Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83428.t1 | XP_013449506.1 | 99.3 | 135 | 1 | 0 | 1 | 135 | 83 | 217 | 1.30E-68 | 268.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene83428.t1 | A0A072U1E7 | 99.3 | 135 | 1 | 0 | 1 | 135 | 83 | 217 | 9.5e-69 | 268.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058081 | MS.gene83428 | 0.805044 | 1.61E-49 | -1.69E-46 |
| MS.gene059283 | MS.gene83428 | 0.804824 | 1.79E-49 | -1.69E-46 |
| MS.gene06052 | MS.gene83428 | 0.829954 | 3.85E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 34 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGCTTCTTTGGATGATTT+TGG | 0.165257 | 7.2:-22125510 | None:intergenic |
| AACTTACCAATAAGCTTCTT+TGG | 0.237998 | 7.2:-22125520 | None:intergenic |
| AATCATCCAAAGAAGCTTAT+TGG | 0.258024 | 7.2:+22125514 | MS.gene83428:CDS |
| AGGAAGCTTACAACCTTTCA+TGG | 0.340391 | 7.2:-22125468 | None:intergenic |
| GTCAAGAGAGATATGAAGTT+AGG | 0.367836 | 7.2:+22125702 | MS.gene83428:CDS |
| TCAAGAGAGATATGAAGTTA+GGG | 0.417350 | 7.2:+22125703 | MS.gene83428:CDS |
| TGTTTACAGTTGGTAAGTCC+TGG | 0.420464 | 7.2:+22125659 | MS.gene83428:intron |
| CCCATAGCCTTCAATCCTCT+TGG | 0.424567 | 7.2:-22125853 | None:intergenic |
| GGCCGGAGATAACAGAAAAT+CGG | 0.460576 | 7.2:+22125286 | MS.gene83428:CDS |
| CCAAGAGGATTGAAGGCTAT+GGG | 0.481069 | 7.2:+22125853 | MS.gene83428:CDS |
| GCAGAGGCCAAGAGGATTGA+AGG | 0.486507 | 7.2:+22125846 | MS.gene83428:intron |
| GCCAAGAGGATTGAAGGCTA+TGG | 0.494932 | 7.2:+22125852 | MS.gene83428:CDS |
| AACATCGCAAGTGCCTTTAC+CGG | 0.498467 | 7.2:-22125363 | None:intergenic |
| TCAGCAGAGGACACCGGTAA+AGG | 0.516208 | 7.2:+22125350 | MS.gene83428:CDS |
| GGAGAAGAAAAGTAACAAGA+AGG | 0.520874 | 7.2:+22125724 | MS.gene83428:CDS |
| GAGAGAGTAATGTAAAGCTT+AGG | 0.521687 | 7.2:-22125416 | None:intergenic |
| ATTGAAGGCTATGGGGAGTG+TGG | 0.522393 | 7.2:+22125861 | MS.gene83428:CDS |
| GGCACTTGCGATGTTATCAA+TGG | 0.530662 | 7.2:+22125371 | MS.gene83428:CDS |
| TAACAGAAAATCGGCATCAC+CGG | 0.532579 | 7.2:+22125295 | MS.gene83428:CDS |
| ATATCAGTCAACCATGCTCC+AGG | 0.532665 | 7.2:-22125677 | None:intergenic |
| AAAATCGGCATCACCGGAGA+AGG | 0.542456 | 7.2:+22125301 | MS.gene83428:CDS |
| TTGGTATGCAGGAATTCCGA+TGG | 0.546689 | 7.2:+22125245 | None:intergenic |
| TAGTATCGATCTTCCTTCTC+CGG | 0.549815 | 7.2:-22125314 | None:intergenic |
| ACTATTCTACGACCGGCGGC+CGG | 0.561495 | 7.2:+22125269 | MS.gene83428:CDS |
| CGATGGAACTATTCTACGAC+CGG | 0.568292 | 7.2:+22125262 | MS.gene83428:CDS |
| AGTTGGTAAGTCCTGGAGCA+TGG | 0.569217 | 7.2:+22125666 | MS.gene83428:intron |
| TTTCTGTTATCTCCGGCCGC+CGG | 0.574206 | 7.2:-22125281 | None:intergenic |
| CGGTCGTAGAATAGTTCCAT+CGG | 0.626224 | 7.2:-22125261 | None:intergenic |
| AGATCGATACTACACGACGA+GGG | 0.631631 | 7.2:+22125325 | MS.gene83428:CDS |
| GATCGATACTACACGACGAG+GGG | 0.647579 | 7.2:+22125326 | MS.gene83428:CDS |
| CAAGAGGATTGAAGGCTATG+GGG | 0.656812 | 7.2:+22125854 | MS.gene83428:CDS |
| ATCGATACTACACGACGAGG+GGG | 0.671513 | 7.2:+22125327 | MS.gene83428:CDS |
| TGGAACTATTCTACGACCGG+CGG | 0.694045 | 7.2:+22125265 | MS.gene83428:CDS |
