Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80863.t1 | XP_024631053.1 | 95.4 | 65 | 3 | 0 | 18 | 82 | 232 | 296 | 8.10E-27 | 129.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80863.t1 | Q6ETK3 | 66.7 | 60 | 20 | 0 | 18 | 77 | 232 | 291 | 3.7e-20 | 98.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80863.t1 | G7I891 | 95.4 | 65 | 3 | 0 | 18 | 82 | 232 | 296 | 5.9e-27 | 129.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056174 | MS.gene80863 | 0.803212 | 3.88E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80863.t1 | MTR_1g014190 | 95.385 | 65 | 3 | 0 | 18 | 82 | 232 | 296 | 1.36e-38 | 131 |
| MS.gene80863.t1 | MTR_1g014190 | 95.385 | 65 | 3 | 0 | 18 | 82 | 270 | 334 | 3.74e-38 | 131 |
| MS.gene80863.t1 | MTR_3g105880 | 76.667 | 60 | 14 | 0 | 18 | 77 | 232 | 291 | 2.83e-29 | 106 |
| MS.gene80863.t1 | MTR_3g105880 | 72.308 | 65 | 18 | 0 | 18 | 82 | 232 | 296 | 7.32e-29 | 105 |
| MS.gene80863.t1 | MTR_4g007440 | 66.667 | 60 | 20 | 0 | 18 | 77 | 232 | 291 | 3.55e-25 | 96.3 |
| MS.gene80863.t1 | MTR_4g123080 | 50.000 | 60 | 27 | 1 | 18 | 77 | 142 | 198 | 2.77e-12 | 60.5 |
| MS.gene80863.t1 | MTR_4g123080 | 50.000 | 60 | 27 | 1 | 18 | 77 | 255 | 311 | 3.56e-12 | 60.5 |
| MS.gene80863.t1 | MTR_4g123080 | 50.000 | 60 | 27 | 1 | 18 | 77 | 237 | 293 | 6.29e-12 | 59.7 |
| MS.gene80863.t1 | MTR_2g033000 | 55.814 | 43 | 19 | 0 | 35 | 77 | 256 | 298 | 2.78e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80863.t1 | AT4G31860 | 63.333 | 60 | 22 | 0 | 18 | 77 | 232 | 291 | 2.02e-26 | 98.6 |
| MS.gene80863.t1 | AT2G25070 | 65.000 | 60 | 21 | 0 | 18 | 77 | 232 | 291 | 3.73e-26 | 99.0 |
| MS.gene80863.t1 | AT2G25070 | 65.000 | 60 | 21 | 0 | 18 | 77 | 232 | 291 | 3.73e-26 | 99.0 |
| MS.gene80863.t1 | AT4G31860 | 63.333 | 60 | 22 | 0 | 18 | 77 | 232 | 291 | 5.39e-26 | 98.6 |
| MS.gene80863.t1 | AT4G31860 | 63.636 | 44 | 16 | 0 | 18 | 61 | 232 | 275 | 3.75e-17 | 73.6 |
Find 15 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACCAATTGTTGGCTTGAT+AGG | 0.213993 | 7.4:+69648003 | None:intergenic |
| AGCGAATGAGCCTGTGTTAT+TGG | 0.325490 | 7.4:-69649476 | MS.gene80863:CDS |
| TTATTGGAGCTACTTACTTC+AGG | 0.327624 | 7.4:-69649460 | MS.gene80863:intron |
| AAACTTACAATGTTTATGTC+TGG | 0.418978 | 7.4:+69648310 | None:intergenic |
| TGATAGCTTGTGATGGCATT+TGG | 0.432620 | 7.4:-69648113 | MS.gene80863:intron |
| GTACAAAATCTACCAATTGT+TGG | 0.434248 | 7.4:+69647994 | None:intergenic |
| TACAATGTTTATGTCTGGAT+TGG | 0.435988 | 7.4:+69648315 | None:intergenic |
| AATGTTTATGTCTGGATTGG+CGG | 0.464448 | 7.4:+69648318 | None:intergenic |
| TTGCCTATCAAGCCAACAAT+TGG | 0.471715 | 7.4:-69648006 | MS.gene80863:CDS |
| TGTACGTCAAGAACTTCTCT+TGG | 0.476519 | 7.4:-69647976 | MS.gene80863:intron |
| TAAACTCATCTTCGTCATGA+AGG | 0.558190 | 7.4:+69648139 | None:intergenic |
| TTAATATTCTGCAGGTGACG+TGG | 0.584418 | 7.4:-69648386 | MS.gene80863:intron |
| ATTCGCTACACCTACAACCA+TGG | 0.597019 | 7.4:+69649492 | None:intergenic |
