 MS.gene80356:CDS 40.0%
GGTGTAGAGTTTAACAATGG+AGG - chr6.3:27659366-27659385 None:intergenic 40.0%
GTTGCGAAAAGTGACAACAA+TGG + chr6.3:27660209-27660228 MS.gene80356:CDS 40.0%
TAGTAGAAATGAAGAGGGTG+TGG + chr6.3:27657895-27657914 MS.gene80356:CDS 40.0%
TATTCTTCCTCATCTCGACA+CGG - chr6.3:27660698-27660717 None:intergenic 40.0%
TCTATACAGGCAGAAGAACA+AGG + chr6.3:27660517-27660536 MS.gene80356:CDS 40.0%
TGAAGGAAAGCAACAAGAAG+AGG + chr6.3:27660450-27660469 MS.gene80356:CDS 40.0%
TGATGCATGCAAGTTCATCT+AGG + chr6.3:27660240-27660259 MS.gene80356:CDS 40.0%
TGTTGTTGCACTGTTACAGT+TGG - chr6.3:27660092-27660111 None:intergenic 40.0%
TTTACCACCTCTTCCTTCTT+TGG - chr6.3:27659178-27659197 None:intergenic 40.0%
! AATGCCTCAACCTCAGTTTT+TGG - chr6.3:27660269-27660288 None:intergenic 40.0%
! CAAAGAGGTGCAAAGAGAAA+TGG + chr6.3:27660399-27660418 MS.gene80356:CDS 40.0%
! CTTTGAGAGACTAATGAAGG+AGG + chr6.3:27659671-27659690 MS.gene80356:CDS 40.0%
! GGAATTGGAAGTGTAGTTGA+TGG - chr6.3:27659405-27659424 None:intergenic 40.0%
! GGAGGTGTAGAGTTTAACAA+TGG - chr6.3:27659369-27659388 None:intergenic 40.0%
! GGGGGTGTAATAGTAATTTG+AGG - chr6.3:27659519-27659538 None:intergenic 40.0%
! GTGTTGTTGTTCAATACAGG+AGG - chr6.3:27659915-27659934 None:intergenic 40.0%
! TTCAGGTGATGATGAAAGAG+GGG + chr6.3:27657865-27657884 MS.gene80356:CDS 40.0%
!! AAGTGTAGTTGATGGAACAG+TGG - chr6.3:27659397-27659416 None:intergenic 40.0%
!! ACGGTGTTGTTGTTCAATAC+AGG - chr6.3:27659918-27659937 None:intergenic 40.0%
!! GCATCAAGTTGGTGAAAGTA+AGG - chr6.3:27660497-27660516 None:intergenic 40.0%
!! GCATTGAAGGGTTGTTTTCA+AGG - chr6.3:27659242-27659261 None:intergenic 40.0%
!! GGTATTGCTGGTATTGGTAT+TGG - chr6.3:27660047-27660066 None:intergenic 40.0%
!! GGTATTGGTGGAAAATGTGT+AGG - chr6.3:27659450-27659469 None:intergenic 40.0%
!! GTTGGAGTTTGAACTTGTTG+CGG - chr6.3:27660074-27660093 None:intergenic 40.0%
!! GTTGTTGATGATGGTATTGG+TGG - chr6.3:27659462-27659481 None:intergenic 40.0%
!! TGTGTTTGTGTTTGAGTTGG+TGG - chr6.3:27659288-27659307 None:intergenic 40.0%
AACAAGAGGAGTTGCACAAG+AGG + chr6.3:27659703-27659722 MS.gene80356:CDS 45.0%
ACAGCAGCAAGTGACGAATA+TGG + chr6.3:27660184-27660203 MS.gene80356:CDS 45.0%
