Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82566.t1 | XP_003620485.1 | 97.6 | 167 | 4 | 0 | 1 | 167 | 1 | 167 | 8.00E-92 | 346.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82566.t1 | G7KN44 | 97.6 | 167 | 4 | 0 | 1 | 167 | 1 | 167 | 5.8e-92 | 346.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053427 | MS.gene82566 | 0.822075 | 2.88E-53 | -1.69E-46 |
| MS.gene05460 | MS.gene82566 | 0.844477 | 7.38E-59 | -1.69E-46 |
| MS.gene056059 | MS.gene82566 | 0.809463 | 1.87E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82566.t1 | MTR_6g084470 | 97.605 | 167 | 4 | 0 | 1 | 167 | 1 | 167 | 9.60e-121 | 337 |
| MS.gene82566.t1 | MTR_8g104210 | 63.793 | 174 | 49 | 7 | 3 | 167 | 4 | 172 | 4.07e-60 | 184 |
| MS.gene82566.t1 | MTR_8g104210 | 65.432 | 162 | 43 | 6 | 14 | 167 | 7 | 163 | 3.26e-58 | 179 |
| MS.gene82566.t1 | MTR_6g018430 | 42.286 | 175 | 86 | 6 | 1 | 167 | 1 | 168 | 2.37e-29 | 106 |
| MS.gene82566.t1 | MTR_1g110760 | 43.478 | 161 | 72 | 5 | 21 | 167 | 5 | 160 | 1.11e-21 | 86.3 |
| MS.gene82566.t1 | MTR_4g087400 | 37.267 | 161 | 67 | 7 | 1 | 153 | 1 | 135 | 2.07e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82566.t1 | AT5G16110 | 47.283 | 184 | 74 | 8 | 1 | 167 | 67 | 244 | 1.29e-32 | 116 |
| MS.gene82566.t1 | AT3G02555 | 46.893 | 177 | 69 | 6 | 1 | 167 | 1 | 162 | 1.50e-32 | 114 |
| MS.gene82566.t1 | AT3G02555 | 46.893 | 177 | 69 | 6 | 1 | 167 | 1 | 162 | 1.50e-32 | 114 |
| MS.gene82566.t1 | AT1G68490 | 39.773 | 176 | 87 | 5 | 5 | 167 | 14 | 183 | 5.86e-26 | 97.8 |
| MS.gene82566.t1 | AT1G13390 | 37.912 | 182 | 91 | 9 | 1 | 167 | 2 | 176 | 1.53e-19 | 81.3 |
| MS.gene82566.t1 | AT1G13390 | 37.912 | 182 | 91 | 9 | 1 | 167 | 2 | 176 | 1.53e-19 | 81.3 |
Find 47 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAAAATTTGTGGCCTAAA+AGG | 0.213971 | 6.3:-78775549 | None:intergenic |
| AATTGCTATAATGAAAGATT+TGG | 0.265335 | 6.3:+78776173 | MS.gene82566:CDS |
| AGACCAAATCGAATAGGTTT+AGG | 0.280991 | 6.3:-78775504 | None:intergenic |
| ATTGCTATAATGAAAGATTT+GGG | 0.288423 | 6.3:+78776174 | MS.gene82566:CDS |
| GAAAGATTTGGGGAGCAATT+AGG | 0.308640 | 6.3:+78776185 | MS.gene82566:CDS |
| GTGGCCTAAAAGGTCTGATA+TGG | 0.310493 | 6.3:-78775539 | None:intergenic |
| CAATCAGAGGTTGAAGATTC+AGG | 0.321864 | 6.3:+78775696 | MS.gene82566:CDS |
| AGGTTGAAGATTCAGGTGTT+AGG | 0.346664 | 6.3:+78775703 | MS.gene82566:CDS |
| GTGATCCAAGATGAGCAATT+TGG | 0.348409 | 6.3:+78776263 | MS.gene82566:CDS |
| TGCCCTAAACCTATTCGATT+TGG | 0.350421 | 6.3:+78775501 | MS.gene82566:CDS |
| GAAGAGGGTGCCATGGAAAA+TGG | 0.350699 | 6.3:-78776314 | None:intergenic |
| AGGTGTTAGGGAAGATCTTC+TGG | 0.363892 | 6.3:+78775716 | MS.gene82566:CDS |