| GTAAGTAGCTCCAATAACAC+AGG | 0.642874 | 7.4:+69649466 | None:intergenic |
| TTTATAGTGATAGCTTGTGA+TGG | 0.644189 | 7.4:-69648120 | MS.gene80863:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATGACATTTAATTAAAAT+GGG | + | chr7.4:69649236-69649255 | None:intergenic | 10.0% |
| !! | AATTAACAAAAACAAATTAA+AGG | + | chr7.4:69648229-69648248 | None:intergenic | 10.0% |
| !! | ATTAACAAAAACAAATTAAA+GGG | + | chr7.4:69648228-69648247 | None:intergenic | 10.0% |
| !! | GAATATTAAAACATATTAAA+TGG | + | chr7.4:69648896-69648915 | None:intergenic | 10.0% |
| !! | AAAATATAGAAATCTACTTT+GGG | + | chr7.4:69648019-69648038 | None:intergenic | 15.0% |
| !! | AATGACATTTAATTAAAATG+GGG | + | chr7.4:69649235-69649254 | None:intergenic | 15.0% |
| !! | AGCATACAAAATAAAATAAT+AGG | + | chr7.4:69648728-69648747 | None:intergenic | 15.0% |
| !! | GAAATGACATTTAATTAAAA+TGG | + | chr7.4:69649237-69649256 | None:intergenic | 15.0% |
| !! | TACAAAATAAAATAATAGGA+AGG | + | chr7.4:69648724-69648743 | None:intergenic | 15.0% |
| !!! | AATTTCTTTGAAAATATTGA+TGG | - | chr7.4:69648396-69648415 | MS.gene80863:intron | 15.0% |
| !!! | ATATTTTCAAAGAAATTAGT+CGG | + | chr7.4:69648393-69648412 | None:intergenic | 15.0% |
| !!! | TTATTTTGTATGCTTTATTT+TGG | - | chr7.4:69648734-69648753 | MS.gene80863:intron | 15.0% |
| !!! | TTGTTTTTGTTAATTTTGTT+GGG | - | chr7.4:69648234-69648253 | MS.gene80863:intron | 15.0% |
| !!! | TTTGTTTTTGTTAATTTTGT+TGG | - | chr7.4:69648233-69648252 | MS.gene80863:intron | 15.0% |
| !! | AAAAACAAATTAAAGGGTTA+CGG | + | chr7.4:69648222-69648241 | None:intergenic | 20.0% |
| !! | ATAATAGAATTTCAACATCA+TGG | - | chr7.4:69648525-69648544 | MS.gene80863:intron | 20.0% |
| !! | ATATTGTCTATTGAGAAATA+GGG | + | chr7.4:69649030-69649049 | None:intergenic | 20.0% |
| !! | CTATTATTATAAAGTCATAG+AGG | + | chr7.4:69648046-69648065 | None:intergenic | 20.0% |
| !! | CTTGTATATGAAATGTTATT+TGG | - | chr7.4:69649439-69649458 | MS.gene80863:intron | 20.0% |
| !! | GAAAATATAGAAATCTACTT+TGG | + | chr7.4:69648020-69648039 | None:intergenic | 20.0% |
| !! | TAGTTACTATTAATAAAGCA+AGG | + | chr7.4:69648478-69648497 | None:intergenic | 20.0% |
| !! | TTATTTGGACTTACATAATT+TGG | - | chr7.4:69649454-69649473 | MS.gene80863:intron | 20.0% |
| !!! | AATATGTTTTAATATTCTGC+AGG | - | chr7.4:69648899-69648918 | MS.gene80863:intron | 20.0% |
| !!! | TGTTTTTGGAATTATATCAT+AGG | - | chr7.4:69649127-69649146 | MS.gene80863:intron | 20.0% |
| !!! | TTTTATTTACTTGCTTTGTA+GGG | - | chr7.4:69648075-69648094 | MS.gene80863:intron | 20.0% |
| ! | AAACTTACAATGTTTATGTC+TGG | + | chr7.4:69648986-69649005 | None:intergenic | 25.0% |
| ! | ATAGCTTAATACACAAGTAA+TGG | + | chr7.4:69648501-69648520 | None:intergenic | 25.0% |
| ! | CATATTGTCTATTGAGAAAT+AGG | + | chr7.4:69649031-69649050 | None:intergenic | 25.0% |
| ! | GGAAACAATATAAACCATAT+AGG | + | chr7.4:69648875-69648894 | None:intergenic | 25.0% |
