Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82804.t1 | XP_028762943.1 | 62 | 350 | 79 | 3 | 1 | 298 | 1 | 348 | 3.90E-113 | 417.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82804.t1 | A0A1U8KII2 | 57.1 | 336 | 92 | 3 | 1 | 284 | 1 | 336 | 9.3e-101 | 376.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059272 | MS.gene82804 | 0.906577 | 1.28E-80 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82804.t1 | MTR_7g062720 | 65.909 | 132 | 45 | 0 | 1 | 132 | 1 | 132 | 2.25e-60 | 190 |
| MS.gene82804.t1 | MTR_5g061150 | 62.992 | 127 | 47 | 0 | 20 | 146 | 10 | 136 | 6.08e-49 | 159 |
| MS.gene82804.t1 | MTR_4g037355 | 50.000 | 116 | 17 | 2 | 151 | 225 | 1 | 116 | 1.88e-26 | 101 |
| MS.gene82804.t1 | MTR_4g112730 | 75.410 | 61 | 15 | 0 | 138 | 198 | 1 | 61 | 7.35e-25 | 95.9 |
| MS.gene82804.t1 | MTR_1g036690 | 58.537 | 82 | 27 | 3 | 218 | 298 | 5 | 80 | 2.67e-23 | 91.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 46 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGAGGCTCTTAATATTTC+TGG | 0.080746 | 6.1:+585887 | MS.gene82804:CDS |
| TTCGGCATTTAAATGAATTT+TGG | 0.166319 | 6.1:-585939 | None:intergenic |
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 6.1:+586788 | MS.gene82804:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 6.1:+586780 | MS.gene82804:CDS |
| TAGAAGATCCAAAAGATCTT+TGG | 0.269782 | 6.1:+586077 | MS.gene82804:CDS |
| TATTTCTGGAAATAATTATC+TGG | 0.282933 | 6.1:+585901 | MS.gene82804:CDS |
| ATGGTTGTATTTGCGTTTAC+AGG | 0.299146 | 6.1:+586162 | MS.gene82804:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 6.1:-586942 | None:intergenic |
| TAAAGATCAACAAGATGTTT+TGG | 0.333763 | 6.1:-586757 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 6.1:+586840 | MS.gene82804:CDS |
| CTGGAAATAATTATCTGGCT+TGG | 0.347827 | 6.1:+585906 | MS.gene82804:CDS |
| TGGAAATAATTATCTGGCTT+GGG | 0.361577 | 6.1:+585907 | MS.gene82804:CDS |
| TTTGGGAAAATTTAAAAGAA+AGG | 0.385472 | 6.1:+586095 | MS.gene82804:CDS |
| AACCATTCGTTACGAGCATT+AGG | 0.390171 | 6.1:-586145 | None:intergenic |
| AGAAGATCCAAAAGATCTTT+GGG | 0.393236 | 6.1:+586078 | MS.gene82804:CDS |
| GAAAATGATGAAGATGATTA+TGG | 0.394855 | 6.1:+586832 | MS.gene82804:CDS |
| TGCTATCGTTGTGGAGGAAA+AGG | 0.414829 | 6.1:+586709 | MS.gene82804:CDS |
| CCTTCGTCACCACCTTCATA+AGG | 0.433246 | 6.1:+586030 | MS.gene82804:CDS |
| CAAAACAACTGAACAACAAA+AGG | 0.454927 | 6.1:+585991 | MS.gene82804:CDS |
| AGAAATCAGCAGCATCTAAG+TGG | 0.464577 | 6.1:-586870 | None:intergenic |
| AATGGTATCTCCATGCCCTT+CGG | 0.470113 | 6.1:-585957 | None:intergenic |
| AATGTTGTAAAGAATGATAT+TGG | 0.471241 | 6.1:+586658 | MS.gene82804:CDS |
| ATTGACACTGTGTGGAGAAA+AGG | 0.483584 | 6.1:+586237 | MS.gene82804:CDS |
| GCAGCAACAATACCGCGAAA+AGG | 0.486558 | 6.1:+586321 | MS.gene82804:CDS |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 6.1:+586789 | MS.gene82804:CDS |
| GCTACATTCACTTCTGGGAA+TGG | 0.488554 | 6.1:-586445 | None:intergenic |
| TACATTAGATGCATGAAAAG+TGG | 0.490606 | 6.1:-586293 | None:intergenic |
| CAGCAACAATACCGCGAAAA+GGG | 0.491478 | 6.1:+586322 | MS.gene82804:CDS |
| CATTTAAATGCCGAAGGGCA+TGG | 0.494088 | 6.1:+585947 | MS.gene82804:CDS |
