Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80568.t1 | XP_003609133.2 | 97 | 99 | 3 | 0 | 1 | 99 | 485 | 583 | 6.10E-47 | 196.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80568.t1 | Q84T03 | 62.4 | 101 | 35 | 2 | 1 | 98 | 489 | 589 | 1.9e-28 | 126.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80568.t1 | G7JT08 | 97.0 | 99 | 3 | 0 | 1 | 99 | 485 | 583 | 4.4e-47 | 196.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene060830 | MS.gene80568 | 0.830751 | 2.46E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80568.t1 | MTR_4g112320 | 96.970 | 99 | 3 | 0 | 1 | 99 | 303 | 401 | 6.16e-66 | 203 |
| MS.gene80568.t1 | MTR_4g112340 | 96.970 | 99 | 3 | 0 | 1 | 99 | 485 | 583 | 7.30e-65 | 205 |
| MS.gene80568.t1 | MTR_2g055850 | 64.103 | 78 | 26 | 1 | 1 | 76 | 478 | 555 | 1.32e-28 | 107 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80568.t1 | AT3G18600 | 57.471 | 87 | 37 | 0 | 1 | 87 | 475 | 561 | 6.46e-28 | 105 |
| MS.gene80568.t1 | AT5G65900 | 50.685 | 73 | 36 | 0 | 1 | 73 | 541 | 613 | 2.74e-18 | 78.6 |
Find 15 sgRNAs with CRISPR-Local
Find 70 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTGCATTGTGTAGGAGAATT+TGG | 0.238966 | 8.4:-17258123 | None:intergenic |
| TTCAATCCTAACAATCCTTA+TGG | 0.244240 | 8.4:-17256947 | MS.gene80568:CDS |
| CGCTTTCCATAAGGATTGTT+AGG | 0.285269 | 8.4:+17256941 | None:intergenic |
| TTTATGTTTAGACTCACATT+TGG | 0.413125 | 8.4:+17257022 | None:intergenic |
| AAGAAAACTCGTAGAGTTGA+TGG | 0.420262 | 8.4:-17256983 | MS.gene80568:CDS |
| TCGGCATTTCGCTTTCCATA+AGG | 0.452668 | 8.4:+17256932 | None:intergenic |
| TGTTCAACGCCTTGATATGC+AGG | 0.485964 | 8.4:-17258006 | MS.gene80568:intron |
| ATGTTTAGACTCACATTTGG+AGG | 0.506185 | 8.4:+17257025 | None:intergenic |
| CACAAACTGCCGCTTATCAT+CGG | 0.519919 | 8.4:+17256913 | None:intergenic |
| AGAGTTGTACCTGCATATCA+AGG | 0.540807 | 8.4:+17257997 | None:intergenic |
| AGAGTTGATGGAAGCAGACA+TGG | 0.562166 | 8.4:-17256971 | MS.gene80568:CDS |
| AGCGAAATGCCGATGATAAG+CGG | 0.563676 | 8.4:-17256922 | MS.gene80568:CDS |
| TGTTTAGACTCACATTTGGA+GGG | 0.580998 | 8.4:+17257026 | None:intergenic |
| GTACAATTCACATTCTTCGA+AGG | 0.594754 | 8.4:-17258039 | MS.gene80568:CDS |
| ACATAAACGTCAACAAGCAA+AGG | 0.600961 | 8.4:-17257006 | MS.gene80568:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTATTTTTTGAACAAAA+TGG | - | chr8.4:17256921-17256940 | MS.gene80568:CDS | 10.0% |
| !! | GATGAACTTATCTTAAAAAA+TGG | + | chr8.4:17257340-17257359 | None:intergenic | 20.0% |
| !! | TCCACTATTAAAATAAAACA+AGG | + | chr8.4:17257611-17257630 | None:intergenic | 20.0% |
| !! | TGATTTAAATTATCAAGTAG+AGG | + | chr8.4:17257130-17257149 | None:intergenic | 20.0% |
| !!! | AATTTTGATTAAAAGCTTCA+AGG | - | chr8.4:17257181-17257200 | MS.gene80568:intron | 20.0% |
| !!! | TCCTTGTTTTATTTTAATAG+TGG | - | chr8.4:17257607-17257626 | MS.gene80568:intron | 20.0% |
| ! | AAATGAAAAGATGATTTCTC+CGG | + | chr8.4:17257734-17257753 | None:intergenic | 25.0% |
| ! | AATTATCAAGTAGAGGAATA+AGG | + | chr8.4:17257123-17257142 | None:intergenic | 25.0% |
| ! | AATTTAAATCACCTGTTACT+GGG | - | chr8.4:17257139-17257158 | MS.gene80568:intron | 25.0% |
