AlfalfaGEDB | MS.gene81546

M. sativa cultivar XinJiangDaYe / MS.gene81546

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81546.t1	XP_013464057.1	95	141	7	0	1	141	1	141	5.40E-73	283.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81546.t1	A0A072V8C8	95.0	141	7	0	1	141	1	141	3.9e-73	283.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050170	MS.gene81546	-0.821324	4.29E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81546.t1	MTR_2g461190	95.035	141	7	0	1	141	1	141	7.49e-98	277
MS.gene81546.t1	MTR_4g116530	58.667	150	50	5	1	140	1	148	5.25e-49	154
MS.gene81546.t1	MTR_6g007727	46.154	78	33	3	59	130	63	137	4.93e-14	65.5
MS.gene81546.t1	MTR_3g078240	46.667	75	34	3	66	134	51	125	1.20e-13	63.9
MS.gene81546.t1	MTR_4g106650	46.835	79	35	4	57	130	55	131	2.71e-13	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81546.t1	AT5G25240	39.669	121	58	5	21	131	11	126	3.43e-15	67.8
MS.gene81546.t1	AT3G01430	30.061	163	73	6	5	131	4	161	5.02e-15	68.6
MS.gene81546.t1	AT5G14895	40.449	89	40	3	56	131	60	148	1.32e-13	64.7