AGAACCAAAGAAGGAAGAGG+TGG + chr6.3:27659171-27659190 MS.gene80356:CDS 45.0%
AGGGGTGGGAATAGTACTAA+TGG - chr6.3:27659316-27659335 None:intergenic 45.0%
AGTGCAACAACAAACAGCAC+CGG + chr6.3:27660100-27660119 MS.gene80356:CDS 45.0%
ATACAGGCAGAAGAACAAGG+TGG + chr6.3:27660520-27660539 MS.gene80356:CDS 45.0%
ATCTAGGTGGCCAAAAACTG+AGG + chr6.3:27660256-27660275 MS.gene80356:CDS 45.0%
ATGGACCTAAAGGGCCATTA+TGG + chr6.3:27660330-27660349 MS.gene80356:CDS 45.0%
CAGATTTCAGAAGGTGGCAT+TGG - chr6.3:27658125-27658144 None:intergenic 45.0%
CCATTATGGGAGGAGATTTC+AGG + chr6.3:27660344-27660363 MS.gene80356:CDS 45.0%
CCTGAAATCTCCTCCCATAA+TGG - chr6.3:27660347-27660366 None:intergenic 45.0%
GAAACGACAACAATGGAAGG+GGG + chr6.3:27659609-27659628 MS.gene80356:CDS 45.0%
GAAGAGGAAGAGGAAATGGA+AGG + chr6.3:27659644-27659663 MS.gene80356:CDS 45.0%
GAGGAAACGACAACAATGGA+AGG + chr6.3:27659606-27659625 MS.gene80356:CDS 45.0%
GGAAACGACAACAATGGAAG+GGG + chr6.3:27659608-27659627 MS.gene80356:CDS 45.0%
GGAAGAGGTGGTAAATCAGA+TGG + chr6.3:27659183-27659202 MS.gene80356:CDS 45.0%
GGAGGTTGTTGAGAAACAAG+AGG + chr6.3:27659689-27659708 MS.gene80356:CDS 45.0%
GGGGTGGGAATAGTACTAAT+GGG - chr6.3:27659315-27659334 None:intergenic 45.0%
TGCATGCAAGTTCATCTAGG+TGG + chr6.3:27660243-27660262 MS.gene80356:CDS 45.0%
TGGACCTAAAGGGCCATTAT+GGG + chr6.3:27660331-27660350 MS.gene80356:CDS 45.0%
TGGTGGAAAATGTGTAGGGT+TGG - chr6.3:27659445-27659464 None:intergenic 45.0%
TTCGGAGGAAACGACAACAA+TGG + chr6.3:27659602-27659621 MS.gene80356:CDS 45.0%
! GCCCTTTAGGTCCATTTTCT+TGG - chr6.3:27660325-27660344 None:intergenic 45.0%
! GCCTGTATAGAGCATCAAGT+TGG - chr6.3:27660508-27660527 None:intergenic 45.0%
! GGTGTAGGTATTGCTGGTAT+TGG - chr6.3:27660053-27660072 None:intergenic 45.0%
! TCAGGTGATGATGAAAGAGG+GGG + chr6.3:27657866-27657885 MS.gene80356:CDS 45.0%
! TTAGCGAATGAAGATGGTGG+GGG - chr6.3:27659537-27659556 None:intergenic 45.0%
!! GAGGGTGTGGATAGAAGTTT+TGG + chr6.3:27657908-27657927 MS.gene80356:CDS 45.0%
!! GCTGGTATTGGTATTGGCAA+TGG - chr6.3:27660041-27660060 None:intergenic 45.0%
!! GGTGTGGATAGAAGTTTTGG+TGG + chr6.3:27657911-27657930 MS.gene80356:CDS 45.0%
!! GTTGGTGGTGGTGTTATTGA+AGG - chr6.3:27659273-27659292 None:intergenic 45.0%
