AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene83178

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83178.t1 MTR_4g078160 95.676 185 8 0 1 185 209 393 4.26e-122 350
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene83178.t1 AT1G75340 42.500 120 41 4 74 184 278 378 1.22e-12 65.5
MS.gene83178.t1 AT1G75340 42.500 120 41 4 74 184 298 398 1.24e-12 65.5
MS.gene83178.t1 AT1G75340 42.500 120 41 4 74 184 335 435 1.37e-12 65.5

Find 71 sgRNAs with CRISPR-Local

Find 238 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGCTCCACTACAGTGTTTC+AGG 0.252801 4.2:+31853404 MS.gene83178:CDS
CAACGGGTGGTTGTTGGAAC+TGG 0.317831 4.2:-31853368 None:intergenic
GGCATTTACATTGGCTCCAT+TGG 0.328283 4.2:-31851785 None:intergenic
GGATGATAGTATCTGGTTAA+AGG 0.329999 4.2:+31854148 MS.gene83178:CDS
CTATCTGGAGGAGCTTGTTC+TGG 0.339340 4.2:-31854521 None:intergenic
GGCATTTGCATTGGCTCCAT+TGG 0.343025 4.2:-31851836 None:intergenic
TGCACTGGAGTACTCATTAA+TGG 0.355560 4.2:-31853118 None:intergenic
GTCTACCAAACAAATCTATC+TGG 0.360641 4.2:-31854536 None:intergenic
AGTCTCAAGCTTTAGCCAAC+TGG 0.360859 4.2:+31851894 MS.gene83178:CDS
CCTACACCAGCGGGGTTGAC+AGG 0.380081 4.2:+31853327 MS.gene83178:CDS
GCGGGGTTGACAGGGTTTCC+AGG 0.404696 4.2:+31853336 MS.gene83178:CDS
CTAAAGCTTGAGACTGATAA+AGG 0.416164 4.2:-31851886 None:intergenic
GTATCTGGTTAAAGGAGAAA+TGG 0.421007 4.2:+31854156 MS.gene83178:CDS
CCGAATAGATTTCCTGAGTT+GGG 0.442288 4.2:-31853270 None:intergenic
CTCCTCCAGATAGATTTGTT+TGG 0.444104 4.2:+31854531 MS.gene83178:CDS
TGAACCCAACGGGTGGTTGT+TGG 0.446347 4.2:-31853374 None:intergenic
CTATTCGGCAGTGGAATCTC+AGG 0.451076 4.2:+31853285 MS.gene83178:CDS
CAACCAGCCACTCAAAATAA+TGG 0.452627 4.2:+31851862 MS.gene83178:CDS
GTGACTTACAACTGAACATC+TGG 0.457439 4.2:-31853432 None:intergenic
CATTGTTACCTTTCAAAGCC+AGG 0.459429 4.2:-31851931 None:intergenic
CCCAACTCAGGAAATCTATT+CGG 0.463482 4.2:+31853270 MS.gene83178:CDS
TTGCATTGGCTCCATTGGAT+TGG 0.473295 4.2:-31851831 None:intergenic
AGTTCCAACAACCACCCGTT+GGG 0.480716 4.2:+31853370 MS.gene83178:CDS
AACCCCTTTCCTACACCAGC+GGG 0.481599 4.2:+31853318 MS.gene83178:CDS
TTACATTGGCTCCATTGGAT+TGG 0.483725 4.2:-31851780 None:intergenic
AACCCCGCTGGTGTAGGAAA+GGG 0.485644 4.2:-31853321 None:intergenic
CCACAGACACATTCTCCACT+TGG 0.493241 4.2:-31854127 None:intergenic
GTCTCAAGCTTTAGCCAACT+GGG 0.494261 4.2:+31851895 MS.gene83178:CDS
CCAATGGAACTAGGCTGCAC+TGG 0.496298 4.2:-31853133 None:intergenic
CCTCCACCTCCAATGGAACT+AGG 0.507796 4.2:-31853142 None:intergenic
CCAGTGCAGCCTAGTTCCAT+TGG 0.509632 4.2:+31853133 MS.gene83178:CDS
CACTGAACCCTGGCTTTGAA+AGG 0.511359 4.2:+31851923 MS.gene83178:CDS
GCCGAATAGATTTCCTGAGT+TGG 0.514471 4.2:-31853271 None:intergenic
ATACATACCTCCACCTCCAA+TGG 0.518617 4.2:-31853149 None:intergenic
TGGTTGAAAGGCATTTGCAT+TGG 0.523106 4.2:-31851845 None:intergenic
TTAAAGGAGAAATGGAATCC+TGG 0.531099 4.2:+31854164 MS.gene83178:CDS
GTGCAGCCTAGTTCCATTGG+AGG 0.531680 4.2:+31853136 MS.gene83178:CDS
CCTAGTTCCATTGGAGGTGG+AGG 0.532586 4.2:+31853142 MS.gene83178:CDS
TGTCTGTGGATGATAGTATC+TGG 0.532717 4.2:+31854141 MS.gene83178:CDS
TAGAAGACTATTACCTCTCC+AGG 0.535337 4.2:-31854182 None:intergenic
CAGCCTAGTTCCATTGGAGG+TGG 0.537156 4.2:+31853139 MS.gene83178:CDS
TGGAACTGGGAAGTAGAACC+TGG 0.538756 4.2:-31853354 None:intergenic
CTAGTGTTATTGAACCCAAC+GGG 0.544107 4.2:-31853384 None:intergenic
CAACCCCGCTGGTGTAGGAA+AGG 0.545368 4.2:-31853322 None:intergenic
CCTGTCAACCCCGCTGGTGT+AGG 0.552858 4.2:-31853327 None:intergenic