Find 49 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCGTGTTTGTGGGTTGAAA+TGG	0.310334	2.2:+33474665	MS.gene81546:CDS
CTTTCCCTCTGGACGTCGCC+AGG	0.321304	2.2:+33474957	MS.gene81546:CDS
CATAGCTGTGTTCATCATAT+TGG	0.336315	2.2:-33474858	None:intergenic
TCGTGTTTGTGGGTTGAAAT+GGG	0.336472	2.2:+33474666	MS.gene81546:CDS
GAGGCTAGTGGATGTGGGTA+TGG	0.396753	2.2:+33474637	MS.gene81546:CDS
CACCAAGAGGCTAGTGGATG+TGG	0.398723	2.2:+33474631	MS.gene81546:CDS
TGGAGCAAAGGGAAGATTCT+TGG	0.402551	2.2:+33474719	MS.gene81546:CDS
ATTAGAAAGATTAGCATGTA+TGG	0.403666	2.2:+33474811	MS.gene81546:CDS
TATGGGTGCTTTCGTGTTTG+TGG	0.415294	2.2:+33474655	MS.gene81546:CDS
ATGGGTGCTTTCGTGTTTGT+GGG	0.418529	2.2:+33474656	MS.gene81546:CDS
AAGCCCATTGGTTGAGGCGG+TGG	0.425680	2.2:+33474582	MS.gene81546:CDS
TTTAGTGCTCCCTTTCCCTC+TGG	0.450569	2.2:+33474946	MS.gene81546:CDS
TGTTCCACCGCCTCAACCAA+TGG	0.453542	2.2:-33474586	None:intergenic
GAGGTTTCAGAAGTGATTGC+AGG	0.459250	2.2:+33474769	MS.gene81546:CDS
CCTTTACCTTCATCACTGCT+TGG	0.460194	2.2:-33474691	None:intergenic
CTAATGAATGTCTTCCACTT+AGG	0.465024	2.2:-33474793	None:intergenic
CTGGCGACGTCCAGAGGGAA+AGG	0.473742	2.2:-33474956	None:intergenic
TGAGGGAAGCCCATTGGTTG+AGG	0.474777	2.2:+33474576	MS.gene81546:CDS
TGATGAAGGTAAAGGTCTGC+TGG	0.481616	2.2:+33474699	MS.gene81546:CDS
CGTGTTTGTGGGTTGAAATG+GGG	0.493007	2.2:+33474667	MS.gene81546:CDS
TTGCAAGTTGAGGAAGATGA+AGG	0.493022	2.2:+33474747	MS.gene81546:CDS
ACCCACATCCACTAGCCTCT+TGG	0.506045	2.2:-33474633	None:intergenic
GTTCCACCGCCTCAACCAAT+GGG	0.506673	2.2:-33474585	None:intergenic
TTTCCCTCTGGACGTCGCCA+GGG	0.510048	2.2:+33474958	MS.gene81546:CDS
ATGCTCTCAACTTCAGTAGT+GGG	0.511718	2.2:+33474878	MS.gene81546:CDS
TAAAGGTCTGCTGGAGCAAA+GGG	0.516320	2.2:+33474708	MS.gene81546:CDS
CTTGGTGGGTTTGCAAGTTG+AGG	0.524199	2.2:+33474737	MS.gene81546:CDS
CAAGTTGAGGAAGATGAAGG+AGG	0.525977	2.2:+33474750	MS.gene81546:CDS
GTGTTTGTGGGTTGAAATGG+GGG	0.532525	2.2:+33474668	MS.gene81546:CDS
TTCACCCTGGCGACGTCCAG+AGG	0.534141	2.2:-33474962	None:intergenic
AGGCTAGTGGATGTGGGTAT+GGG	0.540821	2.2:+33474638	MS.gene81546:CDS
GTAAAGGTCTGCTGGAGCAA+AGG	0.550373	2.2:+33474707	MS.gene81546:CDS
TGGCGACGTCCAGAGGGAAA+GGG	0.567294	2.2:-33474955	None:intergenic
AAGTGATTGCAGGTCCTAAG+TGG	0.573683	2.2:+33474779	MS.gene81546:CDS
TATGCTCTCAACTTCAGTAG+TGG	0.578055	2.2:+33474877	MS.gene81546:CDS
GCAAAGGGAAGATTCTTGGT+GGG	0.584946	2.2:+33474723	MS.gene81546:CDS
TGCTCTCAACTTCAGTAGTG+GGG	0.585014	2.2:+33474879	MS.gene81546:CDS
CCAAGCAGTGATGAAGGTAA+AGG	0.588857	2.2:+33474691	MS.gene81546:CDS
GCGGTGGAACACGGTAGAGA+AGG	0.590440	2.2:+33474598	MS.gene81546:CDS
AATGATCACCAAGAGGCTAG+TGG	0.598393	2.2:+33474625	MS.gene81546:CDS
GGGAAGCCCATTGGTTGAGG+CGG	0.598814	2.2:+33474579	MS.gene81546:CDS
TTGGTTGAGGCGGTGGAACA+CGG	0.607703	2.2:+33474589	MS.gene81546:CDS
AGCAAAGGGAAGATTCTTGG+TGG	0.616895	2.2:+33474722	MS.gene81546:CDS
GTATGGTAAGAAGCAACAGA+AGG	0.619307	2.2:+33474828	MS.gene81546:CDS
TCACCCTGGCGACGTCCAGA+GGG	0.620675	2.2:-33474961	None:intergenic
ACCAAGAGGCTAGTGGATGT+GGG	0.624825	2.2:+33474632	MS.gene81546:CDS
TATGGTAAGAAGCAACAGAA+GGG	0.629658	2.2:+33474829	MS.gene81546:CDS
GCATGAATCACAGTTCACCC+TGG	0.660347	2.2:-33474975	None:intergenic
AGGTTTCAATGATCACCAAG+AGG	0.706746	2.2:+33474618	MS.gene81546:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTAGAAAGATTAGCATGTA+TGG	+	chr2.2:33474811-33474830	MS.gene81546:CDS	25.0%
	CATAGCTGTGTTCATCATAT+TGG	-	chr2.2:33474861-33474880	None:intergenic	35.0%
	CTAATGAATGTCTTCCACTT+AGG	-	chr2.2:33474796-33474815	None:intergenic	35.0%
	TATGGTAAGAAGCAACAGAA+GGG	+	chr2.2:33474829-33474848	MS.gene81546:CDS	35.0%
	AGGTTTCAATGATCACCAAG+AGG	+	chr2.2:33474618-33474637	MS.gene81546:CDS	40.0%
	ATGCTCTCAACTTCAGTAGT+GGG	+	chr2.2:33474878-33474897	MS.gene81546:CDS	40.0%
	GTATGGTAAGAAGCAACAGA+AGG	+	chr2.2:33474828-33474847	MS.gene81546:CDS	40.0%