| ! | TATTATAAAGTCATAGAGGA+AGG | + | chr7.4:69648042-69648061 | None:intergenic | 25.0% |
| ! | TGTAAAACAACAACATTGAA+AGG | + | chr7.4:69647925-69647944 | None:intergenic | 25.0% |
| ! | TTAATTAAAATGGGGAATTG+TGG | + | chr7.4:69649227-69649246 | None:intergenic | 25.0% |
| !! | AATTCTTTTTCTTACGTTTC+AGG | - | chr7.4:69649262-69649281 | MS.gene80863:intron | 25.0% |
| !! | ATTCTTTTTCTTACGTTTCA+GGG | - | chr7.4:69649263-69649282 | MS.gene80863:intron | 25.0% |
| !! | CTATTTCTCAATAGACAATA+TGG | - | chr7.4:69649029-69649048 | MS.gene80863:intron | 25.0% |
| !! | TCTACGTTTGATTCAATTTT+AGG | - | chr7.4:69647870-69647889 | MS.gene80863:intron | 25.0% |
| !!! | CTTTTATTTACTTGCTTTGT+AGG | - | chr7.4:69648074-69648093 | MS.gene80863:intron | 25.0% |
| !!! | TAGTTTGTTTCTTCTTACTA+AGG | - | chr7.4:69648283-69648302 | MS.gene80863:intron | 25.0% |
| !!! | TTTTGTATGCTTTATTTTGG+AGG | - | chr7.4:69648737-69648756 | MS.gene80863:intron | 25.0% |
| ATACTTGTATGCATGTTGTT+TGG | - | chr7.4:69648771-69648790 | MS.gene80863:intron | 30.0% | |
| CAATAATTGCTTAGCTACAA+GGG | - | chr7.4:69648315-69648334 | MS.gene80863:intron | 30.0% | |
| GTACAAAATCTACCAATTGT+TGG | + | chr7.4:69649302-69649321 | None:intergenic | 30.0% | |
| GTCATTAGATGATAGAATGA+CGG | - | chr7.4:69648362-69648381 | MS.gene80863:CDS | 30.0% | |
| TACAATGTTTATGTCTGGAT+TGG | + | chr7.4:69648981-69649000 | None:intergenic | 30.0% | |
| TATTGTAGCATGCGTATAAA+AGG | - | chr7.4:69648696-69648715 | MS.gene80863:intron | 30.0% | |
| TTCATATACAAGACAGTACT+AGG | + | chr7.4:69649431-69649450 | None:intergenic | 30.0% | |
| TTGCACAAACTAGATATGAT+AGG | + | chr7.4:69647985-69648004 | None:intergenic | 30.0% | |
| TTTCAACATCATGGTCTAAT+AGG | - | chr7.4:69648534-69648553 | MS.gene80863:intron | 30.0% | |
| ! | ATTCAATTTTAGGCCACTAA+AGG | - | chr7.4:69647880-69647899 | MS.gene80863:intron | 30.0% |
| ! | TTTATAGTGATAGCTTGTGA+TGG | - | chr7.4:69649173-69649192 | MS.gene80863:intron | 30.0% |
| !!! | CTGAATGAGAGTTTTGTTTT+TGG | - | chr7.4:69649113-69649132 | MS.gene80863:intron | 30.0% |
| !!! | GTTGTTGTTTTACATGAAGA+TGG | - | chr7.4:69647931-69647950 | MS.gene80863:intron | 30.0% |
| ACATTAACCATGACCTTTAG+TGG | + | chr7.4:69647896-69647915 | None:intergenic | 35.0% | |
| CCAATAATTGCTTAGCTACA+AGG | - | chr7.4:69648314-69648333 | MS.gene80863:intron | 35.0% | |
| CCTTGTAGCTAAGCAATTAT+TGG | + | chr7.4:69648317-69648336 | None:intergenic | 35.0% | |
| TAAACTCATCTTCGTCATGA+AGG | + | chr7.4:69649157-69649176 | None:intergenic | 35.0% | |
| TAGATGATAGAATGACGGTA+TGG | - | chr7.4:69648367-69648386 | MS.gene80863:CDS | 35.0% | |
| TTATTGGAGCTACTTACTTC+AGG | - | chr7.4:69647833-69647852 | MS.gene80863:intron | 35.0% | |
| ! | AATGTTTATGTCTGGATTGG+CGG | + | chr7.4:69648978-69648997 | None:intergenic | 35.0% |
| !! | TGTTAATTTTGTTGGGTTGC+CGG | - | chr7.4:69648241-69648260 | MS.gene80863:intron | 35.0% |
| AGCAAAAACAGATCCTGTAC+CGG | + | chr7.4:69648263-69648282 | None:intergenic | 40.0% | |