GGAAACCCTGTCAACCCCGC+TGG 0.553188 4.2:-31853333 None:intergenic
CTACACCAGCGGGGTTGACA+GGG 0.559612 4.2:+31853328 MS.gene83178:CDS
AACGGGTGGTTGTTGGAACT+GGG 0.561218 4.2:-31853367 None:intergenic
GTTCAAAACTTCCAATCCAA+TGG 0.564091 4.2:+31851769 MS.gene83178:CDS
ACTCAAAACTTCCAATCCAA+TGG 0.568860 4.2:+31851820 MS.gene83178:CDS
ATTGTTACCTTTCAAAGCCA+GGG 0.569637 4.2:-31851930 None:intergenic
CAGTTCCAACAACCACCCGT+TGG 0.575425 4.2:+31853369 MS.gene83178:CDS
ACCCCGCTGGTGTAGGAAAG+GGG 0.586779 4.2:-31853320 None:intergenic
CTGGGTGCTTCACTGAACCC+TGG 0.589919 4.2:+31851913 MS.gene83178:CDS
TCAGGAAATCTATTCGGCAG+TGG 0.590143 4.2:+31853276 MS.gene83178:CDS
GTACTCATTAATGGTGCAGA+GGG 0.590273 4.2:-31853109 None:intergenic
CACAGACACATTCTCCACTT+GGG 0.605135 4.2:-31854126 None:intergenic
AGGTTGAAAGGCATTTACAT+TGG 0.610948 4.2:-31851794 None:intergenic
TTGCAGAAATGCGTCCCAAG+TGG 0.622726 4.2:+31854112 MS.gene83178:intron
GCTAGTGTTATTGAACCCAA+CGG 0.631795 4.2:-31853385 None:intergenic
TACCAAACAAATCTATCTGG+AGG 0.634565 4.2:-31854533 None:intergenic
GGTTCAGTGAAGCACCCAGT+TGG 0.640946 4.2:-31851909 None:intergenic
TGCTGCCTGAAACACTGTAG+TGG 0.644762 4.2:-31853409 None:intergenic
GTGTTATTGAACCCAACGGG+TGG 0.644954 4.2:-31853381 None:intergenic
TTCGGCAGTGGAATCTCAGG+TGG 0.654074 4.2:+31853288 MS.gene83178:CDS
AGTACTCATTAATGGTGCAG+AGG 0.660040 4.2:-31853110 None:intergenic
GGAGAAATGGAATCCTGGAG+AGG 0.663151 4.2:+31854169 MS.gene83178:CDS
CAACCCCTTTCCTACACCAG+CGG 0.664582 4.2:+31853317 MS.gene83178:CDS
CCAAGTGGAGAATGTGTCTG+TGG 0.682566 4.2:+31854127 MS.gene83178:CDS
ACCCCTTTCCTACACCAGCG+GGG 0.708861 4.2:+31853319 MS.gene83178:CDS
ACGTTGAGAGAAGACGTCAG+AGG 0.742655 4.2:-31851748 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTTAAAAAAATACACA+CGG - chr4.2:31852752-31852771 None:intergenic 10.0%
!! AAATCATGAGAATATAAAAT+AGG - chr4.2:31853032-31853051 None:intergenic 15.0%
!! ATAATTAAAAAAAATACACG+TGG + chr4.2:31852857-31852876 MS.gene83178:intron 15.0%
!! CAAATTATAACATTTATAAC+TGG - chr4.2:31853795-31853814 None:intergenic 15.0%
!! CAGTTATAAATGTTATAATT+TGG + chr4.2:31853793-31853812 MS.gene83178:intron 15.0%
!!! ATATTTTAAAATTCAAGTGT+TGG + chr4.2:31854425-31854444 MS.gene83178:intron 15.0%
!!! TATTTTAAAATTCAAGTGTT+GGG + chr4.2:31854426-31854445 MS.gene83178:intron 15.0%
!!! TTTGTAGTTTTTTTTCTTAA+GGG + chr4.2:31852957-31852976 MS.gene83178:intron 15.0%
!!! TTTTGTAGTTTTTTTTCTTA+AGG + chr4.2:31852956-31852975 MS.gene83178:intron 15.0%
!!! TTTTGTAGTTTTTTTTCTTA+GGG - chr4.2:31852975-31852956 None:intergenic 15.0%
!! AACAAGATATAAACATTGAA+TGG - chr4.2:31852566-31852585 None:intergenic 20.0%
!! AATCTTTAAACTGTAACATA+TGG - chr4.2:31853864-31853883 None:intergenic 20.0%
!! ACAAGATATAAACATTGAAT+GGG - chr4.2:31852565-31852584 None:intergenic 20.0%
!! ATTTGGATATTTCTTGATTT+AGG - chr4.2:31852009-31852028 None:intergenic 20.0%
!! CTATTAAGCCTATAAAATAA+AGG - chr4.2:31852589-31852608 None:intergenic 20.0%
!!! ATACTTTTTCTTGAATCTTA+TGG + chr4.2:31854079-31854098 MS.gene83178:intron 20.0%
!!! ATCTACTATTTGTTTTTGTT+TGG + chr4.2:31852174-31852193 MS.gene83178:intron 20.0%
!!! ATCTTGTTCCTTTATTTTAT+AGG + chr4.2:31852578-31852597 MS.gene83178:intron 20.0%
!!! ATTTTAAAATTCAAGTGTTG+GGG + chr4.2:31854427-31854446 MS.gene83178:intron 20.0%
!!! GATTAAGAAGATTTTAATGT+TGG + chr4.2:31853880-31853899 MS.gene83178:intron 20.0%
!!! GTGAATTTTGTTTTTTTGAA+TGG + chr4.2:31852406-31852425 MS.gene83178:intron 20.0%
!!! GTTTTGTAGTTTTTTTTCTT+AGG - chr4.2:31852974-31852955 None:intergenic 20.0%