| CATTGCCAAATTGCTCATCT+TGG | 0.383707 | 6.3:-78776268 | None:intergenic |
| GACCAAATCGAATAGGTTTA+GGG | 0.384037 | 6.3:-78775503 | None:intergenic |
| TCAAAATACCTCAAAATTTG+TGG | 0.413455 | 6.3:-78775558 | None:intergenic |
| TTTGTGGATCTCCACCTAGC+AGG | 0.415060 | 6.3:+78776228 | MS.gene82566:CDS |
| TGTGCTCCAATGAAGAAGTT+TGG | 0.423704 | 6.3:+78776359 | MS.gene82566:CDS |
| GGTTGAAGATTCAGGTGTTA+GGG | 0.425014 | 6.3:+78775704 | MS.gene82566:CDS |
| AGAGATGAGGATGGAATCTC+TGG | 0.450122 | 6.3:+78775476 | MS.gene82566:CDS |
| CAAGCTGCAGTGAGGATTGA+AGG | 0.451676 | 6.3:+78776389 | MS.gene82566:CDS |
| GTGTGACCAAACTTCTTCAT+TGG | 0.453010 | 6.3:-78776365 | None:intergenic |
| TTTGGCAATGGCAATGGTCA+TGG | 0.463708 | 6.3:+78776281 | MS.gene82566:CDS |
| TTCATCAGACCAAATCGAAT+AGG | 0.466136 | 6.3:-78775510 | None:intergenic |
| AATTGCTCATCTTGGATCAC+AGG | 0.470456 | 6.3:-78776260 | None:intergenic |
| TTCTGGATATCATTCTCCCC+AGG | 0.482476 | 6.3:+78775733 | MS.gene82566:CDS |
| TTGTGGATCTCCACCTAGCA+GGG | 0.495640 | 6.3:+78776229 | MS.gene82566:CDS |
| TTGATTGTCTCAATAGGGAT+AGG | 0.498503 | 6.3:+78776414 | MS.gene82566:CDS |
| CATCTCTTCACAGCTTCTCA+TGG | 0.503294 | 6.3:-78775460 | None:intergenic |
| TCACCATCTTCCTCAGCTAG+AGG | 0.554835 | 6.3:+78776335 | MS.gene82566:CDS |
| TTGGCAATGGCAATGGTCAT+GGG | 0.558206 | 6.3:+78776282 | MS.gene82566:CDS |
| GAGCAATTTGGCAATGGCAA+TGG | 0.565272 | 6.3:+78776275 | MS.gene82566:CDS |
| CAACCTCTAGCTGAGGAAGA+TGG | 0.571591 | 6.3:-78776338 | None:intergenic |
| AAGCTGTGAAGAGATGAGGA+TGG | 0.577435 | 6.3:+78775467 | MS.gene82566:CDS |
| AGCTGAGGAAGATGGTGAAG+AGG | 0.581033 | 6.3:-78776330 | None:intergenic |
| AGATGGTGAAGAGGGTGCCA+TGG | 0.590017 | 6.3:-78776321 | None:intergenic |
| ACAGGGTTTGAAGCCCTGCT+AGG | 0.591659 | 6.3:-78776242 | None:intergenic |
| ATTGCTCATCTTGGATCACA+GGG | 0.597595 | 6.3:-78776259 | None:intergenic |
| CAAGATGAGCAATTTGGCAA+TGG | 0.600303 | 6.3:+78776269 | MS.gene82566:CDS |
| GGGTTTGAAGCCCTGCTAGG+TGG | 0.606726 | 6.3:-78776239 | None:intergenic |
| GCTGAGGAAGATGGTGAAGA+GGG | 0.607679 | 6.3:-78776329 | None:intergenic |
| TTGCTATAATGAAAGATTTG+GGG | 0.611759 | 6.3:+78776175 | MS.gene82566:CDS |
| TCTGGATATCATTCTCCCCA+GGG | 0.618574 | 6.3:+78775734 | MS.gene82566:CDS |
| AATAATCAGCTATCAATCAG+AGG | 0.653539 | 6.3:+78775683 | MS.gene82566:intron |
| TGGAGCACAACCTCTAGCTG+AGG | 0.654183 | 6.3:-78776345 | None:intergenic |
| TGAGAAGCTGTGAAGAGATG+AGG | 0.665577 | 6.3:+78775463 | MS.gene82566:CDS |
| GTCACACACAAGCTGCAGTG+AGG | 0.704483 | 6.3:+78776381 | MS.gene82566:CDS |
| TGTGAAAAGATGAATCACTG+TGG | 0.839717 | 6.3:+78775420 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAATAATTAAATATAAAATT+AGG | - | chr6.3:78775588-78775607 | None:intergenic | 0.0% |
