AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81961

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81961.t1 MTR_3g090130 92.827 474 25 2 1 474 1 465 0.0 905
MS.gene81961.t1 MTR_5g072570 80.297 472 89 1 1 472 1 468 0.0 803
MS.gene81961.t1 MTR_3g090150 79.536 474 91 3 1 472 1 470 0.0 788
MS.gene81961.t1 MTR_7g021130 47.005 434 221 6 34 465 31 457 9.53e-141 412
MS.gene81961.t1 MTR_3g434850 45.395 456 237 6 12 465 9 454 3.75e-140 410
MS.gene81961.t1 MTR_3g498755 46.119 438 227 6 32 465 13 445 1.53e-139 409
MS.gene81961.t1 MTR_8g099930 46.804 438 226 5 32 467 32 464 1.58e-139 409
MS.gene81961.t1 MTR_2g449850 45.183 436 226 6 35 467 39 464 8.99e-130 384
MS.gene81961.t1 MTR_3g079590 47.005 434 207 9 30 461 74 486 2.24e-124 371
MS.gene81961.t1 MTR_3g079570 46.469 439 209 10 30 465 75 490 1.92e-122 367
MS.gene81961.t1 MTR_7g013790 41.860 473 254 7 6 469 8 468 8.59e-121 361
MS.gene81961.t1 MTR_3g079560 46.085 447 212 11 26 467 70 492 4.79e-120 360
MS.gene81961.t1 MTR_1g040370 42.342 444 205 7 24 465 21 415 2.01e-119 356
MS.gene81961.t1 MTR_3g034290 42.247 445 239 6 26 469 67 494 5.10e-119 358
MS.gene81961.t1 MTR_3g079600 44.543 449 226 11 26 471 68 496 2.29e-118 356
MS.gene81961.t1 MTR_4g075150 41.684 475 251 11 1 465 3 461 7.57e-117 351
MS.gene81961.t1 MTR_4g075150 41.684 475 253 11 1 465 3 463 8.98e-117 351
MS.gene81961.t1 MTR_4g089155 42.257 452 239 8 27 467 47 487 1.01e-116 352
MS.gene81961.t1 MTR_0003s0560 42.630 441 233 7 26 465 66 487 2.28e-116 351
MS.gene81961.t1 MTR_3g079620 43.833 454 218 12 30 471 74 502 2.55e-116 351
MS.gene81961.t1 MTR_1g095560 41.139 474 258 9 1 468 1 459 4.41e-115 346
MS.gene81961.t1 MTR_2g012860 42.511 454 239 9 22 466 34 474 4.03e-114 344
MS.gene81961.t1 MTR_3g079630 43.860 456 216 12 30 471 74 503 4.62e-113 343
MS.gene81961.t1 MTR_1g021010 44.394 437 214 11 34 466 76 487 5.67e-113 342
MS.gene81961.t1 MTR_2g012850 41.256 446 242 7 31 467 33 467 1.74e-112 340
MS.gene81961.t1 MTR_1g021020 44.419 439 214 11 32 466 76 488 3.94e-111 337
MS.gene81961.t1 MTR_1g020090 44.218 441 210 11 31 465 87 497 4.06e-111 338
MS.gene81961.t1 MTR_2g062990 40.741 432 241 8 37 465 43 462 1.99e-109 332
MS.gene81961.t1 MTR_2g063000 40.092 434 243 8 37 465 42 463 1.38e-108 330
MS.gene81961.t1 MTR_2g081620 39.636 439 250 8 37 472 44 470 2.11e-108 330
MS.gene81961.t1 MTR_1g095560 38.538 506 258 10 1 468 1 491 2.69e-108 330
MS.gene81961.t1 MTR_4g122950 38.771 472 249 11 21 469 27 481 1.11e-107 328
MS.gene81961.t1 MTR_1g095540 40.638 470 254 11 6 467 6 458 1.55e-106 324
MS.gene81961.t1 MTR_2g062980 39.723 433 244 9 37 465 43 462 8.72e-106 323
MS.gene81961.t1 MTR_4g122960 39.530 468 249 11 20 468 26 478 3.82e-105 322
MS.gene81961.t1 MTR_1g102130 39.955 443 226 10 32 470 90 496 1.05e-99 308
MS.gene81961.t1 MTR_3g034290 42.254 355 191 5 26 379 67 408 1.98e-94 291
MS.gene81961.t1 MTR_2g063000 35.499 431 236 8 37 465 42 432 7.42e-87 273
MS.gene81961.t1 MTR_0003s0560 46.992 266 132 4 26 290 66 323 1.28e-79 251
MS.gene81961.t1 MTR_7g051430 32.186 494 270 13 18 461 14 492 8.88e-73 238
MS.gene81961.t1 MTR_8g011350 31.677 483 270 12 29 464 26 495 4.80e-71 234
MS.gene81961.t1 MTR_7g080180 30.769 507 287 14 10 464 4 498 6.79e-71 233
MS.gene81961.t1 MTR_0075s0010 33.504 391 184 9 30 413 4 325 8.43e-63 207
MS.gene81961.t1 MTR_8g011350 31.140 456 254 12 29 437 26 468 1.97e-62 211
MS.gene81961.t1 MTR_7g080180 29.892 465 262 14 10 422 4 456 1.69e-58 200
MS.gene81961.t1 MTR_7g051430 37.762 286 164 7 18 297 14 291 1.57e-51 180
MS.gene81961.t1 MTR_2g018630 29.754 447 282 13 33 464 36 465 3.98e-51 180
MS.gene81961.t1 MTR_2g018600 28.477 453 287 13 33 464 33 469 1.26e-49 176
MS.gene81961.t1 MTR_4g021490 28.636 440 286 13 34 459 40 465 3.53e-49 175
MS.gene81961.t1 MTR_4g021510 29.888 445 274 15 34 459 39 464 5.01e-48 172
MS.gene81961.t1 MTR_2g018590 28.571 469 297 14 18 464 17 469 1.29e-47 171
MS.gene81961.t1 MTR_4g021520 28.635 447 277 16 34 459 40 465 1.44e-47 171
MS.gene81961.t1 MTR_4g021535 28.184 479 292 19 4 459 12 461 4.71e-47 169
MS.gene81961.t1 MTR_4g021530 26.846 447 285 16 34 459 40 465 1.56e-45 165
MS.gene81961.t1 MTR_1g050545 29.907 428 261 11 45 461 81 480 1.15e-44 163
MS.gene81961.t1 MTR_4g021500 27.602 442 286 15 34 458 40 464 5.59e-44 166
MS.gene81961.t1 MTR_4g021500 27.945 433 267 16 40 459 482 882 3.76e-40 154
MS.gene81961.t1 MTR_4g021500 27.602 442 286 15 34 458 40 464 6.72e-44 166
MS.gene81961.t1 MTR_4g021500 28.345 441 273 15 40 459 482 900 2.31e-41 158
MS.gene81961.t1 MTR_4g057585 28.372 430 277 13 39 461 87 492 1.56e-42 157
MS.gene81961.t1 MTR_4g057595 28.504 421 271 12 47 461 97 493 9.06e-42 155
MS.gene81961.t1 MTR_3g100420 27.729 458 293 14 32 464 52 496 1.19e-41 155
MS.gene81961.t1 MTR_2g095750 27.064 436 271 12 44 464 97 500 4.36e-41 154
MS.gene81961.t1 MTR_4g057570 28.074 431 270 13 45 462 87 490 1.59e-40 152
MS.gene81961.t1 MTR_1g094155 28.700 446 268 11 26 461 81 486 1.40e-39 149
MS.gene81961.t1 MTR_2g095730 27.354 446 278 11 27 462 86 495 7.89e-39 147
MS.gene81961.t1 MTR_4g021520 28.293 410 252 16 34 422 40 428 2.37e-37 142
MS.gene81961.t1 MTR_4g057600 26.241 423 284 11 45 462 93 492 2.53e-36 140
MS.gene81961.t1 MTR_3g045770 24.594 431 304 10 33 454 26 444 4.42e-36 139
MS.gene81961.t1 MTR_4g021535 25.949 474 254 19 9 459 15 414 2.81e-34 133
MS.gene81961.t1 MTR_2g018630 28.291 357 231 10 117 464 20 360 8.52e-34 130
MS.gene81961.t1 MTR_5g097180 26.464 427 271 15 49 464 43 437 1.19e-33 132