!!! TAATGTTAGTTTTTTGTTGT+GGG + chr4.2:31852451-31852470 MS.gene83178:intron 20.0%
!!! TCTACTATTTGTTTTTGTTT+GGG + chr4.2:31852175-31852194 MS.gene83178:intron 20.0%
!!! TTAATGTTAGTTTTTTGTTG+TGG + chr4.2:31852450-31852469 MS.gene83178:intron 20.0%
!!! TTGTAGTTTTTTTTCTTAAG+GGG + chr4.2:31852958-31852977 MS.gene83178:intron 20.0%
!!! TTTGTAGTTTTTTTTCTTAG+GGG - chr4.2:31852653-31852672 None:intergenic 20.0%
! CATGTATCTTTCTATTATAG+TGG + chr4.2:31852232-31852251 MS.gene83178:intron 25.0%
! CTTTCTATTATAGTGGTTTA+AGG + chr4.2:31852239-31852258 MS.gene83178:intron 25.0%
! GATACATACATACATAGTAT+TGG + chr4.2:31854296-31854315 MS.gene83178:intron 25.0%
! TATGTATGTATGTATCACAA+AGG - chr4.2:31854292-31854311 None:intergenic 25.0%
!! AATGATGGTTTTCTTATGTT+TGG + chr4.2:31852363-31852382 MS.gene83178:intron 25.0%
!! CATAAGAAAACCATCATTTT+AGG - chr4.2:31852361-31852380 None:intergenic 25.0%
!! GAAAGTTGTACAACTATTTT+TGG - chr4.2:31853540-31853559 None:intergenic 25.0%
!! GGATTTTATTAAATTGTCAC+AGG - chr4.2:31853498-31853517 None:intergenic 25.0%
!! TTTCTTCATTTTCAATTGTG+TGG + chr4.2:31851970-31851989 MS.gene83178:intron 25.0%
!!! ATATCCAAATTTTAGCTAAC+AGG + chr4.2:31852019-31852038 MS.gene83178:intron 25.0%
!!! CATCATTTTAGGTATGTAAT+CGG - chr4.2:31852350-31852369 None:intergenic 25.0%
!!! TTGTAGTTTTTTTTCTTAGG+GGG - chr4.2:31852652-31852671 None:intergenic 25.0%
!!! TTTGTTTTTGTTTGGGATTA+TGG + chr4.2:31852182-31852201 MS.gene83178:intron 25.0%
!!! TTTTTTTTTATGTCTGCTCT+TGG + chr4.2:31853052-31853071 MS.gene83178:intron 25.0%
AATTGGGTCATTTACTTTGT+AGG - chr4.2:31853769-31853788 None:intergenic 30.0%
ACATTTATAACTGGTGCAAT+TGG - chr4.2:31853786-31853805 None:intergenic 30.0%
ATGATGTTGTGATAAACTGT+AGG + chr4.2:31853234-31853253 MS.gene83178:intron 30.0%
ATTATGGTGTGATTGTCAAA+AGG + chr4.2:31852198-31852217 MS.gene83178:intron 30.0%
ATTGGGTCATTTACTTTGTA+GGG - chr4.2:31853768-31853787 None:intergenic 30.0%
CATTTATAACTGGTGCAATT+GGG - chr4.2:31853785-31853804 None:intergenic 30.0%
CGATTACATACCTAAAATGA+TGG + chr4.2:31852348-31852367 MS.gene83178:intron 30.0%
CTGTTTGTAACTTTCTTGTT+AGG + chr4.2:31853086-31853105 MS.gene83178:intron 30.0%
GGTTCAATACTAACAAAAGT+TGG - chr4.2:31853971-31853990 None:intergenic 30.0%
GGTTGACTATTGAATGAAAA+TGG - chr4.2:31853519-31853538 None:intergenic 30.0%
TACAGTTTATCACAACATCA+TGG - chr4.2:31853235-31853254 None:intergenic 30.0%
TAGATAGCAGTTGTAGAAAA+AGG - chr4.2:31852159-31852178 None:intergenic 30.0%
TATTGGATAGAATCTGCAAT+AGG + chr4.2:31854313-31854332 MS.gene83178:intron 30.0%
TCAATACTAACAAAAGTTGG+AGG - chr4.2:31853968-31853987 None:intergenic 30.0%
TTATGGTGTGATTGTCAAAA+GGG + chr4.2:31852199-31852218 MS.gene83178:intron 30.0%
TTTGCCTGTTAGCTAAAATT+TGG - chr4.2:31852026-31852045 None:intergenic 30.0%
! TCACAAAGGAAATTTTGTCA+TGG - chr4.2:31854278-31854297 None:intergenic 30.0%
! TTGTTAGTATTGAACCTACT+TGG + chr4.2:31853975-31853994 MS.gene83178:intron 30.0%
!! GAAGAAAATGTTCTAAAGCA+TGG - chr4.2:31851957-31851976 None:intergenic 30.0%
!! TTCTGCCAAATTTTAATGCT+CGG - chr4.2:31854381-31854400 None:intergenic 30.0%
!!! TAAAGGACCATTATTTTGAG+TGG - chr4.2:31851872-31851891 None:intergenic 30.0%
AAAGTAGGTCACAAACAATC+AGG - chr4.2:31852102-31852121 None:intergenic 35.0%
ACTCAAAACTTCCAATCCAA+TGG + chr4.2:31851820-31851839 MS.gene83178:CDS 35.0%
ATCGCAACTTCTTGAAACTT+AGG + chr4.2:31852986-31853005 MS.gene83178:intron 35.0%
ATCGCAACTTCTTGAAACTT+AGG - chr4.2:31853005-31852986 None:intergenic 35.0%
