Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83104.t1 | XP_003589658.1 | 100 | 141 | 0 | 0 | 1 | 141 | 1 | 141 | 1.90E-73 | 285 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83104.t1 | Q56XJ7 | 35.1 | 114 | 71 | 3 | 9 | 121 | 113 | 224 | 1.7e-06 | 53.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83104.t1 | Q2HTL2 | 100.0 | 141 | 0 | 0 | 1 | 141 | 1 | 141 | 1.3e-73 | 285.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04920 | MS.gene83104 | 0.806619 | 7.53E-50 | -1.69E-46 |
| MS.gene049499 | MS.gene83104 | 0.801348 | 9.40E-49 | -1.69E-46 |
| MS.gene057893 | MS.gene83104 | -0.814836 | 1.26E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83104.t1 | MTR_1g031680 | 100.000 | 141 | 0 | 0 | 1 | 141 | 1 | 141 | 7.74e-103 | 290 |
| MS.gene83104.t1 | MTR_3g065950 | 53.030 | 132 | 50 | 5 | 7 | 133 | 7 | 131 | 6.71e-39 | 128 |
| MS.gene83104.t1 | MTR_3g065960 | 50.355 | 141 | 58 | 6 | 7 | 141 | 7 | 141 | 9.40e-37 | 123 |
| MS.gene83104.t1 | MTR_5g078750 | 53.097 | 113 | 47 | 2 | 18 | 130 | 41 | 147 | 1.50e-34 | 118 |
| MS.gene83104.t1 | MTR_2g084805 | 32.710 | 107 | 69 | 1 | 18 | 121 | 60 | 166 | 1.84e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83104.t1 | AT1G70780 | 58.042 | 143 | 54 | 4 | 1 | 141 | 2 | 140 | 3.86e-49 | 154 |
| MS.gene83104.t1 | AT1G23150 | 53.378 | 148 | 55 | 7 | 1 | 141 | 1 | 141 | 5.13e-40 | 131 |
| MS.gene83104.t1 | AT5G37730 | 48.980 | 98 | 45 | 2 | 8 | 101 | 3 | 99 | 9.14e-26 | 96.3 |
Find 38 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAATGGGAAGAAACTTTA+AGG | 0.182708 | 1.2:-16549033 | None:intergenic |
| TTTCTCTATTGTCCTCATCT+TGG | 0.304780 | 1.2:+16548146 | MS.gene83104:CDS |
| TCTCAATTTGATCGATTAAT+GGG | 0.318608 | 1.2:-16549047 | None:intergenic |
| GTGCAGCGGCTTTGAGTCCT+TGG | 0.325626 | 1.2:+16548861 | MS.gene83104:intron |
| ATCAATGTGCTTGGAAGTGC+TGG | 0.373268 | 1.2:+16548005 | MS.gene83104:CDS |
| GAAGGGAGATTACCTGTCTT+GGG | 0.392071 | 1.2:+16548104 | MS.gene83104:CDS |
| GGGAAGAAACTTTAAGGTTG+AGG | 0.439231 | 1.2:-16549027 | None:intergenic |
| TTGAGTCCTTGGGATAAAAT+CGG | 0.442562 | 1.2:+16548872 | MS.gene83104:CDS |
| TGTCCTCATCTTGGATCTGA+TGG | 0.450957 | 1.2:+16548155 | MS.gene83104:CDS |
| TGAAGGGAGATTACCTGTCT+TGG | 0.463172 | 1.2:+16548103 | MS.gene83104:CDS |
| AATGAGGCAGCTGCTGAAGA+TGG | 0.479908 | 1.2:+16548947 | MS.gene83104:CDS |
| TGCAGCGGCTTTGAGTCCTT+GGG | 0.495073 | 1.2:+16548862 | MS.gene83104:intron |
| GCCTCATTTGTAGCCGCCTG+TGG | 0.500954 | 1.2:-16548932 | None:intergenic |
| TCACTTCCACGCAGGGGAAG+TGG | 0.505784 | 1.2:+16548983 | MS.gene83104:CDS |
| CACGCAGGGGAAGTGGTAGT+TGG | 0.508737 | 1.2:+16548990 | MS.gene83104:CDS |
| GATTCGATTTGTTGTGAATG+AGG | 0.514352 | 1.2:+16548031 | MS.gene83104:CDS |