!! GTTTGAACTTGTTGCGGTGT+AGG - chr6.3:27660068-27660087 None:intergenic 45.0%
!! GTTTGTGTTTGAGTTGGTGG+TGG - chr6.3:27659285-27659304 None:intergenic 45.0%
!! TGTAGTTGATGGAACAGTGG+TGG - chr6.3:27659394-27659413 None:intergenic 45.0%
!! GAAAAATTAAATTAATAAAT+GGG - chr6.3:27658229-27658248 None:intergenic 5.0%
!! TGAAAAATTAAATTAATAAA+TGG - chr6.3:27658230-27658249 None:intergenic 5.0%
ACATCAGCAGCAGCAACAAC+AGG + chr6.3:27660151-27660170 MS.gene80356:CDS 50.0%
ACCTAAAGGGCCATTATGGG+AGG + chr6.3:27660334-27660353 MS.gene80356:CDS 50.0%
AGCATTCTCAGCATCCTCCA+TGG - chr6.3:27660663-27660682 None:intergenic 50.0%
AGGCAGAAGAACAAGGTGGA+GGG + chr6.3:27660524-27660543 MS.gene80356:CDS 50.0%
CTAGGGGACTCAACAGTGTT+GGG + chr6.3:27657779-27657798 MS.gene80356:CDS 50.0%
GAAGCTGTAGTTGTAGGGGT+GGG - chr6.3:27659330-27659349 None:intergenic 50.0%
GACGGAAGAGGAAGAGGAAA+TGG + chr6.3:27659640-27659659 MS.gene80356:CDS 50.0%
GATGACGAGGACGAAGATGA+AGG + chr6.3:27660740-27660759 MS.gene80356:CDS 50.0%
GGAGGTTGCAAGAGATGCAA+AGG + chr6.3:27659781-27659800 MS.gene80356:CDS 50.0%
GGAGTTGCACAAGAGGTTCT+TGG + chr6.3:27659710-27659729 MS.gene80356:CDS 50.0%
GTGCTAGAGAAGAAGCTTGG+AGG + chr6.3:27659763-27659782 MS.gene80356:CDS 50.0%
GTGGCCAAAAACTGAGGTTG+AGG + chr6.3:27660262-27660281 MS.gene80356:CDS 50.0%
TCCTCCCATAATGGCCCTTT+AGG - chr6.3:27660338-27660357 None:intergenic 50.0%
TGAAGCTGTAGTTGTAGGGG+TGG - chr6.3:27659331-27659350 None:intergenic 50.0%
TGCTGCTGATGTTGTGGCAA+CGG - chr6.3:27660143-27660162 None:intergenic 50.0%
TGTTGCTGCTGCTGATGTTG+TGG - chr6.3:27660149-27660168 None:intergenic 50.0%
TTCGTCTTCGACTTCTTCGG+AGG + chr6.3:27659587-27659606 MS.gene80356:CDS 50.0%
TTGATCCACCACCACCATCA+TGG - chr6.3:27657844-27657863 None:intergenic 50.0%
! AACGATGATGGCACCGTTGA+TGG + chr6.3:27660574-27660593 MS.gene80356:CDS 50.0%
! GACTCAACAGTGTTGGGAAC+AGG + chr6.3:27657785-27657804 MS.gene80356:CDS 50.0%
! GAGGTGCTAGAGAAGAAGCT+TGG + chr6.3:27659760-27659779 MS.gene80356:CDS 50.0%
! GTTGATGGAACAGTGGTGGT+AGG - chr6.3:27659390-27659409 None:intergenic 50.0%
! TCAACAGTGTTGGGAACAGG+AGG + chr6.3:27657788-27657807 MS.gene80356:CDS 50.0%