ATCGTATAGAGATATTGACG+AGG + chr4.2:31852076-31852095 MS.gene83178:intron 35.0%
ATTGTTACCTTTCAAAGCCA+GGG - chr4.2:31851933-31851952 None:intergenic 35.0%
CAAAGAGCTTAACTGCTATA+CGG + chr4.2:31852047-31852066 MS.gene83178:intron 35.0%
CAACTATCTGAAAATGCTCA+AGG - chr4.2:31853622-31853641 None:intergenic 35.0%
CTAAAGCTTGAGACTGATAA+AGG - chr4.2:31851889-31851908 None:intergenic 35.0%
GAAAGTTAGTGCAGTAACAA+AGG - chr4.2:31854009-31854028 None:intergenic 35.0%
GGATGATAGTATCTGGTTAA+AGG + chr4.2:31854148-31854167 MS.gene83178:CDS 35.0%
GTATCTGGTTAAAGGAGAAA+TGG + chr4.2:31854156-31854175 MS.gene83178:CDS 35.0%
GTTCAAAACTTCCAATCCAA+TGG + chr4.2:31851769-31851788 MS.gene83178:CDS 35.0%
TACCAAACAAATCTATCTGG+AGG - chr4.2:31854536-31854555 None:intergenic 35.0%
TCGCAACTTCTTGAAACTTA+GGG + chr4.2:31852987-31853006 MS.gene83178:intron 35.0%
TCGCAACTTCTTGAAACTTA+GGG - chr4.2:31853006-31852987 None:intergenic 35.0%
TGAAAATGCTCAAGGTCAAT+GGG - chr4.2:31853614-31853633 None:intergenic 35.0%
TGCAACCAACTAAAGAAAGT+AGG - chr4.2:31852117-31852136 None:intergenic 35.0%
TGTGACCTACTTTCTTTAGT+TGG + chr4.2:31852109-31852128 MS.gene83178:intron 35.0%
TGTTACTGCACTAACTTTCT+GGG + chr4.2:31854010-31854029 MS.gene83178:intron 35.0%
TTAAAGGAGAAATGGAATCC+TGG + chr4.2:31854164-31854183 MS.gene83178:CDS 35.0%
TTGGGAATAAGTGTCTATTC+AGG + chr4.2:31854221-31854240 MS.gene83178:intron 35.0%
TTGTTACTGCACTAACTTTC+TGG + chr4.2:31854009-31854028 MS.gene83178:intron 35.0%
! GAATCTGCAATAGGATCTTT+TGG + chr4.2:31854322-31854341 MS.gene83178:intron 35.0%
! GTTTCAAGAAGTTGCGATTT+TGG + chr4.2:31852626-31852645 MS.gene83178:intron 35.0%
! GTTTCAAGAAGTTGCGATTT+TGG - chr4.2:31852645-31852626 None:intergenic 35.0%
! TTTAATGTTGGTAGAGATGC+TGG + chr4.2:31853892-31853911 MS.gene83178:intron 35.0%
!! AGGTTGAAAGGCATTTACAT+TGG - chr4.2:31851797-31851816 None:intergenic 35.0%
!! GTGAATTACATAAGAGTGTC+AGG - chr4.2:31853686-31853705 None:intergenic 35.0%
!!! ATTGGATTGGAAGTTTTGAG+TGG - chr4.2:31851821-31851840 None:intergenic 35.0%
!!! GTTAGTTTTTTGTTGTGGGT+GGG + chr4.2:31852455-31852474 MS.gene83178:intron 35.0%
!!! TGTTAGTTTTTTGTTGTGGG+TGG + chr4.2:31852454-31852473 MS.gene83178:intron 35.0%
!!! TTAGTTTTTTGTTGTGGGTG+GGG + chr4.2:31852456-31852475 MS.gene83178:intron 35.0%
ACTTATTCCCAACCTCAACA+AGG - chr4.2:31854213-31854232 None:intergenic 40.0%
AGAAAAAGGAATCCCTGTAG+AGG - chr4.2:31852145-31852164 None:intergenic 40.0%
AGTACTCATTAATGGTGCAG+AGG - chr4.2:31853113-31853132 None:intergenic 40.0%
ATATAAGGTGGTTGAGGTGT+GGG - chr4.2:31852519-31852538 None:intergenic 40.0%
CAAAGGTGAAGTTTCCAAGT+AGG - chr4.2:31853992-31854011 None:intergenic 40.0%
CAACCAGCCACTCAAAATAA+TGG + chr4.2:31851862-31851881 MS.gene83178:CDS 40.0%
CAAGTTCTGTTGAATGACCT+AGG + chr4.2:31852307-31852326 MS.gene83178:intron 40.0%
CACAACATCATGGTCAAACA+AGG - chr4.2:31853225-31853244 None:intergenic 40.0%
CATTGTTACCTTTCAAAGCC+AGG - chr4.2:31851934-31851953 None:intergenic 40.0%
CCCAACTCAGGAAATCTATT+CGG + chr4.2:31853270-31853289 MS.gene83178:CDS 40.0%
CCGAATAGATTTCCTGAGTT+GGG - chr4.2:31853273-31853292 None:intergenic 40.0%
CGCAACTTCTTGAAACTTAG+GGG + chr4.2:31852988-31853007 MS.gene83178:intron 40.0%
CGCAACTTCTTGAAACTTAG+GGG - chr4.2:31853007-31852988 None:intergenic 40.0%
CGTCTCCGAGCATTAAAATT+TGG + chr4.2:31854373-31854392 MS.gene83178:intron 40.0%
CTGAAAATGCTCAAGGTCAA+TGG - chr4.2:31853615-31853634 None:intergenic 40.0%
GAGAGGTAATAGTCTTCTAC+TGG + chr4.2:31854186-31854205 MS.gene83178:intron 40.0%
