AlfalfaGEDB | MS.gene80863

M. sativa cultivar XinJiangDaYe / MS.gene80863

KEGG: K17499
GO: GO:0003674 GO:0003824
PFAM: PF00481.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80863.t1	XP_024631053.1	95.4	65	3	0	18	82	232	296	8.10E-27	129.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80863.t1	Q6ETK3	66.7	60	20	0	18	77	232	291	3.7e-20	98.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80863.t1	G7I891	95.4	65	3	0	18	82	232	296	5.9e-27	129.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056174	MS.gene80863	0.803212	3.88E-49	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80863.t1	MTR_1g014190	95.385	65	3	0	18	82	232	296	1.36e-38	131
MS.gene80863.t1	MTR_1g014190	95.385	65	3	0	18	82	270	334	3.74e-38	131
MS.gene80863.t1	MTR_3g105880	76.667	60	14	0	18	77	232	291	2.83e-29	106
MS.gene80863.t1	MTR_3g105880	72.308	65	18	0	18	82	232	296	7.32e-29	105
MS.gene80863.t1	MTR_4g007440	66.667	60	20	0	18	77	232	291	3.55e-25	96.3
MS.gene80863.t1	MTR_4g123080	50.000	60	27	1	18	77	142	198	2.77e-12	60.5
MS.gene80863.t1	MTR_4g123080	50.000	60	27	1	18	77	255	311	3.56e-12	60.5
MS.gene80863.t1	MTR_4g123080	50.000	60	27	1	18	77	237	293	6.29e-12	59.7
MS.gene80863.t1	MTR_2g033000	55.814	43	19	0	35	77	256	298	2.78e-11	58.2

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene80863.t1	AT4G31860	63.333	60	22	18	77	232	291	2.02e-26	98.6
MS.gene80863.t1	AT2G25070	65.000	60	21	18	77	232	291	3.73e-26	99.0
MS.gene80863.t1	AT2G25070	65.000	60	21	18	77	232	291	3.73e-26	99.0
MS.gene80863.t1	AT4G31860	63.333	60	22	18	77	232	291	5.39e-26	98.6
MS.gene80863.t1	AT4G31860	63.636	44	16	18	61	232	275	3.75e-17	73.6

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Find 15 sgRNAs with CRISPR-Local

Find 80 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTACAATGTTTATGTC+TGG	0.418978	7.4:+69648310	None:intergenic
AATGTTTATGTCTGGATTGG+CGG	0.464448	7.4:+69648318	None:intergenic
AGCGAATGAGCCTGTGTTAT+TGG	0.325490	7.4:-69649476	MS.gene80863:CDS
ATTCGCTACACCTACAACCA+TGG	0.597019	7.4:+69649492	None:intergenic
CTACCAATTGTTGGCTTGAT+AGG	0.213993	7.4:+69648003	None:intergenic
GTAAGTAGCTCCAATAACAC+AGG	0.642874	7.4:+69649466	None:intergenic
GTACAAAATCTACCAATTGT+TGG	0.434248	7.4:+69647994	None:intergenic
TAAACTCATCTTCGTCATGA+AGG	0.558190	7.4:+69648139	None:intergenic
TACAATGTTTATGTCTGGAT+TGG	0.435988	7.4:+69648315	None:intergenic
TGATAGCTTGTGATGGCATT+TGG	0.432620	7.4:-69648113	MS.gene80863:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATACTTGTATGCATGTTGTT+TGG	-	chr7.4:69648771-69648790	MS.gene80863:intron	30.0%
CAATAATTGCTTAGCTACAA+GGG	-	chr7.4:69648315-69648334	MS.gene80863:intron	30.0%
GTACAAAATCTACCAATTGT+TGG	+	chr7.4:69649302-69649321	None:intergenic	30.0%
GTCATTAGATGATAGAATGA+CGG	-	chr7.4:69648362-69648381	MS.gene80863:CDS	30.0%
TACAATGTTTATGTCTGGAT+TGG	+	chr7.4:69648981-69649000	None:intergenic	30.0%
TATTGTAGCATGCGTATAAA+AGG	-	chr7.4:69648696-69648715	MS.gene80863:intron	30.0%
TTCATATACAAGACAGTACT+AGG	+	chr7.4:69649431-69649450	None:intergenic	30.0%
TTGCACAAACTAGATATGAT+AGG	+	chr7.4:69647985-69648004	None:intergenic	30.0%
TTTCAACATCATGGTCTAAT+AGG	-	chr7.4:69648534-69648553	MS.gene80863:intron	30.0%
ACATTAACCATGACCTTTAG+TGG	+	chr7.4:69647896-69647915	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	69647803	69649512	69647803	ID=MS.gene80863
chr7.4	mRNA	69647803	69649512	69647803	ID=MS.gene80863.t1;Parent=MS.gene80863
chr7.4	exon	69649461	69649512	69649461	ID=MS.gene80863.t1.exon1;Parent=MS.gene80863.t1
chr7.4	CDS	69649461	69649512	69649461	ID=cds.MS.gene80863.t1;Parent=MS.gene80863.t1
chr7.4	exon	69648318	69648394	69648318	ID=MS.gene80863.t1.exon2;Parent=MS.gene80863.t1
chr7.4	CDS	69648318	69648394	69648318	ID=cds.MS.gene80863.t1;Parent=MS.gene80863.t1
chr7.4	exon	69648114	69648166	69648114	ID=MS.gene80863.t1.exon3;Parent=MS.gene80863.t1
chr7.4	CDS	69648114	69648166	69648114	ID=cds.MS.gene80863.t1;Parent=MS.gene80863.t1
chr7.4	exon	69647977	69648031	69647977	ID=MS.gene80863.t1.exon4;Parent=MS.gene80863.t1
chr7.4	CDS	69647977	69648031	69647977	ID=cds.MS.gene80863.t1;Parent=MS.gene80863.t1
chr7.4	exon	69647803	69647832	69647803	ID=MS.gene80863.t1.exon5;Parent=MS.gene80863.t1
chr7.4	CDS	69647803	69647832	69647803	ID=cds.MS.gene80863.t1;Parent=MS.gene80863.t1