! TGTTGCGGTGTAGGTATTGC+TGG - chr6.3:27660059-27660078 None:intergenic 50.0%
!! GGTATTGGCAATGGTACTGC+TGG - chr6.3:27660032-27660051 None:intergenic 50.0%
!! GGTGGTGGTGGATCAATTTC+AGG + chr6.3:27657848-27657867 MS.gene80356:CDS 50.0%
!! TGATGACGGTGATGATGACG+AGG + chr6.3:27660727-27660746 MS.gene80356:CDS 50.0%
!! TTGAGGTGGTGGTGGTTTGT+TGG - chr6.3:27660789-27660808 None:intergenic 50.0%
CAGGCAGAAGAACAAGGTGG+AGG + chr6.3:27660523-27660542 MS.gene80356:CDS 55.0%
CGAATCGACGGAAGAGGAAG+AGG + chr6.3:27659634-27659653 MS.gene80356:CDS 55.0%
GATGGAACAGTGGTGGTAGG+AGG - chr6.3:27659387-27659406 None:intergenic 55.0%
GCAATGGCCTCCACAACAAC+AGG + chr6.3:27660610-27660629 MS.gene80356:CDS 55.0%
GCAGCAGCAACAACAGGTTC+AGG + chr6.3:27660157-27660176 MS.gene80356:CDS 55.0%
GCAGGAGCTTACCATGATGG+TGG + chr6.3:27657830-27657849 MS.gene80356:CDS 55.0%
GCTAGGGGACTCAACAGTGT+TGG + chr6.3:27657778-27657797 MS.gene80356:CDS 55.0%
GGAGCAGGAGCTTACCATGA+TGG + chr6.3:27657827-27657846 MS.gene80356:CDS 55.0%
GGCAATGGTACTGCTGGTAC+TGG - chr6.3:27660026-27660045 None:intergenic 55.0%
TGCTCCGGCTGAACCATCAA+CGG - chr6.3:27660590-27660609 None:intergenic 55.0%
TGGAAGGGGGATCGAATCGA+CGG + chr6.3:27659622-27659641 MS.gene80356:CDS 55.0%
! ACAGTGTTGGGAACAGGAGG+AGG + chr6.3:27657791-27657810 MS.gene80356:CDS 55.0%
! GGTGATGATGAAAGAGGGGG+TGG + chr6.3:27657869-27657888 MS.gene80356:CDS 55.0%
! TCAACAACAACAAGCGCCGC+AGG + chr6.3:27659944-27659963 MS.gene80356:CDS 55.0%
!! AACAGCACCGGTCATTCCTC+AGG + chr6.3:27660112-27660131 MS.gene80356:CDS 55.0%
!! AATGGTGCTGCTGCTGGTGT+CGG - chr6.3:27660002-27660021 None:intergenic 55.0%
!! GCTTACCATGATGGTGGTGG+TGG + chr6.3:27657836-27657855 MS.gene80356:CDS 55.0%
!! GGAGCTTACCATGATGGTGG+TGG + chr6.3:27657833-27657852 MS.gene80356:CDS 55.0%
!! GGGGTTGGTGTAGTTTCCTG+CGG - chr6.3:27659963-27659982 None:intergenic 55.0%
!! GGTACTGCTGGTACTGGCAA+TGG - chr6.3:27660020-27660039 None:intergenic 55.0%
!! TCGGTGTCGGTGTTGGTGTT+GGG - chr6.3:27659983-27660002 None:intergenic 55.0%
!! TGCGGCGCTTGTTGTTGTTG+AGG - chr6.3:27659945-27659964 None:intergenic 55.0%
GAGGCCTGAGGGAACGATGA+TGG + chr6.3:27660562-27660581 MS.gene80356:CDS 60.0%
GGCGAAGCCTGAGGAATGAC+CGG - chr6.3:27660122-27660141 None:intergenic 60.0%