MS.gene81961.t1 MTR_4g084710 25.277 451 288 15 41 474 37 455 1.71e-33 132
MS.gene81961.t1 MTR_2g009200 25.166 453 272 17 32 460 26 435 1.57e-30 123
MS.gene81961.t1 MTR_8g007520 78.571 70 15 0 350 419 111 180 5.39e-30 115
MS.gene81961.t1 MTR_2g095710 25.057 439 277 14 38 464 85 483 4.27e-25 108
MS.gene81961.t1 MTR_4g056520 23.571 420 277 12 65 460 55 454 4.16e-22 99.0
MS.gene81961.t1 MTR_1g051175 48.352 91 44 2 41 130 20 108 5.51e-19 87.4
MS.gene81961.t1 MTR_7g100560 27.488 211 87 3 40 250 65 209 4.35e-17 82.4
MS.gene81961.t1 MTR_6g086380 66.667 45 15 0 424 468 56 100 7.99e-13 65.1
MS.gene81961.t1 MTR_4g122970 40.000 80 41 4 4 80 12 87 3.34e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81961.t1 AT5G42240 74.000 450 117 0 25 474 24 473 0.0 711
MS.gene81961.t1 AT1G43780 70.536 448 131 1 26 472 30 477 0.0 696
MS.gene81961.t1 AT5G42230 68.293 451 143 0 18 468 13 463 0.0 664
MS.gene81961.t1 AT5G42230 68.293 451 143 0 18 468 13 463 0.0 664
MS.gene81961.t1 AT2G12480 67.888 464 138 4 12 472 7 462 0.0 662
MS.gene81961.t1 AT2G12480 64.655 464 129 5 12 472 7 438 0.0 618
MS.gene81961.t1 AT2G12480 64.224 464 129 5 12 472 7 436 0.0 613
MS.gene81961.t1 AT2G12480 64.086 465 129 6 12 472 7 437 0.0 608
MS.gene81961.t1 AT2G33530 48.741 437 217 5 32 466 32 463 2.50e-148 431
MS.gene81961.t1 AT1G28110 47.597 437 220 6 32 466 30 459 1.81e-141 414
MS.gene81961.t1 AT1G28110 47.597 437 220 6 32 466 30 459 1.81e-141 414
MS.gene81961.t1 AT4G30610 43.424 479 244 10 1 467 1 464 4.79e-122 364
MS.gene81961.t1 AT3G02110 41.139 474 251 8 6 465 12 471 4.22e-119 357
MS.gene81961.t1 AT2G24000 43.820 445 227 8 31 464 28 460 1.63e-118 355
MS.gene81961.t1 AT3G63470 42.105 456 242 9 17 469 65 501 2.13e-118 356
MS.gene81961.t1 AT5G23210 42.275 466 217 12 32 465 50 495 9.33e-117 352
MS.gene81961.t1 AT2G24010 43.052 439 235 7 31 467 28 453 1.56e-115 347
MS.gene81961.t1 AT4G30810 40.899 467 261 7 2 464 6 461 1.49e-114 346
MS.gene81961.t1 AT2G35780 42.727 440 232 9 31 465 27 451 1.41e-113 342
MS.gene81961.t1 AT5G08260 40.260 462 247 8 22 466 28 477 7.12e-113 342
MS.gene81961.t1 AT1G61130 42.438 443 238 10 30 465 28 460 1.90e-112 340
MS.gene81961.t1 AT2G24010 41.410 454 236 8 31 467 28 468 2.76e-110 334
MS.gene81961.t1 AT1G11080 41.538 455 233 10 31 465 48 489 2.07e-109 333
MS.gene81961.t1 AT1G11080 41.538 455 233 10 31 465 48 489 2.36e-109 333
MS.gene81961.t1 AT3G07990 40.724 442 246 8 30 467 30 459 2.49e-109 332
MS.gene81961.t1 AT2G35770 40.879 455 246 10 19 467 23 460 9.16e-109 330
MS.gene81961.t1 AT4G15100 40.377 478 260 9 5 467 16 483 7.57e-106 323
MS.gene81961.t1 AT3G17180 39.956 458 236 10 32 467 37 477 2.55e-104 319
MS.gene81961.t1 AT3G52020 40.590 441 241 10 31 467 77 500 2.81e-104 320
MS.gene81961.t1 AT2G24010 39.623 477 233 11 33 467 1 464 1.30e-103 317
MS.gene81961.t1 AT2G05850 39.640 444 243 8 31 467 61 486 9.91e-103 316
MS.gene81961.t1 AT3G52000 38.462 442 246 9 31 467 61 481 5.45e-102 313
MS.gene81961.t1 AT3G52010 39.775 445 240 10 31 467 62 486 7.83e-100 308
MS.gene81961.t1 AT5G23210 41.190 420 196 11 32 419 50 450 2.24e-99 306
MS.gene81961.t1 AT5G23210 39.423 416 200 12 82 465 5 400 8.06e-91 282
MS.gene81961.t1 AT5G23210 39.320 412 198 12 86 465 8 399 1.99e-89 278
MS.gene81961.t1 AT3G17180 38.293 410 214 10 32 419 37 429 6.88e-84 265
MS.gene81961.t1 AT2G24010 40.110 364 193 6 33 379 1 356 2.04e-82 259
MS.gene81961.t1 AT4G12910 32.265 499 290 13 5 464 8 497 1.15e-74 243
MS.gene81961.t1 AT5G23210 37.705 366 177 11 86 419 8 354 6.83e-72 232
MS.gene81961.t1 AT3G25420 31.328 482 273 10 29 464 25 494 8.20e-68 225
MS.gene81961.t1 AT4G12910 31.510 457 265 13 5 422 8 455 2.24e-61 207
MS.gene81961.t1 AT3G56540 57.962 157 64 2 31 186 77 232 1.16e-56 189
MS.gene81961.t1 AT2G24000 36.545 301 169 7 174 464 109 397 4.75e-51 178
MS.gene81961.t1 AT2G24000 60.440 91 35 1 31 120 28 118 2.69e-27 113
MS.gene81961.t1 AT1G33540 28.062 449 294 13 33 464 28 464 7.60e-51 179
MS.gene81961.t1 AT1G33540 28.140 430 281 12 51 464 4 421 5.65e-50 176
MS.gene81961.t1 AT2G22920 30.176 454 253 16 33 464 24 435 8.41e-50 176
MS.gene81961.t1 AT5G09640 29.590 463 272 16 33 464 26 465 2.07e-48 173
MS.gene81961.t1 AT1G73300 28.829 444 270 13 33 464 32 441 4.63e-48 171
MS.gene81961.t1 AT1G73300 28.829 444 270 13 33 464 32 441 4.63e-48 171
MS.gene81961.t1 AT2G22990 29.054 444 271 14 33 464 22 433 6.05e-48 171
MS.gene81961.t1 AT1G73290 29.318 440 271 12 33 464 31 438 9.63e-48 171
MS.gene81961.t1 AT3G45010 28.972 428 280 12 39 462 92 499 5.03e-47 170
MS.gene81961.t1 AT2G23000 28.859 447 270 16 33 464 24 437 3.02e-46 166
MS.gene81961.t1 AT2G23000 28.859 447 270 16 33 464 24 437 3.02e-46 166
MS.gene81961.t1 AT5G36180 28.507 442 274 14 33 464 32 441 4.19e-46 166
MS.gene81961.t1 AT1G73280 27.891 441 278 12 33 464 32 441 1.27e-45 165
MS.gene81961.t1 AT2G22980 27.828 442 273 15 33 464 25 430 2.46e-45 164
MS.gene81961.t1 AT2G22970 29.279 444 268 15 33 464 24 433 3.08e-45 164
MS.gene81961.t1 AT3G12203 29.291 437 268 13 37 464 12 416 7.04e-45 162
MS.gene81961.t1 AT1G73310 27.169 438 285 11 33 464 32 441 1.01e-44 162
MS.gene81961.t1 AT3G12203 29.291 437 268 13 37 464 33 437 1.36e-44 162
MS.gene81961.t1 AT3G10450 28.475 446 269 14 33 464 28 437 1.53e-44 162
MS.gene81961.t1 AT1G73270 28.090 445 272 15 33 464 32 441 1.61e-44 162
MS.gene81961.t1 AT1G73270 28.090 445 272 15 33 464 32 441 2.04e-44 162
MS.gene81961.t1 AT3G12240 27.438 441 280 13 33 464 27 436 4.64e-44 160
