Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80959.t1 | XP_003610296.2 | 86.9 | 153 | 17 | 2 | 1 | 150 | 38 | 190 | 1.90E-66 | 261.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80959.t1 | G7JFL0 | 86.9 | 153 | 17 | 2 | 1 | 150 | 1 | 153 | 1.3e-66 | 261.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056677 | MS.gene80959 | 0.802554 | 5.31E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80959.t1 | MTR_4g130640 | 86.928 | 153 | 17 | 2 | 1 | 150 | 1 | 153 | 5.53e-92 | 264 |
| MS.gene80959.t1 | MTR_7g069410 | 45.033 | 151 | 41 | 5 | 1 | 150 | 1 | 110 | 6.04e-28 | 100 |
| MS.gene80959.t1 | MTR_6g023775 | 41.237 | 97 | 23 | 2 | 80 | 142 | 65 | 161 | 2.00e-13 | 64.3 |
| MS.gene80959.t1 | MTR_7g031710 | 70.270 | 37 | 11 | 0 | 113 | 149 | 42 | 78 | 4.18e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 31 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCTTTGAAAAGAAATAAAA+TGG | 0.207796 | 7.3:+90737512 | MS.gene80959:CDS |
| GAAATAAAATGGATCGAAAA+AGG | 0.213477 | 7.3:+90737523 | MS.gene80959:CDS |
| CATCATGTTCTGAGAGAAAT+TGG | 0.219553 | 7.3:+90737469 | MS.gene80959:CDS |
| CTAGTAACACTTTCTTCTAT+TGG | 0.245408 | 7.3:-90737249 | None:intergenic |
| TAGTGGTTTACTATCATCTT+GGG | 0.287206 | 7.3:-90737292 | None:intergenic |
| TTTCACTTATTGAAAATTGC+TGG | 0.358067 | 7.3:+90737423 | MS.gene80959:CDS |
| AATTGCTGGTGAAGGAGTTC+AGG | 0.416502 | 7.3:+90737437 | MS.gene80959:CDS |
| TGAGTTATCTTTAGATGAAC+CGG | 0.418024 | 7.3:+90737599 | MS.gene80959:CDS |
| GCTACAATGTTCTTTGATGA+TGG | 0.418641 | 7.3:+90737630 | MS.gene80959:CDS |
| CTGCAGGTGGAAACAAGCTT+TGG | 0.435890 | 7.3:+90737349 | MS.gene80959:CDS |
| TAGAAGAAAGTGTTACTAGT+TGG | 0.440821 | 7.3:+90737253 | MS.gene80959:CDS |
| AACATTGTAGCCTCCAACTC+CGG | 0.456994 | 7.3:-90737618 | None:intergenic |
| ATAGTGGTTTACTATCATCT+TGG | 0.457884 | 7.3:-90737293 | None:intergenic |
| ATCTTTAGATGAACCGGAGT+TGG | 0.478188 | 7.3:+90737605 | MS.gene80959:CDS |
| GTAACACTTTCTTCTATTGG+AGG | 0.479958 | 7.3:-90737246 | None:intergenic |
| AAGATGATAGTAAACCACTA+TGG | 0.482539 | 7.3:+90737295 | MS.gene80959:CDS |
| TTTAGATGAACCGGAGTTGG+AGG | 0.500804 | 7.3:+90737608 | MS.gene80959:CDS |
| ATGTTCTTTGATGATGGAAA+CGG | 0.513119 | 7.3:+90737636 | MS.gene80959:CDS |
| GTGCATACTAATGTTGGAGT+TGG | 0.524300 | 7.3:+90737564 | MS.gene80959:CDS |
| GTTTATGTGCATACTAATGT+TGG | 0.532305 | 7.3:+90737558 | MS.gene80959:CDS |
| TCCTCTGAAGTAGTGAATGT+TGG | 0.537803 | 7.3:+90737687 | MS.gene80959:CDS |
| AGTGGTTTACTATCATCTTG+GGG | 0.567164 | 7.3:-90737291 | None:intergenic |
| AGAGGAAACACGGATTGTAT+CGG | 0.567785 | 7.3:-90737670 | None:intergenic |
| TTATTGAAAATTGCTGGTGA+AGG | 0.583272 | 7.3:+90737429 | MS.gene80959:CDS |
| TTCTTTGATGATGGAAACGG+AGG | 0.597380 | 7.3:+90737639 | MS.gene80959:CDS |
| GTGCAGAATAATGATTCTGC+AGG | 0.611185 | 7.3:+90737333 | MS.gene80959:CDS |
| CAGAATAATGATTCTGCAGG+TGG | 0.615421 | 7.3:+90737336 | MS.gene80959:CDS |
| TGATGATGGAAACGGAGGAG+AGG | 0.621097 | 7.3:+90737644 | MS.gene80959:CDS |
| TCCAACATTCACTACTTCAG+AGG | 0.626874 | 7.3:-90737688 | None:intergenic |
| TCACTACTTCAGAGGAAACA+CGG | 0.630269 | 7.3:-90737680 | None:intergenic |
| ACAGTGACAAATCTCCATAG+TGG | 0.653867 | 7.3:-90737309 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATCTTTGAAAAGAAATAAAA+TGG | + | chr7.3:90737512-90737531 | MS.gene80959:CDS | 15.0% |
| !! | AAATAAAATGGATCGAAAAA+GGG | + | chr7.3:90737524-90737543 | MS.gene80959:CDS | 20.0% |
