Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81921.t1 | PNX89585.1 | 80.6 | 232 | 37 | 3 | 56 | 281 | 4 | 233 | 2.00E-101 | 379 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81921.t1 | F4HQ23 | 31.8 | 223 | 129 | 10 | 58 | 267 | 254 | 466 | 1.6e-14 | 81.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81921.t1 | A0A2K3MFL6 | 80.6 | 232 | 37 | 3 | 56 | 281 | 4 | 233 | 1.5e-101 | 379.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05376 | MS.gene81921 | 0.837841 | 4.10E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81921.t1 | MTR_6g082940 | 61.654 | 266 | 94 | 3 | 16 | 276 | 1 | 263 | 1.22e-108 | 320 |
| MS.gene81921.t1 | MTR_6g082920 | 61.654 | 266 | 94 | 3 | 16 | 276 | 1 | 263 | 1.22e-108 | 320 |
| MS.gene81921.t1 | MTR_6g082820 | 63.218 | 261 | 89 | 2 | 16 | 272 | 1 | 258 | 1.82e-108 | 318 |
| MS.gene81921.t1 | MTR_6g083000 | 58.621 | 261 | 102 | 2 | 16 | 272 | 1 | 259 | 3.65e-101 | 300 |
| MS.gene81921.t1 | MTR_7g082533 | 28.992 | 238 | 140 | 7 | 60 | 280 | 40 | 265 | 1.51e-26 | 106 |
| MS.gene81921.t1 | MTR_3g106280 | 39.474 | 152 | 64 | 5 | 86 | 237 | 2 | 125 | 6.21e-22 | 89.4 |
| MS.gene81921.t1 | MTR_7g082540 | 27.381 | 252 | 158 | 7 | 22 | 267 | 1 | 233 | 9.18e-20 | 87.0 |
| MS.gene81921.t1 | MTR_8g104520 | 29.167 | 264 | 156 | 13 | 25 | 275 | 5 | 250 | 2.76e-19 | 88.6 |
| MS.gene81921.t1 | MTR_7g082390 | 26.562 | 256 | 163 | 7 | 28 | 277 | 10 | 246 | 4.46e-19 | 85.1 |
| MS.gene81921.t1 | MTR_7g082530 | 25.506 | 247 | 159 | 8 | 46 | 282 | 30 | 261 | 2.80e-17 | 82.4 |
| MS.gene81921.t1 | MTR_7g082530 | 25.506 | 247 | 159 | 8 | 46 | 282 | 30 | 261 | 2.97e-17 | 82.4 |
| MS.gene81921.t1 | MTR_6g082980 | 28.095 | 210 | 123 | 8 | 60 | 256 | 47 | 241 | 2.14e-13 | 69.3 |
| MS.gene81921.t1 | MTR_6g082850 | 25.541 | 231 | 140 | 9 | 56 | 273 | 37 | 248 | 1.59e-12 | 66.6 |
| MS.gene81921.t1 | MTR_6g082990 | 26.172 | 256 | 155 | 11 | 37 | 275 | 8 | 246 | 1.83e-12 | 66.6 |
| MS.gene81921.t1 | MTR_6g083020 | 25.306 | 245 | 148 | 10 | 56 | 285 | 41 | 265 | 1.01e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81921.t1 | AT1G66930 | 29.536 | 237 | 144 | 9 | 44 | 267 | 240 | 466 | 6.46e-16 | 78.6 |
| MS.gene81921.t1 | AT1G66930 | 29.237 | 236 | 144 | 9 | 44 | 266 | 59 | 284 | 1.22e-15 | 77.8 |
| MS.gene81921.t1 | AT1G66930 | 29.237 | 236 | 144 | 9 | 44 | 266 | 59 | 284 | 1.53e-15 | 77.4 |
| MS.gene81921.t1 | AT1G18390 | 26.991 | 226 | 139 | 8 | 56 | 271 | 33 | 242 | 4.16e-15 | 76.3 |
| MS.gene81921.t1 | AT5G38260 | 26.996 | 263 | 147 | 12 | 24 | 267 | 11 | 247 | 1.96e-14 | 73.9 |
| MS.gene81921.t1 | AT1G67000 | 31.081 | 222 | 130 | 10 | 58 | 267 | 256 | 466 | 4.90e-14 | 73.2 |
| MS.gene81921.t1 | AT1G66880 | 29.907 | 214 | 116 | 10 | 57 | 261 | 33 | 221 | 2.29e-12 | 67.8 |
