Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82966.t1 | XP_013446290.1 | 80 | 699 | 32 | 1 | 3 | 593 | 4 | 702 | 1.30E-293 | 1018.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82966.t1 | Q9LYG9 | 47.3 | 482 | 239 | 6 | 112 | 583 | 258 | 734 | 3.2e-107 | 390.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82966.t1 | A0A072TSB8 | 80.0 | 699 | 32 | 1 | 3 | 593 | 4 | 702 | 9.3e-294 | 1018.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056538 | MS.gene82966 | 0.874993 | 4.37E-68 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82966.t1 | MTR_8g071250 | 98.967 | 484 | 5 | 0 | 110 | 593 | 219 | 702 | 0.0 | 993 |
| MS.gene82966.t1 | MTR_8g071250 | 90.826 | 109 | 10 | 0 | 3 | 111 | 4 | 112 | 1.28e-60 | 214 |
| MS.gene82966.t1 | MTR_2g041220 | 51.215 | 494 | 213 | 7 | 110 | 586 | 271 | 753 | 1.55e-170 | 503 |
| MS.gene82966.t1 | MTR_3g104940 | 47.228 | 487 | 232 | 11 | 110 | 582 | 289 | 764 | 1.42e-141 | 429 |
| MS.gene82966.t1 | MTR_3g104920 | 47.648 | 489 | 221 | 10 | 110 | 579 | 254 | 726 | 9.14e-141 | 426 |
| MS.gene82966.t1 | MTR_3g104920 | 47.648 | 489 | 221 | 10 | 110 | 579 | 266 | 738 | 1.60e-140 | 426 |
| MS.gene82966.t1 | MTR_3g104990 | 48.148 | 486 | 229 | 10 | 110 | 582 | 288 | 763 | 1.91e-138 | 421 |
| MS.gene82966.t1 | MTR_1g113850 | 41.243 | 531 | 257 | 7 | 110 | 589 | 391 | 917 | 3.44e-122 | 383 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82966.t1 | AT5G12080 | 46.888 | 482 | 241 | 7 | 112 | 583 | 258 | 734 | 8.38e-148 | 444 |
| MS.gene82966.t1 | AT5G12080 | 46.888 | 482 | 241 | 7 | 112 | 583 | 258 | 734 | 8.38e-148 | 444 |
| MS.gene82966.t1 | AT5G12080 | 46.888 | 482 | 241 | 7 | 112 | 583 | 258 | 734 | 8.38e-148 | 444 |
| MS.gene82966.t1 | AT5G19520 | 44.167 | 480 | 248 | 11 | 111 | 580 | 270 | 739 | 5.11e-130 | 398 |
| MS.gene82966.t1 | AT5G19520 | 44.167 | 480 | 248 | 11 | 111 | 580 | 270 | 739 | 5.11e-130 | 398 |
| MS.gene82966.t1 | AT1G53470 | 42.136 | 515 | 254 | 9 | 110 | 582 | 348 | 860 | 1.32e-126 | 394 |
| MS.gene82966.t1 | AT3G14810 | 42.970 | 505 | 253 | 8 | 110 | 582 | 358 | 859 | 2.09e-125 | 390 |
| MS.gene82966.t1 | AT2G17010 | 42.292 | 506 | 255 | 10 | 110 | 582 | 389 | 890 | 3.97e-121 | 380 |
| MS.gene82966.t1 | AT1G78610 | 42.012 | 507 | 255 | 8 | 110 | 582 | 331 | 832 | 1.71e-120 | 377 |
| MS.gene82966.t1 | AT2G17000 | 42.688 | 506 | 253 | 10 | 110 | 582 | 322 | 823 | 3.56e-118 | 370 |
| MS.gene82966.t1 | AT2G17000 | 42.688 | 506 | 253 | 10 | 110 | 582 | 322 | 823 | 4.98e-118 | 370 |
