Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80903.t1 | RHN44589.1 | 76.6 | 111 | 22 | 2 | 1 | 111 | 181 | 287 | 2.90E-37 | 164.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80903.t1 | A0A396GTV9 | 76.6 | 111 | 22 | 2 | 1 | 111 | 181 | 287 | 2.1e-37 | 164.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05415 | MS.gene80903 | 0.800208 | 1.61E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCATGTCCTGGCATAAAA+AGG | 0.316820 | 7.4:+81206649 | MS.gene80903:CDS |
| GATAGCGAGAACCCTTGTTT+TGG | 0.330405 | 7.4:+81206693 | MS.gene80903:CDS |
| AGTTCTGCAACTTCAAAATC+TGG | 0.338634 | 7.4:+81206428 | MS.gene80903:CDS |
| CAACTTCAACGAAGTCAACT+TGG | 0.413353 | 7.4:-81206541 | None:intergenic |
| GACTGAAGACCGTGTGCAAA+GGG | 0.471682 | 7.4:+81206577 | MS.gene80903:CDS |
| CAACTTGGGAAGTGTAATGA+TGG | 0.511568 | 7.4:-81206526 | None:intergenic |
| CTATCTATGAGCTTTACTGT+AGG | 0.513821 | 7.4:-81206675 | None:intergenic |
| GGCGGCTCATTATCATGTCC+TGG | 0.535843 | 7.4:+81206639 | MS.gene80903:CDS |
| CGACTGAAGACCGTGTGCAA+AGG | 0.549879 | 7.4:+81206576 | MS.gene80903:CDS |
| TATCTATGAGCTTTACTGTA+GGG | 0.549901 | 7.4:-81206674 | None:intergenic |
| TTGCACACGGTCTTCAGTCG+TGG | 0.570525 | 7.4:-81206573 | None:intergenic |
| TGAAGTTGCAGAACTAATTG+TGG | 0.600660 | 7.4:-81206420 | None:intergenic |
| AACTTCAACGAAGTCAACTT+GGG | 0.600789 | 7.4:-81206540 | None:intergenic |
| CAACCGAGTTTGTTCATCGG+CGG | 0.603904 | 7.4:+81206621 | MS.gene80903:CDS |
| TGCAAAGGGATTATACGTTG+TGG | 0.604247 | 7.4:+81206591 | MS.gene80903:CDS |
| TCACAACCGAGTTTGTTCAT+CGG | 0.615829 | 7.4:+81206618 | MS.gene80903:CDS |
| ACGTATAATCCCTTTGCACA+CGG | 0.633230 | 7.4:-81206586 | None:intergenic |
| GAGCCGCCGATGAACAAACT+CGG | 0.689153 | 7.4:-81206624 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGGAAAAATCCCAAAACAA+GGG | - | chr7.4:81206707-81206726 | None:intergenic | 30.0% | |
| TAAGGAAAAATCCCAAAACA+AGG | - | chr7.4:81206708-81206727 | None:intergenic | 30.0% | |
| TATCTATGAGCTTTACTGTA+GGG | - | chr7.4:81206677-81206696 | None:intergenic | 30.0% | |
| AACTTCAACGAAGTCAACTT+GGG | - | chr7.4:81206543-81206562 | None:intergenic | 35.0% | |
| AGTTCTGCAACTTCAAAATC+TGG | + | chr7.4:81206428-81206447 | MS.gene80903:CDS | 35.0% | |
| CTATCTATGAGCTTTACTGT+AGG | - | chr7.4:81206678-81206697 | None:intergenic | 35.0% | |
| TATCATGTCCTGGCATAAAA+AGG | + | chr7.4:81206649-81206668 | MS.gene80903:CDS | 35.0% | |
| TGAAGTTGCAGAACTAATTG+TGG | - | chr7.4:81206423-81206442 | None:intergenic | 35.0% | |
| ACGTATAATCCCTTTGCACA+CGG | - | chr7.4:81206589-81206608 | None:intergenic | 40.0% | |
| CAACTTCAACGAAGTCAACT+TGG | - | chr7.4:81206544-81206563 | None:intergenic | 40.0% | |
| TGCAAAGGGATTATACGTTG+TGG | + | chr7.4:81206591-81206610 | MS.gene80903:CDS | 40.0% | |
| ! | CAACTTGGGAAGTGTAATGA+TGG | - | chr7.4:81206529-81206548 | None:intergenic | 40.0% |
| ! | TCACAACCGAGTTTGTTCAT+CGG | + | chr7.4:81206618-81206637 | MS.gene80903:CDS | 40.0% |
| !!! | ATAGCGAGAACCCTTGTTTT+GGG | + | chr7.4:81206694-81206713 | MS.gene80903:CDS | 40.0% |
| !! | GATAGCGAGAACCCTTGTTT+TGG | + | chr7.4:81206693-81206712 | MS.gene80903:CDS | 45.0% |
| GACTGAAGACCGTGTGCAAA+GGG | + | chr7.4:81206577-81206596 | MS.gene80903:CDS | 50.0% | |
| ! | CAACCGAGTTTGTTCATCGG+CGG | + | chr7.4:81206621-81206640 | MS.gene80903:CDS | 50.0% |
| !! | GTAGGGTGCCTTTTTATGCC+AGG | - | chr7.4:81206660-81206679 | None:intergenic | 50.0% |
| CGACTGAAGACCGTGTGCAA+AGG | + | chr7.4:81206576-81206595 | MS.gene80903:CDS | 55.0% | |
| GAGCCGCCGATGAACAAACT+CGG | - | chr7.4:81206627-81206646 | None:intergenic | 55.0% | |
| GGCGGCTCATTATCATGTCC+TGG | + | chr7.4:81206639-81206658 | MS.gene80903:CDS | 55.0% | |
| TTGCACACGGTCTTCAGTCG+TGG | - | chr7.4:81206576-81206595 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 81206392 | 81206728 | 81206392 | ID=MS.gene80903 |
| chr7.4 | mRNA | 81206392 | 81206728 | 81206392 | ID=MS.gene80903.t1;Parent=MS.gene80903 |
| chr7.4 | exon | 81206392 | 81206728 | 81206392 | ID=MS.gene80903.t1.exon1;Parent=MS.gene80903.t1 |
| chr7.4 | CDS | 81206392 | 81206728 | 81206392 | ID=cds.MS.gene80903.t1;Parent=MS.gene80903.t1 |
| Gene Sequence |
| Protein sequence |