MS.gene81961.t1 AT5G22980 28.276 435 280 11 44 473 95 502 6.18e-44 162
MS.gene81961.t1 AT3G10450 28.337 427 257 13 51 464 4 394 4.48e-43 157
MS.gene81961.t1 AT3G12230 27.664 441 279 14 33 464 26 435 4.91e-43 157
MS.gene81961.t1 AT2G22990 28.947 418 254 13 33 438 22 408 1.25e-42 156
MS.gene81961.t1 AT3G12203 29.440 411 250 12 62 464 1 379 1.73e-42 155
MS.gene81961.t1 AT2G23010 26.457 446 282 11 33 464 24 437 1.78e-42 156
MS.gene81961.t1 AT2G22990 28.878 419 255 13 33 439 22 409 1.80e-42 157
MS.gene81961.t1 AT3G12220 27.211 441 281 13 33 464 26 435 3.13e-42 155
MS.gene81961.t1 AT2G23010 26.233 446 283 11 33 464 24 437 7.49e-42 154
MS.gene81961.t1 AT2G22990 28.378 444 265 15 33 464 22 424 1.13e-41 154
MS.gene81961.t1 AT2G22980 27.818 417 256 14 33 439 25 406 2.89e-41 152
MS.gene81961.t1 AT2G22970 29.524 420 251 14 33 440 24 410 5.51e-41 152
MS.gene81961.t1 AT2G22980 27.885 416 255 14 33 438 25 405 8.12e-41 152
MS.gene81961.t1 AT2G22970 29.356 419 251 14 33 439 24 409 1.16e-40 150
MS.gene81961.t1 AT3G12220 27.251 422 268 12 51 464 15 405 1.67e-40 150
MS.gene81961.t1 AT2G22920 29.710 414 228 15 33 424 24 396 1.92e-40 150
MS.gene81961.t1 AT3G10410 28.276 435 284 13 39 468 90 501 3.12e-40 152
MS.gene81961.t1 AT3G10450 28.266 421 253 13 33 439 28 413 8.21e-40 149
MS.gene81961.t1 AT1G73290 28.250 400 248 11 33 424 31 399 2.76e-38 144
MS.gene81961.t1 AT3G10450 28.109 402 241 12 51 439 4 370 2.77e-38 143
MS.gene81961.t1 AT1G73310 26.379 417 270 11 33 439 32 421 6.77e-36 138
MS.gene81961.t1 AT1G15000 26.977 430 276 12 41 464 29 426 1.30e-33 132
MS.gene81961.t1 AT2G22920 35.268 224 134 5 33 248 24 244 3.48e-33 128
MS.gene81961.t1 AT1G73290 33.929 224 137 5 33 248 31 251 1.41e-31 123
MS.gene81961.t1 AT2G22970 34.821 224 134 5 33 248 24 243 2.10e-31 123
MS.gene81961.t1 AT1G73280 29.167 264 168 6 33 281 32 291 2.84e-30 121
MS.gene81961.t1 AT2G22990 27.143 350 218 11 121 464 1 319 1.34e-29 118
MS.gene81961.t1 AT2G22980 30.224 268 165 9 33 290 25 280 2.04e-29 118
MS.gene81961.t1 AT1G73280 25.641 351 228 9 117 464 19 339 2.21e-29 118
MS.gene81961.t1 AT5G36180 26.705 352 223 11 117 464 19 339 2.72e-29 118
MS.gene81961.t1 AT5G36180 26.705 352 223 11 117 464 19 339 2.72e-29 118
MS.gene81961.t1 AT2G22990 26.857 350 219 11 121 464 1 319 7.81e-29 116
MS.gene81961.t1 AT1G73280 25.291 344 224 9 124 464 1 314 3.07e-27 111
MS.gene81961.t1 AT3G12220 29.545 220 148 3 33 246 26 244 4.18e-25 107
MS.gene81961.t1 AT5G36180 25.959 339 217 10 105 439 7 315 1.48e-24 104
MS.gene81961.t1 AT2G22980 24.768 323 205 11 121 439 1 289 1.91e-23 100
MS.gene81961.t1 AT5G22960 32.000 175 97 4 39 212 25 178 3.70e-22 94.4
MS.gene81961.t1 AT1G73310 24.465 327 217 8 117 439 19 319 3.03e-21 94.7
MS.gene81961.t1 AT5G36180 26.282 312 196 10 117 424 19 300 3.96e-20 91.7
MS.gene81961.t1 AT2G27920 23.928 443 299 15 46 464 33 461 2.13e-18 87.8
MS.gene81961.t1 AT2G22920 27.458 295 157 13 186 464 3 256 3.63e-18 84.7
MS.gene81961.t1 AT2G22960 27.465 142 95 4 323 464 51 184 8.69e-11 61.6

Find 116 sgRNAs with CRISPR-Local

Find 208 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCAAAATCATATTCCAATA+TGG 0.199431 3.2:-76367453 MS.gene81961:CDS
TGGAAACCCACTTCTGAAAA+TGG 0.247519 3.2:-76368465 MS.gene81961:CDS
AGTTTCAAGTTCAACATAAA+AGG 0.261935 3.2:-76368658 MS.gene81961:CDS
ATATTCCAATATGGGTTTAT+AGG 0.279113 3.2:-76367444 MS.gene81961:intron
AGCTTGTTGGTAACAACAAT+TGG 0.326510 3.2:-76370318 MS.gene81961:CDS
TGCCATATGGACAACCTTCT+AGG 0.327165 3.2:-76367023 MS.gene81961:CDS
CCATCACCAGAAGGATAAAA+TGG 0.329912 3.2:+76369660 None:intergenic
ATGAAATTGGTCTTGCCATT+AGG 0.331349 3.2:-76368388 MS.gene81961:CDS
TTACAGACTCATATAGAGTT+TGG 0.337975 3.2:-76367248 MS.gene81961:CDS
TCGAGGAAGTTGTTTCTTAC+CGG 0.344513 3.2:-76369032 MS.gene81961:CDS
GATTCAGTTGTTCCATTACT+AGG 0.346899 3.2:-76367318 MS.gene81961:CDS
GGAGGTTGGGTAACTGAGTA+TGG 0.355586 3.2:-76367090 MS.gene81961:CDS
TGAACTATCCGTTTGAGAAT+TGG 0.357516 3.2:+76367472 None:intergenic
CATATACATACCTGTATTCC+AGG 0.358264 3.2:+76369611 None:intergenic
TCAAAATCATATTCCAATAT+GGG 0.358601 3.2:-76367452 MS.gene81961:CDS
TTCACAACTAGATCTTCAAT+TGG 0.361948 3.2:+76370282 None:intergenic
CTACACATGCCCCATGGATA+TGG 0.362091 3.2:+76367990 None:intergenic
AGTTTCCAATCTCCTCTTTG+TGG 0.370612 3.2:-76369328 MS.gene81961:CDS
AACTACCTATAAACCCATAT+TGG 0.374766 3.2:+76367439 None:intergenic
TACAATTCATTCTCCAATTG+AGG 0.378099 3.2:+76366942 None:intergenic
AAGCAACATATGAATATTAC+TGG 0.389075 3.2:-76368430 MS.gene81961:CDS
GTGTTGTTTGTGGTGGCATA+TGG 0.390969 3.2:-76370342 MS.gene81961:CDS
CTGGTCACACGGAATGATTT+CGG 0.395080 3.2:-76368411 MS.gene81961:CDS
ATTGAGGGTTTAGTAGTGTT+TGG 0.395143 3.2:+76366958 None:intergenic
GCCCTAGAAGGTTGTCCATA+TGG 0.398512 3.2:+76367021 None:intergenic
ATATATTTACCTGCCCTTTG+TGG 0.407790 3.2:+76367223 None:intergenic
CTCTTTGTGGAGTCTCCCGC+TGG 0.412475 3.2:-76369315 MS.gene81961:CDS
GGAATGATTTCGGATGAAAT+TGG 0.412496 3.2:-76368401 MS.gene81961:CDS
AATTCAAAGTCCTGGAATAC+AGG 0.414065 3.2:-76369621 MS.gene81961:intron
ATATTGACTGAATTTAACTT+TGG 0.416798 3.2:+76370245 None:intergenic
ATGGGAAAGTGTTGGTTTGT+TGG 0.421010 3.2:-76370366 MS.gene81961:CDS
TATTGACTGAATTTAACTTT+GGG 0.423688 3.2:+76370246 None:intergenic
GCTTGTTGGTAACAACAATT+GGG 0.424484 3.2:-76370317 MS.gene81961:CDS
ATTTCTTGTTCAGCTATAGC+TGG 0.425988 3.2:+76368227 None:intergenic
GTAACAACAATTGGGGTAGA+AGG 0.426136 3.2:-76370309 MS.gene81961:CDS
TATTACCTCCATTGAGCCAT+AGG 0.439967 3.2:+76370130 None:intergenic
AAAATCACAATCATTCCTAA+TGG 0.440186 3.2:+76368373 None:intergenic
