Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81124.t1 | XP_013464125.1 | 100 | 135 | 0 | 0 | 1 | 135 | 1 | 135 | 4.20E-67 | 263.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81124.t1 | Q9SL03 | 85.9 | 135 | 17 | 2 | 1 | 134 | 1 | 134 | 1.7e-56 | 219.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81124.t1 | A0A072VJ28 | 100.0 | 135 | 0 | 0 | 1 | 135 | 1 | 135 | 3.1e-67 | 263.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056302 | MS.gene81124 | 0.816278 | 6.01E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81124.t1 | MTR_2g061380 | 100.000 | 135 | 0 | 0 | 1 | 135 | 1 | 135 | 1.16e-88 | 281 |
| MS.gene81124.t1 | MTR_7g005950 | 84.252 | 127 | 18 | 2 | 9 | 134 | 13 | 138 | 5.34e-67 | 219 |
| MS.gene81124.t1 | MTR_7g005950 | 84.252 | 127 | 18 | 2 | 9 | 134 | 13 | 138 | 7.37e-67 | 219 |
| MS.gene81124.t1 | MTR_1g116370 | 87.097 | 124 | 14 | 2 | 12 | 134 | 19 | 141 | 3.03e-66 | 217 |
| MS.gene81124.t1 | MTR_7g027890 | 69.291 | 127 | 37 | 2 | 9 | 134 | 13 | 138 | 5.38e-56 | 172 |
| MS.gene81124.t1 | MTR_7g006330 | 69.421 | 121 | 31 | 4 | 19 | 134 | 2 | 121 | 4.09e-49 | 155 |
| MS.gene81124.t1 | MTR_3g096200 | 64.220 | 109 | 37 | 2 | 29 | 135 | 28 | 136 | 3.41e-40 | 142 |
| MS.gene81124.t1 | MTR_3g047390 | 55.462 | 119 | 51 | 1 | 9 | 125 | 15 | 133 | 3.23e-39 | 140 |
| MS.gene81124.t1 | MTR_8g093630 | 58.594 | 128 | 52 | 1 | 9 | 135 | 12 | 139 | 1.84e-37 | 135 |
| MS.gene81124.t1 | MTR_8g093630 | 65.741 | 108 | 36 | 1 | 29 | 135 | 32 | 139 | 1.50e-35 | 129 |
| MS.gene81124.t1 | MTR_1g101690 | 57.471 | 87 | 34 | 2 | 13 | 96 | 8 | 94 | 7.20e-28 | 99.0 |
| MS.gene81124.t1 | MTR_2g072160 | 49.524 | 105 | 50 | 3 | 30 | 133 | 63 | 165 | 9.92e-22 | 90.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81124.t1 | AT2G31960 | 85.926 | 135 | 17 | 2 | 1 | 134 | 1 | 134 | 5.16e-72 | 233 |
| MS.gene81124.t1 | AT2G31960 | 85.926 | 135 | 17 | 2 | 1 | 134 | 1 | 134 | 5.16e-72 | 233 |
| MS.gene81124.t1 | AT2G31960 | 85.926 | 135 | 17 | 2 | 1 | 134 | 1 | 134 | 5.16e-72 | 233 |
| MS.gene81124.t1 | AT2G31960 | 85.926 | 135 | 17 | 2 | 1 | 134 | 1 | 134 | 5.16e-72 | 233 |
| MS.gene81124.t1 | AT2G31960 | 85.926 | 135 | 17 | 2 | 1 | 134 | 1 | 134 | 6.02e-72 | 233 |
| MS.gene81124.t1 | AT1G05570 | 88.710 | 124 | 13 | 1 | 11 | 134 | 12 | 134 | 2.47e-69 | 226 |
| MS.gene81124.t1 | AT1G05570 | 88.710 | 124 | 13 | 1 | 11 | 134 | 12 | 134 | 2.47e-69 | 226 |
| MS.gene81124.t1 | AT1G05570 | 88.710 | 124 | 13 | 1 | 11 | 134 | 12 | 134 | 2.47e-69 | 226 |
| MS.gene81124.t1 | AT1G05570 | 88.710 | 124 | 13 | 1 | 11 | 134 | 12 | 134 | 2.83e-69 | 226 |
| MS.gene81124.t1 | AT5G13000 | 83.206 | 131 | 19 | 2 | 5 | 134 | 10 | 138 | 9.01e-67 | 218 |
