Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83420.t1 | XP_013446290.1 | 80.1 | 699 | 31 | 1 | 3 | 593 | 4 | 702 | 2.00E-294 | 1021.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83420.t1 | Q9LYG9 | 47.3 | 482 | 239 | 6 | 112 | 583 | 258 | 734 | 8.4e-108 | 392.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83420.t1 | A0A072TSB8 | 80.1 | 699 | 31 | 1 | 3 | 593 | 4 | 702 | 1.4e-294 | 1021.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050657 | MS.gene83420 | 0.802788 | 4.75E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83420.t1 | MTR_8g071250 | 99.174 | 484 | 4 | 0 | 110 | 593 | 219 | 702 | 0.0 | 996 |
| MS.gene83420.t1 | MTR_8g071250 | 90.826 | 109 | 10 | 0 | 3 | 111 | 4 | 112 | 1.20e-60 | 214 |
| MS.gene83420.t1 | MTR_2g041220 | 52.049 | 488 | 218 | 7 | 110 | 586 | 271 | 753 | 1.47e-170 | 503 |
| MS.gene83420.t1 | MTR_3g104940 | 47.531 | 486 | 232 | 11 | 110 | 582 | 289 | 764 | 3.84e-142 | 431 |
| MS.gene83420.t1 | MTR_3g104920 | 47.648 | 489 | 221 | 10 | 110 | 579 | 254 | 726 | 4.35e-141 | 427 |
| MS.gene83420.t1 | MTR_3g104920 | 47.648 | 489 | 221 | 10 | 110 | 579 | 266 | 738 | 6.30e-141 | 427 |
| MS.gene83420.t1 | MTR_3g104990 | 48.148 | 486 | 229 | 10 | 110 | 582 | 288 | 763 | 6.91e-139 | 422 |
| MS.gene83420.t1 | MTR_1g113850 | 41.243 | 531 | 257 | 7 | 110 | 589 | 391 | 917 | 1.79e-122 | 384 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83420.t1 | AT5G12080 | 46.888 | 482 | 241 | 7 | 112 | 583 | 258 | 734 | 2.59e-148 | 445 |
| MS.gene83420.t1 | AT5G12080 | 46.888 | 482 | 241 | 7 | 112 | 583 | 258 | 734 | 2.59e-148 | 445 |
| MS.gene83420.t1 | AT5G12080 | 46.888 | 482 | 241 | 7 | 112 | 583 | 258 | 734 | 2.59e-148 | 445 |
| MS.gene83420.t1 | AT5G19520 | 44.167 | 480 | 248 | 11 | 111 | 580 | 270 | 739 | 9.12e-131 | 400 |
| MS.gene83420.t1 | AT5G19520 | 44.167 | 480 | 248 | 11 | 111 | 580 | 270 | 739 | 9.12e-131 | 400 |
| MS.gene83420.t1 | AT1G53470 | 42.330 | 515 | 253 | 9 | 110 | 582 | 348 | 860 | 1.51e-127 | 396 |
| MS.gene83420.t1 | AT3G14810 | 42.970 | 505 | 253 | 8 | 110 | 582 | 358 | 859 | 9.21e-126 | 391 |
| MS.gene83420.t1 | AT2G17010 | 42.292 | 506 | 255 | 10 | 110 | 582 | 389 | 890 | 2.13e-121 | 380 |
| MS.gene83420.t1 | AT1G78610 | 42.406 | 507 | 253 | 8 | 110 | 582 | 331 | 832 | 4.22e-121 | 379 |
| MS.gene83420.t1 | AT2G17000 | 42.885 | 506 | 252 | 11 | 110 | 582 | 322 | 823 | 1.05e-118 | 372 |
| MS.gene83420.t1 | AT2G17000 | 42.885 | 506 | 252 | 11 | 110 | 582 | 322 | 823 | 1.26e-118 | 372 |