| MS.gene81921.t1 | AT1G66880 | 29.907 | 214 | 116 | 10 | 57 | 261 | 33 | 221 | 2.96e-12 | 67.8 |
| MS.gene81921.t1 | AT1G66880 | 24.701 | 251 | 147 | 10 | 36 | 267 | 239 | 466 | 9.86e-11 | 63.2 |
| MS.gene81921.t1 | AT1G66880 | 29.048 | 210 | 116 | 8 | 60 | 261 | 416 | 600 | 3.77e-12 | 67.4 |
| MS.gene81921.t1 | AT1G66880 | 25.551 | 227 | 134 | 9 | 60 | 267 | 635 | 845 | 9.02e-11 | 63.2 |
| MS.gene81921.t1 | AT5G38210 | 23.077 | 221 | 148 | 6 | 57 | 267 | 20 | 228 | 3.15e-11 | 64.3 |
| MS.gene81921.t1 | AT5G38210 | 23.077 | 221 | 148 | 6 | 57 | 267 | 38 | 246 | 3.27e-11 | 64.3 |
| MS.gene81921.t1 | AT1G66910 | 24.335 | 263 | 156 | 9 | 57 | 288 | 38 | 288 | 6.76e-11 | 63.5 |
Find 56 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATTGTAAGTGACGATTTA+TGG | 0.177180 | 6.4:-56009113 | MS.gene81921:CDS |
| GCGATATTAGCTACCCATTC+TGG | 0.203275 | 6.4:-56009269 | MS.gene81921:CDS |
| ATTGGTTGATTTCAATGTTA+TGG | 0.260052 | 6.4:-56008863 | MS.gene81921:CDS |
| TATGAAGGATGTGAAACTTT+TGG | 0.265817 | 6.4:+56009160 | None:intergenic |
| CCTAGTTTCAAGTGACAAAT+TGG | 0.292169 | 6.4:-56008881 | MS.gene81921:CDS |
| ACCACATCTTGTGAACCTTT+TGG | 0.312693 | 6.4:-56009306 | MS.gene81921:CDS |
| TTCAGTTATGTGTCTCTTGC+CGG | 0.319841 | 6.4:-56008677 | MS.gene81921:CDS |
| AACGTAGAGGAGTTTGAGTT+TGG | 0.343075 | 6.4:+56008964 | None:intergenic |
| TAGGTGAAGGATTTGAGGTT+GGG | 0.354930 | 6.4:-56008777 | MS.gene81921:CDS |
| AAGAATAGTATCACATTTCC+TGG | 0.356109 | 6.4:-56008835 | MS.gene81921:CDS |
| TCGCTTTGGAGCGTTCTTCT+CGG | 0.377807 | 6.4:-56009443 | MS.gene81921:CDS |
| TGAGGTTGGGTGGAGTGGTG+TGG | 0.400616 | 6.4:-56008764 | MS.gene81921:CDS |
| GAGGTTGGGTGGAGTGGTGT+GGG | 0.406803 | 6.4:-56008763 | MS.gene81921:CDS |
| GAGACATTTGATTTATATGA+AGG | 0.409674 | 6.4:+56009145 | None:intergenic |
| TGACAATCAAGCTTGAAGTT+TGG | 0.421038 | 6.4:+56009213 | None:intergenic |
| GTCAATGAATTCTTCAAGCC+AGG | 0.429363 | 6.4:+56008817 | None:intergenic |
| TGAAGTGATTTCTTGCCTCA+TGG | 0.433114 | 6.4:-56008926 | MS.gene81921:CDS |
| GGATTTGAGGTTGGGTGGAG+TGG | 0.435864 | 6.4:-56008769 | MS.gene81921:CDS |
| AAAGAAAACGAAGGTAGAAA+AGG | 0.443625 | 6.4:+56009408 | None:intergenic |
| CCAATTTGTCACTTGAAACT+AGG | 0.446574 | 6.4:+56008881 | None:intergenic |
| TTTACATCAATATATTGCTT+CGG | 0.447673 | 6.4:+56009060 | None:intergenic |
| TTTGGATGGCCGCAATAACT+TGG | 0.464631 | 6.4:+56009231 | None:intergenic |
| GACTTAAGAAGAAGAGTGGA+AGG | 0.485625 | 6.4:+56009364 | None:intergenic |
| CTGAAATTTCCACATCCAAA+AGG | 0.487786 | 6.4:+56009291 | None:intergenic |
| TTAGGTGAAGGATTTGAGGT+TGG | 0.490349 | 6.4:-56008778 | MS.gene81921:CDS |
| AGAAACTAGGAACATCGCTT+TGG | 0.491158 | 6.4:-56009457 | MS.gene81921:CDS |