AACACTACTAAACCCTCAAT+TGG 0.441749 3.2:-76366955 MS.gene81961:CDS
AACTTCCTCGATTTGTATGA+AGG 0.442362 3.2:+76369044 None:intergenic
GTAATGGAACAACTGAATCT+TGG 0.442814 3.2:+76367322 None:intergenic
TACGAAGAAATTCAAAGTCC+TGG 0.444786 3.2:-76369629 MS.gene81961:CDS
AATAGATGTAATGCCCTAGA+AGG 0.456374 3.2:+76367009 None:intergenic
GTTTGTTGGAGTGTTGTTTG+TGG 0.459216 3.2:-76370352 MS.gene81961:CDS
AAAAGCACCTCCTCCGATAG+AGG 0.462804 3.2:+76369695 None:intergenic
TGGAGAGCCTTCTGAACCTC+AGG 0.468727 3.2:+76368032 None:intergenic
GTGGAGTCTCCCGCTGGAGT+TGG 0.470449 3.2:-76369309 MS.gene81961:CDS
ATAGAGTTTGGTTCCACAAA+GGG 0.470528 3.2:-76367236 MS.gene81961:CDS
GCGGGTCACATGGTGCCATA+TGG 0.470975 3.2:-76367036 MS.gene81961:CDS
TACTAATCTGCCATATCCAT+GGG 0.479527 3.2:-76368000 MS.gene81961:CDS
GTTAAATTCAGTCAATATGC+TGG 0.485104 3.2:-76370240 MS.gene81961:CDS
TTCGCCACTGTAAGAGGAGC+GGG 0.485217 3.2:-76367054 MS.gene81961:CDS
CCTCTCACCCTATGGCTCAA+TGG 0.485243 3.2:-76370138 MS.gene81961:CDS
GACTGAATTTAACTTTGGGT+TGG 0.486607 3.2:+76370250 None:intergenic
ACAACTTCTGATTATAACAT+TGG 0.489905 3.2:-76369270 MS.gene81961:CDS
AGACTCCACAAAGAGGAGAT+TGG 0.491453 3.2:+76369323 None:intergenic
GATCTAGTTGTGAAGTTGCC+AGG 0.494140 3.2:-76370273 MS.gene81961:CDS
TGGACCTGCATAGCTTTCTC+CGG 0.497380 3.2:+76369013 None:intergenic
TATCCTTCTGGTGATGGACG+CGG 0.498312 3.2:-76369654 MS.gene81961:CDS
CAGGAACAAAAGCATGTCAT+TGG 0.499350 3.2:+76369088 None:intergenic
ACAATTCATTCTCCAATTGA+GGG 0.500289 3.2:+76366943 None:intergenic
AATTTAACTTTGGGTTGGCC+TGG 0.501987 3.2:+76370255 None:intergenic
ATATGCTTCCAATTCTCAAA+CGG 0.502822 3.2:-76367480 MS.gene81961:CDS
TGTTGGAGTGTTGTTTGTGG+TGG 0.503160 3.2:-76370349 MS.gene81961:CDS
GCCATATGGACAACCTTCTA+GGG 0.514262 3.2:-76367022 MS.gene81961:CDS
ATTATAATCAAGAATGGCAT+TGG 0.521613 3.2:+76368693 None:intergenic
CTATTCAGCTCCTTTGTGCA+TGG 0.523679 3.2:-76366991 MS.gene81961:CDS
TTATTAGAATGGGAAAGTGT+TGG 0.524595 3.2:-76370374 None:intergenic
CTTACCGGAGAAAGCTATGC+AGG 0.524600 3.2:-76369017 MS.gene81961:intron
TCTGAACCTCAGGCAGGTTG+AGG 0.529529 3.2:+76368042 None:intergenic
GGTCCGGGATGTTCCTCTAT+CGG 0.532762 3.2:-76369708 MS.gene81961:CDS
ATGAGCATTATAATCAAGAA+TGG 0.534017 3.2:+76368687 None:intergenic
AACATTGGTGACGCCTCCAC+AGG 0.534280 3.2:-76369255 MS.gene81961:intron
ATTACCTCCATTGAGCCATA+GGG 0.535044 3.2:+76370131 None:intergenic
TCACAACTAGATCTTCAATT+GGG 0.535707 3.2:+76370283 None:intergenic
GTACTAATCTGCCATATCCA+TGG 0.541288 3.2:-76368001 MS.gene81961:CDS
ACTTCTCGTACCATTTCAGC+AGG 0.548397 3.2:+76369069 None:intergenic
GGTGGCATATGGAAGCTTGT+TGG 0.550255 3.2:-76370331 MS.gene81961:CDS
ATACGTTATGAGTAGTTGCA+TGG 0.562834 3.2:+76368339 None:intergenic
GCAGATTAGTACGGTTGGCA+TGG 0.563219 3.2:+76368012 None:intergenic
AGAATGGCATTGGCTAATTG+AGG 0.563874 3.2:+76368703 None:intergenic
CAACCTGCCTGAGGTTCAGA+AGG 0.564507 3.2:-76368039 MS.gene81961:CDS
GAGCCTTCTGAACCTCAGGC+AGG 0.567516 3.2:+76368036 None:intergenic
AGTTTGGTTCCACAAAGGGC+AGG 0.573792 3.2:-76367232 MS.gene81961:intron
GGATGTTCCTCTATCGGAGG+AGG 0.577355 3.2:-76369702 MS.gene81961:CDS
TATGACCAACCAACTCCAGC+GGG 0.579633 3.2:+76369300 None:intergenic
ATATGGCAGATTAGTACGGT+TGG 0.580186 3.2:+76368007 None:intergenic
AGTCTCCCGCTGGAGTTGGT+TGG 0.582374 3.2:-76369305 MS.gene81961:CDS
ATGGATATGGCAGATTAGTA+CGG 0.582412 3.2:+76368003 None:intergenic
GCATGTGTAGGCTGGAGGTT+GGG 0.585017 3.2:-76367103 MS.gene81961:intron
ATTGGAAGCATATTAGCGTA+AGG 0.588336 3.2:+76367490 None:intergenic
TAACAACAATTGGGGTAGAA+GGG 0.589048 3.2:-76370308 MS.gene81961:CDS
TACGTTATGAGTAGTTGCAT+GGG 0.589405 3.2:+76368340 None:intergenic
TGCATGTGTAGGCTGGAGGT+TGG 0.590249 3.2:-76367104 MS.gene81961:intron
AGTGTTCGAGAACCTAGTAA+TGG 0.592866 3.2:+76367306 None:intergenic
CCATTGAGCCATAGGGTGAG+AGG 0.592895 3.2:+76370138 None:intergenic
GTTGATATAGATATAAAACA+TGG 0.595163 3.2:-76370213 MS.gene81961:CDS
TGTAAGAGGAGCGGGTCACA+TGG 0.600160 3.2:-76367046 MS.gene81961:CDS
TACTCACTACACATGCCCCA+TGG 0.601045 3.2:+76367984 None:intergenic
TATAGAGTTTGGTTCCACAA+AGG 0.604917 3.2:-76367237 MS.gene81961:CDS
AGTTTCCTTCATACAAATCG+AGG 0.605088 3.2:-76369049 MS.gene81961:CDS
TAATTACCTCAACCTGCCTG+AGG 0.606984 3.2:-76368048 MS.gene81961:CDS
GTGACCCGCTCCTCTTACAG+TGG 0.608929 3.2:+76367050 None:intergenic
CCTCCGATAGAGGAACATCC+CGG 0.613367 3.2:+76369705 None:intergenic
CAATCTCTTTCCATGCACAA+AGG 0.621559 3.2:+76366981 None:intergenic
TTGATATAGATATAAAACAT+GGG 0.633528 3.2:-76370212 MS.gene81961:CDS
TTTCGCCACTGTAAGAGGAG+CGG 0.634648 3.2:-76367055 MS.gene81961:CDS
CCGGGATGTTCCTCTATCGG+AGG 0.639230 3.2:-76369705 MS.gene81961:CDS
CTCACCCTATGGCTCAATGG+AGG 0.646306 3.2:-76370135 MS.gene81961:intron
TATGAATATTACTGGTCACA+CGG 0.646588 3.2:-76368422 MS.gene81961:CDS
CAGCGGGAGACTCCACAAAG+AGG 0.654135 3.2:+76369316 None:intergenic
AGACCGCGTCCATCACCAGA+AGG 0.655604 3.2:+76369651 None:intergenic
CTTGTTGGTAACAACAATTG+GGG 0.679916 3.2:-76370316 MS.gene81961:CDS
ATATAGATATAAAACATGGG+AGG 0.711528 3.2:-76370209 MS.gene81961:CDS
TTAATGCATGTGTAGGCTGG+AGG 0.712606 3.2:-76367108 MS.gene81961:intron
GTATGACCAACCAACTCCAG+CGG 0.719006 3.2:+76369299 None:intergenic
ACTAATCTGCCATATCCATG+GGG 0.764052 3.2:-76367999 MS.gene81961:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTATTTTATATATTTT+TGG - chr3.2:76369926-76369945 MS.gene81961:intron 0.0%
!! AAACGATAAATTATTAAATT+TGG + chr3.2:76368347-76368366 None:intergenic 10.0%
!! ATAAATAAATTGTTCTTTAT+TGG - chr3.2:76367552-76367571 MS.gene81961:intron 10.0%