| MS.gene81124.t1 | AT5G13000 | 83.206 | 131 | 19 | 2 | 5 | 134 | 10 | 138 | 1.00e-66 | 218 |
| MS.gene81124.t1 | AT2G13680 | 62.963 | 108 | 39 | 1 | 29 | 135 | 31 | 138 | 7.17e-40 | 142 |
| MS.gene81124.t1 | AT3G59100 | 68.041 | 97 | 31 | 0 | 30 | 126 | 38 | 134 | 9.59e-40 | 141 |
| MS.gene81124.t1 | AT1G06490 | 56.154 | 130 | 48 | 3 | 5 | 126 | 14 | 142 | 2.54e-36 | 131 |
| MS.gene81124.t1 | AT1G06490 | 56.154 | 130 | 48 | 3 | 5 | 126 | 14 | 142 | 5.68e-36 | 130 |
| MS.gene81124.t1 | AT5G36870 | 54.887 | 133 | 54 | 5 | 4 | 132 | 5 | 135 | 6.45e-33 | 122 |
| MS.gene81124.t1 | AT5G36870 | 54.887 | 133 | 54 | 5 | 4 | 132 | 5 | 135 | 6.45e-33 | 122 |
| MS.gene81124.t1 | AT5G36870 | 54.887 | 133 | 54 | 5 | 4 | 132 | 5 | 135 | 8.28e-33 | 121 |
| MS.gene81124.t1 | AT3G14570 | 47.967 | 123 | 57 | 5 | 8 | 125 | 27 | 147 | 3.71e-27 | 105 |
| MS.gene81124.t1 | AT3G14570 | 47.967 | 123 | 57 | 5 | 8 | 125 | 27 | 147 | 4.64e-27 | 105 |
| MS.gene81124.t1 | AT3G14570 | 47.967 | 123 | 57 | 5 | 8 | 125 | 27 | 147 | 4.64e-27 | 105 |
| MS.gene81124.t1 | AT5G36870 | 50.000 | 120 | 42 | 3 | 31 | 132 | 24 | 143 | 9.26e-26 | 101 |
| MS.gene81124.t1 | AT5G36870 | 50.000 | 120 | 42 | 3 | 31 | 132 | 24 | 143 | 9.26e-26 | 101 |
| MS.gene81124.t1 | AT3G14780 | 52.577 | 97 | 43 | 3 | 31 | 125 | 48 | 143 | 3.52e-25 | 98.2 |
Find 33 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTCAGACTGCCGGGAATTT+AGG | 0.286744 | 2.4:+31873327 | MS.gene81124:CDS |
| TTTAGGAGCAGACCCGATTC+TGG | 0.308679 | 2.4:+31873344 | MS.gene81124:CDS |
| TCAAGAAGATGTCTTATAGA+AGG | 0.344535 | 2.4:+31873268 | None:intergenic |
| GTAAAGCTTCAATATACTTT+CGG | 0.370645 | 2.4:-31874534 | None:intergenic |
| CGGGTCTGCTCCTAAATTCC+CGG | 0.389617 | 2.4:-31873337 | None:intergenic |
| CTCTTCTTCAACGTTTAGAA+AGG | 0.396766 | 2.4:+31874189 | MS.gene81124:CDS |
| TGAAGTCGTGCCATCTTCAT+TGG | 0.411667 | 2.4:+31873371 | MS.gene81124:CDS |
| TTTACAGAATGCTGCTGATA+AGG | 0.428474 | 2.4:+31874552 | MS.gene81124:CDS |
| ACGACTTCACTGTCCAGAAT+CGG | 0.440338 | 2.4:-31873357 | None:intergenic |
| CGACTTCACTGTCCAGAATC+GGG | 0.488590 | 2.4:-31873356 | None:intergenic |
| TCATTAGCAACACGGAGAAT+AGG | 0.492836 | 2.4:-31873405 | None:intergenic |
| TCTTCTTCAACGTTTAGAAA+GGG | 0.495453 | 2.4:+31874190 | MS.gene81124:CDS |
| GGAGAATGACACCACTTTGG+AGG | 0.508635 | 2.4:+31874459 | MS.gene81124:intron |
| CAAGAAGATGTCTTATAGAA+GGG | 0.510476 | 2.4:+31873269 | None:intergenic |
| AAGAAGATGTCTTATAGAAG+GGG | 0.531402 | 2.4:+31873270 | None:intergenic |
| GCAGGAGAATGACACCACTT+TGG | 0.533747 | 2.4:+31874456 | MS.gene81124:intron |
| TACTGCGGACTCAGACTGCC+GGG | 0.537339 | 2.4:+31873319 | MS.gene81124:CDS |