| MS.gene83420.t1 | AT3G14810 | 39.883 | 514 | 259 | 11 | 113 | 582 | 317 | 824 | 2.06e-107 | 342 |
| MS.gene83420.t1 | AT2G17010 | 37.786 | 262 | 126 | 10 | 110 | 338 | 389 | 646 | 9.34e-36 | 143 |
Find 112 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCAGATCTTCCTTTAGAAAT+TGG | 0.137575 | 8.4:+32369607 | MS.gene83420:CDS |
| TCTAGGACTATTGATGATTT+TGG | 0.142127 | 8.4:+32366254 | MS.gene83420:CDS |
| TCAATTGCATCACTCATTTC+TGG | 0.158373 | 8.4:-32371308 | None:intergenic |
| ACACCTTGTTGAGGAATTTA+TGG | 0.163154 | 8.4:-32365857 | None:intergenic |
| GTTAATTATGTTAGGTCTTC+AGG | 0.186545 | 8.4:+32366218 | MS.gene83420:CDS |
| TGGTTCGAGGAGGATTGATA+TGG | 0.264557 | 8.4:+32366136 | MS.gene83420:CDS |
| ACCATAATTCTGGTGGTTAA+TGG | 0.274430 | 8.4:-32371914 | None:intergenic |
| CAGGTTTGTCAACAATTTCT+AGG | 0.287188 | 8.4:+32366237 | MS.gene83420:CDS |
| CATCCTCTGATTCTTCTTCC+TGG | 0.298721 | 8.4:-32365443 | None:intergenic |
| CATCTCACTCAAATGAATAT+TGG | 0.302722 | 8.4:+32372030 | MS.gene83420:CDS |
| TCTGGTCCACCGGTCGAAAA+TGG | 0.304486 | 8.4:+32366029 | MS.gene83420:CDS |
| ACCATTAACCACCAGAATTA+TGG | 0.311068 | 8.4:+32371913 | MS.gene83420:CDS |
| GTATTCAAGGATTCGAGATT+TGG | 0.323814 | 8.4:-32365316 | None:intergenic |
| CAACCCTAAACCTTCTTCTA+TGG | 0.328740 | 8.4:+32365200 | MS.gene83420:CDS |
| GTGTCCATAGAAGAAGGTTT+AGG | 0.335239 | 8.4:-32365204 | None:intergenic |
| TGCCGCCGGGTGTGGGGAGG+TGG | 0.340409 | 8.4:+32366096 | MS.gene83420:CDS |
| TACTTCTCTCACCATAATTC+TGG | 0.340683 | 8.4:-32371924 | None:intergenic |
| TACTTACTTGGCACCAGGTT+TGG | 0.348312 | 8.4:-32366348 | None:intergenic |
| GGAAGATCTGCTAATCTTCC+CGG | 0.350454 | 8.4:-32369595 | None:intergenic |
| TTTGAAGGTGCTCTTGAAAC+CGG | 0.352027 | 8.4:+32369576 | MS.gene83420:CDS |
| CAGATCTTCCTTTAGAAATT+GGG | 0.353026 | 8.4:+32369608 | MS.gene83420:CDS |
| AAGTTGTTGCACTGCTGTTT+TGG | 0.353295 | 8.4:-32369857 | None:intergenic |
| TGGAAGAAGATGAAGAGATT+TGG | 0.355511 | 8.4:+32366116 | MS.gene83420:CDS |
| AAAAGCTTGTTAATTATGTT+AGG | 0.357821 | 8.4:+32366210 | MS.gene83420:CDS |
| TGGAGAAGCTTAGACGGTTA+AGG | 0.358941 | 8.4:-32365290 | None:intergenic |
| TGTCCATAGAAGAAGGTTTA+GGG | 0.361650 | 8.4:-32365203 | None:intergenic |
| GAAGTTGAAGAAACTTAGTA+TGG | 0.366233 | 8.4:+32366160 | MS.gene83420:CDS |
| TTTGCCATCACACTATGCTT+TGG | 0.367635 | 8.4:-32371841 | None:intergenic |
| AATAATAATTGTGACCCTTT+TGG | 0.369704 | 8.4:+32369912 | MS.gene83420:CDS |
| TTTGCTCTAGAGGGATGTTT+AGG | 0.372443 | 8.4:-32365264 | None:intergenic |