!! CTTTGATATATAAATATTAT+TGG - chr3.2:76369510-76369529 MS.gene81961:intron 10.0%
!!! AATTTGTTAGTTTTATTATT+AGG - chr3.2:76368170-76368189 MS.gene81961:intron 10.0%
!! ACATCTTAAAATAAAGAAAA+AGG + chr3.2:76367474-76367493 None:intergenic 15.0%
!! ATTTAATATATCATGTGAAT+TGG + chr3.2:76368696-76368715 None:intergenic 15.0%
!! TCATAATAATTTAACAAGTA+TGG + chr3.2:76369963-76369982 None:intergenic 15.0%
!! TTGATATAGATATAAAACAT+GGG - chr3.2:76367102-76367121 MS.gene81961:intron 15.0%
!! TTGTTAAATTATTATGATAG+TGG - chr3.2:76369966-76369985 MS.gene81961:intron 15.0%
!! TTTCAATATGCAAAAAAATT+AGG + chr3.2:76370135-76370154 None:intergenic 15.0%
!!! ATTAACTTGATTAATTTTCT+AGG - chr3.2:76367585-76367604 MS.gene81961:intron 15.0%
!!! TATTTTTTGTATGTTATACA+TGG - chr3.2:76368147-76368166 MS.gene81961:intron 15.0%
!!! TTAAATTAATTTTGTCTACA+AGG + chr3.2:76369412-76369431 None:intergenic 15.0%
!! AAAAATTAAATTTAGTGTCG+TGG - chr3.2:76368376-76368395 MS.gene81961:CDS 20.0%
!! AATAAATTGTTCTTTATTGG+AGG - chr3.2:76367555-76367574 MS.gene81961:intron 20.0%
!! AATTTGATTATTGTGTTTGT+TGG + chr3.2:76368324-76368343 None:intergenic 20.0%
!! AGAGATATAATTGATATGAA+TGG - chr3.2:76369553-76369572 MS.gene81961:intron 20.0%
!! ATAAATTGTTCTTTATTGGA+GGG - chr3.2:76367556-76367575 MS.gene81961:intron 20.0%
!! ATAATTTAACAAGTATGGTA+AGG + chr3.2:76369958-76369977 None:intergenic 20.0%
!! CAACATACGAAATTAAATAA+AGG + chr3.2:76367274-76367293 None:intergenic 20.0%
!! GTTGATATAGATATAAAACA+TGG - chr3.2:76367101-76367120 MS.gene81961:intron 20.0%
!! TAAAAAATTAATGCATGTGT+AGG - chr3.2:76370199-76370218 MS.gene81961:CDS 20.0%
!! TCAAAATCATATTCCAATAT+GGG - chr3.2:76369862-76369881 MS.gene81961:intron 20.0%
!! TGAATGGAGTTTATAAATAT+AGG - chr3.2:76369569-76369588 MS.gene81961:intron 20.0%
!! TTCAAAATCATATTCCAATA+TGG - chr3.2:76369861-76369880 MS.gene81961:intron 20.0%
!! TTGATATTTGAGAATTATGT+GGG - chr3.2:76367916-76367935 MS.gene81961:intron 20.0%
!! TTTGATATTTGAGAATTATG+TGG - chr3.2:76367915-76367934 MS.gene81961:intron 20.0%
!! TTTGTTTATTTCATTAATGC+AGG - chr3.2:76369194-76369213 MS.gene81961:intron 20.0%
!!! ACGACACTAAATTTAATTTT+TGG + chr3.2:76368377-76368396 None:intergenic 20.0%
!!! AGATATTGACTACATAAATT+TGG + chr3.2:76368106-76368125 None:intergenic 20.0%
!!! ATATTGACTGAATTTAACTT+TGG + chr3.2:76367072-76367091 None:intergenic 20.0%
!!! ATGATATAATTTTGTTAAGC+TGG + chr3.2:76367421-76367440 None:intergenic 20.0%
!!! ATTGAAAATTGATTTGAGTA+TGG - chr3.2:76370148-76370167 MS.gene81961:CDS 20.0%
!!! TATTGACTGAATTTAACTTT+GGG + chr3.2:76367071-76367090 None:intergenic 20.0%
! AAAACCTATTTCATCATTTC+TGG - chr3.2:76368466-76368485 MS.gene81961:CDS 25.0%
! AAAATCACAATCATTCCTAA+TGG + chr3.2:76368944-76368963 None:intergenic 25.0%
! AAATATTCAATTTAGCAGAG+AGG - chr3.2:76368403-76368422 MS.gene81961:CDS 25.0%
! AAGCAACATATGAATATTAC+TGG - chr3.2:76368884-76368903 MS.gene81961:intron 25.0%
! ACAACTTCTGATTATAACAT+TGG - chr3.2:76368044-76368063 MS.gene81961:CDS 25.0%
! ACATGAACAATATATAAGTC+AGG + chr3.2:76367952-76367971 None:intergenic 25.0%
! AGAAATCAGATTGAAAAAGA+TGG - chr3.2:76369104-76369123 MS.gene81961:intron 25.0%
! AGATGATTTGAATTTGATGT+AGG + chr3.2:76367522-76367541 None:intergenic 25.0%
! ATATAGATATAAAACATGGG+AGG - chr3.2:76367105-76367124 MS.gene81961:intron 25.0%
! ATATTCCAATATGGGTTTAT+AGG - chr3.2:76369870-76369889 MS.gene81961:intron 25.0%
! ATGAGCATTATAATCAAGAA+TGG + chr3.2:76368630-76368649 None:intergenic 25.0%
! ATTATAATCAAGAATGGCAT+TGG + chr3.2:76368624-76368643 None:intergenic 25.0%
! CAAATTCAAATCATCTTACA+AGG - chr3.2:76367526-76367545 MS.gene81961:intron 25.0%
! TAAATTGTTCTTTATTGGAG+GGG - chr3.2:76367557-76367576 MS.gene81961:intron 25.0%
!! TCTTACATACTAACATTTTC+AGG - chr3.2:76368578-76368597 MS.gene81961:intron 25.0%
!! TTGTTATCATGAAGTTTTAC+CGG - chr3.2:76369377-76369396 MS.gene81961:intron 25.0%
!! TTTTATGTTGAACTTGAAAC+TGG + chr3.2:76368657-76368676 None:intergenic 25.0%
!!! AGTTTCAAGTTCAACATAAA+AGG - chr3.2:76368656-76368675 MS.gene81961:CDS 25.0%
!!! ATTTCGTATGTTGATAGTAT+AGG - chr3.2:76367281-76367300 MS.gene81961:CDS 25.0%
!!! TCTCTTCTATTATTTTGTTG+AGG - chr3.2:76367130-76367149 MS.gene81961:intron 25.0%
AACCATCAAATTTACTAGCT+AGG - chr3.2:76369639-76369658 MS.gene81961:CDS 30.0%
AACTACCTATAAACCCATAT+TGG + chr3.2:76369878-76369897 None:intergenic 30.0%
ACAAAGCAAATTTCAAGATC+AGG - chr3.2:76367809-76367828 MS.gene81961:intron 30.0%
ATATGCTTCCAATTCTCAAA+CGG - chr3.2:76369834-76369853 MS.gene81961:intron 30.0%
ATCATGTGAATTGGATCTTA+CGG + chr3.2:76368687-76368706 None:intergenic 30.0%
GTTAAATTCAGTCAATATGC+TGG - chr3.2:76367074-76367093 MS.gene81961:CDS 30.0%
TAGGCAAAAGAACACAATTA+AGG + chr3.2:76368533-76368552 None:intergenic 30.0%
TATGAATATTACTGGTCACA+CGG - chr3.2:76368892-76368911 MS.gene81961:intron 30.0%
TCACAACTAGATCTTCAATT+GGG + chr3.2:76367034-76367053 None:intergenic 30.0%
TTACAGACTCATATAGAGTT+TGG - chr3.2:76370066-76370085 MS.gene81961:intron 30.0%
TTCACAACTAGATCTTCAAT+TGG + chr3.2:76367035-76367054 None:intergenic 30.0%
TTCCAATCTACCAAAGTTAA+GGG + chr3.2:76368834-76368853 None:intergenic 30.0%
TTCCTAGCTAGTAAATTTGA+TGG + chr3.2:76369644-76369663 None:intergenic 30.0%
TTTCCAATCTACCAAAGTTA+AGG + chr3.2:76368835-76368854 None:intergenic 30.0%
! ACTACATGAAAGCAAGATTT+TGG + chr3.2:76367756-76367775 None:intergenic 30.0%
! CAAATCTAATTTTGTACCTG+TGG + chr3.2:76368078-76368097 None:intergenic 30.0%
! CTTGATTAATTTTCTAGGTC+CGG - chr3.2:76367590-76367609 MS.gene81961:intron 30.0%