| GATGGCCGCAATAACTTGGT+TGG | 0.501772 | 6.4:+56009235 | None:intergenic |
| GTGAAGGATTTGAGGTTGGG+TGG | 0.502660 | 6.4:-56008774 | MS.gene81921:CDS |
| AATTGACTTAAGAAGAAGAG+TGG | 0.504168 | 6.4:+56009360 | None:intergenic |
| TGTGGTGGTTGCATGAAATC+TGG | 0.506044 | 6.4:-56008730 | MS.gene81921:CDS |
| CCATCTGCCATGGATTTCTC+CGG | 0.525776 | 6.4:+56008658 | None:intergenic |
| GATGGAGTAACATCATCAAA+TGG | 0.529485 | 6.4:-56008640 | MS.gene81921:CDS |
| AATCAAGCTTGAAGTTTGGA+TGG | 0.541494 | 6.4:+56009217 | None:intergenic |
| GGGAAATGCTAGAAGAAACT+AGG | 0.548819 | 6.4:-56009470 | None:intergenic |
| CCGGAGAAATCCATGGCAGA+TGG | 0.557417 | 6.4:-56008658 | MS.gene81921:CDS |
| GGATGTTAAGACTCCAGAAT+GGG | 0.565498 | 6.4:+56009256 | None:intergenic |
| AACGAAGGTAGAAAAGGAAG+CGG | 0.568895 | 6.4:+56009414 | None:intergenic |
| TCCAAAAGGTTCACAAGATG+TGG | 0.579964 | 6.4:+56009305 | None:intergenic |
| TTCAAGCTTGATTGTCAACA+TGG | 0.587950 | 6.4:-56009207 | MS.gene81921:CDS |
| GTGATTTCTTGCCTCATGGA+GGG | 0.587986 | 6.4:-56008922 | MS.gene81921:CDS |
| TGATGTTACTCCATCTGCCA+TGG | 0.590101 | 6.4:+56008648 | None:intergenic |
| AACATCCAACCAAGTTATTG+CGG | 0.590230 | 6.4:-56009240 | MS.gene81921:CDS |
| TGGATGTTAAGACTCCAGAA+TGG | 0.599363 | 6.4:+56009255 | None:intergenic |
| TCTCTTGCCGGAGAAATCCA+TGG | 0.611162 | 6.4:-56008665 | MS.gene81921:CDS |
| AAAAGGTTCACAAGATGTGG+TGG | 0.620771 | 6.4:+56009308 | None:intergenic |
| GAATTCATTGACAAATGACT+CGG | 0.637714 | 6.4:-56008806 | MS.gene81921:CDS |
| AGTGATTTCTTGCCTCATGG+AGG | 0.643094 | 6.4:-56008923 | MS.gene81921:CDS |
| ATGAAATCTGGTAGGAAATG+CGG | 0.644240 | 6.4:-56008718 | MS.gene81921:CDS |
| GAAGGTAGAAAAGGAAGCGG+AGG | 0.652700 | 6.4:+56009417 | None:intergenic |
| TATACTCTATTGTACGAGTG+TGG | 0.664109 | 6.4:-56008994 | MS.gene81921:CDS |
| AACTTCAGAACTCAACGTAG+AGG | 0.677319 | 6.4:+56008951 | None:intergenic |
| GTGGTTGCATGAAATCTGGT+AGG | 0.685356 | 6.4:-56008726 | MS.gene81921:CDS |
| GGTGGACACAGCATTAGTAG+TGG | 0.686937 | 6.4:+56009326 | None:intergenic |
| GGTGTGGGAGAAGAAATATG+TGG | 0.752804 | 6.4:-56008748 | MS.gene81921:CDS |
| GTGGGAGAAGAAATATGTGG+TGG | 0.753774 | 6.4:-56008745 | MS.gene81921:CDS |
| TTGATGATGAAAGAAAACGA+AGG | 0.776756 | 6.4:+56009399 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTACATCAATATATTGCTT+CGG | + | chr6.4:56008975-56008994 | None:intergenic | 20.0% |
| !! | ATTGGTTGATTTCAATGTTA+TGG | - | chr6.4:56009169-56009188 | MS.gene81921:CDS | 25.0% |
| !! | GAGACATTTGATTTATATGA+AGG | + | chr6.4:56008890-56008909 | None:intergenic | 25.0% |
| AAAGAAAACGAAGGTAGAAA+AGG | + | chr6.4:56008627-56008646 | None:intergenic | 30.0% | |
| AAGAATAGTATCACATTTCC+TGG | - | chr6.4:56009197-56009216 | MS.gene81921:CDS | 30.0% | |
| GAATTCATTGACAAATGACT+CGG | - | chr6.4:56009226-56009245 | MS.gene81921:CDS | 30.0% | |