| ATACTGCGGACTCAGACTGC+CGG | 0.547922 | 2.4:+31873318 | MS.gene81124:CDS |
| AGACTAGATCCCACATCTAG+TGG | 0.552014 | 2.4:+31874140 | MS.gene81124:CDS |
| CTGAGCAGCAACCGCAGCGG+CGG | 0.553783 | 2.4:+31873295 | MS.gene81124:CDS |
| AACACCACGCCCACTAGATG+TGG | 0.554281 | 2.4:-31874150 | None:intergenic |
| GCGATCTCAACCAATGAAGA+TGG | 0.561569 | 2.4:-31873381 | None:intergenic |
| CTTTGCTCTTCCCTCCAAAG+TGG | 0.570097 | 2.4:-31874470 | None:intergenic |
| GAGAATGACACCACTTTGGA+GGG | 0.574104 | 2.4:+31874460 | MS.gene81124:CDS |
| GATCCCACATCTAGTGGGCG+TGG | 0.579829 | 2.4:+31874146 | MS.gene81124:CDS |
| AACCGCAGCGGCGGATACTG+CGG | 0.590591 | 2.4:+31873304 | MS.gene81124:CDS |
| AGAATGCTGCTGATAAGGAC+CGG | 0.600505 | 2.4:+31874557 | MS.gene81124:CDS |
| CTTCGACTTCATTAGCAACA+CGG | 0.601400 | 2.4:-31873413 | None:intergenic |
| GACTAGATCCCACATCTAGT+GGG | 0.630016 | 2.4:+31874141 | MS.gene81124:CDS |
| ACACCACGCCCACTAGATGT+GGG | 0.658359 | 2.4:-31874149 | None:intergenic |
| GTCCGCAGTATCCGCCGCTG+CGG | 0.670941 | 2.4:-31873306 | None:intergenic |
| AGAAGATGTCTTATAGAAGG+GGG | 0.678275 | 2.4:+31873271 | None:intergenic |
| GGTCTGAGCAGCAACCGCAG+CGG | 0.730372 | 2.4:+31873292 | MS.gene81124:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATTTAAATATAAATGCA+AGG | + | chr2.4:31873489-31873508 | MS.gene81124:intron | 10.0% |
| !! | ATATTTAAATATAAATGCAA+GGG | + | chr2.4:31873490-31873509 | MS.gene81124:intron | 10.0% |
| !!! | GCAACAAATTTTTTTTTTTT+TGG | - | chr2.4:31873872-31873891 | None:intergenic | 15.0% |
| !!! | TTTGTTTTTCTGAATATTAA+TGG | + | chr2.4:31874298-31874317 | MS.gene81124:intron | 15.0% |
| !! | CAATCTATGATATAATAACA+TGG | - | chr2.4:31874385-31874404 | None:intergenic | 20.0% |
| !! | CATGTTATTATATCATAGAT+TGG | + | chr2.4:31874383-31874402 | MS.gene81124:intron | 20.0% |
| !!! | AAAGCATTTATCATATTATG+AGG | - | chr2.4:31874036-31874055 | None:intergenic | 20.0% |
| !!! | TTTTTTTTTTTGGTAGAACA+GGG | - | chr2.4:31873862-31873881 | None:intergenic | 20.0% |
| !!! | TTTTTTTTTTTTGGTAGAAC+AGG | - | chr2.4:31873863-31873882 | None:intergenic | 20.0% |
| ! | AAAAAAATTGTATGAAGCTC+TGG | + | chr2.4:31873820-31873839 | MS.gene81124:intron | 25.0% |
| ! | ATTCAGAAAAACAAATGCAA+GGG | - | chr2.4:31874293-31874312 | None:intergenic | 25.0% |
| ! | CTTATGATCTAGCTAATTAA+AGG | - | chr2.4:31873915-31873934 | None:intergenic | 25.0% |
| ! | CTTTAATTAGCTAGATCATA+AGG | + | chr2.4:31873913-31873932 | MS.gene81124:intron | 25.0% |
| ! | GTAAAGCTTCAATATACTTT+CGG | - | chr2.4:31874537-31874556 | None:intergenic | 25.0% |
| ! | TATTCAGAAAAACAAATGCA+AGG | - | chr2.4:31874294-31874313 | None:intergenic | 25.0% |
| !! | TCTGGTATTTAATAGAGTTT+TGG | + | chr2.4:31873668-31873687 | MS.gene81124:intron | 25.0% |