! GAAATTTGCTTTGTCTAATC+TGG + chr3.2:76367803-76367822 None:intergenic 30.0%
! TGTTATCATGAAGTTTTACC+GGG - chr3.2:76369378-76369397 MS.gene81961:intron 30.0%
! TTGATTAATTTTCTAGGTCC+GGG - chr3.2:76367591-76367610 MS.gene81961:intron 30.0%
!! TACATGAGCTGTTATTTCTT+CGG + chr3.2:76369762-76369781 None:intergenic 30.0%
!! TCTTACTTTTCCCTTAACTT+TGG - chr3.2:76368821-76368840 MS.gene81961:intron 30.0%
!!! CTATATATTTGTGCCACTTT+TGG - chr3.2:76368730-76368749 MS.gene81961:intron 30.0%
AAATTAATGCATGTGTAGGC+TGG - chr3.2:76370203-76370222 MS.gene81961:CDS 35.0%
AACACTACTAAACCCTCAAT+TGG - chr3.2:76370359-76370378 MS.gene81961:CDS 35.0%
AATAGATGTAATGCCCTAGA+AGG + chr3.2:76370308-76370327 None:intergenic 35.0%
AATTCAAAGTCCTGGAATAC+AGG - chr3.2:76367693-76367712 MS.gene81961:intron 35.0%
AGGAAAAACTTGTGACAACA+AGG + chr3.2:76367254-76367273 None:intergenic 35.0%
AGTTTCCTTCATACAAATCG+AGG - chr3.2:76368265-76368284 MS.gene81961:CDS 35.0%
ATACGTTATGAGTAGTTGCA+TGG + chr3.2:76368978-76368997 None:intergenic 35.0%
ATATATTTACCTGCCCTTTG+TGG + chr3.2:76370094-76370113 None:intergenic 35.0%
ATGGATATGGCAGATTAGTA+CGG + chr3.2:76369314-76369333 None:intergenic 35.0%
ATTGAGGGTTTAGTAGTGTT+TGG + chr3.2:76370359-76370378 None:intergenic 35.0%
ATTTCTTGTTCAGCTATAGC+TGG + chr3.2:76369090-76369109 None:intergenic 35.0%
CATATACATACCTGTATTCC+AGG + chr3.2:76367706-76367725 None:intergenic 35.0%
GACAATAAAACCACCTGATT+TGG + chr3.2:76367378-76367397 None:intergenic 35.0%
GATTCAGTTGTTCCATTACT+AGG - chr3.2:76369996-76370015 MS.gene81961:intron 35.0%
GGAATGATTTCGGATGAAAT+TGG - chr3.2:76368913-76368932 MS.gene81961:intron 35.0%
GGCAGAACATAATAATCCAA+AGG + chr3.2:76369139-76369158 None:intergenic 35.0%
TAACAACAATTGGGGTAGAA+GGG - chr3.2:76367006-76367025 MS.gene81961:CDS 35.0%
TACGAAGAAATTCAAAGTCC+TGG - chr3.2:76367685-76367704 MS.gene81961:intron 35.0%
TACGTTATGAGTAGTTGCAT+GGG + chr3.2:76368977-76368996 None:intergenic 35.0%
TACTAATCTGCCATATCCAT+GGG - chr3.2:76369314-76369333 MS.gene81961:CDS 35.0%
TCTGCCAGAAATGATGAAAT+AGG + chr3.2:76368473-76368492 None:intergenic 35.0%
TGAACTATCCGTTTGAGAAT+TGG + chr3.2:76369845-76369864 None:intergenic 35.0%
TTCCCTTAACTTTGGTAGAT+TGG - chr3.2:76368829-76368848 MS.gene81961:intron 35.0%
! AACTTCCTCGATTTGTATGA+AGG + chr3.2:76368273-76368292 None:intergenic 35.0%
! AAGATGGTAACTTTTGCCTT+TGG - chr3.2:76369120-76369139 MS.gene81961:intron 35.0%
! AATTTTCCTAGCGGCATTTT+TGG + chr3.2:76369019-76369038 None:intergenic 35.0%
! ATAGAGTTTGGTTCCACAAA+GGG - chr3.2:76370078-76370097 MS.gene81961:intron 35.0%
! ATCTAATTTTGTACCTGTGG+AGG + chr3.2:76368075-76368094 None:intergenic 35.0%
! ATTGGAAGCATATTAGCGTA+AGG + chr3.2:76369827-76369846 None:intergenic 35.0%
! GACTGAATTTAACTTTGGGT+TGG + chr3.2:76367067-76367086 None:intergenic 35.0%
! GTAATGGAACAACTGAATCT+TGG + chr3.2:76369995-76370014 None:intergenic 35.0%
! TATAGAGTTTGGTTCCACAA+AGG - chr3.2:76370077-76370096 MS.gene81961:intron 35.0%
!! AAGCAAGATTTTGGTGAAGA+CGG + chr3.2:76367747-76367766 None:intergenic 35.0%
!! AGCTTGTTGGTAACAACAAT+TGG - chr3.2:76366996-76367015 MS.gene81961:CDS 35.0%
!! ATGAAATTGGTCTTGCCATT+AGG - chr3.2:76368926-76368945 MS.gene81961:intron 35.0%
!! CTTGTTGGTAACAACAATTG+GGG - chr3.2:76366998-76367017 MS.gene81961:CDS 35.0%
!! GCTTGTTGGTAACAACAATT+GGG - chr3.2:76366997-76367016 MS.gene81961:CDS 35.0%
!! TAAAACCACCTGATTTGGTT+AGG + chr3.2:76367373-76367392 None:intergenic 35.0%
!!! TCAGGTGGTTTTATTGTCTT+TGG - chr3.2:76367380-76367399 MS.gene81961:intron 35.0%
ACTAATCTGCCATATCCATG+GGG - chr3.2:76369315-76369334 MS.gene81961:CDS 40.0%
AGTCGATCACAAGTGATCAT+AGG + chr3.2:76368552-76368571 None:intergenic 40.0%
AGTGTTCGAGAACCTAGTAA+TGG + chr3.2:76370011-76370030 None:intergenic 40.0%
AGTTTCCAATCTCCTCTTTG+TGG - chr3.2:76367986-76368005 MS.gene81961:intron 40.0%
ATACACGATCAGTCCAAAAG+TGG + chr3.2:76368746-76368765 None:intergenic 40.0%
ATATGGCAGATTAGTACGGT+TGG + chr3.2:76369310-76369329 None:intergenic 40.0%
ATTACCTCCATTGAGCCATA+GGG + chr3.2:76367186-76367205 None:intergenic 40.0%
CAATCTCTTTCCATGCACAA+AGG + chr3.2:76370336-76370355 None:intergenic 40.0%
CCATCACCAGAAGGATAAAA+TGG + chr3.2:76367657-76367676 None:intergenic 40.0%
GTAACAACAATTGGGGTAGA+AGG - chr3.2:76367005-76367024 MS.gene81961:CDS 40.0%
GTACTAATCTGCCATATCCA+TGG - chr3.2:76369313-76369332 MS.gene81961:CDS 40.0%
TATTACCTCCATTGAGCCAT+AGG + chr3.2:76367187-76367206 None:intergenic 40.0%
! CACGATCCATTTTCAGAAGT+GGG + chr3.2:76368858-76368877 None:intergenic 40.0%
! CAGGAACAAAAGCATGTCAT+TGG + chr3.2:76368229-76368248 None:intergenic 40.0%
! CCATTTTATCCTTCTGGTGA+TGG - chr3.2:76367654-76367673 MS.gene81961:intron 40.0%
! TCACGATCCATTTTCAGAAG+TGG + chr3.2:76368859-76368878 None:intergenic 40.0%
! TGCTTTTGTTCCTGCTGAAA+TGG - chr3.2:76368235-76368254 MS.gene81961:CDS 40.0%
! TGGAAACCCACTTCTGAAAA+TGG - chr3.2:76368849-76368868 MS.gene81961:intron 40.0%
! TTGATTTTCGCCACTGTAAG+AGG - chr3.2:76370254-76370273 MS.gene81961:CDS 40.0%
! TTGGGTCCATTTTATCCTTC+TGG - chr3.2:76367648-76367667 MS.gene81961:intron 40.0%
!! AATTTAACTTTGGGTTGGCC+TGG + chr3.2:76367062-76367081 None:intergenic 40.0%
!! AGAATGGCATTGGCTAATTG+AGG + chr3.2:76368614-76368633 None:intergenic 40.0%
!! ATGGGAAAGTGTTGGTTTGT+TGG - chr3.2:76366948-76366967 MS.gene81961:CDS 40.0%
!! GTTTGTTGGAGTGTTGTTTG+TGG - chr3.2:76366962-76366981 MS.gene81961:CDS 40.0%
!! TCGAGGAAGTTGTTTCTTAC+CGG - chr3.2:76368282-76368301 MS.gene81961:CDS 40.0%
!!! AGGAGGTGCTTTTACTGAAT+TGG - chr3.2:76367629-76367648 MS.gene81961:intron 40.0%
