Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82385.t1 | NP_001235961.1 | 100 | 58 | 0 | 0 | 1 | 58 | 40 | 97 | 4.70E-23 | 116.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82385.t1 | Q9LMN4 | 93.0 | 57 | 4 | 0 | 1 | 57 | 40 | 96 | 2.1e-24 | 112.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82385.t1 | A0A0B2SAJ4 | 100.0 | 58 | 0 | 0 | 1 | 58 | 40 | 97 | 3.4e-23 | 116.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051903 | MS.gene82385 | 0.800041 | 1.74E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82385.t1 | MTR_4g083170 | 100.000 | 58 | 0 | 0 | 1 | 58 | 41 | 98 | 1.38e-36 | 117 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82385.t1 | AT1G21190 | 91.379 | 58 | 5 | 0 | 1 | 58 | 40 | 97 | 1.52e-34 | 112 |
| MS.gene82385.t1 | AT1G76860 | 91.379 | 58 | 5 | 0 | 1 | 58 | 40 | 97 | 1.22e-33 | 110 |
Find 15 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGTTCCGTTCCTGTTTGTT+AGG | 0.219777 | 4.2:+26363953 | MS.gene82385:CDS |
| CAACATCTTAATATGATTCT+TGG | 0.270259 | 4.2:+26362716 | MS.gene82385:CDS |
| CGCCATCTCCCCTAACAAAC+AGG | 0.401332 | 4.2:-26363963 | None:intergenic |
| TTCCTGTTTGTTAGGGGAGA+TGG | 0.402900 | 4.2:+26363961 | MS.gene82385:CDS |
| TAGGGGAGATGGCGTGATAT+TGG | 0.403792 | 4.2:+26363972 | MS.gene82385:CDS |
| AGTTCCGTTCCTGTTTGTTA+GGG | 0.430031 | 4.2:+26363954 | MS.gene82385:CDS |
| AGACATACGAAGAAATTGTC+AGG | 0.452612 | 4.2:+26362780 | MS.gene82385:CDS |
| GTTCCGTTCCTGTTTGTTAG+GGG | 0.578832 | 4.2:+26363955 | MS.gene82385:CDS |
| CCATGTTATGCAGTCCTCAG+TGG | 0.619717 | 4.2:-26364003 | None:intergenic |
| TGTTATGCAGTCCTCAGTGG+TGG | 0.628383 | 4.2:-26364000 | None:intergenic |
| TGTTGAAGAAATTGTTACAA+CGG | 0.651927 | 4.2:+26362742 | MS.gene82385:CDS |
| GACATACGAAGAAATTGTCA+GGG | 0.666267 | 4.2:+26362781 | MS.gene82385:CDS |
| TCTCCCCTAACAAACAGGAA+CGG | 0.688766 | 4.2:-26363958 | None:intergenic |
| TCTGATTGCAGACTACAAAG+CGG | 0.697746 | 4.2:+26363929 | MS.gene82385:intron |
| TATTGGTTTCTCCACCACTG+AGG | 0.734600 | 4.2:+26363989 | MS.gene82385:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGAAACTTTTGAATAAAATT+TGG | + | chr4.2:26363550-26363569 | MS.gene82385:intron | 15.0% |
| !! | AATTTAAATTCTCCAATTTG+AGG | + | chr4.2:26363287-26363306 | MS.gene82385:intron | 20.0% |
| !! | CAAAATGGTCTATAAAAAAA+AGG | + | chr4.2:26363849-26363868 | MS.gene82385:intron | 20.0% |
| !! | GAACTAAAAAATTGAAATAG+CGG | + | chr4.2:26363309-26363328 | MS.gene82385:intron | 20.0% |
| !!! | AATTTTTTAGTTCCTCAAAT+TGG | - | chr4.2:26363302-26363321 | None:intergenic | 20.0% |
| !!! | ATCACAATTCTGAAAAAATA+AGG | - | chr4.2:26363705-26363724 | None:intergenic | 20.0% |
| !!! | TTGAGAGTATTATAAAATGA+GGG | - | chr4.2:26363384-26363403 | None:intergenic | 20.0% |
| !!! | TTTTGAATAAAATTTGGTTG+TGG | + | chr4.2:26363556-26363575 | MS.gene82385:intron | 20.0% |