| GTTGTGGAGGAAAAGGTCAT+TGG | 0.504176 | 6.1:+586716 | MS.gene82804:CDS |
| CATGGAGATACCATTAAAGA+AGG | 0.521442 | 6.1:+585965 | MS.gene82804:CDS |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 6.1:+586924 | MS.gene82804:CDS |
| AAAATTCATTTAAATGCCGA+AGG | 0.537172 | 6.1:+585941 | MS.gene82804:CDS |
| TTCCTAATGCTCGTAACGAA+TGG | 0.575736 | 6.1:+586143 | MS.gene82804:CDS |
| AACTGAACAACAAAAGGCTA+AGG | 0.582581 | 6.1:+585997 | MS.gene82804:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 6.1:+586700 | MS.gene82804:CDS |
| ATTCGTTACGAGCATTAGGA+AGG | 0.589689 | 6.1:-586141 | None:intergenic |
| CCTTATGAAGGTGGTGACGA+AGG | 0.598722 | 6.1:-586030 | None:intergenic |
| AGTATTTGCTATCGTTGTGG+AGG | 0.599695 | 6.1:+586703 | MS.gene82804:CDS |
| AATCACACGACTCGTCCCAC+TGG | 0.600994 | 6.1:+586415 | MS.gene82804:CDS |
| GAAATCAGCAGCATCTAAGT+GGG | 0.621669 | 6.1:-586869 | None:intergenic |
| AAATTCATTTAAATGCCGAA+GGG | 0.631745 | 6.1:+585942 | MS.gene82804:CDS |
| GTGGTGACGAAGGAATATCA+TGG | 0.663314 | 6.1:-586020 | None:intergenic |
| ACTTCTCTATTGACACTGTG+TGG | 0.668278 | 6.1:+586229 | MS.gene82804:CDS |
| TGGGAATGGAGCACTACCAG+TGG | 0.672955 | 6.1:-586431 | None:intergenic |
| GGGAATGGAGCACTACCAGT+GGG | 0.676344 | 6.1:-586430 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | + | chr6.1:586658-586677 | MS.gene82804:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | + | chr6.1:586789-586808 | MS.gene82804:CDS | 20.0% |
| !! | TTTGGGAAAATTTAAAAGAA+AGG | + | chr6.1:586095-586114 | MS.gene82804:CDS | 20.0% |
| !!! | TATTTCTGGAAATAATTATC+TGG | + | chr6.1:585901-585920 | MS.gene82804:CDS | 20.0% |
| ! | AAAATTCATTTAAATGCCGA+AGG | + | chr6.1:585941-585960 | MS.gene82804:CDS | 25.0% |
| ! | AAATTCATTTAAATGCCGAA+GGG | + | chr6.1:585942-585961 | MS.gene82804:CDS | 25.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | + | chr6.1:586788-586807 | MS.gene82804:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | + | chr6.1:586832-586851 | MS.gene82804:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | + | chr6.1:586840-586859 | MS.gene82804:CDS | 25.0% |
| !! | AAATTTTCCCAAAGATCTTT+TGG | - | chr6.1:586088-586107 | None:intergenic | 25.0% |
| !! | ATCATTTTCACATGCAAAAT+GGG | - | chr6.1:586821-586840 | None:intergenic | 25.0% |
| !! | TAAAGATCAACAAGATGTTT+TGG | - | chr6.1:586760-586779 | None:intergenic | 25.0% |
| !! | TTACAACATTTTTCATCTTC+TGG | - | chr6.1:586648-586667 | None:intergenic | 25.0% |
| !! | TTCGGCATTTAAATGAATTT+TGG | - | chr6.1:585942-585961 | None:intergenic | 25.0% |
| !!! | CATTTTTTAGATCCTTATGA+AGG | - | chr6.1:586045-586064 | None:intergenic | 25.0% |
| !!! | GAGAAAATGTGTTTTTGTTT+TGG | - | chr6.1:586624-586643 | None:intergenic | 25.0% |
| !!! | TAAATGAATTTTGGCATCAA+GGG | - | chr6.1:585933-585952 | None:intergenic | 25.0% |
| !!! | TGTTTTGTTTCCTTCTTTAA+TGG | - | chr6.1:585978-585997 | None:intergenic | 25.0% |
| !!! | TTAAATGAATTTTGGCATCA+AGG | - | chr6.1:585934-585953 | None:intergenic | 25.0% |
| AGAAGATCCAAAAGATCTTT+GGG | + | chr6.1:586078-586097 | MS.gene82804:CDS | 30.0% | |
| CAAAACAACTGAACAACAAA+AGG | + | chr6.1:585991-586010 | MS.gene82804:CDS | 30.0% | |
