AlfalfaGEDB | MS.gene81716

M. sativa cultivar XinJiangDaYe / MS.gene81716

KEGG: K02867
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00298.22 PF03946.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81716.t1	XP_013468029.1	100	149	0	0	1	149	1	149	6.50E-77	296.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81716.t1	Q9UTK2	51.9	135	65	0	11	145	9	143	5.8e-34	145.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81716.t1	A0A072VKA9	100.0	149	0	0	1	149	1	149	4.7e-77	296.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene056973	MS.gene81716	-0.817403	3.36E-52	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene045480	MS.gene81716	PPI
MS.gene006700	MS.gene81716	PPI
MS.gene73862	MS.gene81716	PPI
MS.gene031594	MS.gene81716	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81716.t1	MTR_1g060610	100.000	149	0	0	1	149	1	149	4.81e-109	306
MS.gene81716.t1	MTR_3g080800	44.286	140	75	2	5	144	66	202	1.26e-28	105

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene81716.t1	AT4G35490	85.315	143	21	0	7	149	13	155	1.15e-91	263
MS.gene81716.t1	AT4G35490	85.315	143	21	0	7	149	13	155	1.15e-91	263
MS.gene81716.t1	AT1G32990	44.286	140	75	2	5	144	72	208	7.44e-28	103