| !!! | TTTTTTATAGACCATTTTGA+TGG | - | chr4.2:26363848-26363867 | None:intergenic | 20.0% |
| ! | AGAAAAACAAAAATCATCCA+AGG | - | chr4.2:26363909-26363928 | None:intergenic | 25.0% |
| ! | CAACATCTTAATATGATTCT+TGG | + | chr4.2:26362716-26362735 | MS.gene82385:CDS | 25.0% |
| ! | TGTTGAAGAAATTGTTACAA+CGG | + | chr4.2:26362742-26362761 | MS.gene82385:CDS | 25.0% |
| ! | TTATTCAAAAGTTTCTCCTT+CGG | - | chr4.2:26363546-26363565 | None:intergenic | 25.0% |
| !! | AAGGATTATTGTAGTTTGTA+AGG | + | chr4.2:26362909-26362928 | MS.gene82385:intron | 25.0% |
| !! | TAAAAAATTGAAATAGCGGA+GGG | + | chr4.2:26363313-26363332 | MS.gene82385:intron | 25.0% |
| !!! | ATTTTGAAGATACTTTTCCT+TGG | + | chr4.2:26363889-26363908 | MS.gene82385:intron | 25.0% |
| !!! | GTTGAGAGTATTATAAAATG+AGG | - | chr4.2:26363385-26363404 | None:intergenic | 25.0% |
| !!! | TATTTTTTCGATTAGATTGC+TGG | + | chr4.2:26363659-26363678 | MS.gene82385:intron | 25.0% |
| !!! | TGAGAGTATTATAAAATGAG+GGG | - | chr4.2:26363383-26363402 | None:intergenic | 25.0% |
| ACACCTTAAATCTGAATTGT+TGG | + | chr4.2:26363071-26363090 | MS.gene82385:intron | 30.0% | |
| AGGTCAATAGTTGAAAGAAA+AGG | - | chr4.2:26363685-26363704 | None:intergenic | 30.0% | |
| ATCCTTTATCAAACTGAAGA+AGG | - | chr4.2:26362895-26362914 | None:intergenic | 30.0% | |
| CTAAAAAATTGAAATAGCGG+AGG | + | chr4.2:26363312-26363331 | MS.gene82385:intron | 30.0% | |
| GATAAGAAAAGGATCATCTT+TGG | + | chr4.2:26363763-26363782 | MS.gene82385:intron | 30.0% | |
| TACCTTCTTCAGTTTGATAA+AGG | + | chr4.2:26362890-26362909 | MS.gene82385:intron | 30.0% | |
| ! | AAGATGATCCTTTTCTTATC+TGG | - | chr4.2:26363763-26363782 | None:intergenic | 30.0% |
| ! | AGATGATCCTTTTCTTATCT+GGG | - | chr4.2:26363762-26363781 | None:intergenic | 30.0% |
| ! | ATATCTACTAACTTGTGATC+TGG | + | chr4.2:26363723-26363742 | MS.gene82385:intron | 30.0% |
| ! | ATGCTTAGGAGAATTTTTCA+AGG | - | chr4.2:26363359-26363378 | None:intergenic | 30.0% |
| ! | TATCTACTAACTTGTGATCT+GGG | + | chr4.2:26363724-26363743 | MS.gene82385:intron | 30.0% |
| !! | ACAGTTGTTGATTTATTGTC+AGG | + | chr4.2:26362845-26362864 | MS.gene82385:intron | 30.0% |
| !! | CAGTTGTTGATTTATTGTCA+GGG | + | chr4.2:26362846-26362865 | MS.gene82385:intron | 30.0% |
| !! | GTAGTTTGTAAGGTGTTTTA+AGG | + | chr4.2:26362919-26362938 | MS.gene82385:intron | 30.0% |
| !! | TTATAGACCATTTTGATGGT+TGG | - | chr4.2:26363844-26363863 | None:intergenic | 30.0% |
| AGACATACGAAGAAATTGTC+AGG | + | chr4.2:26362780-26362799 | MS.gene82385:CDS | 35.0% | |
| ATAAAATGAGGGGAATGCTT+AGG | - | chr4.2:26363373-26363392 | None:intergenic | 35.0% | |
| ATAAATCAACAACTGTTCGC+AGG | - | chr4.2:26362841-26362860 | None:intergenic | 35.0% | |
| CTGCTTTCAATAATCATCAC+CGG | - | chr4.2:26363130-26363149 | None:intergenic | 35.0% | |