| GCGAAGAAATTGCACCTATA+TGG | - | chr7.4:69648858-69648877 | MS.gene80863:intron | 40.0% | |
| GTAAGTAGCTCCAATAACAC+AGG | + | chr7.4:69647830-69647849 | None:intergenic | 40.0% | |
| TGATTCTTACGCACTCTTCT+TGG | + | chr7.4:69648168-69648187 | None:intergenic | 40.0% | |
| TGTACGTCAAGAACTTCTCT+TGG | - | chr7.4:69649317-69649336 | MS.gene80863:intron | 40.0% | |
| TTAATATTCTGCAGGTGACG+TGG | - | chr7.4:69648907-69648926 | MS.gene80863:intron | 40.0% | |
| TTGCCTATCAAGCCAACAAT+TGG | - | chr7.4:69649287-69649306 | MS.gene80863:intron | 40.0% | |
| ! | TAGGGTAATCTGACTTGCAA+TGG | + | chr7.4:69649012-69649031 | None:intergenic | 40.0% |
| ! | TGATAGCTTGTGATGGCATT+TGG | - | chr7.4:69649180-69649199 | MS.gene80863:intron | 40.0% |
| ! | TTTTAGGCCACTAAAGGTCA+TGG | - | chr7.4:69647886-69647905 | MS.gene80863:intron | 40.0% |
| ! | TTTTCATCATCCTACCTCGA+AGG | + | chr7.4:69648141-69648160 | None:intergenic | 40.0% |
| !! | CTACCAATTGTTGGCTTGAT+AGG | + | chr7.4:69649293-69649312 | None:intergenic | 40.0% |
| !!! | TGCTTTATTTTGGAGGCAAG+AGG | - | chr7.4:69648744-69648763 | MS.gene80863:intron | 40.0% |
| !!! | TTTATTTTGGAGGCAAGAGG+AGG | - | chr7.4:69648747-69648766 | MS.gene80863:intron | 40.0% |
| AGCGAATGAGCCTGTGTTAT+TGG | - | chr7.4:69647817-69647836 | MS.gene80863:intron | 45.0% | |
| GCTTGTGATGGCATTTGGTA+TGG | - | chr7.4:69649185-69649204 | MS.gene80863:intron | 45.0% | |
| TGGAGCTACTTACTTCAGGT+TGG | - | chr7.4:69647837-69647856 | MS.gene80863:intron | 45.0% | |
| ! | AGCTCTTTTTCCTTCGAGGT+AGG | - | chr7.4:69648128-69648147 | MS.gene80863:CDS | 45.0% |
| ! | GAACAGCTCTTTTTCCTTCG+AGG | - | chr7.4:69648124-69648143 | MS.gene80863:CDS | 45.0% |
| !! | TTTTGTTGGGTTGCCGGTAC+AGG | - | chr7.4:69648247-69648266 | MS.gene80863:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 69647803 | 69649512 | 69647803 | ID=MS.gene80863 |
| chr7.4 | mRNA | 69647803 | 69649512 | 69647803 | ID=MS.gene80863.t1;Parent=MS.gene80863 |
| chr7.4 | exon | 69649461 | 69649512 | 69649461 | ID=MS.gene80863.t1.exon1;Parent=MS.gene80863.t1 |
| chr7.4 | CDS | 69649461 | 69649512 | 69649461 | ID=cds.MS.gene80863.t1;Parent=MS.gene80863.t1 |
| chr7.4 | exon | 69648318 | 69648394 | 69648318 | ID=MS.gene80863.t1.exon2;Parent=MS.gene80863.t1 |
| chr7.4 | CDS | 69648318 | 69648394 | 69648318 | ID=cds.MS.gene80863.t1;Parent=MS.gene80863.t1 |
| chr7.4 | exon | 69648114 | 69648166 | 69648114 | ID=MS.gene80863.t1.exon3;Parent=MS.gene80863.t1 |
| chr7.4 | CDS | 69648114 | 69648166 | 69648114 | ID=cds.MS.gene80863.t1;Parent=MS.gene80863.t1 |
| chr7.4 | exon | 69647977 | 69648031 | 69647977 | ID=MS.gene80863.t1.exon4;Parent=MS.gene80863.t1 |
| chr7.4 | CDS | 69647977 | 69648031 | 69647977 | ID=cds.MS.gene80863.t1;Parent=MS.gene80863.t1 |
| chr7.4 | exon | 69647803 | 69647832 | 69647803 | ID=MS.gene80863.t1.exon5;Parent=MS.gene80863.t1 |
| chr7.4 | CDS | 69647803 | 69647832 | 69647803 | ID=cds.MS.gene80863.t1;Parent=MS.gene80863.t1 |
| Gene Sequence |
| Protein sequence |