GGGGATCGAATCGACGGAAG+AGG + chr6.3:27659628-27659647 MS.gene80356:CDS 60.0%
TGATCGGCCGTGTCGAGATG+AGG + chr6.3:27660688-27660707 MS.gene80356:CDS 60.0%
TGGTTCAGCCGGAGCAGCAA+TGG + chr6.3:27660594-27660613 MS.gene80356:CDS 60.0%
TGTGGAGGCCATTGCTGCTC+CGG - chr6.3:27660605-27660624 None:intergenic 60.0%
! GGTGCCATCATCGTTCCCTC+AGG - chr6.3:27660569-27660588 None:intergenic 60.0%
!! ACTGGCAATGGTGCTGCTGC+TGG - chr6.3:27660008-27660027 None:intergenic 60.0%
!! CGGTGTCGGTGTTGGTGTTG+GGG - chr6.3:27659982-27660001 None:intergenic 60.0%
!! GGCACCGTTGATGGTTCAGC+CGG + chr6.3:27660583-27660602 MS.gene80356:CDS 60.0%
!! GTCGGTGTCGGTGTTGGTGT+TGG - chr6.3:27659984-27660003 None:intergenic 60.0%
!! GTCGGTGTTGGTGTTGGGGT+TGG - chr6.3:27659978-27659997 None:intergenic 60.0%
AGCCAGGGCGGATGTGACCA+TGG + chr6.3:27660643-27660662 MS.gene80356:CDS 65.0%
CAGGGCGGATGTGACCATGG+AGG + chr6.3:27660646-27660665 MS.gene80356:CDS 65.0%
CTCCATGGTCACATCCGCCC+TGG - chr6.3:27660648-27660667 None:intergenic 65.0%
GGAACAGGAGGAGGCAGCTC+AGG + chr6.3:27657800-27657819 MS.gene80356:CDS 65.0%
GGAGGAGGCAGCTCAGGAGA+TGG + chr6.3:27657806-27657825 MS.gene80356:CDS 65.0%
GGCAGCTCAGGAGATGGAGC+AGG + chr6.3:27657812-27657831 MS.gene80356:CDS 65.0%
GGTGGCGCCTGTTGTTGTGG+AGG - chr6.3:27660620-27660639 None:intergenic 65.0%
TGTGGCAACGGCGAAGCCTG+AGG - chr6.3:27660131-27660150 None:intergenic 65.0%
! GCTGGTGGCGCCTGTTGTTG+TGG - chr6.3:27660623-27660642 None:intergenic 65.0%
!! GCTGCTGCTGGTGTCGGTGT+CGG - chr6.3:27659996-27660015 None:intergenic 65.0%
!! GCTGGTGTCGGTGTCGGTGT+TGG - chr6.3:27659990-27660009 None:intergenic 65.0%
AACAGGCGCCACCAGCAGCC+AGG + chr6.3:27660627-27660646 MS.gene80356:CDS 70.0%
ACAGGCGCCACCAGCAGCCA+GGG + chr6.3:27660628-27660647 MS.gene80356:CDS 70.0%
ACATCCGCCCTGGCTGCTGG+TGG - chr6.3:27660638-27660657 None:intergenic 70.0%
AGCTGCTGCGTCGAGGCCTG+AGG + chr6.3:27660550-27660569 MS.gene80356:CDS 70.0%
AGGGTGCAGCTGCTGCGTCG+AGG + chr6.3:27660543-27660562 MS.gene80356:CDS 70.0%
GCTGCTGCGTCGAGGCCTGA+GGG + chr6.3:27660551-27660570 MS.gene80356:CDS 70.0%
GTCACATCCGCCCTGGCTGC+TGG - chr6.3:27660641-27660660 None:intergenic 70.0%