| ! | AATTGACTTAAGAAGAAGAG+TGG | + | chr6.4:56008675-56008694 | None:intergenic | 30.0% |
| ! | ATCAAATGTCTCAACTTTTG+AGG | - | chr6.4:56008898-56008917 | MS.gene81921:CDS | 30.0% |
| ! | ATGAAGGATGTGAAACTTTT+GGG | + | chr6.4:56008874-56008893 | None:intergenic | 30.0% |
| ! | TATGAAGGATGTGAAACTTT+TGG | + | chr6.4:56008875-56008894 | None:intergenic | 30.0% |
| !! | TTGATGATGAAAGAAAACGA+AGG | + | chr6.4:56008636-56008655 | None:intergenic | 30.0% |
| AACATCCAACCAAGTTATTG+CGG | - | chr6.4:56008792-56008811 | MS.gene81921:CDS | 35.0% | |
| AATCAAGCTTGAAGTTTGGA+TGG | + | chr6.4:56008818-56008837 | None:intergenic | 35.0% | |
| ATGAAATCTGGTAGGAAATG+CGG | - | chr6.4:56009314-56009333 | MS.gene81921:CDS | 35.0% | |
| CTGAAATTTCCACATCCAAA+AGG | + | chr6.4:56008744-56008763 | None:intergenic | 35.0% | |
| GATGGAGTAACATCATCAAA+TGG | - | chr6.4:56009392-56009411 | MS.gene81921:CDS | 35.0% | |
| GGATTGTAAGTGACGATTTA+TGG | - | chr6.4:56008919-56008938 | MS.gene81921:CDS | 35.0% | |
| TATACTCTATTGTACGAGTG+TGG | - | chr6.4:56009038-56009057 | MS.gene81921:CDS | 35.0% | |
| TGACAATCAAGCTTGAAGTT+TGG | + | chr6.4:56008822-56008841 | None:intergenic | 35.0% | |
| TTCAAGCTTGATTGTCAACA+TGG | - | chr6.4:56008825-56008844 | MS.gene81921:CDS | 35.0% | |
| ! | CCAATTTGTCACTTGAAACT+AGG | + | chr6.4:56009154-56009173 | None:intergenic | 35.0% |
| ! | CCTAGTTTCAAGTGACAAAT+TGG | - | chr6.4:56009151-56009170 | MS.gene81921:CDS | 35.0% |
| !! | ACAAATGACTCGGATGTTTT+AGG | - | chr6.4:56009236-56009255 | MS.gene81921:CDS | 35.0% |
| !! | TGTTTTAGGTGAAGGATTTG+AGG | - | chr6.4:56009250-56009269 | MS.gene81921:CDS | 35.0% |
| AAAAGGTTCACAAGATGTGG+TGG | + | chr6.4:56008727-56008746 | None:intergenic | 40.0% | |
| AACGAAGGTAGAAAAGGAAG+CGG | + | chr6.4:56008621-56008640 | None:intergenic | 40.0% | |
| AACTTCAGAACTCAACGTAG+AGG | + | chr6.4:56009084-56009103 | None:intergenic | 40.0% | |
| AGAAACTAGGAACATCGCTT+TGG | - | chr6.4:56008575-56008594 | MS.gene81921:CDS | 40.0% | |
| GACTTAAGAAGAAGAGTGGA+AGG | + | chr6.4:56008671-56008690 | None:intergenic | 40.0% | |
| GGATGTTAAGACTCCAGAAT+GGG | + | chr6.4:56008779-56008798 | None:intergenic | 40.0% | |
| GTCAATGAATTCTTCAAGCC+AGG | + | chr6.4:56009218-56009237 | None:intergenic | 40.0% | |
| TCCAAAAGGTTCACAAGATG+TGG | + | chr6.4:56008730-56008749 | None:intergenic | 40.0% | |
| TGAAGTGATTTCTTGCCTCA+TGG | - | chr6.4:56009106-56009125 | MS.gene81921:CDS | 40.0% | |
| TGGATGTTAAGACTCCAGAA+TGG | + | chr6.4:56008780-56008799 | None:intergenic | 40.0% | |
| TTCAGTTATGTGTCTCTTGC+CGG | - | chr6.4:56009355-56009374 | MS.gene81921:CDS | 40.0% | |
| ! | AACGTAGAGGAGTTTGAGTT+TGG | + | chr6.4:56009071-56009090 | None:intergenic | 40.0% |