| AAGATCGATACTACACGACG+AGG | 0.699452 | 7.2:+22125324 | MS.gene83428:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAGAGATAAAATAAAAA+AGG | - | chr7.2:22125789-22125808 | None:intergenic | 10.0% |
| !!! | TTTTTATGTTGTTTTTATTA+TGG | + | chr7.2:22125565-22125584 | MS.gene83428:intron | 10.0% |
| !!! | TGTTTTTATTATGGTTAATT+GGG | + | chr7.2:22125574-22125593 | MS.gene83428:intron | 15.0% |
| !!! | TTGTTTTTATTATGGTTAAT+TGG | + | chr7.2:22125573-22125592 | MS.gene83428:intron | 15.0% |
| ! | TTATTATGGTTAATTGGGTT+TGG | + | chr7.2:22125579-22125598 | MS.gene83428:intron | 25.0% |
| AAAAGTAACAAGAAGGTAAG+TGG | + | chr7.2:22125731-22125750 | MS.gene83428:intron | 30.0% | |
| AACTTACCAATAAGCTTCTT+TGG | - | chr7.2:22125523-22125542 | None:intergenic | 30.0% | |
| AATCATCCAAAGAAGCTTAT+TGG | + | chr7.2:22125514-22125533 | MS.gene83428:CDS | 30.0% | |
| TCAAGAGAGATATGAAGTTA+GGG | + | chr7.2:22125703-22125722 | MS.gene83428:CDS | 30.0% | |
| ! | TAAGCTTCTTTGGATGATTT+TGG | - | chr7.2:22125513-22125532 | None:intergenic | 30.0% |
| ACAAACACCAAACAAACATG+TGG | - | chr7.2:22125642-22125661 | None:intergenic | 35.0% | |
| ATGTTGTCCACATGTTTGTT+TGG | + | chr7.2:22125632-22125651 | MS.gene83428:intron | 35.0% | |
| GAGAAGAAAAAGCTCTTGTT+TGG | + | chr7.2:22125393-22125412 | MS.gene83428:CDS | 35.0% | |
| GAGAGAGTAATGTAAAGCTT+AGG | - | chr7.2:22125419-22125438 | None:intergenic | 35.0% | |
| GGAGAAGAAAAGTAACAAGA+AGG | + | chr7.2:22125724-22125743 | MS.gene83428:CDS | 35.0% | |
| GTCAAGAGAGATATGAAGTT+AGG | + | chr7.2:22125702-22125721 | MS.gene83428:CDS | 35.0% | |
| ! | GAAGATTTTCTTGCCATGAA+AGG | + | chr7.2:22125455-22125474 | MS.gene83428:CDS | 35.0% |
| !! | ATGATTTTGGCCCTCTTTTT+GGG | - | chr7.2:22125500-22125519 | None:intergenic | 35.0% |
| !! | GTTTGGTGTTTGTTTACAGT+TGG | + | chr7.2:22125649-22125668 | MS.gene83428:intron | 35.0% |
| !!! | TTTTTGGGTTTTTGATGCAG+AGG | + | chr7.2:22125830-22125849 | MS.gene83428:intron | 35.0% |
| ACAAGAAGGTAAGTGGTTGT+TGG | + | chr7.2:22125738-22125757 | MS.gene83428:intron | 40.0% | |
| AGGAAGCTTACAACCTTTCA+TGG | - | chr7.2:22125471-22125490 | None:intergenic | 40.0% | |
| TAACAGAAAATCGGCATCAC+CGG | + | chr7.2:22125295-22125314 | MS.gene83428:CDS | 40.0% | |
| TCCTCAAAGACCCAAAAAGA+GGG | + | chr7.2:22125487-22125506 | MS.gene83428:CDS | 40.0% | |
| TTCCTCAAAGACCCAAAAAG+AGG | + | chr7.2:22125486-22125505 | MS.gene83428:CDS | 40.0% | |
| !! | GATGATTTTGGCCCTCTTTT+TGG | - | chr7.2:22125501-22125520 | None:intergenic | 40.0% |
| !! | TAGTATCGATCTTCCTTCTC+CGG | - | chr7.2:22125317-22125336 | None:intergenic | 40.0% |
| !! | TGTTTACAGTTGGTAAGTCC+TGG | + | chr7.2:22125659-22125678 | MS.gene83428:intron | 40.0% |
| !!! | TGCCGATTTTCTGTTATCTC+CGG | - | chr7.2:22125291-22125310 | None:intergenic | 40.0% |
| AACATCGCAAGTGCCTTTAC+CGG | - | chr7.2:22125366-22125385 | None:intergenic | 45.0% | |
| AAGATCGATACTACACGACG+AGG | + | chr7.2:22125324-22125343 | MS.gene83428:CDS | 45.0% | |
| AGATCGATACTACACGACGA+GGG | + | chr7.2:22125325-22125344 | MS.gene83428:CDS | 45.0% | |