Find 54 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCCATTTCTAAATCCATTAT+TGG	0.175466	1.3:+43992549	MS.gene81716:CDS
GTAACAGCCATTGGTGTATC+AGG	0.275383	1.3:-43992324	None:intergenic
CGATACCCGCAGCTTTCTTC+AGG	0.313010	1.3:-43992410	None:intergenic
GCTCCTAGCTGTACCAATAA+TGG	0.322397	1.3:-43992562	None:intergenic
ACAGTCTGATCCTTACTTGC+AGG	0.357839	1.3:+43992512	MS.gene81716:CDS
ACCAATAATGGATTTAGAAA+TGG	0.398795	1.3:-43992550	None:intergenic
GAAAGCTGCGGGTATCGAAT+TGG	0.398936	1.3:+43992416	MS.gene81716:CDS
GGAGGTGGCGGTCACATGAC+CGG	0.412632	1.3:-43992451	None:intergenic
ATTGTCGCTGCTACCACTTT+CGG	0.429301	1.3:-43992177	None:intergenic
AGTGGCATACCCTGCAAGTA+AGG	0.435747	1.3:-43992522	None:intergenic
AACGATGAGCGTTCCGAAAG+TGG	0.442624	1.3:+43992164	None:intergenic
CCTCCTGTGGGACCTGCGCT+AGG	0.444053	1.3:+43992237	MS.gene81716:CDS
CTCCAGCTGGAACTCTCAAC+CGG	0.454096	1.3:-43992200	None:intergenic
TCCAGCTGGAGGAGCACGTC+CGG	0.454983	1.3:+43992212	MS.gene81716:CDS
AGCACGTCCGGCGCCTCCTG+TGG	0.466575	1.3:+43992224	MS.gene81716:CDS
AAAGCTGCGGGTATCGAATT+GGG	0.473529	1.3:+43992417	MS.gene81716:CDS
AGTCTTTGCAGAAAGCCATA+AGG	0.492679	1.3:-43992275	None:intergenic
GCTGTTATTGTAACAGCCAT+TGG	0.492932	1.3:-43992333	None:intergenic
GGCGGTCACATGACCGGGAC+GGG	0.494244	1.3:-43992445	None:intergenic
TCAGTACCGACTGAACCTTA+TGG	0.497875	1.3:+43992260	MS.gene81716:CDS
AAGTGGTAGCAGCGACAATC+CGG	0.509428	1.3:+43992181	MS.gene81716:CDS
GCCGGACGTGCTCCTCCAGC+TGG	0.512277	1.3:-43992213	None:intergenic
GCACGTCCGGCGCCTCCTGT+GGG	0.517535	1.3:+43992225	MS.gene81716:CDS
CATACACGTGCCGAAGTGAC+AGG	0.517596	1.3:-43992473	None:intergenic
GAGGTGGCGGTCACATGACC+GGG	0.519759	1.3:-43992450	None:intergenic
CCGAAGTGACAGGGAGGTGG+CGG	0.522973	1.3:-43992463	None:intergenic
TGTTACAATAACAGCTTACA+AGG	0.549953	1.3:+43992341	MS.gene81716:CDS
TGACCTAGCGCAGGTCCCAC+AGG	0.552959	1.3:-43992240	None:intergenic
GCAGGTCCCACAGGAGGCGC+CGG	0.567732	1.3:-43992231	None:intergenic
ATTGGTACAGCTAGGAGCAT+GGG	0.571876	1.3:+43992567	MS.gene81716:CDS
CCGCCACCTCCCTGTCACTT+CGG	0.584871	1.3:+43992463	MS.gene81716:CDS
TGGTACCTGAAGAAAGCTGC+GGG	0.584881	1.3:+43992405	MS.gene81716:CDS
ATCCGGTTGAGAGTTCCAGC+TGG	0.592293	1.3:+43992198	MS.gene81716:CDS
TATTGGTACAGCTAGGAGCA+TGG	0.593721	1.3:+43992566	MS.gene81716:CDS
CAGTCTGATCCTTACTTGCA+GGG	0.597928	1.3:+43992513	MS.gene81716:CDS
GTGCCGAAGTGACAGGGAGG+TGG	0.602083	1.3:-43992466	None:intergenic
TTAAATCTCCTTCCGTCTCG+TGG	0.602388	1.3:+43992385	MS.gene81716:CDS
TTCAGGTACCACGAGACGGA+AGG	0.605506	1.3:-43992393	None:intergenic
TGGCGGTCACATGACCGGGA+CGG	0.607191	1.3:-43992446	None:intergenic
TTTCTTCAGGTACCACGAGA+CGG	0.610355	1.3:-43992397	None:intergenic
AGATTTAACAGTGAATTCAA+AGG	0.616903	1.3:-43992370	None:intergenic
AGTCGGTACTGACCTAGCGC+AGG	0.626095	1.3:-43992249	None:intergenic
GAATTGGGAAGTACCCGTCC+CGG	0.626450	1.3:+43992432	MS.gene81716:CDS
AATCCATTATTGGTACAGCT+AGG	0.638443	1.3:+43992559	MS.gene81716:CDS
CCTAGCGCAGGTCCCACAGG+AGG	0.638505	1.3:-43992237	None:intergenic
ATACACGTGCCGAAGTGACA+GGG	0.645886	1.3:-43992472	None:intergenic
AGAAAGCCATAAGGTTCAGT+CGG	0.648161	1.3:-43992266	None:intergenic
TCTGCAAAGACTTCAACGCA+CGG	0.650245	1.3:+43992286	MS.gene81716:CDS
GATTTAGAAATGGACTCAAG+TGG	0.655714	1.3:-43992540	None:intergenic
ATTCAAGCCTGATACACCAA+TGG	0.658568	1.3:+43992317	MS.gene81716:CDS
GTGGTACCTGAAGAAAGCTG+CGG	0.662466	1.3:+43992404	MS.gene81716:CDS
CGGTTGAGAGTTCCAGCTGG+AGG	0.663118	1.3:+43992201	MS.gene81716:CDS
GCACGTGTATGAGATCGCTA+AGG	0.664058	1.3:+43992485	MS.gene81716:CDS
CACGTGCCGAAGTGACAGGG+AGG	0.790794	1.3:-43992469	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGATTTAACAGTGAATTCAA+AGG	-	chr1.3:43992373-43992392	None:intergenic	25.0%
!	TCCATTTCTAAATCCATTAT+TGG	+	chr1.3:43992549-43992568	MS.gene81716:CDS	25.0%
!!!	ACCAATAATGGATTTAGAAA+TGG	-	chr1.3:43992553-43992572	None:intergenic	25.0%
	TGTTACAATAACAGCTTACA+AGG	+	chr1.3:43992341-43992360	MS.gene81716:CDS	30.0%
	AATCCATTATTGGTACAGCT+AGG	+	chr1.3:43992559-43992578	MS.gene81716:CDS	35.0%