GCAACTTCTTGAAACTTAGG+GGG + chr4.2:31852989-31853008 MS.gene83178:intron 40.0%
GCAACTTCTTGAAACTTAGG+GGG - chr4.2:31853008-31852989 None:intergenic 40.0%
GTACTCATTAATGGTGCAGA+GGG - chr4.2:31853112-31853131 None:intergenic 40.0%
GTGACTTACAACTGAACATC+TGG - chr4.2:31853435-31853454 None:intergenic 40.0%
GTTACTGCACTAACTTTCTG+GGG + chr4.2:31854011-31854030 MS.gene83178:intron 40.0%
TAGAAGACTATTACCTCTCC+AGG - chr4.2:31854185-31854204 None:intergenic 40.0%
TGTCTGTGGATGATAGTATC+TGG + chr4.2:31854141-31854160 MS.gene83178:CDS 40.0%
TTATGTCTGCTCTTGGCATA+TGG + chr4.2:31853059-31853078 MS.gene83178:intron 40.0%
! ACCGAGTCAAAATTGGCTTA+GGG + chr4.2:31852897-31852916 MS.gene83178:intron 40.0%
! CTAGTGTTATTGAACCCAAC+GGG - chr4.2:31853387-31853406 None:intergenic 40.0%
! CTCCTCCAGATAGATTTGTT+TGG + chr4.2:31854531-31854550 MS.gene83178:CDS 40.0%
! GCTAGTGTTATTGAACCCAA+CGG - chr4.2:31853388-31853407 None:intergenic 40.0%
! TGCACTGGAGTACTCATTAA+TGG - chr4.2:31853121-31853140 None:intergenic 40.0%
!! ATTTTGAGTGGCTGGTTGAA+AGG - chr4.2:31851860-31851879 None:intergenic 40.0%
!! TGGTTGAAAGGCATTTGCAT+TGG - chr4.2:31851848-31851867 None:intergenic 40.0%
!! TTACATTGGCTCCATTGGAT+TGG - chr4.2:31851783-31851802 None:intergenic 40.0%
!!! AACTTTTTCACACCCAACTC+AGG + chr4.2:31853258-31853277 MS.gene83178:CDS 40.0%
!!! TAGTTTTTTGTTGTGGGTGG+GGG + chr4.2:31852457-31852476 MS.gene83178:intron 40.0%
ACACCAAACTCTCATGACCT+AGG - chr4.2:31852327-31852346 None:intergenic 45.0%
ATACATACCTCCACCTCCAA+TGG - chr4.2:31853152-31853171 None:intergenic 45.0%
CACAGACACATTCTCCACTT+GGG - chr4.2:31854129-31854148 None:intergenic 45.0%
GATATAAGGTGGTTGAGGTG+TGG - chr4.2:31852520-31852539 None:intergenic 45.0%
GCCGAATAGATTTCCTGAGT+TGG - chr4.2:31853274-31853293 None:intergenic 45.0%
GGACGCATTTCTGCAAGAAT+AGG - chr4.2:31854108-31854127 None:intergenic 45.0%
GGTGTGATTGTCAAAAGGGT+GGG + chr4.2:31852203-31852222 MS.gene83178:intron 45.0%
GTGTGATTGTCAAAAGGGTG+GGG + chr4.2:31852204-31852223 MS.gene83178:intron 45.0%
TACTGGTCCTTGTTGAGGTT+GGG + chr4.2:31854203-31854222 MS.gene83178:intron 45.0%
TAGCTCCACTACAGTGTTTC+AGG + chr4.2:31853404-31853423 MS.gene83178:CDS 45.0%
TCAGGAAATCTATTCGGCAG+TGG + chr4.2:31853276-31853295 MS.gene83178:CDS 45.0%
TCTTCTACTGGTCCTTGTTG+AGG + chr4.2:31854198-31854217 MS.gene83178:intron 45.0%
TGACCTAGGTCATGAGAGTT+TGG + chr4.2:31852321-31852340 MS.gene83178:intron 45.0%
TGCCACAGGAGCAAAACTTA+GGG - chr4.2:31852682-31852701 None:intergenic 45.0%
TGGTGTGATTGTCAAAAGGG+TGG + chr4.2:31852202-31852221 MS.gene83178:intron 45.0%
! AGTCTCAAGCTTTAGCCAAC+TGG + chr4.2:31851894-31851913 MS.gene83178:CDS 45.0%
! CCGAGTCAAAATTGGCTTAG+GGG + chr4.2:31852898-31852917 MS.gene83178:intron 45.0%
! GACCGAGTCAAAATTGGCTT+AGG + chr4.2:31852896-31852915 MS.gene83178:intron 45.0%
! GTCTCAAGCTTTAGCCAACT+GGG + chr4.2:31851895-31851914 MS.gene83178:CDS 45.0%
! TGCGTTGACCGAGTCAAAAT+TGG + chr4.2:31852890-31852909 MS.gene83178:intron 45.0%
! TTTTGCTCCTGTGGCAGTTT+TGG + chr4.2:31852686-31852705 MS.gene83178:intron 45.0%
!! GGCATTTACATTGGCTCCAT+TGG - chr4.2:31851788-31851807 None:intergenic 45.0%
!! GTTTTGAGTGGCAGGTTGAA+AGG - chr4.2:31851809-31851828 None:intergenic 45.0%
!! TTGCATTGGCTCCATTGGAT+TGG - chr4.2:31851834-31851853 None:intergenic 45.0%
!!! CCCCTAAGCCAATTTTGACT+CGG - chr4.2:31852901-31852920 None:intergenic 45.0%
!!! GATTGGAAGTTTTGAGTGGC+AGG - chr4.2:31851817-31851836 None:intergenic 45.0%
!!! GGACCATTATTTTGAGTGGC+TGG - chr4.2:31851868-31851887 None:intergenic 45.0%