Gene Sequence

>MS.gene80863

ATGGTTGTAGGTGTAGCGAATGAGCCTGTGTTATTGGAGCTACTTACTTCAGGTTGGTCATAAAAGTTCTACGTTTGATTCAATTTTAGGCCACTAAAGGTCATGGTTAATGTTGTTTTCCTTTCAATGTTGTTGTTTTACATGAAGATGGTGTGTTTGATGAAGTGATTCATAGTAATCCTATCATATCTAGTTTGTGCAAACTTGATTGTGCCCAAAGTAGATTTCTATATTTTCCTTCCTCTATGACTTTATAATAATAGAGTTTGTTCTTTTATTTACTTGCTTTGTAGGGAGTCACTCATGTGACTTGATTCACAAGAACAGCTCTTTTTCCTTCGAGGTAGGATGATGAAAAGTTACCAAGAAGAGTGCGTAAGAATCACATTATGCATATATGTTGCTTTGTGTTTACGCCGTAACCCTTTAATTTGTTTTTGTTAATTTTGTTGGGTTGCCGGTACAGGATCTGTTTTTGCTTAGTTTGTTTCTTCTTACTAAGGTATTTCAACCAATAATTGCTTAGCTACAAGGGTGCTGAGTTTGAAATTGTTGACGCGTCATTAGATGATAGAATGACGGTATGGCCGACTAATTTCTTTGAAAATATTGATGGCAACTCTTTACTTGTTCTCTCTATAGATAATTAAATGAAGAGAAAAGATTTAATTGCCTTGCTTTATTAATAGTAACTACCATTACTTGTGTATTAAGCTATTGTTATAATAGAATTTCAACATCATGGTCTAATAGGTAGTTCAGATCTATATTTTGTTTTACTCATTCATTTGTCTTTTGAATTCAGTATTCTCATCTAGTAATGTATGTTCTTATCAATTTTCTTTTGAATTCTGTATGCTTGTCTGATTATGTTTTTTTTTTTTTCTTTCAAATATTGTAGCATGCGTATAAAAGGAACCTTCCTATTATTTTATTTTGTATGCTTTATTTTGGAGGCAAGAGGAGGTATACTTGTATGCATGTTGTTTGGTTTCAAATTGCATAATTGTAGATTTCAAGATATAAAACTGTTTCTTCTTGACTATAATTTCTTTGCGAAGAAATTGCACCTATATGGTTTATATTGTTTCCATTTAATATGTTTTAATATTCTGCAGGTGACGTGGACTTCAAAAATAATAGATTTCTTTCTGCTGAAAAACAAGTTGTGACCGCCAATCCAGACATAAACATTGTAAGTTTGCTCCATTGCAAGTCAGATTACCCTATTTCTCAATAGACAATATGGTTGAATCAATAATGAAATGCTTAGTCAAGAGTTTCGATATTTGCTACTACTACATGTGTTTCTGAATGAGAGTTTTGTTTTTGGAATTATATCATAGGTTGACCTTCATGACGAAGATGAGTTTATAGTGATAGCTTGTGATGGCATTTGGTATGGTTCTTATGCTTGATATCCACAATTCCCCATTTTAATTAAATGTCATTTCTTGAAAATTCTTTTTCTTACGTTTCAGGGATTGCCTATCAAGCCAACAATTGGTAGATTTTGTACGTCAAGAACTTCTCTTGGTGAGTGTTCAAGTTATACATTTTTTTTTTCAATCAGATTGTTTGAGTATTCAATACTCAATTATTCATGATATTTAATACAGCACTTACCTAGTACTGTCTTGTATATGAAATGTTATTTGGACTTACATAATTTGGTTGTAGAGAATTCGCAAAGCATGCACAAACTTATGA

Protein sequence

>MS.gene80863.t1

MVVGVANEPVLLELLTSGDVDFKNNRFLSAEKQVVTANPDINIVDLHDEDEFIVIACDGIWDCLSSQQLVDFVRQELLLRIRKACTNL