!	GATTTAGAAATGGACTCAAG+TGG	-	chr1.3:43992543-43992562	None:intergenic	35.0%
	AGAAAGCCATAAGGTTCAGT+CGG	-	chr1.3:43992269-43992288	None:intergenic	40.0%
	AGTCTTTGCAGAAAGCCATA+AGG	-	chr1.3:43992278-43992297	None:intergenic	40.0%
	ATTCAAGCCTGATACACCAA+TGG	+	chr1.3:43992317-43992336	MS.gene81716:CDS	40.0%
	GCTGTTATTGTAACAGCCAT+TGG	-	chr1.3:43992336-43992355	None:intergenic	40.0%
	ACAGTCTGATCCTTACTTGC+AGG	+	chr1.3:43992512-43992531	MS.gene81716:CDS	45.0%
	ATTGTCGCTGCTACCACTTT+CGG	-	chr1.3:43992180-43992199	None:intergenic	45.0%
	CAGTCTGATCCTTACTTGCA+GGG	+	chr1.3:43992513-43992532	MS.gene81716:CDS	45.0%
	GCTCCTAGCTGTACCAATAA+TGG	-	chr1.3:43992565-43992584	None:intergenic	45.0%
	GTAACAGCCATTGGTGTATC+AGG	-	chr1.3:43992327-43992346	None:intergenic	45.0%
	TATTGGTACAGCTAGGAGCA+TGG	+	chr1.3:43992566-43992585	MS.gene81716:CDS	45.0%
	TCTGCAAAGACTTCAACGCA+CGG	+	chr1.3:43992286-43992305	MS.gene81716:CDS	45.0%
	TTAAATCTCCTTCCGTCTCG+TGG	+	chr1.3:43992385-43992404	MS.gene81716:CDS	45.0%
	TTTCTTCAGGTACCACGAGA+CGG	-	chr1.3:43992400-43992419	None:intergenic	45.0%
!	ATTGGTACAGCTAGGAGCAT+GGG	+	chr1.3:43992567-43992586	MS.gene81716:CDS	45.0%
!!	AAAGCTGCGGGTATCGAATT+GGG	+	chr1.3:43992417-43992436	MS.gene81716:CDS	45.0%
!!	TCAGTACCGACTGAACCTTA+TGG	+	chr1.3:43992260-43992279	MS.gene81716:CDS	45.0%
	AAGTGGTAGCAGCGACAATC+CGG	+	chr1.3:43992181-43992200	MS.gene81716:CDS	50.0%
	AGTGGCATACCCTGCAAGTA+AGG	-	chr1.3:43992525-43992544	None:intergenic	50.0%
	ATACACGTGCCGAAGTGACA+GGG	-	chr1.3:43992475-43992494	None:intergenic	50.0%
	GCACGTGTATGAGATCGCTA+AGG	+	chr1.3:43992485-43992504	MS.gene81716:CDS	50.0%
	GTGGTACCTGAAGAAAGCTG+CGG	+	chr1.3:43992404-43992423	MS.gene81716:CDS	50.0%
	TGGTACCTGAAGAAAGCTGC+GGG	+	chr1.3:43992405-43992424	MS.gene81716:CDS	50.0%
!!	GAAAGCTGCGGGTATCGAAT+TGG	+	chr1.3:43992416-43992435	MS.gene81716:CDS	50.0%
	ATCCGGTTGAGAGTTCCAGC+TGG	+	chr1.3:43992198-43992217	MS.gene81716:CDS	55.0%
	CATACACGTGCCGAAGTGAC+AGG	-	chr1.3:43992476-43992495	None:intergenic	55.0%
	CGATACCCGCAGCTTTCTTC+AGG	-	chr1.3:43992413-43992432	None:intergenic	55.0%
	CTCCAGCTGGAACTCTCAAC+CGG	-	chr1.3:43992203-43992222	None:intergenic	55.0%
	TTCAGGTACCACGAGACGGA+AGG	-	chr1.3:43992396-43992415	None:intergenic	55.0%
!!	GAATTGGGAAGTACCCGTCC+CGG	+	chr1.3:43992432-43992451	MS.gene81716:CDS	55.0%
	CGGTTGAGAGTTCCAGCTGG+AGG	+	chr1.3:43992201-43992220	MS.gene81716:CDS	60.0%
!!	AGTCGGTACTGACCTAGCGC+AGG	-	chr1.3:43992252-43992271	None:intergenic	60.0%
	CACGTGCCGAAGTGACAGGG+AGG	-	chr1.3:43992472-43992491	None:intergenic	65.0%
	CCGAAGTGACAGGGAGGTGG+CGG	-	chr1.3:43992466-43992485	None:intergenic	65.0%
	GTGCCGAAGTGACAGGGAGG+TGG	-	chr1.3:43992469-43992488	None:intergenic	65.0%
	TGACCTAGCGCAGGTCCCAC+AGG	-	chr1.3:43992243-43992262	None:intergenic	65.0%
	TGGCGGTCACATGACCGGGA+CGG	-	chr1.3:43992449-43992468	None:intergenic	65.0%
!	CCGCCACCTCCCTGTCACTT+CGG	+	chr1.3:43992463-43992482	MS.gene81716:CDS	65.0%
!	GAGGTGGCGGTCACATGACC+GGG	-	chr1.3:43992453-43992472	None:intergenic	65.0%
!	GGAGGTGGCGGTCACATGAC+CGG	-	chr1.3:43992454-43992473	None:intergenic	65.0%
!	TCCAGCTGGAGGAGCACGTC+CGG	+	chr1.3:43992212-43992231	MS.gene81716:CDS	65.0%
	CCTAGCGCAGGTCCCACAGG+AGG	-	chr1.3:43992240-43992259	None:intergenic	70.0%
	CCTCCTGTGGGACCTGCGCT+AGG	+	chr1.3:43992237-43992256	MS.gene81716:CDS	70.0%
	GGCGGTCACATGACCGGGAC+GGG	-	chr1.3:43992448-43992467	None:intergenic	70.0%
	AGCACGTCCGGCGCCTCCTG+TGG	+	chr1.3:43992224-43992243	MS.gene81716:CDS	75.0%
	GCACGTCCGGCGCCTCCTGT+GGG	+	chr1.3:43992225-43992244	MS.gene81716:CDS	75.0%
	GCCGGACGTGCTCCTCCAGC+TGG	-	chr1.3:43992216-43992235	None:intergenic	75.0%
!	GCAGGTCCCACAGGAGGCGC+CGG	-	chr1.3:43992234-43992253	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	43992168	43992617	43992168	ID=MS.gene81716
chr1.3	mRNA	43992168	43992617	43992168	ID=MS.gene81716.t1;Parent=MS.gene81716
chr1.3	exon	43992168	43992617	43992168	ID=MS.gene81716.t1.exon1;Parent=MS.gene81716.t1
chr1.3	CDS	43992168	43992617	43992168	ID=cds.MS.gene81716.t1;Parent=MS.gene81716.t1

