Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82381.t1 | AES90005.1 | 98.9 | 89 | 1 | 0 | 1 | 89 | 1 | 89 | 6.10E-06 | 60.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82381.t1 | G7JHI8 | 98.9 | 89 | 1 | 0 | 1 | 89 | 1 | 89 | 4.4e-06 | 60.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052376 | MS.gene82381 | 0.814738 | 1.32E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82381.t1 | MTR_4g083130 | 96.629 | 89 | 3 | 0 | 1 | 89 | 1 | 89 | 1.19e-57 | 172 |
| MS.gene82381.t1 | MTR_4g083110 | 80.899 | 89 | 13 | 1 | 1 | 89 | 1 | 85 | 6.38e-40 | 127 |
| MS.gene82381.t1 | MTR_5g077640 | 77.528 | 89 | 20 | 0 | 1 | 89 | 1 | 89 | 3.68e-36 | 117 |
| MS.gene82381.t1 | MTR_5g077680 | 78.846 | 52 | 11 | 0 | 1 | 52 | 1 | 52 | 1.30e-23 | 85.5 |
| MS.gene82381.t1 | MTR_8g079458 | 78.846 | 52 | 11 | 0 | 1 | 52 | 1 | 52 | 1.30e-23 | 85.5 |
| MS.gene82381.t1 | MTR_4g012020 | 76.923 | 52 | 12 | 0 | 1 | 52 | 1 | 52 | 5.15e-23 | 84.0 |
| MS.gene82381.t1 | MTR_1437s0010 | 75.000 | 52 | 13 | 0 | 1 | 52 | 1 | 52 | 2.05e-22 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82381.t1 | AT1G30260 | 60.417 | 48 | 18 | 1 | 1 | 48 | 1 | 47 | 3.59e-15 | 65.1 |
Find 12 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAATGCAGTTGTTAATTCT+AGG | 0.257861 | 4.2:+26398772 | MS.gene82381:CDS |
| AACAATCACTGAAGAGGATA+TGG | 0.331678 | 4.2:+26398665 | MS.gene82381:CDS |
| GTGTTCTATGAACTTCCTTC+AGG | 0.381163 | 4.2:-26398800 | None:intergenic |
| GCGTAGAACATCAAAGATGA+TGG | 0.510830 | 4.2:+26398641 | MS.gene82381:CDS |
| CCTAGAATTAACAACTGCAT+TGG | 0.551602 | 4.2:-26398772 | None:intergenic |
| AACAACTGCATTGGTAGAAG+AGG | 0.557920 | 4.2:-26398763 | None:intergenic |
| TGGCGAATTCTCGCATTGCA+AGG | 0.566271 | 4.2:+26398574 | MS.gene82381:CDS |
| CACAGTATGTTAGCGTGATG+AGG | 0.569645 | 4.2:+26398619 | MS.gene82381:CDS |
| TAATTCTAGGTACTTCCTGA+AGG | 0.589595 | 4.2:+26398785 | MS.gene82381:CDS |
| GATGGAAACAATCACTGAAG+AGG | 0.591147 | 4.2:+26398659 | MS.gene82381:CDS |
| CTCATCACGCTAACATACTG+TGG | 0.654266 | 4.2:-26398618 | None:intergenic |
| ATCACGCTAACATACTGTGG+TGG | 0.690677 | 4.2:-26398615 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GATGAAGTTGATGAACTTTT+AGG | - | chr4.2:26398723-26398742 | None:intergenic | 30.0% |
| AACAATCACTGAAGAGGATA+TGG | + | chr4.2:26398665-26398684 | MS.gene82381:CDS | 35.0% | |
| CCAATGCAGTTGTTAATTCT+AGG | + | chr4.2:26398772-26398791 | MS.gene82381:CDS | 35.0% | |
| CCTAGAATTAACAACTGCAT+TGG | - | chr4.2:26398775-26398794 | None:intergenic | 35.0% | |
| TAATTCTAGGTACTTCCTGA+AGG | + | chr4.2:26398785-26398804 | MS.gene82381:CDS | 35.0% | |
| !!! | GAAGTTGATGAACTTTTAGG+TGG | - | chr4.2:26398720-26398739 | None:intergenic | 35.0% |
| GATGGAAACAATCACTGAAG+AGG | + | chr4.2:26398659-26398678 | MS.gene82381:CDS | 40.0% | |
| GCGTAGAACATCAAAGATGA+TGG | + | chr4.2:26398641-26398660 | MS.gene82381:CDS | 40.0% | |
| GTGTTCTATGAACTTCCTTC+AGG | - | chr4.2:26398803-26398822 | None:intergenic | 40.0% | |
| ! | AACAACTGCATTGGTAGAAG+AGG | - | chr4.2:26398766-26398785 | None:intergenic | 40.0% |
| ! | CATTGCAAGGTTTTTCATGG+AGG | + | chr4.2:26398587-26398606 | MS.gene82381:CDS | 40.0% |
| ! | TCGCATTGCAAGGTTTTTCA+TGG | + | chr4.2:26398584-26398603 | MS.gene82381:CDS | 40.0% |
| !! | GTTGATGAACTTTTAGGTGG+TGG | - | chr4.2:26398717-26398736 | None:intergenic | 40.0% |
| ATCACGCTAACATACTGTGG+TGG | - | chr4.2:26398618-26398637 | None:intergenic | 45.0% | |
| CACAGTATGTTAGCGTGATG+AGG | + | chr4.2:26398619-26398638 | MS.gene82381:CDS | 45.0% | |
| CTCATCACGCTAACATACTG+TGG | - | chr4.2:26398621-26398640 | None:intergenic | 45.0% | |
| ! | TGCAAGGTTTTTCATGGAGG+TGG | + | chr4.2:26398590-26398609 | MS.gene82381:CDS | 45.0% |
| TGGCGAATTCTCGCATTGCA+AGG | + | chr4.2:26398574-26398593 | MS.gene82381:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26398573 | 26398842 | 26398573 | ID=MS.gene82381 |
| chr4.2 | mRNA | 26398573 | 26398842 | 26398573 | ID=MS.gene82381.t1;Parent=MS.gene82381 |
| chr4.2 | exon | 26398573 | 26398842 | 26398573 | ID=MS.gene82381.t1.exon1;Parent=MS.gene82381.t1 |
| chr4.2 | CDS | 26398573 | 26398842 | 26398573 | ID=cds.MS.gene82381.t1;Parent=MS.gene82381.t1 |
| Gene Sequence |
| Protein sequence |