| !!! | TCTTAATTTTTCATGAAAAA+AGG | + | chr6.3:78775774-78775793 | MS.gene82566:intron | 15.0% |
| !! | AATTGCTATAATGAAAGATT+TGG | + | chr6.3:78776173-78776192 | MS.gene82566:CDS | 20.0% |
| !! | ATTGCTATAATGAAAGATTT+GGG | + | chr6.3:78776174-78776193 | MS.gene82566:CDS | 20.0% |
| !!! | TCAAATAAAATACCATGTTT+TGG | + | chr6.3:78775809-78775828 | MS.gene82566:intron | 20.0% |
| ! | ATGGTTAATGAACTTCATTA+AGG | + | chr6.3:78775932-78775951 | MS.gene82566:intron | 25.0% |
| ! | CCGAAATAAAGTAATAAAGT+CGG | + | chr6.3:78775997-78776016 | MS.gene82566:intron | 25.0% |
| ! | TCAAAATACCTCAAAATTTG+TGG | - | chr6.3:78775561-78775580 | None:intergenic | 25.0% |
| ! | TGTCTAATCTAACAATGATT+TGG | + | chr6.3:78776138-78776157 | MS.gene82566:intron | 25.0% |
| ! | TTGCTATAATGAAAGATTTG+GGG | + | chr6.3:78776175-78776194 | MS.gene82566:CDS | 25.0% |
| !! | CCGACTTTATTACTTTATTT+CGG | - | chr6.3:78776000-78776019 | None:intergenic | 25.0% |
| AATAATCAGCTATCAATCAG+AGG | + | chr6.3:78775683-78775702 | MS.gene82566:intron | 30.0% | |
| ATGATTGATGCACAATTACT+AGG | + | chr6.3:78775834-78775853 | MS.gene82566:intron | 30.0% | |
| ATTCTCATGGAAAATCATCA+TGG | - | chr6.3:78776050-78776069 | None:intergenic | 30.0% | |
| CATCAATCATAACCAAAACA+TGG | - | chr6.3:78775824-78775843 | None:intergenic | 30.0% | |
| TGATTGATGCACAATTACTA+GGG | + | chr6.3:78775835-78775854 | MS.gene82566:intron | 30.0% | |
| TTGACATAGAAATTTACCCT+GGG | - | chr6.3:78775753-78775772 | None:intergenic | 30.0% | |
| TTTGACATAGAAATTTACCC+TGG | - | chr6.3:78775754-78775773 | None:intergenic | 30.0% | |
| ! | ATCACTTGATTTGACTCAAA+TGG | + | chr6.3:78775913-78775932 | MS.gene82566:intron | 30.0% |
| !! | AACAATGATTTGGATTTTGC+AGG | + | chr6.3:78776148-78776167 | MS.gene82566:intron | 30.0% |
| !! | ACAATGATTTGGATTTTGCA+GGG | + | chr6.3:78776149-78776168 | MS.gene82566:intron | 30.0% |
| !! | GAATTTTAGTCCATTTTCCA+TGG | + | chr6.3:78776304-78776323 | MS.gene82566:CDS | 30.0% |
| !!! | AAGGTTTTGATTGTCTCAAT+AGG | + | chr6.3:78776408-78776427 | MS.gene82566:CDS | 30.0% |
| !!! | AGGTTTTGATTGTCTCAATA+GGG | + | chr6.3:78776409-78776428 | MS.gene82566:CDS | 30.0% |
| AGACCAAATCGAATAGGTTT+AGG | - | chr6.3:78775507-78775526 | None:intergenic | 35.0% | |
| CTCAAAATTTGTGGCCTAAA+AGG | - | chr6.3:78775552-78775571 | None:intergenic | 35.0% | |
| GACCAAATCGAATAGGTTTA+GGG | - | chr6.3:78775506-78775525 | None:intergenic | 35.0% | |
| GGAAAATCATCATGGTAATC+AGG | - | chr6.3:78776042-78776061 | None:intergenic | 35.0% | |
| TAAGCTGGAAAAAAGAAGCT+TGG | + | chr6.3:78776079-78776098 | MS.gene82566:intron | 35.0% | |
| TGACATAGAAATTTACCCTG+GGG | - | chr6.3:78775752-78775771 | None:intergenic | 35.0% | |