| GAAAAATAAACCGCAAAGCA+AGG | - | chr4.2:26363261-26363280 | None:intergenic | 35.0% | |
| GACATACGAAGAAATTGTCA+GGG | + | chr4.2:26362781-26362800 | MS.gene82385:CDS | 35.0% | |
| GAGAATCCTTCAACTTGATA+GGG | - | chr4.2:26363458-26363477 | None:intergenic | 35.0% | |
| GCACCAACAATTCAGATTTA+AGG | - | chr4.2:26363077-26363096 | None:intergenic | 35.0% | |
| TGACACTAACACTTCAATAC+CGG | + | chr4.2:26363108-26363127 | MS.gene82385:intron | 35.0% | |
| TGTCTTTCCCAGATAAGAAA+AGG | + | chr4.2:26363752-26363771 | MS.gene82385:intron | 35.0% | |
| TGTGTGAGTTAAGAACTATG+AGG | + | chr4.2:26362949-26362968 | MS.gene82385:intron | 35.0% | |
| !! | AGAACTATGAGGCATTGATA+CGG | + | chr4.2:26362960-26362979 | MS.gene82385:intron | 35.0% |
| AATCACGATGGGAAAGGATT+TGG | - | chr4.2:26363512-26363531 | None:intergenic | 40.0% | |
| ATCACGATGGGAAAGGATTT+GGG | - | chr4.2:26363511-26363530 | None:intergenic | 40.0% | |
| ATTGAAGTGGAGGGAAAGAA+AGG | - | chr4.2:26363480-26363499 | None:intergenic | 40.0% | |
| CTTTGTTCAGGAATCACGAT+GGG | - | chr4.2:26363523-26363542 | None:intergenic | 40.0% | |
| GGAGAATCCTTCAACTTGAT+AGG | - | chr4.2:26363459-26363478 | None:intergenic | 40.0% | |
| TCTGATTGCAGACTACAAAG+CGG | + | chr4.2:26363929-26363948 | MS.gene82385:intron | 40.0% | |
| TGTCTAGTAGCTGACATATG+TGG | - | chr4.2:26363153-26363172 | None:intergenic | 40.0% | |
| ! | AGTTCCGTTCCTGTTTGTTA+GGG | + | chr4.2:26363954-26363973 | MS.gene82385:CDS | 40.0% |
| ! | GCAGGTCTACAATTTAGCTT+TGG | - | chr4.2:26362823-26362842 | None:intergenic | 40.0% |
| !! | CTGAAATGTTGGTGCTACAT+AGG | + | chr4.2:26363183-26363202 | MS.gene82385:intron | 40.0% |
| !! | GAACTATGAGGCATTGATAC+GGG | + | chr4.2:26362961-26362980 | MS.gene82385:intron | 40.0% |
| AATAAACCGCAAAGCAAGGC+TGG | - | chr4.2:26363257-26363276 | None:intergenic | 45.0% | |
| ACGCCTTCAAGCTGAAATGT+TGG | + | chr4.2:26363172-26363191 | MS.gene82385:intron | 45.0% | |
| ATCTGCGCCAACCATCAAAA+TGG | + | chr4.2:26363834-26363853 | MS.gene82385:intron | 45.0% | |
| ATGGGAAAGGATTTGGGCAA+AGG | - | chr4.2:26363505-26363524 | None:intergenic | 45.0% | |
| CAGTTCCGTTCCTGTTTGTT+AGG | + | chr4.2:26363953-26363972 | MS.gene82385:CDS | 45.0% | |
| GCACCAACATTTCAGCTTGA+AGG | - | chr4.2:26363178-26363197 | None:intergenic | 45.0% | |
| TCTCCCCTAACAAACAGGAA+CGG | - | chr4.2:26363961-26363980 | None:intergenic | 45.0% | |
| TGATTCCTGAACAAAGCCGA+AGG | + | chr4.2:26363527-26363546 | MS.gene82385:intron | 45.0% | |
| TGGGAAAGGATTTGGGCAAA+GGG | - | chr4.2:26363504-26363523 | None:intergenic | 45.0% | |
| TGTCTAGCAGCTGACATATG+TGG | - | chr4.2:26363052-26363071 | None:intergenic | 45.0% | |
| TTCAGGAATCACGATGGGAA+AGG | - | chr4.2:26363518-26363537 | None:intergenic | 45.0% | |
| ! | CATCAATGAGCACTGCATCT+GGG | - | chr4.2:26363606-26363625 | None:intergenic | 45.0% |