AACGGGTGGTTGTTGGAACT+GGG - chr4.2:31853370-31853389 None:intergenic 50.0%
AATGCTCAAGGTCAATGGGC+TGG - chr4.2:31853610-31853629 None:intergenic 50.0%
ACGTTGAGAGAAGACGTCAG+AGG - chr4.2:31851751-31851770 None:intergenic 50.0%
AGACCTGGGGAGATATAAGG+TGG - chr4.2:31852531-31852550 None:intergenic 50.0%
AGTTCCAACAACCACCCGTT+GGG + chr4.2:31853370-31853389 MS.gene83178:CDS 50.0%
CAACCACCTTATATCTCCCC+AGG + chr4.2:31852525-31852544 MS.gene83178:intron 50.0%
CAGGAGCAAAACTTAGGGTG+CGG - chr4.2:31852677-31852696 None:intergenic 50.0%
CCAAGTGGAGAATGTGTCTG+TGG + chr4.2:31854127-31854146 MS.gene83178:CDS 50.0%
CCACAGACACATTCTCCACT+TGG - chr4.2:31854130-31854149 None:intergenic 50.0%
CTACTGGTCCTTGTTGAGGT+TGG + chr4.2:31854202-31854221 MS.gene83178:intron 50.0%
CTATTCGGCAGTGGAATCTC+AGG + chr4.2:31853285-31853304 MS.gene83178:CDS 50.0%
CTGCCACAGGAGCAAAACTT+AGG - chr4.2:31852683-31852702 None:intergenic 50.0%
GGAGAAATGGAATCCTGGAG+AGG + chr4.2:31854169-31854188 MS.gene83178:CDS 50.0%
GGAGTATGAGTGGAGGGATA+AGG - chr4.2:31852498-31852517 None:intergenic 50.0%
GGGGAGATATAAGGTGGTTG+AGG - chr4.2:31852525-31852544 None:intergenic 50.0%
GGTTGCATCACACCTCTACA+GGG + chr4.2:31852130-31852149 MS.gene83178:intron 50.0%
GTATGAGTGGAGGGATAAGG+AGG - chr4.2:31852495-31852514 None:intergenic 50.0%
TCGAGACCTGGGGAGATATA+AGG - chr4.2:31852534-31852553 None:intergenic 50.0%
TGACCGAGTCAATTGCCCTA+GGG - chr4.2:31852714-31852733 None:intergenic 50.0%
TGCCACAGGGGCAAAACTTA+GGG + chr4.2:31852928-31852947 MS.gene83178:intron 50.0%
TGCTGCCTGAAACACTGTAG+TGG - chr4.2:31853412-31853431 None:intergenic 50.0%
TGGAACTGGGAAGTAGAACC+TGG - chr4.2:31853357-31853376 None:intergenic 50.0%
TGGAGGGATAAGGAGGTTGT+GGG - chr4.2:31852488-31852507 None:intergenic 50.0%
TGGTTGCATCACACCTCTAC+AGG + chr4.2:31852129-31852148 MS.gene83178:intron 50.0%
TTGACCGAGTCAATTGCCCT+AGG - chr4.2:31852715-31852734 None:intergenic 50.0%
TTGCAGAAATGCGTCCCAAG+TGG + chr4.2:31854112-31854131 MS.gene83178:intron 50.0%
TTGGTAGAGATGCTGGAAGC+AGG + chr4.2:31853899-31853918 MS.gene83178:intron 50.0%
! ATGCTCAAGGTCAATGGGCT+GGG - chr4.2:31853609-31853628 None:intergenic 50.0%
! CACCCTAAGTTTTGCTCCTG+TGG + chr4.2:31852677-31852696 MS.gene83178:intron 50.0%
! CTATCTGGAGGAGCTTGTTC+TGG - chr4.2:31854524-31854543 None:intergenic 50.0%
! GTGTTATTGAACCCAACGGG+TGG - chr4.2:31853384-31853403 None:intergenic 50.0%
! TTGAGGTGTGGGAGTATGAG+TGG - chr4.2:31852508-31852527 None:intergenic 50.0%
! TTTTGCCCCTGTGGCAGTTT+TGG - chr4.2:31852924-31852943 None:intergenic 50.0%
!! CACTGAACCCTGGCTTTGAA+AGG + chr4.2:31851923-31851942 MS.gene83178:CDS 50.0%
!! GGCATTTGCATTGGCTCCAT+TGG - chr4.2:31851839-31851858 None:intergenic 50.0%
AACCCCGCTGGTGTAGGAAA+GGG - chr4.2:31853324-31853343 None:intergenic 55.0%
AACCCCTTTCCTACACCAGC+GGG + chr4.2:31853318-31853337 MS.gene83178:CDS 55.0%
ACCGAGTCAATTGCCCTAGG+GGG - chr4.2:31852712-31852731 None:intergenic 55.0%
CAACCCCTTTCCTACACCAG+CGG + chr4.2:31853317-31853336 MS.gene83178:CDS 55.0%
CAACGGGTGGTTGTTGGAAC+TGG - chr4.2:31853371-31853390 None:intergenic 55.0%
CAGCCTAGTTCCATTGGAGG+TGG + chr4.2:31853139-31853158 MS.gene83178:CDS 55.0%
CAGTTCCAACAACCACCCGT+TGG + chr4.2:31853369-31853388 MS.gene83178:CDS 55.0%
CCAATGGAACTAGGCTGCAC+TGG - chr4.2:31853136-31853155 None:intergenic 55.0%
CCACAGGGGCAAAACTTAGG+GGG + chr4.2:31852930-31852949 MS.gene83178:intron 55.0%
CCAGTGCAGCCTAGTTCCAT+TGG + chr4.2:31853133-31853152 MS.gene83178:CDS 55.0%