! GGCGCCACCAGCAGCCAGGG+CGG + chr6.3:27660631-27660650 MS.gene80356:CDS 80.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 27657776 27660811 27657776 ID=MS.gene80356
chr6.3 mRNA 27657776 27660811 27657776 ID=MS.gene80356.t1;Parent=MS.gene80356
chr6.3 exon 27657776 27658044 27657776 ID=MS.gene80356.t1.exon1;Parent=MS.gene80356.t1
chr6.3 CDS 27657776 27658044 27657776 ID=cds.MS.gene80356.t1;Parent=MS.gene80356.t1
chr6.3 exon 27659092 27660811 27659092 ID=MS.gene80356.t1.exon2;Parent=MS.gene80356.t1
chr6.3 CDS 27659092 27660811 27659092 ID=cds.MS.gene80356.t1;Parent=MS.gene80356.t1
Gene Sequence

>MS.gene80356

ATGCTAGGGGACTCAACAGTGTTGGGAACAGGAGGAGGCAGCTCAGGAGATGGAGCAGGAGCTTACCATGATGGTGGTGGTGGATCAATTTCAGGTGATGATGAAAGAGGGGGTGGAAGTAGTAGAAATGAAGAGGGTGTGGATAGAAGTTTTGGTGGTAATAGATGGCCAAGACAAGAAACAGTTGCTTTGTTGAAGATAAGGTCTGATATGGATGTTGCTTTCAAAGATGCAAGTGTTAAAGGTCCCTTATGGGATGAAGTTTCAAGGTTAGTCAAAAAAAAAAAATTCAATTTTTTTCATTTTTTTGATTTTAATTATTTGAGATTGTATTCAACTTGTTAATCCAATGCCACCTTCTGAAATCTGAAAAAAAAATTCACTTTGAGTTCATGTTTTGTGTATTAATTTTTTTTTTTCAATGTGTTTCAATTTTCATTTCATCTCAATCCCATTTATTAATTTAATTTTTCAATTTTTGATATATTTTTTACTTGATTCAATGCCACCTTGAGAAATCTGAATTATTGAATGTGTTTCAATTTTGATTTTGATTTCACTTCAATTTCATATTTTTTTTATCTTTGATTTGGACTTGTTTTCATGTAAAGATGAATTTTTTAATTTTTTTGTTATTATTGAATTATTGAATTGATTATTGAAGTTCCTTCATGTATATTTTTAATTTAAAGATAAGTACTGTTTATGAAAAATTAAATAATTTAGAGATGTGTGCAAGCATCTCTCTATGACTCTATCTATCTATCTATGATATGATGACTTTGATGTGATTTGCTTTTCTGACATTCATTTTGATACATGTTGTATTTTTACCAAATAAAGTTACAAACTTGAATTGAGTCACCAAAAAGCAAAGATTTTCTTCCTCCCTTATTTCAGTTACCCATTTTTAGTGTTGAAAAATAAAAATATGATGGTAGATTACAAAATCTTTGTTCACACTTTGGTTCATGTGTTTTCCATGAGAAACAAACTTTTTAATTTTTTCAACATTTTACGCTATTTAATTTCTTGTCATTATTAAAAAAAAATCTTCACTCCAATTACATCAACTTTGATATATAATAATTTTTTATTCACCTCACCCTTATCTTAATCACTTAAGTTACTTGCTCCATTTCAATTCTTTGGTTGTCTAAATTGATTTATTTATTTTTGACCCAATTCATTTATTATCTTATGCACCAATTAATTGCTTCTTATCCTAAAGATTTAATGAGATAAGAAAAAGCCAGAAATTTTATTTTTTTTCTTTAATTAATTTTGTCTTTATTTTTGGGTACTTTGGCTAATAGGAAAATGGCAGATCTTGGGTATCAAAGAAACTCCAAAAAATGCAAAGAAAAATTTGAGAATGTTTACAAATATCACAAGAGAACCAAAGAAGGAAGAGGTGGTAAATCAGATGGTAAAACTTATAGATTCTTTGATCAATTACAAGCCCTTGAAAACAACCCTTCAATGCATCAATCACCTTCAATAACACCACCACCAACTCAAACACAAACACAAACACCCATTAGTACTATTCCCACCCCTACAACTACAGCTTCATTTCTATCTGCACCTCCATTGTTAAACTCTACACCTCCTACCACCACTGTTCCATCAACTACACTTCCAATTCCAATGTCTTACACACAATCCAACCCTACACATTTTCCACCAATACCATCATCAACAACAACAACAAACCCTACAAATACTAATAACCCTCTTCCTCAAATTACTATTACACCCCCACCATCTTCATTCGCTAATATTTCAGCGGATTTCTTCTCGAATTCAAGTTCGTCTTCGACTTCTTCGGAGGAAACGACAACAATGGAAGGGGGATCGAATCGACGGAAGAGGAAGAGGAAATGGAAGGATTTCTTTGAGAGACTAATGAAGGAGGTTGTTGAGAAACAAGAGGAGTTGCACAAGAGGTTCTTGGAAGCTATAGAGAAAAGAGAAAGAGAGAGAGGTGCTAGAGAAGAAGCTTGGAGGTTGCAAGAGATGCAAAGGATTAATAGAGAAAGGGAAATTCTTGCTCAAGAGAGATCTATTGCTGCTACTAAAGATGCTGCTGTTATGGCTTTTTTGCAGAAGATTGCTGAACAACAAAATCTTGTTCCTCCTGTATTGAACAACAACACCGTCATTCCTCAACAACAACAAGCGCCGCAGGAAACTACACCAACCCCAACACCAACACCGACACCGACACCAGCAGCAGCACCATTGCCAGTACCAGCAGTACCATTGCCAATACCAATACCAGCAATACCTACACCGCAACAAGTTCAAACTCCAACTGTAACAGTGCAACAACAAACAGCACCGGTCATTCCTCAGGCTTCGCCGTTGCCACAACATCAGCAGCAGCAACAACAGGTTCAGGTACAACAGCAGCAAGTGACGAATATGGAAGTTGCGAAAAGTGACAACAATGGTGAGAGTATGATGCATGCAAGTTCATCTAGGTGGCCAAAAACTGAGGTTGAGGCATTGATAAAGCTGAGAACAACTCTTGATATGAAGTACCAAGAAAATGGACCTAAAGGGCCATTATGGGAGGAGATTTCAGGTTTGATGAAGAAAATGGGGTACAATAGGAATGCAAAGAGGTGCAAAGAGAAATGGGAGAACATCAACAAGTATTTTAAGAAAGTGAAGGAAAGCAACAAGAAGAGGCCAGAAGATTCAAAGACATGTCCTTACTTTCACCAACTTGATGCTCTATACAGGCAGAAGAACAAGGTGGAGGGTGCAGCTGCTGCGTCGAGGCCTGAGGGAACGATGATGGCACCGTTGATGGTTCAGCCGGAGCAGCAATGGCCTCCACAACAACAGGCGCCACCAGCAGCCAGGGCGGATGTGACCATGGAGGATGCTGAGAATGCTGAGAATGATCGGCCGTGTCGAGATGAGGAAGAATATTATGACGATGATGACGGTGATGATGACGAGGACGAAGATGAAGGTAGTGACAATTATGAAGTGGTTGCCAACAAACCACCACCACCTCAATAA
Protein sequence

>MS.gene80356.t1

MLGDSTVLGTGGGSSGDGAGAYHDGGGGSISGDDERGGGSSRNEEGVDRSFGGNRWPRQETVALLKIRSDMDVAFKDASVKGPLWDEVSRKMADLGYQRNSKKCKEKFENVYKYHKRTKEGRGGKSDGKTYRFFDQLQALENNPSMHQSPSITPPPTQTQTQTPISTIPTPTTTASFLSAPPLLNSTPPTTTVPSTTLPIPMSYTQSNPTHFPPIPSSTTTTNPTNTNNPLPQITITPPPSSFANISADFFSNSSSSSTSSEETTTMEGGSNRRKRKRKWKDFFERLMKEVVEKQEELHKRFLEAIEKRERERGAREEAWRLQEMQRINREREILAQERSIAATKDAAVMAFLQKIAEQQNLVPPVLNNNTVIPQQQQAPQETTPTPTPTPTPTPAAAPLPVPAVPLPIPIPAIPTPQQVQTPTVTVQQQTAPVIPQASPLPQHQQQQQQVQVQQQQVTNMEVAKSDNNGESMMHASSSRWPKTEVEALIKLRTTLDMKYQENGPKGPLWEEISGLMKKMGYNRNAKRCKEKWENINKYFKKVKESNKKRPEDSKTCPYFHQLDALYRQKNKVEGAAAASRPEGTMMAPLMVQPEQQWPPQQQAPPAARADVTMEDAENAENDRPCRDEEEYYDDDDGDDDEDEDEGSDNYEVVANKPPPPQ