| ! | GCTTTGTTCAGGAATCACGA+TGG | - | chr4.2:26363524-26363543 | None:intergenic | 45.0% |
| ! | GTTCCGTTCCTGTTTGTTAG+GGG | + | chr4.2:26363955-26363974 | MS.gene82385:CDS | 45.0% |
| ! | TCATCAATGAGCACTGCATC+TGG | - | chr4.2:26363607-26363626 | None:intergenic | 45.0% |
| ! | TTCCTGTTTGTTAGGGGAGA+TGG | + | chr4.2:26363961-26363980 | MS.gene82385:CDS | 45.0% |
| !! | CACTCTCCCTATCAAGTTGA+AGG | + | chr4.2:26363449-26363468 | MS.gene82385:intron | 45.0% |
| !! | TATTGGTTTCTCCACCACTG+AGG | + | chr4.2:26363989-26364008 | MS.gene82385:CDS | 45.0% |
| !! | TGGTGCTACATAGGTCAAGA+GGG | + | chr4.2:26363192-26363211 | MS.gene82385:intron | 45.0% |
| !! | TTGGTGCTACATAGGTCAAG+AGG | + | chr4.2:26363191-26363210 | MS.gene82385:intron | 45.0% |
| !! | TTTCTCCTTCGGCTTTGTTC+AGG | - | chr4.2:26363535-26363554 | None:intergenic | 45.0% |
| !! | TTTTGATGGTTGGCGCAGAT+TGG | - | chr4.2:26363834-26363853 | None:intergenic | 45.0% |
| AGGAGAGGTAAGCGAAAGAG+AGG | + | chr4.2:26363224-26363243 | MS.gene82385:intron | 50.0% | |
| ATAGGTCAAGAGGGGTAGGA+AGG | + | chr4.2:26363201-26363220 | MS.gene82385:intron | 50.0% | |
| CTACATAGGTCAAGAGGGGT+AGG | + | chr4.2:26363197-26363216 | MS.gene82385:intron | 50.0% | |
| GCAAAGGGGATTGAAGTGGA+GGG | - | chr4.2:26363489-26363508 | None:intergenic | 50.0% | |
| GGGAAAGGATTTGGGCAAAG+GGG | - | chr4.2:26363503-26363522 | None:intergenic | 50.0% | |
| TAGGGGAGATGGCGTGATAT+TGG | + | chr4.2:26363972-26363991 | MS.gene82385:CDS | 50.0% | |
| TAGTCTCCAGCCTTGCTTTG+CGG | + | chr4.2:26363248-26363267 | MS.gene82385:intron | 50.0% | |
| TTGGGCAAAGGGGATTGAAG+TGG | - | chr4.2:26363493-26363512 | None:intergenic | 50.0% | |
| ! | GGTGCTACATAGGTCAAGAG+GGG | + | chr4.2:26363193-26363212 | MS.gene82385:intron | 50.0% |
| CGCCATCTCCCCTAACAAAC+AGG | - | chr4.2:26363966-26363985 | None:intergenic | 55.0% | |
| GGCAAAGGGGATTGAAGTGG+AGG | - | chr4.2:26363490-26363509 | None:intergenic | 55.0% | |
| !! | CGGAGGGCTTTCGAGTGTTT+TGG | + | chr4.2:26363329-26363348 | MS.gene82385:intron | 55.0% |
| AGAGGGGTAGGAAGGAGGAG+AGG | + | chr4.2:26363209-26363228 | MS.gene82385:intron | 60.0% | |
| GGTCAAGAGGGGTAGGAAGG+AGG | + | chr4.2:26363204-26363223 | MS.gene82385:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26362707 | 26364020 | 26362707 | ID=MS.gene82385 |
| chr4.2 | mRNA | 26362707 | 26364020 | 26362707 | ID=MS.gene82385.t1;Parent=MS.gene82385 |
| chr4.2 | exon | 26362707 | 26362802 | 26362707 | ID=MS.gene82385.t1.exon1;Parent=MS.gene82385.t1 |
| chr4.2 | CDS | 26362707 | 26362802 | 26362707 | ID=cds.MS.gene82385.t1;Parent=MS.gene82385.t1 |
| chr4.2 | exon | 26363940 | 26364020 | 26363940 | ID=MS.gene82385.t1.exon2;Parent=MS.gene82385.t1 |
| chr4.2 | CDS | 26363940 | 26364020 | 26363940 | ID=cds.MS.gene82385.t1;Parent=MS.gene82385.t1 |
| Gene Sequence |
| Protein sequence |