| !! | TTGAATTTTGAACATCCAAA+TGG | - | chr2.4:31873528-31873547 | None:intergenic | 25.0% |
| !!! | CTTCTTTTGGTTATTCTATT+TGG | + | chr2.4:31873998-31874017 | MS.gene81124:intron | 25.0% |
| !!! | GATTTTAAGTTCTCATTGAT+TGG | + | chr2.4:31873610-31873629 | MS.gene81124:intron | 25.0% |
| GTAGCATTCAAGTAAAGTTA+CGG | - | chr2.4:31873722-31873741 | None:intergenic | 30.0% | |
| TAGATTGGAATGTCTTTCTT+TGG | + | chr2.4:31874398-31874417 | MS.gene81124:intron | 30.0% | |
| TCTTCTTCAACGTTTAGAAA+GGG | + | chr2.4:31874190-31874209 | MS.gene81124:CDS | 30.0% | |
| ! | CTTCAACACTTTTCTTCTTT+TGG | + | chr2.4:31873985-31874004 | MS.gene81124:intron | 30.0% |
| ! | TTGAGTAAAGTACCAACAAA+AGG | + | chr2.4:31874341-31874360 | MS.gene81124:intron | 30.0% |
| ! | TTTGTTCTTTCTCCTTTTGT+TGG | - | chr2.4:31874356-31874375 | None:intergenic | 30.0% |
| !! | GGGTGAATTATTTTTCCATT+TGG | + | chr2.4:31873510-31873529 | MS.gene81124:intron | 30.0% |
| !! | GTATTTAATAGAGTTTTGGC+TGG | + | chr2.4:31873672-31873691 | MS.gene81124:intron | 30.0% |
| !!! | TAGTCTATGAGCTTTTTCAA+AGG | - | chr2.4:31874126-31874145 | None:intergenic | 30.0% |
| CTCTTCTTCAACGTTTAGAA+AGG | + | chr2.4:31874189-31874208 | MS.gene81124:CDS | 35.0% | |
| GTATTGAAAATGACTGTGTC+TGG | + | chr2.4:31873650-31873669 | MS.gene81124:intron | 35.0% | |
| TTTACAGAATGCTGCTGATA+AGG | + | chr2.4:31874552-31874571 | MS.gene81124:CDS | 35.0% | |
| ! | AGTATTTTCCCATTCTTTGC+AGG | + | chr2.4:31874438-31874457 | MS.gene81124:intron | 35.0% |
| ! | TCTTTAAGGAGCTTCAAAGT+TGG | + | chr2.4:31873943-31873962 | MS.gene81124:intron | 35.0% |
| !!! | AGTTTTGGCTGGAAATTTCT+TGG | + | chr2.4:31873683-31873702 | MS.gene81124:intron | 35.0% |
| CATAAGGTGACAGCTCTTTA+AGG | + | chr2.4:31873929-31873948 | MS.gene81124:intron | 40.0% | |
| CTTCGACTTCATTAGCAACA+CGG | - | chr2.4:31873416-31873435 | None:intergenic | 40.0% | |
| GTCATTCTCCTGCAAAGAAT+GGG | - | chr2.4:31874449-31874468 | None:intergenic | 40.0% | |
| TCATTAGCAACACGGAGAAT+AGG | - | chr2.4:31873408-31873427 | None:intergenic | 40.0% | |
| TGTCATTCTCCTGCAAAGAA+TGG | - | chr2.4:31874450-31874469 | None:intergenic | 40.0% | |
| ! | CACTCGTCTGGTTTGTAATT+AGG | + | chr2.4:31874089-31874108 | MS.gene81124:intron | 40.0% |
| ! | CGTCTGGTTTGTAATTAGGT+CGG | + | chr2.4:31874093-31874112 | MS.gene81124:intron | 40.0% |
| ACGACTTCACTGTCCAGAAT+CGG | - | chr2.4:31873360-31873379 | None:intergenic | 45.0% | |
| AGACTAGATCCCACATCTAG+TGG | + | chr2.4:31874140-31874159 | MS.gene81124:CDS | 45.0% | |
| GACCCCCTTCTATAAGACAT+CGG | - | chr2.4:31873276-31873295 | None:intergenic | 45.0% | |
| GACTAGATCCCACATCTAGT+GGG | + | chr2.4:31874141-31874160 | MS.gene81124:CDS | 45.0% | |