| TTCATCAGACCAAATCGAAT+AGG | - | chr6.3:78775513-78775532 | None:intergenic | 35.0% | |
| TTGATTGTCTCAATAGGGAT+AGG | + | chr6.3:78776414-78776433 | MS.gene82566:CDS | 35.0% | |
| ! | ATTACTTTATTTCGGCCAGA+CGG | - | chr6.3:78775992-78776011 | None:intergenic | 35.0% |
| ! | CTTTTAGGCCACAAATTTTG+AGG | + | chr6.3:78775550-78775569 | MS.gene82566:CDS | 35.0% |
| ! | GAAACCATATCAGACCTTTT+AGG | + | chr6.3:78775535-78775554 | MS.gene82566:CDS | 35.0% |
| ! | GATTTTCCATGAGAATCAGA+GGG | + | chr6.3:78776054-78776073 | MS.gene82566:intron | 35.0% |
| ! | TGATTTTCCATGAGAATCAG+AGG | + | chr6.3:78776053-78776072 | MS.gene82566:intron | 35.0% |
| AATTGCTCATCTTGGATCAC+AGG | - | chr6.3:78776263-78776282 | None:intergenic | 40.0% | |
| AGGTTGAAGATTCAGGTGTT+AGG | + | chr6.3:78775703-78775722 | MS.gene82566:CDS | 40.0% | |
| ATTGCTCATCTTGGATCACA+GGG | - | chr6.3:78776262-78776281 | None:intergenic | 40.0% | |
| CAAGATGAGCAATTTGGCAA+TGG | + | chr6.3:78776269-78776288 | MS.gene82566:CDS | 40.0% | |
| CAATCAGAGGTTGAAGATTC+AGG | + | chr6.3:78775696-78775715 | MS.gene82566:CDS | 40.0% | |
| CATTGCCAAATTGCTCATCT+TGG | - | chr6.3:78776271-78776290 | None:intergenic | 40.0% | |
| CTTAAACCCTCTGATTCTCA+TGG | - | chr6.3:78776063-78776082 | None:intergenic | 40.0% | |
| GAAAGATTTGGGGAGCAATT+AGG | + | chr6.3:78776185-78776204 | MS.gene82566:CDS | 40.0% | |
| GATCCACAAAAAAATGGTGG+TGG | - | chr6.3:78776218-78776237 | None:intergenic | 40.0% | |
| GGAGATCCACAAAAAAATGG+TGG | - | chr6.3:78776221-78776240 | None:intergenic | 40.0% | |
| GGTGGAGATCCACAAAAAAA+TGG | - | chr6.3:78776224-78776243 | None:intergenic | 40.0% | |
| GGTTGAAGATTCAGGTGTTA+GGG | + | chr6.3:78775704-78775723 | MS.gene82566:CDS | 40.0% | |
| GTGATCCAAGATGAGCAATT+TGG | + | chr6.3:78776263-78776282 | MS.gene82566:CDS | 40.0% | |
| GTGTGACCAAACTTCTTCAT+TGG | - | chr6.3:78776368-78776387 | None:intergenic | 40.0% | |
| TGCCCTAAACCTATTCGATT+TGG | + | chr6.3:78775501-78775520 | MS.gene82566:CDS | 40.0% | |
| TGTGCTCCAATGAAGAAGTT+TGG | + | chr6.3:78776359-78776378 | MS.gene82566:CDS | 40.0% | |
| !!! | TCACCACCACCATTTTTTTG+TGG | + | chr6.3:78776212-78776231 | MS.gene82566:CDS | 40.0% |
| AAGCTGTGAAGAGATGAGGA+TGG | + | chr6.3:78775467-78775486 | MS.gene82566:CDS | 45.0% | |
| AGAGATGAGGATGGAATCTC+TGG | + | chr6.3:78775476-78775495 | MS.gene82566:CDS | 45.0% | |
| AGGTGTTAGGGAAGATCTTC+TGG | + | chr6.3:78775716-78775735 | MS.gene82566:CDS | 45.0% | |
| CATCTCTTCACAGCTTCTCA+TGG | - | chr6.3:78775463-78775482 | None:intergenic | 45.0% | |
| GAGAATCAGAGGGTTTAAGC+TGG | + | chr6.3:78776064-78776083 | MS.gene82566:intron | 45.0% | |
| GAGCAATTTGGCAATGGCAA+TGG | + | chr6.3:78776275-78776294 | MS.gene82566:CDS | 45.0% | |