| TTGATTAGCATCAATGTGCT+TGG | 0.515963 | 1.2:+16547996 | MS.gene83104:CDS |
| GAAGTGGTAGTTGGAAGTCT+TGG | 0.532728 | 1.2:+16548999 | MS.gene83104:CDS |
| CTGTGATCATTCCCCAAGAC+AGG | 0.539887 | 1.2:-16548116 | None:intergenic |
| TCATTCACAACAAATCGAAT+CGG | 0.546073 | 1.2:-16548029 | None:intergenic |
| CGTGCTATGCAAGAAGCCAC+AGG | 0.559653 | 1.2:+16548916 | MS.gene83104:CDS |
| GCCACAGGCGGCTACAAATG+AGG | 0.559935 | 1.2:+16548931 | MS.gene83104:CDS |
| AACTTCATCACTTCCACGCA+GGG | 0.568118 | 1.2:+16548976 | MS.gene83104:CDS |
| GCAGCTGCTGAAGATGGAAG+TGG | 0.570710 | 1.2:+16548953 | MS.gene83104:CDS |
| AAATCGGATCACATGGAGCT+CGG | 0.572393 | 1.2:+16548888 | MS.gene83104:CDS |
| CTGAAGTCTTATGCACGTGA+AGG | 0.588490 | 1.2:+16548086 | MS.gene83104:CDS |
| TGAAGTCTTATGCACGTGAA+GGG | 0.597124 | 1.2:+16548087 | MS.gene83104:CDS |
| TGGGATAAAATCGGATCACA+TGG | 0.616833 | 1.2:+16548881 | MS.gene83104:CDS |
| GAACTTCATCACTTCCACGC+AGG | 0.619870 | 1.2:+16548975 | MS.gene83104:CDS |
| AAGGGAGATTACCTGTCTTG+GGG | 0.634630 | 1.2:+16548105 | MS.gene83104:CDS |
| ATGCGAATAATGCGAAGAGG+AGG | 0.644335 | 1.2:+16547970 | MS.gene83104:CDS |
| CAACTACCACTTCCCCTGCG+TGG | 0.647554 | 1.2:-16548989 | None:intergenic |
| ATACCATCAGATCCAAGATG+AGG | 0.678101 | 1.2:-16548158 | None:intergenic |
| TCAATGTGCTTGGAAGTGCT+GGG | 0.680964 | 1.2:+16548006 | MS.gene83104:CDS |
| AGAATGCGAATAATGCGAAG+AGG | 0.692570 | 1.2:+16547967 | MS.gene83104:CDS |
| GCTATGCAAGAAGCCACAGG+CGG | 0.698284 | 1.2:+16548919 | MS.gene83104:CDS |
| ACTTCATCACTTCCACGCAG+GGG | 0.703334 | 1.2:+16548977 | MS.gene83104:CDS |
| GAATGAGGAAGAGCTTGTTG+AGG | 0.755960 | 1.2:+16548046 | MS.gene83104:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACAATTAAACATAAGTTTA+TGG | + | chr1.2:16548746-16548765 | MS.gene83104:intron | 15.0% |
| !! | TCTATCTTCATAAACAATAT+TGG | - | chr1.2:16548263-16548282 | None:intergenic | 20.0% |
| ! | AATCACATATTTCACAGTTT+AGG | - | chr1.2:16548550-16548569 | None:intergenic | 25.0% |
| ! | TTGAAAAAACTTGGAAAACA+TGG | + | chr1.2:16548499-16548518 | MS.gene83104:intron | 25.0% |
| !! | TAGAGATGAATGAAAAGTAT+AGG | + | chr1.2:16548283-16548302 | MS.gene83104:intron | 25.0% |
| !! | TCTTGTTTGTTGAAAAAACT+TGG | + | chr1.2:16548490-16548509 | MS.gene83104:intron | 25.0% |
| !!! | TCTTTTGACTTATTAGATTG+TGG | + | chr1.2:16548715-16548734 | MS.gene83104:intron | 25.0% |
| !!! | TTTTTTCAACAAACAAGAGT+CGG | - | chr1.2:16548488-16548507 | None:intergenic | 25.0% |
| AACAAGTATGATGCAAGTAA+TGG | - | chr1.2:16548780-16548799 | None:intergenic | 30.0% | |
| AATGACTTACCTTTGTAGTT+TGG | + | chr1.2:16548334-16548353 | MS.gene83104:intron | 30.0% | |
| ATGTCAATGCAAAATTAGCT+CGG | - | chr1.2:16548681-16548700 | None:intergenic | 30.0% | |