CCTAGTTCCATTGGAGGTGG+AGG + chr4.2:31853142-31853161 MS.gene83178:CDS 55.0%
CCTCCACCTCCAATGGAACT+AGG - chr4.2:31853145-31853164 None:intergenic 55.0%
CTGCCACAGGGGCAAAACTT+AGG + chr4.2:31852927-31852946 MS.gene83178:intron 55.0%
GACCGAGTCAATTGCCCTAG+GGG - chr4.2:31852713-31852732 None:intergenic 55.0%
GCCACAGGGGCAAAACTTAG+GGG + chr4.2:31852929-31852948 MS.gene83178:intron 55.0%
GGAAGCAAGTTCGAGACCTG+GGG - chr4.2:31852544-31852563 None:intergenic 55.0%
GGGAAGCAAGTTCGAGACCT+GGG - chr4.2:31852545-31852564 None:intergenic 55.0%
GTAGAGATGCTGGAAGCAGG+CGG + chr4.2:31853902-31853921 MS.gene83178:intron 55.0%
GTGCAGCCTAGTTCCATTGG+AGG + chr4.2:31853136-31853155 MS.gene83178:CDS 55.0%
GTGGAGGGATAAGGAGGTTG+TGG - chr4.2:31852489-31852508 None:intergenic 55.0%
TAGAGATGCTGGAAGCAGGC+GGG + chr4.2:31853903-31853922 MS.gene83178:intron 55.0%
TAGGGGCCAAAACTGCCACA+GGG + chr4.2:31852915-31852934 MS.gene83178:intron 55.0%
TGAACCCAACGGGTGGTTGT+TGG - chr4.2:31853377-31853396 None:intergenic 55.0%
TGGGAAGCAAGTTCGAGACC+TGG - chr4.2:31852546-31852565 None:intergenic 55.0%
TTAGGGGCCAAAACTGCCAC+AGG + chr4.2:31852914-31852933 MS.gene83178:intron 55.0%
TTCGGCAGTGGAATCTCAGG+TGG + chr4.2:31853288-31853307 MS.gene83178:CDS 55.0%
! AGGTGTGGGAGTATGAGTGG+AGG - chr4.2:31852505-31852524 None:intergenic 55.0%
! GGTGTGGGAGTATGAGTGGA+GGG - chr4.2:31852504-31852523 None:intergenic 55.0%
! GGTTCAGTGAAGCACCCAGT+TGG - chr4.2:31851912-31851931 None:intergenic 55.0%
ACCCCGCTGGTGTAGGAAAG+GGG - chr4.2:31853323-31853342 None:intergenic 60.0%
ACCCCTTTCCTACACCAGCG+GGG + chr4.2:31853319-31853338 MS.gene83178:CDS 60.0%
AGGGGCCAAAACTGCCACAG+GGG + chr4.2:31852916-31852935 MS.gene83178:intron 60.0%
CAACCCCGCTGGTGTAGGAA+AGG - chr4.2:31853325-31853344 None:intergenic 60.0%
CAGGGGCAAAACTTAGGGGG+CGG + chr4.2:31852933-31852952 MS.gene83178:intron 60.0%
CTACACCAGCGGGGTTGACA+GGG + chr4.2:31853328-31853347 MS.gene83178:CDS 60.0%
CTGGGTGCTTCACTGAACCC+TGG + chr4.2:31851913-31851932 MS.gene83178:CDS 60.0%
GCCCCCTAGGGCAATTGACT+CGG + chr4.2:31852708-31852727 MS.gene83178:intron 60.0%
TAGGGGGCCAAAACTGCCAC+AGG - chr4.2:31852696-31852715 None:intergenic 60.0%
! CCCCCTAAGTTTTGCCCCTG+TGG - chr4.2:31852933-31852952 None:intergenic 60.0%
!! GTGGCAGTTTTGGCCCCCTA+GGG + chr4.2:31852696-31852715 MS.gene83178:intron 60.0%
!! TGACTCGGTCAACGCACACG+TGG + chr4.2:31852723-31852742 MS.gene83178:intron 60.0%
!! TGACTCGGTCAACGCACACG+TGG - chr4.2:31852742-31852723 None:intergenic 60.0%
!! TGTGGCAGTTTTGGCCCCCT+AGG + chr4.2:31852695-31852714 MS.gene83178:intron 60.0%
CCTACACCAGCGGGGTTGAC+AGG + chr4.2:31853327-31853346 MS.gene83178:CDS 65.0%
CCTGTCAACCCCGCTGGTGT+AGG - chr4.2:31853330-31853349 None:intergenic 65.0%
GCGGGGTTGACAGGGTTTCC+AGG + chr4.2:31853336-31853355 MS.gene83178:CDS 65.0%
GGAAACCCTGTCAACCCCGC+TGG - chr4.2:31853336-31853355 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 31851745 31854556 31851745 ID=MS.gene83178
chr4.2 mRNA 31851745 31854556 31851745 ID=MS.gene83178.t1;Parent=MS.gene83178
chr4.2 exon 31851745 31851944 31851745 ID=MS.gene83178.t1.exon1;Parent=MS.gene83178.t1
chr4.2 CDS 31851745 31851944 31851745 ID=cds.MS.gene83178.t1;Parent=MS.gene83178.t1
chr4.2 exon 31853108 31853163 31853108 ID=MS.gene83178.t1.exon2;Parent=MS.gene83178.t1
chr4.2 CDS 31853108 31853163 31853108 ID=cds.MS.gene83178.t1;Parent=MS.gene83178.t1
chr4.2 exon 31853256 31853445 31853256 ID=MS.gene83178.t1.exon3;Parent=MS.gene83178.t1
chr4.2 CDS 31853256 31853445 31853256 ID=cds.MS.gene83178.t1;Parent=MS.gene83178.t1