| GTGGCCTAAAAGGTCTGATA+TGG | - | chr6.3:78775542-78775561 | None:intergenic | 45.0% | |
| TCTGGATATCATTCTCCCCA+GGG | + | chr6.3:78775734-78775753 | MS.gene82566:CDS | 45.0% | |
| TGAGAAGCTGTGAAGAGATG+AGG | + | chr6.3:78775463-78775482 | MS.gene82566:CDS | 45.0% | |
| TTCTGGATATCATTCTCCCC+AGG | + | chr6.3:78775733-78775752 | MS.gene82566:CDS | 45.0% | |
| TTGGCAATGGCAATGGTCAT+GGG | + | chr6.3:78776282-78776301 | MS.gene82566:CDS | 45.0% | |
| TTTGGCAATGGCAATGGTCA+TGG | + | chr6.3:78776281-78776300 | MS.gene82566:CDS | 45.0% | |
| !! | TTGCCGACTTTATTACCGTC+TGG | + | chr6.3:78775974-78775993 | MS.gene82566:intron | 45.0% |
| AGCTGAGGAAGATGGTGAAG+AGG | - | chr6.3:78776333-78776352 | None:intergenic | 50.0% | |
| CAACCTCTAGCTGAGGAAGA+TGG | - | chr6.3:78776341-78776360 | None:intergenic | 50.0% | |
| CAAGCTGCAGTGAGGATTGA+AGG | + | chr6.3:78776389-78776408 | MS.gene82566:CDS | 50.0% | |
| CGGCCAGACGGTAATAAAGT+CGG | - | chr6.3:78775980-78775999 | None:intergenic | 50.0% | |
| GCTGAGGAAGATGGTGAAGA+GGG | - | chr6.3:78776332-78776351 | None:intergenic | 50.0% | |
| TCACCATCTTCCTCAGCTAG+AGG | + | chr6.3:78776335-78776354 | MS.gene82566:CDS | 50.0% | |
| TTGTGGATCTCCACCTAGCA+GGG | + | chr6.3:78776229-78776248 | MS.gene82566:CDS | 50.0% | |
| TTTGTGGATCTCCACCTAGC+AGG | + | chr6.3:78776228-78776247 | MS.gene82566:CDS | 50.0% | |
| !! | GAAGAGGGTGCCATGGAAAA+TGG | - | chr6.3:78776317-78776336 | None:intergenic | 50.0% |
| GTCACACACAAGCTGCAGTG+AGG | + | chr6.3:78776381-78776400 | MS.gene82566:CDS | 55.0% | |
| TGGAGCACAACCTCTAGCTG+AGG | - | chr6.3:78776348-78776367 | None:intergenic | 55.0% | |
| ! | ACAGGGTTTGAAGCCCTGCT+AGG | - | chr6.3:78776245-78776264 | None:intergenic | 55.0% |
| !! | AGATGGTGAAGAGGGTGCCA+TGG | - | chr6.3:78776324-78776343 | None:intergenic | 55.0% |
| ! | GGGTTTGAAGCCCTGCTAGG+TGG | - | chr6.3:78776242-78776261 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 78775429 | 78776466 | 78775429 | ID=MS.gene82566 |
| chr6.3 | mRNA | 78775429 | 78776466 | 78775429 | ID=MS.gene82566.t1;Parent=MS.gene82566 |
| chr6.3 | exon | 78775429 | 78775571 | 78775429 | ID=MS.gene82566.t1.exon1;Parent=MS.gene82566.t1 |
| chr6.3 | CDS | 78775429 | 78775571 | 78775429 | ID=cds.MS.gene82566.t1;Parent=MS.gene82566.t1 |
| chr6.3 | exon | 78775692 | 78775755 | 78775692 | ID=MS.gene82566.t1.exon2;Parent=MS.gene82566.t1 |
| chr6.3 | CDS | 78775692 | 78775755 | 78775692 | ID=cds.MS.gene82566.t1;Parent=MS.gene82566.t1 |
| chr6.3 | exon | 78776170 | 78776466 | 78776170 | ID=MS.gene82566.t1.exon3;Parent=MS.gene82566.t1 |
| chr6.3 | CDS | 78776170 | 78776466 | 78776170 | ID=cds.MS.gene82566.t1;Parent=MS.gene82566.t1 |
| Gene Sequence |
| Protein sequence |
