Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82210.t1 | RHN59397.1 | 95 | 119 | 6 | 0 | 1 | 119 | 22 | 140 | 2.10E-54 | 221.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82210.t1 | G7JIA9 | 95.0 | 119 | 6 | 0 | 1 | 119 | 1 | 119 | 1.5e-54 | 221.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051720 | MS.gene82210 | 0.800704 | 1.27E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82210.t1 | MTR_4g028110 | 94.958 | 119 | 6 | 0 | 1 | 119 | 1 | 119 | 1.98e-78 | 226 |
| MS.gene82210.t1 | MTR_7g073270 | 49.593 | 123 | 52 | 5 | 4 | 119 | 1 | 120 | 1.09e-23 | 88.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATCAGAACCAAGATTTCTA+AGG | 0.315908 | 4.2:-75626204 | MS.gene82210:CDS |
| GGAAGACGTTGCCGCGGTTC+CGG | 0.317179 | 4.2:-75626112 | MS.gene82210:CDS |
| GACACTGACCTTAGAAATCT+TGG | 0.321072 | 4.2:+75626196 | None:intergenic |
| GCAATGATTCGAAAGAGATT+TGG | 0.327865 | 4.2:-75626310 | MS.gene82210:CDS |
| TCAGAATCCACTCTCTTTCA+AGG | 0.337545 | 4.2:-75626011 | MS.gene82210:CDS |
| CTTAAACACCTTTCGAAAAC+CGG | 0.347709 | 4.2:+75626093 | None:intergenic |
| AAATTGTTCAAACCATTTCT+TGG | 0.349128 | 4.2:-75626070 | MS.gene82210:CDS |
| CAAAGGATAGCCTCTCAAAT+TGG | 0.423678 | 4.2:+75626263 | None:intergenic |
| TGAGTGACAAGAGGAACTGC+TGG | 0.485543 | 4.2:+75626148 | None:intergenic |
| AGCAGTTCCTCTTGTCACTC+AGG | 0.488848 | 4.2:-75626146 | MS.gene82210:CDS |
| TTTGTTGAGTTGAGACTCAA+AGG | 0.523465 | 4.2:+75626246 | None:intergenic |
| TGATAAGCTTCTTGATAACA+TGG | 0.525013 | 4.2:+75626222 | None:intergenic |
| CTAAATGCCTTGAAAGAGAG+TGG | 0.545008 | 4.2:+75626004 | None:intergenic |
| GTCACTCAGGAGGCGAAGCA+AGG | 0.560619 | 4.2:-75626133 | MS.gene82210:CDS |
| AGGCATTTAGTAGATCATTG+AGG | 0.589944 | 4.2:-75625991 | MS.gene82210:CDS |
| TTCGCCTCCTGAGTGACAAG+AGG | 0.607306 | 4.2:+75626139 | None:intergenic |
| ATTTGTTAGACCAATTTGAG+AGG | 0.623039 | 4.2:-75626273 | MS.gene82210:CDS |
| CCTTTCGAAAACCGGAACCG+CGG | 0.654266 | 4.2:+75626101 | None:intergenic |
| AGTTCCTCTTGTCACTCAGG+AGG | 0.660481 | 4.2:-75626143 | MS.gene82210:CDS |
| AAGCAAGGAAGACGTTGCCG+CGG | 0.700615 | 4.2:-75626118 | MS.gene82210:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAATTGTTCAAACCATTTCT+TGG | - | chr4.2:75626237-75626256 | MS.gene82210:CDS | 25.0% |
| CCATTTCTTGGTAGAAAAAA+TGG | - | chr4.2:75626249-75626268 | MS.gene82210:CDS | 30.0% | |
| TATCAGAACCAAGATTTCTA+AGG | - | chr4.2:75626103-75626122 | MS.gene82210:CDS | 30.0% | |
| ! | ATTTGTTAGACCAATTTGAG+AGG | - | chr4.2:75626034-75626053 | MS.gene82210:CDS | 30.0% |
| ! | TGATAAGCTTCTTGATAACA+TGG | + | chr4.2:75626088-75626107 | None:intergenic | 30.0% |
| !!! | CCATTTTTTCTACCAAGAAA+TGG | + | chr4.2:75626252-75626271 | None:intergenic | 30.0% |
| AGGCATTTAGTAGATCATTG+AGG | - | chr4.2:75626316-75626335 | MS.gene82210:CDS | 35.0% | |
| CTTAAACACCTTTCGAAAAC+CGG | + | chr4.2:75626217-75626236 | None:intergenic | 35.0% | |
| GCAATGATTCGAAAGAGATT+TGG | - | chr4.2:75625997-75626016 | MS.gene82210:CDS | 35.0% | |
| TTGGTAGAAAAAATGGAGCA+AGG | - | chr4.2:75626256-75626275 | MS.gene82210:CDS | 35.0% | |
| !! | TTTGTTGAGTTGAGACTCAA+AGG | + | chr4.2:75626064-75626083 | None:intergenic | 35.0% |
| CAAAGGATAGCCTCTCAAAT+TGG | + | chr4.2:75626047-75626066 | None:intergenic | 40.0% | |
| CTAAATGCCTTGAAAGAGAG+TGG | + | chr4.2:75626306-75626325 | None:intergenic | 40.0% | |
| GACACTGACCTTAGAAATCT+TGG | + | chr4.2:75626114-75626133 | None:intergenic | 40.0% | |
| TCAGAATCCACTCTCTTTCA+AGG | - | chr4.2:75626296-75626315 | MS.gene82210:CDS | 40.0% | |
| AGCAGTTCCTCTTGTCACTC+AGG | - | chr4.2:75626161-75626180 | MS.gene82210:CDS | 50.0% | |
| AGTTCCTCTTGTCACTCAGG+AGG | - | chr4.2:75626164-75626183 | MS.gene82210:CDS | 50.0% | |
| TGAGTGACAAGAGGAACTGC+TGG | + | chr4.2:75626162-75626181 | None:intergenic | 50.0% | |
| AAGCAAGGAAGACGTTGCCG+CGG | - | chr4.2:75626189-75626208 | MS.gene82210:CDS | 55.0% | |
| CCTTTCGAAAACCGGAACCG+CGG | + | chr4.2:75626209-75626228 | None:intergenic | 55.0% | |
| TTCGCCTCCTGAGTGACAAG+AGG | + | chr4.2:75626171-75626190 | None:intergenic | 55.0% | |
| GTCACTCAGGAGGCGAAGCA+AGG | - | chr4.2:75626174-75626193 | MS.gene82210:CDS | 60.0% | |
| ! | CCGCGGTTCCGGTTTTCGAA+AGG | - | chr4.2:75626206-75626225 | MS.gene82210:CDS | 60.0% |
| GGAAGACGTTGCCGCGGTTC+CGG | - | chr4.2:75626195-75626214 | MS.gene82210:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 75625985 | 75626344 | 75625985 | ID=MS.gene82210 |
| chr4.2 | mRNA | 75625985 | 75626344 | 75625985 | ID=MS.gene82210.t1;Parent=MS.gene82210 |
| chr4.2 | exon | 75625985 | 75626344 | 75625985 | ID=MS.gene82210.t1.exon1;Parent=MS.gene82210.t1 |
| chr4.2 | CDS | 75625985 | 75626344 | 75625985 | ID=cds.MS.gene82210.t1;Parent=MS.gene82210.t1 |
| Gene Sequence |
| Protein sequence |