!!! GGAGGTGCTTTTACTGAATT+GGG - chr3.2:76367630-76367649 MS.gene81961:intron 40.0%
ACAAAAAGCCTCTCACCCTA+TGG - chr3.2:76367168-76367187 MS.gene81961:intron 45.0%
ACTTCTCGTACCATTTCAGC+AGG + chr3.2:76368248-76368267 None:intergenic 45.0%
AGACTCCACAAAGAGGAGAT+TGG + chr3.2:76367994-76368013 None:intergenic 45.0%
CACGTATTTAGTCACCACGT+CGG + chr3.2:76367894-76367913 None:intergenic 45.0%
CTACTCCTAACCAAATCAGG+TGG - chr3.2:76367365-76367384 MS.gene81961:intron 45.0%
CTATTCAGCTCCTTTGTGCA+TGG - chr3.2:76370323-76370342 MS.gene81961:CDS 45.0%
CTGGTCACACGGAATGATTT+CGG - chr3.2:76368903-76368922 MS.gene81961:intron 45.0%
GATCTAGTTGTGAAGTTGCC+AGG - chr3.2:76367041-76367060 MS.gene81961:CDS 45.0%
GCCATATGGACAACCTTCTA+GGG - chr3.2:76370292-76370311 MS.gene81961:CDS 45.0%
GTCCTACTCCTAACCAAATC+AGG - chr3.2:76367362-76367381 MS.gene81961:intron 45.0%
TAATTACCTCAACCTGCCTG+AGG - chr3.2:76369266-76369285 MS.gene81961:CDS 45.0%
TGCCATATGGACAACCTTCT+AGG - chr3.2:76370291-76370310 MS.gene81961:CDS 45.0%
TTAATGCATGTGTAGGCTGG+AGG - chr3.2:76370206-76370225 MS.gene81961:CDS 45.0%
! CTCGCTCACAATTTTCCTAG+CGG + chr3.2:76369028-76369047 None:intergenic 45.0%
!! CACCTGATTTGGTTAGGAGT+AGG + chr3.2:76367367-76367386 None:intergenic 45.0%
!! GTGTTGTTTGTGGTGGCATA+TGG - chr3.2:76366972-76366991 MS.gene81961:CDS 45.0%
!! TGTTGGAGTGTTGTTTGTGG+TGG - chr3.2:76366965-76366984 MS.gene81961:CDS 45.0%
!! AATTATAAAAAAAATATCAT+TGG + chr3.2:76369160-76369179 None:intergenic 5.0%
!!! ATAATTTTTTAATTTGTTTT+TGG - chr3.2:76368200-76368219 MS.gene81961:intron 5.0%
!!! TAATAATAATAATTTTATGA+TGG - chr3.2:76367202-76367221 MS.gene81961:intron 5.0%
ACGAAGCCAAAAATGCCGCT+AGG - chr3.2:76369010-76369029 MS.gene81961:intron 50.0%
CGATCTAATGATCACCGACG+TGG - chr3.2:76367877-76367896 MS.gene81961:intron 50.0%
CTACACATGCCCCATGGATA+TGG + chr3.2:76369327-76369346 None:intergenic 50.0%
CTTACCGGAGAAAGCTATGC+AGG - chr3.2:76368297-76368316 MS.gene81961:CDS 50.0%
GCAGATTAGTACGGTTGGCA+TGG + chr3.2:76369305-76369324 None:intergenic 50.0%
GCCCTAGAAGGTTGTCCATA+TGG + chr3.2:76370296-76370315 None:intergenic 50.0%
GGAGGTTGGGTAACTGAGTA+TGG - chr3.2:76370224-76370243 MS.gene81961:CDS 50.0%
GGTGGCATATGGAAGCTTGT+TGG - chr3.2:76366983-76367002 MS.gene81961:CDS 50.0%
GTATGACCAACCAACTCCAG+CGG + chr3.2:76368018-76368037 None:intergenic 50.0%
TACTCACTACACATGCCCCA+TGG + chr3.2:76369333-76369352 None:intergenic 50.0%
TATGACCAACCAACTCCAGC+GGG + chr3.2:76368017-76368036 None:intergenic 50.0%
TGGACCTGCATAGCTTTCTC+CGG + chr3.2:76368304-76368323 None:intergenic 50.0%
TTTCGCCACTGTAAGAGGAG+CGG - chr3.2:76370259-76370278 MS.gene81961:CDS 50.0%
! AGGGTGAGAGGCTTTTTGTG+AGG + chr3.2:76367167-76367186 None:intergenic 50.0%
! AGTTTGGTTCCACAAAGGGC+AGG - chr3.2:76370082-76370101 MS.gene81961:intron 50.0%
! GGGTGAGAGGCTTTTTGTGA+GGG + chr3.2:76367166-76367185 None:intergenic 50.0%
! TGAGAGGCTTTTTGTGAGGG+AGG + chr3.2:76367163-76367182 None:intergenic 50.0%
!! AAAAGCACCTCCTCCGATAG+AGG + chr3.2:76367622-76367641 None:intergenic 50.0%
!! GTCTACAAGGTGCTGATACC+CGG + chr3.2:76369399-76369418 None:intergenic 50.0%
!! TATCCTTCTGGTGATGGACG+CGG - chr3.2:76367660-76367679 MS.gene81961:intron 50.0%
CAACCTGCCTGAGGTTCAGA+AGG - chr3.2:76369275-76369294 MS.gene81961:CDS 55.0%
CCATTGAGCCATAGGGTGAG+AGG + chr3.2:76367179-76367198 None:intergenic 55.0%
CCTCCGATAGAGGAACATCC+CGG + chr3.2:76367612-76367631 None:intergenic 55.0%
CCTCTCACCCTATGGCTCAA+TGG - chr3.2:76367176-76367195 MS.gene81961:intron 55.0%
CTCACCCTATGGCTCAATGG+AGG - chr3.2:76367179-76367198 MS.gene81961:intron 55.0%
GCATGTGTAGGCTGGAGGTT+GGG - chr3.2:76370211-76370230 MS.gene81961:CDS 55.0%
GGATGTTCCTCTATCGGAGG+AGG - chr3.2:76367612-76367631 MS.gene81961:intron 55.0%
GGTCCGGGATGTTCCTCTAT+CGG - chr3.2:76367606-76367625 MS.gene81961:intron 55.0%
TCTGAACCTCAGGCAGGTTG+AGG + chr3.2:76369275-76369294 None:intergenic 55.0%
TGCATGTGTAGGCTGGAGGT+TGG - chr3.2:76370210-76370229 MS.gene81961:CDS 55.0%
TGGAGAGCCTTCTGAACCTC+AGG + chr3.2:76369285-76369304 None:intergenic 55.0%
TGTAAGAGGAGCGGGTCACA+TGG - chr3.2:76370268-76370287 MS.gene81961:CDS 55.0%
TTCGCCACTGTAAGAGGAGC+GGG - chr3.2:76370260-76370279 MS.gene81961:CDS 55.0%
! GAGGCTTTTTGTGAGGGAGG+TGG + chr3.2:76367160-76367179 None:intergenic 55.0%
!! AACATTGGTGACGCCTCCAC+AGG - chr3.2:76368059-76368078 MS.gene81961:CDS 55.0%
AGACCGCGTCCATCACCAGA+AGG + chr3.2:76367666-76367685 None:intergenic 60.0%
CAGCGGGAGACTCCACAAAG+AGG + chr3.2:76368001-76368020 None:intergenic 60.0%
CCGGGATGTTCCTCTATCGG+AGG - chr3.2:76367609-76367628 MS.gene81961:intron 60.0%
CTCTTTGTGGAGTCTCCCGC+TGG - chr3.2:76367999-76368018 MS.gene81961:CDS 60.0%
GAGCCTTCTGAACCTCAGGC+AGG + chr3.2:76369281-76369300 None:intergenic 60.0%
GTGACCCGCTCCTCTTACAG+TGG + chr3.2:76370267-76370286 None:intergenic 60.0%
! AGTCTCCCGCTGGAGTTGGT+TGG - chr3.2:76368009-76368028 MS.gene81961:CDS 60.0%
!! GCGGGTCACATGGTGCCATA+TGG - chr3.2:76370278-76370297 MS.gene81961:CDS 60.0%
GTGGAGTCTCCCGCTGGAGT+TGG - chr3.2:76368005-76368024 MS.gene81961:CDS 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 76366948 76370388 76366948 ID=MS.gene81961
chr3.2 mRNA 76366948 76370388 76366948 ID=MS.gene81961.t1;Parent=MS.gene81961
chr3.2 exon 76370136 76370388 76370136 ID=MS.gene81961.t1.exon1;Parent=MS.gene81961.t1
chr3.2 CDS 76370136 76370388 76370136 ID=cds.MS.gene81961.t1;Parent=MS.gene81961.t1
chr3.2 exon 76369622 76369729 76369622 ID=MS.gene81961.t1.exon2;Parent=MS.gene81961.t1