	TATGCTCTCAACTTCAGTAG+TGG	+	chr2.2:33474877-33474896	MS.gene81546:CDS	40.0%
!	TCGTGTTTGTGGGTTGAAAT+GGG	+	chr2.2:33474666-33474685	MS.gene81546:CDS	40.0%
!	TTCGTGTTTGTGGGTTGAAA+TGG	+	chr2.2:33474665-33474684	MS.gene81546:CDS	40.0%
!	TTGCAAGTTGAGGAAGATGA+AGG	+	chr2.2:33474747-33474766	MS.gene81546:CDS	40.0%
	AACCTAGCCGAAAAACTGTG+CGG	-	chr2.2:33474928-33474947	None:intergenic	45.0%
	AAGTGATTGCAGGTCCTAAG+TGG	+	chr2.2:33474779-33474798	MS.gene81546:CDS	45.0%
	AATGATCACCAAGAGGCTAG+TGG	+	chr2.2:33474625-33474644	MS.gene81546:CDS	45.0%
	AGCAAAGGGAAGATTCTTGG+TGG	+	chr2.2:33474722-33474741	MS.gene81546:CDS	45.0%
	ATGGGTGCTTTCGTGTTTGT+GGG	+	chr2.2:33474656-33474675	MS.gene81546:CDS	45.0%
	CCAAGCAGTGATGAAGGTAA+AGG	+	chr2.2:33474691-33474710	MS.gene81546:CDS	45.0%
	CCTTTACCTTCATCACTGCT+TGG	-	chr2.2:33474694-33474713	None:intergenic	45.0%
	GAGGTTTCAGAAGTGATTGC+AGG	+	chr2.2:33474769-33474788	MS.gene81546:CDS	45.0%
	GCAAAGGGAAGATTCTTGGT+GGG	+	chr2.2:33474723-33474742	MS.gene81546:CDS	45.0%
	TAAAGGTCTGCTGGAGCAAA+GGG	+	chr2.2:33474708-33474727	MS.gene81546:CDS	45.0%
	TATGGGTGCTTTCGTGTTTG+TGG	+	chr2.2:33474655-33474674	MS.gene81546:CDS	45.0%
	TGATGAAGGTAAAGGTCTGC+TGG	+	chr2.2:33474699-33474718	MS.gene81546:CDS	45.0%
	TGCTCTCAACTTCAGTAGTG+GGG	+	chr2.2:33474879-33474898	MS.gene81546:CDS	45.0%
	TGGAGCAAAGGGAAGATTCT+TGG	+	chr2.2:33474719-33474738	MS.gene81546:CDS	45.0%
!	CAAGTTGAGGAAGATGAAGG+AGG	+	chr2.2:33474750-33474769	MS.gene81546:CDS	45.0%
!	CGTGTTTGTGGGTTGAAATG+GGG	+	chr2.2:33474667-33474686	MS.gene81546:CDS	45.0%
!	GTGTTTGTGGGTTGAAATGG+GGG	+	chr2.2:33474668-33474687	MS.gene81546:CDS	45.0%
	GTAAAGGTCTGCTGGAGCAA+AGG	+	chr2.2:33474707-33474726	MS.gene81546:CDS	50.0%
	TTTAGTGCTCCCTTTCCCTC+TGG	+	chr2.2:33474946-33474965	MS.gene81546:CDS	50.0%
!	ACCAAGAGGCTAGTGGATGT+GGG	+	chr2.2:33474632-33474651	MS.gene81546:CDS	50.0%
!	AGGCTAGTGGATGTGGGTAT+GGG	+	chr2.2:33474638-33474657	MS.gene81546:CDS	50.0%
!	CGACTTGCCGCACAGTTTTT+CGG	+	chr2.2:33474918-33474937	MS.gene81546:CDS	50.0%
!!	CTTGGTGGGTTTGCAAGTTG+AGG	+	chr2.2:33474737-33474756	MS.gene81546:CDS	50.0%
!!	TGCTTTTGAGGGAAGCCCAT+TGG	+	chr2.2:33474570-33474589	MS.gene81546:CDS	50.0%
	GTTCCACCGCCTCAACCAAT+GGG	-	chr2.2:33474588-33474607	None:intergenic	55.0%
	TGTTCCACCGCCTCAACCAA+TGG	-	chr2.2:33474589-33474608	None:intergenic	55.0%
	TTGGTTGAGGCGGTGGAACA+CGG	+	chr2.2:33474589-33474608	MS.gene81546:CDS	55.0%
!	ACCCACATCCACTAGCCTCT+TGG	-	chr2.2:33474636-33474655	None:intergenic	55.0%
!	CACCAAGAGGCTAGTGGATG+TGG	+	chr2.2:33474631-33474650	MS.gene81546:CDS	55.0%
!	GAGGCTAGTGGATGTGGGTA+TGG	+	chr2.2:33474637-33474656	MS.gene81546:CDS	55.0%
!	TGAGGGAAGCCCATTGGTTG+AGG	+	chr2.2:33474576-33474595	MS.gene81546:CDS	55.0%
!!	TGCCGCACAGTTTTTCGGCT+AGG	+	chr2.2:33474923-33474942	MS.gene81546:CDS	55.0%
	GCGGTGGAACACGGTAGAGA+AGG	+	chr2.2:33474598-33474617	MS.gene81546:CDS	60.0%
	TGGCGACGTCCAGAGGGAAA+GGG	-	chr2.2:33474958-33474977	None:intergenic	60.0%
	TGGGGGCCAAGCAGTGATGA+AGG	+	chr2.2:33474685-33474704	MS.gene81546:CDS	60.0%
	TTTCCCTCTGGACGTCGCCA+GGG	+	chr2.2:33474958-33474977	MS.gene81546:CDS	60.0%
!	AAGCCCATTGGTTGAGGCGG+TGG	+	chr2.2:33474582-33474601	MS.gene81546:CDS	60.0%
!	GGGAAGCCCATTGGTTGAGG+CGG	+	chr2.2:33474579-33474598	MS.gene81546:CDS	60.0%
	CTGGCGACGTCCAGAGGGAA+AGG	-	chr2.2:33474959-33474978	None:intergenic	65.0%
	CTTTCCCTCTGGACGTCGCC+AGG	+	chr2.2:33474957-33474976	MS.gene81546:CDS	65.0%
	TCACCCTGGCGACGTCCAGA+GGG	-	chr2.2:33474964-33474983	None:intergenic	65.0%
	TTCACCCTGGCGACGTCCAG+AGG	-	chr2.2:33474965-33474984	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	33474565	33474990	33474565	ID=MS.gene81546
chr2.2	mRNA	33474565	33474990	33474565	ID=MS.gene81546.t1;Parent=MS.gene81546
chr2.2	exon	33474565	33474990	33474565	ID=MS.gene81546.t1.exon1;Parent=MS.gene81546.t1
chr2.2	CDS	33474565	33474990	33474565	ID=cds.MS.gene81546.t1;Parent=MS.gene81546.t1