| ATATCAGTCAACCATGCTCC+AGG | - | chr7.2:22125680-22125699 | None:intergenic | 45.0% | |
| CAAGAGGATTGAAGGCTATG+GGG | + | chr7.2:22125854-22125873 | MS.gene83428:CDS | 45.0% | |
| CCAAGAGGATTGAAGGCTAT+GGG | + | chr7.2:22125853-22125872 | MS.gene83428:CDS | 45.0% | |
| CGATGGAACTATTCTACGAC+CGG | + | chr7.2:22125262-22125281 | MS.gene83428:CDS | 45.0% | |
| CGGTCGTAGAATAGTTCCAT+CGG | - | chr7.2:22125264-22125283 | None:intergenic | 45.0% | |
| GGCACTTGCGATGTTATCAA+TGG | + | chr7.2:22125371-22125390 | MS.gene83428:CDS | 45.0% | |
| GGCCGGAGATAACAGAAAAT+CGG | + | chr7.2:22125286-22125305 | MS.gene83428:CDS | 45.0% | |
| ! | TTTTTGATGCAGAGGCCAAG+AGG | + | chr7.2:22125838-22125857 | MS.gene83428:intron | 45.0% |
| !!! | TTTTTCTAAATATAATTTTT+GGG | + | chr7.2:22125815-22125834 | MS.gene83428:intron | 5.0% |
| !!! | TTTTTTCTAAATATAATTTT+TGG | + | chr7.2:22125814-22125833 | MS.gene83428:intron | 5.0% |
| AAAATCGGCATCACCGGAGA+AGG | + | chr7.2:22125301-22125320 | MS.gene83428:CDS | 50.0% | |
| ATCGATACTACACGACGAGG+GGG | + | chr7.2:22125327-22125346 | MS.gene83428:CDS | 50.0% | |
| ATTGAAGGCTATGGGGAGTG+TGG | + | chr7.2:22125861-22125880 | MS.gene83428:CDS | 50.0% | |
| CCCATAGCCTTCAATCCTCT+TGG | - | chr7.2:22125856-22125875 | None:intergenic | 50.0% | |
| GATCGATACTACACGACGAG+GGG | + | chr7.2:22125326-22125345 | MS.gene83428:CDS | 50.0% | |
| GCCAAGAGGATTGAAGGCTA+TGG | + | chr7.2:22125852-22125871 | MS.gene83428:CDS | 50.0% | |
| TGGAACTATTCTACGACCGG+CGG | + | chr7.2:22125265-22125284 | MS.gene83428:CDS | 50.0% | |
| !! | AGTTGGTAAGTCCTGGAGCA+TGG | + | chr7.2:22125666-22125685 | MS.gene83428:intron | 50.0% |
| !!! | GCCCTCTTTTTGGGTCTTTG+AGG | - | chr7.2:22125491-22125510 | None:intergenic | 50.0% |
| GCAGAGGCCAAGAGGATTGA+AGG | + | chr7.2:22125846-22125865 | MS.gene83428:intron | 55.0% | |
| TCAGCAGAGGACACCGGTAA+AGG | + | chr7.2:22125350-22125369 | MS.gene83428:CDS | 55.0% | |
| TTTCTGTTATCTCCGGCCGC+CGG | - | chr7.2:22125284-22125303 | None:intergenic | 55.0% | |
| ACTATTCTACGACCGGCGGC+CGG | + | chr7.2:22125269-22125288 | MS.gene83428:CDS | 60.0% | |
| AGGGGGTCAGCAGAGGACAC+CGG | + | chr7.2:22125344-22125363 | MS.gene83428:CDS | 65.0% | |
| CACGACGAGGGGGTCAGCAG+AGG | + | chr7.2:22125337-22125356 | MS.gene83428:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 22125256 | 22125900 | 22125256 | ID=MS.gene83428 |
| chr7.2 | mRNA | 22125256 | 22125900 | 22125256 | ID=MS.gene83428.t1;Parent=MS.gene83428 |
| chr7.2 | exon | 22125256 | 22125535 | 22125256 | ID=MS.gene83428.t1.exon1;Parent=MS.gene83428.t1 |
| chr7.2 | CDS | 22125256 | 22125535 | 22125256 | ID=cds.MS.gene83428.t1;Parent=MS.gene83428.t1 |
| chr7.2 | exon | 22125668 | 22125745 | 22125668 | ID=MS.gene83428.t1.exon2;Parent=MS.gene83428.t1 |
| chr7.2 | CDS | 22125668 | 22125745 | 22125668 | ID=cds.MS.gene83428.t1;Parent=MS.gene83428.t1 |
| chr7.2 | exon | 22125850 | 22125900 | 22125850 | ID=MS.gene83428.t1.exon3;Parent=MS.gene83428.t1 |
| chr7.2 | CDS | 22125850 | 22125900 | 22125850 | ID=cds.MS.gene83428.t1;Parent=MS.gene83428.t1 |
| Gene Sequence |
| Protein sequence |