| TTGGTTAGTGAAAATTGTTT+TGG | 0.374026 | 8.4:+32365917 | MS.gene83420:CDS |
| GGAGAAGCTTAGACGGTTAA+GGG | 0.374557 | 8.4:-32365289 | None:intergenic |
| AAGGTTTAGGGTTGTGTTGT+TGG | 0.374758 | 8.4:-32365191 | None:intergenic |
| ACCTTCAAAGAGTGGAAATA+TGG | 0.382658 | 8.4:-32369562 | None:intergenic |
| TATTCAAGGATTCGAGATTT+GGG | 0.392191 | 8.4:-32365315 | None:intergenic |
| TGCTTTGGATTCCAATACTT+TGG | 0.400245 | 8.4:-32371826 | None:intergenic |
| TGGAGAGTAGTGGTTCTGTT+TGG | 0.408314 | 8.4:+32366180 | MS.gene83420:CDS |
| AGATGAAGAGATTTGGTTCG+AGG | 0.412885 | 8.4:+32366123 | MS.gene83420:CDS |
| TATGTGTTGGATGCATTGTC+TGG | 0.417172 | 8.4:+32366011 | MS.gene83420:CDS |
| AGGCTTCTCTTGATGAGGTT+CGG | 0.417205 | 8.4:-32365244 | None:intergenic |
| ACCATATTTCCACTCTTTGA+AGG | 0.420079 | 8.4:+32369561 | MS.gene83420:CDS |
| AAAGACGATGCCGCCGGGTG+TGG | 0.420836 | 8.4:+32366088 | MS.gene83420:CDS |
| TTAGTGAGCAAGATTGCGTT+AGG | 0.423646 | 8.4:-32371260 | None:intergenic |
| ACTCAAATGAATATTGGAAA+TGG | 0.435631 | 8.4:+32372036 | MS.gene83420:CDS |
| GAAGAGAATAACTTGAGGTT+CGG | 0.441585 | 8.4:-32365163 | None:intergenic |
| AAGGATTCGAGATTTGGGTT+TGG | 0.441587 | 8.4:-32365310 | None:intergenic |
| CAACGGTCTCCAATATCAAA+AGG | 0.442822 | 8.4:-32370060 | None:intergenic |
| TTCAACAATGTTGCCAAACC+TGG | 0.445464 | 8.4:+32366335 | MS.gene83420:CDS |
| GAGGTGGATGGTTGTATTCA+AGG | 0.446525 | 8.4:-32365329 | None:intergenic |
| ATCGAAAGAGCATCCTTTGA+AGG | 0.453490 | 8.4:+32366061 | MS.gene83420:CDS |
| CAATATTCATTTGAGTGAGA+TGG | 0.454096 | 8.4:-32372029 | None:intergenic |
| GAGTGAGATGGATTTCTTGA+GGG | 0.454915 | 8.4:-32372017 | None:intergenic |
| GGATTTCTTGAGGGAGAAGA+TGG | 0.463123 | 8.4:-32372008 | None:intergenic |
| TAGGGTTGTGTTGTTGGTCA+AGG | 0.463940 | 8.4:-32365185 | None:intergenic |
| AGGCACTTGCAAAACTGTCT+TGG | 0.470534 | 8.4:+32370008 | MS.gene83420:CDS |
| AGTGCAATGATAGTCTCTAT+AGG | 0.476490 | 8.4:-32371353 | None:intergenic |
| AGAGTGAAATTAGTAGTGAA+TGG | 0.480411 | 8.4:+32366285 | MS.gene83420:CDS |
| TAGGATATCAGAACTCCTCT+TGG | 0.481998 | 8.4:+32371948 | MS.gene83420:CDS |
| TTGAGAGCAAACCAAAGTAT+TGG | 0.485327 | 8.4:+32371815 | MS.gene83420:CDS |
| TAGCAACTTCCATCACCAAA+AGG | 0.489311 | 8.4:-32369927 | None:intergenic |
| AAGACGATGCCGCCGGGTGT+GGG | 0.491957 | 8.4:+32366089 | MS.gene83420:CDS |
| ACTTACCACCCAATTTCTAA+AGG | 0.492720 | 8.4:-32369616 | None:intergenic |
| CTTGATGAGGTTCGGTGTTG+TGG | 0.494184 | 8.4:-32365236 | None:intergenic |
| GTTTCAGTGTCCATAGAAGA+AGG | 0.503056 | 8.4:-32365210 | None:intergenic |