| ! | AGTTAGATCTGAAAAAATTG+GGG | - | chr8.4:17257400-17257419 | MS.gene80568:intron | 25.0% |
| ! | ATTATCAAGTAGAGGAATAA+GGG | + | chr8.4:17257122-17257141 | None:intergenic | 25.0% |
| ! | GAGTTAGATCTGAAAAAATT+GGG | - | chr8.4:17257399-17257418 | MS.gene80568:intron | 25.0% |
| ! | GGAAAAGAATGAATTATTTC+AGG | - | chr8.4:17257639-17257658 | MS.gene80568:intron | 25.0% |
| ! | TAATTTAAATCACCTGTTAC+TGG | - | chr8.4:17257138-17257157 | MS.gene80568:intron | 25.0% |
| ! | TCAGGATATTCATTTATTTC+AGG | - | chr8.4:17257657-17257676 | MS.gene80568:intron | 25.0% |
| ! | TGAGTTAGATCTGAAAAAAT+TGG | - | chr8.4:17257398-17257417 | MS.gene80568:intron | 25.0% |
| ! | TTGGAATATAGCATATTTGA+AGG | + | chr8.4:17257795-17257814 | None:intergenic | 25.0% |
| ! | TTTATGTTTAGACTCACATT+TGG | + | chr8.4:17257995-17258014 | None:intergenic | 25.0% |
| !! | CTGAATGAAATCAATTTTCT+GGG | + | chr8.4:17257568-17257587 | None:intergenic | 25.0% |
| !! | TCTGAATGAAATCAATTTTC+TGG | + | chr8.4:17257569-17257588 | None:intergenic | 25.0% |
| !!! | CATGATGTTATCTTTTAACT+AGG | + | chr8.4:17257309-17257328 | None:intergenic | 25.0% |
| !!! | TTTTAATAGTGGAAGAAACA+CGG | - | chr8.4:17257618-17257637 | MS.gene80568:intron | 25.0% |
| ACAAATATGATACTTCTCTC+TGG | + | chr8.4:17257865-17257884 | None:intergenic | 30.0% | |
| AGTGAGAAAGTTAAGTTAAG+CGG | - | chr8.4:17257504-17257523 | MS.gene80568:intron | 30.0% | |
| ATTAAAAGCTTCAAGGATAG+GGG | - | chr8.4:17257188-17257207 | MS.gene80568:intron | 30.0% | |
| CTAAAGTCACCAAATAGTAA+AGG | + | chr8.4:17257439-17257458 | None:intergenic | 30.0% | |
| CTCAAAGTATAGAAACAAAC+TGG | + | chr8.4:17257936-17257955 | None:intergenic | 30.0% | |
| GATTAAAAGCTTCAAGGATA+GGG | - | chr8.4:17257187-17257206 | MS.gene80568:intron | 30.0% | |
| GATTCTCTACCTTTACTATT+TGG | - | chr8.4:17257427-17257446 | MS.gene80568:intron | 30.0% | |
| TGATTAAAAGCTTCAAGGAT+AGG | - | chr8.4:17257186-17257205 | MS.gene80568:intron | 30.0% | |
| TTCAATCCTAACAATCCTTA+TGG | - | chr8.4:17258067-17258086 | MS.gene80568:CDS | 30.0% | |
| ! | ATGAAATCAATTTTCTGGGT+TGG | + | chr8.4:17257564-17257583 | None:intergenic | 30.0% |
| ! | GTATTTTGTAGATAGATCGA+TGG | - | chr8.4:17257460-17257479 | MS.gene80568:intron | 30.0% |
| ! | TGAAATCAATTTTCTGGGTT+GGG | + | chr8.4:17257563-17257582 | None:intergenic | 30.0% |
| !! | TTTGTTTCTATACTTTGAGC+AGG | - | chr8.4:17257937-17257956 | MS.gene80568:intron | 30.0% |
| AAGAAAACTCGTAGAGTTGA+TGG | - | chr8.4:17258031-17258050 | MS.gene80568:CDS | 35.0% | |
| AAGGATAGGGGTAAAATGAA+TGG | - | chr8.4:17257200-17257219 | MS.gene80568:intron | 35.0% | |
| ACATAAACGTCAACAAGCAA+AGG | - | chr8.4:17258008-17258027 | MS.gene80568:CDS | 35.0% | |
| ATATAAGAGTTCCCAGTAAC+AGG | + | chr8.4:17257153-17257172 | None:intergenic | 35.0% | |
| ATCTAGATAGAGACTGGAAA+GGG | - | chr8.4:17257368-17257387 | MS.gene80568:intron | 35.0% | |
| ATGTTTAGACTCACATTTGG+AGG | + | chr8.4:17257992-17258011 | None:intergenic | 35.0% | |
| CAAACATGCATCAAGTACTA+TGG | - | chr8.4:17257812-17257831 | MS.gene80568:intron | 35.0% | |