| CATTATAAGCAAGTAGTTGA+TGG | + | chr1.2:16548454-16548473 | MS.gene83104:intron | 30.0% | |
| CTTTATCAGAAATGTTGAAG+TGG | + | chr1.2:16548418-16548437 | MS.gene83104:intron | 30.0% | |
| TCATTCACAACAAATCGAAT+CGG | - | chr1.2:16548032-16548051 | None:intergenic | 30.0% | |
| !! | TTTTTCAACAAACAAGAGTC+GGG | - | chr1.2:16548487-16548506 | None:intergenic | 30.0% |
| ACCATAAGTCCAAACTACAA+AGG | - | chr1.2:16548346-16548365 | None:intergenic | 35.0% | |
| ACCTTTGTAGTTTGGACTTA+TGG | + | chr1.2:16548342-16548361 | MS.gene83104:intron | 35.0% | |
| TTGAGTCCTTGGGATAAAAT+CGG | + | chr1.2:16548872-16548891 | MS.gene83104:CDS | 35.0% | |
| TTGATTAGCATCAATGTGCT+TGG | + | chr1.2:16547996-16548015 | MS.gene83104:CDS | 35.0% | |
| TTTCTCTATTGTCCTCATCT+TGG | + | chr1.2:16548146-16548165 | MS.gene83104:CDS | 35.0% | |
| ! | TTTTCAACAAACAAGAGTCG+GGG | - | chr1.2:16548486-16548505 | None:intergenic | 35.0% |
| !! | GATTCGATTTGTTGTGAATG+AGG | + | chr1.2:16548031-16548050 | MS.gene83104:CDS | 35.0% |
| AGAATGCGAATAATGCGAAG+AGG | + | chr1.2:16547967-16547986 | MS.gene83104:CDS | 40.0% | |
| ATACCATCAGATCCAAGATG+AGG | - | chr1.2:16548161-16548180 | None:intergenic | 40.0% | |
| CAGAAATGTTGAAGTGGTGA+GGG | + | chr1.2:16548424-16548443 | MS.gene83104:intron | 40.0% | |
| TCAGAAATGTTGAAGTGGTG+AGG | + | chr1.2:16548423-16548442 | MS.gene83104:intron | 40.0% | |
| TGGGATAAAATCGGATCACA+TGG | + | chr1.2:16548881-16548900 | MS.gene83104:CDS | 40.0% | |
| TTCACAGTTTAGGAATCTGC+TGG | - | chr1.2:16548540-16548559 | None:intergenic | 40.0% | |
| ! | TGAAGTCTTATGCACGTGAA+GGG | + | chr1.2:16548087-16548106 | MS.gene83104:CDS | 40.0% |
| ! | TGTGATCCGATTTTATCCCA+AGG | - | chr1.2:16548881-16548900 | None:intergenic | 40.0% |
| !! | GGGAAGAAACTTTAAGGTTG+AGG | - | chr1.2:16549030-16549049 | None:intergenic | 40.0% |
| AAATCGGATCACATGGAGCT+CGG | + | chr1.2:16548888-16548907 | MS.gene83104:CDS | 45.0% | |
| AACTTCATCACTTCCACGCA+GGG | + | chr1.2:16548976-16548995 | MS.gene83104:CDS | 45.0% | |
| AAGGGAGATTACCTGTCTTG+GGG | + | chr1.2:16548105-16548124 | MS.gene83104:CDS | 45.0% | |
| AATCATGCTAACCCTCTCTC+TGG | + | chr1.2:16548824-16548843 | MS.gene83104:intron | 45.0% | |
| ATGCGAATAATGCGAAGAGG+AGG | + | chr1.2:16547970-16547989 | MS.gene83104:CDS | 45.0% | |
| CAAACCACAATCCAGAGAGA+GGG | - | chr1.2:16548838-16548857 | None:intergenic | 45.0% | |
| CTCTCTGGATTGTGGTTTGA+TGG | + | chr1.2:16548839-16548858 | MS.gene83104:intron | 45.0% | |
| GAAGGGAGATTACCTGTCTT+GGG | + | chr1.2:16548104-16548123 | MS.gene83104:CDS | 45.0% | |
| GAAGTGGTAGTTGGAAGTCT+TGG | + | chr1.2:16548999-16549018 | MS.gene83104:CDS | 45.0% | |
| TCAAACCACAATCCAGAGAG+AGG | - | chr1.2:16548839-16548858 | None:intergenic | 45.0% | |
| TGAAGGGAGATTACCTGTCT+TGG | + | chr1.2:16548103-16548122 | MS.gene83104:CDS | 45.0% | |