| GAGAATGACACCACTTTGGA+GGG | + | chr2.4:31874460-31874479 | MS.gene81124:CDS | 45.0% | |
| GCGATCTCAACCAATGAAGA+TGG | - | chr2.4:31873384-31873403 | None:intergenic | 45.0% | |
| TGAAGTCGTGCCATCTTCAT+TGG | + | chr2.4:31873371-31873390 | MS.gene81124:CDS | 45.0% | |
| !! | TTATATTTAAATATTTAAAG+AGG | - | chr2.4:31873484-31873503 | None:intergenic | 5.0% |
| ACTCAGACTGCCGGGAATTT+AGG | + | chr2.4:31873327-31873346 | MS.gene81124:CDS | 50.0% | |
| CGACTTCACTGTCCAGAATC+GGG | - | chr2.4:31873359-31873378 | None:intergenic | 50.0% | |
| CTCTGTCTAATGCACTCGTC+TGG | + | chr2.4:31874077-31874096 | MS.gene81124:intron | 50.0% | |
| CTTTGCTCTTCCCTCCAAAG+TGG | - | chr2.4:31874473-31874492 | None:intergenic | 50.0% | |
| GCAGGAGAATGACACCACTT+TGG | + | chr2.4:31874456-31874475 | MS.gene81124:intron | 50.0% | |
| GGAGAATGACACCACTTTGG+AGG | + | chr2.4:31874459-31874478 | MS.gene81124:intron | 50.0% | |
| TTTAGGAGCAGACCCGATTC+TGG | + | chr2.4:31873344-31873363 | MS.gene81124:CDS | 50.0% | |
| AACACCACGCCCACTAGATG+TGG | - | chr2.4:31874153-31874172 | None:intergenic | 55.0% | |
| ACACCACGCCCACTAGATGT+GGG | - | chr2.4:31874152-31874171 | None:intergenic | 55.0% | |
| ATACTGCGGACTCAGACTGC+CGG | + | chr2.4:31873318-31873337 | MS.gene81124:CDS | 55.0% | |
| CGGGTCTGCTCCTAAATTCC+CGG | - | chr2.4:31873340-31873359 | None:intergenic | 55.0% | |
| TACTGCGGACTCAGACTGCC+GGG | + | chr2.4:31873319-31873338 | MS.gene81124:CDS | 60.0% | |
| ! | GATCCCACATCTAGTGGGCG+TGG | + | chr2.4:31874146-31874165 | MS.gene81124:CDS | 60.0% |
| AACCGCAGCGGCGGATACTG+CGG | + | chr2.4:31873304-31873323 | MS.gene81124:CDS | 65.0% | |
| GGTCTGAGCAGCAACCGCAG+CGG | + | chr2.4:31873292-31873311 | MS.gene81124:CDS | 65.0% | |
| CTGAGCAGCAACCGCAGCGG+CGG | + | chr2.4:31873295-31873314 | MS.gene81124:CDS | 70.0% | |
| GTCCGCAGTATCCGCCGCTG+CGG | - | chr2.4:31873309-31873328 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 31873276 | 31874578 | 31873276 | ID=MS.gene81124 |
| chr2.4 | mRNA | 31873276 | 31874578 | 31873276 | ID=MS.gene81124.t1;Parent=MS.gene81124 |
| chr2.4 | exon | 31873276 | 31873462 | 31873276 | ID=MS.gene81124.t1.exon1;Parent=MS.gene81124.t1 |
| chr2.4 | CDS | 31873276 | 31873462 | 31873276 | ID=cds.MS.gene81124.t1;Parent=MS.gene81124.t1 |
| chr2.4 | exon | 31874111 | 31874211 | 31874111 | ID=MS.gene81124.t1.exon2;Parent=MS.gene81124.t1 |
| chr2.4 | CDS | 31874111 | 31874211 | 31874111 | ID=cds.MS.gene81124.t1;Parent=MS.gene81124.t1 |
| chr2.4 | exon | 31874460 | 31874578 | 31874460 | ID=MS.gene81124.t1.exon3;Parent=MS.gene81124.t1 |
| chr2.4 | CDS | 31874460 | 31874578 | 31874460 | ID=cds.MS.gene81124.t1;Parent=MS.gene81124.t1 |
| Gene Sequence |
| Protein sequence |