chr3.2 CDS 76369622 76369729 76369622 ID=cds.MS.gene81961.t1;Parent=MS.gene81961.t1
chr3.2 exon 76369256 76369348 76369256 ID=MS.gene81961.t1.exon3;Parent=MS.gene81961.t1
chr3.2 CDS 76369256 76369348 76369256 ID=cds.MS.gene81961.t1;Parent=MS.gene81961.t1
chr3.2 exon 76369018 76369110 76369018 ID=MS.gene81961.t1.exon4;Parent=MS.gene81961.t1
chr3.2 CDS 76369018 76369110 76369018 ID=cds.MS.gene81961.t1;Parent=MS.gene81961.t1
chr3.2 exon 76368651 76368736 76368651 ID=MS.gene81961.t1.exon5;Parent=MS.gene81961.t1
chr3.2 CDS 76368651 76368736 76368651 ID=cds.MS.gene81961.t1;Parent=MS.gene81961.t1
chr3.2 exon 76368211 76368489 76368211 ID=MS.gene81961.t1.exon6;Parent=MS.gene81961.t1
chr3.2 CDS 76368211 76368489 76368211 ID=cds.MS.gene81961.t1;Parent=MS.gene81961.t1
chr3.2 exon 76367987 76368120 76367987 ID=MS.gene81961.t1.exon7;Parent=MS.gene81961.t1
chr3.2 CDS 76367987 76368120 76367987 ID=cds.MS.gene81961.t1;Parent=MS.gene81961.t1
chr3.2 exon 76367445 76367537 76367445 ID=MS.gene81961.t1.exon8;Parent=MS.gene81961.t1
chr3.2 CDS 76367445 76367537 76367445 ID=cds.MS.gene81961.t1;Parent=MS.gene81961.t1
chr3.2 exon 76367233 76367350 76367233 ID=MS.gene81961.t1.exon9;Parent=MS.gene81961.t1
chr3.2 CDS 76367233 76367350 76367233 ID=cds.MS.gene81961.t1;Parent=MS.gene81961.t1
chr3.2 exon 76366948 76367115 76366948 ID=MS.gene81961.t1.exon10;Parent=MS.gene81961.t1
chr3.2 CDS 76366948 76367115 76366948 ID=cds.MS.gene81961.t1;Parent=MS.gene81961.t1
Gene Sequence

>MS.gene81961

ATGGGAAAGTGTTGGTTTGTTGGAGTGTTGTTTGTGGTGGCATATGGAAGCTTGTTGGTAACAACAATTGGGGTAGAAGGGTACCCAATTGAAGATCTAGTTGTGAAGTTGCCAGGCCAACCCAAAGTTAAATTCAGTCAATATGCTGGTTATGTTGATATAGATATAAAACATGGGAGGAGTCTCTTCTATTATTTTGTTGAGGCTGACCACCTCCCTCACAAAAAGCCTCTCACCCTATGGCTCAATGGAGGTAATAATAATAATTTTATGATGGATGCATATCATTTTTACTCTTCATTACCTTGTTGTCACAAGTTTTTCCTTTATTTAATTTCGTATGTTGATAGTATAGGTTGATGTCATTTATTTGTTCTTGTATTATTGTACTGTGTTATGTGCTTGTGTTGTATTGTCCTACTCCTAACCAAATCAGGTGGTTTTATTGTCTTTGGTAGTAGAAAATCTTACCAGCTTAACAAAATTATATCATGTATATCAAAATTGTTGAATTATTTTAGCTCCTTTTTCTTTATTTTAAGATGTAGATATTTGTTAATTTTTTATAACGCCTACATCAAATTCAAATCATCTTACAAGGATCATAAATAAATTGTTCTTTATTGGAGGGGAATTAATTAACTTGATTAATTTTCTAGGTCCGGGATGTTCCTCTATCGGAGGAGGTGCTTTTACTGAATTGGGTCCATTTTATCCTTCTGGTGATGGACGCGGTCTACGAAGAAATTCAAAGTCCTGGAATACAGGTATGTATATGACTATGAATTTGAATTCACCGTCTTCACCAAAATCTTGCTTTCATGTAGTCATTATATCTGTGAGTTTTAAGATCCAGATTAGACAAAGCAAATTTCAAGATCAGGCTTTTAGTTTTAAAAAATCTTTTTGATATGAGCACAATACGATATCGATCTAATGATCACCGACGTGGTGACTAAATACGTGTTTTGATATTTGAGAATTATGTGGGTGTATTAATTCCTGACTTATATATTGTTCATGTCTACTCTTTTTTATAGTTTCCAATCTCCTCTTTGTGGAGTCTCCCGCTGGAGTTGGTTGGTCATACTCAAATACAACTTCTGATTATAACATTGGTGACGCCTCCACAGGTACAAAATTAGATTTGTGAGACCAAATTTATGTAGTCAATATCTATACAAAAGAATAAAAAAAAATATTTTTTGTATGTTATACATGGAATTTGTTAGTTTTATTATTAGGCATGAACATAATTTTTTAATTTGTTTTTGGCAGCCAATGACATGCTTTTGTTCCTGCTGAAATGGTACGAGAAGTTTCCTTCATACAAATCGAGGAAGTTGTTTCTTACCGGAGAAAGCTATGCAGGTCCAACAAACACAATAATCAAATTCCAAATTTAATAATTTATCGTTTCAAAATTCCAAAAATTAAATTTAGTGTCGTGGTATAAAATATTCAATTTAGCAGAGAGGATTCAGTTATATAAATTTTATATTCTTTATGCATGCACACAAAACCTATTTCATCATTTCTGGCAGAGACATACATACACAAATGTTTAACACAAACATTTGTTCCTTAATTGTGTTCTTTTGCCTATGATCACTTGTGATCGACTTTTGTTTCTTACATACTAACATTTTCAGGACATTACATACCTCAATTAGCCAATGCCATTCTTGATTATAATGCTCATTCATCCAGTTTCAAGTTCAACATAAAAGGAGTTGCCGTAAGATCCAATTCACATGATATATTAAATTAATTTACTCACAACTATATATTTGTGCCACTTTTGGACTGATCGTGTATATTTGATGACTTATTGATTATTTAATTAAGAAATTTTGAAAGTTAATTAAACTGATCTTACTTTTCCCTTAACTTTGGTAGATTGGAAACCCACTTCTGAAAATGGATCGTGATACACAAGCAACATATGAATATTACTGGTCACACGGAATGATTTCGGATGAAATTGGTCTTGCCATTAGGAATGATTGTGATTTTGATGATCATACCCATGCAACTACTCATAACGTATCTAAATCATGCAACGAAGCCAAAAATGCCGCTAGGAAAATTGTGAGCGAGTATGTAGACAACTATGATGTGATTCTTGATGTGTGTTATCCAGCTATAGCTGAACAAGAAATCAGATTGAAAAAGATGGTAACTTTTGCCTTTGGATTATTATGTTCTGCCAATGATATTTTTTTTATAATTAATTAATTAATTAATTTGTTTATTTCATTAATGCAGGCTACTAAAATAAGTTTAAGTGTAGACGTTTGTATAGATTATGAAAGCTTTAATTACCTCAACCTGCCTGAGGTTCAGAAGGCTCTCCATGCCAACCGTACTAATCTGCCATATCCATGGGGCATGTGTAGTGAGTAAGTATAAATAAAGCAAAAATATATTTGTTATCATGAAGTTTTACCGGGTATCAGCACCTTGTAGACAAAATTAATTTAACATTTAATTTGATTTCAAAATAATTATGTATAAAAAATGACATAACTATAGATTGTATTATAAATAAATTAGAATACACTTTGATATATAAATATTATTGGAAAAAGACAATTTCATAACTAGAGATATAATTGATATGAATGGAGTTTATAAATATAGGCAACATTCATTTTATTATATGTTGATTTTGTGAATTAAGTTAAGACTAACCATCAAATTTACTAGCTAGGAAACTAATATTGCTAATTTTTTTTTTTAATAAATATATTTGAAATCTTGTCTCATATGATCATAGTAAACATGTCATTTTTGTTTTAATTTTGATTACCGAAGAAATAACAGCTCATGTACATTTTCTTCATTACAGTGTTTTGAATTACAGCAAGACAGATCCTTACGCTAATATGCTTCCAATTCTCAAACGGATAGTTCAAAATCATATTCCAATATGGGTTTATAGGTAGTTAATTTTAACAAATGATAATCATTTGCTATTATTTATTTTATATATTTTTGGAATAAGCCTTACCATACTTGTTAAATTATTATGATAGTGGAGACCAAGATTCAGTTGTTCCATTACTAGGTTCTCGAACACTCATTCGTGAACTAGCTCATGACTTGAAATTCAAGATTACAGACTCATATAGAGTTTGGTTCCACAAAGGGCAGGTAAATATATATTTTTATTAACAATTAACCTAATTTTTTTGCATATTGAAAATTGATTTGAGTATGGATTTCGCAATTTTATTAACTCATGTTTCTAAAAAATTAATGCATGTGTAGGCTGGAGGTTGGGTAACTGAGTATGGAAATTTGTTGATTTTCGCCACTGTAAGAGGAGCGGGTCACATGGTGCCATATGGACAACCTTCTAGGGCATTACATCTATTCAGCTCCTTTGTGCATGGAAAGAGATTGCCAAACACTACTAAACCCTCAATTGGAGAATGA
Protein sequence

>MS.gene81961.t1

MGKCWFVGVLFVVAYGSLLVTTIGVEGYPIEDLVVKLPGQPKVKFSQYAGYVDIDIKHGRSLFYYFVEADHLPHKKPLTLWLNGGPGCSSIGGGAFTELGPFYPSGDGRGLRRNSKSWNTVSNLLFVESPAGVGWSYSNTTSDYNIGDASTANDMLLFLLKWYEKFPSYKSRKLFLTGESYAGHYIPQLANAILDYNAHSSSFKFNIKGVAIGNPLLKMDRDTQATYEYYWSHGMISDEIGLAIRNDCDFDDHTHATTHNVSKSCNEAKNAARKIVSEYVDNYDVILDVCYPAIAEQEIRLKKMATKISLSVDVCIDYESFNYLNLPEVQKALHANRTNLPYPWGMCSDVLNYSKTDPYANMLPILKRIVQNHIPIWVYSGDQDSVVPLLGSRTLIRELAHDLKFKITDSYRVWFHKGQAGGWVTEYGNLLIFATVRGAGHMVPYGQPSRALHLFSSFVHGKRLPNTTKPSIGE