| CGATGCCGCCGGGTGTGGGG+AGG | 0.503432 | 8.4:+32366093 | MS.gene83420:CDS |
| TATAGGAGTTGTAACATCAA+TGG | 0.509480 | 8.4:-32371336 | None:intergenic |
| GTGTCTTCTTCTTCAGAGGT+AGG | 0.512031 | 8.4:-32365363 | None:intergenic |
| TTGAAGGTGCTCTTGAAACC+GGG | 0.516410 | 8.4:+32369577 | MS.gene83420:CDS |
| TTGTGTCATTGACGGTGTCC+AGG | 0.516753 | 8.4:+32370080 | MS.gene83420:CDS |
| CGATAGCGACTATGAATACC+AGG | 0.518235 | 8.4:+32365425 | MS.gene83420:CDS |
| GGATGCATTGTCTGGTCCAC+CGG | 0.518479 | 8.4:+32366019 | MS.gene83420:CDS |
| TGTTTAGGCTTCTCTTGATG+AGG | 0.519840 | 8.4:-32365249 | None:intergenic |
| AGACGATGCCGCCGGGTGTG+GGG | 0.528623 | 8.4:+32366090 | MS.gene83420:CDS |
| GGAAGAAGAATCAGAGGATG+AGG | 0.528727 | 8.4:+32365446 | MS.gene83420:CDS |
| GGCAAAACAAATAGATCAAG+TGG | 0.534255 | 8.4:+32371858 | MS.gene83420:CDS |
| GGGTTCAAAGACGATGCCGC+CGG | 0.536140 | 8.4:+32366082 | MS.gene83420:CDS |
| GTAGGTAGTTTGTTGCGAGG+TGG | 0.543035 | 8.4:-32365345 | None:intergenic |
| TGAAGAGATTTGGTTCGAGG+AGG | 0.543710 | 8.4:+32366126 | MS.gene83420:CDS |
| GGAGACCGTTGTGTCATTGA+CGG | 0.544383 | 8.4:+32370072 | MS.gene83420:CDS |
| TTCTTCCACCTCCCCACACC+CGG | 0.545113 | 8.4:-32366101 | None:intergenic |
| TTAATAGGAGTTGCTTTCCA+AGG | 0.546937 | 8.4:+32369988 | MS.gene83420:CDS |
| ATCGTCTTTGAACCCTTCAA+AGG | 0.549358 | 8.4:-32366074 | None:intergenic |
| GTAGTTTGTTGCGAGGTGGA+TGG | 0.556267 | 8.4:-32365341 | None:intergenic |
| TTGAGTGTCTTCTTCTTCAG+AGG | 0.558346 | 8.4:-32365367 | None:intergenic |
| AACGGTCTCCAATATCAAAA+GGG | 0.561853 | 8.4:-32370059 | None:intergenic |
| GGTTCAAAGACGATGCCGCC+GGG | 0.570113 | 8.4:+32366083 | MS.gene83420:CDS |
| ATCTTCCTTTAGAAATTGGG+TGG | 0.572304 | 8.4:+32369611 | MS.gene83420:CDS |
| TAATTATTTACAGATGATTG+TGG | 0.572993 | 8.4:+32371178 | MS.gene83420:intron |
| TCGAAAGAGCATCCTTTGAA+GGG | 0.574957 | 8.4:+32366062 | MS.gene83420:CDS |
| AAATATACTTACTTGGCACC+AGG | 0.575137 | 8.4:-32366353 | None:intergenic |
| AGCAACTTCCATCACCAAAA+GGG | 0.577274 | 8.4:-32369926 | None:intergenic |
| TCAGAAGAGATCTTGTATGT+AGG | 0.583061 | 8.4:-32365393 | None:intergenic |
| TGGGTTTGGAGAAGCTTAGA+CGG | 0.588604 | 8.4:-32365296 | None:intergenic |
| TGAGTGAGATGGATTTCTTG+AGG | 0.589719 | 8.4:-32372018 | None:intergenic |
| TTCTCTCACCATAATTCTGG+TGG | 0.590623 | 8.4:-32371921 | None:intergenic |
| TCAAGAGCACCTTCAAAGAG+TGG | 0.591890 | 8.4:-32369570 | None:intergenic |
| CATGATGAAGACAAAGATGA+TGG | 0.594026 | 8.4:-32370034 | None:intergenic |