| MS.gene82966.t1 | AT3G14810 | 39.883 | 514 | 259 | 11 | 113 | 582 | 317 | 824 | 7.30e-107 | 341 |
| MS.gene82966.t1 | AT2G17010 | 37.786 | 262 | 126 | 10 | 110 | 338 | 389 | 646 | 1.76e-35 | 142 |
Find 111 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGATCTTCCTTTAGAAAT+TGG | 0.137575 | 8.1:+33585785 | MS.gene82966:CDS |
| TCTAGGACTATTGATGATTT+TGG | 0.142127 | 8.1:+33582949 | MS.gene82966:CDS |
| TCAATTGCATCACTCATTTC+TGG | 0.158373 | 8.1:-33587412 | None:intergenic |
| ACACCTTGTTGAGGAATTTA+TGG | 0.163154 | 8.1:-33582552 | None:intergenic |
| GTTAATTATGTTAGGTCTTC+AGG | 0.186545 | 8.1:+33582913 | MS.gene82966:CDS |
| TTTGAAGGTGCTCTTGAAAC+TGG | 0.257587 | 8.1:+33585754 | MS.gene82966:CDS |
| TGGTTCGAGGAGGATTGATA+TGG | 0.264557 | 8.1:+33582831 | MS.gene82966:CDS |
| TCTGGTCCACCGGGCGAAAA+TGG | 0.273685 | 8.1:+33582724 | MS.gene82966:CDS |
| ACCATAATTCTGGTGGTTAA+TGG | 0.274430 | 8.1:-33588864 | None:intergenic |
| CAGGTTTGTCAACAATTTCT+AGG | 0.287188 | 8.1:+33582932 | MS.gene82966:CDS |
| CATCCTCTGATTCTTCTTCC+TGG | 0.298721 | 8.1:-33582138 | None:intergenic |
| CATCTCACTCAAATGAATAT+TGG | 0.302722 | 8.1:+33588980 | MS.gene82966:CDS |
| ACCATTAACCACCAGAATTA+TGG | 0.311068 | 8.1:+33588863 | MS.gene82966:CDS |
| GTATTCAAGGATTCGAGATT+TGG | 0.323814 | 8.1:-33582011 | None:intergenic |
| CAACCCTAAACCTTCTTCTA+TGG | 0.328740 | 8.1:+33581895 | MS.gene82966:CDS |
| TGCCTCCGGGTGTGGGGAGG+TGG | 0.332523 | 8.1:+33582791 | MS.gene82966:CDS |
| GTGTCCATAGAAGAAGGTTT+AGG | 0.335239 | 8.1:-33581899 | None:intergenic |
| TACTTCTCTCACCATAATTC+TGG | 0.340683 | 8.1:-33588874 | None:intergenic |
| TACTTACTTGGCACCAGGTT+TGG | 0.348312 | 8.1:-33583043 | None:intergenic |
| CAGATCTTCCTTTAGAAATT+GGG | 0.353026 | 8.1:+33585786 | MS.gene82966:CDS |
| AAGTTGTTGCACTGCTGTTT+TGG | 0.353295 | 8.1:-33586043 | None:intergenic |
| TGGAAGAAGATGAAGAGATT+TGG | 0.355511 | 8.1:+33582811 | MS.gene82966:CDS |
| AAAAGCTTGTTAATTATGTT+AGG | 0.357821 | 8.1:+33582905 | MS.gene82966:CDS |
| TGGAGAAGCTTAGACGGTTA+AGG | 0.358941 | 8.1:-33581985 | None:intergenic |
| TGTCCATAGAAGAAGGTTTA+GGG | 0.361650 | 8.1:-33581898 | None:intergenic |
| GAAGTTGAAGAAACTTAGTA+TGG | 0.366233 | 8.1:+33582855 | MS.gene82966:CDS |
| TTTGCCATCACACTATGCTT+TGG | 0.367635 | 8.1:-33588791 | None:intergenic |
| AATAATAATTGTGACCCTTT+TGG | 0.369704 | 8.1:+33586098 | MS.gene82966:CDS |
| TTTGCTCTAGAGGGATGTTT+AGG | 0.372443 | 8.1:-33581959 | None:intergenic |
| TTGGTTAGTGAAAATTGTTT+TGG | 0.374026 | 8.1:+33582612 | MS.gene82966:CDS |