| CATTAGAGATGAAAATATCC+AGG | + | chr6.1:586924-586943 | MS.gene82804:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | + | chr6.1:586780-586799 | MS.gene82804:CDS | 30.0% | |
| TACATTAGATGCATGAAAAG+TGG | - | chr6.1:586296-586315 | None:intergenic | 30.0% | |
| TAGAAGATCCAAAAGATCTT+TGG | + | chr6.1:586077-586096 | MS.gene82804:CDS | 30.0% | |
| ! | AAGATCTTTTGGATCTTCTA+TGG | - | chr6.1:586077-586096 | None:intergenic | 30.0% |
| ! | AGATGATCAATCTTTTCATC+AGG | - | chr6.1:586904-586923 | None:intergenic | 30.0% |
| ! | CATCATTTTCACATGCAAAA+TGG | - | chr6.1:586822-586841 | None:intergenic | 30.0% |
| ! | TGGAAATAATTATCTGGCTT+GGG | + | chr6.1:585907-585926 | MS.gene82804:CDS | 30.0% |
| ! | TTTGAGGCTCTTAATATTTC+TGG | + | chr6.1:585887-585906 | MS.gene82804:CDS | 30.0% |
| ! | TTTTTAGATCCTTATGAAGG+TGG | - | chr6.1:586042-586061 | None:intergenic | 30.0% |
| !! | GGTTTTAATCGTAATCACAA+TGG | + | chr6.1:586574-586593 | MS.gene82804:intron | 30.0% |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | - | chr6.1:586783-586802 | None:intergenic | 30.0% |
| AACTGAACAACAAAAGGCTA+AGG | + | chr6.1:585997-586016 | MS.gene82804:CDS | 35.0% | |
| ATGGTTGTATTTGCGTTTAC+AGG | + | chr6.1:586162-586181 | MS.gene82804:CDS | 35.0% | |
| CATGGAGATACCATTAAAGA+AGG | + | chr6.1:585965-585984 | MS.gene82804:CDS | 35.0% | |
| CTGGAAATAATTATCTGGCT+TGG | + | chr6.1:585906-585925 | MS.gene82804:CDS | 35.0% | |
| ! | CCTCAAAATCAAGTTTTGCA+AGG | - | chr6.1:585874-585893 | None:intergenic | 35.0% |
| ! | CCTTGCAAAACTTGATTTTG+AGG | + | chr6.1:585871-585890 | MS.gene82804:CDS | 35.0% |
| ! | CTTTTGGATCTTCTATGGTA+AGG | - | chr6.1:586072-586091 | None:intergenic | 35.0% |
| !!! | ACCTTTTAAAACCCTTTTCG+CGG | - | chr6.1:586336-586355 | None:intergenic | 35.0% |
| AACCATTCGTTACGAGCATT+AGG | - | chr6.1:586148-586167 | None:intergenic | 40.0% | |
| ACTTCTCTATTGACACTGTG+TGG | + | chr6.1:586229-586248 | MS.gene82804:CDS | 40.0% | |
| AGAAATCAGCAGCATCTAAG+TGG | - | chr6.1:586873-586892 | None:intergenic | 40.0% | |
| ATTCGTTACGAGCATTAGGA+AGG | - | chr6.1:586144-586163 | None:intergenic | 40.0% | |
| ATTGACACTGTGTGGAGAAA+AGG | + | chr6.1:586237-586256 | MS.gene82804:CDS | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | - | chr6.1:586872-586891 | None:intergenic | 40.0% | |
| TTCCTAATGCTCGTAACGAA+TGG | + | chr6.1:586143-586162 | MS.gene82804:CDS | 40.0% | |
| ! | GAAGGATGACAGTTTTCTGA+TGG | - | chr6.1:586126-586145 | None:intergenic | 40.0% |
| !! | AGTATTTGCTATCGTTGTGG+AGG | + | chr6.1:586703-586722 | MS.gene82804:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | + | chr6.1:586700-586719 | MS.gene82804:CDS | 40.0% |
| !!! | ACCGCGAAAAGGGTTTTAAA+AGG | + | chr6.1:586332-586351 | MS.gene82804:CDS | 40.0% |
| AATGGTATCTCCATGCCCTT+CGG | - | chr6.1:585960-585979 | None:intergenic | 45.0% | |
| CAGCAACAATACCGCGAAAA+GGG | + | chr6.1:586322-586341 | MS.gene82804:CDS | 45.0% | |
| CATTTAAATGCCGAAGGGCA+TGG | + | chr6.1:585947-585966 | MS.gene82804:CDS | 45.0% | |
| GATCACTATGGACAAACTCG+TGG | + | chr6.1:586475-586494 | MS.gene82804:intron | 45.0% | |
| GCGTTGCTACATTCACTTCT+GGG | - | chr6.1:586453-586472 | None:intergenic | 45.0% | |