Gene Sequence

>MS.gene81546

ATGGCTGCTTTTGAGGGAAGCCCATTGGTTGAGGCGGTGGAACACGGTAGAGAAGGTTTCAATGATCACCAAGAGGCTAGTGGATGTGGGTATGGGTGCTTTCGTGTTTGTGGGTTGAAATGGGGGCCAAGCAGTGATGAAGGTAAAGGTCTGCTGGAGCAAAGGGAAGATTCTTGGTGGGTTTGCAAGTTGAGGAAGATGAAGGAGGTTTCAGAAGTGATTGCAGGTCCTAAGTGGAAGACATTCATTAGAAAGATTAGCATGTATGGTAAGAAGCAACAGAAGGGTAAGTTCCAATATGATGAACACAGCTATGCTCTCAACTTCAGTAGTGGGGCTCAGAGTGAAGATGACGACTTGCCGCACAGTTTTTCGGCTAGGTTTAGTGCTCCCTTTCCCTCTGGACGTCGCCAGGGTGAACTGTGA

Protein sequence

>MS.gene81546.t1

MAAFEGSPLVEAVEHGREGFNDHQEASGCGYGCFRVCGLKWGPSSDEGKGLLEQREDSWWVCKLRKMKEVSEVIAGPKWKTFIRKISMYGKKQQKGKFQYDEHSYALNFSSGAQSEDDDLPHSFSARFSAPFPSGRRQGEL