| ! | GAAATAAAATGGATCGAAAA+AGG | + | chr7.3:90737523-90737542 | MS.gene80959:CDS | 25.0% |
| ! | TTTCACTTATTGAAAATTGC+TGG | + | chr7.3:90737423-90737442 | MS.gene80959:CDS | 25.0% |
| AAGATGATAGTAAACCACTA+TGG | + | chr7.3:90737295-90737314 | MS.gene80959:CDS | 30.0% | |
| ATAGTGGTTTACTATCATCT+TGG | - | chr7.3:90737296-90737315 | None:intergenic | 30.0% | |
| CTAGTAACACTTTCTTCTAT+TGG | - | chr7.3:90737252-90737271 | None:intergenic | 30.0% | |
| GTTTATGTGCATACTAATGT+TGG | + | chr7.3:90737558-90737577 | MS.gene80959:CDS | 30.0% | |
| TAGTGGTTTACTATCATCTT+GGG | - | chr7.3:90737295-90737314 | None:intergenic | 30.0% | |
| TGAGTTATCTTTAGATGAAC+CGG | + | chr7.3:90737599-90737618 | MS.gene80959:CDS | 30.0% | |
| TTATTGAAAATTGCTGGTGA+AGG | + | chr7.3:90737429-90737448 | MS.gene80959:CDS | 30.0% | |
| !! | ATGTTCTTTGATGATGGAAA+CGG | + | chr7.3:90737636-90737655 | MS.gene80959:CDS | 30.0% |
| !! | TAGAAGAAAGTGTTACTAGT+TGG | + | chr7.3:90737253-90737272 | MS.gene80959:CDS | 30.0% |
| AGTGGTTTACTATCATCTTG+GGG | - | chr7.3:90737294-90737313 | None:intergenic | 35.0% | |
| GCTACAATGTTCTTTGATGA+TGG | + | chr7.3:90737630-90737649 | MS.gene80959:CDS | 35.0% | |
| GTAACACTTTCTTCTATTGG+AGG | - | chr7.3:90737249-90737268 | None:intergenic | 35.0% | |
| ! | ACTTTTGTCAAAAAGAGGTG+AGG | + | chr7.3:90737379-90737398 | MS.gene80959:CDS | 35.0% |
| ! | GTGCAACTTTTGTCAAAAAG+AGG | + | chr7.3:90737374-90737393 | MS.gene80959:CDS | 35.0% |
| !! | CATCATGTTCTGAGAGAAAT+TGG | + | chr7.3:90737469-90737488 | MS.gene80959:CDS | 35.0% |
| ACAGTGACAAATCTCCATAG+TGG | - | chr7.3:90737312-90737331 | None:intergenic | 40.0% | |
| ATCTTTAGATGAACCGGAGT+TGG | + | chr7.3:90737605-90737624 | MS.gene80959:CDS | 40.0% | |
| CAGAATAATGATTCTGCAGG+TGG | + | chr7.3:90737336-90737355 | MS.gene80959:CDS | 40.0% | |
| GTGCAGAATAATGATTCTGC+AGG | + | chr7.3:90737333-90737352 | MS.gene80959:CDS | 40.0% | |
| GTGCATACTAATGTTGGAGT+TGG | + | chr7.3:90737564-90737583 | MS.gene80959:CDS | 40.0% | |
| TCACTACTTCAGAGGAAACA+CGG | - | chr7.3:90737683-90737702 | None:intergenic | 40.0% | |
| TCCAACATTCACTACTTCAG+AGG | - | chr7.3:90737691-90737710 | None:intergenic | 40.0% | |
| TCGAAAAAGGGCTGAAGATT+TGG | + | chr7.3:90737536-90737555 | MS.gene80959:CDS | 40.0% | |
| ! | AGAGGAAACACGGATTGTAT+CGG | - | chr7.3:90737673-90737692 | None:intergenic | 40.0% |
| !! | TCCTCTGAAGTAGTGAATGT+TGG | + | chr7.3:90737687-90737706 | MS.gene80959:CDS | 40.0% |
| !! | TTCTTTGATGATGGAAACGG+AGG | + | chr7.3:90737639-90737658 | MS.gene80959:CDS | 40.0% |
| AACATTGTAGCCTCCAACTC+CGG | - | chr7.3:90737621-90737640 | None:intergenic | 45.0% | |
| AATTGCTGGTGAAGGAGTTC+AGG | + | chr7.3:90737437-90737456 | MS.gene80959:CDS | 45.0% | |
| TTTAGATGAACCGGAGTTGG+AGG | + | chr7.3:90737608-90737627 | MS.gene80959:CDS | 45.0% | |
| CTGCAGGTGGAAACAAGCTT+TGG | + | chr7.3:90737349-90737368 | MS.gene80959:CDS | 50.0% | |
| !! | TGATGATGGAAACGGAGGAG+AGG | + | chr7.3:90737644-90737663 | MS.gene80959:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 90737243 | 90737716 | 90737243 | ID=MS.gene80959 |
| chr7.3 | mRNA | 90737243 | 90737716 | 90737243 | ID=MS.gene80959.t1;Parent=MS.gene80959 |
| chr7.3 | exon | 90737243 | 90737716 | 90737243 | ID=MS.gene80959.t1.exon1;Parent=MS.gene80959.t1 |
| chr7.3 | CDS | 90737243 | 90737716 | 90737243 | ID=cds.MS.gene80959.t1;Parent=MS.gene80959.t1 |
| Gene Sequence |
| Protein sequence |