| CAGCTTTCACTGAGAATTTA+TGG | + | chr8.4:17257758-17257777 | None:intergenic | 35.0% | |
| CATAGTACTTGATGCATGTT+TGG | + | chr8.4:17257814-17257833 | None:intergenic | 35.0% | |
| CTGAACACAATTAATCAGAG+CGG | - | chr8.4:17257244-17257263 | MS.gene80568:intron | 35.0% | |
| GATCTGAAAAAATTGGGGAA+AGG | - | chr8.4:17257405-17257424 | MS.gene80568:intron | 35.0% | |
| GGGTAAAATGAATGGTTGAT+TGG | - | chr8.4:17257208-17257227 | MS.gene80568:intron | 35.0% | |
| GTACAATTCACATTCTTCGA+AGG | - | chr8.4:17256975-17256994 | MS.gene80568:CDS | 35.0% | |
| GTGAGAAAGTTAAGTTAAGC+GGG | - | chr8.4:17257505-17257524 | MS.gene80568:intron | 35.0% | |
| TGTTTAGACTCACATTTGGA+GGG | + | chr8.4:17257991-17258010 | None:intergenic | 35.0% | |
| TTGTAGATCTAGATAGAGAC+TGG | - | chr8.4:17257362-17257381 | MS.gene80568:intron | 35.0% | |
| AGAGTTGTACCTGCATATCA+AGG | + | chr8.4:17257020-17257039 | None:intergenic | 40.0% | |
| AGCGGCAACACATAGATTAA+AGG | - | chr8.4:17257262-17257281 | MS.gene80568:intron | 40.0% | |
| AGTTAAGTTAAGCGGGAGAT+AGG | - | chr8.4:17257512-17257531 | MS.gene80568:intron | 40.0% | |
| CGCTTTCCATAAGGATTGTT+AGG | + | chr8.4:17258076-17258095 | None:intergenic | 40.0% | |
| GATCTAGATAGAGACTGGAA+AGG | - | chr8.4:17257367-17257386 | MS.gene80568:intron | 40.0% | |
| GTTAAGTTAAGCGGGAGATA+GGG | - | chr8.4:17257513-17257532 | MS.gene80568:intron | 40.0% | |
| AGCGAAATGCCGATGATAAG+CGG | - | chr8.4:17258092-17258111 | MS.gene80568:CDS | 45.0% | |
| TCGGCATTTCGCTTTCCATA+AGG | + | chr8.4:17258085-17258104 | None:intergenic | 45.0% | |
| TGTTCAACGCCTTGATATGC+AGG | - | chr8.4:17257008-17257027 | MS.gene80568:CDS | 45.0% | |
| ! | CACAAACTGCCGCTTATCAT+CGG | + | chr8.4:17258104-17258123 | None:intergenic | 45.0% |
| !! | AGAGTTGATGGAAGCAGACA+TGG | - | chr8.4:17258043-17258062 | MS.gene80568:CDS | 45.0% |
| AAACGCCCCTGTGTATTGAG+TGG | - | chr8.4:17257704-17257723 | MS.gene80568:intron | 50.0% | |
| CTGTGTATTGAGTGGTAGGC+CGG | - | chr8.4:17257712-17257731 | MS.gene80568:intron | 50.0% | |
| GCCTACCACTCAATACACAG+GGG | + | chr8.4:17257712-17257731 | None:intergenic | 50.0% | |
| GGCCTACCACTCAATACACA+GGG | + | chr8.4:17257713-17257732 | None:intergenic | 50.0% | |
| CGGCCTACCACTCAATACAC+AGG | + | chr8.4:17257714-17257733 | None:intergenic | 55.0% | |
| GATAGGGACGAAGTCACCAG+AGG | - | chr8.4:17257529-17257548 | MS.gene80568:intron | 55.0% | |
| GCCCCTGTGTATTGAGTGGT+AGG | - | chr8.4:17257708-17257727 | MS.gene80568:intron | 55.0% | |
| GGGTTGGGATTGTGTACCTC+TGG | + | chr8.4:17257548-17257567 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 17256904 | 17258132 | 17256904 | ID=MS.gene80568 |
| chr8.4 | mRNA | 17256904 | 17258132 | 17256904 | ID=MS.gene80568.t1;Parent=MS.gene80568 |
| chr8.4 | exon | 17258007 | 17258132 | 17258007 | ID=MS.gene80568.t1.exon1;Parent=MS.gene80568.t1 |
| chr8.4 | CDS | 17258007 | 17258132 | 17258007 | ID=cds.MS.gene80568.t1;Parent=MS.gene80568.t1 |
| chr8.4 | exon | 17256904 | 17257077 | 17256904 | ID=MS.gene80568.t1.exon2;Parent=MS.gene80568.t1 |
| chr8.4 | CDS | 17256904 | 17257077 | 17256904 | ID=cds.MS.gene80568.t1;Parent=MS.gene80568.t1 |
| Gene Sequence |
| Protein sequence |