| GAATCCAAAGCATAGTGTGA+TGG | 0.621159 | 8.4:+32371837 | MS.gene83420:CDS |
| ATACCAGGAAGAAGAATCAG+AGG | 0.622604 | 8.4:+32365440 | MS.gene83420:CDS |
| TAGTGAGCAAGATTGCGTTA+GGG | 0.638961 | 8.4:-32371259 | None:intergenic |
| GAGGTAGGTAGTTTGTTGCG+AGG | 0.642897 | 8.4:-32365348 | None:intergenic |
| ATCAGAGGATGAGGACGACG+AGG | 0.643308 | 8.4:+32365455 | MS.gene83420:CDS |
| GTGCTTGAAACACCTTGTTG+AGG | 0.657441 | 8.4:-32365866 | None:intergenic |
| ATTATGGTGAGAGAAGTACT+AGG | 0.657963 | 8.4:+32371929 | MS.gene83420:CDS |
| AAACTTAGTATGGAGAGTAG+TGG | 0.659135 | 8.4:+32366170 | MS.gene83420:CDS |
| TCAGAGGATGAGGACGACGA+GGG | 0.689343 | 8.4:+32365456 | MS.gene83420:CDS |
| TCCACCGGTCGAAAATGGCG+AGG | 0.689381 | 8.4:+32366034 | MS.gene83420:CDS |
| TCAAGGAAGAGAATAACTTG+AGG | 0.689798 | 8.4:-32365168 | None:intergenic |
| AAACCATAAATTCCTCAACA+AGG | 0.693061 | 8.4:+32365854 | MS.gene83420:CDS |
| GGACACCGTCAATGACACAA+CGG | 0.732774 | 8.4:-32370077 | None:intergenic |
| TTCCACCTCCCCACACCCGG+CGG | 0.733208 | 8.4:-32366098 | None:intergenic |
| GTCATATCGAAGAAACACTG+TGG | 0.772230 | 8.4:-32371219 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 32365150 | 32372080 | 32365150 | ID=MS.gene83420 |
| chr8.4 | mRNA | 32365150 | 32372080 | 32365150 | ID=MS.gene83420.t1;Parent=MS.gene83420 |
| chr8.4 | exon | 32365150 | 32365477 | 32365150 | ID=MS.gene83420.t1.exon1;Parent=MS.gene83420.t1 |
| chr8.4 | CDS | 32365150 | 32365477 | 32365150 | ID=cds.MS.gene83420.t1;Parent=MS.gene83420.t1 |
| chr8.4 | exon | 32365802 | 32366363 | 32365802 | ID=MS.gene83420.t1.exon2;Parent=MS.gene83420.t1 |
| chr8.4 | CDS | 32365802 | 32366363 | 32365802 | ID=cds.MS.gene83420.t1;Parent=MS.gene83420.t1 |
| chr8.4 | exon | 32369509 | 32369632 | 32369509 | ID=MS.gene83420.t1.exon3;Parent=MS.gene83420.t1 |
| chr8.4 | CDS | 32369509 | 32369632 | 32369509 | ID=cds.MS.gene83420.t1;Parent=MS.gene83420.t1 |
| chr8.4 | exon | 32369808 | 32370101 | 32369808 | ID=MS.gene83420.t1.exon4;Parent=MS.gene83420.t1 |
| chr8.4 | CDS | 32369808 | 32370101 | 32369808 | ID=cds.MS.gene83420.t1;Parent=MS.gene83420.t1 |
| chr8.4 | exon | 32371191 | 32371393 | 32371191 | ID=MS.gene83420.t1.exon5;Parent=MS.gene83420.t1 |
| chr8.4 | CDS | 32371191 | 32371393 | 32371191 | ID=cds.MS.gene83420.t1;Parent=MS.gene83420.t1 |
| chr8.4 | exon | 32371810 | 32372080 | 32371810 | ID=MS.gene83420.t1.exon6;Parent=MS.gene83420.t1 |
| chr8.4 | CDS | 32371810 | 32372080 | 32371810 | ID=cds.MS.gene83420.t1;Parent=MS.gene83420.t1 |
| Gene Sequence |
| Protein sequence |