| GTAGCAACGCATGATCACTA+TGG | + | chr6.1:586463-586482 | MS.gene82804:intron | 45.0% | |
| GTTGTGGAGGAAAAGGTCAT+TGG | + | chr6.1:586716-586735 | MS.gene82804:CDS | 45.0% | |
| TGCGTTGCTACATTCACTTC+TGG | - | chr6.1:586454-586473 | None:intergenic | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | + | chr6.1:586709-586728 | MS.gene82804:CDS | 45.0% | |
| ! | GCTACATTCACTTCTGGGAA+TGG | - | chr6.1:586448-586467 | None:intergenic | 45.0% |
| ! | GTGGTGACGAAGGAATATCA+TGG | - | chr6.1:586023-586042 | None:intergenic | 45.0% |
| !!! | AAGATGTTTTGGCGTACGAC+AGG | - | chr6.1:586749-586768 | None:intergenic | 45.0% |
| ACGTGGTCGTGGTTATGGAA+GGG | + | chr6.1:586540-586559 | MS.gene82804:intron | 50.0% | |
| CCTTCGTCACCACCTTCATA+AGG | + | chr6.1:586030-586049 | MS.gene82804:CDS | 50.0% | |
| GCAGCAACAATACCGCGAAA+AGG | + | chr6.1:586321-586340 | MS.gene82804:CDS | 50.0% | |
| TATGGACAAACTCGTGGTCG+TGG | + | chr6.1:586481-586500 | MS.gene82804:intron | 50.0% | |
| ! | CCTTATGAAGGTGGTGACGA+AGG | - | chr6.1:586033-586052 | None:intergenic | 50.0% |
| ! | TATGGAAGGGGACGTGGTTT+TGG | + | chr6.1:586553-586572 | MS.gene82804:intron | 50.0% |
| AATCACACGACTCGTCCCAC+TGG | + | chr6.1:586415-586434 | MS.gene82804:CDS | 55.0% | |
| CACGTCCACGACCATAACCA+TGG | - | chr6.1:586513-586532 | None:intergenic | 55.0% | |
| CATGGTTATGGTCGTGGACG+TGG | + | chr6.1:586511-586530 | MS.gene82804:intron | 55.0% | |
| CGTGGTCGTGGTTATGGAAG+GGG | + | chr6.1:586541-586560 | MS.gene82804:intron | 55.0% | |
| GACGTGGTCGTGGTTATGGA+AGG | + | chr6.1:586539-586558 | MS.gene82804:intron | 55.0% | |
| ! | GGGAATGGAGCACTACCAGT+GGG | - | chr6.1:586433-586452 | None:intergenic | 55.0% |
| ! | TGGGAATGGAGCACTACCAG+TGG | - | chr6.1:586434-586453 | None:intergenic | 55.0% |
| CAAACTCGTGGTCGTGGTCG+TGG | + | chr6.1:586487-586506 | MS.gene82804:intron | 60.0% | |
| CGTGGACGTGGTCGTGGTTA+TGG | + | chr6.1:586535-586554 | MS.gene82804:intron | 60.0% | |
| CGTGGCCATGGTTATGGTCG+TGG | + | chr6.1:586505-586524 | MS.gene82804:intron | 60.0% | |
| CGTGGTCGTGGCCATGGTTA+TGG | + | chr6.1:586499-586518 | MS.gene82804:intron | 60.0% | |
| CGTGGTTATGGAAGGGGACG+TGG | + | chr6.1:586547-586566 | MS.gene82804:intron | 60.0% | |
| TATGGTCGTGGACGTGGACG+TGG | + | chr6.1:586517-586536 | MS.gene82804:intron | 60.0% | |
| CGTGGACGTGGACGTGGACG+TGG | + | chr6.1:586523-586542 | MS.gene82804:intron | 70.0% | |
| CGTGGACGTGGACGTGGTCG+TGG | + | chr6.1:586529-586548 | MS.gene82804:intron | 70.0% | |
| CGTGGTCGTGGTCGTGGCCA+TGG | + | chr6.1:586493-586512 | MS.gene82804:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 585863 | 586951 | 585863 | ID=MS.gene82804 |
| chr6.1 | mRNA | 585863 | 586951 | 585863 | ID=MS.gene82804.t1;Parent=MS.gene82804 |
| chr6.1 | exon | 585863 | 586456 | 585863 | ID=MS.gene82804.t1.exon1;Parent=MS.gene82804.t1 |
| chr6.1 | CDS | 585863 | 586456 | 585863 | ID=cds.MS.gene82804.t1;Parent=MS.gene82804.t1 |
| chr6.1 | exon | 586649 | 586951 | 586649 | ID=MS.gene82804.t1.exon2;Parent=MS.gene82804.t1 |
| chr6.1 | CDS | 586649 | 586951 | 586649 | ID=cds.MS.gene82804.t1;Parent=MS.gene82804.t1 |
| Gene Sequence |
| Protein sequence |