chr4.2 exon 31854118 31854190 31854118 ID=MS.gene83178.t1.exon4;Parent=MS.gene83178.t1
chr4.2 CDS 31854118 31854190 31854118 ID=cds.MS.gene83178.t1;Parent=MS.gene83178.t1
chr4.2 exon 31854518 31854556 31854518 ID=MS.gene83178.t1.exon5;Parent=MS.gene83178.t1
chr4.2 CDS 31854518 31854556 31854518 ID=cds.MS.gene83178.t1;Parent=MS.gene83178.t1
Gene Sequence

>MS.gene83178

ATGCCTCTGACGTCTTCTCTCAACGTTCAAAACTTCCAATCCAATGGAGCCAATGTAAATGCCTTTCAACCTGCCACTCAAAACTTCCAATCCAATGGAGCCAATGCAAATGCCTTTCAACCAGCCACTCAAAATAATGGTCCTTTATCAGTCTCAAGCTTTAGCCAACTGGGTGCTTCACTGAACCCTGGCTTTGAAAGGTAACAATGCCATGCTTTAGAACATTTTCTTCATTTTCAATTGTGTGGAGTCAAAAAATGACCTAAATCAAGAAATATCCAAATTTTAGCTAACAGGCAAAGCAAAGAGCTTAACTGCTATACGGTTTGCAATCGTATAGAGATATTGACGAGGCCTGATTGTTTGTGACCTACTTTCTTTAGTTGGTTGCATCACACCTCTACAGGGATTCCTTTTTCTACAACTGCTATCTACTATTTGTTTTTGTTTGGGATTATGGTGTGATTGTCAAAAGGGTGGGGAAAGACATGTATCTTTCTATTATAGTGGTTTAAGGTTACAGTTTCAGAAATGTTAATACATACTAGTTGAATATCTGTTGCAAGTTCTGTTGAATGACCTAGGTCATGAGAGTTTGGTGTCCGATTACATACCTAAAATGATGGTTTTCTTATGTTTGGCATTGAGTGATTCAAAATCAGTGAATTTTGTTTTTTTGAATGGCAAATTCTAGCACGTTAGCTGTTAATGTTAGTTTTTTGTTGTGGGTGGGGGTTGAACCCACAACCTCCTTATCCCTCCACTCATACTCCCACACCTCAACCACCTTATATCTCCCCAGGTCTCGAACTTGCTTCCCATTCAATGTTTATATCTTGTTCCTTTATTTTATAGGCTTAATAGCAGTTTTAGCCCCCTAAGTTTCAAGAAGTTGCGATTTTGGCCCCCTAAGAAAAAAAACTACAAAACCGCACCCTAAGTTTTGCTCCTGTGGCAGTTTTGGCCCCCTAGGGCAATTGACTCGGTCAACGCACACGTGGACGCCGTGTGTATTTTTTTAAAAAAATAAATAAACAAATCTTTTTTTATTTTTTAATAAAAATATTAAAAAAATATTTTTTAATTACAAAAATAAAAAAAATAATTTTTAAATAATTAAAAAAAATACACGTGGCATCCACGTGTGCGTTGACCGAGTCAAAATTGGCTTAGGGGCCAAAACTGCCACAGGGGCAAAACTTAGGGGGCGGTTTTGTAGTTTTTTTTCTTAAGGGGCCAAAATCGCAACTTCTTGAAACTTAGGGGGCTAAAACTGCTATTAAGCCTATTTTATATTCTCATGATTTTTTTTTTTTATGTCTGCTCTTGGCATATGGTAATCTGTTTGTAACTTTCTTGTTAGGCCCTCTGCACCATTAATGAGTACTCCAGTGCAGCCTAGTTCCATTGGAGGTGGAGGTATGTATTTTGCGAAATTTCGATGACAAACACTATAAGTACATTTGTCTTCTATTTTCCTTGTTTGACCATGATGTTGTGATAAACTGTAGGAAACTTTTTCACACCCAACTCAGGAAATCTATTCGGCAGTGGAATCTCAGGTGGTCAAATCAACCCCTTTCCTACACCAGCGGGGTTGACAGGGTTTCCAGGTTCTACTTCCCAGTTCCAACAACCACCCGTTGGGTTCAATAACACTAGCTCCACTACAGTGTTTCAGGCAGCACCAGATGTTCAGTTGTAAGTCACTGCTCACACTAGTTTAAAGTTCTAAAATAGTAGCTGTGAGCCTGTGACAATTTAATAAAATCCATTTTCATTCAATAGTCAACCAAAAATAGTTGTACAACTTTCTAGAAAACAGAGTTCAGTTAATACGATATAGATCATAACGACATAACCCAGCCCATTGACCTTGAGCATTTTCAGATAGTTGCATCTTCAAATTAGTTATGTATTTTCAATTTGAGCAGATCTCCTGACACTCTTATGTAATTCACTTTTGTGATGAAACTCATAATTGATACTCTTGTTAATATGAGAAAATTCATGTTAATTTCCCTACAAAGTAAATGACCCAATTGCACCAGTTATAAATGTTATAATTTGGACAAAGTTGAGTGCATATATCAGTGATAAAGTCATTTCAGTATAGCCATATGTTACAGTTTAAAGATTAAGAAGATTTTAATGTTGGTAGAGATGCTGGAAGCAGGCGGGTCTTTTGTTTGCTTTAATTATTACTAGTTTTGAACAAAGCCTCCAACTTTTGTTAGTATTGAACCTACTTGGAAACTTCACCTTTGTTACTGCACTAACTTTCTGGGGCTATCTTTATAGAGCTTTTAATTCAGCTTCATGTGCAAGTTACAAATACTTTTTCTTGAATCTTATGGTTACCTATTCTTGCAGAAATGCGTCCCAAGTGGAGAATGTGTCTGTGGATGATAGTATCTGGTTAAAGGAGAAATGGAATCCTGGAGAGGTAATAGTCTTCTACTGGTCCTTGTTGAGGTTGGGAATAAGTGTCTATTCAGGTTTCTCAAAAACATTTATTTCATTTACAAAACCATGACAAAATTTCCTTTGTGATACATACATACATAGTATTGGATAGAATCTGCAATAGGATCTTTTGGTTCTTGCATGTGCAAATATACAATTCGTCGTCTCCGAGCATTAAAATTTGGCAGAAATATGCTTACTTTTTGTTGACTATATATTTTAAAATTCAAGTGTTGGGGATATGTTTTCTCAATTTGTGAGATATATGCTAACATTTTTCTCTGTTTCATGTCTTTTAATTTGACAGATTCCAGAACAAGCTCCTCCAGATAGATTTGTTTGGTAG
Protein sequence

>MS.gene83178.t1

MPLTSSLNVQNFQSNGANVNAFQPATQNFQSNGANANAFQPATQNNGPLSVSSFSQLGASLNPGFERPSAPLMSTPVQPSSIGGGGNFFTPNSGNLFGSGISGGQINPFPTPAGLTGFPGSTSQFQQPPVGFNNTSSTTVFQAAPDVQLNASQVENVSVDDSIWLKEKWNPGEIPEQAPPDRFVW