Gene Sequence

>MS.gene81716

ATGAGCGTTCCGAAAGTGGTAGCAGCGACAATCCGGTTGAGAGTTCCAGCTGGAGGAGCACGTCCGGCGCCTCCTGTGGGACCTGCGCTAGGTCAGTACCGACTGAACCTTATGGCTTTCTGCAAAGACTTCAACGCACGGACTCAGAAATTCAAGCCTGATACACCAATGGCTGTTACAATAACAGCTTACAAGGACAACACCTTTGAATTCACTGTTAAATCTCCTTCCGTCTCGTGGTACCTGAAGAAAGCTGCGGGTATCGAATTGGGAAGTACCCGTCCCGGTCATGTGACCGCCACCTCCCTGTCACTTCGGCACGTGTATGAGATCGCTAAGGTGAAACAGTCTGATCCTTACTTGCAGGGTATGCCACTTGAGTCCATTTCTAAATCCATTATTGGTACAGCTAGGAGCATGGGAATCAAAATTGTCAAAGATCTTGAATGA

Protein sequence

>MS.gene81716.t1

MSVPKVVAATIRLRVPAGGARPAPPVGPALGQYRLNLMAFCKDFNARTQKFKPDTPMAVTITAYKDNTFEFTVKSPSVSWYLKKAAGIELGSTRPGHVTATSLSLRHVYEIAKVKQSDPYLQGMPLESISKSIIGTARSMGIKIVKDLE