| TGTCCTCATCTTGGATCTGA+TGG | + | chr1.2:16548155-16548174 | MS.gene83104:CDS | 45.0% | |
| TGTTGAAGTGGTGAGGGATT+CGG | + | chr1.2:16548430-16548449 | MS.gene83104:intron | 45.0% | |
| ! | ATCAATGTGCTTGGAAGTGC+TGG | + | chr1.2:16548005-16548024 | MS.gene83104:CDS | 45.0% |
| ! | CTGAAGTCTTATGCACGTGA+AGG | + | chr1.2:16548086-16548105 | MS.gene83104:CDS | 45.0% |
| !! | ATTGTGGTTTGATGGTGCAG+CGG | + | chr1.2:16548847-16548866 | MS.gene83104:intron | 45.0% |
| !! | GAATGAGGAAGAGCTTGTTG+AGG | + | chr1.2:16548046-16548065 | MS.gene83104:CDS | 45.0% |
| !! | TCAATGTGCTTGGAAGTGCT+GGG | + | chr1.2:16548006-16548025 | MS.gene83104:CDS | 45.0% |
| AATGAGGCAGCTGCTGAAGA+TGG | + | chr1.2:16548947-16548966 | MS.gene83104:CDS | 50.0% | |
| ACTTCATCACTTCCACGCAG+GGG | + | chr1.2:16548977-16548996 | MS.gene83104:CDS | 50.0% | |
| CTAACCCTCTCTCTGGATTG+TGG | + | chr1.2:16548831-16548850 | MS.gene83104:intron | 50.0% | |
| CTGTGATCATTCCCCAAGAC+AGG | - | chr1.2:16548119-16548138 | None:intergenic | 50.0% | |
| GAACTTCATCACTTCCACGC+AGG | + | chr1.2:16548975-16548994 | MS.gene83104:CDS | 50.0% | |
| GTTGAAGTGGTGAGGGATTC+GGG | + | chr1.2:16548431-16548450 | MS.gene83104:intron | 50.0% | |
| CGTGCTATGCAAGAAGCCAC+AGG | + | chr1.2:16548916-16548935 | MS.gene83104:CDS | 55.0% | |
| GCAGCTGCTGAAGATGGAAG+TGG | + | chr1.2:16548953-16548972 | MS.gene83104:CDS | 55.0% | |
| GCTATGCAAGAAGCCACAGG+CGG | + | chr1.2:16548919-16548938 | MS.gene83104:CDS | 55.0% | |
| !! | TGCAGCGGCTTTGAGTCCTT+GGG | + | chr1.2:16548862-16548881 | MS.gene83104:intron | 55.0% |
| CAACTACCACTTCCCCTGCG+TGG | - | chr1.2:16548992-16549011 | None:intergenic | 60.0% | |
| CACGCAGGGGAAGTGGTAGT+TGG | + | chr1.2:16548990-16549009 | MS.gene83104:CDS | 60.0% | |
| GCCACAGGCGGCTACAAATG+AGG | + | chr1.2:16548931-16548950 | MS.gene83104:CDS | 60.0% | |
| GCCTCATTTGTAGCCGCCTG+TGG | - | chr1.2:16548935-16548954 | None:intergenic | 60.0% | |
| TCACTTCCACGCAGGGGAAG+TGG | + | chr1.2:16548983-16549002 | MS.gene83104:CDS | 60.0% | |
| !! | GTGCAGCGGCTTTGAGTCCT+TGG | + | chr1.2:16548861-16548880 | MS.gene83104:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 16547939 | 16549054 | 16547939 | ID=MS.gene83104 |
| chr1.2 | mRNA | 16547939 | 16549054 | 16547939 | ID=MS.gene83104.t1;Parent=MS.gene83104 |
| chr1.2 | exon | 16547939 | 16548176 | 16547939 | ID=MS.gene83104.t1.exon1;Parent=MS.gene83104.t1 |
| chr1.2 | CDS | 16547939 | 16548176 | 16547939 | ID=cds.MS.gene83104.t1;Parent=MS.gene83104.t1 |
| chr1.2 | exon | 16548867 | 16549054 | 16548867 | ID=MS.gene83104.t1.exon2;Parent=MS.gene83104.t1 |
| chr1.2 | CDS | 16548867 | 16549054 | 16548867 | ID=cds.MS.gene83104.t1;Parent=MS.gene83104.t1 |
| Gene Sequence |
| Protein sequence |