| GGAGAAGCTTAGACGGTTAA+GGG | 0.374557 | 8.1:-33581984 | None:intergenic |
| AAGGTTTAGGGTTGTGTTGT+TGG | 0.374758 | 8.1:-33581886 | None:intergenic |
| ACCTTCAAAGAGTGGAAATA+TGG | 0.382658 | 8.1:-33585740 | None:intergenic |
| TATTCAAGGATTCGAGATTT+GGG | 0.392191 | 8.1:-33582010 | None:intergenic |
| TGCTTTGGATTCCAATACTT+TGG | 0.400245 | 8.1:-33588776 | None:intergenic |
| TGGAGAGTAGTGGTTCTGTT+TGG | 0.408314 | 8.1:+33582875 | MS.gene82966:CDS |
| AGATGAAGAGATTTGGTTCG+AGG | 0.412885 | 8.1:+33582818 | MS.gene82966:CDS |
| TATGTGTTGGATGCATTGTC+TGG | 0.417172 | 8.1:+33582706 | MS.gene82966:CDS |
| AGGCTTCTCTTGATGAGGTT+CGG | 0.417205 | 8.1:-33581939 | None:intergenic |
| ACCATATTTCCACTCTTTGA+AGG | 0.420079 | 8.1:+33585739 | MS.gene82966:CDS |
| TTAGTGAGCAAGATTGCGTT+AGG | 0.423646 | 8.1:-33587364 | None:intergenic |
| GGATGCATTGTCTGGTCCAC+CGG | 0.427596 | 8.1:+33582714 | MS.gene82966:CDS |
| ACTCAAATGAATATTGGAAA+TGG | 0.435631 | 8.1:+33588986 | MS.gene82966:CDS |
| AAAGACGATGCCTCCGGGTG+TGG | 0.438076 | 8.1:+33582783 | MS.gene82966:CDS |
| GAAGAGAATAACTTGAGGTT+CGG | 0.441585 | 8.1:-33581858 | None:intergenic |
| AAGGATTCGAGATTTGGGTT+TGG | 0.441587 | 8.1:-33582005 | None:intergenic |
| CAACGGTCTCCAATATCAAA+AGG | 0.442822 | 8.1:-33586246 | None:intergenic |
| TTCAACAATGTTGCCAAACC+TGG | 0.445464 | 8.1:+33583030 | MS.gene82966:CDS |
| GAGGTGGATGGTTGTATTCA+AGG | 0.446525 | 8.1:-33582024 | None:intergenic |
| ATCGAAAGAGCATCCTTTGA+AGG | 0.453490 | 8.1:+33582756 | MS.gene82966:CDS |
| CAATATTCATTTGAGTGAGA+TGG | 0.454096 | 8.1:-33588979 | None:intergenic |
| GAGTGAGATGGATTTCTTGA+GGG | 0.454915 | 8.1:-33588967 | None:intergenic |
| GGATTTCTTGAGGGAGAAGA+TGG | 0.463123 | 8.1:-33588958 | None:intergenic |
| TAGGGTTGTGTTGTTGGTCA+AGG | 0.463940 | 8.1:-33581880 | None:intergenic |
| AGGCACTTGCAAAACTGTCT+TGG | 0.470534 | 8.1:+33586194 | MS.gene82966:CDS |
| GGGTTCAAAGACGATGCCTC+CGG | 0.475509 | 8.1:+33582777 | MS.gene82966:CDS |
| AGTGCAATGATAGTCTCTAT+AGG | 0.476490 | 8.1:-33587457 | None:intergenic |
| AGAGTGAAATTAGTAGTGAA+TGG | 0.480411 | 8.1:+33582980 | MS.gene82966:CDS |
| TAGGATATCAGAACTCCTCT+TGG | 0.481998 | 8.1:+33588898 | MS.gene82966:CDS |
| TTGAGAGCAAACCAAAGTAT+TGG | 0.485327 | 8.1:+33588765 | MS.gene82966:CDS |
| TAGCAACTTCCATCACCAAA+AGG | 0.489311 | 8.1:-33586113 | None:intergenic |
| ACTTACCACCCAATTTCTAA+AGG | 0.492720 | 8.1:-33585794 | None:intergenic |
| CTTGATGAGGTTCGGTGTTG+TGG | 0.494184 | 8.1:-33581931 | None:intergenic |