| ! | ACCACATCTTGTGAACCTTT+TGG | - | chr6.4:56008726-56008745 | MS.gene81921:CDS | 40.0% |
| ! | TAGGTGAAGGATTTGAGGTT+GGG | - | chr6.4:56009255-56009274 | MS.gene81921:CDS | 40.0% |
| ! | TCTTGTGAACCTTTTGGATG+TGG | - | chr6.4:56008732-56008751 | MS.gene81921:CDS | 40.0% |
| ! | TTAGGTGAAGGATTTGAGGT+TGG | - | chr6.4:56009254-56009273 | MS.gene81921:CDS | 40.0% |
| AGTGATTTCTTGCCTCATGG+AGG | - | chr6.4:56009109-56009128 | MS.gene81921:CDS | 45.0% | |
| GCGATATTAGCTACCCATTC+TGG | - | chr6.4:56008763-56008782 | MS.gene81921:CDS | 45.0% | |
| GGTGTGGGAGAAGAAATATG+TGG | - | chr6.4:56009284-56009303 | MS.gene81921:CDS | 45.0% | |
| GTGATTTCTTGCCTCATGGA+GGG | - | chr6.4:56009110-56009129 | MS.gene81921:CDS | 45.0% | |
| GTGGGAGAAGAAATATGTGG+TGG | - | chr6.4:56009287-56009306 | MS.gene81921:CDS | 45.0% | |
| GTGGTTGCATGAAATCTGGT+AGG | - | chr6.4:56009306-56009325 | MS.gene81921:CDS | 45.0% | |
| TGATGTTACTCCATCTGCCA+TGG | + | chr6.4:56009387-56009406 | None:intergenic | 45.0% | |
| TGTGGTGGTTGCATGAAATC+TGG | - | chr6.4:56009302-56009321 | MS.gene81921:CDS | 45.0% | |
| TTTGGATGGCCGCAATAACT+TGG | + | chr6.4:56008804-56008823 | None:intergenic | 45.0% | |
| !! | GACTCGGATGTTTTAGGTGA+AGG | - | chr6.4:56009242-56009261 | MS.gene81921:CDS | 45.0% |
| CCATCTGCCATGGATTTCTC+CGG | + | chr6.4:56009377-56009396 | None:intergenic | 50.0% | |
| GAAGGTAGAAAAGGAAGCGG+AGG | + | chr6.4:56008618-56008637 | None:intergenic | 50.0% | |
| GATGGCCGCAATAACTTGGT+TGG | + | chr6.4:56008800-56008819 | None:intergenic | 50.0% | |
| GGTGGACACAGCATTAGTAG+TGG | + | chr6.4:56008709-56008728 | None:intergenic | 50.0% | |
| TCTCTTGCCGGAGAAATCCA+TGG | - | chr6.4:56009367-56009386 | MS.gene81921:CDS | 50.0% | |
| ! | CACGAGTTTTACCCTCCATG+AGG | + | chr6.4:56009124-56009143 | None:intergenic | 50.0% |
| ! | GTGAAGGATTTGAGGTTGGG+TGG | - | chr6.4:56009258-56009277 | MS.gene81921:CDS | 50.0% |
| !! | TCGCTTTGGAGCGTTCTTCT+CGG | - | chr6.4:56008589-56008608 | MS.gene81921:CDS | 50.0% |
| CCGGAGAAATCCATGGCAGA+TGG | - | chr6.4:56009374-56009393 | MS.gene81921:CDS | 55.0% | |
| ! | GGATTTGAGGTTGGGTGGAG+TGG | - | chr6.4:56009263-56009282 | MS.gene81921:CDS | 55.0% |
| GAGGTTGGGTGGAGTGGTGT+GGG | - | chr6.4:56009269-56009288 | MS.gene81921:CDS | 60.0% | |
| TGAGGTTGGGTGGAGTGGTG+TGG | - | chr6.4:56009268-56009287 | MS.gene81921:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 56008567 | 56009487 | 56008567 | ID=MS.gene81921 |
| chr6.4 | mRNA | 56008567 | 56009487 | 56008567 | ID=MS.gene81921.t1;Parent=MS.gene81921 |
| chr6.4 | exon | 56008567 | 56009487 | 56008567 | ID=MS.gene81921.t1.exon1;Parent=MS.gene81921.t1 |
| chr6.4 | CDS | 56008567 | 56009487 | 56008567 | ID=cds.MS.gene81921.t1;Parent=MS.gene81921.t1 |
| Gene Sequence |
| Protein sequence |