| AAGACGATGCCTCCGGGTGT+GGG | 0.502898 | 8.1:+33582784 | MS.gene82966:CDS |
| GTTTCAGTGTCCATAGAAGA+AGG | 0.503056 | 8.1:-33581905 | None:intergenic |
| TTCTTCCACCTCCCCACACC+CGG | 0.507949 | 8.1:-33582796 | None:intergenic |
| TATAGGAGTTGTAACATCAA+TGG | 0.509480 | 8.1:-33587440 | None:intergenic |
| GTGTCTTCTTCTTCAGAGGT+AGG | 0.512031 | 8.1:-33582058 | None:intergenic |
| CGATGCCTCCGGGTGTGGGG+AGG | 0.515050 | 8.1:+33582788 | MS.gene82966:CDS |
| TTGTGTCATTGACGGTGTCC+AGG | 0.516753 | 8.1:+33586266 | MS.gene82966:CDS |
| CGATAGCGACTATGAATACC+AGG | 0.518235 | 8.1:+33582120 | MS.gene82966:CDS |
| TGTTTAGGCTTCTCTTGATG+AGG | 0.519840 | 8.1:-33581944 | None:intergenic |
| GGAAGAAGAATCAGAGGATG+AGG | 0.528727 | 8.1:+33582141 | MS.gene82966:CDS |
| GGCAAAACAAATAGATCAAG+TGG | 0.534255 | 8.1:+33588808 | MS.gene82966:CDS |
| TTGAAGGTGCTCTTGAAACT+GGG | 0.539718 | 8.1:+33585755 | MS.gene82966:CDS |
| AGACGATGCCTCCGGGTGTG+GGG | 0.542932 | 8.1:+33582785 | MS.gene82966:CDS |
| GTAGGTAGTTTGTTGCGAGG+TGG | 0.543035 | 8.1:-33582040 | None:intergenic |
| TGAAGAGATTTGGTTCGAGG+AGG | 0.543710 | 8.1:+33582821 | MS.gene82966:CDS |
| GGAGACCGTTGTGTCATTGA+CGG | 0.544383 | 8.1:+33586258 | MS.gene82966:CDS |
| GATGCATTGTCTGGTCCACC+GGG | 0.546646 | 8.1:+33582715 | MS.gene82966:CDS |
| TTAATAGGAGTTGCTTTCCA+AGG | 0.546937 | 8.1:+33586174 | MS.gene82966:CDS |
| ATCGTCTTTGAACCCTTCAA+AGG | 0.553009 | 8.1:-33582769 | None:intergenic |
| GTAGTTTGTTGCGAGGTGGA+TGG | 0.556267 | 8.1:-33582036 | None:intergenic |
| TTGAGTGTCTTCTTCTTCAG+AGG | 0.558346 | 8.1:-33582062 | None:intergenic |
| GGTTCAAAGACGATGCCTCC+GGG | 0.560841 | 8.1:+33582778 | MS.gene82966:CDS |
| AACGGTCTCCAATATCAAAA+GGG | 0.561853 | 8.1:-33586245 | None:intergenic |
| ATCTTCCTTTAGAAATTGGG+TGG | 0.572304 | 8.1:+33585789 | MS.gene82966:CDS |
| TAATTATTTACAGATGATTG+TGG | 0.572993 | 8.1:+33587282 | MS.gene82966:intron |
| TCGAAAGAGCATCCTTTGAA+GGG | 0.574957 | 8.1:+33582757 | MS.gene82966:CDS |
| AAATATACTTACTTGGCACC+AGG | 0.575137 | 8.1:-33583048 | None:intergenic |
| AGCAACTTCCATCACCAAAA+GGG | 0.577274 | 8.1:-33586112 | None:intergenic |
| TCAAGAGCACCTTCAAAGAG+TGG | 0.582307 | 8.1:-33585748 | None:intergenic |
| TCAGAAGAGATCTTGTATGT+AGG | 0.583061 | 8.1:-33582088 | None:intergenic |
| TGGGTTTGGAGAAGCTTAGA+CGG | 0.588604 | 8.1:-33581991 | None:intergenic |
| TGAGTGAGATGGATTTCTTG+AGG | 0.589719 | 8.1:-33588968 | None:intergenic |
| TTCTCTCACCATAATTCTGG+TGG | 0.590623 | 8.1:-33588871 | None:intergenic |
| CATGATGAAGACAAAGATGA+TGG | 0.594026 | 8.1:-33586220 | None:intergenic |
| GAATCCAAAGCATAGTGTGA+TGG | 0.621159 | 8.1:+33588787 | MS.gene82966:CDS |
| ATACCAGGAAGAAGAATCAG+AGG | 0.622604 | 8.1:+33582135 | MS.gene82966:CDS |
| TAGTGAGCAAGATTGCGTTA+GGG | 0.638961 | 8.1:-33587363 | None:intergenic |
| GAGGTAGGTAGTTTGTTGCG+AGG | 0.642897 | 8.1:-33582043 | None:intergenic |
| ATCAGAGGATGAGGACGACG+AGG | 0.643308 | 8.1:+33582150 | MS.gene82966:CDS |
| TCCACCGGGCGAAAATGGCG+AGG | 0.648284 | 8.1:+33582729 | MS.gene82966:CDS |
| TTCCACCTCCCCACACCCGG+AGG | 0.648589 | 8.1:-33582793 | None:intergenic |
| GTGCTTGAAACACCTTGTTG+AGG | 0.657441 | 8.1:-33582561 | None:intergenic |
| ATTATGGTGAGAGAAGTACT+AGG | 0.657963 | 8.1:+33588879 | MS.gene82966:CDS |
| AAACTTAGTATGGAGAGTAG+TGG | 0.659135 | 8.1:+33582865 | MS.gene82966:CDS |
| TCAGAGGATGAGGACGACGA+GGG | 0.689343 | 8.1:+33582151 | MS.gene82966:CDS |
| TCAAGGAAGAGAATAACTTG+AGG | 0.689798 | 8.1:-33581863 | None:intergenic |
| AAACCATAAATTCCTCAACA+AGG | 0.693061 | 8.1:+33582549 | MS.gene82966:CDS |
| GGACACCGTCAATGACACAA+CGG | 0.732774 | 8.1:-33586263 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 33581845 | 33589030 | 33581845 | ID=MS.gene82966 |
| chr8.1 | mRNA | 33581845 | 33589030 | 33581845 | ID=MS.gene82966.t1;Parent=MS.gene82966 |
| chr8.1 | exon | 33581845 | 33582172 | 33581845 | ID=MS.gene82966.t1.exon1;Parent=MS.gene82966.t1 |
| chr8.1 | CDS | 33581845 | 33582172 | 33581845 | ID=cds.MS.gene82966.t1;Parent=MS.gene82966.t1 |
| chr8.1 | exon | 33582497 | 33583058 | 33582497 | ID=MS.gene82966.t1.exon2;Parent=MS.gene82966.t1 |
| chr8.1 | CDS | 33582497 | 33583058 | 33582497 | ID=cds.MS.gene82966.t1;Parent=MS.gene82966.t1 |
| chr8.1 | exon | 33585687 | 33585810 | 33585687 | ID=MS.gene82966.t1.exon3;Parent=MS.gene82966.t1 |
| chr8.1 | CDS | 33585687 | 33585810 | 33585687 | ID=cds.MS.gene82966.t1;Parent=MS.gene82966.t1 |
| chr8.1 | exon | 33585994 | 33586287 | 33585994 | ID=MS.gene82966.t1.exon4;Parent=MS.gene82966.t1 |
| chr8.1 | CDS | 33585994 | 33586287 | 33585994 | ID=cds.MS.gene82966.t1;Parent=MS.gene82966.t1 |
| chr8.1 | exon | 33587295 | 33587497 | 33587295 | ID=MS.gene82966.t1.exon5;Parent=MS.gene82966.t1 |
| chr8.1 | CDS | 33587295 | 33587497 | 33587295 | ID=cds.MS.gene82966.t1;Parent=MS.gene82966.t1 |
| chr8.1 | exon | 33588760 | 33589030 | 33588760 | ID=MS.gene82966.t1.exon6;Parent=MS.gene82966.t1 |
| chr8.1 | CDS | 33588760 | 33589030 | 33588760 | ID=cds.MS.gene82966.t1;Parent=MS.gene82966.t1 |
| Gene Sequence |
| Protein sequence |
